RLG00000020517

ATP-dependent DNA helicase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017343
Physical Location & Seq
Reverse (-)
65871476 .. 65873104
1629 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020517

Sequence Viewer

Length: 1629 bp
ATGGTTCTGGGAATGAAGCTGTTAGCTTTAGTATTGTCTGCACACAGAAATTTCAGCACAAAAATTGGTTACAAAAGCAAGAAATGGCCCCAGGATAGATACAAGGCCAGATACAAACCCAAAAAGGGAGAAGTTGGGAACACAAAAACCAAAATTCGATGGACTGATGAACAAAAACAAGTCATATCTGCCATTTCTAAAGGAAAATCTGTGTTCATTACCGGTTCAGCGGGCACTGGAAAAACAATTTTGGTTCAGCATATTATCAGAATGTTGAAAAAGCAGCATGGAGCCTCTAAGGTTTTTGTCACAGCGCCTACGGGTGTTGCGGCTTGTGCCATTGGGGGGCAGACTCTTCACTCTTTTGCGGGTATTGGGTATCCCATGGCTGATAGAGAGACCTTGCTGCATAGGATTGTCCTAAATAACAAGGCTTACAACAGGTGGTTGAAGGTTAAGGCATTGGTTATGGATGAGATTAGCATGGTGGATACTGAGTTTTTCGAGAGTCTTGAATACATTGCCAGAAAAATACGAGAAGTCAATAAAAGTTGGGGTGGGATTCAGATTGTTGTGGGTGGGGATTTCTTTCAGTTACCTCCCATTAAACCCCAGCAGAATTCAGGGGGAAAAGAATATGCATTTGAAGCCGATTGTTGGAATTCTAGCTTTGACTTGCAGGTTAATCTGACAAAGGTGTTTCGCCAATCAGACCCTCAGCTTATCAAGTTACTTCATGGAATAAGAAGAGGAGAGAGTGATCCTGAAGACTTAAAGTTTCTTGAACAAGCTTGCTCGGAGGCTGAGCCAGATCCTTCTGTGCTACAACTCTATCCAAGGATTGAAGATGTGAATAGAGTGAATGCAAGTAGATTAGAAAGCTTGGGCAATGAACTTTTTGTCTATACAGCTGTTGATAGTGGTGAGGAGTCTAGGAAACCACAGCTTGAACAGGGAATAGCCCCCAAGAAAGTTACTTTGTGTAAAGATGCGAGGGTGATGCTGGTCAAGAATTTGAATACTTGGCGTGGCCTGGTGAATGGTGCGACTGGTACAGTTCAGGGATTTTATGTGCCCAAAAATAAGGATATAACAGGCATATGCCATGCTGCTCTACCGATAGTCAAGTTTGATTCAGGGCCAGAACTGTTGATTGAACCAGAAACTTGGGTTGTGACAGAGGGAGATAAGGTTGTTGCTGAACGGAAGCAGGTGCCTCTCATATTGGCATGGGCTTCAAGCATTCACAAGTGTCAGGGCATGACTCTTGACCGCCTTCACACTGATCTCTCTAAAGCTTTCGAGAATGGAATGGTTTATGTAGCTCTTTCCCGTGTGAGAAGCTTGGAAGGCCTGTATTTATCTGGTTTCGATCGCTGGAAGATTATGGTACACCCTAAGGTTTTGCAGTTCTATAACAGTTTAGGTGGTGAACTGGGTCAGGAGGGCAAGGATGATAATGATAGCCAGAGACAAGATAGCATGAACCGCAGTGGTAATGGCACTCAGAGACAGGATAGTACTAGCTGTAGTGGTAATGGCAGCCAGAGACAAAGTAGCACTAGGCGCAGTGGTAATGGCAACCAGAAAGATGATGGCAGCAAATACGTGCTGGTTCACATGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PIF1

Protein Analysis

543

Amino Acids

60.44

Weight (kDa)

9.4

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PIF1 PF05970 55 - 239 1.8e-43 PIF1-like helicase
AAA_30 PF13604 55 - 263 8.3e-16 AAA domain
DEAD PF00270 55 - 181 6.9e-07 DEAD/DEAH box helicase
AAA_19 PF13245 58 - 202 3.7e-15 AAA domain
AAA_22 PF13401 68 - 191 1.2e-06 AAA domain
Pif1_2B_dom PF21530 318 - 356 7.9e-07 DNA helicase Pif1, 2B domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017343)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g37811
prunus_persica Prupe.3G170200_v2.0.a1
pyrus_communis pycom09g07450 pycom17g13510
rosa_laevigata RLG00000020517
rosa_multiflora Rmu_sc0000185.1_g000025
rosa_roxburghii Rroxscaffold_2G00096840
rosa_rugosa Rorug02G0423300
rosa_samantha Rh2AG483700 Rh2BG496000 Rh2CG469900 Rh2DG507000
rosa_wichuraiana Rw2G039640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1201
Acc36I ACCTGC 2 cut(s) 670, 1201
AccB1I GGYRCC 1 cut(s) 1213
AciI CCGC 5 cut(s) 230, 329, 368, 1273, 1489
AclWI GGATC 2 cut(s) 755, 806
AcsI RAATTY 5 cut(s) 49, 153, 619, 661, 1012
AcuI CTGAAG 1 cut(s) 786
AfaI GTAC 3 cut(s) 1054, 1392, 1522
AfiI CCNNNNNNNGG 3 cut(s) 125, 345, 657
AgeI ACCGGT 1 cut(s) 221
AjnI CCWGG 2 cut(s) 90, 1032
Alw26I GTCTC 4 cut(s) 392, 1465, 1504, 1543
AlwI GGATC 2 cut(s) 755, 806
AoxI GGCC 5 cut(s) 86, 105, 1030, 1139, 1351
ApeKI GCWGC 5 cut(s) 283, 406, 1109, 1542, 1599
ApoI RAATTY 5 cut(s) 49, 153, 619, 661, 1012
AsiGI ACCGGT 1 cut(s) 221
AspLEI GCGC 2 cut(s) 316, 1569
AspS9I GGNCC 2 cut(s) 87, 1139
AsuHPI GGTGA 4 cut(s) 935, 1009, 1048, 1442
AxyI CCTNAGG 1 cut(s) 1398
BaeGI GKGCMC 2 cut(s) 236, 1077
BanI GGYRCC 1 cut(s) 1213
BbsI GAAGAC 1 cut(s) 774
BbvCI CCTCAGC 1 cut(s) 717
BbvI GCAGC 5 cut(s) 295, 393, 1096, 1554, 1611
BccI CCATC 2 cut(s) 153, 1589
BcgI CGANNNNNNTGC 2 cut(s) 982, 1016
BciT130I CCWGG 2 cut(s) 92, 1034
BciVI GTATCC 2 cut(s) 390, 484
BcoDI GTCTC 4 cut(s) 392, 1465, 1504, 1543
BfaI CTAG 4 cut(s) 666, 933, 1524, 1563
BfmI CTRYAG 1 cut(s) 1528
BfoI RGCGCY 1 cut(s) 317
BfuAI ACCTGC 2 cut(s) 670, 1201
BfuI GTATCC 2 cut(s) 390, 484
BisI GCNGC 6 cut(s) 284, 330, 407, 1110, 1543, 1600
BlpI GCTNAGC 1 cut(s) 804
BlsI GCNGC 6 cut(s) 285, 331, 408, 1111, 1544, 1601
BmcAI AGTACT 1 cut(s) 1522
Bme1390I CCNGG 2 cut(s) 92, 1034
BmgT120I GGNCC 2 cut(s) 87, 1139
BmiI GGNNCC 3 cut(s) 89, 292, 1215
BmrFI CCNGG 2 cut(s) 92, 1034
BmrI ACTGGG 1 cut(s) 1445
BmsI GCATC 2 cut(s) 979, 990
BmuI ACTGGG 1 cut(s) 1445
BpiI GAAGAC 1 cut(s) 774
Bpu10I CCTNAGC 1 cut(s) 717
Bpu1102I GCTNAGC 1 cut(s) 804
BsaAI YACGTR 1 cut(s) 1609
BsaI GGTCTC 1 cut(s) 392
BsaJI CCNNGG 3 cut(s) 90, 384, 836
BsaWI WCCGGW 1 cut(s) 221
BsaXI ACNNNNNCTCC 2 cut(s) 1176, 1206
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 345, 657
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 3 cut(s) 241, 1054, 1440
Bse21I CCTNAGG 1 cut(s) 1398
Bse3DI GCAATG 2 cut(s) 519, 895
BseBI CCWGG 2 cut(s) 92, 1034
BseDI CCNNGG 3 cut(s) 90, 384, 836
BseGI GGATG 2 cut(s) 478, 1459
BseLI CCNNNNNNNGG 3 cut(s) 125, 345, 657
BseMI GCAATG 2 cut(s) 519, 895
BseMII CTCAG 4 cut(s) 486, 731, 795, 1520
BseNI ACTGG 3 cut(s) 241, 1054, 1440
BseRI GAGGAG 2 cut(s) 765, 941
BseSI GKGCMC 2 cut(s) 236, 1077
BseXI GCAGC 5 cut(s) 295, 393, 1096, 1554, 1611
BseYI CCCAGC 1 cut(s) 612
BsgI GTGCAG 1 cut(s) 24
Bsh1285I CGRYCG 1 cut(s) 1375
BshFI GGCC 5 cut(s) 88, 107, 1032, 1141, 1353
BshNI GGYRCC 1 cut(s) 1213
BshTI ACCGGT 1 cut(s) 221
BsiEI CGRYCG 1 cut(s) 1375
BsiSI CCGG 1 cut(s) 222
BslI CCNNNNNNNGG 3 cut(s) 125, 345, 657
BsmAI GTCTC 4 cut(s) 392, 1465, 1504, 1543
BsmI GAATGC 2 cut(s) 868, 1242
BsnI GGCC 5 cut(s) 88, 107, 1032, 1141, 1353
Bso31I GGTCTC 1 cut(s) 392
Bsp1286I GDGCHC 2 cut(s) 236, 1077
Bsp143I GATC 4 cut(s) 760, 811, 1285, 1372
Bsp1720I GCTNAGC 1 cut(s) 804
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 5 cut(s) 230, 329, 368, 1273, 1489
BspANI GGCC 5 cut(s) 88, 107, 1032, 1141, 1353
BspCNI CTCAG 4 cut(s) 487, 730, 796, 1519
BspLI GGNNCC 3 cut(s) 89, 292, 1215
BspMI ACCTGC 2 cut(s) 670, 1201
BspPI GGATC 2 cut(s) 755, 806
BspT107I GGYRCC 1 cut(s) 1213
BspTNI GGTCTC 1 cut(s) 392
BsrDI GCAATG 2 cut(s) 519, 895
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 3 cut(s) 241, 1054, 1440
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 3 cut(s) 90, 384, 836
BssMI GATC 4 cut(s) 760, 811, 1285, 1372
BssT1I CCWWGG 2 cut(s) 384, 836
Bst2UI CCWGG 2 cut(s) 92, 1034
Bst4CI ACNGT 3 cut(s) 1057, 1149, 1421
Bst6I CTCTTC 2 cut(s) 360, 742
BstBAI YACGTR 1 cut(s) 1609
BstC8I GCNNGC 2 cut(s) 232, 793
BstDEI CTNAG 6 cut(s) 297, 495, 717, 804, 1398, 1506
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 2 cut(s) 478, 1459
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 2 cut(s) 316, 1569
BstKTI GATC 4 cut(s) 763, 814, 1288, 1375
BstMAI GTCTC 4 cut(s) 392, 1465, 1504, 1543
BstMBI GATC 4 cut(s) 760, 811, 1285, 1372
BstMCI CGRYCG 1 cut(s) 1375
BstMWI GCNNNNNNNGC 5 cut(s) 335, 647, 1350, 1488, 1566
BstNI CCWGG 2 cut(s) 92, 1034
BstSCI CCNGG 2 cut(s) 90, 1032
BstSFI CTRYAG 1 cut(s) 1528
BstSLI GKGCMC 2 cut(s) 236, 1077
BstV1I GCAGC 5 cut(s) 295, 393, 1096, 1554, 1611
BstV2I GAAGAC 1 cut(s) 774
BstX2I RGATCY 1 cut(s) 811
BstXI CCANNNNNNTGG 1 cut(s) 1167
BstYI RGATCY 1 cut(s) 811
Bsu36I CCTNAGG 1 cut(s) 1398
BsuI GTATCC 2 cut(s) 390, 484
BsuRI GGCC 5 cut(s) 88, 107, 1032, 1141, 1353
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 2 cut(s) 478, 1459
BtsI GCAGTG 2 cut(s) 1498, 1576
BtsIMutI CAGTG 4 cut(s) 234, 1281, 1498, 1576
BveI ACCTGC 2 cut(s) 670, 1201
Cac8I GCNNGC 2 cut(s) 232, 793
CfoI GCGC 2 cut(s) 316, 1569
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 2 cut(s) 87, 1139
Csp6I GTAC 3 cut(s) 1053, 1391, 1521
CspAI ACCGGT 1 cut(s) 221
CviAII CATG 9 cut(s) 287, 385, 484, 737, 1106, 1230, 1261, 1483, 1621
CviQI GTAC 3 cut(s) 1053, 1391, 1521
DdeI CTNAG 6 cut(s) 297, 495, 717, 804, 1398, 1506
DpnI GATC 4 cut(s) 762, 813, 1287, 1374
DpnII GATC 4 cut(s) 760, 811, 1285, 1372
Eam1104I CTCTTC 2 cut(s) 360, 742
EarI CTCTTC 2 cut(s) 360, 742
Eco130I CCWWGG 2 cut(s) 384, 836
Eco147I AGGCCT 1 cut(s) 1353
Eco31I GGTCTC 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 786
Eco81I CCTNAGG 1 cut(s) 1398
EcoRI GAATTC 2 cut(s) 619, 661
EcoRII CCWGG 2 cut(s) 90, 1032
EcoT14I CCWWGG 2 cut(s) 384, 836
EcoT22I ATGCAT 1 cut(s) 643
ErhI CCWWGG 2 cut(s) 384, 836
FaeI CATG 9 cut(s) 290, 388, 487, 740, 1109, 1233, 1264, 1486, 1624
FatI CATG 9 cut(s) 286, 384, 483, 736, 1105, 1229, 1260, 1482, 1620
FauI CCCGC 2 cut(s) 223, 361
FauNDI CATATG 1 cut(s) 1100
Fnu4HI GCNGC 6 cut(s) 284, 330, 407, 1110, 1543, 1600
FokI GGATG 2 cut(s) 485, 1466
Fsp4HI GCNGC 6 cut(s) 284, 330, 407, 1110, 1543, 1600
FspBI CTAG 4 cut(s) 666, 933, 1524, 1563
GlaI GCGC 2 cut(s) 315, 1568
GluI GCNGC 6 cut(s) 284, 330, 407, 1110, 1543, 1600
GsaI CCCAGC 1 cut(s) 616
HaeII RGCGCY 1 cut(s) 317
HaeIII GGCC 5 cut(s) 88, 107, 1032, 1141, 1353
HapII CCGG 1 cut(s) 222
HhaI GCGC 2 cut(s) 316, 1569
Hin1II CATG 9 cut(s) 290, 388, 487, 740, 1109, 1233, 1264, 1486, 1624
Hin6I GCGC 2 cut(s) 314, 1567
HinP1I GCGC 2 cut(s) 314, 1567
HindIII AAGCTT 4 cut(s) 789, 880, 1296, 1342
HinfI GANTC 6 cut(s) 352, 508, 562, 929, 1133, 1264
HpaII CCGG 1 cut(s) 222
HphI GGTGA 4 cut(s) 935, 1009, 1048, 1442
Hpy166II GTNNAC 3 cut(s) 1393, 1433, 1618
Hpy188I TCNGA 6 cut(s) 269, 567, 690, 712, 799, 1509
Hpy188III TCNNGA 8 cut(s) 505, 512, 764, 782, 1009, 1268, 1303, 1442
Hpy8I GTNNAC 3 cut(s) 1393, 1433, 1618
HpyAV CCTTC 4 cut(s) 445, 825, 1286, 1343
HpyCH4III ACNGT 3 cut(s) 1057, 1149, 1421
HpyCH4IV ACGT 1 cut(s) 1608
HpyCH4V TGCA 6 cut(s) 41, 409, 641, 679, 866, 1408
HpyF10VI GCNNNNNNNGC 5 cut(s) 335, 647, 1350, 1488, 1566
HpyF3I CTNAG 6 cut(s) 297, 495, 717, 804, 1398, 1506
HpySE526I ACGT 1 cut(s) 1608
Hsp92II CATG 9 cut(s) 290, 388, 487, 740, 1109, 1233, 1264, 1486, 1624
HspAI GCGC 2 cut(s) 314, 1567
Kzo9I GATC 4 cut(s) 760, 811, 1285, 1372
LmnI GCTCC 1 cut(s) 290
Lsp1109I GCAGC 5 cut(s) 295, 393, 1096, 1554, 1611
LweI GCATC 2 cut(s) 979, 990
MaeI CTAG 4 cut(s) 666, 933, 1524, 1563
MaeII ACGT 1 cut(s) 1608
MaeIII GTNAC 6 cut(s) 68, 307, 594, 729, 973, 1174
MalI GATC 4 cut(s) 762, 813, 1287, 1374
MboI GATC 4 cut(s) 760, 811, 1285, 1372
MboII GAAGA 5 cut(s) 347, 759, 779, 857, 1393
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 2 cut(s) 236, 1077
MluCI AATT 7 cut(s) 49, 63, 153, 246, 619, 661, 1012
MlyI GAGTC 4 cut(s) 346, 517, 938, 1258
MmeI TCCRAC 1 cut(s) 638
Mph1103I ATGCAT 1 cut(s) 643
MseI TTAA 4 cut(s) 456, 606, 684, 773
MspA1I CMGCKG 2 cut(s) 230, 911
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 2 cut(s) 92, 1034
Mva1269I GAATGC 2 cut(s) 868, 1242
MvaI CCWGG 2 cut(s) 92, 1034
MwoI GCNNNNNNNGC 5 cut(s) 335, 647, 1350, 1488, 1566
NcoI CCATGG 1 cut(s) 384
NdeI CATATG 1 cut(s) 1100
NdeII GATC 4 cut(s) 760, 811, 1285, 1372
NlaIII CATG 9 cut(s) 290, 388, 487, 740, 1109, 1233, 1264, 1486, 1624
NlaIV GGNNCC 3 cut(s) 89, 292, 1215
NmuCI GTSAC 2 cut(s) 307, 1174
NsiI ATGCAT 1 cut(s) 643
PaqCI CACCTGC 1 cut(s) 1201
PceI AGGCCT 1 cut(s) 1353
PctI GAATGC 2 cut(s) 868, 1242
PfeI GAWTC 2 cut(s) 562, 1133
PinAI ACCGGT 1 cut(s) 221
PkrI GCNGC 6 cut(s) 285, 331, 408, 1111, 1544, 1601
Ple19I CGATCG 1 cut(s) 1375
PleI GAGTC 4 cut(s) 346, 516, 937, 1258
PpsI GAGTC 4 cut(s) 346, 516, 937, 1258
Ppu21I YACGTR 1 cut(s) 1609
Psp6I CCWGG 2 cut(s) 90, 1032
PspFI CCCAGC 1 cut(s) 612
PspGI CCWGG 2 cut(s) 90, 1032
PspN4I GGNNCC 3 cut(s) 89, 292, 1215
PspPI GGNCC 2 cut(s) 87, 1139
PsuI RGATCY 1 cut(s) 811
PvuI CGATCG 1 cut(s) 1375
PvuII CAGCTG 1 cut(s) 911
RsaI GTAC 3 cut(s) 1054, 1392, 1522
RsaNI GTAC 3 cut(s) 1053, 1391, 1521
SaqAI TTAA 4 cut(s) 456, 606, 684, 773
SatI GCNGC 6 cut(s) 284, 330, 407, 1110, 1543, 1600
Sau3AI GATC 4 cut(s) 760, 811, 1285, 1372
Sau96I GGNCC 2 cut(s) 87, 1139
ScaI AGTACT 1 cut(s) 1522
SchI GAGTC 4 cut(s) 346, 517, 938, 1258
ScrFI CCNGG 2 cut(s) 92, 1034
SduI GDGCHC 2 cut(s) 236, 1077
SfaNI GCATC 2 cut(s) 979, 990
SfcI CTRYAG 1 cut(s) 1528
Sse9I AATT 7 cut(s) 49, 63, 153, 246, 619, 661, 1012
SseBI AGGCCT 1 cut(s) 1353
SsiI CCGC 5 cut(s) 230, 329, 368, 1273, 1489
SspMI CTAG 4 cut(s) 666, 933, 1524, 1563
StuI AGGCCT 1 cut(s) 1353
StyD4I CCNGG 2 cut(s) 90, 1032
StyI CCWWGG 2 cut(s) 384, 836
TaaI ACNGT 3 cut(s) 1057, 1149, 1421
TaiI ACGT 1 cut(s) 1611
TaqI TCGA 4 cut(s) 157, 504, 1302, 1371
TasI AATT 7 cut(s) 49, 63, 153, 246, 619, 661, 1012
TatI WGTACW 1 cut(s) 1520
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 2 cut(s) 562, 1133
Tru1I TTAA 4 cut(s) 456, 606, 684, 773
Tru9I TTAA 4 cut(s) 456, 606, 684, 773
TscAI CASTG 4 cut(s) 241, 1288, 1498, 1576
TseFI GTSAC 2 cut(s) 307, 1174
TseI GCWGC 5 cut(s) 283, 406, 1109, 1542, 1599
Tsp45I GTSAC 2 cut(s) 307, 1174
TspDTI ATGAA 6 cut(s) 29, 183, 205, 725, 906, 1499
TspGWI ACGGA 1 cut(s) 1219
TspRI CASTG 4 cut(s) 241, 1288, 1498, 1576
XapI RAATTY 5 cut(s) 49, 153, 619, 661, 1012
XcmI CCANNNNNNNNNTGG 1 cut(s) 1592
XspI CTAG 4 cut(s) 666, 933, 1524, 1563
ZrmI AGTACT 1 cut(s) 1522
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.