Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0008589

Physical Location & Seq

Genomic Coordinates Forward (+)

1225159 .. 1226769

Gene Structure 1611 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G000590.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1611 bp

ATGAATAAACTCCCATTTCGTCAAGTTTCAATCTTGCCCAATTCAATTGCTAAAGAACCCACCTTTACAAAACCCTCAGTTTCTGTGAATTTGGAAACAAGGAATTGGAATCCAATTCCTATTCCCCACAGAACAATTCCTGAACCCAAAGGCCAAGACCTTGATTTTGTTAATGTTGTAAACAGCCATTTGATTCACTCTGACTGGGCTAAGCTTGTTTCCTTAGCCAATGGTCTGACTGCTTTCAGAGTGAAACATATTTTGCTGAAAATTCAGAAGGATTATGTTCTTTCTCTTGAGTTTTTCAACTGGGTTGGAACTCACAAACCCACTTCACATACCCTCGAAACACATTCCATGATCCTCCACATTCTCACCAAGTATCGAAAATTTAAGTCTGCAGAGTCGATTTTGAGAAAGATTCTTGTGCCAGGGTCCATAGACTTGCCCCCCAAGCTGTTTGAAGCGATACTGTATTCGTACCGCTTGTGTGATTCCTCTCCTCGGGTTTTTGATTCGCTTTTCAAGACGTTTGCGCATATGAAGAAGTTTAGGAATGCCACAGACACGTTTTGTAGAATGAAGGATTACGGGTTTTACCCGACGGTTGAGTCATGCAATGCGTATTTGAGCTCATTGCTTGGTTTACATAGAGCTGATGTTGCTTTGGCATTCTACAGAGAAATGAGGCGTTGTCGAATTTCACCCAATGTTTATACTCTTAATATGGTTATGTGTGCTTATTGTAGAGTGGGAAAATTAGAGAATGCTGTTGAAGTCCTTGAGAAGATGGAGAGCATGGGTTTCAGTCCTACCATTGTGTCATATAATACACTTATTGCAGGACATTGCGATAAGGGTCTTCTGAGTGCCGCTGTGAAGTTCAAAAACTTGATGGTGAAGAATGGATTGCACCCGAATGTGGTAACTTTTAACACACTCATTGATGGGTTTTGTAAGCAAGGAAAACTACCAGAAGCAAATAGAATTTTTAGTGAGATGAAAGCCCTGAATGTGGCTCCTAATACAGTAACCTATAATACCATGATAAATGGGTATGGTCAAGCAGGTAATAGTGAGATGGGCAGTAGGCTTTTTGAGGAGATGTCAAAGGACCGAGTCAGCGCTGATATTCTGACTTATAATGGATTGATTTTGGGACTATGCAAGGAGGGTAAGACAAAGAAAGCTGCATATCTGGTAAAAGAGCTTGACAGAAAGAACTTTGTCCCAAATGCCTCAACCTTTTCTGCACTTGTTGGTGGGCAATGTGTGAGAAAGAACTCAGATCGTGCCTTTCTGTTGTACAAGAGCATGGTTAGGAGTAGCTATCATCCCGATGAACATACTTTTAAGATGTTGTTGTCTAGCTTCTGCAATAATAGGGATTTTGATGGAGCAGTTGAAGTCTTGAGAGAAATGTTTGAGAGAAATTTTGTTCCTGATTCAGGCATATTATCTGATCTATGTCTGGGACTTCAACGCTGTGGAAAAGAAAAATGGGTAGAATTGCTATGCACTGAGATGGAAGCTAGACGCCTCATTCCTCAAGGTTTTGACATGGCGAAGATTATCTGCTGTGTAGAAGAGAATGATAACTTAACAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000959 GO:0000963 GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0010467 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0090615 GO:0090617 GO:0097159 GO:0140053 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

536

Amino Acids

60.82

Weight (kDa)

9.04

Isoelectric Point (pI)

31.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_long	PF17177	181 - 281	4.3e-07	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	201 - 245	2.9e-06	PPR repeat family
PPR_3	PF13812	227 - 280	5.1e-11	Pentatricopeptide repeat domain
PPR_1	PF12854	234 - 265	9.8e-09	PPR repeat
PPR_2	PF13041	236 - 274	1.8e-08	PPR repeat family
PPR	PF01535	240 - 269	7.6e-06	PPR repeat
PPR_1	PF12854	267 - 300	7e-08	PPR repeat
PPR_2	PF13041	271 - 320	7e-18	PPR repeat family
PPR_3	PF13812	299 - 355	8.8e-14	Pentatricopeptide repeat domain
PPR_1	PF12854	302 - 335	2.1e-14	PPR repeat
PPR_2	PF13041	306 - 353	1e-19	PPR repeat family
PPR	PF01535	309 - 338	2.1e-08	PPR repeat
PPR_1	PF12854	338 - 370	1.4e-08	PPR repeat
PPR_2	PF13041	341 - 390	1.8e-15	PPR repeat family
PPR	PF01535	344 - 373	2.1e-08	PPR repeat
PPR_3	PF13812	364 - 421	3.6e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	374 - 403	6.3e-06	PPR repeat
PPR_2	PF13041	377 - 424	9.2e-10	PPR repeat family
PPR_2	PF13041	419 - 460	2e-06	PPR repeat family
PPR_1	PF12854	444 - 474	2.4e-06	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008589)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G26680 AT4G26680
fragaria_vesca	FvH4_6g40750
malus_domestica	MD17G1115600.v1.1
prunus_persica	Prupe.3G199000_v2.0.a1 Prupe.3G199000_v2.0.a1
pyrus_communis	pycom17g10680
rosa_chinensis	RchiOBHm_Chr2g0156201 RchiOBHm_Chr4g0385191
rosa_laevigata	RLG00000010250 RLG00000020895
rosa_multiflora	Rmu_sc0000260.1_g000011 Rmu_sc0003086.1_g000003
rosa_roxburghii	Rroxscaffold_2G00093150 Rroxscaffold_5G00333090
rosa_rugosa	Rorug02G0452500
rosa_samantha	Rh2AG519000 Rh2BG530400 Rh2CG504000 Rh2DG539400 Rh4CG003600
rosa_wichuraiana	Rw2G042750 Rw4G000590

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1143
AasI	GACNNNNNNGTC	1 cut(s)	610
Acc16I	TGCGCA	1 cut(s)	537
Acc36I	ACCTGC	1 cut(s)	1058
AciI	CCGC	2 cut(s)	484, 873
AclWI	GGATC	1 cut(s)	355
AcsI	RAATTY	6 cut(s)	88, 270, 389, 699, 987, 1432
AcyI	GRCGYC	1 cut(s)	1537
AfaI	GTAC	2 cut(s)	482, 1307
AfeI	AGCGCT	1 cut(s)	1126
AfiI	CCNNNNNNNGG	4 cut(s)	504, 922, 1015, 1448
AflIII	ACRYGT	1 cut(s)	567
AgsI	TTSAA	9 cut(s)	30, 45, 307, 464, 526, 776, 886, 1406, 1481
AjnI	CCWGG	1 cut(s)	430
AluBI	AGCT	9 cut(s)	214, 457, 633, 656, 1190, 1210, 1329, 1371, 1532
AluI	AGCT	9 cut(s)	214, 457, 633, 656, 1190, 1210, 1329, 1371, 1532
Alw21I	GWGCWC	1 cut(s)	635
AlwI	GGATC	1 cut(s)	355
AlwNI	CAGNNNCTG	1 cut(s)	83
Ama87I	CYCGRG	1 cut(s)	504
Aor51HI	AGCGCT	1 cut(s)	1126
AoxI	GGCC	1 cut(s)	151
ApeKI	GCWGC	1 cut(s)	1190
ApoI	RAATTY	6 cut(s)	88, 270, 389, 699, 987, 1432
ArsI	GACNNNNNNTTYG	2 cut(s)	149, 181
AspLEI	GCGC	2 cut(s)	538, 1127
AspS9I	GGNCC	2 cut(s)	435, 1114
AsuHPI	GGTGA	3 cut(s)	367, 696, 910
AvaI	CYCGRG	1 cut(s)	504
AvaII	GGWCC	2 cut(s)	435, 1114
BanII	GRGCYC	1 cut(s)	635
BbsI	GAAGAC	1 cut(s)	854
Bbv12I	GWGCWC	1 cut(s)	635
BbvI	GCAGC	1 cut(s)	1177
BccI	CCATC	6 cut(s)	784, 889, 941, 1075, 1388, 1519
BciT130I	CCWGG	1 cut(s)	432
BfaI	CTAG	2 cut(s)	1368, 1533
BfmI	CTRYAG	2 cut(s)	399, 676
BfoI	RGCGCY	1 cut(s)	1128
BfuAI	ACCTGC	1 cut(s)	1058
BisI	GCNGC	2 cut(s)	873, 1191
BlpI	GCTNAGC	1 cut(s)	210
BlsI	GCNGC	2 cut(s)	874, 1192
Bme1390I	CCNGG	1 cut(s)	432
Bme18I	GGWCC	2 cut(s)	435, 1114
BmeT110I	CYCGRG	1 cut(s)	504
BmgT120I	GGNCC	2 cut(s)	435, 1114
BmiI	GGNNCC	2 cut(s)	436, 1020
BmrFI	CCNGG	1 cut(s)	432
BmrI	ACTGGG	2 cut(s)	214, 319
BmuI	ACTGGG	2 cut(s)	214, 319
BpiI	GAAGAC	1 cut(s)	854
Bpu10I	CCTNAGC	1 cut(s)	223
Bpu1102I	GCTNAGC	1 cut(s)	210
BpuEI	CTTGAG	4 cut(s)	317, 803, 1432, 1533
BsaHI	GRCGYC	1 cut(s)	1537
BsaJI	CCNNGG	2 cut(s)	431, 503
Bsc4I	CCNNNNNNNGG	4 cut(s)	504, 922, 1015, 1448
Bse1I	ACTGG	2 cut(s)	209, 314
Bse3DI	GCAATG	4 cut(s)	625, 635, 847, 1274
BseBI	CCWGG	1 cut(s)	432
BseDI	CCNNGG	2 cut(s)	431, 503
BseGI	GGATG	1 cut(s)	1333
BseLI	CCNNNNNNNGG	4 cut(s)	504, 922, 1015, 1448
BseMI	GCAATG	4 cut(s)	625, 635, 847, 1274
BseMII	CTCAG	4 cut(s)	90, 857, 1299, 1512
BseNI	ACTGG	2 cut(s)	209, 314
BseRI	GAGGAG	2 cut(s)	492, 1115
BseXI	GCAGC	1 cut(s)	1177
BsgI	GTGCAG	1 cut(s)	1236
BshFI	GGCC	1 cut(s)	153
BsiHKAI	GWGCWC	1 cut(s)	635
BsiHKCI	CYCGRG	1 cut(s)	504
BslFI	GGGAC	3 cut(s)	1173, 1214, 1488
BslI	CCNNNNNNNGG	4 cut(s)	504, 922, 1015, 1448
BsmFI	GGGAC	3 cut(s)	1173, 1214, 1488
BsmI	GAATGC	3 cut(s)	562, 671, 772
BsnI	GGCC	1 cut(s)	153
BsoBI	CYCGRG	1 cut(s)	504
Bsp1286I	GDGCHC	1 cut(s)	635
Bsp1407I	TGTACA	1 cut(s)	1305
Bsp143I	GATC	3 cut(s)	360, 1288, 1462
Bsp1720I	GCTNAGC	1 cut(s)	210
BspACI	CCGC	2 cut(s)	484, 873
BspANI	GGCC	1 cut(s)	153
BspCNI	CTCAG	4 cut(s)	89, 858, 1298, 1513
BspLI	GGNNCC	2 cut(s)	436, 1020
BspMAI	CTGCAG	1 cut(s)	403
BspMI	ACCTGC	1 cut(s)	1058
BspPI	GGATC	1 cut(s)	355
BsrDI	GCAATG	4 cut(s)	625, 635, 847, 1274
BsrGI	TGTACA	1 cut(s)	1305
BsrI	ACTGG	2 cut(s)	209, 314
BssECI	CCNNGG	2 cut(s)	431, 503
BssMI	GATC	3 cut(s)	360, 1288, 1462
BssNI	GRCGYC	1 cut(s)	1537
Bst2UI	CCWGG	1 cut(s)	432
Bst4CI	ACNGT	3 cut(s)	474, 607, 1030
Bst6I	CTCTTC	1 cut(s)	1581
BstACI	GRCGYC	1 cut(s)	1537
BstAUI	TGTACA	1 cut(s)	1305
BstDEI	CTNAG	6 cut(s)	76, 210, 223, 866, 1285, 1521
BstENI	CCTNNNNNAGG	1 cut(s)	1446
BstF5I	GGATG	1 cut(s)	1333
BstH2I	RGCGCY	1 cut(s)	1128
BstHHI	GCGC	2 cut(s)	538, 1127
BstKTI	GATC	3 cut(s)	363, 1291, 1465
BstMBI	GATC	3 cut(s)	360, 1288, 1462
BstMWI	GCNNNNNNNGC	2 cut(s)	454, 662
BstNI	CCWGG	1 cut(s)	432
BstSCI	CCNGG	1 cut(s)	430
BstSFI	CTRYAG	2 cut(s)	399, 676
BstV1I	GCAGC	1 cut(s)	1177
BstV2I	GAAGAC	1 cut(s)	854
BsuRI	GGCC	1 cut(s)	153
BtsCI	GGATG	1 cut(s)	1333
BtsIMutI	CAGTG	1 cut(s)	1518
BveI	ACCTGC	1 cut(s)	1058
CaiI	CAGNNNCTG	1 cut(s)	83
CfoI	GCGC	2 cut(s)	538, 1127
Cfr13I	GGNCC	2 cut(s)	435, 1114
CseI	GACGC	1 cut(s)	1545
Csp6I	GTAC	2 cut(s)	481, 1306
CviAII	CATG	6 cut(s)	358, 615, 799, 1045, 1315, 1561
CviQI	GTAC	2 cut(s)	481, 1306
DdeI	CTNAG	6 cut(s)	76, 210, 223, 866, 1285, 1521
DpnI	GATC	3 cut(s)	362, 1290, 1464
DpnII	GATC	3 cut(s)	360, 1288, 1462
DrdI	GACNNNNNNGTC	1 cut(s)	610
DseDI	GACNNNNNNGTC	1 cut(s)	610
Eam1104I	CTCTTC	1 cut(s)	1581
EarI	CTCTTC	1 cut(s)	1581
Ecl136II	GAGCTC	1 cut(s)	633
Eco24I	GRGCYC	1 cut(s)	635
Eco47I	GGWCC	2 cut(s)	435, 1114
Eco47III	AGCGCT	1 cut(s)	1126
Eco53kI	GAGCTC	1 cut(s)	633
Eco88I	CYCGRG	1 cut(s)	504
EcoICRI	GAGCTC	1 cut(s)	633
EcoNI	CCTNNNNNAGG	1 cut(s)	1446
EcoRII	CCWGG	1 cut(s)	430
EcoT38I	GRGCYC	1 cut(s)	635
FaeI	CATG	6 cut(s)	361, 618, 802, 1048, 1318, 1564
FaqI	GGGAC	3 cut(s)	1173, 1214, 1488
FatI	CATG	6 cut(s)	357, 614, 798, 1044, 1314, 1560
FauNDI	CATATG	1 cut(s)	540
Fnu4HI	GCNGC	2 cut(s)	873, 1191
FokI	GGATG	1 cut(s)	1320
FriOI	GRGCYC	1 cut(s)	635
Fsp4HI	GCNGC	2 cut(s)	873, 1191
FspBI	CTAG	2 cut(s)	1368, 1533
FspI	TGCGCA	1 cut(s)	537
GlaI	GCGC	2 cut(s)	537, 1126
GluI	GCNGC	2 cut(s)	873, 1191
HaeII	RGCGCY	1 cut(s)	1128
HaeIII	GGCC	1 cut(s)	153
HgaI	GACGC	1 cut(s)	1545
HhaI	GCGC	2 cut(s)	538, 1127
Hin1I	GRCGYC	1 cut(s)	1537
Hin1II	CATG	6 cut(s)	361, 618, 802, 1048, 1318, 1564
Hin6I	GCGC	2 cut(s)	536, 1125
HinP1I	GCGC	2 cut(s)	536, 1125
HindIII	AAGCTT	1 cut(s)	212
HinfI	GANTC	9 cut(s)	109, 193, 404, 421, 494, 515, 611, 1119, 1445
HphI	GGTGA	3 cut(s)	367, 696, 910
Hpy166II	GTNNAC	2 cut(s)	181, 647
Hpy188I	TCNGA	8 cut(s)	202, 237, 248, 276, 867, 1137, 1288, 1462
Hpy188III	TCNNGA	6 cut(s)	140, 296, 526, 1337, 1411, 1442
Hpy8I	GTNNAC	2 cut(s)	181, 647
Hpy99I	CGWCG	1 cut(s)	607
HpyAV	CCTTC	2 cut(s)	271, 577
HpyCH4III	ACNGT	3 cut(s)	474, 607, 1030
HpyCH4IV	ACGT	2 cut(s)	530, 569
HpyCH4V	TGCA	9 cut(s)	401, 618, 842, 913, 1167, 1193, 1253, 1377, 1518
HpyF10VI	GCNNNNNNNGC	2 cut(s)	454, 662
HpyF3I	CTNAG	6 cut(s)	76, 210, 223, 866, 1285, 1521
HpySE526I	ACGT	2 cut(s)	530, 569
Hsp92I	GRCGYC	1 cut(s)	1537
Hsp92II	CATG	6 cut(s)	361, 618, 802, 1048, 1318, 1564
HspAI	GCGC	2 cut(s)	536, 1125
Kzo9I	GATC	3 cut(s)	360, 1288, 1462
LmnI	GCTCC	2 cut(s)	1024, 1397
Lsp1109I	GCAGC	1 cut(s)	1177
MaeI	CTAG	2 cut(s)	1368, 1533
MaeII	ACGT	2 cut(s)	530, 569
MaeIII	GTNAC	2 cut(s)	925, 1030
MalI	GATC	3 cut(s)	362, 1290, 1464
MboI	GATC	3 cut(s)	360, 1288, 1462
MboII	GAAGA	6 cut(s)	556, 799, 854, 913, 1579, 1598
MfeI	CAATTG	1 cut(s)	45
MhlI	GDGCHC	1 cut(s)	635
MlyI	GAGTC	3 cut(s)	413, 620, 1128
MmeI	TCCRAC	1 cut(s)	295
MseI	TTAA	6 cut(s)	171, 393, 723, 933, 1353, 1601
MslI	CAYNNNNRTG	3 cut(s)	918, 1338, 1523
MspA1I	CMGCKG	1 cut(s)	875
MspR9I	CCNGG	1 cut(s)	432
MunI	CAATTG	1 cut(s)	45
Mva1269I	GAATGC	3 cut(s)	562, 671, 772
MvaI	CCWGG	1 cut(s)	432
MwoI	GCNNNNNNNGC	2 cut(s)	454, 662
NdeI	CATATG	1 cut(s)	540
NdeII	GATC	3 cut(s)	360, 1288, 1462
NlaIII	CATG	6 cut(s)	361, 618, 802, 1048, 1318, 1564
NlaIV	GGNNCC	2 cut(s)	436, 1020
NsbI	TGCGCA	1 cut(s)	537
PctI	GAATGC	3 cut(s)	562, 671, 772
PfeI	GAWTC	6 cut(s)	109, 193, 421, 494, 515, 1445
PflFI	GACNNNGTC	1 cut(s)	1118
PkrI	GCNGC	2 cut(s)	874, 1192
PleI	GAGTC	3 cut(s)	412, 619, 1127
PpsI	GAGTC	3 cut(s)	412, 619, 1127
PsiI	TTATAA	1 cut(s)	1143
Psp124BI	GAGCTC	1 cut(s)	635
Psp6I	CCWGG	1 cut(s)	430
PspGI	CCWGG	1 cut(s)	430
PspN4I	GGNNCC	2 cut(s)	436, 1020
PspPI	GGNCC	2 cut(s)	435, 1114
PstI	CTGCAG	1 cut(s)	403
PstNI	CAGNNNCTG	1 cut(s)	83
PsyI	GACNNNGTC	1 cut(s)	1118
RsaI	GTAC	2 cut(s)	482, 1307
RsaNI	GTAC	2 cut(s)	481, 1306
RseI	CAYNNNNRTG	3 cut(s)	918, 1338, 1523
SacI	GAGCTC	1 cut(s)	635
SaqAI	TTAA	6 cut(s)	171, 393, 723, 933, 1353, 1601
SatI	GCNGC	2 cut(s)	873, 1191
Sau3AI	GATC	3 cut(s)	360, 1288, 1462
Sau96I	GGNCC	2 cut(s)	435, 1114
SchI	GAGTC	3 cut(s)	413, 620, 1128
ScrFI	CCNGG	1 cut(s)	432
SduI	GDGCHC	1 cut(s)	635
SfcI	CTRYAG	2 cut(s)	399, 676
SinI	GGWCC	2 cut(s)	435, 1114
SmiMI	CAYNNNNRTG	3 cut(s)	918, 1338, 1523
SmlI	CTYRAG	4 cut(s)	296, 782, 1411, 1548
SmoI	CTYRAG	4 cut(s)	296, 782, 1411, 1548
SsiI	CCGC	2 cut(s)	484, 873
SspMI	CTAG	2 cut(s)	1368, 1533
SstI	GAGCTC	1 cut(s)	635
StyD4I	CCNGG	1 cut(s)	430
TaaI	ACNGT	3 cut(s)	474, 607, 1030
TaiI	ACGT	2 cut(s)	533, 572
TaqI	TCGA	4 cut(s)	345, 385, 407, 697
TaqII	GACCGA	1 cut(s)	1131
TatI	WGTACW	1 cut(s)	1305
TauI	GCSGC	1 cut(s)	875
TfiI	GAWTC	6 cut(s)	109, 193, 421, 494, 515, 1445
Tru1I	TTAA	6 cut(s)	171, 393, 723, 933, 1353, 1601
Tru9I	TTAA	6 cut(s)	171, 393, 723, 933, 1353, 1601
TscAI	CASTG	1 cut(s)	1525
TseI	GCWGC	1 cut(s)	1190
TspDTI	ATGAA	5 cut(s)	17, 557, 596, 1016, 1356
TspRI	CASTG	1 cut(s)	1525
Tth111I	GACNNNGTC	1 cut(s)	1118
VpaK11BI	GGWCC	2 cut(s)	435, 1114
XagI	CCTNNNNNAGG	1 cut(s)	1446
XapI	RAATTY	6 cut(s)	88, 270, 389, 699, 987, 1432
XspI	CTAG	2 cut(s)	1368, 1533

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.