Rh2BG552700

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0013817
Physical Location & Seq
Reverse (-)
76104428 .. 76108993
4566 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG552700.1

Sequence Viewer

Length: 1140 bp
ATGTTAGTTGATGGGCTTGTCTGTATGTTACAGGATTTGAAGGCAATTTTAGATGGTGGGAATAATCTTCCCTTCCCCGACGGACTTCTCATCAATGGCCGAGGATCAAATGGGAATACATATACAGTTGATCAAGGCAACACTTACAGGTTCAGAATATCAAATGTTGGAATTGCAACATCCATCAATTTCAGAATCCAAGGTCACAAGATGCTGCTGGTTGAGGTGGAAGGCATTCACACCTTGCAAAACACGTACAGCTCTCTTGACATTCATTTGGGGCAGTCCTACTCTGTTCTGGTCACAGCTGATCAACCACCACAAGATTACTACATTGTTGTCTCTACGCGTTTCACTTCACAAGTGCTCTCTGCAACCTCCATTCTTCACTACAGTAACTCAGCGGGAAGTGTTTCTGGTCCTCCACCTGGTGGCCCAACTACTCAGGTTGACTGGTCTCTTGAACAGGCTCGGTCTTTCAGGCGGAATTTGACAGCAAGCGGACCGAGGCCTAACCCTCAAGGCTCATATCATTATGGATTGATCAACACCACCCGTACAATCAGGCTTACAAACTCTGCTCCGTTAATCAATGGCAAGCAAAGATATGCTGTAAATAGTGTCTCCTTCCTCCAACCTGACACACCACTTAAAGTTGCTGACTACTTCAAGATCTCCGGAGTATTCTCCATTGGAAGCATCACAGATAGCCCCACTGGTGGTGGTGCTTATCTCCAGACTTCTGTCATGCATGCTGATTTCCGAGCCTTTGTTGAACTCGTGTTTGAGAATCCGGAGGAGACTTTGCAGTCATGGCACATTGATGGTTATAGTTTCTTTGTTGTTGGTATGGATGGTGGAGAGTGGACACCAGCAAGCAGATTAAGATACAATCTGCAGGACACTGTTTCTCGTTGCACTGTTCAGGTGTATCCCAACTCATGGACTGCAATTTACGTGCCTTTGGATAATGTGGGAATGTGGAATGTGAGATCTGAGAATTGGGCAAGACAGTACTTAGGCCAGCAGTTCTATCTTCGTGTATACTCACCAGCGAATTCATGGAGAGATGAATATCCAATTCCAAAGAATGCACTTCTGTGTGGTCGGGCATCAGGCCGGCGAACTCGGCCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

379

Amino Acids

42.09

Weight (kDa)

8.33

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase PF00394 19 - 134 2.9e-36 Multicopper oxidase
Cu-oxidase_2 PF07731 214 - 351 7.2e-32 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013817)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g42990 FvH4_6g42990
malus_domestica MD09G1110500.v1.1 MD17G1098300.v1.1
prunus_persica Prupe.3G216000_v2.0.a1
pyrus_communis pycom09g03290 pycom17g09370
rosa_chinensis RchiOBHm_Chr2g0158971
rosa_laevigata RLG00000021129
rosa_multiflora Rmu_sc0007907.1_g000005
rosa_roxburghii Rroxscaffold_2G00090830
rosa_rugosa Rorug02G0473300
rosa_samantha Rh2AG539900 Rh2BG552700 Rh2CG523200 Rh2DG562500
rosa_wichuraiana Rw2G044690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 808
AccB7I CCANNNNNTGG 2 cut(s) 431, 942
AccI GTMKAC 1 cut(s) 1044
AccII CGCG 1 cut(s) 349
AccIII TCCGGA 2 cut(s) 677, 793
AciI CCGC 3 cut(s) 404, 484, 501
AclWI GGATC 1 cut(s) 112
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 2 cut(s) 487, 1057
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 3 cut(s) 257, 559, 1016
AfiI CCNNNNNNNGG 4 cut(s) 428, 431, 719, 942
AflIII ACRYGT 2 cut(s) 252, 347
AgsI TTSAA 4 cut(s) 40, 464, 670, 776
AjnI CCWGG 1 cut(s) 427
AleI CACNNNNGTG 1 cut(s) 1099
AluBI AGCT 2 cut(s) 261, 308
AluI AGCT 2 cut(s) 261, 308
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 4 cut(s) 346, 462, 628, 794
AlwI GGATC 1 cut(s) 112
Aor13HI TCCGGA 2 cut(s) 677, 793
AoxI GGCC 6 cut(s) 97, 433, 509, 1021, 1117, 1130
ApeKI GCWGC 1 cut(s) 214
ApoI RAATTY 2 cut(s) 487, 1057
Asp700I GAANNNNTTC 2 cut(s) 234, 412
AspS9I GGNCC 3 cut(s) 419, 434, 503
AsuHPI GGTGA 1 cut(s) 1041
AvaII GGWCC 2 cut(s) 419, 503
BauI CACGAG 1 cut(s) 779
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 201
BccI CCATC 5 cut(s) 5, 47, 191, 818, 848
BciT130I CCWGG 1 cut(s) 429
BciVI GTATCC 1 cut(s) 942
BclI TGATCA 3 cut(s) 130, 310, 543
BcoDI GTCTC 4 cut(s) 346, 462, 628, 794
BfmI CTRYAG 2 cut(s) 391, 896
BfuI GTATCC 1 cut(s) 942
BglII AGATCT 2 cut(s) 672, 992
BisI GCNGC 1 cut(s) 215
BlsI GCNGC 1 cut(s) 216
BmcAI AGTACT 1 cut(s) 1016
Bme1390I CCNGG 1 cut(s) 429
Bme18I GGWCC 2 cut(s) 419, 503
BmgT120I GGNCC 3 cut(s) 419, 434, 503
BmrFI CCNGG 1 cut(s) 429
BmsI GCATC 3 cut(s) 201, 708, 1121
BoxI GACNNNNGTC 1 cut(s) 743
BpmI CTGGAG 1 cut(s) 719
BpuEI CTTGAG 1 cut(s) 504
BsaAI YACGTR 2 cut(s) 255, 958
BsaBI GATNNNNATC 1 cut(s) 1074
BsaI GGTCTC 1 cut(s) 462
BsaJI CCNNGG 3 cut(s) 100, 199, 506
BsaWI WCCGGW 2 cut(s) 677, 793
Bsc4I CCNNNNNNNGG 4 cut(s) 428, 431, 719, 942
Bse118I RCCGGY 1 cut(s) 1119
Bse1I ACTGG 2 cut(s) 458, 721
Bse8I GATNNNNATC 1 cut(s) 1074
BseAI TCCGGA 2 cut(s) 677, 793
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 3 cut(s) 100, 199, 506
BseGI GGATG 2 cut(s) 179, 859
BseJI GATNNNNATC 1 cut(s) 1074
BseLI CCNNNNNNNGG 4 cut(s) 428, 431, 719, 942
BseMII CTCAG 3 cut(s) 414, 458, 987
BseNI ACTGG 2 cut(s) 458, 721
BseRI GAGGAG 1 cut(s) 812
BseXI GCAGC 1 cut(s) 201
Bsh1236I CGCG 1 cut(s) 349
BshFI GGCC 6 cut(s) 99, 435, 511, 1023, 1119, 1132
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 3 cut(s) 678, 794, 1120
BslI CCNNNNNNNGG 4 cut(s) 428, 431, 719, 942
BsmAI GTCTC 4 cut(s) 346, 462, 628, 794
BsmI GAATGC 2 cut(s) 234, 1096
BsnI GGCC 6 cut(s) 99, 435, 511, 1023, 1119, 1132
Bso31I GGTCTC 1 cut(s) 462
Bsp1286I GDGCHC 1 cut(s) 369
Bsp13I TCCGGA 2 cut(s) 677, 793
Bsp143I GATC 6 cut(s) 104, 130, 310, 543, 672, 992
BspACI CCGC 3 cut(s) 404, 484, 501
BspANI GGCC 6 cut(s) 99, 435, 511, 1023, 1119, 1132
BspCNI CTCAG 3 cut(s) 413, 457, 988
BspEI TCCGGA 2 cut(s) 677, 793
BspFNI CGCG 1 cut(s) 349
BspMAI CTGCAG 1 cut(s) 900
BspPI GGATC 1 cut(s) 112
BspTNI GGTCTC 1 cut(s) 462
BsrFI RCCGGY 1 cut(s) 1119
BsrI ACTGG 2 cut(s) 458, 721
BssAI RCCGGY 1 cut(s) 1119
BssECI CCNNGG 3 cut(s) 100, 199, 506
BssMI GATC 6 cut(s) 104, 130, 310, 543, 672, 992
BssNAI GTATAC 1 cut(s) 1045
BssSI CACGAG 1 cut(s) 779
BssT1I CCWWGG 1 cut(s) 199
Bst1107I GTATAC 1 cut(s) 1045
Bst2BI CACGAG 1 cut(s) 779
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 5 cut(s) 127, 395, 907, 922, 1014
BstBAI YACGTR 2 cut(s) 255, 958
BstC8I GCNNGC 6 cut(s) 499, 599, 753, 877, 1025, 1121
BstDEI CTNAG 4 cut(s) 400, 444, 996, 1018
BstF5I GGATG 2 cut(s) 179, 859
BstFNI CGCG 1 cut(s) 349
BstKTI GATC 6 cut(s) 107, 133, 313, 546, 675, 995
BstMAI GTCTC 4 cut(s) 346, 462, 628, 794
BstMBI GATC 6 cut(s) 104, 130, 310, 543, 672, 992
BstMWI GCNNNNNNNGC 2 cut(s) 814, 1129
BstNI CCWGG 1 cut(s) 429
BstNSI RCATGY 1 cut(s) 755
BstPAI GACNNNNGTC 1 cut(s) 743
BstSCI CCNGG 1 cut(s) 427
BstSFI CTRYAG 2 cut(s) 391, 896
BstUI CGCG 1 cut(s) 349
BstV1I GCAGC 1 cut(s) 201
BstX2I RGATCY 2 cut(s) 672, 992
BstYI RGATCY 2 cut(s) 672, 992
BstZ17I GTATAC 1 cut(s) 1045
BsuI GTATCC 1 cut(s) 942
BsuRI GGCC 6 cut(s) 99, 435, 511, 1023, 1119, 1132
BtsCI GGATG 2 cut(s) 179, 859
BtsIMutI CAGTG 3 cut(s) 714, 903, 918
Cac8I GCNNGC 6 cut(s) 499, 599, 753, 877, 1025, 1121
Cfr10I RCCGGY 1 cut(s) 1119
Cfr13I GGNCC 3 cut(s) 419, 434, 503
CpoI CGGWCCG 1 cut(s) 503
CsiI ACCWGGT 1 cut(s) 427
Csp6I GTAC 3 cut(s) 256, 558, 1015
CspI CGGWCCG 1 cut(s) 503
CviAII CATG 5 cut(s) 748, 752, 813, 942, 1062
CviQI GTAC 3 cut(s) 256, 558, 1015
DdeI CTNAG 4 cut(s) 400, 444, 996, 1018
DpnI GATC 6 cut(s) 106, 132, 312, 545, 674, 994
DpnII GATC 6 cut(s) 104, 130, 310, 543, 672, 992
DraIII CACNNNGTG 1 cut(s) 431
DrdI GACNNNNNNGTC 1 cut(s) 808
DseDI GACNNNNNNGTC 1 cut(s) 808
EaeI YGGCCR 1 cut(s) 97
EciI GGCGGA 1 cut(s) 499
Eco130I CCWWGG 1 cut(s) 199
Eco147I AGGCCT 1 cut(s) 511
Eco31I GGTCTC 1 cut(s) 462
Eco47I GGWCC 2 cut(s) 419, 503
EcoRI GAATTC 1 cut(s) 1057
EcoRII CCWGG 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 199
EcoT22I ATGCAT 1 cut(s) 753
ErhI CCWWGG 1 cut(s) 199
FaeI CATG 5 cut(s) 751, 755, 816, 945, 1065
FatI CATG 5 cut(s) 747, 751, 812, 941, 1061
FauI CCCGC 1 cut(s) 397
FbaI TGATCA 3 cut(s) 130, 310, 543
FblI GTMKAC 1 cut(s) 1044
Fnu4HI GCNGC 1 cut(s) 215
FokI GGATG 2 cut(s) 166, 866
Fsp4HI GCNGC 1 cut(s) 215
GluI GCNGC 1 cut(s) 215
GsuI CTGGAG 1 cut(s) 719
HaeIII GGCC 6 cut(s) 99, 435, 511, 1023, 1119, 1132
HapII CCGG 3 cut(s) 678, 794, 1120
Hin1II CATG 5 cut(s) 751, 755, 816, 945, 1065
HincII GTYRAC 1 cut(s) 451
HindII GTYRAC 1 cut(s) 451
HinfI GANTC 2 cut(s) 195, 790
HpaII CCGG 3 cut(s) 678, 794, 1120
HphI GGTGA 1 cut(s) 1041
Hpy166II GTNNAC 3 cut(s) 451, 867, 1045
Hpy188I TCNGA 4 cut(s) 155, 194, 764, 997
Hpy188III TCNNGA 6 cut(s) 266, 461, 670, 678, 736, 794
Hpy8I GTNNAC 3 cut(s) 451, 867, 1045
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 4 cut(s) 34, 82, 224, 637
HpyCH4III ACNGT 5 cut(s) 127, 395, 907, 922, 1014
HpyCH4IV ACGT 2 cut(s) 254, 957
HpyCH4V TGCA 9 cut(s) 176, 247, 374, 751, 808, 898, 918, 950, 1094
HpyF10VI GCNNNNNNNGC 2 cut(s) 814, 1129
HpyF3I CTNAG 4 cut(s) 400, 444, 996, 1018
HpySE526I ACGT 2 cut(s) 254, 957
Hsp92II CATG 5 cut(s) 751, 755, 816, 945, 1065
Kpn2I TCCGGA 2 cut(s) 677, 793
KroI GCCGGC 1 cut(s) 1119
KroNI GCCGGC 1 cut(s) 1121
Ksp22I TGATCA 3 cut(s) 130, 310, 543
Kzo9I GATC 6 cut(s) 104, 130, 310, 543, 672, 992
LmnI GCTCC 1 cut(s) 586
Lsp1109I GCAGC 1 cut(s) 201
LweI GCATC 3 cut(s) 201, 708, 1121
MabI ACCWGGT 1 cut(s) 427
MaeII ACGT 2 cut(s) 254, 957
MaeIII GTNAC 4 cut(s) 27, 203, 301, 395
MalI GATC 6 cut(s) 106, 132, 312, 545, 674, 994
MboI GATC 6 cut(s) 104, 130, 310, 543, 672, 992
MboII GAAGA 3 cut(s) 59, 377, 1028
MflI RGATCY 2 cut(s) 672, 992
MhlI GDGCHC 1 cut(s) 369
MluCI AATT 8 cut(s) 45, 171, 187, 487, 951, 1000, 1057, 1080
MluI ACGCGT 1 cut(s) 347
MmeI TCCRAC 2 cut(s) 148, 658
MnlI CCTC 8 cut(s) 95, 217, 388, 432, 501, 528, 641, 790
Mph1103I ATGCAT 1 cut(s) 753
MroI TCCGGA 2 cut(s) 677, 793
MroNI GCCGGC 1 cut(s) 1119
MroXI GAANNNNTTC 2 cut(s) 234, 412
MseI TTAA 3 cut(s) 587, 651, 884
MslI CAYNNNNRTG 2 cut(s) 822, 1099
MspA1I CMGCKG 2 cut(s) 308, 404
MspI CCGG 3 cut(s) 678, 794, 1120
MspR9I CCNGG 1 cut(s) 429
Mva1269I GAATGC 2 cut(s) 234, 1096
MvaI CCWGG 1 cut(s) 429
MvnI CGCG 1 cut(s) 349
MwoI GCNNNNNNNGC 2 cut(s) 814, 1129
NaeI GCCGGC 1 cut(s) 1121
NdeII GATC 6 cut(s) 104, 130, 310, 543, 672, 992
NgoMIV GCCGGC 1 cut(s) 1119
NlaIII CATG 5 cut(s) 751, 755, 816, 945, 1065
NmeAIII GCCGAG 2 cut(s) 125, 1108
NmuCI GTSAC 2 cut(s) 203, 301
NsiI ATGCAT 1 cut(s) 753
NspI RCATGY 1 cut(s) 755
OliI CACNNNNGTG 1 cut(s) 1099
PaeI GCATGC 1 cut(s) 755
PceI AGGCCT 1 cut(s) 511
PctI GAATGC 2 cut(s) 234, 1096
PdiI GCCGGC 1 cut(s) 1121
PdmI GAANNNNTTC 2 cut(s) 234, 412
PfeI GAWTC 2 cut(s) 195, 790
PflMI CCANNNNNTGG 2 cut(s) 431, 942
PkrI GCNGC 1 cut(s) 216
Ppu21I YACGTR 2 cut(s) 255, 958
PshAI GACNNNNGTC 1 cut(s) 743
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PspPI GGNCC 3 cut(s) 419, 434, 503
PstI CTGCAG 1 cut(s) 900
PsuI RGATCY 2 cut(s) 672, 992
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 3 cut(s) 257, 559, 1016
RsaNI GTAC 3 cut(s) 256, 558, 1015
RseI CAYNNNNRTG 2 cut(s) 822, 1099
Rsr2I CGGWCCG 1 cut(s) 503
RsrII CGGWCCG 1 cut(s) 503
SaqAI TTAA 3 cut(s) 587, 651, 884
SatI GCNGC 1 cut(s) 215
Sau3AI GATC 6 cut(s) 104, 130, 310, 543, 672, 992
Sau96I GGNCC 3 cut(s) 419, 434, 503
ScaI AGTACT 1 cut(s) 1016
ScrFI CCNGG 1 cut(s) 429
SduI GDGCHC 1 cut(s) 369
SexAI ACCWGGT 1 cut(s) 427
SfaNI GCATC 3 cut(s) 201, 708, 1121
SfcI CTRYAG 2 cut(s) 391, 896
SinI GGWCC 2 cut(s) 419, 503
SmiMI CAYNNNNRTG 2 cut(s) 822, 1099
SmlI CTYRAG 1 cut(s) 519
SmoI CTYRAG 1 cut(s) 519
SphI GCATGC 1 cut(s) 755
Sse9I AATT 8 cut(s) 45, 171, 187, 487, 951, 1000, 1057, 1080
SseBI AGGCCT 1 cut(s) 511
SsiI CCGC 3 cut(s) 404, 484, 501
StuI AGGCCT 1 cut(s) 511
StyD4I CCNGG 1 cut(s) 427
StyI CCWWGG 1 cut(s) 199
TaaI ACNGT 5 cut(s) 127, 395, 907, 922, 1014
TaiI ACGT 2 cut(s) 257, 960
TaqII GACCGA 2 cut(s) 462, 520
TasI AATT 8 cut(s) 45, 171, 187, 487, 951, 1000, 1057, 1080
TatI WGTACW 1 cut(s) 1014
TfiI GAWTC 2 cut(s) 195, 790
Tru1I TTAA 3 cut(s) 587, 651, 884
Tru9I TTAA 3 cut(s) 587, 651, 884
TscAI CASTG 3 cut(s) 721, 910, 925
TseFI GTSAC 2 cut(s) 203, 301
TseI GCWGC 1 cut(s) 214
Tsp45I GTSAC 2 cut(s) 203, 301
TspDTI ATGAA 3 cut(s) 263, 1050, 1086
TspGWI ACGGA 2 cut(s) 96, 573
TspRI CASTG 3 cut(s) 721, 910, 925
Van91I CCANNNNNTGG 2 cut(s) 431, 942
VpaK11BI GGWCC 2 cut(s) 419, 503
XapI RAATTY 2 cut(s) 487, 1057
XceI RCATGY 1 cut(s) 755
XcmI CCANNNNNNNNNTGG 1 cut(s) 1059
XmiI GTMKAC 1 cut(s) 1044
XmnI GAANNNNTTC 2 cut(s) 234, 412
ZrmI AGTACT 1 cut(s) 1016
Zsp2I ATGCAT 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.