Rh2DG562500

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0013817
Physical Location & Seq
Reverse (-)
79133209 .. 79137764
4556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG562500.1

Sequence Viewer

Length: 1506 bp
ATGGGACTCTACACAAGTGTCTGTTGTGTCTTATTGGTTGCTTTGCTTACTGTGAGCCTCACTAATGGGGAAGATCCATATAGGTTCTACAACTGGAATGTCACCTATGGTGATATCTATCCCCTTGGTGTAAAACAGCAGGGGATATTGATAAATGGGCAATTCCCGGGGCCACAGATTGATTCTGTCACCAATGATAACCTTATTATCAGTGTTTTCAACAGCTTGGATGAACCTTTTCTCCTTTCTTGGAATGGTGTGCAGCAGAGAAGGAACTCATGGCAAGATGGGGTCTTTGGCACAAATTGTCCCATCCCACCAGGGCAGAACTTCACCTATGTTCTCCAGGTCAAGGATCAAATTGGAAGTTACTTTTACTTTCCTTCCCTTGCCTTCCACAAAGCTGCAGGAGGATTTGGTGGCATCAAAATAGCAAGCCGACCTCGTATTCCGGTGCCTTTTCCTCCTCCTGCTGGAGATTTCACCATTCTGGCTGGAGACTGGTTCAAGCAAAATCACACTGATTTGAAGGCAATTTTAGATGGTGGGAATAATCTTCCCTTCCCCGACGGACTTCTCATCAATGGCCGAGGATCAAATGGGAATACATATACAGTTGATCAAGGCAACACTTACAGGTTCAGAATATCAAATGTTGGAATTGCAACATCCATCAATTTCAGAATCCAAGGTCACAAGATGCTGCTGGTTGAGGTGGAAGGCATTCACACCTTGCAAAACACGTACAGCTCTCTTGACATTCATTTGGGGCAGTCCTACTCTGTTCTGGTCACAGCTGATCAACCACCGCAAGATTACTACATTGTTGTCTCTACGCGTTTCACTTCACAAGTGCTCTCTGCAACCTCCATTCTTCACTACAGTAACTCAGCGGGAAGTGTTTCTGGTCCTCCACCTGGTGGCCCAACTACTCAGGTTGACTGGTCTCTTGAACAGGCTCGGTCTTTCAGGCGGAATTTGACAGCAAGCGGACCGAGGCCTAACCCTCAAGGCTCATATCATTATGGATTGATCAACACCACCCGTACAATCAGGCTTACAAACTCTGCTCCGTTAATCAATGGCAAGCAAAGATATGCTGTAAATAGTGTCTCCTTCCTCCAACCTGACACACCACTTAAAGTTGCTGACTACTTCAAGATCTCCGGAGTATTCTCCATTGGAAGCATCACAGATAGCCCCACTGGTGGTGGTGCTTATCTCCAGACTTCTGTCATGCATGCTGATTTCCGAGCCTTTGTTGAACTCGTGTTTGAGAATCCGGAGGAGACTTTGCAGTCATGGCACATTGATGGTTATAGTTTCTTTGTTGTTGGTATGGATGGTGGAGAGTGGACACCAGCAAGCAGATTAAGATACAATCTGCAGGACACTGTTTCTCGTTGCACTGTTCAGGTAAACTCAACAAATATTTCCCAAATATTCTTACACATGACAAAAAAAATTTGGCTAAGTTTAGCAGTAGTTGTCCTAATAACTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

501

Amino Acids

55.2

Weight (kDa)

6.79

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 147 6.2e-39 Multicopper oxidase
Cu-oxidase PF00394 160 - 297 3.6e-42 Multicopper oxidase
Cu-oxidase_2 PF07731 377 - 475 6.3e-19 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013817)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g42990 FvH4_6g42990
malus_domestica MD09G1110500.v1.1 MD17G1098300.v1.1
prunus_persica Prupe.3G216000_v2.0.a1
pyrus_communis pycom09g03290 pycom17g09370
rosa_chinensis RchiOBHm_Chr2g0158971
rosa_laevigata RLG00000021129
rosa_multiflora Rmu_sc0007907.1_g000005
rosa_roxburghii Rroxscaffold_2G00090830
rosa_rugosa Rorug02G0473300
rosa_samantha Rh2AG539900 Rh2BG552700 Rh2CG523200 Rh2DG562500
rosa_wichuraiana Rw2G044690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1297
AccB1I GGYRCC 1 cut(s) 454
AccB7I CCANNNNNTGG 1 cut(s) 920
AccII CGCG 1 cut(s) 838
AccIII TCCGGA 2 cut(s) 1166, 1282
AciI CCGC 4 cut(s) 809, 893, 973, 990
AclWI GGATC 3 cut(s) 68, 363, 601
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 2 cut(s) 976, 1464
AdeI CACNNNGTG 1 cut(s) 920
AfaI GTAC 2 cut(s) 746, 1048
AfiI CCNNNNNNNGG 5 cut(s) 352, 473, 917, 920, 1208
AflIII ACRYGT 2 cut(s) 741, 836
AgsI TTSAA 6 cut(s) 220, 508, 529, 953, 1159, 1265
AjnI CCWGG 3 cut(s) 319, 345, 916
AloI GAACNNNNNNTCC 2 cut(s) 225, 257
AluBI AGCT 4 cut(s) 225, 404, 750, 797
AluI AGCT 4 cut(s) 225, 404, 750, 797
Alw21I GWGCWC 1 cut(s) 858
Alw26I GTCTC 5 cut(s) 492, 835, 951, 1117, 1283
AlwI GGATC 3 cut(s) 68, 363, 601
Ama87I CYCGRG 1 cut(s) 166
Aor13HI TCCGGA 2 cut(s) 1166, 1282
AoxI GGCC 4 cut(s) 170, 586, 922, 998
ApeKI GCWGC 3 cut(s) 262, 404, 703
ApoI RAATTY 2 cut(s) 976, 1464
Asp700I GAANNNNTTC 3 cut(s) 237, 723, 901
AspS9I GGNCC 4 cut(s) 170, 908, 923, 992
AsuC2I CCSGG 2 cut(s) 167, 168
AsuHPI GGTGA 5 cut(s) 94, 122, 181, 325, 475
AvaI CYCGRG 1 cut(s) 166
AvaII GGWCC 2 cut(s) 908, 992
BanI GGYRCC 1 cut(s) 454
BauI CACGAG 1 cut(s) 1268
Bbv12I GWGCWC 1 cut(s) 858
BbvI GCAGC 3 cut(s) 274, 391, 690
BccI CCATC 6 cut(s) 281, 320, 536, 680, 1307, 1337
BciT130I CCWGG 3 cut(s) 321, 347, 918
BclI TGATCA 3 cut(s) 619, 799, 1032
BcnI CCSGG 2 cut(s) 167, 168
BcoDI GTCTC 5 cut(s) 492, 835, 951, 1117, 1283
BfmI CTRYAG 3 cut(s) 405, 880, 1385
BglII AGATCT 1 cut(s) 1161
BisI GCNGC 3 cut(s) 263, 405, 704
BlsI GCNGC 3 cut(s) 264, 406, 705
Bme1390I CCNGG 5 cut(s) 167, 168, 321, 347, 918
Bme18I GGWCC 2 cut(s) 908, 992
BmeT110I CYCGRG 1 cut(s) 166
BmgT120I GGNCC 4 cut(s) 170, 908, 923, 992
BmiI GGNNCC 2 cut(s) 171, 456
BmrFI CCNGG 5 cut(s) 167, 168, 321, 347, 918
BmsI GCATC 3 cut(s) 432, 690, 1197
BoxI GACNNNNGTC 1 cut(s) 1232
BpmI CTGGAG 4 cut(s) 329, 495, 516, 1208
BpuEI CTTGAG 1 cut(s) 993
BpuMI CCSGG 2 cut(s) 167, 168
BsaAI YACGTR 1 cut(s) 744
BsaBI GATNNNNATC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 951
BsaJI CCNNGG 7 cut(s) 124, 166, 167, 320, 589, 688, 995
BsaWI WCCGGW 3 cut(s) 451, 1166, 1282
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bsc4I CCNNNNNNNGG 5 cut(s) 352, 473, 917, 920, 1208
Bse1I ACTGG 4 cut(s) 98, 506, 947, 1210
Bse8I GATNNNNATC 1 cut(s) 117
BseAI TCCGGA 2 cut(s) 1166, 1282
BseBI CCWGG 3 cut(s) 321, 347, 918
BseDI CCNNGG 7 cut(s) 124, 166, 167, 320, 589, 688, 995
BseGI GGATG 4 cut(s) 235, 312, 668, 1348
BseJI GATNNNNATC 1 cut(s) 117
BseLI CCNNNNNNNGG 5 cut(s) 352, 473, 917, 920, 1208
BseMII CTCAG 2 cut(s) 903, 947
BseNI ACTGG 4 cut(s) 98, 506, 947, 1210
BseRI GAGGAG 2 cut(s) 456, 1301
BseXI GCAGC 3 cut(s) 274, 391, 690
BsgI GTGCAG 1 cut(s) 281
Bsh1236I CGCG 1 cut(s) 838
BshFI GGCC 4 cut(s) 172, 588, 924, 1000
BshNI GGYRCC 1 cut(s) 454
BsiHKAI GWGCWC 1 cut(s) 858
BsiHKCI CYCGRG 1 cut(s) 166
BsiSI CCGG 4 cut(s) 167, 452, 1167, 1283
BslFI GGGAC 2 cut(s) 18, 294
BslI CCNNNNNNNGG 5 cut(s) 352, 473, 917, 920, 1208
BsmAI GTCTC 5 cut(s) 492, 835, 951, 1117, 1283
BsmFI GGGAC 2 cut(s) 18, 294
BsmI GAATGC 1 cut(s) 723
BsnI GGCC 4 cut(s) 172, 588, 924, 1000
Bso31I GGTCTC 1 cut(s) 951
BsoBI CYCGRG 1 cut(s) 166
Bsp1286I GDGCHC 1 cut(s) 858
Bsp13I TCCGGA 2 cut(s) 1166, 1282
Bsp143I GATC 7 cut(s) 73, 355, 593, 619, 799, 1032, 1161
BspACI CCGC 4 cut(s) 809, 893, 973, 990
BspANI GGCC 4 cut(s) 172, 588, 924, 1000
BspCNI CTCAG 2 cut(s) 902, 946
BspEI TCCGGA 2 cut(s) 1166, 1282
BspFNI CGCG 1 cut(s) 838
BspLI GGNNCC 2 cut(s) 171, 456
BspMAI CTGCAG 2 cut(s) 409, 1389
BspPI GGATC 3 cut(s) 68, 363, 601
BspT107I GGYRCC 1 cut(s) 454
BspTNI GGTCTC 1 cut(s) 951
BsrI ACTGG 4 cut(s) 98, 506, 947, 1210
BssECI CCNNGG 7 cut(s) 124, 166, 167, 320, 589, 688, 995
BssMI GATC 7 cut(s) 73, 355, 593, 619, 799, 1032, 1161
BssSI CACGAG 1 cut(s) 1268
BssT1I CCWWGG 2 cut(s) 124, 688
Bst2BI CACGAG 1 cut(s) 1268
Bst2UI CCWGG 3 cut(s) 321, 347, 918
Bst4CI ACNGT 5 cut(s) 52, 616, 884, 1396, 1411
BstBAI YACGTR 1 cut(s) 744
BstC8I GCNNGC 5 cut(s) 436, 988, 1088, 1242, 1366
BstDEI CTNAG 3 cut(s) 889, 933, 1472
BstF5I GGATG 4 cut(s) 235, 312, 668, 1348
BstFNI CGCG 1 cut(s) 838
BstKTI GATC 7 cut(s) 76, 358, 596, 622, 802, 1035, 1164
BstMAI GTCTC 5 cut(s) 492, 835, 951, 1117, 1283
BstMBI GATC 7 cut(s) 73, 355, 593, 619, 799, 1032, 1161
BstMWI GCNNNNNNNGC 1 cut(s) 1303
BstNI CCWGG 3 cut(s) 321, 347, 918
BstNSI RCATGY 1 cut(s) 1244
BstPAI GACNNNNGTC 1 cut(s) 1232
BstSCI CCNGG 5 cut(s) 165, 166, 319, 345, 916
BstSFI CTRYAG 3 cut(s) 405, 880, 1385
BstUI CGCG 1 cut(s) 838
BstV1I GCAGC 3 cut(s) 274, 391, 690
BstX2I RGATCY 2 cut(s) 73, 1161
BstYI RGATCY 2 cut(s) 73, 1161
BsuRI GGCC 4 cut(s) 172, 588, 924, 1000
BtsCI GGATG 4 cut(s) 235, 312, 668, 1348
BtsIMutI CAGTG 5 cut(s) 217, 519, 1203, 1392, 1407
Cac8I GCNNGC 5 cut(s) 436, 988, 1088, 1242, 1366
Cfr13I GGNCC 4 cut(s) 170, 908, 923, 992
Cfr9I CCCGGG 1 cut(s) 166
CpoI CGGWCCG 1 cut(s) 992
CsiI ACCWGGT 1 cut(s) 916
Csp6I GTAC 2 cut(s) 745, 1047
CspI CGGWCCG 1 cut(s) 992
CviAII CATG 5 cut(s) 279, 1237, 1241, 1302, 1453
CviQI GTAC 2 cut(s) 745, 1047
DdeI CTNAG 3 cut(s) 889, 933, 1472
DpnI GATC 7 cut(s) 75, 357, 595, 621, 801, 1034, 1163
DpnII GATC 7 cut(s) 73, 355, 593, 619, 799, 1032, 1161
DraIII CACNNNGTG 1 cut(s) 920
DrdI GACNNNNNNGTC 1 cut(s) 1297
DseDI GACNNNNNNGTC 1 cut(s) 1297
EaeI YGGCCR 1 cut(s) 586
EciI GGCGGA 1 cut(s) 988
Eco130I CCWWGG 2 cut(s) 124, 688
Eco147I AGGCCT 1 cut(s) 1000
Eco31I GGTCTC 1 cut(s) 951
Eco32I GATATC 1 cut(s) 115
Eco47I GGWCC 2 cut(s) 908, 992
Eco88I CYCGRG 1 cut(s) 166
EcoRII CCWGG 3 cut(s) 319, 345, 916
EcoRV GATATC 1 cut(s) 115
EcoT14I CCWWGG 2 cut(s) 124, 688
EcoT22I ATGCAT 1 cut(s) 1242
ErhI CCWWGG 2 cut(s) 124, 688
FaeI CATG 5 cut(s) 282, 1240, 1244, 1305, 1456
FaqI GGGAC 2 cut(s) 18, 294
FatI CATG 5 cut(s) 278, 1236, 1240, 1301, 1452
FauI CCCGC 1 cut(s) 886
FbaI TGATCA 3 cut(s) 619, 799, 1032
Fnu4HI GCNGC 3 cut(s) 263, 405, 704
FokI GGATG 4 cut(s) 242, 299, 655, 1355
Fsp4HI GCNGC 3 cut(s) 263, 405, 704
GluI GCNGC 3 cut(s) 263, 405, 704
GsuI CTGGAG 4 cut(s) 329, 495, 516, 1208
HaeIII GGCC 4 cut(s) 172, 588, 924, 1000
HapII CCGG 4 cut(s) 167, 452, 1167, 1283
Hin1II CATG 5 cut(s) 282, 1240, 1244, 1305, 1456
HincII GTYRAC 1 cut(s) 940
HindII GTYRAC 1 cut(s) 940
HinfI GANTC 4 cut(s) 6, 182, 684, 1279
HpaII CCGG 4 cut(s) 167, 452, 1167, 1283
HphI GGTGA 5 cut(s) 94, 122, 181, 325, 475
Hpy166II GTNNAC 3 cut(s) 940, 1356, 1420
Hpy188I TCNGA 3 cut(s) 644, 683, 1253
Hpy188III TCNNGA 6 cut(s) 755, 950, 1159, 1167, 1225, 1283
Hpy8I GTNNAC 3 cut(s) 940, 1356, 1420
Hpy99I CGWCG 1 cut(s) 572
HpyAV CCTTC 7 cut(s) 264, 393, 403, 523, 571, 713, 1126
HpyCH4III ACNGT 5 cut(s) 52, 616, 884, 1396, 1411
HpyCH4IV ACGT 1 cut(s) 743
HpyCH4V TGCA 9 cut(s) 262, 407, 665, 736, 863, 1240, 1297, 1387, 1407
HpyF10VI GCNNNNNNNGC 1 cut(s) 1303
HpyF3I CTNAG 3 cut(s) 889, 933, 1472
HpySE526I ACGT 1 cut(s) 743
Hsp92II CATG 5 cut(s) 282, 1240, 1244, 1305, 1456
Kpn2I TCCGGA 2 cut(s) 1166, 1282
Ksp22I TGATCA 3 cut(s) 619, 799, 1032
Kzo9I GATC 7 cut(s) 73, 355, 593, 619, 799, 1032, 1161
LmnI GCTCC 1 cut(s) 1075
Lsp1109I GCAGC 3 cut(s) 274, 391, 690
LweI GCATC 3 cut(s) 432, 690, 1197
MabI ACCWGGT 1 cut(s) 916
MaeII ACGT 1 cut(s) 743
MaeIII GTNAC 6 cut(s) 100, 187, 368, 692, 790, 884
MalI GATC 7 cut(s) 75, 357, 595, 621, 801, 1034, 1163
MboI GATC 7 cut(s) 73, 355, 593, 619, 799, 1032, 1161
MboII GAAGA 3 cut(s) 83, 548, 866
MflI RGATCY 2 cut(s) 73, 1161
MhlI GDGCHC 1 cut(s) 858
MluCI AATT 8 cut(s) 161, 304, 360, 534, 660, 676, 976, 1464
MluI ACGCGT 1 cut(s) 836
MmeI TCCRAC 2 cut(s) 637, 1147
Mph1103I ATGCAT 1 cut(s) 1242
MroI TCCGGA 2 cut(s) 1166, 1282
MroXI GAANNNNTTC 3 cut(s) 237, 723, 901
MseI TTAA 3 cut(s) 1076, 1140, 1373
MslI CAYNNNNRTG 1 cut(s) 1311
MspA1I CMGCKG 2 cut(s) 797, 893
MspI CCGG 4 cut(s) 167, 452, 1167, 1283
MspR9I CCNGG 5 cut(s) 167, 168, 321, 347, 918
Mva1269I GAATGC 1 cut(s) 723
MvaI CCWGG 3 cut(s) 321, 347, 918
MvnI CGCG 1 cut(s) 838
MwoI GCNNNNNNNGC 1 cut(s) 1303
NciI CCSGG 2 cut(s) 167, 168
NdeII GATC 7 cut(s) 73, 355, 593, 619, 799, 1032, 1161
NlaIII CATG 5 cut(s) 282, 1240, 1244, 1305, 1456
NlaIV GGNNCC 2 cut(s) 171, 456
NmeAIII GCCGAG 1 cut(s) 614
NmuCI GTSAC 4 cut(s) 100, 187, 692, 790
NsiI ATGCAT 1 cut(s) 1242
NspI RCATGY 1 cut(s) 1244
PaeI GCATGC 1 cut(s) 1244
PceI AGGCCT 1 cut(s) 1000
PctI GAATGC 1 cut(s) 723
PdmI GAANNNNTTC 3 cut(s) 237, 723, 901
PfeI GAWTC 3 cut(s) 182, 684, 1279
PflMI CCANNNNNTGG 1 cut(s) 920
PkrI GCNGC 3 cut(s) 264, 406, 705
Ppu21I YACGTR 1 cut(s) 744
PshAI GACNNNNGTC 1 cut(s) 1232
Psp6I CCWGG 3 cut(s) 319, 345, 916
PspGI CCWGG 3 cut(s) 319, 345, 916
PspN4I GGNNCC 2 cut(s) 171, 456
PspPI GGNCC 4 cut(s) 170, 908, 923, 992
PstI CTGCAG 2 cut(s) 409, 1389
PsuI RGATCY 2 cut(s) 73, 1161
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 2 cut(s) 746, 1048
RsaNI GTAC 2 cut(s) 745, 1047
RseI CAYNNNNRTG 1 cut(s) 1311
Rsr2I CGGWCCG 1 cut(s) 992
RsrII CGGWCCG 1 cut(s) 992
SaqAI TTAA 3 cut(s) 1076, 1140, 1373
SatI GCNGC 3 cut(s) 263, 405, 704
Sau3AI GATC 7 cut(s) 73, 355, 593, 619, 799, 1032, 1161
Sau96I GGNCC 4 cut(s) 170, 908, 923, 992
ScrFI CCNGG 5 cut(s) 167, 168, 321, 347, 918
SduI GDGCHC 1 cut(s) 858
SexAI ACCWGGT 1 cut(s) 916
SfaNI GCATC 3 cut(s) 432, 690, 1197
SfcI CTRYAG 3 cut(s) 405, 880, 1385
SinI GGWCC 2 cut(s) 908, 992
SmaI CCCGGG 1 cut(s) 168
SmiMI CAYNNNNRTG 1 cut(s) 1311
SmlI CTYRAG 1 cut(s) 1008
SmoI CTYRAG 1 cut(s) 1008
SphI GCATGC 1 cut(s) 1244
Sse9I AATT 8 cut(s) 161, 304, 360, 534, 660, 676, 976, 1464
SseBI AGGCCT 1 cut(s) 1000
SsiI CCGC 4 cut(s) 809, 893, 973, 990
SspI AATATT 2 cut(s) 1432, 1443
StuI AGGCCT 1 cut(s) 1000
StyD4I CCNGG 5 cut(s) 165, 166, 319, 345, 916
StyI CCWWGG 2 cut(s) 124, 688
TaaI ACNGT 5 cut(s) 52, 616, 884, 1396, 1411
TaiI ACGT 1 cut(s) 746
TaqII GACCGA 2 cut(s) 951, 1009
TasI AATT 8 cut(s) 161, 304, 360, 534, 660, 676, 976, 1464
TfiI GAWTC 3 cut(s) 182, 684, 1279
Tru1I TTAA 3 cut(s) 1076, 1140, 1373
Tru9I TTAA 3 cut(s) 1076, 1140, 1373
TscAI CASTG 5 cut(s) 217, 526, 1210, 1399, 1414
TseFI GTSAC 4 cut(s) 100, 187, 692, 790
TseI GCWGC 3 cut(s) 262, 404, 703
Tsp45I GTSAC 4 cut(s) 100, 187, 692, 790
TspDTI ATGAA 2 cut(s) 246, 752
TspGWI ACGGA 2 cut(s) 585, 1062
TspMI CCCGGG 1 cut(s) 166
TspRI CASTG 5 cut(s) 217, 526, 1210, 1399, 1414
Van91I CCANNNNNTGG 1 cut(s) 920
VpaK11BI GGWCC 2 cut(s) 908, 992
XapI RAATTY 2 cut(s) 976, 1464
XceI RCATGY 1 cut(s) 1244
XmaI CCCGGG 1 cut(s) 166
XmnI GAANNNNTTC 3 cut(s) 237, 723, 901
Zsp2I ATGCAT 1 cut(s) 1242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.