MD09G1205300.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0015897
Physical Location & Seq
Forward (+)
19575103 .. 19575690
588 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1205300.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGCACCTCATGGAGAGTCTATTTCTGGCTCTAACTACTCTAGGGCCAACAACACCTCCATGTCTAAGCCACCAAGGCTTTCATTCTCATCCGATAGCCTGAAAAGAACGATGTCCGACATCTCGTATGAGCTAAGCAAAGAAGCCGACATTGATCAGGACCTGAAATCCCTCCCGCCAATATCGGAGGTTGAAGATGCAAAGTGTGAATGCTGTGGCATGAGTGAAGAGTACACGCCCGAGTACATAGACCGAGTGAGAGACAAGTTCTTTGGGAAGTGGATATGCGGGTTGTGTTCTGAGGCAGTGAAAGAAGAGGTGGAGAAAAATGGAGGAAATAAGGAAGAGGCTTTAAATTCACATATAAGTGCATGTGTTAGGTTTAACAAGTATACTAGGGCGTATCCAGTGTTGTTTCAAGCTGAGGCTATGAGGGAGATGTTGAAGAAGAGCAAGTTGGAGGGGAGAGTCATGAGGGCTAAGTCGTTTAATCCGAGAGACAAAGGAGAGCCAAAGGGGAAGATTACTCGGAGTTCAAGCTGCATTCCAGCGATCACGAGGGAGATGAACGATCTCACCATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

196

Amino Acids

21.93

Weight (kDa)

7.52

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 64 - 152 1.7e-36 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015897)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G79770
fragaria_vesca FvH4_6g32260
malus_domestica MD09G1205300.v1.1 MD17G1185200.v1.1
prunus_persica Prupe.3G060600_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0140141
rosa_laevigata RLG00000019855
rosa_multiflora Rmu_sc0003244.1_g000018
rosa_roxburghii Rroxscaffold_2G00105010
rosa_rugosa Rorug02G0361400
rosa_samantha Rh2AG412100 Rh2BG422500 Rh2CG398200 Rh2DG431800
rosa_wichuraiana Rw2G033770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 392
AciI CCGC 2 cut(s) 176, 288
AcsI RAATTY 1 cut(s) 355
AfaI GTAC 2 cut(s) 233, 245
AgsI TTSAA 4 cut(s) 194, 419, 445, 537
AjuI GAANNNNNNNTTGG 2 cut(s) 440, 472
AluBI AGCT 3 cut(s) 133, 422, 540
AluI AGCT 3 cut(s) 133, 422, 540
Alw26I GTCTC 2 cut(s) 255, 492
AlwNI CAGNNNCTG 1 cut(s) 163
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 540
ApoI RAATTY 1 cut(s) 355
ArsI GACNNNNNNTTYG 2 cut(s) 254, 286
AspS9I GGNCC 2 cut(s) 45, 160
AsuHPI GGTGA 1 cut(s) 568
AvaI CYCGRG 1 cut(s) 239
AvaII GGWCC 1 cut(s) 160
BanI GGYRCC 1 cut(s) 4
BauI CACGAG 1 cut(s) 556
BbvCI CCTCAGC 1 cut(s) 423
BbvI GCAGC 1 cut(s) 527
BciVI GTATCC 1 cut(s) 414
BclI TGATCA 1 cut(s) 154
BcoDI GTCTC 2 cut(s) 255, 492
BfaI CTAG 2 cut(s) 42, 396
BfuI GTATCC 1 cut(s) 414
BglI GCCNNNNNGGC 1 cut(s) 76
BisI GCNGC 1 cut(s) 541
BlpI GCTNAGC 1 cut(s) 134
BlsI GCNGC 1 cut(s) 542
Bme18I GGWCC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 239
BmgT120I GGNCC 2 cut(s) 45, 160
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 1 cut(s) 187
Bpu10I CCTNAGC 1 cut(s) 423
Bpu1102I GCTNAGC 1 cut(s) 134
BsaJI CCNNGG 2 cut(s) 74, 579
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bse1I ACTGG 1 cut(s) 407
BseDI CCNNGG 2 cut(s) 74, 579
BseGI GGATG 1 cut(s) 89
BseMII CTCAG 2 cut(s) 291, 414
BseNI ACTGG 1 cut(s) 407
BseXI GCAGC 1 cut(s) 527
BshFI GGCC 1 cut(s) 47
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 1 cut(s) 239
BsmAI GTCTC 2 cut(s) 255, 492
BsmI GAATGC 2 cut(s) 215, 543
BsnI GGCC 1 cut(s) 47
BsoBI CYCGRG 1 cut(s) 239
Bsp143I GATC 3 cut(s) 154, 552, 571
Bsp1720I GCTNAGC 1 cut(s) 134
Bsp19I CCATGG 1 cut(s) 579
BspACI CCGC 2 cut(s) 176, 288
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 2 cut(s) 292, 415
BspHI TCATGA 1 cut(s) 471
BspLI GGNNCC 1 cut(s) 6
BspQI GCTCTTC 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 407
BssECI CCNNGG 2 cut(s) 74, 579
BssMI GATC 3 cut(s) 154, 552, 571
BssNAI GTATAC 1 cut(s) 393
BssSI CACGAG 1 cut(s) 556
BssT1I CCWWGG 2 cut(s) 74, 579
Bst1107I GTATAC 1 cut(s) 393
Bst2BI CACGAG 1 cut(s) 556
Bst6I CTCTTC 4 cut(s) 222, 309, 339, 443
BstDEI CTNAG 5 cut(s) 66, 134, 300, 423, 480
BstDSI CCRYGG 1 cut(s) 579
BstF5I GGATG 1 cut(s) 89
BstKTI GATC 3 cut(s) 157, 555, 574
BstMAI GTCTC 2 cut(s) 255, 492
BstMBI GATC 3 cut(s) 154, 552, 571
BstMWI GCNNNNNNNGC 1 cut(s) 76
BstNSI RCATGY 1 cut(s) 375
BstV1I GCAGC 1 cut(s) 527
BstZ17I GTATAC 1 cut(s) 393
BsuI GTATCC 1 cut(s) 414
BsuRI GGCC 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 579
BtsCI GGATG 1 cut(s) 89
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 312, 414
CaiI CAGNNNCTG 1 cut(s) 163
CciI TCATGA 1 cut(s) 471
Cfr13I GGNCC 2 cut(s) 45, 160
Csp6I GTAC 2 cut(s) 232, 244
CviAII CATG 6 cut(s) 11, 61, 220, 372, 472, 580
CviQI GTAC 2 cut(s) 232, 244
DdeI CTNAG 5 cut(s) 66, 134, 300, 423, 480
DpnI GATC 3 cut(s) 156, 554, 573
DpnII GATC 3 cut(s) 154, 552, 571
DraI TTTAAA 1 cut(s) 354
Eam1104I CTCTTC 4 cut(s) 222, 309, 339, 443
EarI CTCTTC 4 cut(s) 222, 309, 339, 443
Eco130I CCWWGG 2 cut(s) 74, 579
Eco47I GGWCC 1 cut(s) 160
Eco88I CYCGRG 1 cut(s) 239
EcoO109I RGGNCCY 1 cut(s) 160
EcoT14I CCWWGG 2 cut(s) 74, 579
ErhI CCWWGG 2 cut(s) 74, 579
FaeI CATG 6 cut(s) 14, 64, 223, 375, 475, 583
FatI CATG 6 cut(s) 10, 60, 219, 371, 471, 579
FauI CCCGC 2 cut(s) 183, 281
FbaI TGATCA 1 cut(s) 154
FblI GTMKAC 1 cut(s) 392
Fnu4HI GCNGC 1 cut(s) 541
FokI GGATG 1 cut(s) 76
Fsp4HI GCNGC 1 cut(s) 541
FspBI CTAG 2 cut(s) 42, 396
GluI GCNGC 1 cut(s) 541
HaeIII GGCC 1 cut(s) 47
Hin1II CATG 6 cut(s) 14, 64, 223, 375, 475, 583
HinfI GANTC 2 cut(s) 17, 468
HphI GGTGA 1 cut(s) 568
Hpy166II GTNNAC 2 cut(s) 234, 393
Hpy188I TCNGA 6 cut(s) 94, 118, 187, 301, 495, 531
Hpy188III TCNNGA 3 cut(s) 158, 472, 556
Hpy8I GTNNAC 2 cut(s) 234, 393
HpyCH4V TGCA 3 cut(s) 200, 371, 543
HpyF10VI GCNNNNNNNGC 1 cut(s) 76
HpyF3I CTNAG 5 cut(s) 66, 134, 300, 423, 480
Hsp92II CATG 6 cut(s) 14, 64, 223, 375, 475, 583
Ksp22I TGATCA 1 cut(s) 154
Kzo9I GATC 3 cut(s) 154, 552, 571
LguI GCTCTTC 1 cut(s) 443
LpnPI CCDG 6 cut(s) 12, 113, 143, 176, 420, 561
Lsp1109I GCAGC 1 cut(s) 527
LweI GCATC 1 cut(s) 187
MaeI CTAG 2 cut(s) 42, 396
MalI GATC 3 cut(s) 156, 554, 573
MboI GATC 3 cut(s) 154, 552, 571
MboII GAAGA 7 cut(s) 206, 239, 326, 356, 457, 460, 532
MluCI AATT 1 cut(s) 355
MlyI GAGTC 2 cut(s) 26, 477
MmeI TCCRAC 2 cut(s) 141, 438
MseI TTAA 3 cut(s) 353, 384, 489
MslI CAYNNNNRTG 2 cut(s) 59, 366
Mva1269I GAATGC 2 cut(s) 215, 543
MwoI GCNNNNNNNGC 1 cut(s) 76
NcoI CCATGG 1 cut(s) 579
NdeII GATC 3 cut(s) 154, 552, 571
NlaIII CATG 6 cut(s) 14, 64, 223, 375, 475, 583
NlaIV GGNNCC 1 cut(s) 6
NspI RCATGY 1 cut(s) 375
PagI TCATGA 1 cut(s) 471
PciSI GCTCTTC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 1 cut(s) 491
PctI GAATGC 2 cut(s) 215, 543
PkrI GCNGC 1 cut(s) 542
PleI GAGTC 2 cut(s) 25, 476
PpsI GAGTC 2 cut(s) 25, 476
PpuMI RGGWCCY 1 cut(s) 160
Psp5II RGGWCCY 1 cut(s) 160
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 45, 160
PspPPI RGGWCCY 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 163
RsaI GTAC 2 cut(s) 233, 245
RsaNI GTAC 2 cut(s) 232, 244
RseI CAYNNNNRTG 2 cut(s) 59, 366
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 3 cut(s) 353, 384, 489
SatI GCNGC 1 cut(s) 541
Sau3AI GATC 3 cut(s) 154, 552, 571
Sau96I GGNCC 2 cut(s) 45, 160
SchI GAGTC 2 cut(s) 26, 477
SetI ASST 9 cut(s) 10, 59, 135, 165, 192, 321, 383, 424, 542
SfaNI GCATC 1 cut(s) 187
SinI GGWCC 1 cut(s) 160
SmiMI CAYNNNNRTG 2 cut(s) 59, 366
Sse9I AATT 1 cut(s) 355
SsiI CCGC 2 cut(s) 176, 288
SspMI CTAG 2 cut(s) 42, 396
StyI CCWWGG 2 cut(s) 74, 579
TaqII GACCGA 1 cut(s) 267
TasI AATT 1 cut(s) 355
TatI WGTACW 2 cut(s) 231, 243
Tru1I TTAA 3 cut(s) 353, 384, 489
Tru9I TTAA 3 cut(s) 353, 384, 489
TscAI CASTG 2 cut(s) 312, 414
TseI GCWGC 1 cut(s) 540
TspDTI ATGAA 2 cut(s) 72, 581
TspRI CASTG 2 cut(s) 312, 414
VpaK11BI GGWCC 1 cut(s) 160
XapI RAATTY 1 cut(s) 355
XceI RCATGY 1 cut(s) 375
XmiI GTMKAC 1 cut(s) 392
XspI CTAG 2 cut(s) 42, 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.