MD09G1064500.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0011227
Physical Location & Seq
Reverse (-)
4415629 .. 4417706
2078 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1064500.v1.1.491

Sequence Viewer

Length: 750 bp
ATGGTGAATGAAGTGGTCCTGAAGGCGGTTTTCCCTTTACTGGAGGGTGTGGACCTTGCTTCTTGTATGGGGGTGTGCAAGCGGTGGAGACAGATAGCTCGAGATGATTTCTTTTGGAAGCGTCTATGTGCCAAGAGATGGCCCTCTACCTGCAAGCGACCTAACCCTCCCACTCTAACCTATTGCAAGCTGTATCAAAACTTTTATAAGCGCCACCACCGTCGAACTCTTCTTCCACCAAGACTTTCCTTTGATGACTTGGAATTTTTCATTGATATATGGACTGAAGATCGATTTCTCTTTTCTGAAGTGGTCCCTGGCCCAGTTCTACAGAAAGGTACCAAAATCCCACCACCAGGAGTATGTGACATGCTTAGATATCACCTGGAGGGTACCGAGTACAGGATGAAACTCCCTGTTGAGCCAAGGTTTGCTGTTCCTCTGACCCAGACCGTGAGTGTTTCAGTGCTTGTGGAGCGCAAAGATTCTAACAGGGTTGCACGTATAATTGATAATTCTATTTTTGACTACATTGATCGAACAGCATCTAGAGCCATGGCAAATGAATACCTAGACTTCTCGGCAGTCTACCCATTCACTTCAGGTATCCGGGCATGGATCTCTTTGCTTTTCATGGACAACGGAGATGAAGGAGTTATTGATGTTTTTGGGATCGAAATGGATTTCATGGATGCTGCGAACTCGAAGGAAGAGGTTTTGTGGCTATTAGACATGCTTGATTGGAAGTGA

Protein Analysis

250

Amino Acids

29.08

Weight (kDa)

6.34

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 7 - 44 1.2e-10 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 207
Acc36I ACCTGC 1 cut(s) 158
Acc65I GGTACC 2 cut(s) 338, 392
AccB1I GGYRCC 2 cut(s) 338, 392
AccB7I CCANNNNNTGG 1 cut(s) 138
AccI GTMKAC 1 cut(s) 588
AciI CCGC 2 cut(s) 26, 82
AclWI GGATC 2 cut(s) 626, 680
AcsI RAATTY 1 cut(s) 263
AcuI CTGAAG 4 cut(s) 41, 306, 327, 585
AfaI GTAC 3 cut(s) 340, 394, 401
AfiI CCNNNNNNNGG 5 cut(s) 25, 40, 138, 356, 402
AjnI CCWGG 3 cut(s) 316, 355, 384
AloI GAACNNNNNNTCC 2 cut(s) 217, 249
AluBI AGCT 2 cut(s) 98, 190
AluI AGCT 2 cut(s) 98, 190
Alw26I GTCTC 1 cut(s) 82
AlwI GGATC 2 cut(s) 626, 680
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 2 cut(s) 140, 319
ApeKI GCWGC 1 cut(s) 695
ApoI RAATTY 1 cut(s) 263
Asp718I GGTACC 2 cut(s) 338, 392
AspLEI GCGC 2 cut(s) 213, 480
AspS9I GGNCC 5 cut(s) 16, 52, 141, 313, 320
AsuC2I CCSGG 1 cut(s) 611
AsuHPI GGTGA 2 cut(s) 16, 374
AvaI CYCGRG 1 cut(s) 99
AvaII GGWCC 3 cut(s) 16, 52, 313
BanI GGYRCC 2 cut(s) 338, 392
BbvI GCAGC 1 cut(s) 682
BccI CCATC 1 cut(s) 132
BciT130I CCWGG 3 cut(s) 318, 357, 386
BciVI GTATCC 1 cut(s) 617
BcnI CCSGG 1 cut(s) 611
BcoDI GTCTC 1 cut(s) 82
BfaI CTAG 2 cut(s) 549, 572
BfmI CTRYAG 1 cut(s) 329
BfoI RGCGCY 1 cut(s) 214
BfuAI ACCTGC 1 cut(s) 158
BfuI GTATCC 1 cut(s) 617
BisI GCNGC 1 cut(s) 696
BlsI GCNGC 1 cut(s) 697
Bme1390I CCNGG 4 cut(s) 318, 357, 386, 611
Bme18I GGWCC 3 cut(s) 16, 52, 313
BmeT110I CYCGRG 1 cut(s) 99
BmgT120I GGNCC 5 cut(s) 16, 52, 141, 313, 320
BmiI GGNNCC 3 cut(s) 315, 340, 394
BmrFI CCNGG 4 cut(s) 318, 357, 386, 611
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 2 cut(s) 554, 682
BmuI ACTGGG 1 cut(s) 317
BpmI CTGGAG 2 cut(s) 62, 407
BpuMI CCSGG 1 cut(s) 611
Bsa29I ATCGAT 1 cut(s) 292
BsaAI YACGTR 1 cut(s) 503
BsaJI CCNNGG 3 cut(s) 316, 425, 555
Bsc4I CCNNNNNNNGG 5 cut(s) 25, 40, 138, 356, 402
Bse1I ACTGG 2 cut(s) 45, 323
BseBI CCWGG 3 cut(s) 318, 357, 386
BseCI ATCGAT 1 cut(s) 292
BseDI CCNNGG 3 cut(s) 316, 425, 555
BseGI GGATG 2 cut(s) 411, 697
BseLI CCNNNNNNNGG 5 cut(s) 25, 40, 138, 356, 402
BseNI ACTGG 2 cut(s) 45, 323
BseXI GCAGC 1 cut(s) 682
BshFI GGCC 2 cut(s) 142, 321
BshNI GGYRCC 2 cut(s) 338, 392
BshVI ATCGAT 1 cut(s) 292
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 1 cut(s) 610
BslFI GGGAC 1 cut(s) 299
BslI CCNNNNNNNGG 5 cut(s) 25, 40, 138, 356, 402
BsmAI GTCTC 1 cut(s) 82
BsmFI GGGAC 1 cut(s) 299
BsnI GGCC 2 cut(s) 142, 321
BsoBI CYCGRG 1 cut(s) 99
Bsp143I GATC 4 cut(s) 289, 535, 618, 672
Bsp19I CCATGG 1 cut(s) 555
BspACI CCGC 2 cut(s) 26, 82
BspANI GGCC 2 cut(s) 142, 321
BspDI ATCGAT 1 cut(s) 292
BspLI GGNNCC 3 cut(s) 315, 340, 394
BspMI ACCTGC 1 cut(s) 158
BspPI GGATC 2 cut(s) 626, 680
BspT107I GGYRCC 2 cut(s) 338, 392
BsrI ACTGG 2 cut(s) 45, 323
BssECI CCNNGG 3 cut(s) 316, 425, 555
BssMI GATC 4 cut(s) 289, 535, 618, 672
BssT1I CCWWGG 2 cut(s) 425, 555
Bst2UI CCWGG 3 cut(s) 318, 357, 386
Bst4CI ACNGT 2 cut(s) 221, 454
Bst6I CTCTTC 2 cut(s) 234, 705
BstBAI YACGTR 1 cut(s) 503
BstC8I GCNNGC 3 cut(s) 80, 155, 188
BstDEI CTNAG 1 cut(s) 374
BstDSI CCRYGG 1 cut(s) 555
BstF5I GGATG 2 cut(s) 411, 697
BstH2I RGCGCY 1 cut(s) 214
BstHHI GCGC 2 cut(s) 213, 480
BstKTI GATC 4 cut(s) 292, 538, 621, 675
BstMAI GTCTC 1 cut(s) 82
BstMBI GATC 4 cut(s) 289, 535, 618, 672
BstMWI GCNNNNNNNGC 2 cut(s) 475, 551
BstNI CCWGG 3 cut(s) 318, 357, 386
BstNSI RCATGY 2 cut(s) 373, 736
BstSCI CCNGG 4 cut(s) 316, 355, 384, 609
BstSFI CTRYAG 1 cut(s) 329
BstV1I GCAGC 1 cut(s) 682
BstX2I RGATCY 1 cut(s) 618
BstYI RGATCY 1 cut(s) 618
Bsu15I ATCGAT 1 cut(s) 292
BsuI GTATCC 1 cut(s) 617
BsuRI GGCC 2 cut(s) 142, 321
BsuTUI ATCGAT 1 cut(s) 292
BtgI CCRYGG 1 cut(s) 555
BtsCI GGATG 2 cut(s) 411, 697
BtsIMutI CAGTG 1 cut(s) 471
BveI ACCTGC 1 cut(s) 158
Cac8I GCNNGC 3 cut(s) 80, 155, 188
CfoI GCGC 2 cut(s) 213, 480
Cfr13I GGNCC 5 cut(s) 16, 52, 141, 313, 320
ClaI ATCGAT 1 cut(s) 292
CseI GACGC 1 cut(s) 110
Csp6I GTAC 3 cut(s) 339, 393, 400
CviAII CATG 6 cut(s) 370, 556, 615, 634, 688, 733
CviJI RGCY 7 cut(s) 98, 142, 190, 321, 424, 554, 724
CviKI_1 RGCY 7 cut(s) 98, 142, 190, 321, 424, 554, 724
CviQI GTAC 3 cut(s) 339, 393, 400
DdeI CTNAG 1 cut(s) 374
DpnI GATC 4 cut(s) 291, 537, 620, 674
DpnII GATC 4 cut(s) 289, 535, 618, 672
Eam1104I CTCTTC 2 cut(s) 234, 705
EarI CTCTTC 2 cut(s) 234, 705
Eco130I CCWWGG 2 cut(s) 425, 555
Eco32I GATATC 1 cut(s) 380
Eco47I GGWCC 3 cut(s) 16, 52, 313
Eco57I CTGAAG 4 cut(s) 41, 306, 327, 585
Eco88I CYCGRG 1 cut(s) 99
EcoRII CCWGG 3 cut(s) 316, 355, 384
EcoRV GATATC 1 cut(s) 380
EcoT14I CCWWGG 2 cut(s) 425, 555
ErhI CCWWGG 2 cut(s) 425, 555
FaeI CATG 6 cut(s) 373, 559, 618, 637, 691, 736
FaqI GGGAC 1 cut(s) 299
FatI CATG 6 cut(s) 369, 555, 614, 633, 687, 732
FblI GTMKAC 1 cut(s) 588
Fnu4HI GCNGC 1 cut(s) 696
FokI GGATG 2 cut(s) 418, 704
Fsp4HI GCNGC 1 cut(s) 696
FspBI CTAG 2 cut(s) 549, 572
GlaI GCGC 2 cut(s) 212, 479
GluI GCNGC 1 cut(s) 696
GsuI CTGGAG 2 cut(s) 62, 407
HaeII RGCGCY 1 cut(s) 214
HaeIII GGCC 2 cut(s) 142, 321
HapII CCGG 1 cut(s) 610
HgaI GACGC 1 cut(s) 110
HhaI GCGC 2 cut(s) 213, 480
Hin1II CATG 6 cut(s) 373, 559, 618, 637, 691, 736
Hin6I GCGC 2 cut(s) 211, 478
HinP1I GCGC 2 cut(s) 211, 478
HinfI GANTC 1 cut(s) 485
HpaII CCGG 1 cut(s) 610
HphI GGTGA 2 cut(s) 16, 374
Hpy166II GTNNAC 2 cut(s) 52, 589
Hpy188I TCNGA 2 cut(s) 307, 444
Hpy188III TCNNGA 3 cut(s) 19, 101, 549
Hpy8I GTNNAC 2 cut(s) 52, 589
Hpy99I CGWCG 1 cut(s) 225
HpyAV CCTTC 3 cut(s) 16, 644, 700
HpyCH4III ACNGT 2 cut(s) 221, 454
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 4 cut(s) 78, 153, 186, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 475, 551
HpyF3I CTNAG 1 cut(s) 374
HpySE526I ACGT 1 cut(s) 502
Hsp92II CATG 6 cut(s) 373, 559, 618, 637, 691, 736
HspAI GCGC 2 cut(s) 211, 478
KpnI GGTACC 2 cut(s) 342, 396
Kzo9I GATC 4 cut(s) 289, 535, 618, 672
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 1 cut(s) 682
LweI GCATC 2 cut(s) 554, 682
MaeI CTAG 2 cut(s) 549, 572
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 1 cut(s) 365
MalI GATC 4 cut(s) 291, 537, 620, 674
MboI GATC 4 cut(s) 289, 535, 618, 672
MboII GAAGA 4 cut(s) 221, 224, 299, 722
MflI RGATCY 1 cut(s) 618
MluCI AATT 3 cut(s) 263, 507, 514
MnlI CCTC 6 cut(s) 37, 154, 177, 382, 450, 706
MspI CCGG 1 cut(s) 610
MspR9I CCNGG 4 cut(s) 318, 357, 386, 611
MvaI CCWGG 3 cut(s) 318, 357, 386
MwoI GCNNNNNNNGC 2 cut(s) 475, 551
NciI CCSGG 1 cut(s) 611
NcoI CCATGG 1 cut(s) 555
NdeII GATC 4 cut(s) 289, 535, 618, 672
NlaIII CATG 6 cut(s) 373, 559, 618, 637, 691, 736
NlaIV GGNNCC 3 cut(s) 315, 340, 394
NmeAIII GCCGAG 1 cut(s) 560
NmuCI GTSAC 1 cut(s) 365
NspI RCATGY 2 cut(s) 373, 736
PaeR7I CTCGAG 1 cut(s) 99
PfeI GAWTC 1 cut(s) 485
PflMI CCANNNNNTGG 1 cut(s) 138
PkrI GCNGC 1 cut(s) 697
Ppu21I YACGTR 1 cut(s) 503
PsiI TTATAA 1 cut(s) 207
Psp6I CCWGG 3 cut(s) 316, 355, 384
PspGI CCWGG 3 cut(s) 316, 355, 384
PspN4I GGNNCC 3 cut(s) 315, 340, 394
PspPI GGNCC 5 cut(s) 16, 52, 141, 313, 320
PsuI RGATCY 1 cut(s) 618
RsaI GTAC 3 cut(s) 340, 394, 401
RsaNI GTAC 3 cut(s) 339, 393, 400
SatI GCNGC 1 cut(s) 696
Sau3AI GATC 4 cut(s) 289, 535, 618, 672
Sau96I GGNCC 5 cut(s) 16, 52, 141, 313, 320
ScrFI CCNGG 4 cut(s) 318, 357, 386, 611
SfaNI GCATC 2 cut(s) 554, 682
SfcI CTRYAG 1 cut(s) 329
Sfr274I CTCGAG 1 cut(s) 99
SinI GGWCC 3 cut(s) 16, 52, 313
SlaI CTCGAG 1 cut(s) 99
SmlI CTYRAG 1 cut(s) 99
SmoI CTYRAG 1 cut(s) 99
Sse9I AATT 3 cut(s) 263, 507, 514
SsiI CCGC 2 cut(s) 26, 82
SspMI CTAG 2 cut(s) 549, 572
StyD4I CCNGG 4 cut(s) 316, 355, 384, 609
StyI CCWWGG 2 cut(s) 425, 555
TaaI ACNGT 2 cut(s) 221, 454
TaiI ACGT 1 cut(s) 505
TaqI TCGA 6 cut(s) 100, 223, 292, 538, 675, 704
TasI AATT 3 cut(s) 263, 507, 514
TatI WGTACW 1 cut(s) 399
TfiI GAWTC 1 cut(s) 485
TscAI CASTG 1 cut(s) 471
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 1 cut(s) 695
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 7 cut(s) 24, 259, 422, 579, 622, 663, 676
TspGWI ACGGA 1 cut(s) 657
TspRI CASTG 1 cut(s) 471
Van91I CCANNNNNTGG 1 cut(s) 138
VpaK11BI GGWCC 3 cut(s) 16, 52, 313
XapI RAATTY 1 cut(s) 263
XbaI TCTAGA 1 cut(s) 548
XceI RCATGY 2 cut(s) 373, 736
XhoI CTCGAG 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 588
XspI CTAG 2 cut(s) 549, 572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.