Rh2AG539900

L-ascorbate oxidase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013817
Physical Location & Seq
Reverse (-)
76761477 .. 76766051
4575 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG539900.1

Sequence Viewer

Length: 1398 bp
ATGCTGCAATTGTTGTGTAGGAATGGTGTGCAGCAGAGAAGGAACTCATGGCAAGATGGGGTCTTTGGCACAAATTGTCCCATCCCACCAGGACAGAACTTCACCTATGTTCTCCAGGTCAAGGATCAAATTGGAAGTTACTTTTACTTTCCTTCCCTTGCCTTCCACAAAGCTGCAGGAGGATTTGGTGGCATCAAAATAGCAAGCCGACCTCGTATTCCGGTGCCTTTTCCTCCTCCTGCTGGAGATTTCACCATTCTGGCCGGAGACTGGTTCAAGCAAAATCACACTGATTTGAAGGCAATTTTAGATGGTGGGAATAATCTTCCCTTCCCTGACGGACTTCTCATCAATGGCCGAGGATCAAATGGCAATACATATACAGTTGATCAAGGCAACACTTACAGGTTCAGAATATCAAATGTTGGAATTGCAACATCCATCAATTTCAGAATCCAAGGTCACAAGATGCTGCTGGTTGAGGTGGAAGGCATTCACACCTTGCAAAACACGTACAGCTCTCTTGACATTCATTTGGGGCAGTCCTACTCTGTTCTGGTCACAGCTGATCAACCACCACAAGATTACTACATTGTTGTCTCTACGCGTTTCACTTCACAAGTGCTCTCTGCAACCTCCATTCTTCACTACAGTAACTCAGCGGGAAGTGTTTCTGGTCCTCCACCTGGTGGCCCAACTACTCAGGTTGACTGGTCTCTTGAACAGGCTCGGTCTTTCAGGCGGAATTTGACAGCAAGCGGACCGAGGCCTAACCCTCAAGGCTCATATCATTATGGATTGATCAACACCACCCGTACAATCAGGCTTACAAACTCTGCTCCGATAATCAATGGCAAGCAAAGATATGCTGTAAATAGTGTCTCCTTCCTCCAACCTGACACACCACTTAAAGTTGCTGACTACTTCAAGATCTCCGGAGTATTCTCCATTGGAAGCATCACAGATAGCCCCACTGGTGGTGGTGCTTATCTCCAGACTTCTGTCATGCATGCTGATTTCCGAGCCTTTGTTGAACTCGTGTTTGAGAATCCGGAGGAGACTTTGCAGTCATGGCACATTGATGGTTATAGTTTCTTTGTTGTTGGTATGGATGGTGGAGAGTGGACACCAGCAAGCAGATTAAGATACAATCTGCAGGACACTGTTTCTCGTTGCACTGTTCAGGTGTATCCCAACTCATGGACTGCAATTTACGTGCCTTTGGACAATGTGGGAATGTGGAATGTGAGATCTGAGAATTGGGCACGACAGTACTTAGGCCAGCAGTTCTATCTTCGTGTATACTCACCAGCGAATTCATGGAGAGATGAATATCCAATTCCAAAGAATGCACTTCTGTGTGGTCGGGCATCAGGCCGGCGAACACGGCCTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

465

Amino Acids

51.66

Weight (kDa)

9.2

Isoelectric Point (pI)

43.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 8 - 70 2.9e-19 Multicopper oxidase
Cu-oxidase PF00394 83 - 220 3.1e-42 Multicopper oxidase
Cu-oxidase_2 PF07731 300 - 437 1.1e-31 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013817)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g42990 FvH4_6g42990
malus_domestica MD09G1110500.v1.1 MD17G1098300.v1.1
prunus_persica Prupe.3G216000_v2.0.a1
pyrus_communis pycom09g03290 pycom17g09370
rosa_chinensis RchiOBHm_Chr2g0158971
rosa_laevigata RLG00000021129
rosa_multiflora Rmu_sc0007907.1_g000005
rosa_roxburghii Rroxscaffold_2G00090830
rosa_rugosa Rorug02G0473300
rosa_samantha Rh2AG539900 Rh2BG552700 Rh2CG523200 Rh2DG562500
rosa_wichuraiana Rw2G044690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1066
AccB1I GGYRCC 1 cut(s) 223
AccB7I CCANNNNNTGG 2 cut(s) 689, 1200
AccI GTMKAC 1 cut(s) 1302
AccII CGCG 1 cut(s) 607
AccIII TCCGGA 2 cut(s) 935, 1051
AciI CCGC 3 cut(s) 662, 742, 759
AclWI GGATC 2 cut(s) 132, 370
AcoI YGGCCR 2 cut(s) 261, 355
AcsI RAATTY 2 cut(s) 745, 1315
AdeI CACNNNGTG 1 cut(s) 689
AfaI GTAC 3 cut(s) 515, 817, 1274
AfiI CCNNNNNNNGG 7 cut(s) 121, 242, 270, 686, 689, 977, 1200
AflIII ACRYGT 2 cut(s) 510, 605
AgsI TTSAA 5 cut(s) 277, 298, 722, 928, 1034
AjnI CCWGG 3 cut(s) 88, 114, 685
AleI CACNNNNGTG 1 cut(s) 1357
AluBI AGCT 3 cut(s) 173, 519, 566
AluI AGCT 3 cut(s) 173, 519, 566
Alw21I GWGCWC 1 cut(s) 627
Alw26I GTCTC 5 cut(s) 261, 604, 720, 886, 1052
AlwI GGATC 2 cut(s) 132, 370
Aor13HI TCCGGA 2 cut(s) 935, 1051
AoxI GGCC 7 cut(s) 261, 355, 691, 767, 1279, 1375, 1388
ApeKI GCWGC 4 cut(s) 4, 31, 173, 472
ApoI RAATTY 2 cut(s) 745, 1315
Asp700I GAANNNNTTC 2 cut(s) 492, 670
AspS9I GGNCC 3 cut(s) 677, 692, 761
AsuHPI GGTGA 3 cut(s) 94, 244, 1299
AvaII GGWCC 2 cut(s) 677, 761
BaeGI GKGCMC 1 cut(s) 1267
BanI GGYRCC 1 cut(s) 223
BauI CACGAG 1 cut(s) 1037
Bbv12I GWGCWC 1 cut(s) 627
BbvI GCAGC 3 cut(s) 43, 160, 459
BccI CCATC 6 cut(s) 50, 89, 305, 449, 1076, 1106
BciT130I CCWGG 3 cut(s) 90, 116, 687
BciVI GTATCC 1 cut(s) 1200
BclI TGATCA 3 cut(s) 388, 568, 801
BcoDI GTCTC 5 cut(s) 261, 604, 720, 886, 1052
BfmI CTRYAG 3 cut(s) 174, 649, 1154
BfuI GTATCC 1 cut(s) 1200
BglII AGATCT 2 cut(s) 930, 1250
BisI GCNGC 4 cut(s) 5, 32, 174, 473
BlsI GCNGC 4 cut(s) 6, 33, 175, 474
BmcAI AGTACT 1 cut(s) 1274
Bme1390I CCNGG 3 cut(s) 90, 116, 687
Bme18I GGWCC 2 cut(s) 677, 761
BmgT120I GGNCC 3 cut(s) 677, 692, 761
BmiI GGNNCC 1 cut(s) 225
BmrFI CCNGG 3 cut(s) 90, 116, 687
BmsI GCATC 4 cut(s) 201, 459, 966, 1379
BoxI GACNNNNGTC 1 cut(s) 1001
BpmI CTGGAG 3 cut(s) 98, 264, 977
BpuEI CTTGAG 1 cut(s) 762
BsaAI YACGTR 2 cut(s) 513, 1216
BsaBI GATNNNNATC 1 cut(s) 1332
BsaI GGTCTC 1 cut(s) 720
BsaJI CCNNGG 3 cut(s) 358, 457, 764
BsaWI WCCGGW 3 cut(s) 220, 935, 1051
Bsc4I CCNNNNNNNGG 7 cut(s) 121, 242, 270, 686, 689, 977, 1200
Bse118I RCCGGY 1 cut(s) 1377
Bse1I ACTGG 3 cut(s) 275, 716, 979
Bse8I GATNNNNATC 1 cut(s) 1332
BseAI TCCGGA 2 cut(s) 935, 1051
BseBI CCWGG 3 cut(s) 90, 116, 687
BseDI CCNNGG 3 cut(s) 358, 457, 764
BseGI GGATG 3 cut(s) 81, 437, 1117
BseJI GATNNNNATC 1 cut(s) 1332
BseLI CCNNNNNNNGG 7 cut(s) 121, 242, 270, 686, 689, 977, 1200
BseMII CTCAG 3 cut(s) 672, 716, 1245
BseNI ACTGG 3 cut(s) 275, 716, 979
BseRI GAGGAG 2 cut(s) 225, 1070
BseSI GKGCMC 1 cut(s) 1267
BseXI GCAGC 3 cut(s) 43, 160, 459
BsgI GTGCAG 1 cut(s) 50
Bsh1236I CGCG 1 cut(s) 607
BshFI GGCC 7 cut(s) 263, 357, 693, 769, 1281, 1377, 1390
BshNI GGYRCC 1 cut(s) 223
BsiHKAI GWGCWC 1 cut(s) 627
BsiSI CCGG 5 cut(s) 221, 264, 936, 1052, 1378
BslFI GGGAC 1 cut(s) 63
BslI CCNNNNNNNGG 7 cut(s) 121, 242, 270, 686, 689, 977, 1200
BsmAI GTCTC 5 cut(s) 261, 604, 720, 886, 1052
BsmFI GGGAC 1 cut(s) 63
BsmI GAATGC 2 cut(s) 492, 1354
BsnI GGCC 7 cut(s) 263, 357, 693, 769, 1281, 1377, 1390
Bso31I GGTCTC 1 cut(s) 720
Bsp1286I GDGCHC 2 cut(s) 627, 1267
Bsp13I TCCGGA 2 cut(s) 935, 1051
Bsp143I GATC 7 cut(s) 124, 362, 388, 568, 801, 930, 1250
BspACI CCGC 3 cut(s) 662, 742, 759
BspANI GGCC 7 cut(s) 263, 357, 693, 769, 1281, 1377, 1390
BspCNI CTCAG 3 cut(s) 671, 715, 1246
BspEI TCCGGA 2 cut(s) 935, 1051
BspFNI CGCG 1 cut(s) 607
BspLI GGNNCC 1 cut(s) 225
BspMAI CTGCAG 2 cut(s) 178, 1158
BspPI GGATC 2 cut(s) 132, 370
BspT107I GGYRCC 1 cut(s) 223
BspTNI GGTCTC 1 cut(s) 720
BsrFI RCCGGY 1 cut(s) 1377
BsrI ACTGG 3 cut(s) 275, 716, 979
BssAI RCCGGY 1 cut(s) 1377
BssECI CCNNGG 3 cut(s) 358, 457, 764
BssMI GATC 7 cut(s) 124, 362, 388, 568, 801, 930, 1250
BssNAI GTATAC 1 cut(s) 1303
BssSI CACGAG 1 cut(s) 1037
BssT1I CCWWGG 1 cut(s) 457
Bst1107I GTATAC 1 cut(s) 1303
Bst2BI CACGAG 1 cut(s) 1037
Bst2UI CCWGG 3 cut(s) 90, 116, 687
Bst4CI ACNGT 5 cut(s) 385, 653, 1165, 1180, 1272
BstBAI YACGTR 2 cut(s) 513, 1216
BstC8I GCNNGC 7 cut(s) 205, 757, 857, 1011, 1135, 1283, 1379
BstDEI CTNAG 4 cut(s) 658, 702, 1254, 1276
BstF5I GGATG 3 cut(s) 81, 437, 1117
BstFNI CGCG 1 cut(s) 607
BstKTI GATC 7 cut(s) 127, 365, 391, 571, 804, 933, 1253
BstMAI GTCTC 5 cut(s) 261, 604, 720, 886, 1052
BstMBI GATC 7 cut(s) 124, 362, 388, 568, 801, 930, 1250
BstMWI GCNNNNNNNGC 2 cut(s) 1072, 1387
BstNI CCWGG 3 cut(s) 90, 116, 687
BstNSI RCATGY 1 cut(s) 1013
BstPAI GACNNNNGTC 1 cut(s) 1001
BstSCI CCNGG 3 cut(s) 88, 114, 685
BstSFI CTRYAG 3 cut(s) 174, 649, 1154
BstSLI GKGCMC 1 cut(s) 1267
BstUI CGCG 1 cut(s) 607
BstV1I GCAGC 3 cut(s) 43, 160, 459
BstX2I RGATCY 2 cut(s) 930, 1250
BstYI RGATCY 2 cut(s) 930, 1250
BstZ17I GTATAC 1 cut(s) 1303
BsuI GTATCC 1 cut(s) 1200
BsuRI GGCC 7 cut(s) 263, 357, 693, 769, 1281, 1377, 1390
BtsCI GGATG 3 cut(s) 81, 437, 1117
BtsIMutI CAGTG 4 cut(s) 288, 972, 1161, 1176
Cac8I GCNNGC 7 cut(s) 205, 757, 857, 1011, 1135, 1283, 1379
Cfr10I RCCGGY 1 cut(s) 1377
Cfr13I GGNCC 3 cut(s) 677, 692, 761
CpoI CGGWCCG 1 cut(s) 761
CsiI ACCWGGT 1 cut(s) 685
Csp6I GTAC 3 cut(s) 514, 816, 1273
CspI CGGWCCG 1 cut(s) 761
CviAII CATG 6 cut(s) 48, 1006, 1010, 1071, 1200, 1320
CviQI GTAC 3 cut(s) 514, 816, 1273
DdeI CTNAG 4 cut(s) 658, 702, 1254, 1276
DpnI GATC 7 cut(s) 126, 364, 390, 570, 803, 932, 1252
DpnII GATC 7 cut(s) 124, 362, 388, 568, 801, 930, 1250
DraIII CACNNNGTG 1 cut(s) 689
DrdI GACNNNNNNGTC 1 cut(s) 1066
DseDI GACNNNNNNGTC 1 cut(s) 1066
EaeI YGGCCR 2 cut(s) 261, 355
EciI GGCGGA 1 cut(s) 757
Eco130I CCWWGG 1 cut(s) 457
Eco147I AGGCCT 1 cut(s) 769
Eco31I GGTCTC 1 cut(s) 720
Eco47I GGWCC 2 cut(s) 677, 761
EcoRI GAATTC 1 cut(s) 1315
EcoRII CCWGG 3 cut(s) 88, 114, 685
EcoT14I CCWWGG 1 cut(s) 457
EcoT22I ATGCAT 1 cut(s) 1011
ErhI CCWWGG 1 cut(s) 457
FaeI CATG 6 cut(s) 51, 1009, 1013, 1074, 1203, 1323
FaqI GGGAC 1 cut(s) 63
FatI CATG 6 cut(s) 47, 1005, 1009, 1070, 1199, 1319
FauI CCCGC 1 cut(s) 655
FbaI TGATCA 3 cut(s) 388, 568, 801
FblI GTMKAC 1 cut(s) 1302
Fnu4HI GCNGC 4 cut(s) 5, 32, 174, 473
FokI GGATG 3 cut(s) 68, 424, 1124
Fsp4HI GCNGC 4 cut(s) 5, 32, 174, 473
GluI GCNGC 4 cut(s) 5, 32, 174, 473
GsuI CTGGAG 3 cut(s) 98, 264, 977
HaeIII GGCC 7 cut(s) 263, 357, 693, 769, 1281, 1377, 1390
HapII CCGG 5 cut(s) 221, 264, 936, 1052, 1378
Hin1II CATG 6 cut(s) 51, 1009, 1013, 1074, 1203, 1323
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HinfI GANTC 2 cut(s) 453, 1048
HpaII CCGG 5 cut(s) 221, 264, 936, 1052, 1378
HphI GGTGA 3 cut(s) 94, 244, 1299
Hpy166II GTNNAC 3 cut(s) 709, 1125, 1303
Hpy188I TCNGA 5 cut(s) 413, 452, 843, 1022, 1255
Hpy188III TCNNGA 6 cut(s) 524, 719, 928, 936, 994, 1052
Hpy8I GTNNAC 3 cut(s) 709, 1125, 1303
HpyAV CCTTC 7 cut(s) 33, 162, 172, 292, 340, 482, 895
HpyCH4III ACNGT 5 cut(s) 385, 653, 1165, 1180, 1272
HpyCH4IV ACGT 2 cut(s) 512, 1215
HpyF10VI GCNNNNNNNGC 2 cut(s) 1072, 1387
HpyF3I CTNAG 4 cut(s) 658, 702, 1254, 1276
HpySE526I ACGT 2 cut(s) 512, 1215
Hsp92II CATG 6 cut(s) 51, 1009, 1013, 1074, 1203, 1323
Kpn2I TCCGGA 2 cut(s) 935, 1051
KroI GCCGGC 1 cut(s) 1377
KroNI GCCGGC 1 cut(s) 1379
Ksp22I TGATCA 3 cut(s) 388, 568, 801
Kzo9I GATC 7 cut(s) 124, 362, 388, 568, 801, 930, 1250
LmnI GCTCC 1 cut(s) 844
Lsp1109I GCAGC 3 cut(s) 43, 160, 459
LweI GCATC 4 cut(s) 201, 459, 966, 1379
MabI ACCWGGT 1 cut(s) 685
MaeII ACGT 2 cut(s) 512, 1215
MaeIII GTNAC 4 cut(s) 137, 461, 559, 653
MalI GATC 7 cut(s) 126, 364, 390, 570, 803, 932, 1252
MboI GATC 7 cut(s) 124, 362, 388, 568, 801, 930, 1250
MboII GAAGA 3 cut(s) 317, 635, 1286
MfeI CAATTG 1 cut(s) 8
MflI RGATCY 2 cut(s) 930, 1250
MhlI GDGCHC 2 cut(s) 627, 1267
MluI ACGCGT 1 cut(s) 605
MmeI TCCRAC 2 cut(s) 406, 916
Mph1103I ATGCAT 1 cut(s) 1011
MroI TCCGGA 2 cut(s) 935, 1051
MroNI GCCGGC 1 cut(s) 1377
MroXI GAANNNNTTC 2 cut(s) 492, 670
MseI TTAA 2 cut(s) 909, 1142
MslI CAYNNNNRTG 2 cut(s) 1080, 1357
MspA1I CMGCKG 2 cut(s) 566, 662
MspI CCGG 5 cut(s) 221, 264, 936, 1052, 1378
MspR9I CCNGG 3 cut(s) 90, 116, 687
MunI CAATTG 1 cut(s) 8
Mva1269I GAATGC 2 cut(s) 492, 1354
MvaI CCWGG 3 cut(s) 90, 116, 687
MvnI CGCG 1 cut(s) 607
MwoI GCNNNNNNNGC 2 cut(s) 1072, 1387
NaeI GCCGGC 1 cut(s) 1379
NdeII GATC 7 cut(s) 124, 362, 388, 568, 801, 930, 1250
NgoMIV GCCGGC 1 cut(s) 1377
NlaIII CATG 6 cut(s) 51, 1009, 1013, 1074, 1203, 1323
NlaIV GGNNCC 1 cut(s) 225
NmeAIII GCCGAG 1 cut(s) 383
NmuCI GTSAC 2 cut(s) 461, 559
NsiI ATGCAT 1 cut(s) 1011
NspI RCATGY 1 cut(s) 1013
OliI CACNNNNGTG 1 cut(s) 1357
PaeI GCATGC 1 cut(s) 1013
PceI AGGCCT 1 cut(s) 769
PctI GAATGC 2 cut(s) 492, 1354
PdiI GCCGGC 1 cut(s) 1379
PdmI GAANNNNTTC 2 cut(s) 492, 670
PfeI GAWTC 2 cut(s) 453, 1048
PflMI CCANNNNNTGG 2 cut(s) 689, 1200
PkrI GCNGC 4 cut(s) 6, 33, 175, 474
Ppu21I YACGTR 2 cut(s) 513, 1216
PshAI GACNNNNGTC 1 cut(s) 1001
Psp6I CCWGG 3 cut(s) 88, 114, 685
PspGI CCWGG 3 cut(s) 88, 114, 685
PspN4I GGNNCC 1 cut(s) 225
PspPI GGNCC 3 cut(s) 677, 692, 761
PstI CTGCAG 2 cut(s) 178, 1158
PsuI RGATCY 2 cut(s) 930, 1250
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 3 cut(s) 515, 817, 1274
RsaNI GTAC 3 cut(s) 514, 816, 1273
RseI CAYNNNNRTG 2 cut(s) 1080, 1357
Rsr2I CGGWCCG 1 cut(s) 761
RsrII CGGWCCG 1 cut(s) 761
SaqAI TTAA 2 cut(s) 909, 1142
SatI GCNGC 4 cut(s) 5, 32, 174, 473
Sau3AI GATC 7 cut(s) 124, 362, 388, 568, 801, 930, 1250
Sau96I GGNCC 3 cut(s) 677, 692, 761
ScaI AGTACT 1 cut(s) 1274
ScrFI CCNGG 3 cut(s) 90, 116, 687
SduI GDGCHC 2 cut(s) 627, 1267
SexAI ACCWGGT 1 cut(s) 685
SfaNI GCATC 4 cut(s) 201, 459, 966, 1379
SfcI CTRYAG 3 cut(s) 174, 649, 1154
SinI GGWCC 2 cut(s) 677, 761
SmiMI CAYNNNNRTG 2 cut(s) 1080, 1357
SmlI CTYRAG 1 cut(s) 777
SmoI CTYRAG 1 cut(s) 777
SphI GCATGC 1 cut(s) 1013
SseBI AGGCCT 1 cut(s) 769
SsiI CCGC 3 cut(s) 662, 742, 759
StuI AGGCCT 1 cut(s) 769
StyD4I CCNGG 3 cut(s) 88, 114, 685
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 5 cut(s) 385, 653, 1165, 1180, 1272
TaiI ACGT 2 cut(s) 515, 1218
TaqII GACCGA 2 cut(s) 720, 778
TatI WGTACW 1 cut(s) 1272
TfiI GAWTC 2 cut(s) 453, 1048
Tru1I TTAA 2 cut(s) 909, 1142
Tru9I TTAA 2 cut(s) 909, 1142
TscAI CASTG 4 cut(s) 295, 979, 1168, 1183
TseFI GTSAC 2 cut(s) 461, 559
TseI GCWGC 4 cut(s) 4, 31, 173, 472
Tsp45I GTSAC 2 cut(s) 461, 559
TspDTI ATGAA 3 cut(s) 521, 1308, 1344
TspGWI ACGGA 1 cut(s) 354
TspRI CASTG 4 cut(s) 295, 979, 1168, 1183
Van91I CCANNNNNTGG 2 cut(s) 689, 1200
VpaK11BI GGWCC 2 cut(s) 677, 761
XapI RAATTY 2 cut(s) 745, 1315
XceI RCATGY 1 cut(s) 1013
XcmI CCANNNNNNNNNTGG 1 cut(s) 1317
XmiI GTMKAC 1 cut(s) 1302
XmnI GAANNNNTTC 2 cut(s) 492, 670
ZrmI AGTACT 1 cut(s) 1274
Zsp2I ATGCAT 1 cut(s) 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.