Rroxscaffold_2G00090960

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016901
Physical Location & Seq
Forward (+)
12626460 .. 12626993
534 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00090960.1

Sequence Viewer

Length: 534 bp
ATGGCATCTTCAAGACGCGTCAAACTCATGTCCTTTCCTTCACCACTGCTTCTAGTTCTTGTACTCATGCTCACATCTCTAGCCAAAGCTCAAAGGCTTACCGAAACCATCACGGAGTTCTACTTGCAAGACTTAGCCTCCGGTTCTAACGCAACCGTTATCCCCATCACCGGCATTAAAAACAAGCCTTTGTCCTTCACCTCATTCGGCACCATTTTCGTTCTTGATGATCCAATCACAGTAACCCCGGACAAAAGCTCGACTGAAATTGGTCGAGCTCAAGGCATCATGGTGGCCTCGTCACTGTCCGGCTCTAATGTCCATGTTTCAATGTCGTTTGCTTTTAGCAATGGTAGCAGCCTTGAGCTACAAGGCACCAGCCGTCAGTTCGAGAGGTACAAGGAGCTCTCTGTGGTTTCAGGGACCGGAACCTTCAGATATGCGAGGGGCTATGCTACCTTGGAGAGCATTTACTATGACAATAGAACTTCCTACTCTATCATACGGTGCACCACTAGGATCATTCTGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

177

Amino Acids

19.36

Weight (kDa)

9.51

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 39 - 173 2.4e-38 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016901)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g42910
prunus_persica Prupe.3G215200_v2.0.a1
pyrus_communis pycom17g09610
rosa_chinensis RchiOBHm_Chr2g0158831
rosa_laevigata RLG00000021117
rosa_multiflora Rmu_sc0002267.1_g000017 Rmu_sc0014404.1_g000001
rosa_roxburghii Rroxscaffold_2G00090960
rosa_rugosa Rorug02G0473000
rosa_samantha Rh2AG538800 Rh2BG551400 Rh2CG522100 Rh2DG561200
rosa_wichuraiana Rw2G044590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 209, 374
AccII CGCG 1 cut(s) 18
AclWI GGATC 2 cut(s) 224, 527
AcuI CTGAAG 1 cut(s) 418
AfaI GTAC 2 cut(s) 63, 398
AfiI CCNNNNNNNGG 1 cut(s) 170
AflIII ACRYGT 1 cut(s) 16
AgsI TTSAA 2 cut(s) 12, 330
AluBI AGCT 5 cut(s) 89, 258, 278, 367, 406
AluI AGCT 5 cut(s) 89, 258, 278, 367, 406
Alw21I GWGCWC 3 cut(s) 280, 408, 512
Alw44I GTGCAC 1 cut(s) 508
AlwI GGATC 2 cut(s) 224, 527
AoxI GGCC 1 cut(s) 294
ApaLI GTGCAC 1 cut(s) 508
ApeKI GCWGC 1 cut(s) 357
AspS9I GGNCC 1 cut(s) 423
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 3 cut(s) 33, 160, 190
AvaII GGWCC 1 cut(s) 423
BaeGI GKGCMC 1 cut(s) 512
BanI GGYRCC 2 cut(s) 209, 374
BanII GRGCYC 2 cut(s) 280, 408
Bbv12I GWGCWC 3 cut(s) 280, 408, 512
BbvI GCAGC 1 cut(s) 369
BccI CCATC 2 cut(s) 116, 173
BceAI ACGGC 1 cut(s) 366
BcgI CGANNNNNNTGC 2 cut(s) 199, 233
BcnI CCSGG 1 cut(s) 248
BfaI CTAG 3 cut(s) 53, 80, 516
BisI GCNGC 1 cut(s) 358
BlsI GCNGC 1 cut(s) 359
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 423
BmgT120I GGNCC 1 cut(s) 423
BmiI GGNNCC 4 cut(s) 211, 376, 424, 430
BmrFI CCNGG 1 cut(s) 248
BmsI GCATC 2 cut(s) 14, 294
BpuEI CTTGAG 2 cut(s) 264, 383
BpuMI CCSGG 1 cut(s) 248
BsaJI CCNNGG 2 cut(s) 246, 459
BsaWI WCCGGW 2 cut(s) 140, 425
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 1 cut(s) 170
Bse118I RCCGGY 1 cut(s) 170
Bse3DI GCAATG 1 cut(s) 355
BseDI CCNNGG 2 cut(s) 246, 459
BseLI CCNNNNNNNGG 1 cut(s) 170
BseMI GCAATG 1 cut(s) 355
BseSI GKGCMC 1 cut(s) 512
BseXI GCAGC 1 cut(s) 369
Bsh1236I CGCG 1 cut(s) 18
BshFI GGCC 1 cut(s) 296
BshNI GGYRCC 2 cut(s) 209, 374
BsiHKAI GWGCWC 3 cut(s) 280, 408, 512
BsiSI CCGG 5 cut(s) 141, 171, 248, 309, 426
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 436
BsnI GGCC 1 cut(s) 296
Bsp1286I GDGCHC 3 cut(s) 280, 408, 512
Bsp143I GATC 2 cut(s) 229, 519
BspANI GGCC 1 cut(s) 296
BspFNI CGCG 1 cut(s) 18
BspLI GGNNCC 4 cut(s) 211, 376, 424, 430
BspPI GGATC 2 cut(s) 224, 527
BspT107I GGYRCC 2 cut(s) 209, 374
BsrDI GCAATG 1 cut(s) 355
BsrFI RCCGGY 1 cut(s) 170
BssAI RCCGGY 1 cut(s) 170
BssECI CCNNGG 2 cut(s) 246, 459
BssMI GATC 2 cut(s) 229, 519
BssT1I CCWWGG 1 cut(s) 459
Bst4CI ACNGT 4 cut(s) 157, 241, 306, 507
BstDEI CTNAG 1 cut(s) 133
BstFNI CGCG 1 cut(s) 18
BstKTI GATC 2 cut(s) 232, 522
BstMBI GATC 2 cut(s) 229, 519
BstMWI GCNNNNNNNGC 1 cut(s) 354
BstSCI CCNGG 1 cut(s) 246
BstSLI GKGCMC 1 cut(s) 512
BstUI CGCG 1 cut(s) 18
BstV1I GCAGC 1 cut(s) 369
BsuRI GGCC 1 cut(s) 296
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 2 cut(s) 44, 302
Cfr10I RCCGGY 1 cut(s) 170
Cfr13I GGNCC 1 cut(s) 423
CseI GACGC 2 cut(s) 7, 24
Csp6I GTAC 2 cut(s) 62, 397
CviAII CATG 4 cut(s) 28, 67, 289, 323
CviQI GTAC 2 cut(s) 62, 397
DdeI CTNAG 1 cut(s) 133
DpnI GATC 2 cut(s) 231, 521
DpnII GATC 2 cut(s) 229, 519
Ecl136II GAGCTC 2 cut(s) 278, 406
Eco130I CCWWGG 1 cut(s) 459
Eco24I GRGCYC 2 cut(s) 280, 408
Eco47I GGWCC 1 cut(s) 423
Eco53kI GAGCTC 2 cut(s) 278, 406
Eco57I CTGAAG 1 cut(s) 418
EcoICRI GAGCTC 2 cut(s) 278, 406
EcoT14I CCWWGG 1 cut(s) 459
EcoT38I GRGCYC 2 cut(s) 280, 408
ErhI CCWWGG 1 cut(s) 459
FaeI CATG 4 cut(s) 31, 70, 292, 326
FaiI YATR 8 cut(s) 29, 68, 290, 324, 441, 453, 477, 503
FaqI GGGAC 1 cut(s) 436
FatI CATG 4 cut(s) 27, 66, 288, 322
Fnu4HI GCNGC 1 cut(s) 358
FriOI GRGCYC 2 cut(s) 280, 408
Fsp4HI GCNGC 1 cut(s) 358
FspBI CTAG 3 cut(s) 53, 80, 516
GluI GCNGC 1 cut(s) 358
HaeIII GGCC 1 cut(s) 296
HapII CCGG 5 cut(s) 141, 171, 248, 309, 426
HgaI GACGC 2 cut(s) 7, 24
Hin1II CATG 4 cut(s) 31, 70, 292, 326
HpaII CCGG 5 cut(s) 141, 171, 248, 309, 426
HphI GGTGA 3 cut(s) 33, 160, 190
Hpy166II GTNNAC 1 cut(s) 510
Hpy188I TCNGA 1 cut(s) 437
Hpy188III TCNNGA 4 cut(s) 12, 224, 391, 531
Hpy8I GTNNAC 1 cut(s) 510
HpyAV CCTTC 3 cut(s) 48, 205, 442
HpyCH4III ACNGT 4 cut(s) 157, 241, 306, 507
HpyCH4V TGCA 2 cut(s) 127, 510
HpyF10VI GCNNNNNNNGC 1 cut(s) 354
HpyF3I CTNAG 1 cut(s) 133
Hsp92II CATG 4 cut(s) 31, 70, 292, 326
Kzo9I GATC 2 cut(s) 229, 519
LmnI GCTCC 1 cut(s) 403
LpnPI CCDG 7 cut(s) 154, 184, 261, 322, 391, 405, 439
Lsp1109I GCAGC 1 cut(s) 369
LweI GCATC 2 cut(s) 14, 294
MaeI CTAG 3 cut(s) 53, 80, 516
MaeIII GTNAC 2 cut(s) 241, 300
MalI GATC 2 cut(s) 231, 521
MboI GATC 2 cut(s) 229, 519
MhlI GDGCHC 3 cut(s) 280, 408, 512
MluCI AATT 1 cut(s) 267
MluI ACGCGT 1 cut(s) 16
MnlI CCTC 5 cut(s) 148, 211, 307, 387, 438
MseI TTAA 1 cut(s) 177
MslI CAYNNNNRTG 1 cut(s) 290
MspI CCGG 5 cut(s) 141, 171, 248, 309, 426
MspR9I CCNGG 1 cut(s) 248
MvnI CGCG 1 cut(s) 18
MwoI GCNNNNNNNGC 1 cut(s) 354
NciI CCSGG 1 cut(s) 248
NdeII GATC 2 cut(s) 229, 519
NlaIII CATG 4 cut(s) 31, 70, 292, 326
NlaIV GGNNCC 4 cut(s) 211, 376, 424, 430
NmuCI GTSAC 1 cut(s) 300
PkrI GCNGC 1 cut(s) 359
Psp124BI GAGCTC 2 cut(s) 280, 408
PspN4I GGNNCC 4 cut(s) 211, 376, 424, 430
PspPI GGNCC 1 cut(s) 423
RsaI GTAC 2 cut(s) 63, 398
RsaNI GTAC 2 cut(s) 62, 397
RseI CAYNNNNRTG 1 cut(s) 290
SacI GAGCTC 2 cut(s) 280, 408
SaqAI TTAA 1 cut(s) 177
SatI GCNGC 1 cut(s) 358
Sau3AI GATC 2 cut(s) 229, 519
Sau96I GGNCC 1 cut(s) 423
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 3 cut(s) 280, 408, 512
SetI ASST 9 cut(s) 91, 203, 260, 280, 369, 398, 408, 434, 461
SfaNI GCATC 2 cut(s) 14, 294
SinI GGWCC 1 cut(s) 423
SmiMI CAYNNNNRTG 1 cut(s) 290
SmlI CTYRAG 2 cut(s) 279, 362
SmoI CTYRAG 2 cut(s) 279, 362
Sse9I AATT 1 cut(s) 267
SspMI CTAG 3 cut(s) 53, 80, 516
SstI GAGCTC 2 cut(s) 280, 408
StyD4I CCNGG 1 cut(s) 246
StyI CCWWGG 1 cut(s) 459
TaaI ACNGT 4 cut(s) 157, 241, 306, 507
TaqI TCGA 3 cut(s) 260, 274, 390
TasI AATT 1 cut(s) 267
TatI WGTACW 1 cut(s) 61
Tru1I TTAA 1 cut(s) 177
Tru9I TTAA 1 cut(s) 177
TscAI CASTG 2 cut(s) 51, 309
TseFI GTSAC 1 cut(s) 300
TseI GCWGC 1 cut(s) 357
Tsp45I GTSAC 1 cut(s) 300
TspGWI ACGGA 1 cut(s) 128
TspRI CASTG 2 cut(s) 51, 309
VneI GTGCAC 1 cut(s) 508
VpaK11BI GGWCC 1 cut(s) 423
XspI CTAG 3 cut(s) 53, 80, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.