Rh2CG475000

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0016898
Physical Location & Seq
Forward (+)
64031563 .. 64032979
1417 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG475000.1

Sequence Viewer

Length: 1209 bp
ATGGATGATCAGCCTTTTCTTTCCAAGCTTGCAGCAGAGGCAGCTGGAGAAGGGACTAGCAGGGCAGCACCAAGAAAATCATCAAGAGACTTATTTCACTCAAGTTTCCTTGATCTTGGCCCTGCACGCCGCCTCTCAAGTACTCAGCTAATCACAAGTGCTGGTGATGTTGTTGTTCCCATAACACCAAATTCTTCTTCCTATGCAAATCTCATTGCCAACTTGAACAAGAACAGGAAAAGAAGGCTCAAACGTCGATCACACTCAGCTCCATCAGTGTTCACCGACTGTAAGGAGACGATTCCAGATTCTTTGGATCCCAGACCTGCCCCTAAATCAACCCCTAACATTGTTAGGCAAGCCTTCATCGGTGTCATTGTATATATCATAATTGGCGTTGTGGTCTACTTGACCAGCGGTGGTATCTCAGGGGATGTTGTCACATACAAGCCAGTGGACGCCATGTACTTTATTGTTGTCACGCTGTGCACCATTGGATATGGTGACATTGTCCCGGACACAACATTTACCAAACTCTTCACTTGTTTCTTCATCTTGGTTGGTTTTGGTTTCATTGATATTCTGCTACATGGGTTGGTGACCTACATCTGTGACAGGCAAGAATCGGTTTTGTTGAGCACGGTGGATGAGAATAAGTTCAATACAATGGTTCAGGCGTATTTTATTGATAAAGAGAAGGGGAGGATGAGAATAAGGATCAAGGTATGCTTGGCCTTAACAGTAGTTTTTTGTTGTATAGCTATAGGCACAATAGTAGCTCATTATGCAGAGGGCTACAGCTGGGTTGATAGTTTTTACCTATCAGTTACATCAGTGACAACAGTAGGTTATGGGGATTATGCTTTCAAAACCATTGGAGGAAGATGTTTTGCAGTAGTCTGGTTATTAGTAAGCACATTCGCCGTCGCACGGGCGTTTTTGTACCTGACAGAGATGAGAATCGACAAGAGAAATCGCAAGATTGCGAAATGGGTTCTTCAAAAGGAGGTCACCCTAAGGGATTTACTGGCAGCAGACCTGGATAACGATGGATGTATCAGCAAATCAGAGTTCGTCTTGTACAAGCTGAAGGAGATGGGGATGGTAGCTAATAAGGACATCCTGCTGATTTGCAAACAATTTGATTCTTTGGGGCATAGCAATGATGGCAAGATTACTCTTGTTGACCTAATGGAAGGTGATAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

402

Amino Acids

44.56

Weight (kDa)

8.68

Isoelectric Point (pI)

32.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 120 - 196 1.2e-06 Ion transport protein
Ion_trans_2 PF07885 125 - 201 8.2e-13 Ion channel
Ion_trans_2 PF07885 248 - 318 8.3e-13 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016898)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g38350
malus_domestica MD09G1150900.v1.1
prunus_persica Prupe.3G174100_v2.0.a1
pyrus_communis pycom09g07070 pycom17g13150
rosa_chinensis RchiOBHm_Chr2g0151661
rosa_multiflora Rmu_sc0005294.1_g000051
rosa_roxburghii Rroxscaffold_2G00096280
rosa_rugosa Rorug02G0427600
rosa_samantha Rh2AG488500 Rh2BG501200 Rh2CG475000 Rh2DG512300
rosa_wichuraiana Rw2G040060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 334
AccI GTMKAC 1 cut(s) 405
AciI CCGC 2 cut(s) 130, 417
AclWI GGATC 3 cut(s) 311, 324, 725
AcsI RAATTY 1 cut(s) 190
AcuI CTGAAG 1 cut(s) 1109
AcyI GRCGYC 1 cut(s) 459
AdeI CACNNNGTG 1 cut(s) 486
AfaI GTAC 4 cut(s) 142, 467, 944, 1082
AfiI CCNNNNNNNGG 1 cut(s) 930
AgsI TTSAA 4 cut(s) 226, 661, 868, 1001
AjnI CCWGG 1 cut(s) 1038
AjuI GAANNNNNNNTTGG 2 cut(s) 181, 213
AluBI AGCT 9 cut(s) 28, 44, 148, 269, 761, 779, 801, 1087, 1109
AluI AGCT 9 cut(s) 28, 44, 148, 269, 761, 779, 801, 1087, 1109
Alw21I GWGCWC 2 cut(s) 491, 641
Alw26I GTCTC 2 cut(s) 81, 290
Alw44I GTGCAC 1 cut(s) 487
AlwI GGATC 3 cut(s) 311, 324, 725
AoxI GGCC 2 cut(s) 118, 732
ApaLI GTGCAC 1 cut(s) 487
ApeKI GCWGC 4 cut(s) 32, 41, 65, 1031
ApoI RAATTY 1 cut(s) 190
Asp700I GAANNNNTTC 1 cut(s) 656
AspS9I GGNCC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 515
AsuHPI GGTGA 5 cut(s) 176, 274, 515, 610, 1003
AxyI CCTNAGG 1 cut(s) 1016
BaeGI GKGCMC 1 cut(s) 491
BamHI GGATCC 1 cut(s) 316
Bbv12I GWGCWC 2 cut(s) 491, 641
BbvI GCAGC 4 cut(s) 44, 53, 77, 1043
BccI CCATC 5 cut(s) 280, 1043, 1090, 1096, 1160
BceAI ACGGC 1 cut(s) 908
BciT130I CCWGG 1 cut(s) 1040
BclI TGATCA 1 cut(s) 7
BcnI CCSGG 1 cut(s) 515
BcoDI GTCTC 2 cut(s) 81, 290
BfaI CTAG 1 cut(s) 57
BfmI CTRYAG 2 cut(s) 762, 796
BfuAI ACCTGC 1 cut(s) 334
BisI GCNGC 5 cut(s) 33, 42, 66, 130, 1032
BlsI GCNGC 5 cut(s) 34, 43, 67, 131, 1033
BmcAI AGTACT 1 cut(s) 142
Bme1390I CCNGG 2 cut(s) 515, 1040
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 2 cut(s) 515, 1040
BpmI CTGGAG 1 cut(s) 66
BpuEI CTTGAG 2 cut(s) 85, 121
BpuMI CCSGG 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 959
BsaHI GRCGYC 1 cut(s) 459
Bsc4I CCNNNNNNNGG 1 cut(s) 930
Bse1I ACTGG 2 cut(s) 452, 1032
Bse21I CCTNAGG 1 cut(s) 1016
Bse3DI GCAATG 2 cut(s) 213, 1168
Bse8I GATNNNNATC 1 cut(s) 959
BseBI CCWGG 1 cut(s) 1040
BseGI GGATG 7 cut(s) 10, 439, 652, 711, 1058, 1107, 1119
BseJI GATNNNNATC 1 cut(s) 959
BseLI CCNNNNNNNGG 1 cut(s) 930
BseMI GCAATG 2 cut(s) 213, 1168
BseMII CTCAG 3 cut(s) 158, 279, 441
BseNI ACTGG 2 cut(s) 452, 1032
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 4 cut(s) 44, 53, 77, 1043
BseYI CCCAGC 1 cut(s) 801
BsgI GTGCAG 1 cut(s) 108
BshFI GGCC 2 cut(s) 120, 734
BsiHKAI GWGCWC 2 cut(s) 491, 641
BsiSI CCGG 1 cut(s) 515
BslFI GGGAC 2 cut(s) 67, 497
BslI CCNNNNNNNGG 1 cut(s) 930
BsmAI GTCTC 2 cut(s) 81, 290
BsmBI CGTCTC 1 cut(s) 290
BsmFI GGGAC 2 cut(s) 67, 497
BsnI GGCC 2 cut(s) 120, 734
Bsp1286I GDGCHC 2 cut(s) 491, 641
Bsp1407I TGTACA 1 cut(s) 1080
Bsp143I GATC 5 cut(s) 7, 112, 257, 316, 717
BspACI CCGC 2 cut(s) 130, 417
BspANI GGCC 2 cut(s) 120, 734
BspCNI CTCAG 3 cut(s) 157, 278, 440
BspLI GGNNCC 1 cut(s) 318
BspMI ACCTGC 1 cut(s) 334
BspPI GGATC 3 cut(s) 311, 324, 725
BsrDI GCAATG 2 cut(s) 213, 1168
BsrGI TGTACA 1 cut(s) 1080
BsrI ACTGG 2 cut(s) 452, 1032
BssMI GATC 5 cut(s) 7, 112, 257, 316, 717
BssNI GRCGYC 1 cut(s) 459
Bst2UI CCWGG 1 cut(s) 1040
Bst4CI ACNGT 4 cut(s) 290, 643, 742, 844
Bst6I CTCTTC 1 cut(s) 542
BstACI GRCGYC 1 cut(s) 459
BstAUI TGTACA 1 cut(s) 1080
BstC8I GCNNGC 3 cut(s) 30, 127, 360
BstDEI CTNAG 4 cut(s) 144, 265, 427, 1016
BstEII GGTNACC 2 cut(s) 598, 1009
BstF5I GGATG 7 cut(s) 10, 439, 652, 711, 1058, 1107, 1119
BstKTI GATC 5 cut(s) 10, 115, 260, 319, 720
BstMAI GTCTC 2 cut(s) 81, 290
BstMBI GATC 5 cut(s) 7, 112, 257, 316, 717
BstMWI GCNNNNNNNGC 5 cut(s) 38, 41, 126, 785, 1167
BstNI CCWGG 1 cut(s) 1040
BstPI GGTNACC 2 cut(s) 598, 1009
BstSCI CCNGG 2 cut(s) 513, 1038
BstSFI CTRYAG 2 cut(s) 762, 796
BstSLI GKGCMC 1 cut(s) 491
BstV1I GCAGC 4 cut(s) 44, 53, 77, 1043
BstX2I RGATCY 1 cut(s) 316
BstYI RGATCY 1 cut(s) 316
Bsu36I CCTNAGG 1 cut(s) 1016
BsuRI GGCC 2 cut(s) 120, 734
BtsCI GGATG 7 cut(s) 10, 439, 652, 711, 1058, 1107, 1119
BtsIMutI CAGTG 3 cut(s) 282, 459, 840
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 3 cut(s) 30, 127, 360
Cfr13I GGNCC 1 cut(s) 119
CseI GACGC 1 cut(s) 467
Csp6I GTAC 4 cut(s) 141, 466, 943, 1081
CviAII CATG 2 cut(s) 463, 590
CviQI GTAC 4 cut(s) 141, 466, 943, 1081
DdeI CTNAG 4 cut(s) 144, 265, 427, 1016
DpnI GATC 5 cut(s) 9, 114, 259, 318, 719
DpnII GATC 5 cut(s) 7, 112, 257, 316, 717
DraIII CACNNNGTG 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 542
EarI CTCTTC 1 cut(s) 542
Eco57I CTGAAG 1 cut(s) 1109
Eco81I CCTNAGG 1 cut(s) 1016
Eco91I GGTNACC 2 cut(s) 598, 1009
EcoO65I GGTNACC 2 cut(s) 598, 1009
EcoRII CCWGG 1 cut(s) 1038
Esp3I CGTCTC 1 cut(s) 290
FaeI CATG 2 cut(s) 466, 593
FalI AAGNNNNNCTT 2 cut(s) 713, 745
FaqI GGGAC 2 cut(s) 67, 497
FatI CATG 2 cut(s) 462, 589
FbaI TGATCA 1 cut(s) 7
FblI GTMKAC 1 cut(s) 405
Fnu4HI GCNGC 5 cut(s) 33, 42, 66, 130, 1032
FokI GGATG 7 cut(s) 17, 446, 659, 718, 1065, 1106, 1114
Fsp4HI GCNGC 5 cut(s) 33, 42, 66, 130, 1032
FspBI CTAG 1 cut(s) 57
GluI GCNGC 5 cut(s) 33, 42, 66, 130, 1032
GsaI CCCAGC 1 cut(s) 805
GsuI CTGGAG 1 cut(s) 66
HaeIII GGCC 2 cut(s) 120, 734
HapII CCGG 1 cut(s) 515
HgaI GACGC 1 cut(s) 467
Hin1I GRCGYC 1 cut(s) 459
Hin1II CATG 2 cut(s) 466, 593
HincII GTYRAC 1 cut(s) 1186
HindII GTYRAC 1 cut(s) 1186
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 5 cut(s) 301, 308, 623, 960, 1145
HpaII CCGG 1 cut(s) 515
HphI GGTGA 5 cut(s) 176, 274, 515, 610, 1003
Hpy166II GTNNAC 5 cut(s) 282, 406, 457, 489, 1186
Hpy188I TCNGA 1 cut(s) 1069
Hpy188III TCNNGA 2 cut(s) 84, 305
Hpy8I GTNNAC 5 cut(s) 282, 406, 457, 489, 1186
Hpy99I CGWCG 2 cut(s) 258, 929
HpyAV CCTTC 6 cut(s) 44, 237, 373, 691, 1084, 1190
HpyCH4III ACNGT 4 cut(s) 290, 643, 742, 844
HpyCH4IV ACGT 1 cut(s) 253
HpyCH4V TGCA 7 cut(s) 32, 125, 206, 489, 788, 893, 1134
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 41, 126, 785, 1167
HpyF3I CTNAG 4 cut(s) 144, 265, 427, 1016
HpySE526I ACGT 1 cut(s) 253
Hsp92I GRCGYC 1 cut(s) 459
Hsp92II CATG 2 cut(s) 466, 593
Ksp22I TGATCA 1 cut(s) 7
Kzo9I GATC 5 cut(s) 7, 112, 257, 316, 717
LmnI GCTCC 1 cut(s) 274
Lsp1109I GCAGC 4 cut(s) 44, 53, 77, 1043
MaeI CTAG 1 cut(s) 57
MaeII ACGT 1 cut(s) 253
MaeIII GTNAC 8 cut(s) 439, 478, 503, 598, 611, 826, 835, 1009
MalI GATC 5 cut(s) 9, 114, 259, 318, 719
MboI GATC 5 cut(s) 7, 112, 257, 316, 717
MboII GAAGA 6 cut(s) 186, 189, 529, 541, 894, 989
MflI RGATCY 1 cut(s) 316
MhlI GDGCHC 2 cut(s) 491, 641
MluCI AATT 3 cut(s) 190, 390, 1139
MnlI CCTC 6 cut(s) 31, 143, 696, 784, 872, 1000
MroXI GAANNNNTTC 1 cut(s) 656
MseI TTAA 1 cut(s) 737
MslI CAYNNNNRTG 1 cut(s) 1161
MspA1I CMGCKG 3 cut(s) 44, 417, 801
MspI CCGG 1 cut(s) 515
MspR9I CCNGG 2 cut(s) 515, 1040
MvaI CCWGG 1 cut(s) 1040
MwoI GCNNNNNNNGC 5 cut(s) 38, 41, 126, 785, 1167
NciI CCSGG 1 cut(s) 515
NdeII GATC 5 cut(s) 7, 112, 257, 316, 717
NlaIII CATG 2 cut(s) 466, 593
NlaIV GGNNCC 1 cut(s) 318
NmuCI GTSAC 7 cut(s) 439, 478, 503, 598, 611, 835, 1009
PdmI GAANNNNTTC 1 cut(s) 656
PfeI GAWTC 5 cut(s) 301, 308, 623, 960, 1145
PflFI GACNNNGTC 1 cut(s) 509
PfoI TCCNGGA 1 cut(s) 513
PkrI GCNGC 5 cut(s) 34, 43, 67, 131, 1033
Psp6I CCWGG 1 cut(s) 1038
PspEI GGTNACC 2 cut(s) 598, 1009
PspFI CCCAGC 1 cut(s) 801
PspGI CCWGG 1 cut(s) 1038
PspN4I GGNNCC 1 cut(s) 318
PspPI GGNCC 1 cut(s) 119
PsuI RGATCY 1 cut(s) 316
PsyI GACNNNGTC 1 cut(s) 509
PvuII CAGCTG 2 cut(s) 44, 801
RsaI GTAC 4 cut(s) 142, 467, 944, 1082
RsaNI GTAC 4 cut(s) 141, 466, 943, 1081
RseI CAYNNNNRTG 1 cut(s) 1161
SaqAI TTAA 1 cut(s) 737
SatI GCNGC 5 cut(s) 33, 42, 66, 130, 1032
Sau3AI GATC 5 cut(s) 7, 112, 257, 316, 717
Sau96I GGNCC 1 cut(s) 119
ScaI AGTACT 1 cut(s) 142
ScrFI CCNGG 2 cut(s) 515, 1040
SduI GDGCHC 2 cut(s) 491, 641
SfcI CTRYAG 2 cut(s) 762, 796
SmiMI CAYNNNNRTG 1 cut(s) 1161
SmlI CTYRAG 2 cut(s) 100, 136
SmoI CTYRAG 2 cut(s) 100, 136
Sse9I AATT 3 cut(s) 190, 390, 1139
SsiI CCGC 2 cut(s) 130, 417
SspMI CTAG 1 cut(s) 57
StyD4I CCNGG 2 cut(s) 513, 1038
TaaI ACNGT 4 cut(s) 290, 643, 742, 844
TaiI ACGT 1 cut(s) 256
TaqI TCGA 2 cut(s) 256, 963
TasI AATT 3 cut(s) 190, 390, 1139
TatI WGTACW 3 cut(s) 140, 465, 1080
TauI GCSGC 1 cut(s) 132
TfiI GAWTC 5 cut(s) 301, 308, 623, 960, 1145
Tru1I TTAA 1 cut(s) 737
Tru9I TTAA 1 cut(s) 737
TscAI CASTG 3 cut(s) 282, 459, 840
TseFI GTSAC 7 cut(s) 439, 478, 503, 598, 611, 835, 1009
TseI GCWGC 4 cut(s) 32, 41, 65, 1031
Tsp45I GTSAC 7 cut(s) 439, 478, 503, 598, 611, 835, 1009
TspDTI ATGAA 3 cut(s) 355, 541, 562
TspRI CASTG 3 cut(s) 282, 459, 840
Tth111I GACNNNGTC 1 cut(s) 509
VneI GTGCAC 1 cut(s) 487
XapI RAATTY 1 cut(s) 190
XmiI GTMKAC 1 cut(s) 405
XmnI GAANNNNTTC 1 cut(s) 656
XspI CTAG 1 cut(s) 57
ZrmI AGTACT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.