pycom111g05420

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0011227
Physical Location & Seq
Reverse (-)
3470491 .. 3471240
750 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g05420.1

Sequence Viewer

Length: 750 bp
ATGGTGAATGAAGAGGTCCTGAAGGCAGTTTTCCCTTTACTGGAGGGTGTGGACCTTGCTTCTTGTATGGGAGTGTGCAAGCGGTGGAGACAGATAGCTCGAGATGATTTCTTTTGGAAGCGTCTATGTGCCAAGAGATGGCCCTCTACCTGCAAGCGACCTAACCCTCCCACTCTAACCTATTGCAAGCTGTATCAAAACTTTTATAAGCGCCACCACCGTCAAACTCTTCTTCCACCAAGACTTTCCTTTGATGACTTGGAATTTTTCATTGATATATGGACTGAAGATCGATTTCTCTTTTCTGAAGTGATCCCTGGCCCCGTTCTACAGAAAGGTACCAAAACCCCACCACCAGAAGTATGTGACATGCTTAGATATCACCTGGAGGGTGCTGAGTACAAGATGAAACTCCCTGTTGAACCAAGGTTTGCGGTTCCTCTGACCCAGACCGTGAGTGTTTCAGTGCTTGTGGAGCGCAAGGATTCTAACAGGGTTGCACGTATAATTGATAATTCTATGTTTGACTACATTGATCGAACAGCATCTAGAGCCATGGCAAATGAATACCTAGACTTCTCAGCGGTCTACCCTTTCACTTCGGGTATCCGGGCATGGATCTCTTTGCTTTTCATGGACAACGGAGATGAAGGAGTTATTGATGTTTTTGGGATCGAAATGGATTTCATGGATGCTGCAAACTCGAAGGAAGAGGTTTTGTGGCTATTAGACATGCTTGATTGGAAGTGA

Protein Analysis

250

Amino Acids

29.11

Weight (kDa)

5.54

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 4 - 44 1.5e-11 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 158
Acc65I GGTACC 1 cut(s) 338
AccB1I GGYRCC 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 138
AccI GTMKAC 1 cut(s) 588
AciI CCGC 3 cut(s) 82, 434, 584
AclWI GGATC 3 cut(s) 307, 626, 680
AcsI RAATTY 1 cut(s) 263
AcuI CTGAAG 3 cut(s) 41, 306, 327
AfaI GTAC 2 cut(s) 340, 401
AfiI CCNNNNNNNGG 2 cut(s) 40, 138
AgsI TTSAA 1 cut(s) 422
AjnI CCWGG 2 cut(s) 316, 384
AluBI AGCT 2 cut(s) 98, 190
AluI AGCT 2 cut(s) 98, 190
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 3 cut(s) 307, 626, 680
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 2 cut(s) 140, 319
ApeKI GCWGC 1 cut(s) 695
ApoI RAATTY 1 cut(s) 263
Asp718I GGTACC 1 cut(s) 338
AspLEI GCGC 2 cut(s) 213, 480
AspS9I GGNCC 4 cut(s) 16, 52, 141, 320
AsuC2I CCSGG 1 cut(s) 611
AsuHPI GGTGA 2 cut(s) 16, 374
AvaI CYCGRG 1 cut(s) 99
AvaII GGWCC 2 cut(s) 16, 52
BanI GGYRCC 1 cut(s) 338
BbvI GCAGC 1 cut(s) 682
BccI CCATC 1 cut(s) 132
BciT130I CCWGG 2 cut(s) 318, 386
BciVI GTATCC 1 cut(s) 617
BcnI CCSGG 1 cut(s) 611
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 2 cut(s) 549, 572
BfmI CTRYAG 1 cut(s) 329
BfoI RGCGCY 1 cut(s) 214
BfuAI ACCTGC 1 cut(s) 158
BfuI GTATCC 1 cut(s) 617
BisI GCNGC 1 cut(s) 696
BlsI GCNGC 1 cut(s) 697
Bme1390I CCNGG 3 cut(s) 318, 386, 611
Bme18I GGWCC 2 cut(s) 16, 52
BmeT110I CYCGRG 1 cut(s) 99
BmgT120I GGNCC 4 cut(s) 16, 52, 141, 320
BmiI GGNNCC 3 cut(s) 322, 340, 438
BmrFI CCNGG 3 cut(s) 318, 386, 611
BmsI GCATC 2 cut(s) 554, 682
BpmI CTGGAG 2 cut(s) 62, 407
BpuMI CCSGG 1 cut(s) 611
Bsa29I ATCGAT 1 cut(s) 292
BsaAI YACGTR 1 cut(s) 503
BsaJI CCNNGG 3 cut(s) 316, 425, 555
Bsc4I CCNNNNNNNGG 2 cut(s) 40, 138
Bse1I ACTGG 1 cut(s) 45
BseBI CCWGG 2 cut(s) 318, 386
BseCI ATCGAT 1 cut(s) 292
BseDI CCNNGG 3 cut(s) 316, 425, 555
BseGI GGATG 1 cut(s) 697
BseLI CCNNNNNNNGG 2 cut(s) 40, 138
BseMII CTCAG 2 cut(s) 387, 594
BseNI ACTGG 1 cut(s) 45
BseXI GCAGC 1 cut(s) 682
BshFI GGCC 2 cut(s) 142, 321
BshNI GGYRCC 1 cut(s) 338
BshVI ATCGAT 1 cut(s) 292
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 1 cut(s) 610
BslI CCNNNNNNNGG 2 cut(s) 40, 138
BsmAI GTCTC 1 cut(s) 82
BsnI GGCC 2 cut(s) 142, 321
BsoBI CYCGRG 1 cut(s) 99
Bsp143I GATC 5 cut(s) 289, 312, 535, 618, 672
Bsp19I CCATGG 1 cut(s) 555
BspACI CCGC 3 cut(s) 82, 434, 584
BspANI GGCC 2 cut(s) 142, 321
BspCNI CTCAG 2 cut(s) 388, 593
BspDI ATCGAT 1 cut(s) 292
BspLI GGNNCC 3 cut(s) 322, 340, 438
BspMI ACCTGC 1 cut(s) 158
BspPI GGATC 3 cut(s) 307, 626, 680
BspT107I GGYRCC 1 cut(s) 338
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 3 cut(s) 316, 425, 555
BssMI GATC 5 cut(s) 289, 312, 535, 618, 672
BssT1I CCWWGG 2 cut(s) 425, 555
Bst2UI CCWGG 2 cut(s) 318, 386
Bst4CI ACNGT 2 cut(s) 221, 454
Bst6I CTCTTC 3 cut(s) 6, 234, 705
BstBAI YACGTR 1 cut(s) 503
BstC8I GCNNGC 3 cut(s) 80, 155, 188
BstDEI CTNAG 3 cut(s) 374, 396, 580
BstDSI CCRYGG 1 cut(s) 555
BstF5I GGATG 1 cut(s) 697
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 2 cut(s) 213, 480
BstKTI GATC 5 cut(s) 292, 315, 538, 621, 675
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 5 cut(s) 289, 312, 535, 618, 672
BstMWI GCNNNNNNNGC 2 cut(s) 475, 551
BstNI CCWGG 2 cut(s) 318, 386
BstNSI RCATGY 2 cut(s) 373, 736
BstSCI CCNGG 3 cut(s) 316, 384, 609
BstSFI CTRYAG 1 cut(s) 329
BstV1I GCAGC 1 cut(s) 682
BstX2I RGATCY 1 cut(s) 618
BstYI RGATCY 1 cut(s) 618
Bsu15I ATCGAT 1 cut(s) 292
BsuI GTATCC 1 cut(s) 617
BsuRI GGCC 2 cut(s) 142, 321
BsuTUI ATCGAT 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 555
BtsCI GGATG 1 cut(s) 697
BtsIMutI CAGTG 1 cut(s) 471
BveI ACCTGC 1 cut(s) 158
Cac8I GCNNGC 3 cut(s) 80, 155, 188
CfoI GCGC 2 cut(s) 213, 480
Cfr13I GGNCC 4 cut(s) 16, 52, 141, 320
ClaI ATCGAT 1 cut(s) 292
CseI GACGC 1 cut(s) 110
Csp6I GTAC 2 cut(s) 339, 400
CviAII CATG 6 cut(s) 370, 556, 615, 634, 688, 733
CviJI RGCY 6 cut(s) 98, 142, 190, 321, 554, 724
CviKI_1 RGCY 6 cut(s) 98, 142, 190, 321, 554, 724
CviQI GTAC 2 cut(s) 339, 400
DdeI CTNAG 3 cut(s) 374, 396, 580
DpnI GATC 5 cut(s) 291, 314, 537, 620, 674
DpnII GATC 5 cut(s) 289, 312, 535, 618, 672
Eam1104I CTCTTC 3 cut(s) 6, 234, 705
EarI CTCTTC 3 cut(s) 6, 234, 705
Eco130I CCWWGG 2 cut(s) 425, 555
Eco32I GATATC 1 cut(s) 380
Eco47I GGWCC 2 cut(s) 16, 52
Eco57I CTGAAG 3 cut(s) 41, 306, 327
Eco88I CYCGRG 1 cut(s) 99
EcoO109I RGGNCCY 1 cut(s) 16
EcoRII CCWGG 2 cut(s) 316, 384
EcoRV GATATC 1 cut(s) 380
EcoT14I CCWWGG 2 cut(s) 425, 555
ErhI CCWWGG 2 cut(s) 425, 555
FaeI CATG 6 cut(s) 373, 559, 618, 637, 691, 736
FatI CATG 6 cut(s) 369, 555, 614, 633, 687, 732
FblI GTMKAC 1 cut(s) 588
Fnu4HI GCNGC 1 cut(s) 696
FokI GGATG 1 cut(s) 704
Fsp4HI GCNGC 1 cut(s) 696
FspBI CTAG 2 cut(s) 549, 572
GlaI GCGC 2 cut(s) 212, 479
GluI GCNGC 1 cut(s) 696
GsuI CTGGAG 2 cut(s) 62, 407
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 2 cut(s) 142, 321
HapII CCGG 1 cut(s) 610
HgaI GACGC 1 cut(s) 110
HhaI GCGC 2 cut(s) 213, 480
Hin1II CATG 6 cut(s) 373, 559, 618, 637, 691, 736
Hin6I GCGC 2 cut(s) 211, 478
HinP1I GCGC 2 cut(s) 211, 478
HinfI GANTC 1 cut(s) 485
HpaII CCGG 1 cut(s) 610
HphI GGTGA 2 cut(s) 16, 374
Hpy166II GTNNAC 2 cut(s) 52, 589
Hpy188I TCNGA 2 cut(s) 307, 444
Hpy188III TCNNGA 3 cut(s) 19, 101, 549
Hpy8I GTNNAC 2 cut(s) 52, 589
HpyAV CCTTC 3 cut(s) 16, 644, 700
HpyCH4III ACNGT 2 cut(s) 221, 454
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 5 cut(s) 78, 153, 186, 500, 698
HpyF10VI GCNNNNNNNGC 2 cut(s) 475, 551
HpyF3I CTNAG 3 cut(s) 374, 396, 580
HpySE526I ACGT 1 cut(s) 502
Hsp92II CATG 6 cut(s) 373, 559, 618, 637, 691, 736
HspAI GCGC 2 cut(s) 211, 478
KpnI GGTACC 1 cut(s) 342
Kzo9I GATC 5 cut(s) 289, 312, 535, 618, 672
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 1 cut(s) 682
LweI GCATC 2 cut(s) 554, 682
MaeI CTAG 2 cut(s) 549, 572
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 1 cut(s) 365
MalI GATC 5 cut(s) 291, 314, 537, 620, 674
MboI GATC 5 cut(s) 289, 312, 535, 618, 672
MboII GAAGA 5 cut(s) 23, 221, 224, 299, 722
MflI RGATCY 1 cut(s) 618
MluCI AATT 3 cut(s) 263, 507, 514
MnlI CCTC 7 cut(s) 7, 37, 154, 177, 382, 450, 706
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 1 cut(s) 610
MspR9I CCNGG 3 cut(s) 318, 386, 611
MvaI CCWGG 2 cut(s) 318, 386
MwoI GCNNNNNNNGC 2 cut(s) 475, 551
NciI CCSGG 1 cut(s) 611
NcoI CCATGG 1 cut(s) 555
NdeII GATC 5 cut(s) 289, 312, 535, 618, 672
NlaIII CATG 6 cut(s) 373, 559, 618, 637, 691, 736
NlaIV GGNNCC 3 cut(s) 322, 340, 438
NmuCI GTSAC 1 cut(s) 365
NspI RCATGY 2 cut(s) 373, 736
PaeR7I CTCGAG 1 cut(s) 99
PfeI GAWTC 1 cut(s) 485
PflMI CCANNNNNTGG 1 cut(s) 138
PkrI GCNGC 1 cut(s) 697
Ppu21I YACGTR 1 cut(s) 503
PpuMI RGGWCCY 1 cut(s) 16
PsiI TTATAA 1 cut(s) 207
Psp5II RGGWCCY 1 cut(s) 16
Psp6I CCWGG 2 cut(s) 316, 384
PspGI CCWGG 2 cut(s) 316, 384
PspN4I GGNNCC 3 cut(s) 322, 340, 438
PspPI GGNCC 4 cut(s) 16, 52, 141, 320
PspPPI RGGWCCY 1 cut(s) 16
PsuI RGATCY 1 cut(s) 618
RsaI GTAC 2 cut(s) 340, 401
RsaNI GTAC 2 cut(s) 339, 400
SatI GCNGC 1 cut(s) 696
Sau3AI GATC 5 cut(s) 289, 312, 535, 618, 672
Sau96I GGNCC 4 cut(s) 16, 52, 141, 320
ScrFI CCNGG 3 cut(s) 318, 386, 611
SfaNI GCATC 2 cut(s) 554, 682
SfcI CTRYAG 1 cut(s) 329
Sfr274I CTCGAG 1 cut(s) 99
SinI GGWCC 2 cut(s) 16, 52
SlaI CTCGAG 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 99
SmoI CTYRAG 1 cut(s) 99
Sse9I AATT 3 cut(s) 263, 507, 514
SsiI CCGC 3 cut(s) 82, 434, 584
SspMI CTAG 2 cut(s) 549, 572
StyD4I CCNGG 3 cut(s) 316, 384, 609
StyI CCWWGG 2 cut(s) 425, 555
TaaI ACNGT 2 cut(s) 221, 454
TaiI ACGT 1 cut(s) 505
TaqI TCGA 5 cut(s) 100, 292, 538, 675, 704
TasI AATT 3 cut(s) 263, 507, 514
TatI WGTACW 1 cut(s) 399
TfiI GAWTC 1 cut(s) 485
TscAI CASTG 1 cut(s) 471
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 1 cut(s) 695
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 7 cut(s) 24, 259, 422, 579, 622, 663, 676
TspGWI ACGGA 1 cut(s) 657
TspRI CASTG 1 cut(s) 471
Van91I CCANNNNNTGG 1 cut(s) 138
VpaK11BI GGWCC 2 cut(s) 16, 52
XapI RAATTY 1 cut(s) 263
XbaI TCTAGA 1 cut(s) 548
XceI RCATGY 2 cut(s) 373, 736
XhoI CTCGAG 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 588
XspI CTAG 2 cut(s) 549, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.