FvH4_7g02300

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016380
Physical Location & Seq
Reverse (-)
2636863 .. 2637417
555 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02300.t1

Sequence Viewer

Length: 555 bp
ATGACTAGTTTTGTTACTTACGCCTACTTCATCATTCTATCAACCTCCTTCACAACCATTCATGGACTATTCTCAGAGCAATCTCCTATATCCCTGTCTGCATTACAAATGGAGAAACATACCCGCCTCCACTTCTATTTCCATGACATCCAAAGCGGCCAAAATCCGACTGCCATAAGAATTGTTAGGCCACCAAATAAGCCGGTTGATAGTTTCGGAACTACTTTTATTACAGATGATGTACTGACTGAAAAGCCAGAGGCTAGCTCAAAGCTTGTAGGGAGAGCTCAGGGCATTTATGCTTTTGCTTCTCAACATGACTCAGTGTTGCTCATGGTCCTGAATTTTGCTTTCATAGAGGGTATGTACAATGGCAGTACCCTTAGCATTCTTGGGAGGAATCCTTTTAAGAATGCCATGCGAGAGATGCCTATCGTAGGAGGCAGTGGAGTTTTCCGGTATGCACGGGGCTATGCACTAGCAAGGACATTCTCCTTCAATCCAAAGACCGGAGACGCTGTTGTCCAGTACAATGTGTCTGTGTTGCACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

185

Amino Acids

20.51

Weight (kDa)

9.19

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 41 - 181 3.4e-55 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016380)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02300
malus_domestica MD02G1295700.v1.1 MD07G1030100.v1.1
pyrus_communis pycom02g24840
rosa_chinensis RchiOBHm_Chr1g0319651
rosa_laevigata RLG00000030468
rosa_multiflora Rmu_sc0002539.1_g000096
rosa_roxburghii Rroxscaffold_4G00328250
rosa_rugosa Rorug01G0029100
rosa_samantha Rh1AG040800 Rh1BG038000 Rh1CG041900 Rh1DG028000
rosa_wichuraiana Rw0G019980 Rw1G003390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 521
AciI CCGC 2 cut(s) 124, 156
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 1 cut(s) 343
AfaI GTAC 4 cut(s) 243, 368, 379, 530
AfiI CCNNNNNNNGG 2 cut(s) 437, 509
AgsI TTSAA 1 cut(s) 499
AhlI ACTAGT 1 cut(s) 5
AluBI AGCT 3 cut(s) 267, 274, 287
AluI AGCT 3 cut(s) 267, 274, 287
Alw21I GWGCWC 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 507
AoxI GGCC 2 cut(s) 157, 188
ApoI RAATTY 1 cut(s) 343
AspS9I GGNCC 1 cut(s) 337
AsuNHI GCTAGC 1 cut(s) 263
AvaII GGWCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 289
Bbv12I GWGCWC 1 cut(s) 289
BcoDI GTCTC 1 cut(s) 507
BcuI ACTAGT 1 cut(s) 5
BfaI CTAG 4 cut(s) 6, 264, 479, 553
BisI GCNGC 1 cut(s) 157
BlsI GCNGC 1 cut(s) 158
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 337
BmsI GCATC 1 cut(s) 417
BmtI GCTAGC 1 cut(s) 267
BplI GAGNNNNNCTC 2 cut(s) 251, 283
Bpu10I CCTNAGC 2 cut(s) 288, 383
BsaBI GATNNNNATC 1 cut(s) 431
BsaWI WCCGGW 2 cut(s) 456, 509
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bsc4I CCNNNNNNNGG 2 cut(s) 437, 509
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 526
Bse8I GATNNNNATC 1 cut(s) 431
BseGI GGATG 1 cut(s) 147
BseJI GATNNNNATC 1 cut(s) 431
BseLI CCNNNNNNNGG 2 cut(s) 437, 509
BseMII CTCAG 3 cut(s) 87, 302, 336
BseNI ACTGG 1 cut(s) 526
BshFI GGCC 2 cut(s) 159, 190
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 3 cut(s) 203, 457, 510
BslI CCNNNNNNNGG 2 cut(s) 437, 509
BsmAI GTCTC 1 cut(s) 507
BsmBI CGTCTC 1 cut(s) 507
BsmI GAATGC 2 cut(s) 387, 418
BsnI GGCC 2 cut(s) 159, 190
Bsp1286I GDGCHC 1 cut(s) 289
Bsp1407I TGTACA 1 cut(s) 366
BspACI CCGC 2 cut(s) 124, 156
BspANI GGCC 2 cut(s) 159, 190
BspCNI CTCAG 3 cut(s) 86, 301, 335
BspOI GCTAGC 1 cut(s) 267
BsrFI RCCGGY 1 cut(s) 202
BsrGI TGTACA 1 cut(s) 366
BsrI ACTGG 1 cut(s) 526
BssAI RCCGGY 1 cut(s) 202
BstAUI TGTACA 1 cut(s) 366
BstC8I GCNNGC 1 cut(s) 265
BstDEI CTNAG 4 cut(s) 73, 288, 322, 383
BstENI CCTNNNNNAGG 1 cut(s) 435
BstF5I GGATG 1 cut(s) 147
BstMAI GTCTC 1 cut(s) 507
BstMWI GCNNNNNNNGC 1 cut(s) 427
BsuRI GGCC 2 cut(s) 159, 190
BtsCI GGATG 1 cut(s) 147
BtsI GCAGTG 1 cut(s) 451
BtsIMutI CAGTG 2 cut(s) 330, 451
Cac8I GCNNGC 1 cut(s) 265
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 337
CseI GACGC 1 cut(s) 524
Csp6I GTAC 4 cut(s) 242, 367, 378, 529
CviAII CATG 5 cut(s) 62, 143, 317, 334, 418
CviJI RGCY 9 cut(s) 159, 190, 202, 256, 263, 267, 274, 287, 471
CviKI_1 RGCY 9 cut(s) 159, 190, 202, 256, 263, 267, 274, 287, 471
CviQI GTAC 4 cut(s) 242, 367, 378, 529
DdeI CTNAG 4 cut(s) 73, 288, 322, 383
DrdI GACNNNNNNGTC 1 cut(s) 521
DseDI GACNNNNNNGTC 1 cut(s) 521
EaeI YGGCCR 1 cut(s) 157
Ecl136II GAGCTC 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 337
Eco53kI GAGCTC 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 287
EcoNI CCTNNNNNAGG 1 cut(s) 435
EcoT38I GRGCYC 1 cut(s) 289
Esp3I CGTCTC 1 cut(s) 507
FaeI CATG 5 cut(s) 65, 146, 320, 337, 421
FatI CATG 5 cut(s) 61, 142, 316, 333, 417
FauI CCCGC 1 cut(s) 131
Fnu4HI GCNGC 1 cut(s) 157
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 1 cut(s) 157
FspBI CTAG 4 cut(s) 6, 264, 479, 553
GluI GCNGC 1 cut(s) 157
HaeIII GGCC 2 cut(s) 159, 190
HapII CCGG 3 cut(s) 203, 457, 510
HgaI GACGC 1 cut(s) 524
Hin1II CATG 5 cut(s) 65, 146, 320, 337, 421
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 2 cut(s) 320, 400
HpaII CCGG 3 cut(s) 203, 457, 510
Hpy188I TCNGA 3 cut(s) 76, 168, 218
Hpy188III TCNNGA 1 cut(s) 340
HpyAV CCTTC 2 cut(s) 58, 505
HpyCH4V TGCA 4 cut(s) 101, 464, 476, 547
HpyF10VI GCNNNNNNNGC 1 cut(s) 427
HpyF3I CTNAG 4 cut(s) 73, 288, 322, 383
Hsp92II CATG 5 cut(s) 65, 146, 320, 337, 421
LpnPI CCDG 8 cut(s) 107, 216, 270, 275, 353, 470, 523, 539
LweI GCATC 1 cut(s) 417
MaeI CTAG 4 cut(s) 6, 264, 479, 553
MaeIII GTNAC 1 cut(s) 13
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 2 cut(s) 180, 343
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 191
MnlI CCTC 6 cut(s) 55, 137, 253, 352, 390, 434
MseI TTAA 1 cut(s) 408
MspI CCGG 3 cut(s) 203, 457, 510
Mva1269I GAATGC 2 cut(s) 387, 418
MwoI GCNNNNNNNGC 1 cut(s) 427
NheI GCTAGC 1 cut(s) 263
NlaIII CATG 5 cut(s) 65, 146, 320, 337, 421
PctI GAATGC 2 cut(s) 387, 418
PfeI GAWTC 1 cut(s) 400
PkrI GCNGC 1 cut(s) 158
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
Psp124BI GAGCTC 1 cut(s) 289
PspPI GGNCC 1 cut(s) 337
RsaI GTAC 4 cut(s) 243, 368, 379, 530
RsaNI GTAC 4 cut(s) 242, 367, 378, 529
SacI GAGCTC 1 cut(s) 289
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 1 cut(s) 157
Sau96I GGNCC 1 cut(s) 337
SchI GAGTC 1 cut(s) 314
SduI GDGCHC 1 cut(s) 289
SetI ASST 4 cut(s) 47, 269, 276, 289
SfaNI GCATC 1 cut(s) 417
SinI GGWCC 1 cut(s) 337
SpeI ACTAGT 1 cut(s) 5
Sse9I AATT 2 cut(s) 180, 343
SsiI CCGC 2 cut(s) 124, 156
SspMI CTAG 4 cut(s) 6, 264, 479, 553
SstI GAGCTC 1 cut(s) 289
TasI AATT 2 cut(s) 180, 343
TatI WGTACW 3 cut(s) 241, 366, 528
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TscAI CASTG 2 cut(s) 330, 451
TspDTI ATGAA 3 cut(s) 19, 50, 343
TspRI CASTG 2 cut(s) 330, 451
VpaK11BI GGWCC 1 cut(s) 337
XagI CCTNNNNNAGG 1 cut(s) 435
XapI RAATTY 1 cut(s) 343
XspI CTAG 4 cut(s) 6, 264, 479, 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.