RLG00000021117

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016901
Physical Location & Seq
Reverse (-)
72107593 .. 72109024
1432 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021117

Sequence Viewer

Length: 810 bp
ATGCCAGTTGCAAGCATCGCCGGAAAGCTGTGGACCTTCACTCAATTCGGAACAATTTATGTCACCGATGACACCATGACCCAAGGACCGAACATAACATCTCCCATAGTGGGACGGGCCCAAGGCATAACGGTGGCATCGGCACTGGACGGAAGCAATGCCCTTGTCTTGGTGTCTCTTGTGTTCACAAACATGGAGTACAATGGCAGCACTCTAGAGATTCAAGGAAATAGTAAGCAATTCGAGTCGGTCAGAGAGGTTGCAGTTGTATCTGGCACCGGAAAGTTTCGACCCGTCAAACTCATGTCCTTACCTTCACCACTGCTTCTAGTTCTTGTACTCATGCTCACATCTCTAGCCAAGGCTCAAAGGCTTAGCGAAACCATCACGGAGTTCTACTTGCAAGACTTAGCCTCCGGTTCTAACGCCACCGTTATCCCCATCACCGGCATTAAAAACAAGCCTTTGTCCTTCACCTCATTCGGCACCATTTTCGCTCTTGATGATCCAATCACAGTAACCCCGGACAAAAGCTCGACTGAAATTGGTCGAGCTCAAGGCATCATGGTGGCCTCGTCACTGTCCGGCTCTAATGTCCATGTTTCAATGTCGTTTGCTTTTAGCAATGGTAGCAGCCTTGAGATACAAGGCACCAGTCGTCAGTTCGAGAGGTACAAGGAGCTCTCTGTGGTTTCCGGGACCGGAACCTTCAGATATGCTAGGGGCTATGCTACCTTGGAGAGCATTTACTATGACAATAGAACTTCCTACTCTATCATACGGTGCACCACTAGGATCATTCTGTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

270

Amino Acids

28.97

Weight (kDa)

8.62

Isoelectric Point (pI)

30.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 8 - 97 2.2e-29 Dirigent-like protein
Dirigent PF03018 131 - 264 2e-37 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016901)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g42910
prunus_persica Prupe.3G215200_v2.0.a1
pyrus_communis pycom17g09610
rosa_chinensis RchiOBHm_Chr2g0158831
rosa_laevigata RLG00000021117
rosa_multiflora Rmu_sc0002267.1_g000017 Rmu_sc0014404.1_g000001
rosa_roxburghii Rroxscaffold_2G00090960
rosa_rugosa Rorug02G0473000
rosa_samantha Rh2AG538800 Rh2BG551400 Rh2CG522100 Rh2DG561200
rosa_wichuraiana Rw2G044590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 275, 485, 650
AclWI GGATC 2 cut(s) 500, 803
AcuI CTGAAG 1 cut(s) 694
AfaI GTAC 3 cut(s) 200, 339, 674
AfiI CCNNNNNNNGG 3 cut(s) 110, 169, 446
AgsI TTSAA 2 cut(s) 224, 606
AluBI AGCT 4 cut(s) 28, 534, 554, 682
AluI AGCT 4 cut(s) 28, 534, 554, 682
Alw21I GWGCWC 3 cut(s) 556, 684, 788
Alw26I GTCTC 1 cut(s) 180
Alw44I GTGCAC 1 cut(s) 784
AlwI GGATC 2 cut(s) 500, 803
AoxI GGCC 2 cut(s) 117, 570
ApaI GGGCCC 1 cut(s) 121
ApaLI GTGCAC 1 cut(s) 784
ApeKI GCWGC 2 cut(s) 207, 633
AspS9I GGNCC 5 cut(s) 33, 86, 117, 118, 699
AsuC2I CCSGG 2 cut(s) 524, 697
AsuHPI GGTGA 4 cut(s) 55, 309, 436, 466
AvaII GGWCC 3 cut(s) 33, 86, 699
BaeGI GKGCMC 2 cut(s) 121, 788
BanI GGYRCC 3 cut(s) 275, 485, 650
BanII GRGCYC 3 cut(s) 121, 556, 684
Bbv12I GWGCWC 3 cut(s) 556, 684, 788
BbvI GCAGC 2 cut(s) 219, 645
BccI CCATC 2 cut(s) 392, 449
BcgI CGANNNNNNTGC 2 cut(s) 475, 509
BcnI CCSGG 2 cut(s) 524, 697
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 5 cut(s) 215, 329, 356, 720, 792
BisI GCNGC 2 cut(s) 208, 634
BlpI GCTNAGC 1 cut(s) 374
BlsI GCNGC 2 cut(s) 209, 635
Bme1390I CCNGG 2 cut(s) 524, 697
Bme18I GGWCC 3 cut(s) 33, 86, 699
BmgT120I GGNCC 5 cut(s) 33, 86, 117, 118, 699
BmiI GGNNCC 6 cut(s) 119, 277, 487, 652, 700, 706
BmrFI CCNGG 2 cut(s) 524, 697
BmsI GCATC 3 cut(s) 24, 146, 570
Bpu1102I GCTNAGC 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 540, 659
BpuMI CCSGG 2 cut(s) 524, 697
BsaJI CCNNGG 5 cut(s) 82, 121, 360, 522, 735
BsaWI WCCGGW 3 cut(s) 278, 416, 701
BsaXI ACNNNNNCTCC 2 cut(s) 398, 428
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 169, 446
Bse118I RCCGGY 1 cut(s) 446
Bse1I ACTGG 3 cut(s) 5, 150, 654
Bse3DI GCAATG 2 cut(s) 163, 631
BseDI CCNNGG 5 cut(s) 82, 121, 360, 522, 735
BseLI CCNNNNNNNGG 3 cut(s) 110, 169, 446
BseMI GCAATG 2 cut(s) 163, 631
BseNI ACTGG 3 cut(s) 5, 150, 654
BseSI GKGCMC 2 cut(s) 121, 788
BseXI GCAGC 2 cut(s) 219, 645
BshFI GGCC 2 cut(s) 119, 572
BshNI GGYRCC 3 cut(s) 275, 485, 650
BsiHKAI GWGCWC 3 cut(s) 556, 684, 788
BsiSI CCGG 8 cut(s) 21, 279, 417, 447, 524, 585, 696, 702
BslFI GGGAC 2 cut(s) 126, 712
BslI CCNNNNNNNGG 3 cut(s) 110, 169, 446
BsmAI GTCTC 1 cut(s) 180
BsmFI GGGAC 2 cut(s) 126, 712
BsnI GGCC 2 cut(s) 119, 572
Bsp120I GGGCCC 1 cut(s) 117
Bsp1286I GDGCHC 4 cut(s) 121, 556, 684, 788
Bsp143I GATC 2 cut(s) 505, 795
Bsp1720I GCTNAGC 1 cut(s) 374
BspANI GGCC 2 cut(s) 119, 572
BspLI GGNNCC 6 cut(s) 119, 277, 487, 652, 700, 706
BspPI GGATC 2 cut(s) 500, 803
BspT107I GGYRCC 3 cut(s) 275, 485, 650
BsrDI GCAATG 2 cut(s) 163, 631
BsrFI RCCGGY 1 cut(s) 446
BsrI ACTGG 3 cut(s) 5, 150, 654
BssAI RCCGGY 1 cut(s) 446
BssECI CCNNGG 5 cut(s) 82, 121, 360, 522, 735
BssMI GATC 2 cut(s) 505, 795
BssT1I CCWWGG 4 cut(s) 82, 121, 360, 735
Bst4CI ACNGT 5 cut(s) 133, 433, 517, 582, 783
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 2 cut(s) 374, 409
BstKTI GATC 2 cut(s) 508, 798
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 2 cut(s) 505, 795
BstMWI GCNNNNNNNGC 2 cut(s) 17, 630
BstSCI CCNGG 2 cut(s) 522, 695
BstSLI GKGCMC 2 cut(s) 121, 788
BstV1I GCAGC 2 cut(s) 219, 645
BsuRI GGCC 2 cut(s) 119, 572
BtsI GCAGTG 1 cut(s) 320
BtsIMutI CAGTG 3 cut(s) 143, 320, 578
Cac8I GCNNGC 1 cut(s) 13
Cfr10I RCCGGY 1 cut(s) 446
Cfr13I GGNCC 5 cut(s) 33, 86, 117, 118, 699
Csp6I GTAC 3 cut(s) 199, 338, 673
CviAII CATG 6 cut(s) 76, 193, 304, 343, 565, 599
CviQI GTAC 3 cut(s) 199, 338, 673
DdeI CTNAG 2 cut(s) 374, 409
DpnI GATC 2 cut(s) 507, 797
DpnII GATC 2 cut(s) 505, 795
Ecl136II GAGCTC 2 cut(s) 554, 682
Eco130I CCWWGG 4 cut(s) 82, 121, 360, 735
Eco24I GRGCYC 3 cut(s) 121, 556, 684
Eco47I GGWCC 3 cut(s) 33, 86, 699
Eco53kI GAGCTC 2 cut(s) 554, 682
Eco57I CTGAAG 1 cut(s) 694
EcoICRI GAGCTC 2 cut(s) 554, 682
EcoT14I CCWWGG 4 cut(s) 82, 121, 360, 735
EcoT38I GRGCYC 3 cut(s) 121, 556, 684
ErhI CCWWGG 4 cut(s) 82, 121, 360, 735
FaeI CATG 6 cut(s) 79, 196, 307, 346, 568, 602
FaqI GGGAC 2 cut(s) 126, 712
FatI CATG 6 cut(s) 75, 192, 303, 342, 564, 598
Fnu4HI GCNGC 2 cut(s) 208, 634
FriOI GRGCYC 3 cut(s) 121, 556, 684
Fsp4HI GCNGC 2 cut(s) 208, 634
FspBI CTAG 5 cut(s) 215, 329, 356, 720, 792
GluI GCNGC 2 cut(s) 208, 634
HaeIII GGCC 2 cut(s) 119, 572
HapII CCGG 8 cut(s) 21, 279, 417, 447, 524, 585, 696, 702
Hin1II CATG 6 cut(s) 79, 196, 307, 346, 568, 602
HinfI GANTC 2 cut(s) 220, 245
HpaII CCGG 8 cut(s) 21, 279, 417, 447, 524, 585, 696, 702
HphI GGTGA 4 cut(s) 55, 309, 436, 466
Hpy166II GTNNAC 3 cut(s) 33, 186, 786
Hpy188I TCNGA 3 cut(s) 50, 254, 713
Hpy188III TCNNGA 4 cut(s) 215, 500, 667, 807
Hpy8I GTNNAC 3 cut(s) 33, 186, 786
HpyAV CCTTC 4 cut(s) 46, 324, 481, 718
HpyCH4III ACNGT 5 cut(s) 133, 433, 517, 582, 783
HpyCH4V TGCA 4 cut(s) 11, 263, 403, 786
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 630
HpyF3I CTNAG 2 cut(s) 374, 409
Hsp92II CATG 6 cut(s) 79, 196, 307, 346, 568, 602
Kzo9I GATC 2 cut(s) 505, 795
LmnI GCTCC 1 cut(s) 679
Lsp1109I GCAGC 2 cut(s) 219, 645
LweI GCATC 3 cut(s) 24, 146, 570
MaeI CTAG 5 cut(s) 215, 329, 356, 720, 792
MaeIII GTNAC 3 cut(s) 61, 517, 576
MalI GATC 2 cut(s) 507, 797
MboI GATC 2 cut(s) 505, 795
MhlI GDGCHC 4 cut(s) 121, 556, 684, 788
MluCI AATT 4 cut(s) 44, 54, 239, 543
MlyI GAGTC 1 cut(s) 254
MnlI CCTC 5 cut(s) 250, 424, 487, 583, 663
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 3 cut(s) 131, 191, 566
MspI CCGG 8 cut(s) 21, 279, 417, 447, 524, 585, 696, 702
MspR9I CCNGG 2 cut(s) 524, 697
MwoI GCNNNNNNNGC 2 cut(s) 17, 630
NciI CCSGG 2 cut(s) 524, 697
NdeII GATC 2 cut(s) 505, 795
NlaIII CATG 6 cut(s) 79, 196, 307, 346, 568, 602
NlaIV GGNNCC 6 cut(s) 119, 277, 487, 652, 700, 706
NmuCI GTSAC 2 cut(s) 61, 576
PfeI GAWTC 1 cut(s) 220
PfoI TCCNGGA 1 cut(s) 695
PkrI GCNGC 2 cut(s) 209, 635
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
Psp124BI GAGCTC 2 cut(s) 556, 684
PspN4I GGNNCC 6 cut(s) 119, 277, 487, 652, 700, 706
PspOMI GGGCCC 1 cut(s) 117
PspPI GGNCC 5 cut(s) 33, 86, 117, 118, 699
RsaI GTAC 3 cut(s) 200, 339, 674
RsaNI GTAC 3 cut(s) 199, 338, 673
RseI CAYNNNNRTG 3 cut(s) 131, 191, 566
SacI GAGCTC 2 cut(s) 556, 684
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 2 cut(s) 208, 634
Sau3AI GATC 2 cut(s) 505, 795
Sau96I GGNCC 5 cut(s) 33, 86, 117, 118, 699
SchI GAGTC 1 cut(s) 254
ScrFI CCNGG 2 cut(s) 524, 697
SduI GDGCHC 4 cut(s) 121, 556, 684, 788
SfaNI GCATC 3 cut(s) 24, 146, 570
SinI GGWCC 3 cut(s) 33, 86, 699
SmiMI CAYNNNNRTG 3 cut(s) 131, 191, 566
SmlI CTYRAG 2 cut(s) 555, 638
SmoI CTYRAG 2 cut(s) 555, 638
Sse9I AATT 4 cut(s) 44, 54, 239, 543
SspMI CTAG 5 cut(s) 215, 329, 356, 720, 792
SstI GAGCTC 2 cut(s) 556, 684
StyD4I CCNGG 2 cut(s) 522, 695
StyI CCWWGG 4 cut(s) 82, 121, 360, 735
TaaI ACNGT 5 cut(s) 133, 433, 517, 582, 783
TaqI TCGA 5 cut(s) 243, 289, 536, 550, 666
TaqII GACCGA 2 cut(s) 103, 238
TasI AATT 4 cut(s) 44, 54, 239, 543
TatI WGTACW 2 cut(s) 198, 337
TfiI GAWTC 1 cut(s) 220
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 3 cut(s) 150, 327, 585
TseFI GTSAC 2 cut(s) 61, 576
TseI GCWGC 2 cut(s) 207, 633
Tsp45I GTSAC 2 cut(s) 61, 576
TspGWI ACGGA 2 cut(s) 165, 404
TspRI CASTG 3 cut(s) 150, 327, 585
VneI GTGCAC 1 cut(s) 784
VpaK11BI GGWCC 3 cut(s) 33, 86, 699
XbaI TCTAGA 1 cut(s) 214
XspI CTAG 5 cut(s) 215, 329, 356, 720, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.