pycom09g07450

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0017343
Physical Location & Seq
Forward (+)
5647745 .. 5649323
1579 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g07450.2

Sequence Viewer

Length: 909 bp
ATGCTATGTGTGAAGTTTATTGCCATGCATTTTCTACGCAGAGAAATTTTAGTACCAAAACTAGTGGAAAAAGCATTAATTGGGTTCAGGATACATACAACCTCAGATACAAACCCGAAACTCCTAGAGTTAAGTGGACTGACCACCAGAAACAAGTCATATCTGCCATTTCTGAGGGGAAATCTGTGTCCATTACCGGTTCCGCGGGGACCAGAGAAACGATGTTGGTTAAGCACATCATCATCGACAAGATGTCATACACCTTCGAAGGTTTTTGTCACTGCACCTACCGGTGCTGCGGCTTGTGCTATCCGTGGGCAGACCCTTCACTCTTTCGCTGGTATTGGATGTGGCAATGCTGATAGTATAACTTTGCTGAGTAGGGTTCTCGTGAACAAGGAGGCTTGTAGGAGGTGGTTGAAAGCCGAAGTGTTGGTCATAGATGAGGTTAGCATGGTTGACGCGGAGCGTGGCCAGGTGAATGGTGCAACTGGTACTGCTATGGCATTTCTTAAGACCAAAGATGTGGGAGTGATGAGCATATGCGATGCTGGGCTGCTACCAGTAGTTAAATTTGATTTGGGGATGACAAAGGTGATTAAAACAAAAACATGGGATGTGACAGAAGGAGATCCAGTAGTTGCTCAAAGAGAGCAGCAACTTTCACACTGGCTTGGGCTTCAAGCATTCACAAGTGCCAGGGAGGTTGAGGATAATGTCAGCCAGAAAGAATATCGTAGCAATGATAATAGTGGCCGGGAAACAAAGATGAGGAGGTTGAGAATGAGGATGCCATCTAGAACAAATATCGTAGGAAGGATACCGATAGAAAAGGAAATGAGGGCAGCGGTTATACCATACACTTGGAACACCTGTAGGAAGAAAATACCAGGAAAACAACTGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PIF1

Protein Analysis

303

Amino Acids

33.68

Weight (kDa)

9.91

Isoelectric Point (pI)

44.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017343)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g37811
prunus_persica Prupe.3G170200_v2.0.a1
pyrus_communis pycom09g07450 pycom17g13510
rosa_laevigata RLG00000020517
rosa_multiflora Rmu_sc0000185.1_g000025
rosa_roxburghii Rroxscaffold_2G00096840
rosa_rugosa Rorug02G0423300
rosa_samantha Rh2AG483700 Rh2BG496000 Rh2CG469900 Rh2DG507000
rosa_wichuraiana Rw2G039640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 205, 464
AciI CCGC 5 cut(s) 203, 205, 299, 464, 848
AclWI GGATC 1 cut(s) 626
AcoI YGGCCR 2 cut(s) 472, 754
AcsI RAATTY 2 cut(s) 45, 572
AfaI GTAC 2 cut(s) 54, 496
AflII CTTAAG 1 cut(s) 512
AgeI ACCGGT 2 cut(s) 196, 290
AgsI TTSAA 2 cut(s) 421, 683
AhdI GACNNNNNGTC 1 cut(s) 252
AhlI ACTAGT 1 cut(s) 61
AjnI CCWGG 3 cut(s) 474, 698, 889
AlwI GGATC 1 cut(s) 626
AoxI GGCC 2 cut(s) 472, 754
ApeKI GCWGC 4 cut(s) 296, 556, 655, 845
ApoI RAATTY 2 cut(s) 45, 572
ArsI GACNNNNNNTTYG 2 cut(s) 420, 452
AseI ATTAAT 1 cut(s) 77
AsiGI ACCGGT 2 cut(s) 196, 290
AspS9I GGNCC 1 cut(s) 209
AsuC2I CCSGG 1 cut(s) 758
AsuHPI GGTGA 2 cut(s) 490, 607
AsuII TTCGAA 1 cut(s) 266
AvaII GGWCC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 474
BauI CACGAG 1 cut(s) 389
BbvI GCAGC 4 cut(s) 283, 543, 667, 857
BccI CCATC 1 cut(s) 802
BciT130I CCWGG 3 cut(s) 476, 700, 891
BciVI GTATCC 2 cut(s) 84, 813
BcnI CCSGG 1 cut(s) 758
BcuI ACTAGT 1 cut(s) 61
BfaI CTAG 3 cut(s) 62, 125, 798
BfmI CTRYAG 1 cut(s) 874
BfrI CTTAAG 1 cut(s) 512
BfuI GTATCC 2 cut(s) 84, 813
BisI GCNGC 5 cut(s) 297, 300, 557, 656, 846
BlsI GCNGC 5 cut(s) 298, 301, 558, 657, 847
Bme1390I CCNGG 4 cut(s) 476, 700, 758, 891
Bme18I GGWCC 1 cut(s) 209
BmeRI GACNNNNNGTC 1 cut(s) 252
BmgT120I GGNCC 1 cut(s) 209
BmiI GGNNCC 2 cut(s) 201, 210
BmrFI CCNGG 4 cut(s) 476, 700, 758, 891
BmsI GCATC 2 cut(s) 538, 780
Bpu14I TTCGAA 1 cut(s) 266
BpuMI CCSGG 1 cut(s) 758
BsaJI CCNNGG 3 cut(s) 203, 313, 699
BsaWI WCCGGW 2 cut(s) 196, 290
BsaXI ACNNNNNCTCC 2 cut(s) 621, 651
Bse118I RCCGGY 2 cut(s) 196, 290
Bse1I ACTGG 4 cut(s) 496, 563, 635, 674
Bse3DI GCAATG 2 cut(s) 361, 748
BseBI CCWGG 3 cut(s) 476, 700, 891
BseDI CCNNGG 3 cut(s) 203, 313, 699
BseGI GGATG 4 cut(s) 353, 591, 622, 795
BseMI GCAATG 2 cut(s) 361, 748
BseMII CTCAG 4 cut(s) 117, 164, 368, 893
BseNI ACTGG 4 cut(s) 496, 563, 635, 674
BseRI GAGGAG 1 cut(s) 787
BseXI GCAGC 4 cut(s) 283, 543, 667, 857
BseYI CCCAGC 1 cut(s) 551
BsgI GTGCAG 1 cut(s) 267
Bsh1236I CGCG 2 cut(s) 205, 464
BshFI GGCC 2 cut(s) 474, 756
BshTI ACCGGT 2 cut(s) 196, 290
BsiSI CCGG 3 cut(s) 197, 291, 757
BslFI GGGAC 1 cut(s) 222
BsmFI GGGAC 1 cut(s) 222
BsmI GAATGC 1 cut(s) 686
BsnI GGCC 2 cut(s) 474, 756
Bsp119I TTCGAA 1 cut(s) 266
Bsp143I GATC 1 cut(s) 631
BspACI CCGC 5 cut(s) 203, 205, 299, 464, 848
BspANI GGCC 2 cut(s) 474, 756
BspCNI CTCAG 4 cut(s) 116, 165, 369, 894
BspFNI CGCG 2 cut(s) 205, 464
BspLI GGNNCC 2 cut(s) 201, 210
BspPI GGATC 1 cut(s) 626
BspT104I TTCGAA 1 cut(s) 266
BspTI CTTAAG 1 cut(s) 512
BsrDI GCAATG 2 cut(s) 361, 748
BsrFI RCCGGY 2 cut(s) 196, 290
BsrI ACTGG 4 cut(s) 496, 563, 635, 674
BssAI RCCGGY 2 cut(s) 196, 290
BssECI CCNNGG 3 cut(s) 203, 313, 699
BssMI GATC 1 cut(s) 631
BssSI CACGAG 1 cut(s) 389
Bst2BI CACGAG 1 cut(s) 389
Bst2UI CCWGG 3 cut(s) 476, 700, 891
BstAFI CTTAAG 1 cut(s) 512
BstBI TTCGAA 1 cut(s) 266
BstDEI CTNAG 4 cut(s) 103, 173, 377, 902
BstDSI CCRYGG 2 cut(s) 203, 313
BstF5I GGATG 4 cut(s) 353, 591, 622, 795
BstFNI CGCG 2 cut(s) 205, 464
BstKTI GATC 1 cut(s) 634
BstMBI GATC 1 cut(s) 631
BstMWI GCNNNNNNNGC 1 cut(s) 305
BstNI CCWGG 3 cut(s) 476, 700, 891
BstSCI CCNGG 4 cut(s) 474, 698, 756, 889
BstSFI CTRYAG 1 cut(s) 874
BstUI CGCG 2 cut(s) 205, 464
BstV1I GCAGC 4 cut(s) 283, 543, 667, 857
BstX2I RGATCY 1 cut(s) 631
BstXI CCANNNNNNTGG 3 cut(s) 482, 526, 864
BstYI RGATCY 1 cut(s) 631
BsuI GTATCC 2 cut(s) 84, 813
BsuRI GGCC 2 cut(s) 474, 756
BtgI CCRYGG 2 cut(s) 203, 313
BtgZI GCGATG 1 cut(s) 561
BtsCI GGATG 4 cut(s) 353, 591, 622, 795
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 279, 667
Cfr10I RCCGGY 2 cut(s) 196, 290
Cfr13I GGNCC 1 cut(s) 209
Cfr42I CCGCGG 1 cut(s) 206
CseI GACGC 1 cut(s) 470
Csp6I GTAC 2 cut(s) 53, 495
CspAI ACCGGT 2 cut(s) 196, 290
CspCI CAANNNNNGTGG 2 cut(s) 45, 80
CviAII CATG 3 cut(s) 25, 454, 612
CviJI RGCY 9 cut(s) 302, 404, 425, 474, 556, 673, 679, 723, 756
CviKI_1 RGCY 9 cut(s) 302, 404, 425, 474, 556, 673, 679, 723, 756
CviQI GTAC 2 cut(s) 53, 495
DdeI CTNAG 4 cut(s) 103, 173, 377, 902
DpnI GATC 1 cut(s) 633
DpnII GATC 1 cut(s) 631
DriI GACNNNNNGTC 1 cut(s) 252
EaeI YGGCCR 2 cut(s) 472, 754
Eam1105I GACNNNNNGTC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 209
EcoRII CCWGG 3 cut(s) 474, 698, 889
EcoT22I ATGCAT 1 cut(s) 30
FaeI CATG 3 cut(s) 28, 457, 615
FaqI GGGAC 1 cut(s) 222
FatI CATG 3 cut(s) 24, 453, 611
FauI CCCGC 1 cut(s) 198
FauNDI CATATG 1 cut(s) 542
Fnu4HI GCNGC 5 cut(s) 297, 300, 557, 656, 846
FokI GGATG 4 cut(s) 360, 598, 629, 802
Fsp4HI GCNGC 5 cut(s) 297, 300, 557, 656, 846
FspBI CTAG 3 cut(s) 62, 125, 798
GluI GCNGC 5 cut(s) 297, 300, 557, 656, 846
GsaI CCCAGC 1 cut(s) 555
HaeIII GGCC 2 cut(s) 474, 756
HapII CCGG 3 cut(s) 197, 291, 757
HgaI GACGC 1 cut(s) 470
Hin1II CATG 3 cut(s) 28, 457, 615
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HpaII CCGG 3 cut(s) 197, 291, 757
HphI GGTGA 2 cut(s) 490, 607
Hpy166II GTNNAC 3 cut(s) 137, 394, 460
Hpy188I TCNGA 2 cut(s) 106, 174
Hpy188III TCNNGA 3 cut(s) 88, 391, 798
Hpy8I GTNNAC 3 cut(s) 137, 394, 460
HpyAV CCTTC 5 cut(s) 262, 273, 335, 620, 810
HpyCH4V TGCA 3 cut(s) 28, 284, 488
HpyF10VI GCNNNNNNNGC 1 cut(s) 305
HpyF3I CTNAG 4 cut(s) 103, 173, 377, 902
Hsp92II CATG 3 cut(s) 28, 457, 615
KspI CCGCGG 1 cut(s) 206
Kzo9I GATC 1 cut(s) 631
LmnI GCTCC 1 cut(s) 466
Lsp1109I GCAGC 4 cut(s) 283, 543, 667, 857
LweI GCATC 2 cut(s) 538, 780
MaeI CTAG 3 cut(s) 62, 125, 798
MaeIII GTNAC 2 cut(s) 277, 619
MalI GATC 1 cut(s) 633
MboI GATC 1 cut(s) 631
MboII GAAGA 1 cut(s) 892
MflI RGATCY 1 cut(s) 631
MlsI TGGCCA 1 cut(s) 474
MluCI AATT 3 cut(s) 45, 78, 572
MluNI TGGCCA 1 cut(s) 474
Mox20I TGGCCA 1 cut(s) 474
Mph1103I ATGCAT 1 cut(s) 30
MscI TGGCCA 1 cut(s) 474
MseI TTAA 6 cut(s) 77, 131, 230, 513, 570, 600
Msp20I TGGCCA 1 cut(s) 474
MspA1I CMGCKG 2 cut(s) 205, 848
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 3 cut(s) 197, 291, 757
MspR9I CCNGG 4 cut(s) 476, 700, 758, 891
Mva1269I GAATGC 1 cut(s) 686
MvaI CCWGG 3 cut(s) 476, 700, 891
MvnI CGCG 2 cut(s) 205, 464
MwoI GCNNNNNNNGC 1 cut(s) 305
NciI CCSGG 1 cut(s) 758
NdeI CATATG 1 cut(s) 542
NdeII GATC 1 cut(s) 631
NlaIII CATG 3 cut(s) 28, 457, 615
NlaIV GGNNCC 2 cut(s) 201, 210
NmuCI GTSAC 2 cut(s) 277, 619
NsiI ATGCAT 1 cut(s) 30
NspV TTCGAA 1 cut(s) 266
PctI GAATGC 1 cut(s) 686
PinAI ACCGGT 2 cut(s) 196, 290
PkrI GCNGC 5 cut(s) 298, 301, 558, 657, 847
PshBI ATTAAT 1 cut(s) 77
Psp6I CCWGG 3 cut(s) 474, 698, 889
PspFI CCCAGC 1 cut(s) 551
PspGI CCWGG 3 cut(s) 474, 698, 889
PspN4I GGNNCC 2 cut(s) 201, 210
PspPI GGNCC 1 cut(s) 209
PsuI RGATCY 1 cut(s) 631
RsaI GTAC 2 cut(s) 54, 496
RsaNI GTAC 2 cut(s) 53, 495
SacII CCGCGG 1 cut(s) 206
SaqAI TTAA 6 cut(s) 77, 131, 230, 513, 570, 600
SatI GCNGC 5 cut(s) 297, 300, 557, 656, 846
Sau3AI GATC 1 cut(s) 631
Sau96I GGNCC 1 cut(s) 209
ScrFI CCNGG 4 cut(s) 476, 700, 758, 891
SfaNI GCATC 2 cut(s) 538, 780
SfcI CTRYAG 1 cut(s) 874
Sfr303I CCGCGG 1 cut(s) 206
SfuI TTCGAA 1 cut(s) 266
SgrBI CCGCGG 1 cut(s) 206
SinI GGWCC 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 512
SmoI CTYRAG 1 cut(s) 512
SpeI ACTAGT 1 cut(s) 61
Sse9I AATT 3 cut(s) 45, 78, 572
SsiI CCGC 5 cut(s) 203, 205, 299, 464, 848
SspMI CTAG 3 cut(s) 62, 125, 798
StyD4I CCNGG 4 cut(s) 474, 698, 756, 889
TaqI TCGA 2 cut(s) 245, 266
TasI AATT 3 cut(s) 45, 78, 572
TauI GCSGC 1 cut(s) 302
Tru1I TTAA 6 cut(s) 77, 131, 230, 513, 570, 600
Tru9I TTAA 6 cut(s) 77, 131, 230, 513, 570, 600
TscAI CASTG 2 cut(s) 286, 674
TseFI GTSAC 2 cut(s) 277, 619
TseI GCWGC 4 cut(s) 296, 556, 655, 845
Tsp45I GTSAC 2 cut(s) 277, 619
TspGWI ACGGA 1 cut(s) 302
TspRI CASTG 2 cut(s) 286, 674
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 1 cut(s) 209
VspI ATTAAT 1 cut(s) 77
XapI RAATTY 2 cut(s) 45, 572
XbaI TCTAGA 1 cut(s) 797
XspI CTAG 3 cut(s) 62, 125, 798
Zsp2I ATGCAT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.