FvH4_6g41440

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009447
Physical Location & Seq
Reverse (-)
32578529 .. 32580919
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g41440.t1

Sequence Viewer

Length: 564 bp
ATGAGGCAAAATATCCAAAACATGAGGAAAAGCCCCAGAGTAGCAGATGAGAGCATGGTTAACATAGAACGCGGGAACTTAGCCGAAGAATTGCAGATTTATGGAAGATCATCATCCCGCGGTTGGAAGGGGTTTTCGGTTATAGTTAGCGTTGTGATCCTGCCATTTTCTGTTCTTTCTTGCTTCTCTCAGCCTCATGTTCATGGCGCTGATGGGGTTTGGACCTCGGGTGATTTTGCGCAGATAACGGAGATAAATCATCTAATGGTAAGTGACAGCATGCGCTATGCCATCTTGATAAAGGTGGGACTAGGGGCTATGTTAAGGGATAGTAAAGGTGAAACGCTGCTGGCAGTGGGTGAAAAAATTCAAGGGGATCTCCAGCCTTGTGCAGCTGAATTGGCTGCTTTAATTTTTGGGTTGGAATCGGTAAGGAGGAGGAATGGTACATTGGAGGGGATGGAGAGTGATTGTCTGGGTGTAGTGGATATGGTTAATGCAGGTGAGAAGTGCTTGAATGCAGAAGGGGTCTTAGTACATAAAGTCATACTCTCTGTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

20.33

Weight (kDa)

5.61

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_3 PF13456 103 - 171 1.9e-07 Reverse transcriptase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 491
Acc16I TGCGCA 1 cut(s) 240
Acc36I ACCTGC 1 cut(s) 491
AccII CGCG 2 cut(s) 72, 120
AciI CCGC 3 cut(s) 72, 118, 120
AclWI GGATC 2 cut(s) 151, 384
AcsI RAATTY 1 cut(s) 366
AfaI GTAC 2 cut(s) 448, 537
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 3 cut(s) 371, 517, 560
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AluBI AGCT 1 cut(s) 395
AluI AGCT 1 cut(s) 395
AlwI GGATC 2 cut(s) 151, 384
Ama87I CYCGRG 1 cut(s) 226
ApeKI GCWGC 3 cut(s) 346, 392, 404
ApoI RAATTY 1 cut(s) 366
Asp700I GAANNNNTTC 1 cut(s) 366
AspLEI GCGC 3 cut(s) 209, 241, 285
AspS9I GGNCC 1 cut(s) 222
AsuHPI GGTGA 4 cut(s) 242, 350, 371, 515
AvaI CYCGRG 1 cut(s) 226
AvaII GGWCC 1 cut(s) 222
BbvI GCAGC 3 cut(s) 333, 391, 404
BccI CCATC 3 cut(s) 206, 299, 454
BfaI CTAG 1 cut(s) 311
BfoI RGCGCY 1 cut(s) 210
BfuAI ACCTGC 1 cut(s) 491
BisI GCNGC 3 cut(s) 347, 393, 405
BlsI GCNGC 3 cut(s) 348, 394, 406
Bme18I GGWCC 1 cut(s) 222
BmeT110I CYCGRG 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 222
BpmI CTGGAG 1 cut(s) 365
BsaBI GATNNNNATC 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 118, 225
BsaXI ACNNNNNCTCC 2 cut(s) 430, 460
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse8I GATNNNNATC 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 118, 225
BseGI GGATG 2 cut(s) 113, 465
BseJI GATNNNNATC 1 cut(s) 112
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMII CTCAG 1 cut(s) 203
BseRI GAGGAG 1 cut(s) 451
BseXI GCAGC 3 cut(s) 333, 391, 404
BsgI GTGCAG 1 cut(s) 411
Bsh1236I CGCG 2 cut(s) 72, 120
BsiHKCI CYCGRG 1 cut(s) 226
BslFI GGGAC 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 123
BsmFI GGGAC 1 cut(s) 321
BsmI GAATGC 1 cut(s) 523
BsoBI CYCGRG 1 cut(s) 226
Bsp143I GATC 3 cut(s) 107, 156, 376
BspACI CCGC 3 cut(s) 72, 118, 120
BspCNI CTCAG 1 cut(s) 202
BspFNI CGCG 2 cut(s) 72, 120
BspMI ACCTGC 1 cut(s) 491
BspPI GGATC 2 cut(s) 151, 384
BssECI CCNNGG 2 cut(s) 118, 225
BssMI GATC 3 cut(s) 107, 156, 376
BstC8I GCNNGC 2 cut(s) 281, 351
BstDEI CTNAG 3 cut(s) 79, 189, 532
BstDSI CCRYGG 1 cut(s) 118
BstF5I GGATG 2 cut(s) 113, 465
BstFNI CGCG 2 cut(s) 72, 120
BstH2I RGCGCY 1 cut(s) 210
BstHHI GCGC 3 cut(s) 209, 241, 285
BstKTI GATC 3 cut(s) 110, 159, 379
BstMBI GATC 3 cut(s) 107, 156, 376
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNSI RCATGY 1 cut(s) 283
BstUI CGCG 2 cut(s) 72, 120
BstV1I GCAGC 3 cut(s) 333, 391, 404
BstX2I RGATCY 1 cut(s) 376
BstYI RGATCY 1 cut(s) 376
BtgI CCRYGG 1 cut(s) 118
BtsCI GGATG 2 cut(s) 113, 465
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 1 cut(s) 360
BveI ACCTGC 1 cut(s) 491
Cac8I GCNNGC 2 cut(s) 281, 351
CfoI GCGC 3 cut(s) 209, 241, 285
Cfr13I GGNCC 1 cut(s) 222
Cfr42I CCGCGG 1 cut(s) 121
Csp6I GTAC 2 cut(s) 447, 536
CviAII CATG 5 cut(s) 22, 55, 197, 203, 280
CviJI RGCY 7 cut(s) 33, 83, 193, 317, 385, 395, 404
CviKI_1 RGCY 7 cut(s) 33, 83, 193, 317, 385, 395, 404
CviQI GTAC 2 cut(s) 447, 536
DdeI CTNAG 3 cut(s) 79, 189, 532
DpnI GATC 3 cut(s) 109, 158, 378
DpnII GATC 3 cut(s) 107, 156, 376
Eco47I GGWCC 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 226
FaeI CATG 5 cut(s) 25, 58, 200, 206, 283
FaqI GGGAC 1 cut(s) 321
FatI CATG 5 cut(s) 21, 54, 196, 202, 279
FauI CCCGC 2 cut(s) 65, 125
Fnu4HI GCNGC 3 cut(s) 347, 393, 405
FokI GGATG 2 cut(s) 100, 472
Fsp4HI GCNGC 3 cut(s) 347, 393, 405
FspBI CTAG 1 cut(s) 311
FspI TGCGCA 1 cut(s) 240
GlaI GCGC 3 cut(s) 208, 240, 284
GluI GCNGC 3 cut(s) 347, 393, 405
GsuI CTGGAG 1 cut(s) 365
HaeII RGCGCY 1 cut(s) 210
HhaI GCGC 3 cut(s) 209, 241, 285
Hin1II CATG 5 cut(s) 25, 58, 200, 206, 283
Hin6I GCGC 3 cut(s) 207, 239, 283
HinP1I GCGC 3 cut(s) 207, 239, 283
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 1 cut(s) 425
HpaI GTTAAC 1 cut(s) 61
HphI GGTGA 4 cut(s) 242, 350, 371, 515
Hpy166II GTNNAC 1 cut(s) 61
Hpy188III TCNNGA 1 cut(s) 295
Hpy8I GTNNAC 1 cut(s) 61
HpyAV CCTTC 2 cut(s) 121, 518
HpyCH4V TGCA 4 cut(s) 94, 392, 500, 521
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 3 cut(s) 79, 189, 532
Hsp92II CATG 5 cut(s) 25, 58, 200, 206, 283
HspAI GCGC 3 cut(s) 207, 239, 283
KspAI GTTAAC 1 cut(s) 61
KspI CCGCGG 1 cut(s) 121
Kzo9I GATC 3 cut(s) 107, 156, 376
LpnPI CCDG 6 cut(s) 49, 173, 335, 395, 461, 486
Lsp1109I GCAGC 3 cut(s) 333, 391, 404
MaeI CTAG 1 cut(s) 311
MaeIII GTNAC 1 cut(s) 272
MalI GATC 3 cut(s) 109, 158, 378
MboI GATC 3 cut(s) 107, 156, 376
MboII GAAGA 2 cut(s) 98, 117
MflI RGATCY 1 cut(s) 376
MluCI AATT 4 cut(s) 89, 366, 398, 411
MmeI TCCRAC 2 cut(s) 104, 402
MnlI CCTC 6 cut(s) 18, 204, 235, 429, 432, 448
MroXI GAANNNNTTC 1 cut(s) 366
MseI TTAA 4 cut(s) 60, 323, 410, 495
MslI CAYNNNNRTG 1 cut(s) 201
MspA1I CMGCKG 2 cut(s) 120, 395
Mva1269I GAATGC 1 cut(s) 523
MvnI CGCG 2 cut(s) 72, 120
MwoI GCNNNNNNNGC 1 cut(s) 401
NdeII GATC 3 cut(s) 107, 156, 376
NlaIII CATG 5 cut(s) 25, 58, 200, 206, 283
NmuCI GTSAC 1 cut(s) 272
NsbI TGCGCA 1 cut(s) 240
NspI RCATGY 1 cut(s) 283
PaeI GCATGC 1 cut(s) 283
PaqCI CACCTGC 1 cut(s) 491
PctI GAATGC 1 cut(s) 523
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 425
PkrI GCNGC 3 cut(s) 348, 394, 406
PspPI GGNCC 1 cut(s) 222
PsuI RGATCY 1 cut(s) 376
PvuII CAGCTG 1 cut(s) 395
RsaI GTAC 2 cut(s) 448, 537
RsaNI GTAC 2 cut(s) 447, 536
RseI CAYNNNNRTG 1 cut(s) 201
SacII CCGCGG 1 cut(s) 121
SaqAI TTAA 4 cut(s) 60, 323, 410, 495
SatI GCNGC 3 cut(s) 347, 393, 405
Sau3AI GATC 3 cut(s) 107, 156, 376
Sau96I GGNCC 1 cut(s) 222
SetI ASST 5 cut(s) 227, 306, 340, 397, 505
Sfr303I CCGCGG 1 cut(s) 121
SgrBI CCGCGG 1 cut(s) 121
SinI GGWCC 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 201
SphI GCATGC 1 cut(s) 283
Sse9I AATT 4 cut(s) 89, 366, 398, 411
SsiI CCGC 3 cut(s) 72, 118, 120
SspMI CTAG 1 cut(s) 311
TasI AATT 4 cut(s) 89, 366, 398, 411
TatI WGTACW 1 cut(s) 535
TfiI GAWTC 1 cut(s) 425
Tru1I TTAA 4 cut(s) 60, 323, 410, 495
Tru9I TTAA 4 cut(s) 60, 323, 410, 495
TscAI CASTG 1 cut(s) 360
TseFI GTSAC 1 cut(s) 272
TseI GCWGC 3 cut(s) 346, 392, 404
Tsp45I GTSAC 1 cut(s) 272
TspDTI ATGAA 1 cut(s) 191
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 1 cut(s) 360
VpaK11BI GGWCC 1 cut(s) 222
XapI RAATTY 1 cut(s) 366
XceI RCATGY 1 cut(s) 283
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.