FvH4_6g47020

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011227
Physical Location & Seq
Forward (+)
35741560 .. 35743839
2280 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g47020.t1

Sequence Viewer

Length: 750 bp
ATGAAATGTGATGAGGTTCTGAAGGCTGTTTTTCCTTATCTGGAGGGTGAGGATCTCGCGTCATGTATGGGAGTGTGCAAGCAGTGGAGAAACCTAGCTCAAGATGATTTCTTTTGGAAATGCTTGTGTGCTAAGAGATGGCCTTCTATCTGCAAGAAACCTAGCCCCCCGACTGTAACTTACTACAAGCTGTATCAAACGTTTTATAAGCGCAAGCAGCATCGAACTCTTCTTCCCCCAAGACTTTCCTTTGATGACTTGGAGTTTTTCATTGATATCTGGACTGAAGATGGATTTATCTTTTCAGAAATGGTTCCAGGACCTATCCTACAGAGCGGTATTAAAATCCCACCACCAGGAGCATGTGACATGCTTAAGTTTCACCTGGAGGGTCCAGAGTACAGGATGAAACTCCCTGTTGACCCAAGATTTACTGTTCCTCTGAGCCAGACAGTGAGTGTTTCAGTCCTTGTGGGGCGCAAGGATTCTAATAGGGTTGCTTGCATCATCAATAGATCGATGTTTGATTATATTGATCGAACAGCATACCGAGCCATGGCAAATGACTACCTTGACTTCTCACCTGTCTACCCCTTTGTTTCGGGTATCCGGGCATGGATCTCGTTGCTTTTCATGGAAGAAGGAAATGAAGGTGCCATTGATGTTTTTGGGATTGAAATGGATTTCTGTGATGCAGCCAACTCCAAGGAAGAGGTTTTGTGGCTTTTAGATATGCTTGATTGGAAGTGA

Protein Analysis

250

Amino Acids

28.86

Weight (kDa)

5.22

Isoelectric Point (pI)

52.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 4 - 44 1.6e-11 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
AccB1I GGYRCC 1 cut(s) 653
AccBSI CCGCTC 1 cut(s) 336
AccI GTMKAC 1 cut(s) 588
AccII CGCG 1 cut(s) 59
AciI CCGC 1 cut(s) 336
AclI AACGTT 1 cut(s) 200
AclWI GGATC 2 cut(s) 60, 626
AcuI CTGAAG 2 cut(s) 41, 306
AfaI GTAC 1 cut(s) 401
AfiI CCNNNNNNNGG 2 cut(s) 356, 556
AflII CTTAAG 1 cut(s) 374
AgsI TTSAA 1 cut(s) 677
AjnI CCWGG 3 cut(s) 316, 355, 384
AloI GAACNNNNNNTCC 2 cut(s) 217, 249
AluBI AGCT 2 cut(s) 98, 190
AluI AGCT 2 cut(s) 98, 190
AlwI GGATC 2 cut(s) 60, 626
AoxI GGCC 1 cut(s) 140
ApeKI GCWGC 2 cut(s) 217, 695
Asp700I GAANNNNTTC 1 cut(s) 312
AspLEI GCGC 2 cut(s) 213, 480
AspS9I GGNCC 2 cut(s) 320, 392
AsuC2I CCSGG 1 cut(s) 611
AsuHPI GGTGA 3 cut(s) 59, 374, 573
AvaII GGWCC 2 cut(s) 320, 392
BanI GGYRCC 1 cut(s) 653
BbvI GCAGC 2 cut(s) 229, 707
BccI CCATC 2 cut(s) 132, 284
BcgI CGANNNNNNTGC 2 cut(s) 603, 637
BciT130I CCWGG 3 cut(s) 318, 357, 386
BciVI GTATCC 1 cut(s) 617
BcnI CCSGG 1 cut(s) 611
BfaI CTAG 2 cut(s) 95, 162
BfmI CTRYAG 1 cut(s) 329
BfrI CTTAAG 1 cut(s) 374
BfuI GTATCC 1 cut(s) 617
BisI GCNGC 2 cut(s) 218, 696
BlsI GCNGC 2 cut(s) 219, 697
Bme1390I CCNGG 4 cut(s) 318, 357, 386, 611
Bme18I GGWCC 2 cut(s) 320, 392
BmgT120I GGNCC 2 cut(s) 320, 392
BmiI GGNNCC 3 cut(s) 315, 393, 655
BmrFI CCNGG 4 cut(s) 318, 357, 386, 611
BmsI GCATC 3 cut(s) 229, 513, 682
BpmI CTGGAG 2 cut(s) 62, 407
BpuEI CTTGAG 1 cut(s) 84
BpuMI CCSGG 1 cut(s) 611
Bsa29I ATCGAT 1 cut(s) 518
BsaJI CCNNGG 2 cut(s) 555, 705
Bsc4I CCNNNNNNNGG 2 cut(s) 356, 556
BseBI CCWGG 3 cut(s) 318, 357, 386
BseCI ATCGAT 1 cut(s) 518
BseDI CCNNGG 2 cut(s) 555, 705
BseGI GGATG 1 cut(s) 411
BseLI CCNNNNNNNGG 2 cut(s) 356, 556
BseMII CTCAG 1 cut(s) 434
BseXI GCAGC 2 cut(s) 229, 707
Bsh1236I CGCG 1 cut(s) 59
BshFI GGCC 1 cut(s) 142
BshNI GGYRCC 1 cut(s) 653
BshVI ATCGAT 1 cut(s) 518
BsiSI CCGG 1 cut(s) 610
BslI CCNNNNNNNGG 2 cut(s) 356, 556
BsnI GGCC 1 cut(s) 142
Bsp143I GATC 4 cut(s) 52, 515, 535, 618
Bsp19I CCATGG 1 cut(s) 555
BspACI CCGC 1 cut(s) 336
BspANI GGCC 1 cut(s) 142
BspCNI CTCAG 1 cut(s) 435
BspDI ATCGAT 1 cut(s) 518
BspFNI CGCG 1 cut(s) 59
BspLI GGNNCC 3 cut(s) 315, 393, 655
BspPI GGATC 2 cut(s) 60, 626
BspT107I GGYRCC 1 cut(s) 653
BspTI CTTAAG 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 336
BssECI CCNNGG 2 cut(s) 555, 705
BssMI GATC 4 cut(s) 52, 515, 535, 618
BssT1I CCWWGG 2 cut(s) 555, 705
Bst2UI CCWGG 3 cut(s) 318, 357, 386
Bst4CI ACNGT 3 cut(s) 175, 436, 454
Bst6I CTCTTC 2 cut(s) 234, 705
BstAFI CTTAAG 1 cut(s) 374
BstC8I GCNNGC 3 cut(s) 80, 215, 502
BstDEI CTNAG 2 cut(s) 132, 443
BstDSI CCRYGG 1 cut(s) 555
BstF5I GGATG 1 cut(s) 411
BstFNI CGCG 1 cut(s) 59
BstHHI GCGC 2 cut(s) 213, 480
BstKTI GATC 4 cut(s) 55, 518, 538, 621
BstMBI GATC 4 cut(s) 52, 515, 535, 618
BstMWI GCNNNNNNNGC 2 cut(s) 217, 551
BstNI CCWGG 3 cut(s) 318, 357, 386
BstNSI RCATGY 2 cut(s) 366, 373
BstSCI CCNGG 4 cut(s) 316, 355, 384, 609
BstSFI CTRYAG 1 cut(s) 329
BstUI CGCG 1 cut(s) 59
BstV1I GCAGC 2 cut(s) 229, 707
BstX2I RGATCY 2 cut(s) 52, 618
BstYI RGATCY 2 cut(s) 52, 618
Bsu15I ATCGAT 1 cut(s) 518
BsuI GTATCC 1 cut(s) 617
BsuRI GGCC 1 cut(s) 142
BsuTUI ATCGAT 1 cut(s) 518
BtgI CCRYGG 1 cut(s) 555
BtsCI GGATG 1 cut(s) 411
BtsI GCAGTG 1 cut(s) 89
BtsIMutI CAGTG 2 cut(s) 89, 459
Cac8I GCNNGC 3 cut(s) 80, 215, 502
CfoI GCGC 2 cut(s) 213, 480
Cfr13I GGNCC 2 cut(s) 320, 392
ClaI ATCGAT 1 cut(s) 518
CseI GACGC 1 cut(s) 48
Csp6I GTAC 1 cut(s) 400
CviAII CATG 6 cut(s) 63, 363, 370, 556, 615, 634
CviJI RGCY 9 cut(s) 26, 98, 142, 165, 190, 447, 554, 698, 724
CviKI_1 RGCY 9 cut(s) 26, 98, 142, 165, 190, 447, 554, 698, 724
CviQI GTAC 1 cut(s) 400
DdeI CTNAG 2 cut(s) 132, 443
DpnI GATC 4 cut(s) 54, 517, 537, 620
DpnII GATC 4 cut(s) 52, 515, 535, 618
Eam1104I CTCTTC 2 cut(s) 234, 705
EarI CTCTTC 2 cut(s) 234, 705
Eco130I CCWWGG 2 cut(s) 555, 705
Eco32I GATATC 1 cut(s) 277
Eco47I GGWCC 2 cut(s) 320, 392
Eco57I CTGAAG 2 cut(s) 41, 306
EcoO109I RGGNCCY 1 cut(s) 320
EcoRII CCWGG 3 cut(s) 316, 355, 384
EcoRV GATATC 1 cut(s) 277
EcoT14I CCWWGG 2 cut(s) 555, 705
ErhI CCWWGG 2 cut(s) 555, 705
FaeI CATG 6 cut(s) 66, 366, 373, 559, 618, 637
FatI CATG 6 cut(s) 62, 362, 369, 555, 614, 633
FblI GTMKAC 1 cut(s) 588
Fnu4HI GCNGC 2 cut(s) 218, 696
FokI GGATG 1 cut(s) 418
Fsp4HI GCNGC 2 cut(s) 218, 696
FspBI CTAG 2 cut(s) 95, 162
GlaI GCGC 2 cut(s) 212, 479
GluI GCNGC 2 cut(s) 218, 696
GsuI CTGGAG 2 cut(s) 62, 407
HaeIII GGCC 1 cut(s) 142
HapII CCGG 1 cut(s) 610
HgaI GACGC 1 cut(s) 48
HhaI GCGC 2 cut(s) 213, 480
Hin1II CATG 6 cut(s) 66, 366, 373, 559, 618, 637
Hin6I GCGC 2 cut(s) 211, 478
HinP1I GCGC 2 cut(s) 211, 478
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 1 cut(s) 485
HpaII CCGG 1 cut(s) 610
HphI GGTGA 3 cut(s) 59, 374, 573
Hpy166II GTNNAC 2 cut(s) 421, 589
Hpy188I TCNGA 3 cut(s) 21, 307, 444
Hpy188III TCNNGA 4 cut(s) 41, 101, 280, 395
Hpy8I GTNNAC 2 cut(s) 421, 589
HpyAV CCTTC 4 cut(s) 16, 153, 635, 644
HpyCH4III ACNGT 3 cut(s) 175, 436, 454
HpyCH4IV ACGT 1 cut(s) 200
HpyCH4V TGCA 4 cut(s) 78, 153, 504, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 217, 551
HpyF3I CTNAG 2 cut(s) 132, 443
HpySE526I ACGT 1 cut(s) 200
Hsp92II CATG 6 cut(s) 66, 366, 373, 559, 618, 637
HspAI GCGC 2 cut(s) 211, 478
Kzo9I GATC 4 cut(s) 52, 515, 535, 618
LmnI GCTCC 1 cut(s) 359
Lsp1109I GCAGC 2 cut(s) 229, 707
LweI GCATC 3 cut(s) 229, 513, 682
MaeI CTAG 2 cut(s) 95, 162
MaeII ACGT 1 cut(s) 200
MaeIII GTNAC 2 cut(s) 175, 365
MalI GATC 4 cut(s) 54, 517, 537, 620
MbiI CCGCTC 1 cut(s) 336
MboI GATC 4 cut(s) 52, 515, 535, 618
MboII GAAGA 5 cut(s) 221, 224, 299, 650, 722
MflI RGATCY 2 cut(s) 52, 618
MnlI CCTC 6 cut(s) 7, 37, 43, 382, 450, 706
MroXI GAANNNNTTC 1 cut(s) 312
MseI TTAA 2 cut(s) 342, 375
MspCI CTTAAG 1 cut(s) 374
MspI CCGG 1 cut(s) 610
MspR9I CCNGG 4 cut(s) 318, 357, 386, 611
MvaI CCWGG 3 cut(s) 318, 357, 386
MvnI CGCG 1 cut(s) 59
MwoI GCNNNNNNNGC 2 cut(s) 217, 551
NciI CCSGG 1 cut(s) 611
NcoI CCATGG 1 cut(s) 555
NdeII GATC 4 cut(s) 52, 515, 535, 618
NlaIII CATG 6 cut(s) 66, 366, 373, 559, 618, 637
NlaIV GGNNCC 3 cut(s) 315, 393, 655
NmuCI GTSAC 1 cut(s) 365
NspI RCATGY 2 cut(s) 366, 373
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 1 cut(s) 485
PfoI TCCNGGA 1 cut(s) 316
PkrI GCNGC 2 cut(s) 219, 697
PpuMI RGGWCCY 1 cut(s) 320
PsiI TTATAA 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 200
Psp5II RGGWCCY 1 cut(s) 320
Psp6I CCWGG 3 cut(s) 316, 355, 384
PspGI CCWGG 3 cut(s) 316, 355, 384
PspN4I GGNNCC 3 cut(s) 315, 393, 655
PspPI GGNCC 2 cut(s) 320, 392
PspPPI RGGWCCY 1 cut(s) 320
PsuI RGATCY 2 cut(s) 52, 618
RsaI GTAC 1 cut(s) 401
RsaNI GTAC 1 cut(s) 400
SaqAI TTAA 2 cut(s) 342, 375
SatI GCNGC 2 cut(s) 218, 696
Sau3AI GATC 4 cut(s) 52, 515, 535, 618
Sau96I GGNCC 2 cut(s) 320, 392
ScrFI CCNGG 4 cut(s) 318, 357, 386, 611
SfaNI GCATC 3 cut(s) 229, 513, 682
SfcI CTRYAG 1 cut(s) 329
SinI GGWCC 2 cut(s) 320, 392
SmlI CTYRAG 2 cut(s) 99, 374
SmoI CTYRAG 2 cut(s) 99, 374
SsiI CCGC 1 cut(s) 336
SspMI CTAG 2 cut(s) 95, 162
StyD4I CCNGG 4 cut(s) 316, 355, 384, 609
StyI CCWWGG 2 cut(s) 555, 705
TaaI ACNGT 3 cut(s) 175, 436, 454
TaiI ACGT 1 cut(s) 203
TaqI TCGA 3 cut(s) 223, 518, 538
TatI WGTACW 1 cut(s) 399
TfiI GAWTC 1 cut(s) 485
Tru1I TTAA 2 cut(s) 342, 375
Tru9I TTAA 2 cut(s) 342, 375
TscAI CASTG 2 cut(s) 89, 459
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 2 cut(s) 217, 695
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 5 cut(s) 17, 259, 422, 622, 663
TspRI CASTG 2 cut(s) 89, 459
Vha464I CTTAAG 1 cut(s) 374
VpaK11BI GGWCC 2 cut(s) 320, 392
XceI RCATGY 2 cut(s) 366, 373
XmiI GTMKAC 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 2 cut(s) 95, 162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.