MD17G1115600.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0008589
Physical Location & Seq
Forward (+)
9887473 .. 9888990
1518 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1115600.v1.1.491

Sequence Viewer

Length: 1518 bp
ATGAATCGGAATCCGATTCCTATACCCCACAGAACGATTCCTGAACCCAAAGGGCAGGACCTTGATTTTGTCAATGTTGTAAACAGCCATCTGATTCACTCGGATTGGGCTAAGCTTAATCCCTTATCGACCGGTCTAACTGCTTTTAGAGTTAAACATATATTGCTGAAAATTCAGAAGGATTATGTTCTTTCCCTTGAGTTTTTCAATTGGGTTGCTGCTCGAAACCCGAATGCACATACCCTCGAAACACATTCCATGATTCTCCACATTCTCACCAAGTACCGGAAGTTCAAATCCGCGGAGTCTATTTCGAAGAAGATTCTTGTTTCGGGCTCAATTGATTTGCCTTCCAAGTTGTTTGAGGCGATTCTGTATTCGTACCGGTTGTGTGACTCATCTCCTCGTGTCTTTGATTCGCTGTTCAAGACTTTCGCGCATTTGAAGAAGTTTAGGAATGCCACAAACATGTTTTGTCAGATGAAGGAGTACGGTTTCTTCCCAACGGTTGAGTCTTGCAATGCGTATTTGAGCTCATTGCTTGATTTGCATAGAGCGGACGTTGCTTTGGCGTTCTATAGAGAAATGCGGCGTTGTAGGATTTCGCCCAATGTCTATACTCTTAATATGGTTATGGCTGCTTACTGCAAATCGGGAAAATTAGAGAGCGCTGTTGAGGTGCTCGAGGAGATGGAGAGCATGGGTTGTAGTCCTAGTGTGGTGTCGTATAATACACTGATTGCGGGACACTGTGATAAGGGTCTTATGAGTTCGGCTTTGAAGTTCAAAAACTTGATGGCGAAGAATGGATTGCACCCTACTGTGGTAACTTTTAACACGCTTATTGACGGCTTTTGCAAGGCAGGAAAACTACAAGAAGCAAATAGAGTTTTTAGCGAGATGAAGGCCGCAAATGTGGCTACTAATACAGTAACTTACAATAGTTTGATAAACGTGTACAGCCAGGCAGGTAATAGTGAGATGGGCACTAGGCTTTTCGAGGAGATGTCAAAGAACCGAGTAAATGCTGATATACTGACTTATAATGCACTGATATTGGGACTCTGCAAGGAGGGTAAAACAAAGAAAGCTGCGTATCTGGTTAAAGAACTTGATGCAAAGCACTTTGTCCCGAATGCCTCAACCTTTTCTGCCCTTATTGTAGGACAGTGTGTCCGAAAGAATGCTGATCGTGCCTTTCAGTTATGCCAAAGCATGATAAGAAGTGGTCATCATCCTGATGAGCAAACTTTGAAGACGCTTTTATCAAGCTTCTGCGATAACAGAGATTTTGATGGAGCAGTCCAAGTCTTGGAAGAAATGGTAGAGAGATCTATTGCTCTTGATTCAGGTATCTTATCTGATTTATGTCTAGGACTTCACCGATGTGGGAAAGAAGAATTGGTAAAGTCATTATGCGGTAAGTTGGAAGCTAGACGACTCATGCCTCAAGGTTTTGATAAAAGGTCGTACCCAGTGCACAAGGTTCCCGCTTTACGCAGGGTCTGGGAGAGGTGA

Protein Analysis

506

Amino Acids

56.92

Weight (kDa)

9.18

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 148 - 248 5.2e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 168 - 211 3.3e-06 PPR repeat family
PPR_3 PF13812 194 - 247 1.4e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 201 - 232 3.7e-09 PPR repeat
PPR_2 PF13041 203 - 247 3e-14 PPR repeat family
PPR PF01535 207 - 236 2.5e-07 PPR repeat
PPR_1 PF12854 234 - 266 4.5e-09 PPR repeat
PPR_2 PF13041 238 - 287 2.1e-17 PPR repeat family
PPR_3 PF13812 266 - 320 2.8e-12 Pentatricopeptide repeat domain
PPR_long PF17177 269 - 386 1.1e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 269 - 302 1.4e-15 PPR repeat
PPR_2 PF13041 273 - 318 1e-16 PPR repeat family
PPR PF01535 276 - 305 3.2e-09 PPR repeat
TPR_24 PF23276 277 - 367 1.4e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 309 - 357 3.7e-14 PPR repeat family
PPR PF01535 311 - 340 2.2e-07 PPR repeat
PPR_3 PF13812 331 - 387 2.3e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 341 - 370 1.9e-06 PPR repeat
PPR_2 PF13041 344 - 391 1.8e-09 PPR repeat family
PPR_long PF17177 369 - 465 1.6e-06 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 378 - 427 1.2e-07 PPR repeat family
PPR_1 PF12854 410 - 442 2.3e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1044
Acc36I ACCTGC 1 cut(s) 959
AccB7I CCANNNNNTGG 1 cut(s) 1312
AccBSI CCGCTC 1 cut(s) 557
AccII CGCG 2 cut(s) 302, 437
AciI CCGC 8 cut(s) 300, 302, 557, 589, 743, 909, 1419, 1491
AcsI RAATTY 1 cut(s) 171
AfaI GTAC 5 cut(s) 284, 383, 491, 959, 1472
AfeI AGCGCT 1 cut(s) 670
AfiI CCNNNNNNNGG 3 cut(s) 285, 823, 1312
AflIII ACRYGT 2 cut(s) 468, 954
AgeI ACCGGT 2 cut(s) 131, 384
AgsI TTSAA 7 cut(s) 208, 295, 427, 445, 781, 787, 1255
AhdI GACNNNNNGTC 1 cut(s) 1172
AjnI CCWGG 1 cut(s) 963
AjuI GAANNNNNNNTTGG 2 cut(s) 1385, 1417
AleI CACNNNNGTG 1 cut(s) 1386
AluBI AGCT 5 cut(s) 115, 534, 1091, 1272, 1433
AluI AGCT 5 cut(s) 115, 534, 1091, 1272, 1433
Alw21I GWGCWC 3 cut(s) 536, 684, 1482
Alw44I GTGCAC 1 cut(s) 1478
AlwNI CAGNNNCTG 1 cut(s) 1506
Ama87I CYCGRG 1 cut(s) 683
Aor51HI AGCGCT 1 cut(s) 670
AoxI GGCC 1 cut(s) 906
ApaLI GTGCAC 1 cut(s) 1478
ApeKI GCWGC 3 cut(s) 218, 638, 1091
ApoI RAATTY 1 cut(s) 171
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
AsiGI ACCGGT 2 cut(s) 131, 384
AspLEI GCGC 2 cut(s) 439, 671
AspS9I GGNCC 1 cut(s) 58
AsuHPI GGTGA 2 cut(s) 268, 1373
AsuII TTCGAA 1 cut(s) 314
AvaI CYCGRG 1 cut(s) 683
AvaII GGWCC 1 cut(s) 58
BaeGI GKGCMC 2 cut(s) 989, 1482
BanII GRGCYC 2 cut(s) 338, 536
BarI GAAGNNNNNNTAC 2 cut(s) 482, 514
BauI CACGAG 1 cut(s) 405
BbsI GAAGAC 1 cut(s) 1262
Bbv12I GWGCWC 3 cut(s) 536, 684, 1482
BbvI GCAGC 3 cut(s) 205, 625, 1078
BccI CCATC 5 cut(s) 96, 685, 790, 976, 1289
BceAI ACGGC 1 cut(s) 865
BciT130I CCWGG 1 cut(s) 965
BfaI CTAG 4 cut(s) 714, 990, 1373, 1434
BfmI CTRYAG 1 cut(s) 577
BfoI RGCGCY 1 cut(s) 672
BfuAI ACCTGC 1 cut(s) 959
BglII AGATCT 1 cut(s) 1331
BisI GCNGC 5 cut(s) 219, 590, 639, 909, 1092
BlpI GCTNAGC 1 cut(s) 111
BlsI GCNGC 5 cut(s) 220, 591, 640, 910, 1093
Bme1390I CCNGG 1 cut(s) 965
Bme18I GGWCC 1 cut(s) 58
BmeRI GACNNNNNGTC 1 cut(s) 1172
BmeT110I CYCGRG 1 cut(s) 683
BmgT120I GGNCC 1 cut(s) 58
BmiI GGNNCC 1 cut(s) 1488
BmrFI CCNGG 1 cut(s) 965
BmrI ACTGGG 1 cut(s) 1469
BmsI GCATC 1 cut(s) 1105
BmuI ACTGGG 1 cut(s) 1469
BpiI GAAGAC 1 cut(s) 1262
Bpu1102I GCTNAGC 1 cut(s) 111
Bpu14I TTCGAA 1 cut(s) 314
BpuEI CTTGAG 2 cut(s) 218, 1434
BsaJI CCNNGG 1 cut(s) 300
BsaWI WCCGGW 3 cut(s) 131, 285, 384
Bsc4I CCNNNNNNNGG 3 cut(s) 285, 823, 1312
Bse118I RCCGGY 2 cut(s) 131, 384
Bse1I ACTGG 1 cut(s) 1475
Bse3DI GCAATG 2 cut(s) 526, 536
BseBI CCWGG 1 cut(s) 965
BseDI CCNNGG 1 cut(s) 300
BseGI GGATG 1 cut(s) 1234
BseLI CCNNNNNNNGG 3 cut(s) 285, 823, 1312
BseMI GCAATG 2 cut(s) 526, 536
BseNI ACTGG 1 cut(s) 1475
BseRI GAGGAG 3 cut(s) 393, 701, 1016
BseSI GKGCMC 2 cut(s) 989, 1482
BseXI GCAGC 3 cut(s) 205, 625, 1078
Bsh1236I CGCG 2 cut(s) 302, 437
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 1 cut(s) 908
BshTI ACCGGT 2 cut(s) 131, 384
BsiEI CGRYCG 1 cut(s) 132
BsiHKAI GWGCWC 3 cut(s) 536, 684, 1482
BsiHKCI CYCGRG 1 cut(s) 683
BsiSI CCGG 3 cut(s) 132, 286, 385
BslFI GGGAC 3 cut(s) 759, 1074, 1115
BslI CCNNNNNNNGG 3 cut(s) 285, 823, 1312
BsmFI GGGAC 3 cut(s) 759, 1074, 1115
BsmI GAATGC 4 cut(s) 238, 463, 1141, 1189
BsnI GGCC 1 cut(s) 908
BsoBI CYCGRG 1 cut(s) 683
Bsp119I TTCGAA 1 cut(s) 314
Bsp1286I GDGCHC 5 cut(s) 338, 536, 684, 989, 1482
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 2 cut(s) 1189, 1331
Bsp1720I GCTNAGC 1 cut(s) 111
BspACI CCGC 8 cut(s) 300, 302, 557, 589, 743, 909, 1419, 1491
BspANI GGCC 1 cut(s) 908
BspFNI CGCG 2 cut(s) 302, 437
BspLI GGNNCC 1 cut(s) 1488
BspMI ACCTGC 1 cut(s) 959
BspT104I TTCGAA 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 557
BsrDI GCAATG 2 cut(s) 526, 536
BsrFI RCCGGY 2 cut(s) 131, 384
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 1 cut(s) 1475
BssAI RCCGGY 2 cut(s) 131, 384
BssECI CCNNGG 1 cut(s) 300
BssMI GATC 2 cut(s) 1189, 1331
BssSI CACGAG 1 cut(s) 405
Bst2BI CACGAG 1 cut(s) 405
Bst2UI CCWGG 1 cut(s) 965
Bst4CI ACNGT 6 cut(s) 494, 508, 752, 823, 931, 1170
BstAUI TGTACA 1 cut(s) 957
BstBI TTCGAA 1 cut(s) 314
BstDEI CTNAG 1 cut(s) 111
BstDSI CCRYGG 1 cut(s) 300
BstF5I GGATG 1 cut(s) 1234
BstFNI CGCG 2 cut(s) 302, 437
BstH2I RGCGCY 1 cut(s) 672
BstHHI GCGC 2 cut(s) 439, 671
BstKTI GATC 2 cut(s) 1192, 1334
BstMBI GATC 2 cut(s) 1189, 1331
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 4 cut(s) 547, 563, 917, 1193
BstNI CCWGG 1 cut(s) 965
BstNSI RCATGY 1 cut(s) 472
BstSCI CCNGG 1 cut(s) 963
BstSFI CTRYAG 1 cut(s) 577
BstSLI GKGCMC 2 cut(s) 989, 1482
BstUI CGCG 2 cut(s) 302, 437
BstV1I GCAGC 3 cut(s) 205, 625, 1078
BstV2I GAAGAC 1 cut(s) 1262
BstX2I RGATCY 1 cut(s) 1331
BstYI RGATCY 1 cut(s) 1331
BsuRI GGCC 1 cut(s) 908
BtgI CCRYGG 1 cut(s) 300
BtsCI GGATG 1 cut(s) 1234
BtsIMutI CAGTG 5 cut(s) 734, 748, 1049, 1175, 1482
BveI ACCTGC 1 cut(s) 959
CaiI CAGNNNCTG 1 cut(s) 1506
CfoI GCGC 2 cut(s) 439, 671
Cfr10I RCCGGY 2 cut(s) 131, 384
Cfr13I GGNCC 1 cut(s) 58
Cfr42I CCGCGG 1 cut(s) 303
CseI GACGC 1 cut(s) 1267
Csp6I GTAC 5 cut(s) 283, 382, 490, 958, 1471
CspAI ACCGGT 2 cut(s) 131, 384
CviAII CATG 5 cut(s) 259, 469, 700, 1216, 1444
CviQI GTAC 5 cut(s) 283, 382, 490, 958, 1471
DdeI CTNAG 1 cut(s) 111
DpnI GATC 2 cut(s) 1191, 1333
DpnII GATC 2 cut(s) 1189, 1331
DriI GACNNNNNGTC 1 cut(s) 1172
Eam1105I GACNNNNNGTC 1 cut(s) 1172
Ecl136II GAGCTC 1 cut(s) 534
Eco24I GRGCYC 2 cut(s) 338, 536
Eco47I GGWCC 1 cut(s) 58
Eco47III AGCGCT 1 cut(s) 670
Eco53kI GAGCTC 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 683
EcoICRI GAGCTC 1 cut(s) 534
EcoO109I RGGNCCY 1 cut(s) 58
EcoRII CCWGG 1 cut(s) 963
EcoT38I GRGCYC 2 cut(s) 338, 536
FaeI CATG 5 cut(s) 262, 472, 703, 1219, 1447
FaqI GGGAC 3 cut(s) 759, 1074, 1115
FatI CATG 5 cut(s) 258, 468, 699, 1215, 1443
FauI CCCGC 2 cut(s) 736, 1498
Fnu4HI GCNGC 5 cut(s) 219, 590, 639, 909, 1092
FokI GGATG 1 cut(s) 1221
FriOI GRGCYC 2 cut(s) 338, 536
Fsp4HI GCNGC 5 cut(s) 219, 590, 639, 909, 1092
FspBI CTAG 4 cut(s) 714, 990, 1373, 1434
GlaI GCGC 2 cut(s) 438, 670
GluI GCNGC 5 cut(s) 219, 590, 639, 909, 1092
HaeII RGCGCY 1 cut(s) 672
HaeIII GGCC 1 cut(s) 908
HapII CCGG 3 cut(s) 132, 286, 385
HgaI GACGC 1 cut(s) 1267
HhaI GCGC 2 cut(s) 439, 671
Hin1II CATG 5 cut(s) 262, 472, 703, 1219, 1447
Hin6I GCGC 2 cut(s) 437, 669
HinP1I GCGC 2 cut(s) 437, 669
HindIII AAGCTT 2 cut(s) 113, 1270
HpaII CCGG 3 cut(s) 132, 286, 385
HphI GGTGA 2 cut(s) 268, 1373
Hpy166II GTNNAC 3 cut(s) 82, 958, 1480
Hpy188I TCNGA 8 cut(s) 9, 15, 93, 103, 177, 480, 1178, 1363
Hpy188III TCNNGA 6 cut(s) 41, 427, 654, 1132, 1238, 1343
Hpy8I GTNNAC 3 cut(s) 82, 958, 1480
HpyAV CCTTC 4 cut(s) 172, 360, 478, 898
HpyCH4III ACNGT 6 cut(s) 494, 508, 752, 823, 931, 1170
HpyCH4IV ACGT 2 cut(s) 561, 954
HpyF10VI GCNNNNNNNGC 4 cut(s) 547, 563, 917, 1193
HpyF3I CTNAG 1 cut(s) 111
HpySE526I ACGT 2 cut(s) 561, 954
Hsp92II CATG 5 cut(s) 262, 472, 703, 1219, 1447
HspAI GCGC 2 cut(s) 437, 669
KspI CCGCGG 1 cut(s) 303
Kzo9I GATC 2 cut(s) 1189, 1331
LmnI GCTCC 1 cut(s) 1298
Lsp1109I GCAGC 3 cut(s) 205, 625, 1078
LweI GCATC 1 cut(s) 1105
MaeI CTAG 4 cut(s) 714, 990, 1373, 1434
MaeII ACGT 2 cut(s) 561, 954
MaeIII GTNAC 3 cut(s) 392, 826, 931
MalI GATC 2 cut(s) 1191, 1333
MbiI CCGCTC 1 cut(s) 557
MboI GATC 2 cut(s) 1189, 1331
MboII GAAGA 8 cut(s) 328, 331, 457, 490, 814, 1267, 1328, 1409
MfeI CAATTG 2 cut(s) 208, 339
MflI RGATCY 1 cut(s) 1331
MhlI GDGCHC 5 cut(s) 338, 536, 684, 989, 1482
MluCI AATT 5 cut(s) 171, 208, 339, 659, 1400
MlyI GAGTC 5 cut(s) 314, 389, 521, 1056, 1434
MmeI TCCRAC 1 cut(s) 1407
MseI TTAA 5 cut(s) 117, 153, 624, 834, 1104
MslI CAYNNNNRTG 3 cut(s) 467, 1239, 1386
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 3 cut(s) 132, 286, 385
MspR9I CCNGG 1 cut(s) 965
MunI CAATTG 2 cut(s) 208, 339
Mva1269I GAATGC 4 cut(s) 238, 463, 1141, 1189
MvaI CCWGG 1 cut(s) 965
MvnI CGCG 2 cut(s) 302, 437
MwoI GCNNNNNNNGC 4 cut(s) 547, 563, 917, 1193
NdeII GATC 2 cut(s) 1189, 1331
NlaIII CATG 5 cut(s) 262, 472, 703, 1219, 1447
NlaIV GGNNCC 1 cut(s) 1488
NmuCI GTSAC 1 cut(s) 392
NspI RCATGY 1 cut(s) 472
NspV TTCGAA 1 cut(s) 314
OliI CACNNNNGTG 1 cut(s) 1386
PaeR7I CTCGAG 1 cut(s) 683
PciI ACATGT 1 cut(s) 468
PctI GAATGC 4 cut(s) 238, 463, 1141, 1189
PflMI CCANNNNNTGG 1 cut(s) 1312
PinAI ACCGGT 2 cut(s) 131, 384
PkrI GCNGC 5 cut(s) 220, 591, 640, 910, 1093
PleI GAGTC 5 cut(s) 313, 389, 520, 1056, 1434
PpsI GAGTC 5 cut(s) 313, 389, 520, 1056, 1434
PpuMI RGGWCCY 1 cut(s) 58
PscI ACATGT 1 cut(s) 468
PsiI TTATAA 1 cut(s) 1044
Psp124BI GAGCTC 1 cut(s) 536
Psp5II RGGWCCY 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 963
PspGI CCWGG 1 cut(s) 963
PspN4I GGNNCC 1 cut(s) 1488
PspPI GGNCC 1 cut(s) 58
PspPPI RGGWCCY 1 cut(s) 58
PspXI VCTCGAGB 1 cut(s) 683
PsrI GAACNNNNNNTAC 2 cut(s) 275, 307
PstNI CAGNNNCTG 1 cut(s) 1506
PsuI RGATCY 1 cut(s) 1331
RsaI GTAC 5 cut(s) 284, 383, 491, 959, 1472
RsaNI GTAC 5 cut(s) 283, 382, 490, 958, 1471
RseI CAYNNNNRTG 3 cut(s) 467, 1239, 1386
SacI GAGCTC 1 cut(s) 536
SacII CCGCGG 1 cut(s) 303
SaqAI TTAA 5 cut(s) 117, 153, 624, 834, 1104
SatI GCNGC 5 cut(s) 219, 590, 639, 909, 1092
Sau3AI GATC 2 cut(s) 1189, 1331
Sau96I GGNCC 1 cut(s) 58
SchI GAGTC 5 cut(s) 314, 389, 521, 1056, 1434
ScrFI CCNGG 1 cut(s) 965
SduI GDGCHC 5 cut(s) 338, 536, 684, 989, 1482
SfaNI GCATC 1 cut(s) 1105
SfcI CTRYAG 1 cut(s) 577
Sfr274I CTCGAG 1 cut(s) 683
Sfr303I CCGCGG 1 cut(s) 303
SfuI TTCGAA 1 cut(s) 314
SgrBI CCGCGG 1 cut(s) 303
SinI GGWCC 1 cut(s) 58
SlaI CTCGAG 1 cut(s) 683
SmiMI CAYNNNNRTG 3 cut(s) 467, 1239, 1386
SmlI CTYRAG 3 cut(s) 197, 683, 1449
SmoI CTYRAG 3 cut(s) 197, 683, 1449
Sse9I AATT 5 cut(s) 171, 208, 339, 659, 1400
SsiI CCGC 8 cut(s) 300, 302, 557, 589, 743, 909, 1419, 1491
SspMI CTAG 4 cut(s) 714, 990, 1373, 1434
SstI GAGCTC 1 cut(s) 536
StyD4I CCNGG 1 cut(s) 963
TaaI ACNGT 6 cut(s) 494, 508, 752, 823, 931, 1170
TaiI ACGT 2 cut(s) 564, 957
TaqI TCGA 6 cut(s) 128, 223, 246, 314, 684, 999
TasI AATT 5 cut(s) 171, 208, 339, 659, 1400
TatI WGTACW 1 cut(s) 957
TauI GCSGC 2 cut(s) 592, 911
Tru1I TTAA 5 cut(s) 117, 153, 624, 834, 1104
Tru9I TTAA 5 cut(s) 117, 153, 624, 834, 1104
TscAI CASTG 5 cut(s) 741, 755, 1056, 1175, 1482
TseFI GTSAC 1 cut(s) 392
TseI GCWGC 3 cut(s) 218, 638, 1091
Tsp45I GTSAC 1 cut(s) 392
TspDTI ATGAA 3 cut(s) 17, 497, 917
TspRI CASTG 5 cut(s) 741, 755, 1056, 1175, 1482
Van91I CCANNNNNTGG 1 cut(s) 1312
VneI GTGCAC 1 cut(s) 1478
VpaK11BI GGWCC 1 cut(s) 58
XapI RAATTY 1 cut(s) 171
XceI RCATGY 1 cut(s) 472
XhoI CTCGAG 1 cut(s) 683
XspI CTAG 4 cut(s) 714, 990, 1373, 1434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.