Rh2AG483700

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0017343
Physical Location & Seq
Reverse (-)
70442047 .. 70443663
1617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG483700.1

Sequence Viewer

Length: 1617 bp
ATGAAGCTGTTAGCTTTAGTATTGTCTGCACACAGAAATTTCAGCACAAAAATTGGTTACAAAAGCAAGAAGTGGCCCCAGGATAGATACAAGACCAGATACAAACCCAAAAAGGGAGAAGTTGAGAACACAAAAACCAAAATTCAATGGACTGATGAACAAAAACAAGTCATATCTGCCATTTCTAAAGAAAAATCTGTGTTCATTACCGGTTCAGCGGGCACTGGAAAAACAATTTTGGTTCACCATATTATCAAATTGTTGAAAAAGCAGCATGGAGCCTCTAAGGTTTTTGTCACAGCGCCTACGGGTGTTGCAGCTTGTGCCATTGGGGGGCAGACTCTTCACTCTTTCGCGGGTATTGGGTATCCCATGGCTGATAGGGAGACCTTGCTGCATAGGATTGTCCTAAATAACAAGGCTTACAACAGGTGGTTGAAAGTTAAGGCTTTGGTTATGGATGAGATTAGCATGGTGGATACTGAGTTTTTCGAGAGTCTTGAATACATTGCCAGAAAAATACGAGAAGTCAATAAAAGTTGGGGTGGGATTCAGATTGTTGTGGGTGGGGATTTCTTTCAGTTACCTCCCATTAAACCCCAGCAGAATTCAGGGGGAAAAGAATATGCATTTGAAGCCGATTGTTGGAATTCTAGCTTTGACTTGCAGGTTAATCTGACAAAGGTGTTTCGCCAATCAGACCCTCAGCTTATCAAGTTACTTCATGGAATAAGAAGAGGAGAGAGTGATCCTGAAGACTTAAAGTTTCTTGAACAAGCTTGCTCGGAGGCTGAACCAGATCCTTCTGTGGTACAACTCTATCCAAGGATTGAAGATGTGAATAGAGTGAATGCAAGTAGATTAGAAAGCTTGGGCAATGAACTTTTTGTCTATACAGCTGTTGATAGTGGTGAGGAGTCTAGGAAACCACAGCTTGAACAGGGAATAGCCCCCAAGAAAGTTATTTTGTGTAAAGATGCGAGGGTGATGCTGGTCAAGAATTTGAATACTTGGCGTGGCCTGGTGAATGGTGCGACTGGTACAGTTCAGGGATTTTATGTGCCCAAAAATAAGGATATAACAGGCATATGCCATGCTGCTCTACCGATAGTCAAGTTTGATTCAGGGCCAGAACTGTTGATTGAACCAGAAACATGGGTTGTGACAGAGGGAGATAAGGTTGTTGCTGAACGGAAGCAGGTGCCTCTCATATTGGCATGGGCTTCAAGCATTCACAAGTGTCAGGGCATGACTCTTGACCGTCTTCACACTGATCTCTCTAAAGCTTTCGAGAATGGAATGGTTTATGTAGCTCTTTCCCGTGTGAGAAGCTTGGAAGGCCTGTATTTATCTGGTTTCGATCGCTGGAAGATTATGGTGCACCCTAAGGTATTGCAGTTCTATAAAGGTTTTAGTGGTGAACTGGGTCAGGAGGGCAAGGATGATAATGATAGCCAGAGACAAGATAGCATGAGCCGCAGTGGTAATGGCACTCAGAGACAGGATAGTACTAGCTGCAGTGGTAATGGCAGCCAGAGACAAAGTAGCACTAGGCGCAGTGGTAATGGCAACCAGAAAGATGATGGCAGCAAATACGTGCTGGTTCACATGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PIF1

Protein Analysis

538

Amino Acids

60.17

Weight (kDa)

9.3

Isoelectric Point (pI)

36.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ResIII PF04851 48 - 121 4.6e-06 Type III restriction enzyme, res subunit
PIF1 PF05970 51 - 235 1.2e-42 PIF1-like helicase
AAA_30 PF13604 51 - 259 8.3e-16 AAA domain
DEAD PF00270 51 - 177 2.8e-06 DEAD/DEAH box helicase
AAA_19 PF13245 54 - 198 1.2e-15 AAA domain
AAA_22 PF13401 63 - 187 2e-06 AAA domain
Pif1_2B_dom PF21530 315 - 352 1.8e-06 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017343)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g37811
prunus_persica Prupe.3G170200_v2.0.a1
pyrus_communis pycom09g07450 pycom17g13510
rosa_laevigata RLG00000020517
rosa_multiflora Rmu_sc0000185.1_g000025
rosa_roxburghii Rroxscaffold_2G00096840
rosa_rugosa Rorug02G0423300
rosa_samantha Rh2AG483700 Rh2BG496000 Rh2CG469900 Rh2DG507000
rosa_wichuraiana Rw2G039640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1189
Acc36I ACCTGC 2 cut(s) 658, 1189
AccB1I GGYRCC 1 cut(s) 1201
AccII CGCG 1 cut(s) 356
AciI CCGC 3 cut(s) 218, 356, 1477
AclWI GGATC 2 cut(s) 743, 794
AcsI RAATTY 5 cut(s) 37, 141, 607, 649, 1000
AcuI CTGAAG 1 cut(s) 774
AfaI GTAC 3 cut(s) 813, 1042, 1510
AfiI CCNNNNNNNGG 3 cut(s) 113, 333, 645
AgeI ACCGGT 1 cut(s) 209
AjnI CCWGG 2 cut(s) 78, 1020
Alw21I GWGCWC 1 cut(s) 1383
Alw26I GTCTC 4 cut(s) 380, 1453, 1492, 1531
Alw44I GTGCAC 1 cut(s) 1379
AlwI GGATC 2 cut(s) 743, 794
AoxI GGCC 4 cut(s) 74, 1018, 1127, 1339
ApaLI GTGCAC 1 cut(s) 1379
ApeKI GCWGC 7 cut(s) 271, 317, 394, 1097, 1515, 1530, 1587
ApoI RAATTY 5 cut(s) 37, 141, 607, 649, 1000
AsiGI ACCGGT 1 cut(s) 209
AspLEI GCGC 2 cut(s) 304, 1557
AspS9I GGNCC 2 cut(s) 75, 1127
AsuHPI GGTGA 5 cut(s) 236, 923, 997, 1036, 1430
AxyI CCTNAGG 1 cut(s) 1386
BaeGI GKGCMC 3 cut(s) 224, 1065, 1383
BanI GGYRCC 1 cut(s) 1201
BbsI GAAGAC 2 cut(s) 762, 1256
Bbv12I GWGCWC 1 cut(s) 1383
BbvCI CCTCAGC 1 cut(s) 705
BbvI GCAGC 7 cut(s) 283, 329, 381, 1084, 1502, 1542, 1599
BccI CCATC 1 cut(s) 1577
BcgI CGANNNNNNTGC 2 cut(s) 970, 1004
BciT130I CCWGG 2 cut(s) 80, 1022
BciVI GTATCC 2 cut(s) 378, 472
BcoDI GTCTC 4 cut(s) 380, 1453, 1492, 1531
BfaI CTAG 4 cut(s) 654, 921, 1512, 1551
BfmI CTRYAG 1 cut(s) 1516
BfoI RGCGCY 1 cut(s) 305
BfuAI ACCTGC 2 cut(s) 658, 1189
BfuI GTATCC 2 cut(s) 378, 472
BisI GCNGC 8 cut(s) 272, 318, 395, 1098, 1477, 1516, 1531, 1588
BlsI GCNGC 8 cut(s) 273, 319, 396, 1099, 1478, 1517, 1532, 1589
BmcAI AGTACT 1 cut(s) 1510
Bme1390I CCNGG 2 cut(s) 80, 1022
BmgT120I GGNCC 2 cut(s) 75, 1127
BmiI GGNNCC 3 cut(s) 77, 280, 1203
BmrFI CCNGG 2 cut(s) 80, 1022
BmrI ACTGGG 1 cut(s) 1433
BmsI GCATC 2 cut(s) 967, 978
BmuI ACTGGG 1 cut(s) 1433
BpiI GAAGAC 2 cut(s) 762, 1256
Bpu10I CCTNAGC 1 cut(s) 705
BsaAI YACGTR 1 cut(s) 1597
BsaI GGTCTC 1 cut(s) 380
BsaJI CCNNGG 3 cut(s) 78, 372, 824
BsaWI WCCGGW 1 cut(s) 209
BsaXI ACNNNNNCTCC 2 cut(s) 1164, 1194
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 333, 645
Bse118I RCCGGY 1 cut(s) 209
Bse1I ACTGG 3 cut(s) 229, 1042, 1428
Bse21I CCTNAGG 1 cut(s) 1386
Bse3DI GCAATG 2 cut(s) 507, 883
BseBI CCWGG 2 cut(s) 80, 1022
BseDI CCNNGG 3 cut(s) 78, 372, 824
BseGI GGATG 2 cut(s) 466, 1447
BseLI CCNNNNNNNGG 3 cut(s) 113, 333, 645
BseMI GCAATG 2 cut(s) 507, 883
BseMII CTCAG 3 cut(s) 474, 719, 1508
BseNI ACTGG 3 cut(s) 229, 1042, 1428
BseRI GAGGAG 2 cut(s) 753, 929
BseSI GKGCMC 3 cut(s) 224, 1065, 1383
BseXI GCAGC 7 cut(s) 283, 329, 381, 1084, 1502, 1542, 1599
BseYI CCCAGC 1 cut(s) 600
BsgI GTGCAG 1 cut(s) 12
Bsh1236I CGCG 1 cut(s) 356
Bsh1285I CGRYCG 1 cut(s) 1363
BshFI GGCC 4 cut(s) 76, 1020, 1129, 1341
BshNI GGYRCC 1 cut(s) 1201
BshTI ACCGGT 1 cut(s) 209
BsiEI CGRYCG 1 cut(s) 1363
BsiHKAI GWGCWC 1 cut(s) 1383
BsiSI CCGG 1 cut(s) 210
BslI CCNNNNNNNGG 3 cut(s) 113, 333, 645
BsmAI GTCTC 4 cut(s) 380, 1453, 1492, 1531
BsmI GAATGC 2 cut(s) 856, 1230
BsnI GGCC 4 cut(s) 76, 1020, 1129, 1341
Bso31I GGTCTC 1 cut(s) 380
Bsp1286I GDGCHC 3 cut(s) 224, 1065, 1383
Bsp143I GATC 4 cut(s) 748, 799, 1273, 1360
Bsp19I CCATGG 1 cut(s) 372
BspACI CCGC 3 cut(s) 218, 356, 1477
BspANI GGCC 4 cut(s) 76, 1020, 1129, 1341
BspCNI CTCAG 3 cut(s) 475, 718, 1507
BspFNI CGCG 1 cut(s) 356
BspLI GGNNCC 3 cut(s) 77, 280, 1203
BspMAI CTGCAG 1 cut(s) 1520
BspMI ACCTGC 2 cut(s) 658, 1189
BspPI GGATC 2 cut(s) 743, 794
BspT107I GGYRCC 1 cut(s) 1201
BspTNI GGTCTC 1 cut(s) 380
BsrDI GCAATG 2 cut(s) 507, 883
BsrFI RCCGGY 1 cut(s) 209
BsrI ACTGG 3 cut(s) 229, 1042, 1428
BssAI RCCGGY 1 cut(s) 209
BssECI CCNNGG 3 cut(s) 78, 372, 824
BssMI GATC 4 cut(s) 748, 799, 1273, 1360
BssT1I CCWWGG 2 cut(s) 372, 824
Bst2UI CCWGG 2 cut(s) 80, 1022
Bst4CI ACNGT 3 cut(s) 1045, 1137, 1262
Bst6I CTCTTC 2 cut(s) 348, 730
BstAPI GCANNNNNTGC 1 cut(s) 323
BstBAI YACGTR 1 cut(s) 1597
BstC8I GCNNGC 2 cut(s) 220, 781
BstDEI CTNAG 5 cut(s) 285, 483, 705, 1386, 1494
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 466, 1447
BstFNI CGCG 1 cut(s) 356
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 2 cut(s) 304, 1557
BstKTI GATC 4 cut(s) 751, 802, 1276, 1363
BstMAI GTCTC 4 cut(s) 380, 1453, 1492, 1531
BstMBI GATC 4 cut(s) 748, 799, 1273, 1360
BstMCI CGRYCG 1 cut(s) 1363
BstMWI GCNNNNNNNGC 5 cut(s) 323, 635, 1338, 1476, 1554
BstNI CCWGG 2 cut(s) 80, 1022
BstSCI CCNGG 2 cut(s) 78, 1020
BstSFI CTRYAG 1 cut(s) 1516
BstSLI GKGCMC 3 cut(s) 224, 1065, 1383
BstUI CGCG 1 cut(s) 356
BstV1I GCAGC 7 cut(s) 283, 329, 381, 1084, 1502, 1542, 1599
BstV2I GAAGAC 2 cut(s) 762, 1256
BstX2I RGATCY 1 cut(s) 799
BstXI CCANNNNNNTGG 1 cut(s) 1155
BstYI RGATCY 1 cut(s) 799
Bsu36I CCTNAGG 1 cut(s) 1386
BsuI GTATCC 2 cut(s) 378, 472
BsuRI GGCC 4 cut(s) 76, 1020, 1129, 1341
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 2 cut(s) 466, 1447
BtsI GCAGTG 3 cut(s) 1486, 1525, 1564
BtsIMutI CAGTG 5 cut(s) 222, 1269, 1486, 1525, 1564
BveI ACCTGC 2 cut(s) 658, 1189
Cac8I GCNNGC 2 cut(s) 220, 781
CfoI GCGC 2 cut(s) 304, 1557
Cfr10I RCCGGY 1 cut(s) 209
Cfr13I GGNCC 2 cut(s) 75, 1127
Csp6I GTAC 3 cut(s) 812, 1041, 1509
CspAI ACCGGT 1 cut(s) 209
CviQI GTAC 3 cut(s) 812, 1041, 1509
DdeI CTNAG 5 cut(s) 285, 483, 705, 1386, 1494
DpnI GATC 4 cut(s) 750, 801, 1275, 1362
DpnII GATC 4 cut(s) 748, 799, 1273, 1360
Eam1104I CTCTTC 2 cut(s) 348, 730
EarI CTCTTC 2 cut(s) 348, 730
Eco130I CCWWGG 2 cut(s) 372, 824
Eco147I AGGCCT 1 cut(s) 1341
Eco31I GGTCTC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 774
Eco81I CCTNAGG 1 cut(s) 1386
EcoRI GAATTC 2 cut(s) 607, 649
EcoRII CCWGG 2 cut(s) 78, 1020
EcoT14I CCWWGG 2 cut(s) 372, 824
EcoT22I ATGCAT 1 cut(s) 631
ErhI CCWWGG 2 cut(s) 372, 824
FauI CCCGC 2 cut(s) 211, 349
FauNDI CATATG 1 cut(s) 1088
Fnu4HI GCNGC 8 cut(s) 272, 318, 395, 1098, 1477, 1516, 1531, 1588
FokI GGATG 2 cut(s) 473, 1454
Fsp4HI GCNGC 8 cut(s) 272, 318, 395, 1098, 1477, 1516, 1531, 1588
FspBI CTAG 4 cut(s) 654, 921, 1512, 1551
GlaI GCGC 2 cut(s) 303, 1556
GluI GCNGC 8 cut(s) 272, 318, 395, 1098, 1477, 1516, 1531, 1588
GsaI CCCAGC 1 cut(s) 604
HaeII RGCGCY 1 cut(s) 305
HaeIII GGCC 4 cut(s) 76, 1020, 1129, 1341
HapII CCGG 1 cut(s) 210
HhaI GCGC 2 cut(s) 304, 1557
Hin6I GCGC 2 cut(s) 302, 1555
HinP1I GCGC 2 cut(s) 302, 1555
HindIII AAGCTT 4 cut(s) 777, 868, 1284, 1330
HinfI GANTC 6 cut(s) 340, 496, 550, 917, 1121, 1252
HpaII CCGG 1 cut(s) 210
HphI GGTGA 5 cut(s) 236, 923, 997, 1036, 1430
Hpy166II GTNNAC 4 cut(s) 244, 1381, 1421, 1606
Hpy188I TCNGA 5 cut(s) 555, 678, 700, 787, 1497
Hpy188III TCNNGA 8 cut(s) 493, 500, 752, 770, 997, 1256, 1291, 1430
Hpy8I GTNNAC 4 cut(s) 244, 1381, 1421, 1606
HpyAV CCTTC 2 cut(s) 813, 1331
HpyCH4III ACNGT 3 cut(s) 1045, 1137, 1262
HpyCH4IV ACGT 1 cut(s) 1596
HpyCH4V TGCA 9 cut(s) 29, 317, 397, 629, 667, 854, 1381, 1396, 1518
HpyF10VI GCNNNNNNNGC 5 cut(s) 323, 635, 1338, 1476, 1554
HpyF3I CTNAG 5 cut(s) 285, 483, 705, 1386, 1494
HpySE526I ACGT 1 cut(s) 1596
HspAI GCGC 2 cut(s) 302, 1555
Kzo9I GATC 4 cut(s) 748, 799, 1273, 1360
LmnI GCTCC 1 cut(s) 278
Lsp1109I GCAGC 7 cut(s) 283, 329, 381, 1084, 1502, 1542, 1599
LweI GCATC 2 cut(s) 967, 978
MaeI CTAG 4 cut(s) 654, 921, 1512, 1551
MaeII ACGT 1 cut(s) 1596
MaeIII GTNAC 5 cut(s) 56, 295, 582, 717, 1162
MalI GATC 4 cut(s) 750, 801, 1275, 1362
MboI GATC 4 cut(s) 748, 799, 1273, 1360
MboII GAAGA 6 cut(s) 335, 747, 767, 845, 1256, 1381
MflI RGATCY 1 cut(s) 799
MhlI GDGCHC 3 cut(s) 224, 1065, 1383
MluCI AATT 8 cut(s) 37, 51, 141, 234, 257, 607, 649, 1000
MlyI GAGTC 4 cut(s) 334, 505, 926, 1246
MmeI TCCRAC 1 cut(s) 626
Mph1103I ATGCAT 1 cut(s) 631
MseI TTAA 4 cut(s) 444, 594, 672, 761
MspA1I CMGCKG 2 cut(s) 218, 899
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 80, 1022
Mva1269I GAATGC 2 cut(s) 856, 1230
MvaI CCWGG 2 cut(s) 80, 1022
MvnI CGCG 1 cut(s) 356
MwoI GCNNNNNNNGC 5 cut(s) 323, 635, 1338, 1476, 1554
NcoI CCATGG 1 cut(s) 372
NdeI CATATG 1 cut(s) 1088
NdeII GATC 4 cut(s) 748, 799, 1273, 1360
NlaIV GGNNCC 3 cut(s) 77, 280, 1203
NmuCI GTSAC 2 cut(s) 295, 1162
NsiI ATGCAT 1 cut(s) 631
PaqCI CACCTGC 1 cut(s) 1189
PceI AGGCCT 1 cut(s) 1341
PctI GAATGC 2 cut(s) 856, 1230
PfeI GAWTC 2 cut(s) 550, 1121
PinAI ACCGGT 1 cut(s) 209
PkrI GCNGC 8 cut(s) 273, 319, 396, 1099, 1478, 1517, 1532, 1589
Ple19I CGATCG 1 cut(s) 1363
PleI GAGTC 4 cut(s) 334, 504, 925, 1246
PpsI GAGTC 4 cut(s) 334, 504, 925, 1246
Ppu21I YACGTR 1 cut(s) 1597
Psp6I CCWGG 2 cut(s) 78, 1020
PspFI CCCAGC 1 cut(s) 600
PspGI CCWGG 2 cut(s) 78, 1020
PspN4I GGNNCC 3 cut(s) 77, 280, 1203
PspPI GGNCC 2 cut(s) 75, 1127
PstI CTGCAG 1 cut(s) 1520
PsuI RGATCY 1 cut(s) 799
PvuI CGATCG 1 cut(s) 1363
PvuII CAGCTG 1 cut(s) 899
RsaI GTAC 3 cut(s) 813, 1042, 1510
RsaNI GTAC 3 cut(s) 812, 1041, 1509
SaqAI TTAA 4 cut(s) 444, 594, 672, 761
SatI GCNGC 8 cut(s) 272, 318, 395, 1098, 1477, 1516, 1531, 1588
Sau3AI GATC 4 cut(s) 748, 799, 1273, 1360
Sau96I GGNCC 2 cut(s) 75, 1127
ScaI AGTACT 1 cut(s) 1510
SchI GAGTC 4 cut(s) 334, 505, 926, 1246
ScrFI CCNGG 2 cut(s) 80, 1022
SduI GDGCHC 3 cut(s) 224, 1065, 1383
SfaNI GCATC 2 cut(s) 967, 978
SfcI CTRYAG 1 cut(s) 1516
Sse9I AATT 8 cut(s) 37, 51, 141, 234, 257, 607, 649, 1000
SseBI AGGCCT 1 cut(s) 1341
SsiI CCGC 3 cut(s) 218, 356, 1477
SspMI CTAG 4 cut(s) 654, 921, 1512, 1551
StuI AGGCCT 1 cut(s) 1341
StyD4I CCNGG 2 cut(s) 78, 1020
StyI CCWWGG 2 cut(s) 372, 824
TaaI ACNGT 3 cut(s) 1045, 1137, 1262
TaiI ACGT 1 cut(s) 1599
TaqI TCGA 3 cut(s) 492, 1290, 1359
TasI AATT 8 cut(s) 37, 51, 141, 234, 257, 607, 649, 1000
TatI WGTACW 1 cut(s) 1508
TauI GCSGC 1 cut(s) 1479
TfiI GAWTC 2 cut(s) 550, 1121
Tru1I TTAA 4 cut(s) 444, 594, 672, 761
Tru9I TTAA 4 cut(s) 444, 594, 672, 761
TscAI CASTG 5 cut(s) 229, 1276, 1486, 1525, 1564
TseFI GTSAC 2 cut(s) 295, 1162
TseI GCWGC 7 cut(s) 271, 317, 394, 1097, 1515, 1530, 1587
Tsp45I GTSAC 2 cut(s) 295, 1162
TspDTI ATGAA 5 cut(s) 17, 171, 193, 713, 894
TspGWI ACGGA 1 cut(s) 1207
TspRI CASTG 5 cut(s) 229, 1276, 1486, 1525, 1564
VneI GTGCAC 1 cut(s) 1379
XapI RAATTY 5 cut(s) 37, 141, 607, 649, 1000
XcmI CCANNNNNNNNNTGG 1 cut(s) 1580
XspI CTAG 4 cut(s) 654, 921, 1512, 1551
ZrmI AGTACT 1 cut(s) 1510
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.