RLG00000020325

Lipase (class 3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017699
Physical Location & Seq
Forward (+)
63738274 .. 63738621
348 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020325

Sequence Viewer

Length: 348 bp
ATGAGTGCATACTTCGCTGTAAGAACAAAGCTCAAGAGCTTACTAGAGGATCATAAAAATGCAAAGTTCGCAGTCACGGGGCATAGCCTAGGTGGCGCTCTTGCCTCATTGTTCACCTCGGTGCTGGTGTTGCATGGGGAGATGAATGTGATGCAAAGGCTGCTTGGTGTCCACACATTTGCACAACAAAGGATAGGAGATTGCCAGCTGGGGAGGTTCGTTGAGGCACATTTGATCAATCCGGGCCCAAAGTACTTCAGGGTCGTCAACTGCAATGATTTTTTGCCCAGGTTGCCTTATGATGAACGAAACTGCTTCTATAAGCATTTTGGGGTGTCTTTACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_3

Protein Analysis

116

Amino Acids

12.98

Weight (kDa)

9.37

Isoelectric Point (pI)

19.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 3 - 101 1.6e-24 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017699)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g36220
malus_domestica MD17G1155700.v1.1
rosa_chinensis RchiOBHm_Chr2g0148191
rosa_laevigata RLG00000020325
rosa_multiflora Rmu_co8278977.1_g000001 Rmu_sc0001516.1_g000104
rosa_roxburghii Rroxscaffold_2G00099010
rosa_rugosa Rorug02G0404300.1
rosa_samantha Rh2BG476100 Rh2DG484800 Rh6BG483600 Rh6DG474800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 57
AcuI CTGAAG 1 cut(s) 241
AfaI GTAC 1 cut(s) 254
AjnI CCWGG 1 cut(s) 287
AleI CACNNNNGTG 1 cut(s) 119
AluBI AGCT 3 cut(s) 31, 39, 208
AluI AGCT 3 cut(s) 31, 39, 208
AlwI GGATC 1 cut(s) 57
AoxI GGCC 1 cut(s) 244
ApaI GGGCCC 1 cut(s) 248
ApeKI GCWGC 1 cut(s) 160
AspA2I CCTAGG 1 cut(s) 88
AspLEI GCGC 1 cut(s) 98
AspS9I GGNCC 2 cut(s) 244, 245
AsuC2I CCSGG 1 cut(s) 243
AsuHPI GGTGA 1 cut(s) 106
AvrII CCTAGG 1 cut(s) 88
BaeGI GKGCMC 1 cut(s) 248
BanII GRGCYC 1 cut(s) 248
BbvI GCAGC 1 cut(s) 147
BciT130I CCWGG 1 cut(s) 289
BclI TGATCA 1 cut(s) 234
BcnI CCSGG 1 cut(s) 243
BfaI CTAG 2 cut(s) 44, 89
BfoI RGCGCY 1 cut(s) 99
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 1 cut(s) 161
BlnI CCTAGG 1 cut(s) 88
BlsI GCNGC 1 cut(s) 162
BmcAI AGTACT 1 cut(s) 254
Bme1390I CCNGG 2 cut(s) 243, 289
BmgT120I GGNCC 2 cut(s) 244, 245
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 2 cut(s) 243, 289
BmsI GCATC 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 17
BpuMI CCSGG 1 cut(s) 243
BsaJI CCNNGG 3 cut(s) 88, 117, 287
Bse3DI GCAATG 1 cut(s) 280
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 3 cut(s) 88, 117, 287
BseMI GCAATG 1 cut(s) 280
BseSI GKGCMC 1 cut(s) 248
BseXI GCAGC 1 cut(s) 147
BseYI CCCAGC 1 cut(s) 208
BshFI GGCC 1 cut(s) 246
BsiSI CCGG 1 cut(s) 242
BsnI GGCC 1 cut(s) 246
Bsp120I GGGCCC 1 cut(s) 244
Bsp1286I GDGCHC 1 cut(s) 248
Bsp143I GATC 2 cut(s) 49, 234
BspANI GGCC 1 cut(s) 246
BspLI GGNNCC 1 cut(s) 246
BspPI GGATC 1 cut(s) 57
BsrDI GCAATG 1 cut(s) 280
BssECI CCNNGG 3 cut(s) 88, 117, 287
BssMI GATC 2 cut(s) 49, 234
BssT1I CCWWGG 1 cut(s) 88
Bst2UI CCWGG 1 cut(s) 289
BstAPI GCANNNNNTGC 1 cut(s) 160
BstC8I GCNNGC 1 cut(s) 206
BstH2I RGCGCY 1 cut(s) 99
BstHHI GCGC 1 cut(s) 98
BstKTI GATC 2 cut(s) 52, 237
BstMBI GATC 2 cut(s) 49, 234
BstMWI GCNNNNNNNGC 6 cut(s) 14, 68, 93, 130, 160, 292
BstNI CCWGG 1 cut(s) 289
BstSCI CCNGG 2 cut(s) 241, 287
BstSLI GKGCMC 1 cut(s) 248
BstV1I GCAGC 1 cut(s) 147
BsuRI GGCC 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 206
CfoI GCGC 1 cut(s) 98
Cfr13I GGNCC 2 cut(s) 244, 245
Csp6I GTAC 1 cut(s) 253
CviAII CATG 1 cut(s) 134
CviJI RGCY 6 cut(s) 31, 39, 87, 160, 208, 246
CviKI_1 RGCY 6 cut(s) 31, 39, 87, 160, 208, 246
CviQI GTAC 1 cut(s) 253
DpnI GATC 2 cut(s) 51, 236
DpnII GATC 2 cut(s) 49, 234
Eco130I CCWWGG 1 cut(s) 88
Eco24I GRGCYC 1 cut(s) 248
Eco57I CTGAAG 1 cut(s) 241
EcoRII CCWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 88
EcoT38I GRGCYC 1 cut(s) 248
ErhI CCWWGG 1 cut(s) 88
FaeI CATG 1 cut(s) 137
FaiI YATR 7 cut(s) 10, 54, 84, 135, 300, 321, 346
FatI CATG 1 cut(s) 133
FbaI TGATCA 1 cut(s) 234
Fnu4HI GCNGC 1 cut(s) 161
FriOI GRGCYC 1 cut(s) 248
Fsp4HI GCNGC 1 cut(s) 161
FspBI CTAG 2 cut(s) 44, 89
GlaI GCGC 1 cut(s) 97
GluI GCNGC 1 cut(s) 161
GsaI CCCAGC 1 cut(s) 212
HaeII RGCGCY 1 cut(s) 99
HaeIII GGCC 1 cut(s) 246
HapII CCGG 1 cut(s) 242
HhaI GCGC 1 cut(s) 98
Hin1II CATG 1 cut(s) 137
Hin6I GCGC 1 cut(s) 96
HinP1I GCGC 1 cut(s) 96
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HpaII CCGG 1 cut(s) 242
HphI GGTGA 1 cut(s) 106
Hpy166II GTNNAC 3 cut(s) 114, 172, 268
Hpy188III TCNNGA 1 cut(s) 34
Hpy8I GTNNAC 3 cut(s) 114, 172, 268
HpyCH4V TGCA 6 cut(s) 8, 62, 133, 154, 182, 273
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 68, 93, 130, 160, 292
Hsp92II CATG 1 cut(s) 137
HspAI GCGC 1 cut(s) 96
Ksp22I TGATCA 1 cut(s) 234
Kzo9I GATC 2 cut(s) 49, 234
LpnPI CCDG 7 cut(s) 110, 194, 218, 244, 255, 274, 301
Lsp1109I GCAGC 1 cut(s) 147
LweI GCATC 1 cut(s) 141
MaeI CTAG 2 cut(s) 44, 89
MaeIII GTNAC 1 cut(s) 73
MalI GATC 2 cut(s) 51, 236
MboI GATC 2 cut(s) 49, 234
MhlI GDGCHC 1 cut(s) 248
MnlI CCTC 5 cut(s) 40, 115, 127, 207, 217
MslI CAYNNNNRTG 2 cut(s) 57, 119
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 2 cut(s) 243, 289
MvaI CCWGG 1 cut(s) 289
MwoI GCNNNNNNNGC 6 cut(s) 14, 68, 93, 130, 160, 292
NciI CCSGG 1 cut(s) 243
NdeII GATC 2 cut(s) 49, 234
NlaIII CATG 1 cut(s) 137
NlaIV GGNNCC 1 cut(s) 246
NmuCI GTSAC 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 119
PkrI GCNGC 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 287
PspFI CCCAGC 1 cut(s) 208
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 1 cut(s) 246
PspOMI GGGCCC 1 cut(s) 244
PspPI GGNCC 2 cut(s) 244, 245
PvuII CAGCTG 1 cut(s) 208
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
RseI CAYNNNNRTG 2 cut(s) 57, 119
SatI GCNGC 1 cut(s) 161
Sau3AI GATC 2 cut(s) 49, 234
Sau96I GGNCC 2 cut(s) 244, 245
ScaI AGTACT 1 cut(s) 254
ScrFI CCNGG 2 cut(s) 243, 289
SduI GDGCHC 1 cut(s) 248
SetI ASST 7 cut(s) 33, 41, 94, 119, 210, 218, 293
SfaNI GCATC 1 cut(s) 141
SmiMI CAYNNNNRTG 2 cut(s) 57, 119
SmlI CTYRAG 1 cut(s) 32
SmoI CTYRAG 1 cut(s) 32
SspMI CTAG 2 cut(s) 44, 89
StyD4I CCNGG 2 cut(s) 241, 287
StyI CCWWGG 1 cut(s) 88
TatI WGTACW 1 cut(s) 252
TseFI GTSAC 1 cut(s) 73
TseI GCWGC 1 cut(s) 160
Tsp45I GTSAC 1 cut(s) 73
TspDTI ATGAA 2 cut(s) 158, 318
XmaJI CCTAGG 1 cut(s) 88
XspI CTAG 2 cut(s) 44, 89
ZrmI AGTACT 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.