FvH4_6g38350

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016898
Physical Location & Seq
Forward (+)
30310670 .. 30312432
1763 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38350.t1

Sequence Viewer

Length: 1212 bp
ATGGATGATGAGCCTTTTCTTTCCAAGATTGCAGCAGAGGTAGGTGGTGAAGAAGGGACTAGCAGAGCAACACCAAGAAGATTATCAAGAGACTTCCGCTCGAGTTTCCTTGATCTCGACCCTTCGCGCCGTGTCTCAAGTACTCAGCTGATCACAAGTGCTGGTGATGTTATTGTTCCCATAACTCCCAGTTCTTCTTCATATGTAAATCTCATTGCCAATTTGAACAAGAACAGGAAAAGAAGGCTCAAACGTCGATCACACTCGGCACCCTCGGTGTTCACCGACTTCAAGGAGACGATACAAGATTCTCTGGATCCCAGACCTGCCCCTAAATCAACCCCCAACATTGTTAGGCAAGCCTCCATCGGTGTTGTAGTGTATATCATAATTGGCATTGTGATATACTTGACCACCGGAGGTATCTCAGGGGAGGATGTCACATACCGGCCAGTGGACGCCATGTACTTCATTGTTGTCACGCTCTGCACCATTGGATATGGTGACATTGTTCCAGACACAACATTTACCAAACTCTTCACTTGTTTCTTCATCTTGGTTGGTTTCGGTTTCATTGATATTCTGCTACATGGGTTGGTGACCTACATCTGTGATAGGCAAGAATCCGTTTTGTTGAGCACGGTGGATGAGAATAAGTTCAACACAATGGTTCAGGCGTATTTCATTGATAAAGAGAAGGGAAGGATGAGAATACGGATAAAGGTATGTTTGGCCTTGACAGTAGTCTTTTGTTGTATAACTATAGGCACAATAGTAGCTCATTATGTAGAGGGCTACAGCTGGGTTGATAGTTTTTACCTATCTGTTACATCAGTGACAACAGTGGGTTATGGGGATTATGCTTTCAAAACCGTAGGAGGAAGATGTTTTGCAGTAGTCTGGTTATTAGTAAGCACATTCGCCGTTGCACGGGCGTTTTTGTACTTGACAGAGATGAGAATCGACAAGAGAAATCGCAAAATTGCGAAATGGGTTCTTCAAAAGGAGGTCACCCTAAGGGATTTACTGGCAGCAGACCTGGATAATGATGGATGCATCAGCAAATCAGAGTTCGTCTTGTACAAGCTCAAGGAGATGGGCATGGTAGCTAACAAGGACATCCTTCTGATATGCAAACAATTTGATTCTTTGGGGCATAGTAATGATGGCAAGATTACTCTTGTTGACCTAATGGAAGGTGACAATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

404

Amino Acids

45.03

Weight (kDa)

7.98

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 122 - 196 2.9e-06 Ion transport protein
Ion_trans_2 PF07885 125 - 201 3.3e-13 Ion channel
Ion_trans_2 PF07885 248 - 318 1.6e-12 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016898)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g38350
malus_domestica MD09G1150900.v1.1
prunus_persica Prupe.3G174100_v2.0.a1
pyrus_communis pycom09g07070 pycom17g13150
rosa_chinensis RchiOBHm_Chr2g0151661
rosa_multiflora Rmu_sc0005294.1_g000051
rosa_roxburghii Rroxscaffold_2G00096280
rosa_rugosa Rorug02G0427600
rosa_samantha Rh2AG488500 Rh2BG501200 Rh2CG475000 Rh2DG512300
rosa_wichuraiana Rw2G040060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 334
AccB1I GGYRCC 1 cut(s) 268
AccBSI CCGCTC 1 cut(s) 99
AccII CGCG 1 cut(s) 127
AciI CCGC 1 cut(s) 97
AclWI GGATC 2 cut(s) 311, 324
AcoI YGGCCR 1 cut(s) 449
AcyI GRCGYC 1 cut(s) 459
AfaI GTAC 4 cut(s) 142, 467, 944, 1082
AfiI CCNNNNNNNGG 2 cut(s) 454, 930
AgsI TTSAA 5 cut(s) 226, 292, 661, 868, 1001
AjnI CCWGG 1 cut(s) 1038
AluBI AGCT 5 cut(s) 148, 779, 801, 1087, 1109
AluI AGCT 5 cut(s) 148, 779, 801, 1087, 1109
Alw21I GWGCWC 1 cut(s) 641
Alw26I GTCTC 3 cut(s) 84, 139, 290
AlwI GGATC 2 cut(s) 311, 324
Ama87I CYCGRG 1 cut(s) 100
AoxI GGCC 2 cut(s) 449, 732
ApeKI GCWGC 2 cut(s) 32, 1031
Asp700I GAANNNNTTC 1 cut(s) 656
AspLEI GCGC 1 cut(s) 129
AsuHPI GGTGA 7 cut(s) 59, 176, 274, 515, 610, 1003, 1211
AvaI CYCGRG 1 cut(s) 100
AxyI CCTNAGG 1 cut(s) 1016
BamHI GGATCC 1 cut(s) 316
BanI GGYRCC 1 cut(s) 268
Bbv12I GWGCWC 1 cut(s) 641
BbvI GCAGC 2 cut(s) 44, 1043
BccI CCATC 4 cut(s) 374, 1043, 1090, 1160
BceAI ACGGC 2 cut(s) 114, 908
BciT130I CCWGG 1 cut(s) 1040
BclI TGATCA 1 cut(s) 150
BcoDI GTCTC 3 cut(s) 84, 139, 290
BfaI CTAG 1 cut(s) 60
BfmI CTRYAG 2 cut(s) 762, 796
BfuAI ACCTGC 1 cut(s) 334
BisI GCNGC 2 cut(s) 33, 1032
BlsI GCNGC 2 cut(s) 34, 1033
BmcAI AGTACT 1 cut(s) 142
Bme1390I CCNGG 1 cut(s) 1040
BmeT110I CYCGRG 1 cut(s) 100
BmiI GGNNCC 2 cut(s) 270, 318
BmrFI CCNGG 1 cut(s) 1040
BmrI ACTGGG 1 cut(s) 183
BmsI GCATC 2 cut(s) 1043, 1065
BmuI ACTGGG 1 cut(s) 183
BoxI GACNNNNGTC 1 cut(s) 743
BpuEI CTTGAG 2 cut(s) 121, 1073
BsaBI GATNNNNATC 1 cut(s) 959
BsaHI GRCGYC 1 cut(s) 459
BsaJI CCNNGG 1 cut(s) 273
BsaWI WCCGGW 1 cut(s) 416
Bsc4I CCNNNNNNNGG 2 cut(s) 454, 930
Bse118I RCCGGY 1 cut(s) 447
Bse1I ACTGG 3 cut(s) 189, 452, 1032
Bse21I CCTNAGG 1 cut(s) 1016
Bse3DI GCAATG 1 cut(s) 213
Bse8I GATNNNNATC 1 cut(s) 959
BseBI CCWGG 1 cut(s) 1040
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 6 cut(s) 10, 442, 652, 711, 1058, 1119
BseJI GATNNNNATC 1 cut(s) 959
BseLI CCNNNNNNNGG 2 cut(s) 454, 930
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 2 cut(s) 158, 441
BseNI ACTGG 3 cut(s) 189, 452, 1032
BseXI GCAGC 2 cut(s) 44, 1043
BseYI CCCAGC 1 cut(s) 801
BsgI GTGCAG 1 cut(s) 472
Bsh1236I CGCG 1 cut(s) 127
BshFI GGCC 2 cut(s) 451, 734
BshNI GGYRCC 1 cut(s) 268
BsiHKAI GWGCWC 1 cut(s) 641
BsiHKCI CYCGRG 1 cut(s) 100
BsiSI CCGG 2 cut(s) 417, 448
BslFI GGGAC 1 cut(s) 70
BslI CCNNNNNNNGG 2 cut(s) 454, 930
BsmAI GTCTC 3 cut(s) 84, 139, 290
BsmBI CGTCTC 1 cut(s) 290
BsmFI GGGAC 1 cut(s) 70
BsnI GGCC 2 cut(s) 451, 734
BsoBI CYCGRG 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 641
Bsp1407I TGTACA 1 cut(s) 1080
Bsp143I GATC 4 cut(s) 112, 150, 257, 316
BspACI CCGC 1 cut(s) 97
BspANI GGCC 2 cut(s) 451, 734
BspCNI CTCAG 2 cut(s) 157, 440
BspFNI CGCG 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 270, 318
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 2 cut(s) 311, 324
BspT107I GGYRCC 1 cut(s) 268
BsrBI CCGCTC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 213
BsrFI RCCGGY 1 cut(s) 447
BsrGI TGTACA 1 cut(s) 1080
BsrI ACTGG 3 cut(s) 189, 452, 1032
BssAI RCCGGY 1 cut(s) 447
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 4 cut(s) 112, 150, 257, 316
BssNI GRCGYC 1 cut(s) 459
Bst2UI CCWGG 1 cut(s) 1040
Bst4CI ACNGT 4 cut(s) 643, 742, 844, 874
Bst6I CTCTTC 1 cut(s) 542
BstACI GRCGYC 1 cut(s) 459
BstAUI TGTACA 1 cut(s) 1080
BstC8I GCNNGC 1 cut(s) 360
BstDEI CTNAG 3 cut(s) 144, 427, 1016
BstEII GGTNACC 2 cut(s) 598, 1009
BstF5I GGATG 6 cut(s) 10, 442, 652, 711, 1058, 1119
BstFNI CGCG 1 cut(s) 127
BstHHI GCGC 1 cut(s) 129
BstKTI GATC 4 cut(s) 115, 153, 260, 319
BstMAI GTCTC 3 cut(s) 84, 139, 290
BstMBI GATC 4 cut(s) 112, 150, 257, 316
BstNI CCWGG 1 cut(s) 1040
BstPAI GACNNNNGTC 1 cut(s) 743
BstPI GGTNACC 2 cut(s) 598, 1009
BstSCI CCNGG 1 cut(s) 1038
BstSFI CTRYAG 2 cut(s) 762, 796
BstUI CGCG 1 cut(s) 127
BstV1I GCAGC 2 cut(s) 44, 1043
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
Bsu36I CCTNAGG 1 cut(s) 1016
BsuRI GGCC 2 cut(s) 451, 734
BtsCI GGATG 6 cut(s) 10, 442, 652, 711, 1058, 1119
BtsIMutI CAGTG 3 cut(s) 459, 840, 849
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 1 cut(s) 360
CfoI GCGC 1 cut(s) 129
Cfr10I RCCGGY 1 cut(s) 447
CseI GACGC 1 cut(s) 467
Csp6I GTAC 4 cut(s) 141, 466, 943, 1081
CviAII CATG 3 cut(s) 463, 590, 1102
CviQI GTAC 4 cut(s) 141, 466, 943, 1081
DdeI CTNAG 3 cut(s) 144, 427, 1016
DpnI GATC 4 cut(s) 114, 152, 259, 318
DpnII GATC 4 cut(s) 112, 150, 257, 316
EaeI YGGCCR 1 cut(s) 449
Eam1104I CTCTTC 1 cut(s) 542
EarI CTCTTC 1 cut(s) 542
Eco81I CCTNAGG 1 cut(s) 1016
Eco88I CYCGRG 1 cut(s) 100
Eco91I GGTNACC 2 cut(s) 598, 1009
EcoO65I GGTNACC 2 cut(s) 598, 1009
EcoRII CCWGG 1 cut(s) 1038
EcoT22I ATGCAT 1 cut(s) 1058
Esp3I CGTCTC 1 cut(s) 290
FaeI CATG 3 cut(s) 466, 593, 1105
FaqI GGGAC 1 cut(s) 70
FatI CATG 3 cut(s) 462, 589, 1101
FauNDI CATATG 1 cut(s) 202
FbaI TGATCA 1 cut(s) 150
Fnu4HI GCNGC 2 cut(s) 33, 1032
FokI GGATG 6 cut(s) 17, 449, 659, 718, 1065, 1106
Fsp4HI GCNGC 2 cut(s) 33, 1032
FspBI CTAG 1 cut(s) 60
GlaI GCGC 1 cut(s) 128
GluI GCNGC 2 cut(s) 33, 1032
GsaI CCCAGC 1 cut(s) 805
HaeIII GGCC 2 cut(s) 451, 734
HapII CCGG 2 cut(s) 417, 448
HgaI GACGC 1 cut(s) 467
HhaI GCGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 3 cut(s) 466, 593, 1105
Hin6I GCGC 1 cut(s) 127
HinP1I GCGC 1 cut(s) 127
HincII GTYRAC 1 cut(s) 1186
HindII GTYRAC 1 cut(s) 1186
HinfI GANTC 4 cut(s) 308, 623, 960, 1145
HpaII CCGG 2 cut(s) 417, 448
HphI GGTGA 7 cut(s) 59, 176, 274, 515, 610, 1003, 1211
Hpy166II GTNNAC 3 cut(s) 282, 457, 1186
Hpy188I TCNGA 2 cut(s) 1069, 1128
Hpy188III TCNNGA 4 cut(s) 87, 116, 314, 515
Hpy8I GTNNAC 3 cut(s) 282, 457, 1186
Hpy99I CGWCG 1 cut(s) 258
HpyAV CCTTC 7 cut(s) 47, 132, 237, 691, 696, 1133, 1190
HpyCH4III ACNGT 4 cut(s) 643, 742, 844, 874
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 6 cut(s) 32, 489, 893, 929, 1056, 1134
HpyF3I CTNAG 3 cut(s) 144, 427, 1016
HpySE526I ACGT 1 cut(s) 253
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 3 cut(s) 466, 593, 1105
HspAI GCGC 1 cut(s) 127
Ksp22I TGATCA 1 cut(s) 150
Kzo9I GATC 4 cut(s) 112, 150, 257, 316
Lsp1109I GCAGC 2 cut(s) 44, 1043
LweI GCATC 2 cut(s) 1043, 1065
MaeI CTAG 1 cut(s) 60
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 8 cut(s) 439, 478, 503, 598, 826, 835, 1009, 1199
MalI GATC 4 cut(s) 114, 152, 259, 318
MbiI CCGCTC 1 cut(s) 99
MboI GATC 4 cut(s) 112, 150, 257, 316
MboII GAAGA 8 cut(s) 62, 90, 186, 189, 529, 541, 894, 989
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 1 cut(s) 641
MluCI AATT 4 cut(s) 220, 390, 981, 1139
MnlI CCTC 8 cut(s) 31, 283, 373, 413, 427, 784, 872, 1000
Mph1103I ATGCAT 1 cut(s) 1058
MroXI GAANNNNTTC 1 cut(s) 656
MslI CAYNNNNRTG 1 cut(s) 1161
MspA1I CMGCKG 2 cut(s) 148, 801
MspI CCGG 2 cut(s) 417, 448
MspR9I CCNGG 1 cut(s) 1040
MvaI CCWGG 1 cut(s) 1040
MvnI CGCG 1 cut(s) 127
NdeI CATATG 1 cut(s) 202
NdeII GATC 4 cut(s) 112, 150, 257, 316
NlaIII CATG 3 cut(s) 466, 593, 1105
NlaIV GGNNCC 2 cut(s) 270, 318
NmeAIII GCCGAG 1 cut(s) 245
NmuCI GTSAC 7 cut(s) 439, 478, 503, 598, 835, 1009, 1199
NsiI ATGCAT 1 cut(s) 1058
PaeR7I CTCGAG 1 cut(s) 100
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 4 cut(s) 308, 623, 960, 1145
PkrI GCNGC 2 cut(s) 34, 1033
PshAI GACNNNNGTC 1 cut(s) 743
Psp6I CCWGG 1 cut(s) 1038
PspEI GGTNACC 2 cut(s) 598, 1009
PspFI CCCAGC 1 cut(s) 801
PspGI CCWGG 1 cut(s) 1038
PspN4I GGNNCC 2 cut(s) 270, 318
PspXI VCTCGAGB 1 cut(s) 100
PsuI RGATCY 1 cut(s) 316
PvuII CAGCTG 2 cut(s) 148, 801
RsaI GTAC 4 cut(s) 142, 467, 944, 1082
RsaNI GTAC 4 cut(s) 141, 466, 943, 1081
RseI CAYNNNNRTG 1 cut(s) 1161
SatI GCNGC 2 cut(s) 33, 1032
Sau3AI GATC 4 cut(s) 112, 150, 257, 316
ScaI AGTACT 1 cut(s) 142
ScrFI CCNGG 1 cut(s) 1040
SduI GDGCHC 1 cut(s) 641
SfaNI GCATC 2 cut(s) 1043, 1065
SfcI CTRYAG 2 cut(s) 762, 796
Sfr274I CTCGAG 1 cut(s) 100
SlaI CTCGAG 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 1161
SmlI CTYRAG 3 cut(s) 100, 136, 1088
SmoI CTYRAG 3 cut(s) 100, 136, 1088
Sse9I AATT 4 cut(s) 220, 390, 981, 1139
SsiI CCGC 1 cut(s) 97
SspMI CTAG 1 cut(s) 60
StyD4I CCNGG 1 cut(s) 1038
TaaI ACNGT 4 cut(s) 643, 742, 844, 874
TaiI ACGT 1 cut(s) 256
TaqI TCGA 4 cut(s) 101, 117, 256, 963
TasI AATT 4 cut(s) 220, 390, 981, 1139
TatI WGTACW 4 cut(s) 140, 465, 942, 1080
TfiI GAWTC 4 cut(s) 308, 623, 960, 1145
TscAI CASTG 3 cut(s) 459, 840, 849
TseFI GTSAC 7 cut(s) 439, 478, 503, 598, 835, 1009, 1199
TseI GCWGC 2 cut(s) 32, 1031
Tsp45I GTSAC 7 cut(s) 439, 478, 503, 598, 835, 1009, 1199
TspDTI ATGAA 5 cut(s) 189, 460, 541, 562, 673
TspGWI ACGGA 2 cut(s) 616, 730
TspRI CASTG 3 cut(s) 459, 840, 849
XhoI CTCGAG 1 cut(s) 100
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 1 cut(s) 60
ZrmI AGTACT 1 cut(s) 142
Zsp2I ATGCAT 1 cut(s) 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.