pycom17g09610

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0016901
Physical Location & Seq
Reverse (-)
7312414 .. 7312965
552 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g09610.1

Sequence Viewer

Length: 552 bp
ATGGCATTAAGTTCCCTTTCAAGACCCTCCAAACTCATGTCGGTTTTGTCAACATTGTTTCTAGCCATGCTAGTATCTCTTGCCAAAGCTCAAAGTCCTAGAGAAACCACCACAGTGTTTTACTTGCAAGACTTGGCGTCAGGACCCAATGCCACTGTTGTCCCTATCACAGGCATCCAAGGCAAGCCTCAGTCTTTCACCTCATTCGGCACCATTTTCGCCATTGACGATCCCATCACCGAAACTCCCGATAAGAGATCGACCCAAGTCGGTCGAGCTCAAGGCATTCTAGTGGCCTCGTCCTTGTCTGGCTCCAATGTGCATGTGTCAATGTCACTTGCATTCACCAATGGGCAGTACGATGGCAGCAGCCTTGAGCTACAAGGCACCAGCCGCCAGTTTGAGAGGGTTAAAGAGGTCTCAGTGGTTTCAGGCACTGCATCATTCAGATATGCAAGGGGTTATGCTACCCTTGAGACAGTTTTTTATGATAATAAGACCTCCTACTCAGTCATCCGGTGCACCATTAGGTTGCTTCTCAATAGTACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

184

Amino Acids

19.71

Weight (kDa)

9.37

Isoelectric Point (pI)

45.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 36 - 178 4.8e-43 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016901)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g42910
prunus_persica Prupe.3G215200_v2.0.a1
pyrus_communis pycom17g09610
rosa_chinensis RchiOBHm_Chr2g0158831
rosa_laevigata RLG00000021117
rosa_multiflora Rmu_sc0002267.1_g000017 Rmu_sc0014404.1_g000001
rosa_roxburghii Rroxscaffold_2G00090960
rosa_rugosa Rorug02G0473000
rosa_samantha Rh2AG538800 Rh2BG551400 Rh2CG522100 Rh2DG561200
rosa_wichuraiana Rw2G044590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 209, 386
AciI CCGC 1 cut(s) 394
AclWI GGATC 1 cut(s) 224
AcyI GRCGYC 1 cut(s) 137
AfaI GTAC 2 cut(s) 359, 547
AfiI CCNNNNNNNGG 1 cut(s) 170
AgsI TTSAA 1 cut(s) 21
AhdI GACNNNNNGTC 1 cut(s) 136
AleI CACNNNNGTG 1 cut(s) 113
AluBI AGCT 3 cut(s) 89, 278, 379
AluI AGCT 3 cut(s) 89, 278, 379
Alw21I GWGCWC 2 cut(s) 280, 524
Alw26I GTCTC 2 cut(s) 424, 470
Alw44I GTGCAC 1 cut(s) 520
AlwI GGATC 1 cut(s) 224
AlwNI CAGNNNCTG 1 cut(s) 437
AoxI GGCC 1 cut(s) 294
ApaLI GTGCAC 1 cut(s) 520
ApeKI GCWGC 2 cut(s) 366, 369
AspS9I GGNCC 1 cut(s) 143
AsuHPI GGTGA 3 cut(s) 190, 229, 337
AvaII GGWCC 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 524
BanI GGYRCC 2 cut(s) 209, 386
BanII GRGCYC 1 cut(s) 280
Bbv12I GWGCWC 2 cut(s) 280, 524
BbvI GCAGC 2 cut(s) 378, 381
BccI CCATC 2 cut(s) 242, 356
BcgI CGANNNNNNTGC 2 cut(s) 199, 233
BcoDI GTCTC 2 cut(s) 424, 470
BfaI CTAG 4 cut(s) 62, 71, 99, 290
BisI GCNGC 3 cut(s) 367, 370, 394
BlsI GCNGC 3 cut(s) 368, 371, 395
BmcAI AGTACT 1 cut(s) 547
Bme18I GGWCC 1 cut(s) 143
BmeRI GACNNNNNGTC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 4 cut(s) 145, 211, 313, 388
BmsI GCATC 2 cut(s) 183, 449
BoxI GACNNNNGTC 1 cut(s) 266
BpuEI CTTGAG 3 cut(s) 264, 395, 494
BsaHI GRCGYC 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 424
BsaJI CCNNGG 1 cut(s) 178
BsaWI WCCGGW 1 cut(s) 516
BsaXI ACNNNNNCTCC 2 cut(s) 229, 259
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse1I ACTGG 1 cut(s) 397
BseDI CCNNGG 1 cut(s) 178
BseGI GGATG 2 cut(s) 174, 513
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMII CTCAG 3 cut(s) 203, 435, 522
BseNI ACTGG 1 cut(s) 397
BseSI GKGCMC 1 cut(s) 524
BseXI GCAGC 2 cut(s) 378, 381
Bsh1285I CGRYCG 1 cut(s) 274
BshFI GGCC 1 cut(s) 296
BshNI GGYRCC 2 cut(s) 209, 386
BsiEI CGRYCG 1 cut(s) 274
BsiHKAI GWGCWC 2 cut(s) 280, 524
BsiSI CCGG 1 cut(s) 517
BslFI GGGAC 1 cut(s) 146
BslI CCNNNNNNNGG 1 cut(s) 170
BsmAI GTCTC 2 cut(s) 424, 470
BsmFI GGGAC 1 cut(s) 146
BsmI GAATGC 2 cut(s) 285, 341
BsnI GGCC 1 cut(s) 296
Bso31I GGTCTC 1 cut(s) 424
Bsp1286I GDGCHC 2 cut(s) 280, 524
Bsp143I GATC 2 cut(s) 229, 257
BspACI CCGC 1 cut(s) 394
BspANI GGCC 1 cut(s) 296
BspCNI CTCAG 3 cut(s) 202, 434, 521
BspLI GGNNCC 4 cut(s) 145, 211, 313, 388
BspPI GGATC 1 cut(s) 224
BspT107I GGYRCC 2 cut(s) 209, 386
BspTNI GGTCTC 1 cut(s) 424
BsrI ACTGG 1 cut(s) 397
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 2 cut(s) 229, 257
BssNI GRCGYC 1 cut(s) 137
BssT1I CCWWGG 1 cut(s) 178
Bst4CI ACNGT 3 cut(s) 115, 157, 481
BstACI GRCGYC 1 cut(s) 137
BstC8I GCNNGC 1 cut(s) 185
BstDEI CTNAG 3 cut(s) 189, 421, 508
BstENI CCTNNNNNAGG 1 cut(s) 168
BstF5I GGATG 2 cut(s) 174, 513
BstKTI GATC 2 cut(s) 232, 260
BstMAI GTCTC 2 cut(s) 424, 470
BstMBI GATC 2 cut(s) 229, 257
BstMCI CGRYCG 1 cut(s) 274
BstMWI GCNNNNNNNGC 2 cut(s) 180, 393
BstNSI RCATGY 1 cut(s) 326
BstPAI GACNNNNGTC 1 cut(s) 266
BstSLI GKGCMC 1 cut(s) 524
BstV1I GCAGC 2 cut(s) 378, 381
BsuRI GGCC 1 cut(s) 296
BtsCI GGATG 2 cut(s) 174, 513
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 4 cut(s) 120, 153, 429, 435
Cac8I GCNNGC 1 cut(s) 185
CaiI CAGNNNCTG 1 cut(s) 437
Cfr13I GGNCC 1 cut(s) 143
CseI GACGC 1 cut(s) 126
Csp6I GTAC 2 cut(s) 358, 546
CviAII CATG 3 cut(s) 37, 67, 323
CviJI RGCY 9 cut(s) 65, 89, 187, 278, 296, 312, 372, 379, 393
CviKI_1 RGCY 9 cut(s) 65, 89, 187, 278, 296, 312, 372, 379, 393
CviQI GTAC 2 cut(s) 358, 546
DdeI CTNAG 3 cut(s) 189, 421, 508
DpnI GATC 2 cut(s) 231, 259
DpnII GATC 2 cut(s) 229, 257
DriI GACNNNNNGTC 1 cut(s) 136
Eam1105I GACNNNNNGTC 1 cut(s) 136
Ecl136II GAGCTC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 178
Eco24I GRGCYC 1 cut(s) 280
Eco31I GGTCTC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 143
Eco53kI GAGCTC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 278
EcoNI CCTNNNNNAGG 1 cut(s) 168
EcoO109I RGGNCCY 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 178
FaeI CATG 3 cut(s) 40, 70, 326
FaiI YATR 6 cut(s) 38, 68, 324, 453, 465, 489
FaqI GGGAC 1 cut(s) 146
FatI CATG 3 cut(s) 36, 66, 322
Fnu4HI GCNGC 3 cut(s) 367, 370, 394
FokI GGATG 2 cut(s) 161, 500
FriOI GRGCYC 1 cut(s) 280
Fsp4HI GCNGC 3 cut(s) 367, 370, 394
FspBI CTAG 4 cut(s) 62, 71, 99, 290
GluI GCNGC 3 cut(s) 367, 370, 394
HaeIII GGCC 1 cut(s) 296
HapII CCGG 1 cut(s) 517
HgaI GACGC 1 cut(s) 126
Hin1I GRCGYC 1 cut(s) 137
Hin1II CATG 3 cut(s) 40, 70, 326
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HpaII CCGG 1 cut(s) 517
HphI GGTGA 3 cut(s) 190, 229, 337
Hpy166II GTNNAC 2 cut(s) 51, 522
Hpy188I TCNGA 1 cut(s) 449
Hpy188III TCNNGA 3 cut(s) 21, 141, 248
Hpy8I GTNNAC 2 cut(s) 51, 522
HpyCH4III ACNGT 3 cut(s) 115, 157, 481
HpyCH4V TGCA 6 cut(s) 127, 322, 341, 440, 455, 522
HpyF10VI GCNNNNNNNGC 2 cut(s) 180, 393
HpyF3I CTNAG 3 cut(s) 189, 421, 508
Hsp92I GRCGYC 1 cut(s) 137
Hsp92II CATG 3 cut(s) 40, 70, 326
Kzo9I GATC 2 cut(s) 229, 257
LmnI GCTCC 1 cut(s) 317
LpnPI CCDG 7 cut(s) 126, 156, 294, 403, 410, 417, 530
Lsp1109I GCAGC 2 cut(s) 378, 381
LweI GCATC 2 cut(s) 183, 449
MaeI CTAG 4 cut(s) 62, 71, 99, 290
MaeIII GTNAC 1 cut(s) 333
MalI GATC 2 cut(s) 231, 259
MboI GATC 2 cut(s) 229, 257
MhlI GDGCHC 2 cut(s) 280, 524
MnlI CCTC 7 cut(s) 37, 198, 211, 307, 399, 409, 511
MseI TTAA 3 cut(s) 8, 411, 550
MslI CAYNNNNRTG 2 cut(s) 113, 290
MspI CCGG 1 cut(s) 517
Mva1269I GAATGC 2 cut(s) 285, 341
MwoI GCNNNNNNNGC 2 cut(s) 180, 393
NdeII GATC 2 cut(s) 229, 257
NlaIII CATG 3 cut(s) 40, 70, 326
NlaIV GGNNCC 4 cut(s) 145, 211, 313, 388
NmuCI GTSAC 1 cut(s) 333
NspI RCATGY 1 cut(s) 326
OliI CACNNNNGTG 1 cut(s) 113
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PctI GAATGC 2 cut(s) 285, 341
PkrI GCNGC 3 cut(s) 368, 371, 395
PpuMI RGGWCCY 1 cut(s) 143
PshAI GACNNNNGTC 1 cut(s) 266
Psp124BI GAGCTC 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 143
PspN4I GGNNCC 4 cut(s) 145, 211, 313, 388
PspPI GGNCC 1 cut(s) 143
PspPPI RGGWCCY 1 cut(s) 143
PstNI CAGNNNCTG 1 cut(s) 437
RsaI GTAC 2 cut(s) 359, 547
RsaNI GTAC 2 cut(s) 358, 546
RseI CAYNNNNRTG 2 cut(s) 113, 290
SacI GAGCTC 1 cut(s) 280
SaqAI TTAA 3 cut(s) 8, 411, 550
SatI GCNGC 3 cut(s) 367, 370, 394
Sau3AI GATC 2 cut(s) 229, 257
Sau96I GGNCC 1 cut(s) 143
ScaI AGTACT 1 cut(s) 547
SduI GDGCHC 2 cut(s) 280, 524
SetI ASST 7 cut(s) 91, 203, 280, 381, 420, 503, 533
SfaNI GCATC 2 cut(s) 183, 449
SinI GGWCC 1 cut(s) 143
SmiMI CAYNNNNRTG 2 cut(s) 113, 290
SmlI CTYRAG 3 cut(s) 279, 374, 473
SmoI CTYRAG 3 cut(s) 279, 374, 473
SsiI CCGC 1 cut(s) 394
SspMI CTAG 4 cut(s) 62, 71, 99, 290
SstI GAGCTC 1 cut(s) 280
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 3 cut(s) 115, 157, 481
TaqI TCGA 2 cut(s) 260, 274
TaqII GACCGA 1 cut(s) 260
TatI WGTACW 1 cut(s) 545
TauI GCSGC 1 cut(s) 396
Tru1I TTAA 3 cut(s) 8, 411, 550
Tru9I TTAA 3 cut(s) 8, 411, 550
TscAI CASTG 4 cut(s) 120, 160, 429, 442
TseFI GTSAC 1 cut(s) 333
TseI GCWGC 2 cut(s) 366, 369
Tsp45I GTSAC 1 cut(s) 333
TspRI CASTG 4 cut(s) 120, 160, 429, 442
VneI GTGCAC 1 cut(s) 520
VpaK11BI GGWCC 1 cut(s) 143
XagI CCTNNNNNAGG 1 cut(s) 168
XceI RCATGY 1 cut(s) 326
XspI CTAG 4 cut(s) 62, 71, 99, 290
ZrmI AGTACT 1 cut(s) 547
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.