Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0008589

Physical Location & Seq

Genomic Coordinates Forward (+)

32186603 .. 32189499

Gene Structure 2897 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g40750.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

ATGAATAAACTCCCATTACGTCAAATTTCAATCTTGCCCACTTCAATTCCTAAAGAACCCACCTTTACAAAACCCTCAGTTTATGTGAAACTGGAAACAAGGAATTGGAACCCAATTCCTATTCCCCACAGAACCATTCCTGAACCCAAAGGCCAAGACCTTGATTTTGTTAATGTTGTAAACAGCCATTTGATTCACTCTGACTGGGCCAAGCTCAACTCTTTAGCCAATGGTCTAACTGCTTTCAGAGTGAAACATGTTTTCCTGAAAGTTCAGAAAGACTATGTTGTTTCCCTTGAGTTTTTCAACTGGGTTGGAACTCACAAACCGACTTCACTTACCCTCGAAACACATTCCATGATTCTCCACATTCTCACCAAGTATCGAAAGTTCAAGTCTGCCGAGTCGATTTTGAGGAAGATTCTTGTGCCAGGGAACATAGACTTGCCTTCCAAGCTGTTTGAGGCGATACTGTATTCGTACCGCTCGTGTGATTCCTCTCCTCGGGTTTTTGACTCGCTTTTCAAGACATTTGCGCATATGAAGAAGTTTAGGAATGCCACAGACACGTTTTGTAGGATGAAGGATTATGGGTTTTACCCGACGGTTGAGTCTTGCAATGCATATGTGAGCTCATTGCTTGATTTGAATAGAGCTGATGTTGCTTTGGCGTTCTATAGAGAAATGAGGCGGTGTCGGATTTCACCAAATGTTTATACTCTTAATATGGTTATGTGTGCTTACTGTAGAGTGGGGAAATTAGAGAATGCTGTTGAGGTCCTTGAGAAGATGGAGAGCATGGGTTTCAGTGCCACCGTTGTGTCGTATAATACACTGATTGCAGGACATTGCAATAAGGGTCTTCTGAGTTCTGCTGTGAAGTTCAAAAACTTGATGGTGAAGAATGGATTGCACCCGAGTGTGGTAACTTTTAACACGCTTATTGATGGGTTCTGTAAGCAAGGTAAACTACCAGAAGCAAATATAATTTTTAGTGAGATGAAAGCTGCAAATGTGGCTCCTAATACAGTAACCTATAATACCCTGATAAATGGGTATGGTCAAGCAGGTAATAGTGAGATGGGTAGTAGGCTTTTTGAGGAGATGTCGAAGAATCGAGTAAGTGCTGATATTCTGACTTATAATGGACTGATTTTGGGACTATGCAAGGAGGGTAAAACGAAGAAAGCTGCATATCTGGTAAAAGAGCTTGACAGTAAGAACTTTGTCCCAAATGCCTCAACCTTTTCTGCCCTTGTTGGTGGGGAGTGTGTGAGAAAGAACTCAGATCGTGCTTTTCTGTTGTATAAAAGCATGGTCAGGAGCGGCTATCATCCTGATGAACATACTTTTAAGATGTTGTTGTCTAGTTTTTGCAATAATAGGGATTTTGATGGAGCAGTGGAAATCTTGAAAGAAATGTTTGAGAGAAATTTTGTTCCTGATTCAGATATCGTATCTGATCTTTGTCTGGGACTTAGACGTTGCGGGAAAGGAAAATTGGTAGAATTGCTATGCCGAGAGATGGAATCTAGACGCCTCATACCTCAAGATTTTGACATGGCTAAGATTATCTGCTGTGGAGAAGAGAATGAGAATGCAGAAGTAGGAAACATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0000963 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0090615 GO:0090617 GO:0097159 GO:0140053 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

540

Amino Acids

60.93

Weight (kDa)

9.03

Isoelectric Point (pI)

34.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	182 - 280	6.8e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	201 - 245	1.8e-06	PPR repeat family
PPR_3	PF13812	227 - 280	9.5e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	234 - 265	9.8e-09	PPR repeat
PPR_2	PF13041	236 - 274	2.4e-07	PPR repeat family
PPR	PF01535	240 - 269	7.7e-06	PPR repeat
PPR_1	PF12854	267 - 300	1e-07	PPR repeat
PPR_2	PF13041	272 - 320	6.9e-17	PPR repeat family
PPR_3	PF13812	299 - 355	3.2e-13	Pentatricopeptide repeat domain
PPR_long	PF17177	301 - 419	1e-05	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	302 - 335	9e-12	PPR repeat
PPR_2	PF13041	306 - 353	2.1e-18	PPR repeat family
PPR	PF01535	309 - 338	1.6e-07	PPR repeat
PPR_1	PF12854	338 - 370	7.1e-09	PPR repeat
PPR_2	PF13041	341 - 390	8.2e-16	PPR repeat family
PPR	PF01535	344 - 373	3.9e-08	PPR repeat
PPR_long	PF17177	365 - 482	1.8e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	374 - 403	6.1e-06	PPR repeat
PPR_2	PF13041	377 - 424	2.9e-09	PPR repeat family
PPR_2	PF13041	419 - 460	5.6e-07	PPR repeat family
PPR_1	PF12854	443 - 474	3.1e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0008589)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26680 AT4G26680
fragaria_vesca	FvH4_6g40750
malus_domestica	MD17G1115600.v1.1
prunus_persica	Prupe.3G199000_v2.0.a1 Prupe.3G199000_v2.0.a1
pyrus_communis	pycom17g10680
rosa_chinensis	RchiOBHm_Chr2g0156201 RchiOBHm_Chr4g0385191
rosa_laevigata	RLG00000010250 RLG00000020895
rosa_multiflora	Rmu_sc0000260.1_g000011 Rmu_sc0003086.1_g000003
rosa_roxburghii	Rroxscaffold_2G00093150 Rroxscaffold_5G00333090
rosa_rugosa	Rorug02G0452500
rosa_samantha	Rh2AG519000 Rh2BG530400 Rh2CG504000 Rh2DG539400 Rh4CG003600
rosa_wichuraiana	Rw2G042750 Rw4G000590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1143
AasI	GACNNNNNNGTC	1 cut(s)	610
Acc16I	TGCGCA	1 cut(s)	537
Acc36I	ACCTGC	1 cut(s)	1058
AccBSI	CCGCTC	2 cut(s)	486, 1326
AciI	CCGC	4 cut(s)	484, 691, 1326, 1488
AcsI	RAATTY	2 cut(s)	24, 1432
AcyI	GRCGYC	1 cut(s)	1537
AfaI	GTAC	1 cut(s)	482
AfiI	CCNNNNNNNGG	3 cut(s)	504, 922, 1525
AflIII	ACRYGT	3 cut(s)	256, 567, 1614
AgsI	TTSAA	8 cut(s)	30, 45, 307, 394, 526, 649, 886, 1414
AjnI	CCWGG	1 cut(s)	430
AjuI	GAANNNNNNNTTGG	2 cut(s)	1484, 1516
AleI	CACNNNNGTG	2 cut(s)	818, 918
AluBI	AGCT	7 cut(s)	214, 457, 633, 656, 1007, 1190, 1210
AluI	AGCT	7 cut(s)	214, 457, 633, 656, 1007, 1190, 1210
Alw21I	GWGCWC	1 cut(s)	635
Ama87I	CYCGRG	2 cut(s)	504, 916
AoxI	GGCC	2 cut(s)	151, 207
ApeKI	GCWGC	2 cut(s)	1007, 1190
ApoI	RAATTY	2 cut(s)	24, 1432
ArsI	GACNNNNNNTTYG	2 cut(s)	149, 181
AspLEI	GCGC	1 cut(s)	538
AspS9I	GGNCC	2 cut(s)	207, 778
AsuHPI	GGTGA	3 cut(s)	367, 696, 910
AvaI	CYCGRG	2 cut(s)	504, 916
AvaII	GGWCC	1 cut(s)	778
BanII	GRGCYC	1 cut(s)	635
BauI	CACGAG	1 cut(s)	487
BbsI	GAAGAC	1 cut(s)	854
Bbv12I	GWGCWC	1 cut(s)	635
BbvI	GCAGC	2 cut(s)	994, 1177
BccI	CCATC	6 cut(s)	784, 889, 941, 1075, 1388, 1519
BciT130I	CCWGG	1 cut(s)	432
BfaI	CTAG	2 cut(s)	1368, 1533
BfmI	CTRYAG	2 cut(s)	676, 745
BfuAI	ACCTGC	1 cut(s)	1058
BisI	GCNGC	3 cut(s)	1008, 1191, 1327
BlsI	GCNGC	3 cut(s)	1009, 1192, 1328
Bme1390I	CCNGG	1 cut(s)	432
Bme18I	GGWCC	1 cut(s)	778
BmeT110I	CYCGRG	2 cut(s)	504, 916
BmgT120I	GGNCC	2 cut(s)	207, 778
BmiI	GGNNCC	2 cut(s)	110, 1020
BmrFI	CCNGG	1 cut(s)	432
BmrI	ACTGGG	2 cut(s)	214, 319
BmuI	ACTGGG	2 cut(s)	214, 319
BpiI	GAAGAC	1 cut(s)	854
BpuEI	CTTGAG	3 cut(s)	317, 803, 1533
BsaHI	GRCGYC	1 cut(s)	1537
BsaJI	CCNNGG	2 cut(s)	431, 503
Bsc4I	CCNNNNNNNGG	3 cut(s)	504, 922, 1525
Bse1I	ACTGG	3 cut(s)	96, 209, 314
Bse3DI	GCAATG	3 cut(s)	625, 635, 847
BseBI	CCWGG	1 cut(s)	432
BseDI	CCNNGG	2 cut(s)	431, 503
BseGI	GGATG	2 cut(s)	585, 1333
BseLI	CCNNNNNNNGG	3 cut(s)	504, 922, 1525
BseMI	GCAATG	3 cut(s)	625, 635, 847
BseMII	CTCAG	3 cut(s)	90, 857, 1299
BseNI	ACTGG	3 cut(s)	96, 209, 314
BseRI	GAGGAG	2 cut(s)	492, 1115
BseXI	GCAGC	2 cut(s)	994, 1177
BshFI	GGCC	2 cut(s)	153, 209
BsiHKAI	GWGCWC	1 cut(s)	635
BsiHKCI	CYCGRG	2 cut(s)	504, 916
BslFI	GGGAC	3 cut(s)	1173, 1214, 1488
BslI	CCNNNNNNNGG	3 cut(s)	504, 922, 1525
BsmFI	GGGAC	3 cut(s)	1173, 1214, 1488
BsmI	GAATGC	3 cut(s)	562, 772, 1603
BsnI	GGCC	2 cut(s)	153, 209
BsoBI	CYCGRG	2 cut(s)	504, 916
Bsp1286I	GDGCHC	1 cut(s)	635
Bsp143I	GATC	2 cut(s)	1288, 1462
BspACI	CCGC	4 cut(s)	484, 691, 1326, 1488
BspANI	GGCC	2 cut(s)	153, 209
BspCNI	CTCAG	3 cut(s)	89, 858, 1298
BspLI	GGNNCC	2 cut(s)	110, 1020
BspMI	ACCTGC	1 cut(s)	1058
BsrBI	CCGCTC	2 cut(s)	486, 1326
BsrDI	GCAATG	3 cut(s)	625, 635, 847
BsrI	ACTGG	3 cut(s)	96, 209, 314
BssECI	CCNNGG	2 cut(s)	431, 503
BssMI	GATC	2 cut(s)	1288, 1462
BssNI	GRCGYC	1 cut(s)	1537
BssSI	CACGAG	1 cut(s)	487
Bst2BI	CACGAG	1 cut(s)	487
Bst2UI	CCWGG	1 cut(s)	432
Bst4CI	ACNGT	6 cut(s)	474, 607, 746, 817, 1030, 1217
Bst6I	CTCTTC	1 cut(s)	1581
BstACI	GRCGYC	1 cut(s)	1537
BstDEI	CTNAG	5 cut(s)	76, 866, 1285, 1478, 1566
BstF5I	GGATG	2 cut(s)	585, 1333
BstHHI	GCGC	1 cut(s)	538
BstKTI	GATC	2 cut(s)	1291, 1465
BstMBI	GATC	2 cut(s)	1288, 1462
BstMWI	GCNNNNNNNGC	3 cut(s)	454, 662, 1016
BstNI	CCWGG	1 cut(s)	432
BstNSI	RCATGY	2 cut(s)	260, 1618
BstSCI	CCNGG	1 cut(s)	430
BstSFI	CTRYAG	2 cut(s)	676, 745
BstV1I	GCAGC	2 cut(s)	994, 1177
BstV2I	GAAGAC	1 cut(s)	854
BsuRI	GGCC	2 cut(s)	153, 209
BtsCI	GGATG	2 cut(s)	585, 1333
BtsI	GCAGTG	1 cut(s)	1407
BtsIMutI	CAGTG	3 cut(s)	814, 833, 1407
BveI	ACCTGC	1 cut(s)	1058
CfoI	GCGC	1 cut(s)	538
Cfr13I	GGNCC	2 cut(s)	207, 778
CseI	GACGC	1 cut(s)	1545
Csp6I	GTAC	1 cut(s)	481
CviAII	CATG	6 cut(s)	257, 358, 799, 1315, 1561, 1615
CviQI	GTAC	1 cut(s)	481
DdeI	CTNAG	5 cut(s)	76, 866, 1285, 1478, 1566
DpnI	GATC	2 cut(s)	1290, 1464
DpnII	GATC	2 cut(s)	1288, 1462
DrdI	GACNNNNNNGTC	1 cut(s)	610
DseDI	GACNNNNNNGTC	1 cut(s)	610
Eam1104I	CTCTTC	1 cut(s)	1581
EarI	CTCTTC	1 cut(s)	1581
Ecl136II	GAGCTC	1 cut(s)	633
Eco24I	GRGCYC	1 cut(s)	635
Eco32I	GATATC	1 cut(s)	1453
Eco47I	GGWCC	1 cut(s)	778
Eco53kI	GAGCTC	1 cut(s)	633
Eco88I	CYCGRG	2 cut(s)	504, 916
EcoICRI	GAGCTC	1 cut(s)	633
EcoO109I	RGGNCCY	1 cut(s)	778
EcoRII	CCWGG	1 cut(s)	430
EcoRV	GATATC	1 cut(s)	1453
EcoT22I	ATGCAT	1 cut(s)	625
EcoT38I	GRGCYC	1 cut(s)	635
FaeI	CATG	6 cut(s)	260, 361, 802, 1318, 1564, 1618
FaqI	GGGAC	3 cut(s)	1173, 1214, 1488
FatI	CATG	6 cut(s)	256, 357, 798, 1314, 1560, 1614
FauI	CCCGC	1 cut(s)	1481
FauNDI	CATATG	2 cut(s)	540, 625
Fnu4HI	GCNGC	3 cut(s)	1008, 1191, 1327
FokI	GGATG	2 cut(s)	592, 1320
FriOI	GRGCYC	1 cut(s)	635
Fsp4HI	GCNGC	3 cut(s)	1008, 1191, 1327
FspBI	CTAG	2 cut(s)	1368, 1533
FspI	TGCGCA	1 cut(s)	537
GlaI	GCGC	1 cut(s)	537
GluI	GCNGC	3 cut(s)	1008, 1191, 1327
HaeIII	GGCC	2 cut(s)	153, 209
HgaI	GACGC	1 cut(s)	1545
HhaI	GCGC	1 cut(s)	538
Hin1I	GRCGYC	1 cut(s)	1537
Hin1II	CATG	6 cut(s)	260, 361, 802, 1318, 1564, 1618
Hin6I	GCGC	1 cut(s)	536
HinP1I	GCGC	1 cut(s)	536
HphI	GGTGA	3 cut(s)	367, 696, 910
Hpy166II	GTNNAC	2 cut(s)	181, 968
Hpy188I	TCNGA	9 cut(s)	202, 248, 276, 699, 867, 1137, 1288, 1450, 1462
Hpy188III	TCNNGA	9 cut(s)	140, 265, 526, 1321, 1337, 1411, 1442, 1533, 1550
Hpy8I	GTNNAC	2 cut(s)	181, 968
Hpy99I	CGWCG	1 cut(s)	607
HpyAV	CCTTC	2 cut(s)	459, 577
HpyCH4III	ACNGT	6 cut(s)	474, 607, 746, 817, 1030, 1217
HpyCH4IV	ACGT	3 cut(s)	19, 569, 1483
HpyF10VI	GCNNNNNNNGC	3 cut(s)	454, 662, 1016
HpyF3I	CTNAG	5 cut(s)	76, 866, 1285, 1478, 1566
HpySE526I	ACGT	3 cut(s)	19, 569, 1483
Hsp92I	GRCGYC	1 cut(s)	1537
Hsp92II	CATG	6 cut(s)	260, 361, 802, 1318, 1564, 1618
HspAI	GCGC	1 cut(s)	536
Kzo9I	GATC	2 cut(s)	1288, 1462
LmnI	GCTCC	3 cut(s)	1024, 1323, 1397
Lsp1109I	GCAGC	2 cut(s)	994, 1177
MaeI	CTAG	2 cut(s)	1368, 1533
MaeII	ACGT	3 cut(s)	19, 569, 1483
MaeIII	GTNAC	2 cut(s)	925, 1030
MalI	GATC	2 cut(s)	1290, 1464
MbiI	CCGCTC	2 cut(s)	486, 1326
MboI	GATC	2 cut(s)	1288, 1462
MboII	GAAGA	8 cut(s)	430, 556, 799, 854, 913, 1123, 1195, 1598
MhlI	GDGCHC	1 cut(s)	635
MluCI	AATT	9 cut(s)	24, 45, 103, 114, 758, 987, 1432, 1499, 1508
MlyI	GAGTC	3 cut(s)	413, 509, 620
MmeI	TCCRAC	2 cut(s)	295, 677
Mph1103I	ATGCAT	1 cut(s)	625
MseI	TTAA	4 cut(s)	171, 723, 933, 1353
MslI	CAYNNNNRTG	3 cut(s)	818, 918, 1338
MspR9I	CCNGG	1 cut(s)	432
Mva1269I	GAATGC	3 cut(s)	562, 772, 1603
MvaI	CCWGG	1 cut(s)	432
MwoI	GCNNNNNNNGC	3 cut(s)	454, 662, 1016
NdeI	CATATG	2 cut(s)	540, 625
NdeII	GATC	2 cut(s)	1288, 1462
NlaIII	CATG	6 cut(s)	260, 361, 802, 1318, 1564, 1618
NlaIV	GGNNCC	2 cut(s)	110, 1020
NmeAIII	GCCGAG	2 cut(s)	427, 1544
NsbI	TGCGCA	1 cut(s)	537
NsiI	ATGCAT	1 cut(s)	625
NspI	RCATGY	2 cut(s)	260, 1618
OliI	CACNNNNGTG	2 cut(s)	818, 918
PciI	ACATGT	2 cut(s)	256, 1614
PcsI	WCGNNNNNNNCGW	1 cut(s)	485
PctI	GAATGC	3 cut(s)	562, 772, 1603
PfeI	GAWTC	7 cut(s)	193, 361, 421, 494, 1114, 1445, 1529
PkrI	GCNGC	3 cut(s)	1009, 1192, 1328
PleI	GAGTC	3 cut(s)	412, 509, 619
PpsI	GAGTC	3 cut(s)	412, 509, 619
PpuMI	RGGWCCY	1 cut(s)	778
PscI	ACATGT	2 cut(s)	256, 1614
PsiI	TTATAA	1 cut(s)	1143
Psp124BI	GAGCTC	1 cut(s)	635
Psp5II	RGGWCCY	1 cut(s)	778
Psp6I	CCWGG	1 cut(s)	430
PspGI	CCWGG	1 cut(s)	430
PspN4I	GGNNCC	2 cut(s)	110, 1020
PspPI	GGNCC	2 cut(s)	207, 778
PspPPI	RGGWCCY	1 cut(s)	778
PsrI	GAACNNNNNNTAC	2 cut(s)	374, 406
RsaI	GTAC	1 cut(s)	482
RsaNI	GTAC	1 cut(s)	481
RseI	CAYNNNNRTG	3 cut(s)	818, 918, 1338
SacI	GAGCTC	1 cut(s)	635
SaqAI	TTAA	4 cut(s)	171, 723, 933, 1353
SatI	GCNGC	3 cut(s)	1008, 1191, 1327
Sau3AI	GATC	2 cut(s)	1288, 1462
Sau96I	GGNCC	2 cut(s)	207, 778
SchI	GAGTC	3 cut(s)	413, 509, 620
ScrFI	CCNGG	1 cut(s)	432
SduI	GDGCHC	1 cut(s)	635
SfcI	CTRYAG	2 cut(s)	676, 745
SinI	GGWCC	1 cut(s)	778
SmiMI	CAYNNNNRTG	3 cut(s)	818, 918, 1338
SmlI	CTYRAG	3 cut(s)	296, 782, 1548
SmoI	CTYRAG	3 cut(s)	296, 782, 1548
Sse9I	AATT	9 cut(s)	24, 45, 103, 114, 758, 987, 1432, 1499, 1508
SsiI	CCGC	4 cut(s)	484, 691, 1326, 1488
SspMI	CTAG	2 cut(s)	1368, 1533
SstI	GAGCTC	1 cut(s)	635
StyD4I	CCNGG	1 cut(s)	430
TaaI	ACNGT	6 cut(s)	474, 607, 746, 817, 1030, 1217
TaiI	ACGT	3 cut(s)	22, 572, 1486
TaqI	TCGA	5 cut(s)	345, 385, 407, 1109, 1117
TasI	AATT	9 cut(s)	24, 45, 103, 114, 758, 987, 1432, 1499, 1508
TauI	GCSGC	1 cut(s)	1329
TfiI	GAWTC	7 cut(s)	193, 361, 421, 494, 1114, 1445, 1529
Tru1I	TTAA	4 cut(s)	171, 723, 933, 1353
Tru9I	TTAA	4 cut(s)	171, 723, 933, 1353
TscAI	CASTG	3 cut(s)	814, 840, 1407
TseI	GCWGC	2 cut(s)	1007, 1190
TspDTI	ATGAA	5 cut(s)	17, 557, 596, 1016, 1356
TspRI	CASTG	3 cut(s)	814, 840, 1407
VpaK11BI	GGWCC	1 cut(s)	778
XapI	RAATTY	2 cut(s)	24, 1432
XbaI	TCTAGA	1 cut(s)	1532
XceI	RCATGY	2 cut(s)	260, 1618
XspI	CTAG	2 cut(s)	1368, 1533
Zsp2I	ATGCAT	1 cut(s)	625

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.