FvH4_6g42990

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013817
Physical Location & Seq
Reverse (-)
33349172 .. 33353616
4445 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g42990.t1

Sequence Viewer

Length: 1494 bp
ATGCAGGGAATATTGATAAACGGGCAATTCCCAGGGCCACAGATTGATTGTGTGACCAATGATAACCTTATTATCAGTGTTTTCAACAGCTTGGATGAACCTTTTCTCATTTCTTGGAATGGTGTGCAGCAGAGAAGGAACTCATGGCAAGATGGGGTCTTTGGCACAAATTGTCCCATTCCACCAGGACAGAACTTCACTTATGTTCTTCAAGTCAAAGATCAGATTGGAAGTTACTATTATTTTCCTTCCCTTGCATTCCACAAAGCTGCAGGAGGGTATGGTGGTATCAAGATCTCAAGTCGACCTAGAATTCCAGTGCCTTTTGATCCTCCTGCAGGAGATTTCACCATTCTGGCTGGAGACTGGTTCAAGCAAAATCACACTGATCTGAAGGCAATTTTAGATGGTGGGAATAATCTTCCCTTCCCAGACGGACTGATCATCAATGGCCGCGGATCAAATGGGAATACATTTACAGTTGATCAAGGCAAAACTTACAGGTTCAGAATATCAAATGTTGGAATTGCAACATCCATCAATTTCAGGATCCAAGGTCACAAGATGCTGTTGGTTGAGGTGGAAGGCATTCACACCTTGCAAAATACTTACAGCTCTCTTGACATTCATTTGGGACAGTCACTCTCTGTTCTGGTAACAGCTGATCAACCACCACAAGATTACTACATTGTCGTCTCTACTCGTTTCACCTCACAAGTGCTCTCCGCAACCTCCATTCTTCACTACAGTAACTCGGGAGGAAGTGTGTCTGGTCCTCCACCTGGTGGCCCAACTACTGAGGTCGACTGGTCTCTTGAACAAGCTCGATCTTTCAGGCGGAATTTGACAGCAAGCGGACCAAGGCCTAACCCTCAAGGCTCATATCATTATGGATTGATCAACACCACCAGGACAATCAGGCTTACAAATTCTGCTCCTATAATCAATGGCAAGCAAAGATATGCTATAAATAGCGTTTCCTTCCTCCAACCTGACACACCACTAAAAGTTGCTGACTACTTCAAGATCTCCGGAGTGTTCTCCGTTGGAAGCATCTCGGATAATCCCACTGGTGGCGGTGGTTATCTCCAGACTTCTGTCATGCATGCTGATTTCCGAGCCTTTGTTGAATTTGTGTTTGAGAATCCGGAGAATACTCTGCAGTCATGGCACCTTGATGGTTATAGTTTCTTTGTTGTTGGTATGGATGGTGGACAGTGGACACCAGCAAGCAGATTAAGATACAATTTGATGGACACTGTTTCTCGTTGCACCGTTCAGGTATATCCCAACTCATGGACTGCAATTTATGTGCCTTTGGACAATGTGGGAATGTGGAATGTGAGATCTGAGAATTGGGCTAGACAGTATTTAGGCCAGCAGTTCTATCTTCGTGTATACTCACCGGTAAATTCATGGAGGGATGAATATCCTATTCCGAAGAATGCTCTTCTGTGTGGTCGAGCATCAGGCCGGCGAACTCGGCCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

498

Amino Acids

55.19

Weight (kDa)

8.71

Isoelectric Point (pI)

38.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 2 - 102 4.3e-37 Multicopper oxidase
Cu-oxidase PF00394 115 - 252 2.8e-42 Multicopper oxidase
Cu-oxidase_2 PF07731 332 - 469 5.8e-32 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013817)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g42990 FvH4_6g42990
malus_domestica MD09G1110500.v1.1 MD17G1098300.v1.1
prunus_persica Prupe.3G216000_v2.0.a1
pyrus_communis pycom09g03290 pycom17g09370
rosa_chinensis RchiOBHm_Chr2g0158971
rosa_laevigata RLG00000021129
rosa_multiflora Rmu_sc0007907.1_g000005
rosa_roxburghii Rroxscaffold_2G00090830
rosa_rugosa Rorug02G0473300
rosa_samantha Rh2AG539900 Rh2BG552700 Rh2CG523200 Rh2DG562500
rosa_wichuraiana Rw2G044690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1170
AccB7I CCANNNNNTGG 2 cut(s) 785, 1296
AccI GTMKAC 3 cut(s) 304, 804, 1398
AccII CGCG 1 cut(s) 456
AccIII TCCGGA 2 cut(s) 1031, 1147
AciI CCGC 6 cut(s) 454, 456, 726, 838, 855, 1077
AclWI GGATC 4 cut(s) 323, 466, 544, 557
AcoI YGGCCR 1 cut(s) 451
AcsI RAATTY 5 cut(s) 312, 841, 928, 1130, 1411
AcuI CTGAAG 1 cut(s) 413
AdeI CACNNNGTG 1 cut(s) 785
AfiI CCNNNNNNNGG 5 cut(s) 338, 782, 785, 1073, 1296
AgeI ACCGGT 1 cut(s) 1405
AgsI TTSAA 6 cut(s) 85, 212, 373, 818, 1024, 1130
AjnI CCWGG 4 cut(s) 31, 184, 781, 908
AluBI AGCT 5 cut(s) 90, 269, 615, 662, 824
AluI AGCT 5 cut(s) 90, 269, 615, 662, 824
Alw21I GWGCWC 1 cut(s) 723
Alw26I GTCTC 3 cut(s) 357, 700, 816
AlwI GGATC 4 cut(s) 323, 466, 544, 557
Ama87I CYCGRG 1 cut(s) 754
Aor13HI TCCGGA 2 cut(s) 1031, 1147
AoxI GGCC 7 cut(s) 35, 451, 787, 863, 1375, 1471, 1484
ApeKI GCWGC 2 cut(s) 127, 269
ApoI RAATTY 5 cut(s) 312, 841, 928, 1130, 1411
AsiGI ACCGGT 1 cut(s) 1405
Asp700I GAANNNNTTC 2 cut(s) 102, 588
AspS9I GGNCC 4 cut(s) 35, 773, 788, 857
AsuHPI GGTGA 3 cut(s) 340, 700, 1395
AvaI CYCGRG 1 cut(s) 754
AvaII GGWCC 2 cut(s) 773, 857
BamHI GGATCC 1 cut(s) 549
BanI GGYRCC 1 cut(s) 1170
Bbv12I GWGCWC 1 cut(s) 723
BbvI GCAGC 2 cut(s) 139, 256
BccI CCATC 6 cut(s) 146, 401, 545, 1172, 1202, 1246
BciT130I CCWGG 4 cut(s) 33, 186, 783, 910
BclI TGATCA 4 cut(s) 441, 484, 664, 897
BcoDI GTCTC 3 cut(s) 357, 700, 816
BfaI CTAG 2 cut(s) 309, 1362
BfmI CTRYAG 4 cut(s) 270, 336, 745, 1160
BglII AGATCT 3 cut(s) 294, 1026, 1346
BisI GCNGC 3 cut(s) 128, 270, 454
BlsI GCNGC 3 cut(s) 129, 271, 455
Bme1390I CCNGG 4 cut(s) 33, 186, 783, 910
Bme18I GGWCC 2 cut(s) 773, 857
BmeT110I CYCGRG 1 cut(s) 754
BmgT120I GGNCC 4 cut(s) 35, 773, 788, 857
BmiI GGNNCC 2 cut(s) 551, 1172
BmrFI CCNGG 4 cut(s) 33, 186, 783, 910
BmsI GCATC 3 cut(s) 555, 1062, 1475
BoxI GACNNNNGTC 1 cut(s) 1097
BpmI CTGGAG 2 cut(s) 381, 1073
BpuEI CTTGAG 2 cut(s) 283, 858
BsaBI GATNNNNATC 1 cut(s) 1428
BsaI GGTCTC 1 cut(s) 816
BsaJI CCNNGG 5 cut(s) 31, 32, 454, 553, 860
BsaWI WCCGGW 3 cut(s) 1031, 1147, 1405
BsaXI ACNNNNNCTCC 2 cut(s) 750, 780
Bsc4I CCNNNNNNNGG 5 cut(s) 338, 782, 785, 1073, 1296
Bse118I RCCGGY 2 cut(s) 1405, 1473
Bse1I ACTGG 4 cut(s) 317, 371, 812, 1075
Bse8I GATNNNNATC 1 cut(s) 1428
BseAI TCCGGA 2 cut(s) 1031, 1147
BseBI CCWGG 4 cut(s) 33, 186, 783, 910
BseDI CCNNGG 5 cut(s) 31, 32, 454, 553, 860
BseGI GGATG 4 cut(s) 100, 533, 1213, 1429
BseJI GATNNNNATC 1 cut(s) 1428
BseLI CCNNNNNNNGG 5 cut(s) 338, 782, 785, 1073, 1296
BseMII CTCAG 2 cut(s) 789, 1341
BseNI ACTGG 4 cut(s) 317, 371, 812, 1075
BseXI GCAGC 2 cut(s) 139, 256
BsgI GTGCAG 1 cut(s) 146
Bsh1236I CGCG 1 cut(s) 456
BshFI GGCC 7 cut(s) 37, 453, 789, 865, 1377, 1473, 1486
BshNI GGYRCC 1 cut(s) 1170
BshTI ACCGGT 1 cut(s) 1405
BsiHKAI GWGCWC 1 cut(s) 723
BsiHKCI CYCGRG 1 cut(s) 754
BsiSI CCGG 4 cut(s) 1032, 1148, 1406, 1474
BslFI GGGAC 2 cut(s) 159, 648
BslI CCNNNNNNNGG 5 cut(s) 338, 782, 785, 1073, 1296
BsmAI GTCTC 3 cut(s) 357, 700, 816
BsmBI CGTCTC 1 cut(s) 700
BsmFI GGGAC 2 cut(s) 159, 648
BsmI GAATGC 3 cut(s) 257, 588, 1450
BsnI GGCC 7 cut(s) 37, 453, 789, 865, 1377, 1473, 1486
Bso31I GGTCTC 1 cut(s) 816
BsoBI CYCGRG 1 cut(s) 754
Bsp1286I GDGCHC 1 cut(s) 723
Bsp13I TCCGGA 2 cut(s) 1031, 1147
BspACI CCGC 6 cut(s) 454, 456, 726, 838, 855, 1077
BspANI GGCC 7 cut(s) 37, 453, 789, 865, 1377, 1473, 1486
BspCNI CTCAG 2 cut(s) 790, 1342
BspEI TCCGGA 2 cut(s) 1031, 1147
BspFNI CGCG 1 cut(s) 456
BspLI GGNNCC 2 cut(s) 551, 1172
BspMAI CTGCAG 3 cut(s) 274, 340, 1164
BspPI GGATC 4 cut(s) 323, 466, 544, 557
BspQI GCTCTTC 1 cut(s) 1455
BspT107I GGYRCC 1 cut(s) 1170
BspTNI GGTCTC 1 cut(s) 816
BsrFI RCCGGY 2 cut(s) 1405, 1473
BsrI ACTGG 4 cut(s) 317, 371, 812, 1075
BssAI RCCGGY 2 cut(s) 1405, 1473
BssECI CCNNGG 5 cut(s) 31, 32, 454, 553, 860
BssNAI GTATAC 1 cut(s) 1399
BssT1I CCWWGG 2 cut(s) 553, 860
Bst1107I GTATAC 1 cut(s) 1399
Bst2UI CCWGG 4 cut(s) 33, 186, 783, 910
Bst4CI ACNGT 7 cut(s) 481, 639, 749, 1218, 1261, 1276, 1368
Bst6I CTCTTC 1 cut(s) 1455
BstC8I GCNNGC 6 cut(s) 853, 953, 1107, 1231, 1379, 1475
BstDEI CTNAG 2 cut(s) 798, 1350
BstDSI CCRYGG 1 cut(s) 454
BstENI CCTNNNNNAGG 1 cut(s) 336
BstF5I GGATG 4 cut(s) 100, 533, 1213, 1429
BstFNI CGCG 1 cut(s) 456
BstMAI GTCTC 3 cut(s) 357, 700, 816
BstMWI GCNNNNNNNGC 2 cut(s) 1168, 1483
BstNI CCWGG 4 cut(s) 33, 186, 783, 910
BstNSI RCATGY 1 cut(s) 1109
BstPAI GACNNNNGTC 1 cut(s) 1097
BstSCI CCNGG 4 cut(s) 31, 184, 781, 908
BstSFI CTRYAG 4 cut(s) 270, 336, 745, 1160
BstUI CGCG 1 cut(s) 456
BstV1I GCAGC 2 cut(s) 139, 256
BstX2I RGATCY 4 cut(s) 294, 549, 1026, 1346
BstYI RGATCY 4 cut(s) 294, 549, 1026, 1346
BstZ17I GTATAC 1 cut(s) 1399
BsuRI GGCC 7 cut(s) 37, 453, 789, 865, 1377, 1473, 1486
BtgI CCRYGG 1 cut(s) 454
BtsCI GGATG 4 cut(s) 100, 533, 1213, 1429
BtsIMutI CAGTG 6 cut(s) 82, 324, 384, 1068, 1223, 1257
Cac8I GCNNGC 6 cut(s) 853, 953, 1107, 1231, 1379, 1475
Cfr10I RCCGGY 2 cut(s) 1405, 1473
Cfr13I GGNCC 4 cut(s) 35, 773, 788, 857
Cfr42I CCGCGG 1 cut(s) 457
CsiI ACCWGGT 1 cut(s) 781
CspAI ACCGGT 1 cut(s) 1405
CviAII CATG 6 cut(s) 144, 1102, 1106, 1167, 1296, 1416
DdeI CTNAG 2 cut(s) 798, 1350
DraIII CACNNNGTG 1 cut(s) 785
EaeI YGGCCR 1 cut(s) 451
Eam1104I CTCTTC 1 cut(s) 1455
EarI CTCTTC 1 cut(s) 1455
EciI GGCGGA 1 cut(s) 853
Eco130I CCWWGG 2 cut(s) 553, 860
Eco147I AGGCCT 1 cut(s) 865
Eco31I GGTCTC 1 cut(s) 816
Eco47I GGWCC 2 cut(s) 773, 857
Eco57I CTGAAG 1 cut(s) 413
Eco88I CYCGRG 1 cut(s) 754
EcoNI CCTNNNNNAGG 1 cut(s) 336
EcoRI GAATTC 1 cut(s) 312
EcoRII CCWGG 4 cut(s) 31, 184, 781, 908
EcoT14I CCWWGG 2 cut(s) 553, 860
EcoT22I ATGCAT 1 cut(s) 1107
ErhI CCWWGG 2 cut(s) 553, 860
Esp3I CGTCTC 1 cut(s) 700
FaeI CATG 6 cut(s) 147, 1105, 1109, 1170, 1299, 1419
FaqI GGGAC 2 cut(s) 159, 648
FatI CATG 6 cut(s) 143, 1101, 1105, 1166, 1295, 1415
FbaI TGATCA 4 cut(s) 441, 484, 664, 897
FblI GTMKAC 3 cut(s) 304, 804, 1398
Fnu4HI GCNGC 3 cut(s) 128, 270, 454
FokI GGATG 4 cut(s) 107, 520, 1220, 1436
Fsp4HI GCNGC 3 cut(s) 128, 270, 454
FspBI CTAG 2 cut(s) 309, 1362
GluI GCNGC 3 cut(s) 128, 270, 454
GsuI CTGGAG 2 cut(s) 381, 1073
HaeIII GGCC 7 cut(s) 37, 453, 789, 865, 1377, 1473, 1486
HapII CCGG 4 cut(s) 1032, 1148, 1406, 1474
Hin1II CATG 6 cut(s) 147, 1105, 1109, 1170, 1299, 1419
HincII GTYRAC 2 cut(s) 305, 805
HindII GTYRAC 2 cut(s) 305, 805
HinfI GANTC 1 cut(s) 1144
HpaII CCGG 4 cut(s) 1032, 1148, 1406, 1474
HphI GGTGA 3 cut(s) 340, 700, 1395
Hpy166II GTNNAC 5 cut(s) 305, 805, 1214, 1221, 1399
Hpy188I TCNGA 7 cut(s) 225, 393, 509, 1060, 1118, 1351, 1440
Hpy188III TCNNGA 9 cut(s) 292, 547, 620, 756, 815, 1024, 1032, 1090, 1148
Hpy8I GTNNAC 5 cut(s) 305, 805, 1214, 1221, 1399
HpyAV CCTTC 6 cut(s) 129, 258, 388, 436, 578, 991
HpyCH4III ACNGT 7 cut(s) 481, 639, 749, 1218, 1261, 1276, 1368
HpyF10VI GCNNNNNNNGC 2 cut(s) 1168, 1483
HpyF3I CTNAG 2 cut(s) 798, 1350
Hsp92II CATG 6 cut(s) 147, 1105, 1109, 1170, 1299, 1419
Kpn2I TCCGGA 2 cut(s) 1031, 1147
KroI GCCGGC 1 cut(s) 1473
KroNI GCCGGC 1 cut(s) 1475
Ksp22I TGATCA 4 cut(s) 441, 484, 664, 897
KspI CCGCGG 1 cut(s) 457
LguI GCTCTTC 1 cut(s) 1455
LmnI GCTCC 1 cut(s) 940
Lsp1109I GCAGC 2 cut(s) 139, 256
LweI GCATC 3 cut(s) 555, 1062, 1475
MabI ACCWGGT 1 cut(s) 781
MaeI CTAG 2 cut(s) 309, 1362
MaeIII GTNAC 6 cut(s) 52, 233, 557, 639, 655, 749
MboII GAAGA 6 cut(s) 200, 413, 731, 1382, 1442, 1453
MflI RGATCY 4 cut(s) 294, 549, 1026, 1346
MhlI GDGCHC 1 cut(s) 723
MmeI TCCRAC 3 cut(s) 502, 1012, 1027
Mph1103I ATGCAT 1 cut(s) 1107
MroI TCCGGA 2 cut(s) 1031, 1147
MroNI GCCGGC 1 cut(s) 1473
MroXI GAANNNNTTC 2 cut(s) 102, 588
MseI TTAA 1 cut(s) 1238
MslI CAYNNNNRTG 1 cut(s) 1176
MspA1I CMGCKG 2 cut(s) 456, 662
MspI CCGG 4 cut(s) 1032, 1148, 1406, 1474
MspR9I CCNGG 4 cut(s) 33, 186, 783, 910
Mva1269I GAATGC 3 cut(s) 257, 588, 1450
MvaI CCWGG 4 cut(s) 33, 186, 783, 910
MvnI CGCG 1 cut(s) 456
MwoI GCNNNNNNNGC 2 cut(s) 1168, 1483
NaeI GCCGGC 1 cut(s) 1475
NgoMIV GCCGGC 1 cut(s) 1473
NlaIII CATG 6 cut(s) 147, 1105, 1109, 1170, 1299, 1419
NlaIV GGNNCC 2 cut(s) 551, 1172
NmeAIII GCCGAG 1 cut(s) 1462
NmuCI GTSAC 3 cut(s) 52, 557, 639
NsiI ATGCAT 1 cut(s) 1107
NspI RCATGY 1 cut(s) 1109
PaeI GCATGC 1 cut(s) 1109
PasI CCCWGGG 1 cut(s) 32
PceI AGGCCT 1 cut(s) 865
PciSI GCTCTTC 1 cut(s) 1455
PctI GAATGC 3 cut(s) 257, 588, 1450
PdiI GCCGGC 1 cut(s) 1475
PdmI GAANNNNTTC 2 cut(s) 102, 588
PfeI GAWTC 1 cut(s) 1144
PflMI CCANNNNNTGG 2 cut(s) 785, 1296
PinAI ACCGGT 1 cut(s) 1405
PkrI GCNGC 3 cut(s) 129, 271, 455
PshAI GACNNNNGTC 1 cut(s) 1097
Psp6I CCWGG 4 cut(s) 31, 184, 781, 908
PspGI CCWGG 4 cut(s) 31, 184, 781, 908
PspN4I GGNNCC 2 cut(s) 551, 1172
PspPI GGNCC 4 cut(s) 35, 773, 788, 857
PstI CTGCAG 3 cut(s) 274, 340, 1164
PsuI RGATCY 4 cut(s) 294, 549, 1026, 1346
PvuII CAGCTG 1 cut(s) 662
RseI CAYNNNNRTG 1 cut(s) 1176
SacII CCGCGG 1 cut(s) 457
SalI GTCGAC 2 cut(s) 303, 803
SapI GCTCTTC 1 cut(s) 1455
SaqAI TTAA 1 cut(s) 1238
SatI GCNGC 3 cut(s) 128, 270, 454
Sau96I GGNCC 4 cut(s) 35, 773, 788, 857
SbfI CCTGCAGG 1 cut(s) 340
ScrFI CCNGG 4 cut(s) 33, 186, 783, 910
SdaI CCTGCAGG 1 cut(s) 340
SduI GDGCHC 1 cut(s) 723
SexAI ACCWGGT 1 cut(s) 781
SfaNI GCATC 3 cut(s) 555, 1062, 1475
SfcI CTRYAG 4 cut(s) 270, 336, 745, 1160
Sfr303I CCGCGG 1 cut(s) 457
SgrBI CCGCGG 1 cut(s) 457
SinI GGWCC 2 cut(s) 773, 857
SmiMI CAYNNNNRTG 1 cut(s) 1176
SmlI CTYRAG 2 cut(s) 298, 873
SmoI CTYRAG 2 cut(s) 298, 873
SphI GCATGC 1 cut(s) 1109
Sse8387I CCTGCAGG 1 cut(s) 340
SseBI AGGCCT 1 cut(s) 865
SsiI CCGC 6 cut(s) 454, 456, 726, 838, 855, 1077
SspI AATATT 1 cut(s) 12
SspMI CTAG 2 cut(s) 309, 1362
StuI AGGCCT 1 cut(s) 865
StyD4I CCNGG 4 cut(s) 31, 184, 781, 908
StyI CCWWGG 2 cut(s) 553, 860
TaaI ACNGT 7 cut(s) 481, 639, 749, 1218, 1261, 1276, 1368
TaqI TCGA 4 cut(s) 304, 804, 826, 1462
TauI GCSGC 1 cut(s) 456
TfiI GAWTC 1 cut(s) 1144
Tru1I TTAA 1 cut(s) 1238
Tru9I TTAA 1 cut(s) 1238
TscAI CASTG 6 cut(s) 82, 324, 391, 1075, 1223, 1264
TseFI GTSAC 3 cut(s) 52, 557, 639
TseI GCWGC 2 cut(s) 127, 269
Tsp45I GTSAC 3 cut(s) 52, 557, 639
TspDTI ATGAA 4 cut(s) 111, 617, 1404, 1440
TspGWI ACGGA 2 cut(s) 450, 1033
TspRI CASTG 6 cut(s) 82, 324, 391, 1075, 1223, 1264
Van91I CCANNNNNTGG 2 cut(s) 785, 1296
VpaK11BI GGWCC 2 cut(s) 773, 857
XagI CCTNNNNNAGG 1 cut(s) 336
XapI RAATTY 5 cut(s) 312, 841, 928, 1130, 1411
XceI RCATGY 1 cut(s) 1109
XmiI GTMKAC 3 cut(s) 304, 804, 1398
XmnI GAANNNNTTC 2 cut(s) 102, 588
XspI CTAG 2 cut(s) 309, 1362
Zsp2I ATGCAT 1 cut(s) 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.