FvH4_6g35250

Plant protein of unknown function (DUF936)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013133
Physical Location & Seq
Reverse (-)
27851192 .. 27854896
3705 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35250.t1

Sequence Viewer

Length: 1587 bp
ATGGCCTCCCTTACACCAGGAGTACTACTAAAGCTTCTTCAGAGCATGAACTCCAACATAAAGGTTCTTGGGGAATATCGGTCAGTCCTGCTACAAGTTATTAGTATTGTCCCTGCCTTGAGTGGCTCAGAACTTTGGCCTAATCAAGGCTTCTTTGTAAAAGTCTCGGATTCTTCTCATTCGACTTATGTCTCCCTCTCAAAGGAGGACAATGAGCTTATATTGAACAATAAGTTGCAGCTTGGCCAGTTCTTCTATGTTGATAGACTGGAGGCAGGAACACCAGTTCCCATTCTTCAAGGGGTCAGGCCAGTTCTGGGACGACACCCTTTTGTGGGGAACCCGAAGGACTTGATGCAAATGGTGGATCCATCCGAGAGTCCAGTCCAAGCCGATGATCATGGAGGAAGCAATAGTTTGAAACCAAGGGAGTCCTCTGATGGTAAAGAAGAAACCTCAAGACAAAAGATTGTTATTAAAGAGGAAAAGACAGCTGTTGCATCTAGGTACATGCAAGGTTTTTCGACATCAAATTCAAAAGCTAACTCGCAAGATTGTAGTGGAAGTGGGAAGCAAAATGACAATGAGAGTGGTGGTAAGAAGGTTGGCATGACAAAAGGCAAACAGCAAGAGCTTAATGGTCAGGCACGATCGATAACTCCTTCCAACAGCAGAGCTGGTGCACTTTTATCAAAACCGGAAGCAGCTGCATCAAACACCAAGCAGACTGCTGTGCCTTCTAAGAGAACTTCTGAAAAACGTGCTTCAAGCAAACAGGAAAACATAAATTATAACTGTTTGTCAAACCGGAAAGAGAAAACTCTTCCACCAGAAGTAGCTTTGTGGGGTTCTCTGCCGGCTAACTTATTGAAGCCCGGAAAGGGAATGCTTAGAAGGCGAAATTTAGCTTCTTTGGTTGCAGTAGAAGCTCAGAAAGAAGCATCTAGAGCTGCAACTCTTGTCAAATGTCTCAGTATCTTTGCCGATCTATGCTCATCTGCATCACCACAAAATCCCCACCTCTCACTCACCAAGTTTTTCAGACTCCACCAGCTCATTGACCAACCAAATATTGCAGCTCCTTTAAAGGATAAATTAACTCCTTTAACTACAAAACAGCTGCCTCCAGATATCGGAAAGTTCAGCAAAAGGAGTGGCACAATTCATGGTCAAAGTGCATTGAAGCCTCCAAAGCTCCCCATCGAGTTAAGTGAAGCAGAGAAACTAGAATGGGCTAAAGGAGAGAGTACAAAAGGGATAGCAGAACTACAAGAACTTCTCTTAAATGAAACAAGGTCTTGGTTCTTGAAATTCTTGGAGGCAGAACTAGATGCTGGTGGCTTTCATCTGAATACACAAGAGAAGAAAGGCAAGGAAAGTGCAGCGCGACGGAATGAGCCAGACAATCATATTGCTGTGACATTGTCACAGCTGAAAAATTCAAATGAGTGGTTGGATAAACTAAAAACTAGTCTGAGTTCAGAGAGCAGTGGATTGGTGGAGACAGTTGACAGGTTGAAACAAAAAGTATATGCTTGTTTACTTGTTCATGTGGAGTCTGCTGCTTCAGCTCTCGAAAATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF936

Protein Analysis

529

Amino Acids

57.64

Weight (kDa)

9.38

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF936 PF06075 4 - 119 5.2e-53 Plant protein of unknown function (DUF936)
DUF6857 PF21647 280 - 360 1.5e-21 Domain of unknown function (DUF6857)
DUF6857 PF21647 384 - 525 2.6e-28 Domain of unknown function (DUF6857)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14170 AT3G14170
fragaria_vesca FvH4_6g35250
malus_domestica MD09G1177200.v1.1 MD17G1149100.v1.1
prunus_persica Prupe.3G036500_v2.0.a1 Prupe.3G036500_v2.0.a1
pyrus_communis pycom09g09540
rosa_chinensis RchiOBHm_Chr2g0146611
rosa_laevigata RLG00000020210
rosa_multiflora Rmu_sc0001986.1_g000031
rosa_roxburghii Rroxscaffold_2G00100010
rosa_rugosa Rorug02G0395700
rosa_samantha Rh2AG451900 Rh2BG464700 Rh2CG438800 Rh2DG473700
rosa_wichuraiana Rw2G036920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 792
AccII CGCG 1 cut(s) 1387
AclWI GGATC 2 cut(s) 362, 375
AcoI YGGCCR 1 cut(s) 244
AcsI RAATTY 4 cut(s) 532, 901, 1310, 1438
AcuI CTGAAG 2 cut(s) 23, 1551
AfaI GTAC 3 cut(s) 24, 509, 1249
AfiI CCNNNNNNNGG 7 cut(s) 146, 202, 317, 334, 335, 881, 1133
AhlI ACTAGT 1 cut(s) 1469
AjnI CCWGG 1 cut(s) 16
AloI GAACNNNNNNTCC 4 cut(s) 270, 271, 302, 303
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 4 cut(s) 169, 196, 974, 1496
Alw44I GTGCAC 1 cut(s) 681
AlwI GGATC 2 cut(s) 362, 375
AoxI GGCC 4 cut(s) 3, 137, 244, 308
ApaLI GTGCAC 1 cut(s) 681
ApeKI GCWGC 8 cut(s) 238, 704, 707, 950, 1076, 1120, 1382, 1562
ApoI RAATTY 4 cut(s) 532, 901, 1310, 1438
AspLEI GCGC 1 cut(s) 1387
AsuC2I CCSGG 1 cut(s) 876
AsuHPI GGTGA 2 cut(s) 996, 1021
BaeGI GKGCMC 1 cut(s) 685
BalI TGGCCA 1 cut(s) 246
BamHI GGATCC 1 cut(s) 367
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 8 cut(s) 250, 694, 716, 937, 1088, 1107, 1394, 1549
BccI CCATC 3 cut(s) 379, 434, 1208
BciT130I CCWGG 1 cut(s) 18
BclI TGATCA 1 cut(s) 397
BcnI CCSGG 1 cut(s) 876
BcoDI GTCTC 4 cut(s) 169, 196, 974, 1496
BcuI ACTAGT 1 cut(s) 1469
BfaI CTAG 5 cut(s) 504, 945, 1226, 1328, 1470
BisI GCNGC 8 cut(s) 239, 705, 708, 951, 1077, 1121, 1383, 1563
BlsI GCNGC 8 cut(s) 240, 706, 709, 952, 1078, 1122, 1384, 1564
BmcAI AGTACT 1 cut(s) 24
Bme1390I CCNGG 2 cut(s) 18, 876
BmiI GGNNCC 2 cut(s) 341, 369
BmrFI CCNGG 2 cut(s) 18, 876
BmsI GCATC 6 cut(s) 345, 509, 719, 950, 1010, 1321
BoxI GACNNNNGTC 1 cut(s) 188
BpmI CTGGAG 2 cut(s) 290, 1110
BpuEI CTTGAG 2 cut(s) 139, 442
BpuMI CCSGG 1 cut(s) 876
Bsa29I ATCGAT 1 cut(s) 653
BsaJI CCNNGG 1 cut(s) 425
BsaWI WCCGGW 2 cut(s) 697, 807
Bsc4I CCNNNNNNNGG 7 cut(s) 146, 202, 317, 334, 335, 881, 1133
Bse118I RCCGGY 1 cut(s) 856
Bse1I ACTGG 5 cut(s) 247, 273, 284, 311, 383
BseBI CCWGG 1 cut(s) 18
BseCI ATCGAT 1 cut(s) 653
BseDI CCNNGG 1 cut(s) 425
BseGI GGATG 1 cut(s) 371
BseLI CCNNNNNNNGG 7 cut(s) 146, 202, 317, 334, 335, 881, 1133
BseMII CTCAG 4 cut(s) 141, 944, 985, 1466
BseNI ACTGG 5 cut(s) 247, 273, 284, 311, 383
BseSI GKGCMC 1 cut(s) 685
BseXI GCAGC 8 cut(s) 250, 694, 716, 937, 1088, 1107, 1394, 1549
BsgI GTGCAG 1 cut(s) 1401
Bsh1236I CGCG 1 cut(s) 1387
Bsh1285I CGRYCG 1 cut(s) 653
BshFI GGCC 4 cut(s) 5, 139, 246, 310
BshVI ATCGAT 1 cut(s) 653
BsiEI CGRYCG 1 cut(s) 653
BsiHKAI GWGCWC 1 cut(s) 685
BsiSI CCGG 4 cut(s) 698, 808, 857, 876
BslFI GGGAC 2 cut(s) 95, 333
BslI CCNNNNNNNGG 7 cut(s) 146, 202, 317, 334, 335, 881, 1133
BsmAI GTCTC 4 cut(s) 169, 196, 974, 1496
BsmFI GGGAC 2 cut(s) 95, 333
BsmI GAATGC 1 cut(s) 891
BsnI GGCC 4 cut(s) 5, 139, 246, 310
Bsp1286I GDGCHC 1 cut(s) 685
Bsp143I GATC 4 cut(s) 367, 397, 650, 985
BspANI GGCC 4 cut(s) 5, 139, 246, 310
BspCNI CTCAG 4 cut(s) 140, 943, 984, 1467
BspDI ATCGAT 1 cut(s) 653
BspFNI CGCG 1 cut(s) 1387
BspLI GGNNCC 2 cut(s) 341, 369
BspPI GGATC 2 cut(s) 362, 375
BsrFI RCCGGY 1 cut(s) 856
BsrI ACTGG 5 cut(s) 247, 273, 284, 311, 383
BssAI RCCGGY 1 cut(s) 856
BssECI CCNNGG 1 cut(s) 425
BssMI GATC 4 cut(s) 367, 397, 650, 985
BssT1I CCWWGG 1 cut(s) 425
Bst2UI CCWGG 1 cut(s) 18
Bst4CI ACNGT 2 cut(s) 797, 1507
Bst6I CTCTTC 1 cut(s) 828
BstC8I GCNNGC 1 cut(s) 858
BstDEI CTNAG 6 cut(s) 127, 741, 890, 930, 971, 1475
BstENI CCTNNNNNAGG 2 cut(s) 144, 200
BstF5I GGATG 1 cut(s) 371
BstFNI CGCG 1 cut(s) 1387
BstHHI GCGC 1 cut(s) 1387
BstKTI GATC 4 cut(s) 370, 400, 653, 988
BstMAI GTCTC 4 cut(s) 169, 196, 974, 1496
BstMBI GATC 4 cut(s) 367, 397, 650, 985
BstMCI CGRYCG 1 cut(s) 653
BstMWI GCNNNNNNNGC 5 cut(s) 895, 926, 947, 1192, 1568
BstNI CCWGG 1 cut(s) 18
BstNSI RCATGY 1 cut(s) 514
BstPAI GACNNNNGTC 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 16, 874
BstSLI GKGCMC 1 cut(s) 685
BstUI CGCG 1 cut(s) 1387
BstV1I GCAGC 8 cut(s) 250, 694, 716, 937, 1088, 1107, 1394, 1549
BstX2I RGATCY 1 cut(s) 367
BstYI RGATCY 1 cut(s) 367
Bsu15I ATCGAT 1 cut(s) 653
BsuRI GGCC 4 cut(s) 5, 139, 246, 310
BsuTUI ATCGAT 1 cut(s) 653
BtsCI GGATG 1 cut(s) 371
BtsI GCAGTG 1 cut(s) 1495
BtsIMutI CAGTG 1 cut(s) 1495
Cac8I GCNNGC 1 cut(s) 858
CfoI GCGC 1 cut(s) 1387
Cfr10I RCCGGY 1 cut(s) 856
ClaI ATCGAT 1 cut(s) 653
Csp6I GTAC 3 cut(s) 23, 508, 1248
CspCI CAANNNNNGTGG 4 cut(s) 571, 606, 1135, 1170
CviAII CATG 6 cut(s) 46, 401, 511, 610, 1166, 1550
CviQI GTAC 3 cut(s) 23, 508, 1248
DdeI CTNAG 6 cut(s) 127, 741, 890, 930, 971, 1475
DpnI GATC 4 cut(s) 369, 399, 652, 987
DpnII GATC 4 cut(s) 367, 397, 650, 985
DraI TTTAAA 1 cut(s) 1086
EaeI YGGCCR 1 cut(s) 244
Eam1104I CTCTTC 1 cut(s) 828
EarI CTCTTC 1 cut(s) 828
Eco130I CCWWGG 1 cut(s) 425
Eco32I GATATC 1 cut(s) 1132
Eco57I CTGAAG 2 cut(s) 23, 1551
EcoNI CCTNNNNNAGG 2 cut(s) 144, 200
EcoRII CCWGG 1 cut(s) 16
EcoRV GATATC 1 cut(s) 1132
EcoT14I CCWWGG 1 cut(s) 425
ErhI CCWWGG 1 cut(s) 425
FaeI CATG 6 cut(s) 49, 404, 514, 613, 1169, 1553
FaqI GGGAC 2 cut(s) 95, 333
FatI CATG 6 cut(s) 45, 400, 510, 609, 1165, 1549
FbaI TGATCA 1 cut(s) 397
Fnu4HI GCNGC 8 cut(s) 239, 705, 708, 951, 1077, 1121, 1383, 1563
FokI GGATG 1 cut(s) 358
Fsp4HI GCNGC 8 cut(s) 239, 705, 708, 951, 1077, 1121, 1383, 1563
FspBI CTAG 5 cut(s) 504, 945, 1226, 1328, 1470
GlaI GCGC 1 cut(s) 1386
GluI GCNGC 8 cut(s) 239, 705, 708, 951, 1077, 1121, 1383, 1563
GsuI CTGGAG 2 cut(s) 290, 1110
HaeIII GGCC 4 cut(s) 5, 139, 246, 310
HapII CCGG 4 cut(s) 698, 808, 857, 876
HhaI GCGC 1 cut(s) 1387
Hin1II CATG 6 cut(s) 49, 404, 514, 613, 1169, 1553
Hin6I GCGC 1 cut(s) 1385
HinP1I GCGC 1 cut(s) 1385
HincII GTYRAC 1 cut(s) 1510
HindII GTYRAC 1 cut(s) 1510
HindIII AAGCTT 1 cut(s) 32
HinfI GANTC 5 cut(s) 170, 379, 431, 1044, 1556
HpaII CCGG 4 cut(s) 698, 808, 857, 876
HphI GGTGA 2 cut(s) 996, 1021
Hpy166II GTNNAC 3 cut(s) 683, 1510, 1541
Hpy188III TCNNGA 5 cut(s) 459, 945, 1127, 1306, 1574
Hpy8I GTNNAC 3 cut(s) 683, 1510, 1541
Hpy99I CGWCG 1 cut(s) 1392
HpyAV CCTTC 5 cut(s) 340, 595, 672, 747, 888
HpyCH4III ACNGT 2 cut(s) 797, 1507
HpyCH4IV ACGT 1 cut(s) 760
HpyF10VI GCNNNNNNNGC 5 cut(s) 895, 926, 947, 1192, 1568
HpyF3I CTNAG 6 cut(s) 127, 741, 890, 930, 971, 1475
HpySE526I ACGT 1 cut(s) 760
Hsp92II CATG 6 cut(s) 49, 404, 514, 613, 1169, 1553
HspAI GCGC 1 cut(s) 1385
KroI GCCGGC 1 cut(s) 856
KroNI GCCGGC 1 cut(s) 858
Ksp22I TGATCA 1 cut(s) 397
Kzo9I GATC 4 cut(s) 367, 397, 650, 985
LmnI GCTCC 2 cut(s) 1084, 1200
Lsp1109I GCAGC 8 cut(s) 250, 694, 716, 937, 1088, 1107, 1394, 1549
LweI GCATC 6 cut(s) 345, 509, 719, 950, 1010, 1321
MaeI CTAG 5 cut(s) 504, 945, 1226, 1328, 1470
MaeII ACGT 1 cut(s) 760
MaeIII GTNAC 2 cut(s) 1417, 1425
MalI GATC 4 cut(s) 369, 399, 652, 987
MboI GATC 4 cut(s) 367, 397, 650, 985
MboII GAAGA 7 cut(s) 29, 165, 244, 287, 461, 815, 1375
MflI RGATCY 1 cut(s) 367
MhlI GDGCHC 1 cut(s) 685
MlsI TGGCCA 1 cut(s) 246
MluCI AATT 7 cut(s) 532, 787, 901, 1094, 1161, 1310, 1438
MluNI TGGCCA 1 cut(s) 246
MlyI GAGTC 4 cut(s) 388, 440, 1038, 1565
MmeI TCCRAC 3 cut(s) 78, 690, 1434
Mox20I TGGCCA 1 cut(s) 246
MroNI GCCGGC 1 cut(s) 856
MscI TGGCCA 1 cut(s) 246
MseI TTAA 7 cut(s) 477, 636, 1085, 1097, 1106, 1208, 1283
Msp20I TGGCCA 1 cut(s) 246
MspA1I CMGCKG 4 cut(s) 494, 707, 1120, 1432
MspI CCGG 4 cut(s) 698, 808, 857, 876
MspR9I CCNGG 2 cut(s) 18, 876
Mva1269I GAATGC 1 cut(s) 891
MvaI CCWGG 1 cut(s) 18
MvnI CGCG 1 cut(s) 1387
MwoI GCNNNNNNNGC 5 cut(s) 895, 926, 947, 1192, 1568
NaeI GCCGGC 1 cut(s) 858
NciI CCSGG 1 cut(s) 876
NdeII GATC 4 cut(s) 367, 397, 650, 985
NgoMIV GCCGGC 1 cut(s) 856
NlaIII CATG 6 cut(s) 49, 404, 514, 613, 1169, 1553
NlaIV GGNNCC 2 cut(s) 341, 369
NmuCI GTSAC 2 cut(s) 1417, 1425
NspI RCATGY 1 cut(s) 514
PctI GAATGC 1 cut(s) 891
PdiI GCCGGC 1 cut(s) 858
PfeI GAWTC 1 cut(s) 170
PflFI GACNNNGTC 1 cut(s) 1423
PkrI GCNGC 8 cut(s) 240, 706, 709, 952, 1078, 1122, 1384, 1564
Ple19I CGATCG 1 cut(s) 653
PleI GAGTC 4 cut(s) 387, 439, 1038, 1564
PpsI GAGTC 4 cut(s) 387, 439, 1038, 1564
PshAI GACNNNNGTC 1 cut(s) 188
PsiI TTATAA 1 cut(s) 792
Psp6I CCWGG 1 cut(s) 16
PspGI CCWGG 1 cut(s) 16
PspN4I GGNNCC 2 cut(s) 341, 369
PsuI RGATCY 1 cut(s) 367
PsyI GACNNNGTC 1 cut(s) 1423
PvuI CGATCG 1 cut(s) 653
PvuII CAGCTG 4 cut(s) 494, 707, 1120, 1432
RsaI GTAC 3 cut(s) 24, 509, 1249
RsaNI GTAC 3 cut(s) 23, 508, 1248
SaqAI TTAA 7 cut(s) 477, 636, 1085, 1097, 1106, 1208, 1283
SatI GCNGC 8 cut(s) 239, 705, 708, 951, 1077, 1121, 1383, 1563
Sau3AI GATC 4 cut(s) 367, 397, 650, 985
ScaI AGTACT 1 cut(s) 24
SchI GAGTC 4 cut(s) 388, 440, 1038, 1565
ScrFI CCNGG 2 cut(s) 18, 876
SduI GDGCHC 1 cut(s) 685
SfaNI GCATC 6 cut(s) 345, 509, 719, 950, 1010, 1321
SmlI CTYRAG 2 cut(s) 118, 457
SmoI CTYRAG 2 cut(s) 118, 457
SpeI ACTAGT 1 cut(s) 1469
Sse9I AATT 7 cut(s) 532, 787, 901, 1094, 1161, 1310, 1438
SspI AATATT 1 cut(s) 1072
SspMI CTAG 5 cut(s) 504, 945, 1226, 1328, 1470
StyD4I CCNGG 2 cut(s) 16, 874
StyI CCWWGG 1 cut(s) 425
TaaI ACNGT 2 cut(s) 797, 1507
TaiI ACGT 1 cut(s) 763
TaqI TCGA 5 cut(s) 182, 524, 653, 1203, 1575
TaqII GACCGA 1 cut(s) 69
TasI AATT 7 cut(s) 532, 787, 901, 1094, 1161, 1310, 1438
TatI WGTACW 2 cut(s) 22, 1247
TfiI GAWTC 1 cut(s) 170
Tru1I TTAA 7 cut(s) 477, 636, 1085, 1097, 1106, 1208, 1283
Tru9I TTAA 7 cut(s) 477, 636, 1085, 1097, 1106, 1208, 1283
TscAI CASTG 1 cut(s) 1495
TseFI GTSAC 2 cut(s) 1417, 1425
TseI GCWGC 8 cut(s) 238, 704, 707, 950, 1076, 1120, 1382, 1562
Tsp45I GTSAC 2 cut(s) 1417, 1425
TspDTI ATGAA 5 cut(s) 62, 1154, 1302, 1334, 1538
TspGWI ACGGA 1 cut(s) 1405
TspRI CASTG 1 cut(s) 1495
Tth111I GACNNNGTC 1 cut(s) 1423
VneI GTGCAC 1 cut(s) 681
XagI CCTNNNNNAGG 2 cut(s) 144, 200
XapI RAATTY 4 cut(s) 532, 901, 1310, 1438
XbaI TCTAGA 1 cut(s) 944
XceI RCATGY 1 cut(s) 514
XspI CTAG 5 cut(s) 504, 945, 1226, 1328, 1470
ZrmI AGTACT 1 cut(s) 24
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.