FvH4_6g42910

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016901
Physical Location & Seq
Reverse (-)
33317936 .. 33318868
933 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42910.t1

Sequence Viewer

Length: 534 bp
ATGGCGTCTTCAGGACCCATCAAACTCATGTCCTTACCTTCACCACTGCTTCTAGTTCTCGTATTCATGCTCATATCTCTAGCCATGGCTCAAAGGCCTACCGAAACCATTACCGAGTTCTACTTGCAAGACTTGGCATCCGGTTCAAATGCCACCGTTCAACCTATCACCGGCATAAGAAACAGGCCTTTGTCCTTCACTTCATTCGGCACCATTTTCGCCCTTGACGACCCAATCACAGTAACCCCGGACAAGAGCTCAACCGAAATTGGTCGAGCTCAAGGCATTATGGTGGCCTCTTCACTGTCTGGCTCTAACGTCCATGTTTCATTGTCGTTTGCTTTTAGCAATGGTAGCAGCCTTGAGGTACAAGGCACCAGCCGTCAGTTCGAGAGGTACAAGGAGCTAGCTGTGGTTTCAGGGACCGGAACTTTCAGATATGCGAGGGGTTATGCTACCTTGGAGAGCATTTACTATGACAACAGAACTTCCTACTCGATCATTCGCTGCACCACTAGGATCATTCTCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

178

Amino Acids

19.21

Weight (kDa)

8.82

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 39 - 173 8.9e-37 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016901)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g42910
prunus_persica Prupe.3G215200_v2.0.a1
pyrus_communis pycom17g09610
rosa_chinensis RchiOBHm_Chr2g0158831
rosa_laevigata RLG00000021117
rosa_multiflora Rmu_sc0002267.1_g000017 Rmu_sc0014404.1_g000001
rosa_roxburghii Rroxscaffold_2G00090960
rosa_rugosa Rorug02G0473000
rosa_samantha Rh2AG538800 Rh2BG551400 Rh2CG522100 Rh2DG561200
rosa_wichuraiana Rw2G044590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 209, 374
AclWI GGATC 1 cut(s) 527
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 369, 398
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 2 cut(s) 147, 161
AluBI AGCT 4 cut(s) 258, 278, 406, 410
AluI AGCT 4 cut(s) 258, 278, 406, 410
Alw21I GWGCWC 2 cut(s) 260, 280
AlwI GGATC 1 cut(s) 527
AoxI GGCC 3 cut(s) 95, 185, 294
ApeKI GCWGC 2 cut(s) 357, 507
AspS9I GGNCC 2 cut(s) 14, 423
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 33, 160
AsuNHI GCTAGC 1 cut(s) 406
AvaII GGWCC 2 cut(s) 14, 423
BanI GGYRCC 2 cut(s) 209, 374
BanII GRGCYC 2 cut(s) 260, 280
Bbv12I GWGCWC 2 cut(s) 260, 280
BbvI GCAGC 2 cut(s) 369, 494
BccI CCATC 1 cut(s) 26
BceAI ACGGC 1 cut(s) 366
BcgI CGANNNNNNTGC 2 cut(s) 199, 233
BcnI CCSGG 1 cut(s) 248
BfaI CTAG 4 cut(s) 53, 80, 407, 516
BisI GCNGC 2 cut(s) 358, 508
BlsI GCNGC 2 cut(s) 359, 509
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 2 cut(s) 14, 423
BmgT120I GGNCC 2 cut(s) 14, 423
BmiI GGNNCC 4 cut(s) 16, 211, 376, 424
BmrFI CCNGG 1 cut(s) 248
BmsI GCATC 1 cut(s) 146
BmtI GCTAGC 1 cut(s) 410
BpuEI CTTGAG 2 cut(s) 264, 383
BpuMI CCSGG 1 cut(s) 248
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 84, 246, 459
BsaWI WCCGGW 2 cut(s) 140, 425
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 355
BseDI CCNNGG 3 cut(s) 84, 246, 459
BseGI GGATG 1 cut(s) 137
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 1 cut(s) 355
BseXI GCAGC 2 cut(s) 369, 494
BsgI GTGCAG 1 cut(s) 493
BshFI GGCC 3 cut(s) 97, 187, 296
BshNI GGYRCC 2 cut(s) 209, 374
BsiHKAI GWGCWC 2 cut(s) 260, 280
BsiSI CCGG 4 cut(s) 141, 171, 248, 426
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 3 cut(s) 97, 187, 296
Bsp1286I GDGCHC 2 cut(s) 260, 280
Bsp143I GATC 2 cut(s) 498, 519
Bsp19I CCATGG 1 cut(s) 84
BspANI GGCC 3 cut(s) 97, 187, 296
BspLI GGNNCC 4 cut(s) 16, 211, 376, 424
BspOI GCTAGC 1 cut(s) 410
BspPI GGATC 1 cut(s) 527
BspT107I GGYRCC 2 cut(s) 209, 374
BsrDI GCAATG 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 3 cut(s) 84, 246, 459
BssMI GATC 2 cut(s) 498, 519
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 84, 459
Bst4CI ACNGT 3 cut(s) 157, 241, 306
Bst6I CTCTTC 1 cut(s) 304
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 408
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 1 cut(s) 137
BstKTI GATC 2 cut(s) 501, 522
BstMBI GATC 2 cut(s) 498, 519
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstSCI CCNGG 1 cut(s) 246
BstV1I GCAGC 2 cut(s) 369, 494
BsuRI GGCC 3 cut(s) 97, 187, 296
BtgI CCRYGG 1 cut(s) 84
BtsCI GGATG 1 cut(s) 137
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 2 cut(s) 44, 302
Cac8I GCNNGC 1 cut(s) 408
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 2 cut(s) 14, 423
Csp6I GTAC 2 cut(s) 368, 397
CviAII CATG 4 cut(s) 28, 67, 85, 323
CviQI GTAC 2 cut(s) 368, 397
DpnI GATC 2 cut(s) 500, 521
DpnII GATC 2 cut(s) 498, 519
Eam1104I CTCTTC 1 cut(s) 304
EarI CTCTTC 1 cut(s) 304
Ecl136II GAGCTC 2 cut(s) 258, 278
Eco130I CCWWGG 2 cut(s) 84, 459
Eco147I AGGCCT 2 cut(s) 97, 187
Eco24I GRGCYC 2 cut(s) 260, 280
Eco47I GGWCC 2 cut(s) 14, 423
Eco53kI GAGCTC 2 cut(s) 258, 278
EcoICRI GAGCTC 2 cut(s) 258, 278
EcoO109I RGGNCCY 1 cut(s) 14
EcoT14I CCWWGG 2 cut(s) 84, 459
EcoT38I GRGCYC 2 cut(s) 260, 280
ErhI CCWWGG 2 cut(s) 84, 459
FaeI CATG 4 cut(s) 31, 70, 88, 326
FaqI GGGAC 1 cut(s) 436
FatI CATG 4 cut(s) 27, 66, 84, 322
Fnu4HI GCNGC 2 cut(s) 358, 508
FokI GGATG 1 cut(s) 124
FriOI GRGCYC 2 cut(s) 260, 280
Fsp4HI GCNGC 2 cut(s) 358, 508
FspBI CTAG 4 cut(s) 53, 80, 407, 516
GluI GCNGC 2 cut(s) 358, 508
HaeIII GGCC 3 cut(s) 97, 187, 296
HapII CCGG 4 cut(s) 141, 171, 248, 426
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 4 cut(s) 31, 70, 88, 326
HpaII CCGG 4 cut(s) 141, 171, 248, 426
HphI GGTGA 2 cut(s) 33, 160
Hpy188I TCNGA 1 cut(s) 437
Hpy188III TCNNGA 3 cut(s) 12, 391, 531
HpyAV CCTTC 2 cut(s) 48, 205
HpyCH4III ACNGT 3 cut(s) 157, 241, 306
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 2 cut(s) 127, 510
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
HpySE526I ACGT 1 cut(s) 318
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 4 cut(s) 31, 70, 88, 326
Kzo9I GATC 2 cut(s) 498, 519
LmnI GCTCC 1 cut(s) 403
LpnPI CCDG 8 cut(s) 154, 169, 184, 261, 294, 391, 405, 439
Lsp1109I GCAGC 2 cut(s) 369, 494
LweI GCATC 1 cut(s) 146
MaeI CTAG 4 cut(s) 53, 80, 407, 516
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 1 cut(s) 241
MalI GATC 2 cut(s) 500, 521
MboI GATC 2 cut(s) 498, 519
MboII GAAGA 1 cut(s) 291
MhlI GDGCHC 2 cut(s) 260, 280
MluCI AATT 1 cut(s) 267
MnlI CCTC 4 cut(s) 307, 358, 387, 438
MslI CAYNNNNRTG 1 cut(s) 290
MspI CCGG 4 cut(s) 141, 171, 248, 426
MspR9I CCNGG 1 cut(s) 248
MwoI GCNNNNNNNGC 1 cut(s) 354
NciI CCSGG 1 cut(s) 248
NcoI CCATGG 1 cut(s) 84
NdeII GATC 2 cut(s) 498, 519
NheI GCTAGC 1 cut(s) 406
NlaIII CATG 4 cut(s) 31, 70, 88, 326
NlaIV GGNNCC 4 cut(s) 16, 211, 376, 424
PceI AGGCCT 2 cut(s) 97, 187
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PkrI GCNGC 2 cut(s) 359, 509
PpuMI RGGWCCY 1 cut(s) 14
Psp124BI GAGCTC 2 cut(s) 260, 280
Psp5II RGGWCCY 1 cut(s) 14
PspN4I GGNNCC 4 cut(s) 16, 211, 376, 424
PspPI GGNCC 2 cut(s) 14, 423
PspPPI RGGWCCY 1 cut(s) 14
RsaI GTAC 2 cut(s) 369, 398
RsaNI GTAC 2 cut(s) 368, 397
RseI CAYNNNNRTG 1 cut(s) 290
SacI GAGCTC 2 cut(s) 260, 280
SatI GCNGC 2 cut(s) 358, 508
Sau3AI GATC 2 cut(s) 498, 519
Sau96I GGNCC 2 cut(s) 14, 423
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 2 cut(s) 260, 280
SfaNI GCATC 1 cut(s) 146
SinI GGWCC 2 cut(s) 14, 423
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 2 cut(s) 279, 362
SmoI CTYRAG 2 cut(s) 279, 362
Sse9I AATT 1 cut(s) 267
SseBI AGGCCT 2 cut(s) 97, 187
SspMI CTAG 4 cut(s) 53, 80, 407, 516
SstI GAGCTC 2 cut(s) 260, 280
StuI AGGCCT 2 cut(s) 97, 187
StyD4I CCNGG 1 cut(s) 246
StyI CCWWGG 2 cut(s) 84, 459
TaaI ACNGT 3 cut(s) 157, 241, 306
TaiI ACGT 1 cut(s) 321
TaqI TCGA 3 cut(s) 274, 390, 497
TasI AATT 1 cut(s) 267
TscAI CASTG 2 cut(s) 51, 309
TseI GCWGC 2 cut(s) 357, 507
TspDTI ATGAA 3 cut(s) 55, 192, 318
TspRI CASTG 2 cut(s) 51, 309
VpaK11BI GGWCC 2 cut(s) 14, 423
XspI CTAG 4 cut(s) 53, 80, 407, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.