Rorug02G0423300

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0017343
Physical Location & Seq
Forward (+)
54430623 .. 54439199
8577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0423300.1

Sequence Viewer

Length: 831 bp
ATGACGACGACGAACACAGTGAGGCAGAGAGCTCTTCTTCTCCGCGATGTTCTGTCGCGCAGGGACATGGATGCCAGGCTCACCTTTCTGCAGAATTCGAGCACGAGCTCGCTCCGAATCCGCGAGAAGCTGTGTGCGTTGTGCGTACCAGTGGTTGGTCTCATTCAGGCTTTCATCTTTAGCTTAGTTGGGTGCTTCGATCACCACCTTCCACGTCATCAACACCGAGCCGCTTTCACCCACGACGACGTCGTTCGCCTCGCTGCCACCTCCCCATTTAGTGTAAATGAGGTGGAAGCATTGCGTGAATTGTTCAACAAGTTGAGTAGTTCGATTCTAGATGATGGCTTAATTCACAAGGAGGAGCTGCAGTTGCCTCTGTTCAAAACTCATGCGGGCGAAAATCTTTTTCTTGATAGGGTATTTGATGTTTTTGACGAAAAGAAAAATGGAGTCATTGAATTTGATGAGTTTATCCATGCATTAAGTGTATTTCATCCCTGCACTCCTCTTGAACAGAAGATTGACTTTGCATTCAGGTTGTATGATTTGAGACAGACTGGATATATTGAGCGGGAAGAAGTTAGGCAAATGGTAGTCGCCACCTTGTTGGAATCTGGCATACAGGTGCCGGATGAAACTCTAGAAACAATTGTGGATAAGACATTTGAAGATGCTGATGCTGATAAGGATGGCAAAATTAGCAACGATGAATGGAAGGCTTTTGTATTTCAGCGGCCTACACTCGTAAAGAACATGACACTTCCATATTTAAAGGACATCACCACAGTCTTCCCAAGTTTTATTTTCAACACTGAAGTCGAGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PIF1

Protein Analysis

276

Amino Acids

31.74

Weight (kDa)

5.17

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 172 - 238 2.7e-10 EF-hand domain pair
EF-hand_5 PF13202 219 - 243 2.8e-07 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017343)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g37811
prunus_persica Prupe.3G170200_v2.0.a1
pyrus_communis pycom09g07450 pycom17g13510
rosa_laevigata RLG00000020517
rosa_multiflora Rmu_sc0000185.1_g000025
rosa_roxburghii Rroxscaffold_2G00096840
rosa_rugosa Rorug02G0423300
rosa_samantha Rh2AG483700 Rh2BG496000 Rh2CG469900 Rh2DG507000
rosa_wichuraiana Rw2G039640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 252
AccB1I GGYRCC 1 cut(s) 628
AccB7I CCANNNNNTGG 1 cut(s) 155
AccBSI CCGCTC 1 cut(s) 574
AccII CGCG 3 cut(s) 45, 58, 123
AciI CCGC 6 cut(s) 43, 121, 231, 395, 574, 736
AcsI RAATTY 2 cut(s) 94, 461
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 1 cut(s) 155
AgsI TTSAA 6 cut(s) 316, 385, 461, 515, 671, 811
AjiI CACGTC 1 cut(s) 215
AjnI CCWGG 1 cut(s) 74
AluBI AGCT 5 cut(s) 32, 108, 130, 183, 367
AluI AGCT 5 cut(s) 32, 108, 130, 183, 367
Alw21I GWGCWC 3 cut(s) 34, 104, 110
Alw26I GTCTC 2 cut(s) 164, 547
AoxI GGCC 1 cut(s) 737
ApeKI GCWGC 2 cut(s) 263, 367
ApoI RAATTY 2 cut(s) 94, 461
AspLEI GCGC 1 cut(s) 60
AsuHPI GGTGA 4 cut(s) 73, 194, 229, 775
BanI GGYRCC 1 cut(s) 628
BanII GRGCYC 2 cut(s) 34, 110
BauI CACGAG 1 cut(s) 103
BbsI GAAGAC 1 cut(s) 784
Bbv12I GWGCWC 3 cut(s) 34, 104, 110
BbvI GCAGC 2 cut(s) 250, 354
BccI CCATC 2 cut(s) 338, 686
BciT130I CCWGG 1 cut(s) 76
BcoDI GTCTC 2 cut(s) 164, 547
BfaI CTAG 2 cut(s) 338, 644
BfmI CTRYAG 2 cut(s) 89, 368
BisI GCNGC 4 cut(s) 231, 264, 368, 737
BlsI GCNGC 4 cut(s) 232, 265, 369, 738
Bme1390I CCNGG 1 cut(s) 76
BmgBI CACGTC 1 cut(s) 215
BmiI GGNNCC 1 cut(s) 630
BmrFI CCNGG 1 cut(s) 76
BmsI GCATC 3 cut(s) 61, 664, 670
BpiI GAAGAC 1 cut(s) 784
BsaHI GRCGYC 1 cut(s) 249
BsaI GGTCTC 1 cut(s) 164
BsaXI ACNNNNNCTCC 2 cut(s) 356, 386
Bsc4I CCNNNNNNNGG 1 cut(s) 155
Bse1I ACTGG 2 cut(s) 149, 565
Bse3DI GCAATG 1 cut(s) 299
BseBI CCWGG 1 cut(s) 76
BseGI GGATG 4 cut(s) 76, 496, 640, 697
BseLI CCNNNNNNNGG 1 cut(s) 155
BseMI GCAATG 1 cut(s) 299
BseNI ACTGG 2 cut(s) 149, 565
BseRI GAGGAG 2 cut(s) 377, 498
BseXI GCAGC 2 cut(s) 250, 354
BsgI GTGCAG 1 cut(s) 487
Bsh1236I CGCG 3 cut(s) 45, 58, 123
BshFI GGCC 1 cut(s) 739
BshNI GGYRCC 1 cut(s) 628
BsiHKAI GWGCWC 3 cut(s) 34, 104, 110
BsiSI CCGG 1 cut(s) 632
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 164, 547
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 1 cut(s) 739
Bso31I GGTCTC 1 cut(s) 164
Bsp1286I GDGCHC 3 cut(s) 34, 104, 110
Bsp143I GATC 1 cut(s) 199
BspACI CCGC 6 cut(s) 43, 121, 231, 395, 574, 736
BspANI GGCC 1 cut(s) 739
BspFNI CGCG 3 cut(s) 45, 58, 123
BspLI GGNNCC 1 cut(s) 630
BspMAI CTGCAG 2 cut(s) 93, 372
BspQI GCTCTTC 1 cut(s) 39
BspT107I GGYRCC 1 cut(s) 628
BspTNI GGTCTC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 574
BsrDI GCAATG 1 cut(s) 299
BsrI ACTGG 2 cut(s) 149, 565
BssMI GATC 1 cut(s) 199
BssNI GRCGYC 1 cut(s) 249
BssSI CACGAG 1 cut(s) 103
Bst2BI CACGAG 1 cut(s) 103
Bst2UI CCWGG 1 cut(s) 76
Bst4CI ACNGT 2 cut(s) 19, 790
Bst6I CTCTTC 1 cut(s) 39
BstACI GRCGYC 1 cut(s) 249
BstC8I GCNNGC 2 cut(s) 110, 397
BstDEI CTNAG 1 cut(s) 184
BstF5I GGATG 4 cut(s) 76, 496, 640, 697
BstFNI CGCG 3 cut(s) 45, 58, 123
BstHHI GCGC 1 cut(s) 60
BstKTI GATC 1 cut(s) 202
BstMAI GTCTC 2 cut(s) 164, 547
BstMBI GATC 1 cut(s) 199
BstMWI GCNNNNNNNGC 2 cut(s) 373, 702
BstNI CCWGG 1 cut(s) 76
BstSCI CCNGG 1 cut(s) 74
BstSFI CTRYAG 2 cut(s) 89, 368
BstUI CGCG 3 cut(s) 45, 58, 123
BstV1I GCAGC 2 cut(s) 250, 354
BstV2I GAAGAC 1 cut(s) 784
BstXI CCANNNNNNTGG 1 cut(s) 610
BsuRI GGCC 1 cut(s) 739
BtgZI GCGATG 1 cut(s) 60
BtrI CACGTC 1 cut(s) 215
BtsCI GGATG 4 cut(s) 76, 496, 640, 697
BtsIMutI CAGTG 3 cut(s) 24, 156, 813
Cac8I GCNNGC 2 cut(s) 110, 397
CfoI GCGC 1 cut(s) 60
Csp6I GTAC 1 cut(s) 146
CviAII CATG 4 cut(s) 67, 392, 479, 757
CviQI GTAC 1 cut(s) 146
DdeI CTNAG 1 cut(s) 184
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
DraI TTTAAA 1 cut(s) 774
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Ecl136II GAGCTC 2 cut(s) 32, 108
Eco24I GRGCYC 2 cut(s) 34, 110
Eco31I GGTCTC 1 cut(s) 164
Eco53kI GAGCTC 2 cut(s) 32, 108
EcoICRI GAGCTC 2 cut(s) 32, 108
EcoRI GAATTC 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 74
EcoT22I ATGCAT 1 cut(s) 484
EcoT38I GRGCYC 2 cut(s) 34, 110
FaeI CATG 4 cut(s) 70, 395, 482, 760
FaiI YATR 8 cut(s) 68, 393, 480, 546, 567, 623, 758, 769
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FaqI GGGAC 1 cut(s) 77
FatI CATG 4 cut(s) 66, 391, 478, 756
FauI CCCGC 2 cut(s) 388, 567
Fnu4HI GCNGC 4 cut(s) 231, 264, 368, 737
FokI GGATG 4 cut(s) 83, 483, 647, 704
FriOI GRGCYC 2 cut(s) 34, 110
Fsp4HI GCNGC 4 cut(s) 231, 264, 368, 737
FspBI CTAG 2 cut(s) 338, 644
GlaI GCGC 1 cut(s) 59
GluI GCNGC 4 cut(s) 231, 264, 368, 737
HaeIII GGCC 1 cut(s) 739
HapII CCGG 1 cut(s) 632
HhaI GCGC 1 cut(s) 60
Hin1I GRCGYC 1 cut(s) 249
Hin1II CATG 4 cut(s) 70, 395, 482, 760
Hin6I GCGC 1 cut(s) 58
HinP1I GCGC 1 cut(s) 58
HinfI GANTC 4 cut(s) 117, 334, 453, 614
HpaII CCGG 1 cut(s) 632
HphI GGTGA 4 cut(s) 73, 194, 229, 775
Hpy188I TCNGA 1 cut(s) 116
Hpy188III TCNNGA 4 cut(s) 338, 413, 512, 644
Hpy99I CGWCG 5 cut(s) 10, 13, 248, 251, 254
HpyAV CCTTC 2 cut(s) 218, 712
HpyCH4III ACNGT 2 cut(s) 19, 790
HpyCH4IV ACGT 2 cut(s) 214, 249
HpyCH4V TGCA 5 cut(s) 91, 370, 482, 504, 533
HpyF10VI GCNNNNNNNGC 2 cut(s) 373, 702
HpyF3I CTNAG 1 cut(s) 184
HpySE526I ACGT 2 cut(s) 214, 249
Hsp92I GRCGYC 1 cut(s) 249
Hsp92II CATG 4 cut(s) 70, 395, 482, 760
HspAI GCGC 1 cut(s) 58
Kzo9I GATC 1 cut(s) 199
LguI GCTCTTC 1 cut(s) 39
LmnI GCTCC 2 cut(s) 117, 364
Lsp1109I GCAGC 2 cut(s) 250, 354
LweI GCATC 3 cut(s) 61, 664, 670
MaeI CTAG 2 cut(s) 338, 644
MaeII ACGT 2 cut(s) 214, 249
MalI GATC 1 cut(s) 201
MbiI CCGCTC 1 cut(s) 574
MboI GATC 1 cut(s) 199
MboII GAAGA 6 cut(s) 26, 29, 532, 590, 683, 784
MfeI CAATTG 1 cut(s) 651
MhlI GDGCHC 3 cut(s) 34, 104, 110
MluCI AATT 6 cut(s) 94, 308, 351, 461, 651, 699
MlyI GAGTC 1 cut(s) 462
MmeI TCCRAC 1 cut(s) 591
MnlI CCTC 8 cut(s) 15, 269, 280, 283, 355, 387, 519, 817
Mph1103I ATGCAT 1 cut(s) 484
MseI TTAA 3 cut(s) 350, 485, 773
MslI CAYNNNNRTG 1 cut(s) 626
MspA1I CMGCKG 1 cut(s) 736
MspI CCGG 1 cut(s) 632
MspR9I CCNGG 1 cut(s) 76
MunI CAATTG 1 cut(s) 651
Mva1269I GAATGC 1 cut(s) 533
MvaI CCWGG 1 cut(s) 76
MvnI CGCG 3 cut(s) 45, 58, 123
MwoI GCNNNNNNNGC 2 cut(s) 373, 702
NdeII GATC 1 cut(s) 199
NlaIII CATG 4 cut(s) 70, 395, 482, 760
NlaIV GGNNCC 1 cut(s) 630
NsiI ATGCAT 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 39
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PctI GAATGC 1 cut(s) 533
PfeI GAWTC 3 cut(s) 117, 334, 614
PflFI GACNNNGTC 1 cut(s) 248
PflMI CCANNNNNTGG 1 cut(s) 155
PkrI GCNGC 4 cut(s) 232, 265, 369, 738
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
Psp124BI GAGCTC 2 cut(s) 34, 110
Psp6I CCWGG 1 cut(s) 74
PspGI CCWGG 1 cut(s) 74
PspN4I GGNNCC 1 cut(s) 630
PstI CTGCAG 2 cut(s) 93, 372
PsyI GACNNNGTC 1 cut(s) 248
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
RseI CAYNNNNRTG 1 cut(s) 626
SacI GAGCTC 2 cut(s) 34, 110
SapI GCTCTTC 1 cut(s) 39
SaqAI TTAA 3 cut(s) 350, 485, 773
SatI GCNGC 4 cut(s) 231, 264, 368, 737
Sau3AI GATC 1 cut(s) 199
SchI GAGTC 1 cut(s) 462
ScrFI CCNGG 1 cut(s) 76
SduI GDGCHC 3 cut(s) 34, 104, 110
SfaNI GCATC 3 cut(s) 61, 664, 670
SfcI CTRYAG 2 cut(s) 89, 368
SmiMI CAYNNNNRTG 1 cut(s) 626
Sse9I AATT 6 cut(s) 94, 308, 351, 461, 651, 699
SsiI CCGC 6 cut(s) 43, 121, 231, 395, 574, 736
SspMI CTAG 2 cut(s) 338, 644
SstI GAGCTC 2 cut(s) 34, 110
StyD4I CCNGG 1 cut(s) 74
TaaI ACNGT 2 cut(s) 19, 790
TaiI ACGT 2 cut(s) 217, 252
TaqI TCGA 4 cut(s) 98, 198, 332, 822
TasI AATT 6 cut(s) 94, 308, 351, 461, 651, 699
TauI GCSGC 2 cut(s) 233, 739
TfiI GAWTC 3 cut(s) 117, 334, 614
Tru1I TTAA 3 cut(s) 350, 485, 773
Tru9I TTAA 3 cut(s) 350, 485, 773
TscAI CASTG 3 cut(s) 24, 156, 820
TseI GCWGC 2 cut(s) 263, 367
TspDTI ATGAA 4 cut(s) 163, 485, 651, 726
TspRI CASTG 3 cut(s) 24, 156, 820
Tth111I GACNNNGTC 1 cut(s) 248
Van91I CCANNNNNTGG 1 cut(s) 155
XapI RAATTY 2 cut(s) 94, 461
XbaI TCTAGA 2 cut(s) 337, 643
XspI CTAG 2 cut(s) 338, 644
ZraI GACGTC 1 cut(s) 250
Zsp2I ATGCAT 1 cut(s) 484
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.