FvH4_6g37811

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017343
Physical Location & Seq
Reverse (-)
29769534 .. 29771571
2038 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37811.t1

Sequence Viewer

Length: 1641 bp
ATGGACAGAGCTTTTGAATGTTGTGTGTTGAACGGCTTTAGTGTTGTAGTGTTGTACAGCCAAAACCTTGAGAACCCAGAAAGTTTAGGGGATATGATTCTAGGTAAGAAGCTCTTAGCTTTAGTATGGTCTGCACACAGAAATTTCAGCACAAAAGTTAGTTACAAAAGCAAGAAATGGCCTCAGGATAGATATAAGACCAGAGACAAACCCAAGAAGGGAGAAGTTGAGAACACAAAAACCAAAATTCAATGGACTGATGAACAGAAACAAATCATATCTGCCATTTCTGAGGGAAAGTCTGTGTTCATTACCGGTTCGGCGGGCACTGGAAAAACAATTTTGGTTCAGCATGTTATCAAATTGTTAAAAAGGCAGCATAGAGCCTCTAAGGTTTTTGTCACAGCGCCTACTGGTGTTGCGGCTTGTGCAATTGGGGGGCAGACTCTTCATTGTTTCGCGGGGATTGGGTATCCCATGGCTGATAGGGAGACTTTGCTGCATAGGATTGTCCTAAATAACAAGGCTTACAACAGGTGGTTGAAAGTTAAGGCATTGGTTATCGATGAGATTAGCATGGTGGATACTGAGTTTTTCGAGAGTCTTGAATACATTGCCAGAAATATACGACAAGTCAATAAAAGTTGGGGTGGGATTCAGATTGTTGTGGGTGGGGATTTCTTTCAGTTACCTCCCATTAAACCCCAGCAGAATTCAGGGGGAAGAGAATTTGCTTTTGAAGCCGATTGTTGGAATTCTAGCTTTGACCTGCAGGTAAGTCTTACCAAAGTGTTTCGCCAATCAGATCCTCGGCTTATCAAGTTACTTAATGGGATAAGAAGAGGAGAGAGTGACCCTGAAGACTTGATGTTTCTTGAACAATCTTGCTCAGAGGCTGAACCAGATCCTTCTGTGGTACAACTCTATCCAAGGATTGAAGATGTGAATAGAGTGAATGCAAGTAGATTGGTAAGCTTGGGCAATGAATTTGTTATCTATACAGCTATTGATAGTGGTGAGGAGTCTAGGAAATCACAGCTTGAACAGGGAATAGCCCCCAAGGAAGTTACATTGTGCAAAGATGCGAGGGTGATGCTGGTCAAGAATTTGAATACTTGGCGTGGCCTGGTGAATGGTGCTACTGGTACGGTTCAGGGATTTTATGTGCCCAAAAATAAGGATATAGCTGGCGTATGCCATGCTCTACCAATAGTCAAGTTTGATTCAGGGCCAGAAATGTTGATTGAACCAGAAACATGGGCTGTGACAGAGGGAGATAAAGTTGTTGCTGAACGAAAGCAGGTGCCTCTCATATTAGCATGGGCTTCAAGCATTCACAAGTGCCAAGGCATGACTCTTGACTGCCTTCACACTGATCTCTCTAAAGCTTTCGAGAATGGAATGGTTTATGTAGCTCTTTCCCGTGTGAGAAGCTTGGAAGGCCTGTATTTATCTGGTTTCAATCGCTGGAAGATTATGGTTCACCCTAAAGTTTTGCTGTTCTATAAAAGTTTTAGTGGTGAAGTGGCTCAGGAGGAAAATGATGACAATGATAGCCAGGGACAAGATAGCATGAGCCGCATTGGTAATGGCAGCCAGGAAGATGATGGCAGCAAATACATGCTGGTTCACATAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PIF1

Protein Analysis

547

Amino Acids

61.14

Weight (kDa)

8.18

Isoelectric Point (pI)

37.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIF1 PF05970 86 - 270 1.8e-42 PIF1-like helicase
AAA_30 PF13604 86 - 293 3.9e-15 AAA domain
DEAD PF00270 86 - 212 5.3e-07 DEAD/DEAH box helicase
AAA_19 PF13245 89 - 233 2.5e-15 AAA domain
AAA_22 PF13401 99 - 222 5.9e-07 AAA domain
Pif1_2B_dom PF21530 349 - 387 4.4e-07 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017343)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g37811
prunus_persica Prupe.3G170200_v2.0.a1
pyrus_communis pycom09g07450 pycom17g13510
rosa_laevigata RLG00000020517
rosa_multiflora Rmu_sc0000185.1_g000025
rosa_roxburghii Rroxscaffold_2G00096840
rosa_rugosa Rorug02G0423300
rosa_samantha Rh2AG483700 Rh2BG496000 Rh2CG469900 Rh2DG507000
rosa_wichuraiana Rw2G039640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1291
Acc36I ACCTGC 3 cut(s) 763, 777, 1291
AccB1I GGYRCC 1 cut(s) 1303
AccII CGCG 1 cut(s) 461
AciI CCGC 4 cut(s) 323, 422, 461, 1579
AclWI GGATC 2 cut(s) 800, 899
AcsI RAATTY 7 cut(s) 142, 246, 712, 728, 754, 986, 1105
AcuI CTGAAG 1 cut(s) 879
AfaI GTAC 3 cut(s) 56, 918, 1147
AfiI CCNNNNNNNGG 2 cut(s) 218, 750
AgeI ACCGGT 1 cut(s) 314
AjnI CCWGG 3 cut(s) 1125, 1557, 1596
Alw26I GTCTC 2 cut(s) 198, 485
AlwI GGATC 2 cut(s) 800, 899
AlwNI CAGNNNCTG 1 cut(s) 896
AoxI GGCC 4 cut(s) 179, 1123, 1229, 1441
ApeKI GCWGC 4 cut(s) 376, 499, 1593, 1611
ApoI RAATTY 7 cut(s) 142, 246, 712, 728, 754, 986, 1105
ArsI GACNNNNNNTTYG 1 cut(s) 28
AsiGI ACCGGT 1 cut(s) 314
AspLEI GCGC 1 cut(s) 409
AspS9I GGNCC 1 cut(s) 1229
AsuHPI GGTGA 5 cut(s) 1028, 1102, 1141, 1475, 1532
AxyI CCTNAGG 1 cut(s) 183
BaeGI GKGCMC 2 cut(s) 329, 1170
BanI GGYRCC 1 cut(s) 1303
BbsI GAAGAC 1 cut(s) 867
BbvI GCAGC 4 cut(s) 388, 486, 1605, 1623
BccI CCATC 1 cut(s) 1601
BceAI ACGGC 1 cut(s) 49
BcgI CGANNNNNNTGC 2 cut(s) 1075, 1109
BciT130I CCWGG 3 cut(s) 1127, 1559, 1598
BciVI GTATCC 2 cut(s) 483, 577
BcoDI GTCTC 2 cut(s) 198, 485
BfaI CTAG 3 cut(s) 101, 759, 1026
BfmI CTRYAG 1 cut(s) 770
BfoI RGCGCY 1 cut(s) 410
BfuAI ACCTGC 3 cut(s) 763, 777, 1291
BfuI GTATCC 2 cut(s) 483, 577
BisI GCNGC 6 cut(s) 377, 423, 500, 1579, 1594, 1612
BlsI GCNGC 6 cut(s) 378, 424, 501, 1580, 1595, 1613
Bme1390I CCNGG 3 cut(s) 1127, 1559, 1598
BmgT120I GGNCC 1 cut(s) 1229
BmiI GGNNCC 1 cut(s) 1305
BmrFI CCNGG 3 cut(s) 1127, 1559, 1598
BmsI GCATC 2 cut(s) 1072, 1083
BpiI GAAGAC 1 cut(s) 867
Bpu10I CCTNAGC 1 cut(s) 1530
BpuEI CTTGAG 1 cut(s) 89
Bsa29I ATCGAT 1 cut(s) 564
BsaJI CCNNGG 6 cut(s) 477, 809, 929, 1059, 1345, 1558
BsaWI WCCGGW 1 cut(s) 314
BsaXI ACNNNNNCTCC 2 cut(s) 1266, 1296
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 750
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 3 cut(s) 334, 418, 1147
Bse21I CCTNAGG 1 cut(s) 183
Bse3DI GCAATG 2 cut(s) 612, 988
BseBI CCWGG 3 cut(s) 1127, 1559, 1598
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 6 cut(s) 477, 809, 929, 1059, 1345, 1558
BseLI CCNNNNNNNGG 2 cut(s) 218, 750
BseMI GCAATG 2 cut(s) 612, 988
BseMII CTCAG 5 cut(s) 197, 282, 579, 903, 1544
BseNI ACTGG 3 cut(s) 334, 418, 1147
BseRI GAGGAG 2 cut(s) 858, 1034
BseSI GKGCMC 2 cut(s) 329, 1170
BseXI GCAGC 4 cut(s) 388, 486, 1605, 1623
BseYI CCCAGC 1 cut(s) 705
BsgI GTGCAG 1 cut(s) 117
Bsh1236I CGCG 1 cut(s) 461
BshFI GGCC 4 cut(s) 181, 1125, 1231, 1443
BshNI GGYRCC 1 cut(s) 1303
BshTI ACCGGT 1 cut(s) 314
BshVI ATCGAT 1 cut(s) 564
BsiSI CCGG 1 cut(s) 315
BslFI GGGAC 1 cut(s) 1575
BslI CCNNNNNNNGG 2 cut(s) 218, 750
BsmAI GTCTC 2 cut(s) 198, 485
BsmFI GGGAC 1 cut(s) 1575
BsmI GAATGC 2 cut(s) 961, 1332
BsnI GGCC 4 cut(s) 181, 1125, 1231, 1443
Bsp1286I GDGCHC 2 cut(s) 329, 1170
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 3 cut(s) 805, 904, 1375
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 4 cut(s) 323, 422, 461, 1579
BspANI GGCC 4 cut(s) 181, 1125, 1231, 1443
BspCNI CTCAG 5 cut(s) 196, 283, 580, 902, 1543
BspDI ATCGAT 1 cut(s) 564
BspFNI CGCG 1 cut(s) 461
BspLI GGNNCC 1 cut(s) 1305
BspMAI CTGCAG 1 cut(s) 774
BspMI ACCTGC 3 cut(s) 763, 777, 1291
BspPI GGATC 2 cut(s) 800, 899
BspT107I GGYRCC 1 cut(s) 1303
BsrDI GCAATG 2 cut(s) 612, 988
BsrFI RCCGGY 1 cut(s) 314
BsrGI TGTACA 1 cut(s) 54
BsrI ACTGG 3 cut(s) 334, 418, 1147
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 6 cut(s) 477, 809, 929, 1059, 1345, 1558
BssMI GATC 3 cut(s) 805, 904, 1375
BssT1I CCWWGG 4 cut(s) 477, 929, 1059, 1345
Bst2UI CCWGG 3 cut(s) 1127, 1559, 1598
Bst4CI ACNGT 1 cut(s) 1150
Bst6I CTCTTC 3 cut(s) 453, 718, 835
BstAUI TGTACA 1 cut(s) 54
BstC8I GCNNGC 2 cut(s) 325, 1189
BstDEI CTNAG 7 cut(s) 115, 183, 291, 390, 588, 889, 1530
BstDSI CCRYGG 1 cut(s) 477
BstFNI CGCG 1 cut(s) 461
BstH2I RGCGCY 1 cut(s) 410
BstHHI GCGC 1 cut(s) 409
BstKTI GATC 3 cut(s) 808, 907, 1378
BstMAI GTCTC 2 cut(s) 198, 485
BstMBI GATC 3 cut(s) 805, 904, 1375
BstMWI GCNNNNNNNGC 4 cut(s) 428, 740, 1440, 1578
BstNI CCWGG 3 cut(s) 1127, 1559, 1598
BstNSI RCATGY 2 cut(s) 356, 1624
BstSCI CCNGG 3 cut(s) 1125, 1557, 1596
BstSFI CTRYAG 1 cut(s) 770
BstSLI GKGCMC 2 cut(s) 329, 1170
BstUI CGCG 1 cut(s) 461
BstV1I GCAGC 4 cut(s) 388, 486, 1605, 1623
BstV2I GAAGAC 1 cut(s) 867
BstX2I RGATCY 2 cut(s) 805, 904
BstXI CCANNNNNNTGG 1 cut(s) 1257
BstYI RGATCY 2 cut(s) 805, 904
Bsu15I ATCGAT 1 cut(s) 564
Bsu36I CCTNAGG 1 cut(s) 183
BsuI GTATCC 2 cut(s) 483, 577
BsuRI GGCC 4 cut(s) 181, 1125, 1231, 1443
BsuTUI ATCGAT 1 cut(s) 564
BtgI CCRYGG 1 cut(s) 477
BtsIMutI CAGTG 2 cut(s) 327, 1371
BveI ACCTGC 3 cut(s) 763, 777, 1291
Cac8I GCNNGC 2 cut(s) 325, 1189
CaiI CAGNNNCTG 1 cut(s) 896
CfoI GCGC 1 cut(s) 409
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 1229
ClaI ATCGAT 1 cut(s) 564
Csp6I GTAC 3 cut(s) 55, 917, 1146
CspAI ACCGGT 1 cut(s) 314
CviAII CATG 9 cut(s) 353, 478, 577, 1199, 1257, 1320, 1351, 1573, 1621
CviQI GTAC 3 cut(s) 55, 917, 1146
DdeI CTNAG 7 cut(s) 115, 183, 291, 390, 588, 889, 1530
DpnI GATC 3 cut(s) 807, 906, 1377
DpnII GATC 3 cut(s) 805, 904, 1375
Eam1104I CTCTTC 3 cut(s) 453, 718, 835
EarI CTCTTC 3 cut(s) 453, 718, 835
Eco130I CCWWGG 4 cut(s) 477, 929, 1059, 1345
Eco147I AGGCCT 1 cut(s) 1443
Eco57I CTGAAG 1 cut(s) 879
Eco81I CCTNAGG 1 cut(s) 183
EcoRI GAATTC 2 cut(s) 712, 754
EcoRII CCWGG 3 cut(s) 1125, 1557, 1596
EcoT14I CCWWGG 4 cut(s) 477, 929, 1059, 1345
ErhI CCWWGG 4 cut(s) 477, 929, 1059, 1345
FaeI CATG 9 cut(s) 356, 481, 580, 1202, 1260, 1323, 1354, 1576, 1624
FalI AAGNNNNNCTT 2 cut(s) 98, 130
FaqI GGGAC 1 cut(s) 1575
FatI CATG 9 cut(s) 352, 477, 576, 1198, 1256, 1319, 1350, 1572, 1620
FauI CCCGC 2 cut(s) 316, 454
Fnu4HI GCNGC 6 cut(s) 377, 423, 500, 1579, 1594, 1612
Fsp4HI GCNGC 6 cut(s) 377, 423, 500, 1579, 1594, 1612
FspBI CTAG 3 cut(s) 101, 759, 1026
GlaI GCGC 1 cut(s) 408
GluI GCNGC 6 cut(s) 377, 423, 500, 1579, 1594, 1612
GsaI CCCAGC 1 cut(s) 709
HaeII RGCGCY 1 cut(s) 410
HaeIII GGCC 4 cut(s) 181, 1125, 1231, 1443
HapII CCGG 1 cut(s) 315
HhaI GCGC 1 cut(s) 409
Hin1II CATG 9 cut(s) 356, 481, 580, 1202, 1260, 1323, 1354, 1576, 1624
Hin6I GCGC 1 cut(s) 407
HinP1I GCGC 1 cut(s) 407
HindIII AAGCTT 3 cut(s) 973, 1386, 1432
HinfI GANTC 7 cut(s) 97, 445, 601, 655, 1022, 1223, 1354
HpaII CCGG 1 cut(s) 315
HphI GGTGA 5 cut(s) 1028, 1102, 1141, 1475, 1532
Hpy166II GTNNAC 2 cut(s) 1483, 1630
Hpy188I TCNGA 4 cut(s) 292, 660, 805, 892
Hpy188III TCNNGA 8 cut(s) 185, 598, 605, 875, 1102, 1358, 1393, 1532
Hpy8I GTNNAC 2 cut(s) 1483, 1630
HpyAV CCTTC 4 cut(s) 211, 918, 1376, 1433
HpyCH4III ACNGT 1 cut(s) 1150
HpyCH4V TGCA 6 cut(s) 134, 431, 502, 772, 959, 1077
HpyF10VI GCNNNNNNNGC 4 cut(s) 428, 740, 1440, 1578
HpyF3I CTNAG 7 cut(s) 115, 183, 291, 390, 588, 889, 1530
Hsp92II CATG 9 cut(s) 356, 481, 580, 1202, 1260, 1323, 1354, 1576, 1624
HspAI GCGC 1 cut(s) 407
Kzo9I GATC 3 cut(s) 805, 904, 1375
Lsp1109I GCAGC 4 cut(s) 388, 486, 1605, 1623
LweI GCATC 2 cut(s) 1072, 1083
MaeI CTAG 3 cut(s) 101, 759, 1026
MaeIII GTNAC 7 cut(s) 161, 400, 687, 822, 851, 1066, 1264
MalI GATC 3 cut(s) 807, 906, 1377
MboI GATC 3 cut(s) 805, 904, 1375
MboII GAAGA 7 cut(s) 440, 735, 852, 872, 950, 1483, 1613
MfeI CAATTG 1 cut(s) 432
MflI RGATCY 2 cut(s) 805, 904
MhlI GDGCHC 2 cut(s) 329, 1170
MlyI GAGTC 4 cut(s) 439, 610, 1031, 1348
MmeI TCCRAC 1 cut(s) 731
MseI TTAA 4 cut(s) 368, 549, 699, 828
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 3 cut(s) 1127, 1559, 1598
MunI CAATTG 1 cut(s) 432
Mva1269I GAATGC 2 cut(s) 961, 1332
MvaI CCWGG 3 cut(s) 1127, 1559, 1598
MvnI CGCG 1 cut(s) 461
MwoI GCNNNNNNNGC 4 cut(s) 428, 740, 1440, 1578
NcoI CCATGG 1 cut(s) 477
NdeII GATC 3 cut(s) 805, 904, 1375
NlaIII CATG 9 cut(s) 356, 481, 580, 1202, 1260, 1323, 1354, 1576, 1624
NlaIV GGNNCC 1 cut(s) 1305
NmeAIII GCCGAG 1 cut(s) 790
NmuCI GTSAC 3 cut(s) 400, 851, 1264
NspI RCATGY 2 cut(s) 356, 1624
PaqCI CACCTGC 1 cut(s) 1291
PceI AGGCCT 1 cut(s) 1443
PctI GAATGC 2 cut(s) 961, 1332
PfeI GAWTC 3 cut(s) 97, 655, 1223
PinAI ACCGGT 1 cut(s) 314
PkrI GCNGC 6 cut(s) 378, 424, 501, 1580, 1595, 1613
PleI GAGTC 4 cut(s) 439, 609, 1030, 1348
PpsI GAGTC 4 cut(s) 439, 609, 1030, 1348
Psp6I CCWGG 3 cut(s) 1125, 1557, 1596
PspFI CCCAGC 1 cut(s) 705
PspGI CCWGG 3 cut(s) 1125, 1557, 1596
PspN4I GGNNCC 1 cut(s) 1305
PspPI GGNCC 1 cut(s) 1229
PstI CTGCAG 1 cut(s) 774
PstNI CAGNNNCTG 1 cut(s) 896
PsuI RGATCY 2 cut(s) 805, 904
RsaI GTAC 3 cut(s) 56, 918, 1147
RsaNI GTAC 3 cut(s) 55, 917, 1146
SaqAI TTAA 4 cut(s) 368, 549, 699, 828
SatI GCNGC 6 cut(s) 377, 423, 500, 1579, 1594, 1612
Sau3AI GATC 3 cut(s) 805, 904, 1375
Sau96I GGNCC 1 cut(s) 1229
SbfI CCTGCAGG 1 cut(s) 774
SchI GAGTC 4 cut(s) 439, 610, 1031, 1348
ScrFI CCNGG 3 cut(s) 1127, 1559, 1598
SdaI CCTGCAGG 1 cut(s) 774
SduI GDGCHC 2 cut(s) 329, 1170
SfaNI GCATC 2 cut(s) 1072, 1083
SfcI CTRYAG 1 cut(s) 770
SmlI CTYRAG 1 cut(s) 68
SmoI CTYRAG 1 cut(s) 68
Sse8387I CCTGCAGG 1 cut(s) 774
SseBI AGGCCT 1 cut(s) 1443
SsiI CCGC 4 cut(s) 323, 422, 461, 1579
SspMI CTAG 3 cut(s) 101, 759, 1026
StuI AGGCCT 1 cut(s) 1443
StyD4I CCNGG 3 cut(s) 1125, 1557, 1596
StyI CCWWGG 4 cut(s) 477, 929, 1059, 1345
TaaI ACNGT 1 cut(s) 1150
TaqI TCGA 3 cut(s) 564, 597, 1392
TatI WGTACW 1 cut(s) 54
TauI GCSGC 2 cut(s) 425, 1581
TfiI GAWTC 3 cut(s) 97, 655, 1223
Tru1I TTAA 4 cut(s) 368, 549, 699, 828
Tru9I TTAA 4 cut(s) 368, 549, 699, 828
TscAI CASTG 2 cut(s) 334, 1378
TseFI GTSAC 3 cut(s) 400, 851, 1264
TseI GCWGC 4 cut(s) 376, 499, 1593, 1611
Tsp45I GTSAC 3 cut(s) 400, 851, 1264
TspDTI ATGAA 4 cut(s) 276, 298, 440, 999
TspRI CASTG 2 cut(s) 334, 1378
XapI RAATTY 7 cut(s) 142, 246, 712, 728, 754, 986, 1105
XceI RCATGY 2 cut(s) 356, 1624
XcmI CCANNNNNNNNNTGG 1 cut(s) 1604
XspI CTAG 3 cut(s) 101, 759, 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.