FvH4_6g36220

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017699
Physical Location & Seq
Forward (+)
28471714 .. 28475235
3522 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g36220.t1

Sequence Viewer

Length: 807 bp
ATGGGATTCTTAGAAGCCTTAGGATTGGGAAAGAGAACTACTCCTGTAACCTTCCACAATCATCTTCAGATAAGAGATCGATACAAAGTACCTTTAGAAGGAACCGAAGCATCATCTTCTAATTCTGAGGTGGCCATTACTACTCCAAACGAAAATTTTCCAACAGAAACGATAGCAGTGAGTGCATACTATACTGTAAGAACAAAGCTCAAGAGCTTACTAGAGGATCATCAAAATGCAAAGTTCGCAGTTACAGGCCATAGCCTAGGTGGCGCACTAGCCTCATTGTTCATTTCGGTGCTGGTATTGCATGGGGAGATGAATGTGATACAAAGGCTGCTTGGTGTCTACACATTTGCGCAACCAAGGATAGGAGATTACCAGCTGGGGCGTTACATTGAGCCACATTTGAATAATCCGGTCCCAAAGTACTTCAGGGTTGTCTACTGCAATGATTTTCTGCCCAGGCTTCCTTATGATAAACGGAACTGCTTCTATAAGCATTTTGGGGTGTGCCTTTACTATGATAGTCGATATATTGAACAAGTAATGGAGGAGGAGCAGCCAAACAGAAATTACTTTGGAATGAGACATCTCATGGCACATATTCTGAATGCTGCTTGGGAGCTAATGAGAAGTTTGACAATGGGGAACATATATGGACCAGCTTATGCAGAAGGGTGGCTCTCCATTTTGGTAAGGACAAACGGACTTGTGTTTCCTCGCATGTCGGCACATTTACTTTCAGATTACATCAACTCGGTGAGGATTGGGAAGGAGGGCATCATTCAAGTGTCCTCTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

269

Amino Acids

30.52

Weight (kDa)

8.65

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 61 - 160 3e-26 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017699)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g36220
malus_domestica MD17G1155700.v1.1
rosa_chinensis RchiOBHm_Chr2g0148191
rosa_laevigata RLG00000020325
rosa_multiflora Rmu_co8278977.1_g000001 Rmu_sc0001516.1_g000104
rosa_roxburghii Rroxscaffold_2G00099010
rosa_rugosa Rorug02G0404300.1
rosa_samantha Rh2BG476100 Rh2DG484800 Rh6BG483600 Rh6DG474800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 360
AccI GTMKAC 2 cut(s) 348, 444
AclWI GGATC 1 cut(s) 234
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 1 cut(s) 154
AcuI CTGAAG 2 cut(s) 50, 418
AfaI GTAC 2 cut(s) 90, 431
AfiI CCNNNNNNNGG 2 cut(s) 98, 371
AgsI TTSAA 3 cut(s) 412, 542, 791
AjnI CCWGG 1 cut(s) 464
AluBI AGCT 5 cut(s) 208, 216, 385, 628, 668
AluI AGCT 5 cut(s) 208, 216, 385, 628, 668
Alw26I GTCTC 1 cut(s) 583
AlwI GGATC 1 cut(s) 234
AoxI GGCC 2 cut(s) 132, 256
ApeKI GCWGC 3 cut(s) 337, 562, 617
ApoI RAATTY 1 cut(s) 154
Asp700I GAANNNNTTC 2 cut(s) 156, 491
AspA2I CCTAGG 1 cut(s) 265
AspLEI GCGC 2 cut(s) 275, 361
AspS9I GGNCC 2 cut(s) 421, 662
AsuHPI GGTGA 1 cut(s) 775
AvaII GGWCC 2 cut(s) 421, 662
AvrII CCTAGG 1 cut(s) 265
AxyI CCTNAGG 1 cut(s) 19
BalI TGGCCA 1 cut(s) 134
BbvI GCAGC 3 cut(s) 324, 574, 604
BciT130I CCWGG 1 cut(s) 466
BcoDI GTCTC 1 cut(s) 583
BfaI CTAG 3 cut(s) 221, 266, 278
BglI GCCNNNNNGGC 1 cut(s) 270
BisI GCNGC 3 cut(s) 338, 563, 618
BlnI CCTAGG 1 cut(s) 265
BlsI GCNGC 3 cut(s) 339, 564, 619
BmcAI AGTACT 1 cut(s) 431
Bme1390I CCNGG 1 cut(s) 466
Bme18I GGWCC 2 cut(s) 421, 662
BmgT120I GGNCC 2 cut(s) 421, 662
BmiI GGNNCC 2 cut(s) 103, 423
BmrFI CCNGG 1 cut(s) 466
BmsI GCATC 2 cut(s) 119, 792
BplI GAGNNNNNCTC 2 cut(s) 25, 57
BpuEI CTTGAG 1 cut(s) 194
Bsa29I ATCGAT 1 cut(s) 79
BsaJI CCNNGG 3 cut(s) 265, 365, 464
BsaWI WCCGGW 1 cut(s) 418
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 371
Bse21I CCTNAGG 1 cut(s) 19
Bse3DI GCAATG 1 cut(s) 457
BseBI CCWGG 1 cut(s) 466
BseCI ATCGAT 1 cut(s) 79
BseDI CCNNGG 3 cut(s) 265, 365, 464
BseLI CCNNNNNNNGG 2 cut(s) 98, 371
BseMI GCAATG 1 cut(s) 457
BseMII CTCAG 1 cut(s) 117
BseRI GAGGAG 2 cut(s) 569, 572
BseXI GCAGC 3 cut(s) 324, 574, 604
BseYI CCCAGC 1 cut(s) 385
BshFI GGCC 2 cut(s) 134, 258
BshVI ATCGAT 1 cut(s) 79
BsiSI CCGG 1 cut(s) 419
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 2 cut(s) 98, 371
BsmAI GTCTC 1 cut(s) 583
BsmFI GGGAC 1 cut(s) 407
BsmI GAATGC 1 cut(s) 619
BsnI GGCC 2 cut(s) 134, 258
Bsp143I GATC 2 cut(s) 76, 226
BspANI GGCC 2 cut(s) 134, 258
BspCNI CTCAG 1 cut(s) 118
BspDI ATCGAT 1 cut(s) 79
BspLI GGNNCC 2 cut(s) 103, 423
BspPI GGATC 1 cut(s) 234
BsrDI GCAATG 1 cut(s) 457
BssECI CCNNGG 3 cut(s) 265, 365, 464
BssMI GATC 2 cut(s) 76, 226
BssT1I CCWWGG 2 cut(s) 265, 365
Bst2UI CCWGG 1 cut(s) 466
Bst4CI ACNGT 1 cut(s) 196
BstAPI GCANNNNNTGC 1 cut(s) 182
BstDEI CTNAG 3 cut(s) 10, 19, 126
BstENI CCTNNNNNAGG 1 cut(s) 96
BstHHI GCGC 2 cut(s) 275, 361
BstKTI GATC 2 cut(s) 79, 229
BstMAI GTCTC 1 cut(s) 583
BstMBI GATC 2 cut(s) 76, 226
BstMWI GCNNNNNNNGC 4 cut(s) 182, 245, 270, 307
BstNI CCWGG 1 cut(s) 466
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 1 cut(s) 464
BstV1I GCAGC 3 cut(s) 324, 574, 604
Bsu15I ATCGAT 1 cut(s) 79
Bsu36I CCTNAGG 1 cut(s) 19
BsuRI GGCC 2 cut(s) 134, 258
BsuTUI ATCGAT 1 cut(s) 79
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 1 cut(s) 183
CfoI GCGC 2 cut(s) 275, 361
Cfr13I GGNCC 2 cut(s) 421, 662
ClaI ATCGAT 1 cut(s) 79
Csp6I GTAC 2 cut(s) 89, 430
CviAII CATG 3 cut(s) 311, 598, 727
CviQI GTAC 2 cut(s) 89, 430
DdeI CTNAG 3 cut(s) 10, 19, 126
DpnI GATC 2 cut(s) 78, 228
DpnII GATC 2 cut(s) 76, 226
EaeI YGGCCR 1 cut(s) 132
Eco130I CCWWGG 2 cut(s) 265, 365
Eco47I GGWCC 2 cut(s) 421, 662
Eco57I CTGAAG 2 cut(s) 50, 418
Eco81I CCTNAGG 1 cut(s) 19
EcoNI CCTNNNNNAGG 1 cut(s) 96
EcoRII CCWGG 1 cut(s) 464
EcoT14I CCWWGG 2 cut(s) 265, 365
ErhI CCWWGG 2 cut(s) 265, 365
FaeI CATG 3 cut(s) 314, 601, 730
FaqI GGGAC 1 cut(s) 407
FatI CATG 3 cut(s) 310, 597, 726
FblI GTMKAC 2 cut(s) 348, 444
Fnu4HI GCNGC 3 cut(s) 338, 563, 618
Fsp4HI GCNGC 3 cut(s) 338, 563, 618
FspBI CTAG 3 cut(s) 221, 266, 278
FspI TGCGCA 1 cut(s) 360
GlaI GCGC 2 cut(s) 274, 360
GluI GCNGC 3 cut(s) 338, 563, 618
GsaI CCCAGC 1 cut(s) 389
HaeIII GGCC 2 cut(s) 134, 258
HapII CCGG 1 cut(s) 419
HhaI GCGC 2 cut(s) 275, 361
Hin1II CATG 3 cut(s) 314, 601, 730
Hin6I GCGC 2 cut(s) 273, 359
HinP1I GCGC 2 cut(s) 273, 359
HinfI GANTC 1 cut(s) 6
HpaII CCGG 1 cut(s) 419
HphI GGTGA 1 cut(s) 775
Hpy166II GTNNAC 2 cut(s) 349, 445
Hpy188I TCNGA 4 cut(s) 69, 127, 612, 748
Hpy188III TCNNGA 1 cut(s) 211
Hpy8I GTNNAC 2 cut(s) 349, 445
HpyAV CCTTC 4 cut(s) 61, 92, 671, 769
HpyCH4III ACNGT 1 cut(s) 196
HpyCH4V TGCA 5 cut(s) 185, 239, 310, 450, 674
HpyF10VI GCNNNNNNNGC 4 cut(s) 182, 245, 270, 307
HpyF3I CTNAG 3 cut(s) 10, 19, 126
Hsp92II CATG 3 cut(s) 314, 601, 730
HspAI GCGC 2 cut(s) 273, 359
Kzo9I GATC 2 cut(s) 76, 226
LmnI GCTCC 2 cut(s) 559, 625
Lsp1109I GCAGC 3 cut(s) 324, 574, 604
LweI GCATC 2 cut(s) 119, 792
MaeI CTAG 3 cut(s) 221, 266, 278
MaeIII GTNAC 3 cut(s) 46, 250, 392
MalI GATC 2 cut(s) 78, 228
MboI GATC 2 cut(s) 76, 226
MboII GAAGA 3 cut(s) 56, 108, 792
MlsI TGGCCA 1 cut(s) 134
MluCI AATT 3 cut(s) 121, 154, 574
MluNI TGGCCA 1 cut(s) 134
MmeI TCCRAC 1 cut(s) 185
MnlI CCTC 8 cut(s) 121, 217, 292, 547, 550, 732, 759, 772
Mox20I TGGCCA 1 cut(s) 134
MroXI GAANNNNTTC 2 cut(s) 156, 491
MscI TGGCCA 1 cut(s) 134
MslI CAYNNNNRTG 3 cut(s) 234, 296, 791
Msp20I TGGCCA 1 cut(s) 134
MspA1I CMGCKG 1 cut(s) 385
MspI CCGG 1 cut(s) 419
MspR9I CCNGG 1 cut(s) 466
Mva1269I GAATGC 1 cut(s) 619
MvaI CCWGG 1 cut(s) 466
MwoI GCNNNNNNNGC 4 cut(s) 182, 245, 270, 307
NdeII GATC 2 cut(s) 76, 226
NlaIII CATG 3 cut(s) 314, 601, 730
NlaIV GGNNCC 2 cut(s) 103, 423
NsbI TGCGCA 1 cut(s) 360
NspI RCATGY 1 cut(s) 730
PctI GAATGC 1 cut(s) 619
PdmI GAANNNNTTC 2 cut(s) 156, 491
PfeI GAWTC 1 cut(s) 6
PkrI GCNGC 3 cut(s) 339, 564, 619
Psp6I CCWGG 1 cut(s) 464
PspFI CCCAGC 1 cut(s) 385
PspGI CCWGG 1 cut(s) 464
PspN4I GGNNCC 2 cut(s) 103, 423
PspPI GGNCC 2 cut(s) 421, 662
PvuII CAGCTG 1 cut(s) 385
RsaI GTAC 2 cut(s) 90, 431
RsaNI GTAC 2 cut(s) 89, 430
RseI CAYNNNNRTG 3 cut(s) 234, 296, 791
SatI GCNGC 3 cut(s) 338, 563, 618
Sau3AI GATC 2 cut(s) 76, 226
Sau96I GGNCC 2 cut(s) 421, 662
ScaI AGTACT 1 cut(s) 431
ScrFI CCNGG 1 cut(s) 466
SetI ASST 9 cut(s) 53, 94, 132, 210, 218, 271, 387, 630, 670
SfaNI GCATC 2 cut(s) 119, 792
SinI GGWCC 2 cut(s) 421, 662
SmiMI CAYNNNNRTG 3 cut(s) 234, 296, 791
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 3 cut(s) 121, 154, 574
SspMI CTAG 3 cut(s) 221, 266, 278
StyD4I CCNGG 1 cut(s) 464
StyI CCWWGG 2 cut(s) 265, 365
TaaI ACNGT 1 cut(s) 196
TaqI TCGA 2 cut(s) 79, 532
TasI AATT 3 cut(s) 121, 154, 574
TatI WGTACW 1 cut(s) 429
TfiI GAWTC 1 cut(s) 6
TscAI CASTG 1 cut(s) 183
TseI GCWGC 3 cut(s) 337, 562, 617
TspDTI ATGAA 2 cut(s) 280, 335
TspGWI ACGGA 2 cut(s) 499, 723
TspRI CASTG 1 cut(s) 183
VpaK11BI GGWCC 2 cut(s) 421, 662
XagI CCTNNNNNAGG 1 cut(s) 96
XapI RAATTY 1 cut(s) 154
XceI RCATGY 1 cut(s) 730
XcmI CCANNNNNNNNNTGG 1 cut(s) 266
XmaJI CCTAGG 1 cut(s) 265
XmiI GTMKAC 2 cut(s) 348, 444
XmnI GAANNNNTTC 2 cut(s) 156, 491
XspI CTAG 3 cut(s) 221, 266, 278
ZrmI AGTACT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.