Rroxscaffold_2G00096840

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017343
Physical Location & Seq
Forward (+)
18173389 .. 18180690
7302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00096840.1

Sequence Viewer

Length: 1899 bp
ATGCCTAGGTTCAAGGTGGTCAAAGGAGGGTGGATGGCAATAGGTTGTTGGGCGGTGGGTGTGGCGGCCCGTAGCATGGATGTCCCTTGCCGGGATAAAAGGAGGGTGCAAAAGCAATTAGATGAGATTCCCATTACTTCTGGGGTACTGAGAAATCTTACTTGCAAATGCATGCGGGAGTCATATAGACATCGACCGGACAGCCGGACAGGGTTAGACACACACAAACGCATCCAAAACCTTGAGAACCCAGAAAGAAGTTTAGGGGATATGGTTCTGGGAATGAAGCTGTTAGCTTTAGTATTGTCTGCACATAGAAATTTCAGCACAAAAATTGGTTACAAAAGCAAGAAATGGCCCCAGGATAGGTACAAGACCAGATACAAATCCAAAAAAGGAGAAGTTGAGAACACAAAAACCAAAATTCAATGGACTGATGAACAAAAACAAGTCATATCTGCCATTTCTAAGGGAAAATCTGTGTTCATTACCGGTTCAGCGGGCACTGGAAAAACAATTTTGGTTCAGCATATTATCAAATTGTTGAAAAAGCAGCATGGAGCCTCTAAGGTTTTTGTCACAGCGCCTACGGGTGTTGCAGCTTGTGCCATTGGGGGGCAGACTCTTCACTCTTTCGCGGGTATTGGGTATCCCATGGCTGATAGAGAGACCTTGCTGCATAGGATTGTCCTAAATAACAAGGCTTACAAAAGGTGGTTGAAAGTTAAGGCGTTGGTTATGGATGAGATTAGCATGGTGGATACTGAGTTTTTTGAGAGTCTTGAATACATTGCCAGAAAAATACGAGAAGTCAATAAAAGTTGGGGTGGGATTCAGATTGTTGTGGGTGGGGATTTCTTTCAGTTACCTCCCATTAAACCCCAGCAGAATTCAGGGGGAAAAGAATATGCATTTGAAGCCGATTGTTGGAATTCTAGCTTTGACTTGCAGGTTAATCTGACAAAGGTGTTTCGCCAATCAGACCCTCAGCTTATCAAGTTACTTCATGGAATAAGAAGAGGAGAGAGTGATCCTGAAGACTTAAAGTTTCTTGAACAAGCTTGCTCGGAGGCTGAGCCAGATCCTTCTGTGGTACAACTCTATCCAAGGATTGAAGATGTGAATAGAGTGAATGCAAGTAGATTAGAAAGCTTGGGCAATGAACTTTTTGTCTATACAGCTGTTGATAGTGGTGTGGAGTCTAGGAAACCACAGCTTGAACAGGGAATAGCCCCCAAGAAAGTTACTTTGTGTAAAGATGCGAGGGTGATGCTGGTCAAGAATTTGAATACTTGGCGTGGCCTGGTGAATGGTGCGACTGGTACTGTTCAGGGATTTTATGTGCCCAAAAATAAGGATATAACAGGCATATGCCATGCTGCTCTACCGATAGTCAAGTTTGATTCAGGGCCAGAACTGTTGATTGAACCAGAAACATGGGTTGTGACAGAGGGAGATAAGGTTGTTGCTGAACGGAAGCAGGTGCCTCTCATATTGGCATGGGCTTCAAGCATTCACAAGTGTCAGGGCATGACTCTTGACCGCCTTCACACTGATCTCTCTAAAGCTTTCGAGAATGGAATGGTTTATGTAGCTCTTTCCCGTGTGAGAAGCTTGGAAGGCCTGTATTTATCTGGTTTCGATCGCTGGAAGATTATGGTGCACCCTAAGGTTTTGCAGTTCTATAAAGGTTTTAGTGGTGAACTGGGTCAGGAGGGCAAGGATGATAATGATAGCCAGAGACAAGATAGCATGAGCCGCAGTGGTAATGGCACTCAGAGACAGGATAGTACTAGCTGCAGTGGTAATGGCAGCCAGAGACAAAGTAGCACTAGGCGCAGTGGTAATGGCAACCAGAAAGATGATGGCAGCAAATACGTGCTGGTTCACATGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PIF1

Protein Analysis

632

Amino Acids

70.76

Weight (kDa)

9.52

Isoelectric Point (pI)

39.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIF1 PF05970 145 - 329 2.1e-43 PIF1-like helicase
AAA_30 PF13604 145 - 353 1e-15 AAA domain
DEAD PF00270 145 - 271 1.6e-06 DEAD/DEAH box helicase
AAA_19 PF13245 148 - 292 3.1e-15 AAA domain
AAA_22 PF13401 158 - 281 1.5e-06 AAA domain
Pif1_2B_dom PF21530 408 - 446 9.6e-07 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017343)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g37811
prunus_persica Prupe.3G170200_v2.0.a1
pyrus_communis pycom09g07450 pycom17g13510
rosa_laevigata RLG00000020517
rosa_multiflora Rmu_sc0000185.1_g000025
rosa_roxburghii Rroxscaffold_2G00096840
rosa_rugosa Rorug02G0423300
rosa_samantha Rh2AG483700 Rh2BG496000 Rh2CG469900 Rh2DG507000
rosa_wichuraiana Rw2G039640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1471
Acc36I ACCTGC 2 cut(s) 940, 1471
AccB1I GGYRCC 1 cut(s) 1483
AccII CGCG 1 cut(s) 638
AciI CCGC 7 cut(s) 53, 65, 175, 500, 638, 1543, 1759
AclWI GGATC 2 cut(s) 1025, 1076
AcsI RAATTY 5 cut(s) 319, 423, 889, 931, 1282
AcuI CTGAAG 1 cut(s) 1056
AfaI GTAC 5 cut(s) 147, 371, 1095, 1324, 1792
AfiI CCNNNNNNNGG 5 cut(s) 76, 91, 366, 615, 927
AgeI ACCGGT 1 cut(s) 491
AjnI CCWGG 2 cut(s) 360, 1302
Alw21I GWGCWC 1 cut(s) 1665
Alw26I GTCTC 4 cut(s) 662, 1735, 1774, 1813
Alw44I GTGCAC 1 cut(s) 1661
AlwI GGATC 2 cut(s) 1025, 1076
AoxI GGCC 5 cut(s) 66, 356, 1300, 1409, 1621
ApaLI GTGCAC 1 cut(s) 1661
ApeKI GCWGC 7 cut(s) 553, 599, 676, 1379, 1797, 1812, 1869
ApoI RAATTY 5 cut(s) 319, 423, 889, 931, 1282
AsiGI ACCGGT 1 cut(s) 491
AspA2I CCTAGG 1 cut(s) 5
AspLEI GCGC 2 cut(s) 586, 1839
AspS9I GGNCC 3 cut(s) 67, 357, 1409
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 3 cut(s) 1279, 1318, 1712
AvrII CCTAGG 1 cut(s) 5
AxyI CCTNAGG 1 cut(s) 1668
BaeGI GKGCMC 3 cut(s) 506, 1347, 1665
BanI GGYRCC 1 cut(s) 1483
BbsI GAAGAC 1 cut(s) 1044
Bbv12I GWGCWC 1 cut(s) 1665
BbvCI CCTCAGC 1 cut(s) 987
BbvI GCAGC 7 cut(s) 565, 611, 663, 1366, 1784, 1824, 1881
BccI CCATC 2 cut(s) 28, 1859
BcgI CGANNNNNNTGC 2 cut(s) 1252, 1286
BciT130I CCWGG 2 cut(s) 362, 1304
BciVI GTATCC 2 cut(s) 660, 754
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 4 cut(s) 662, 1735, 1774, 1813
BfaI CTAG 5 cut(s) 6, 936, 1203, 1794, 1833
BfmI CTRYAG 1 cut(s) 1798
BfoI RGCGCY 1 cut(s) 587
BfuAI ACCTGC 2 cut(s) 940, 1471
BfuI GTATCC 2 cut(s) 660, 754
BisI GCNGC 9 cut(s) 66, 554, 600, 677, 1380, 1759, 1798, 1813, 1870
BlnI CCTAGG 1 cut(s) 5
BlpI GCTNAGC 1 cut(s) 1074
BlsI GCNGC 9 cut(s) 67, 555, 601, 678, 1381, 1760, 1799, 1814, 1871
BmcAI AGTACT 1 cut(s) 1792
Bme1390I CCNGG 3 cut(s) 92, 362, 1304
BmgT120I GGNCC 3 cut(s) 67, 357, 1409
BmiI GGNNCC 3 cut(s) 359, 562, 1485
BmrFI CCNGG 3 cut(s) 92, 362, 1304
BmrI ACTGGG 1 cut(s) 1715
BmsI GCATC 3 cut(s) 240, 1249, 1260
BmuI ACTGGG 1 cut(s) 1715
BpiI GAAGAC 1 cut(s) 1044
Bpu10I CCTNAGC 1 cut(s) 987
Bpu1102I GCTNAGC 1 cut(s) 1074
BpuEI CTTGAG 1 cut(s) 263
BpuMI CCSGG 1 cut(s) 92
BsaAI YACGTR 1 cut(s) 1879
BsaBI GATNNNNATC 1 cut(s) 385
BsaI GGTCTC 1 cut(s) 662
BsaJI CCNNGG 4 cut(s) 5, 360, 654, 1106
BsaWI WCCGGW 2 cut(s) 196, 491
BsaXI ACNNNNNCTCC 2 cut(s) 1446, 1476
Bsc4I CCNNNNNNNGG 5 cut(s) 76, 91, 366, 615, 927
Bse118I RCCGGY 1 cut(s) 491
Bse1I ACTGG 3 cut(s) 511, 1324, 1710
Bse21I CCTNAGG 1 cut(s) 1668
Bse3DI GCAATG 2 cut(s) 789, 1165
Bse8I GATNNNNATC 1 cut(s) 385
BseBI CCWGG 2 cut(s) 362, 1304
BseDI CCNNGG 4 cut(s) 5, 360, 654, 1106
BseGI GGATG 5 cut(s) 39, 85, 231, 748, 1729
BseJI GATNNNNATC 1 cut(s) 385
BseLI CCNNNNNNNGG 5 cut(s) 76, 91, 366, 615, 927
BseMI GCAATG 2 cut(s) 789, 1165
BseMII CTCAG 5 cut(s) 140, 756, 1001, 1065, 1790
BseNI ACTGG 3 cut(s) 511, 1324, 1710
BseRI GAGGAG 1 cut(s) 1035
BseSI GKGCMC 3 cut(s) 506, 1347, 1665
BseXI GCAGC 7 cut(s) 565, 611, 663, 1366, 1784, 1824, 1881
BseYI CCCAGC 1 cut(s) 882
BsgI GTGCAG 1 cut(s) 294
Bsh1236I CGCG 1 cut(s) 638
Bsh1285I CGRYCG 2 cut(s) 197, 1645
BshFI GGCC 5 cut(s) 68, 358, 1302, 1411, 1623
BshNI GGYRCC 1 cut(s) 1483
BshTI ACCGGT 1 cut(s) 491
BsiEI CGRYCG 2 cut(s) 197, 1645
BsiHKAI GWGCWC 1 cut(s) 1665
BsiSI CCGG 4 cut(s) 91, 197, 205, 492
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 5 cut(s) 76, 91, 366, 615, 927
BsmAI GTCTC 4 cut(s) 662, 1735, 1774, 1813
BsmFI GGGAC 1 cut(s) 68
BsmI GAATGC 2 cut(s) 1138, 1512
BsnI GGCC 5 cut(s) 68, 358, 1302, 1411, 1623
Bso31I GGTCTC 1 cut(s) 662
Bsp1286I GDGCHC 3 cut(s) 506, 1347, 1665
Bsp143I GATC 4 cut(s) 1030, 1081, 1555, 1642
Bsp1720I GCTNAGC 1 cut(s) 1074
Bsp19I CCATGG 1 cut(s) 654
BspACI CCGC 7 cut(s) 53, 65, 175, 500, 638, 1543, 1759
BspANI GGCC 5 cut(s) 68, 358, 1302, 1411, 1623
BspCNI CTCAG 5 cut(s) 141, 757, 1000, 1066, 1789
BspFNI CGCG 1 cut(s) 638
BspLI GGNNCC 3 cut(s) 359, 562, 1485
BspMAI CTGCAG 1 cut(s) 1802
BspMI ACCTGC 2 cut(s) 940, 1471
BspPI GGATC 2 cut(s) 1025, 1076
BspT107I GGYRCC 1 cut(s) 1483
BspTNI GGTCTC 1 cut(s) 662
BsrDI GCAATG 2 cut(s) 789, 1165
BsrFI RCCGGY 1 cut(s) 491
BsrI ACTGG 3 cut(s) 511, 1324, 1710
BssAI RCCGGY 1 cut(s) 491
BssECI CCNNGG 4 cut(s) 5, 360, 654, 1106
BssMI GATC 4 cut(s) 1030, 1081, 1555, 1642
BssT1I CCWWGG 3 cut(s) 5, 654, 1106
Bst2UI CCWGG 2 cut(s) 362, 1304
Bst4CI ACNGT 2 cut(s) 1327, 1419
Bst6I CTCTTC 2 cut(s) 630, 1012
BstAPI GCANNNNNTGC 1 cut(s) 605
BstBAI YACGTR 1 cut(s) 1879
BstC8I GCNNGC 3 cut(s) 173, 502, 1063
BstDEI CTNAG 8 cut(s) 149, 468, 567, 765, 987, 1074, 1668, 1776
BstDSI CCRYGG 1 cut(s) 654
BstF5I GGATG 5 cut(s) 39, 85, 231, 748, 1729
BstFNI CGCG 1 cut(s) 638
BstH2I RGCGCY 1 cut(s) 587
BstHHI GCGC 2 cut(s) 586, 1839
BstKTI GATC 4 cut(s) 1033, 1084, 1558, 1645
BstMAI GTCTC 4 cut(s) 662, 1735, 1774, 1813
BstMBI GATC 4 cut(s) 1030, 1081, 1555, 1642
BstMCI CGRYCG 2 cut(s) 197, 1645
BstMWI GCNNNNNNNGC 5 cut(s) 605, 917, 1620, 1758, 1836
BstNI CCWGG 2 cut(s) 362, 1304
BstNSI RCATGY 1 cut(s) 175
BstSCI CCNGG 3 cut(s) 90, 360, 1302
BstSFI CTRYAG 1 cut(s) 1798
BstSLI GKGCMC 3 cut(s) 506, 1347, 1665
BstUI CGCG 1 cut(s) 638
BstV1I GCAGC 7 cut(s) 565, 611, 663, 1366, 1784, 1824, 1881
BstV2I GAAGAC 1 cut(s) 1044
BstX2I RGATCY 1 cut(s) 1081
BstXI CCANNNNNNTGG 1 cut(s) 1437
BstYI RGATCY 1 cut(s) 1081
Bsu36I CCTNAGG 1 cut(s) 1668
BsuI GTATCC 2 cut(s) 660, 754
BsuRI GGCC 5 cut(s) 68, 358, 1302, 1411, 1623
BtgI CCRYGG 1 cut(s) 654
BtsCI GGATG 5 cut(s) 39, 85, 231, 748, 1729
BtsI GCAGTG 3 cut(s) 1768, 1807, 1846
BtsIMutI CAGTG 5 cut(s) 504, 1551, 1768, 1807, 1846
BveI ACCTGC 2 cut(s) 940, 1471
Cac8I GCNNGC 3 cut(s) 173, 502, 1063
CfoI GCGC 2 cut(s) 586, 1839
Cfr10I RCCGGY 1 cut(s) 491
Cfr13I GGNCC 3 cut(s) 67, 357, 1409
Csp6I GTAC 5 cut(s) 146, 370, 1094, 1323, 1791
CspAI ACCGGT 1 cut(s) 491
CviQI GTAC 5 cut(s) 146, 370, 1094, 1323, 1791
DdeI CTNAG 8 cut(s) 149, 468, 567, 765, 987, 1074, 1668, 1776
DpnI GATC 4 cut(s) 1032, 1083, 1557, 1644
DpnII GATC 4 cut(s) 1030, 1081, 1555, 1642
Eam1104I CTCTTC 2 cut(s) 630, 1012
EarI CTCTTC 2 cut(s) 630, 1012
Eco130I CCWWGG 3 cut(s) 5, 654, 1106
Eco147I AGGCCT 1 cut(s) 1623
Eco31I GGTCTC 1 cut(s) 662
Eco57I CTGAAG 1 cut(s) 1056
Eco81I CCTNAGG 1 cut(s) 1668
EcoRI GAATTC 2 cut(s) 889, 931
EcoRII CCWGG 2 cut(s) 360, 1302
EcoT14I CCWWGG 3 cut(s) 5, 654, 1106
EcoT22I ATGCAT 2 cut(s) 173, 913
ErhI CCWWGG 3 cut(s) 5, 654, 1106
FaqI GGGAC 1 cut(s) 68
FauI CCCGC 3 cut(s) 168, 493, 631
FauNDI CATATG 1 cut(s) 1370
Fnu4HI GCNGC 9 cut(s) 66, 554, 600, 677, 1380, 1759, 1798, 1813, 1870
FokI GGATG 5 cut(s) 46, 92, 218, 755, 1736
Fsp4HI GCNGC 9 cut(s) 66, 554, 600, 677, 1380, 1759, 1798, 1813, 1870
FspBI CTAG 5 cut(s) 6, 936, 1203, 1794, 1833
GlaI GCGC 2 cut(s) 585, 1838
GluI GCNGC 9 cut(s) 66, 554, 600, 677, 1380, 1759, 1798, 1813, 1870
GsaI CCCAGC 1 cut(s) 886
HaeII RGCGCY 1 cut(s) 587
HaeIII GGCC 5 cut(s) 68, 358, 1302, 1411, 1623
HapII CCGG 4 cut(s) 91, 197, 205, 492
HhaI GCGC 2 cut(s) 586, 1839
Hin6I GCGC 2 cut(s) 584, 1837
HinP1I GCGC 2 cut(s) 584, 1837
HindIII AAGCTT 4 cut(s) 1059, 1150, 1566, 1612
HinfI GANTC 8 cut(s) 127, 179, 622, 778, 832, 1199, 1403, 1534
HpaII CCGG 4 cut(s) 91, 197, 205, 492
HphI GGTGA 3 cut(s) 1279, 1318, 1712
Hpy166II GTNNAC 3 cut(s) 1663, 1703, 1888
Hpy188I TCNGA 5 cut(s) 837, 960, 982, 1069, 1779
Hpy188III TCNNGA 7 cut(s) 782, 1034, 1052, 1279, 1538, 1573, 1712
Hpy8I GTNNAC 3 cut(s) 1663, 1703, 1888
HpyAV CCTTC 3 cut(s) 1095, 1556, 1613
HpyCH4III ACNGT 2 cut(s) 1327, 1419
HpyCH4IV ACGT 1 cut(s) 1878
HpyF10VI GCNNNNNNNGC 5 cut(s) 605, 917, 1620, 1758, 1836
HpyF3I CTNAG 8 cut(s) 149, 468, 567, 765, 987, 1074, 1668, 1776
HpySE526I ACGT 1 cut(s) 1878
HspAI GCGC 2 cut(s) 584, 1837
Kzo9I GATC 4 cut(s) 1030, 1081, 1555, 1642
LmnI GCTCC 1 cut(s) 560
Lsp1109I GCAGC 7 cut(s) 565, 611, 663, 1366, 1784, 1824, 1881
LweI GCATC 3 cut(s) 240, 1249, 1260
MaeI CTAG 5 cut(s) 6, 936, 1203, 1794, 1833
MaeII ACGT 1 cut(s) 1878
MaeIII GTNAC 6 cut(s) 338, 577, 864, 999, 1243, 1444
MalI GATC 4 cut(s) 1032, 1083, 1557, 1644
MboI GATC 4 cut(s) 1030, 1081, 1555, 1642
MboII GAAGA 5 cut(s) 617, 1029, 1049, 1127, 1663
MflI RGATCY 1 cut(s) 1081
MhlI GDGCHC 3 cut(s) 506, 1347, 1665
MluCI AATT 9 cut(s) 116, 319, 333, 423, 516, 539, 889, 931, 1282
MlyI GAGTC 5 cut(s) 188, 616, 787, 1208, 1528
MmeI TCCRAC 1 cut(s) 908
Mph1103I ATGCAT 2 cut(s) 173, 913
MseI TTAA 4 cut(s) 726, 876, 954, 1043
MspA1I CMGCKG 2 cut(s) 500, 1181
MspI CCGG 4 cut(s) 91, 197, 205, 492
MspR9I CCNGG 3 cut(s) 92, 362, 1304
Mva1269I GAATGC 2 cut(s) 1138, 1512
MvaI CCWGG 2 cut(s) 362, 1304
MvnI CGCG 1 cut(s) 638
MwoI GCNNNNNNNGC 5 cut(s) 605, 917, 1620, 1758, 1836
NciI CCSGG 1 cut(s) 92
NcoI CCATGG 1 cut(s) 654
NdeI CATATG 1 cut(s) 1370
NdeII GATC 4 cut(s) 1030, 1081, 1555, 1642
NlaIV GGNNCC 3 cut(s) 359, 562, 1485
NmuCI GTSAC 2 cut(s) 577, 1444
NsiI ATGCAT 2 cut(s) 173, 913
NspI RCATGY 1 cut(s) 175
PaeI GCATGC 1 cut(s) 175
PaqCI CACCTGC 1 cut(s) 1471
PceI AGGCCT 1 cut(s) 1623
PctI GAATGC 2 cut(s) 1138, 1512
PfeI GAWTC 3 cut(s) 127, 832, 1403
PinAI ACCGGT 1 cut(s) 491
PkrI GCNGC 9 cut(s) 67, 555, 601, 678, 1381, 1760, 1799, 1814, 1871
Ple19I CGATCG 1 cut(s) 1645
PleI GAGTC 5 cut(s) 187, 616, 786, 1207, 1528
PpsI GAGTC 5 cut(s) 187, 616, 786, 1207, 1528
Ppu21I YACGTR 1 cut(s) 1879
Psp6I CCWGG 2 cut(s) 360, 1302
PspFI CCCAGC 1 cut(s) 882
PspGI CCWGG 2 cut(s) 360, 1302
PspN4I GGNNCC 3 cut(s) 359, 562, 1485
PspPI GGNCC 3 cut(s) 67, 357, 1409
PstI CTGCAG 1 cut(s) 1802
PsuI RGATCY 1 cut(s) 1081
PvuI CGATCG 1 cut(s) 1645
PvuII CAGCTG 1 cut(s) 1181
RsaI GTAC 5 cut(s) 147, 371, 1095, 1324, 1792
RsaNI GTAC 5 cut(s) 146, 370, 1094, 1323, 1791
SaqAI TTAA 4 cut(s) 726, 876, 954, 1043
SatI GCNGC 9 cut(s) 66, 554, 600, 677, 1380, 1759, 1798, 1813, 1870
Sau3AI GATC 4 cut(s) 1030, 1081, 1555, 1642
Sau96I GGNCC 3 cut(s) 67, 357, 1409
ScaI AGTACT 1 cut(s) 1792
SchI GAGTC 5 cut(s) 188, 616, 787, 1208, 1528
ScrFI CCNGG 3 cut(s) 92, 362, 1304
SduI GDGCHC 3 cut(s) 506, 1347, 1665
SfaNI GCATC 3 cut(s) 240, 1249, 1260
SfcI CTRYAG 1 cut(s) 1798
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
SphI GCATGC 1 cut(s) 175
Sse9I AATT 9 cut(s) 116, 319, 333, 423, 516, 539, 889, 931, 1282
SseBI AGGCCT 1 cut(s) 1623
SsiI CCGC 7 cut(s) 53, 65, 175, 500, 638, 1543, 1759
SspMI CTAG 5 cut(s) 6, 936, 1203, 1794, 1833
StuI AGGCCT 1 cut(s) 1623
StyD4I CCNGG 3 cut(s) 90, 360, 1302
StyI CCWWGG 3 cut(s) 5, 654, 1106
TaaI ACNGT 2 cut(s) 1327, 1419
TaiI ACGT 1 cut(s) 1881
TaqI TCGA 3 cut(s) 193, 1572, 1641
TasI AATT 9 cut(s) 116, 319, 333, 423, 516, 539, 889, 931, 1282
TatI WGTACW 1 cut(s) 1790
TauI GCSGC 2 cut(s) 68, 1761
TfiI GAWTC 3 cut(s) 127, 832, 1403
Tru1I TTAA 4 cut(s) 726, 876, 954, 1043
Tru9I TTAA 4 cut(s) 726, 876, 954, 1043
TscAI CASTG 5 cut(s) 511, 1558, 1768, 1807, 1846
TseFI GTSAC 2 cut(s) 577, 1444
TseI GCWGC 7 cut(s) 553, 599, 676, 1379, 1797, 1812, 1869
Tsp45I GTSAC 2 cut(s) 577, 1444
TspDTI ATGAA 5 cut(s) 299, 453, 475, 995, 1176
TspGWI ACGGA 1 cut(s) 1489
TspRI CASTG 5 cut(s) 511, 1558, 1768, 1807, 1846
VneI GTGCAC 1 cut(s) 1661
XapI RAATTY 5 cut(s) 319, 423, 889, 931, 1282
XceI RCATGY 1 cut(s) 175
XcmI CCANNNNNNNNNTGG 1 cut(s) 1862
XmaJI CCTAGG 1 cut(s) 5
XspI CTAG 5 cut(s) 6, 936, 1203, 1794, 1833
ZrmI AGTACT 1 cut(s) 1792
Zsp2I ATGCAT 2 cut(s) 173, 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.