Rw1G003390

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016380
Physical Location & Seq
Reverse (-)
6280938 .. 6281491
554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G003390.1

Sequence Viewer

Length: 525 bp
ATGGCTTTGTTTGTCCCGTTTGCCTGCTTCATCATTCTATCAACCTCCTTCACTACAATTCATGGACTATTCTCAGAGCAATCTCCTCTATCCCTATCTGCAATAAGAATGGAGAAACTTACCCGCGGCCAAAATCCAACTGCCCTAAGAATTCTTGGGCCACCAAACAAGTCGGTTGGTAGCTTCGGAACTACTTTCATAACAGACGATCCACTGACCGAAAAGCCAGAGGCTAGCTCAAAGCTTGTAGGAAGAGCACAGGGCATTTATGCTTTTGCTTCTCAACATGATTCAGGGTTGCTCATGGTCCTGAATTTTGCTTTCATAGAGGGTATGTACAATGGCAGTACCCTTAGCATTCTTGGGAGGAATCCTTTTATGGATACCATGCGAGAGATGCCTATAGTCGGAGGCAGTGGAGCTTTCCGGTATGCACGGGGCTATGCACTAGCAAGGACATTCTCCTTCAATCCAAAGACTGGAGATGCTGTTGTCCAGTACAATGTGTCAGTGTTGCACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

174

Amino Acids

18.95

Weight (kDa)

9.16

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 38 - 171 1.2e-49 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016380)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02300
malus_domestica MD02G1295700.v1.1 MD07G1030100.v1.1
pyrus_communis pycom02g24840
rosa_chinensis RchiOBHm_Chr1g0319651
rosa_laevigata RLG00000030468
rosa_multiflora Rmu_sc0002539.1_g000096
rosa_roxburghii Rroxscaffold_4G00328250
rosa_rugosa Rorug01G0029100
rosa_samantha Rh1AG040800 Rh1BG038000 Rh1CG041900 Rh1DG028000
rosa_wichuraiana Rw0G019980 Rw1G003390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 479
AccII CGCG 1 cut(s) 126
AciI CCGC 2 cut(s) 124, 126
AclWI GGATC 1 cut(s) 203
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 2 cut(s) 150, 313
AfaI GTAC 3 cut(s) 338, 349, 500
AfiI CCNNNNNNNGG 2 cut(s) 407, 479
AgsI TTSAA 1 cut(s) 469
AluBI AGCT 4 cut(s) 183, 237, 244, 422
AluI AGCT 4 cut(s) 183, 237, 244, 422
Alw21I GWGCWC 1 cut(s) 259
AlwI GGATC 1 cut(s) 203
AoxI GGCC 2 cut(s) 127, 158
ApoI RAATTY 2 cut(s) 150, 313
AspS9I GGNCC 2 cut(s) 158, 307
AsuNHI GCTAGC 1 cut(s) 233
AvaII GGWCC 1 cut(s) 307
Bbv12I GWGCWC 1 cut(s) 259
BciVI GTATCC 1 cut(s) 376
BfaI CTAG 3 cut(s) 234, 449, 523
BfmI CTRYAG 1 cut(s) 402
BfuI GTATCC 1 cut(s) 376
BisI GCNGC 1 cut(s) 127
BlsI GCNGC 1 cut(s) 128
Bme18I GGWCC 1 cut(s) 307
BmgT120I GGNCC 2 cut(s) 158, 307
BmsI GCATC 2 cut(s) 387, 475
BmtI GCTAGC 1 cut(s) 237
BplI GAGNNNNNCTC 2 cut(s) 221, 253
BpmI CTGGAG 1 cut(s) 501
Bpu10I CCTNAGC 1 cut(s) 353
BsaJI CCNNGG 1 cut(s) 124
BsaWI WCCGGW 1 cut(s) 426
BsaXI ACNNNNNCTCC 2 cut(s) 474, 504
Bsc4I CCNNNNNNNGG 2 cut(s) 407, 479
Bse1I ACTGG 2 cut(s) 484, 496
BseDI CCNNGG 1 cut(s) 124
BseLI CCNNNNNNNGG 2 cut(s) 407, 479
BseMII CTCAG 1 cut(s) 87
BseNI ACTGG 2 cut(s) 484, 496
BseRI GAGGAG 1 cut(s) 75
Bsh1236I CGCG 1 cut(s) 126
BshFI GGCC 2 cut(s) 129, 160
BsiHKAI GWGCWC 1 cut(s) 259
BsiSI CCGG 1 cut(s) 427
BslI CCNNNNNNNGG 2 cut(s) 407, 479
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 2 cut(s) 129, 160
Bsp1286I GDGCHC 1 cut(s) 259
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 1 cut(s) 208
BspACI CCGC 2 cut(s) 124, 126
BspANI GGCC 2 cut(s) 129, 160
BspCNI CTCAG 1 cut(s) 86
BspFNI CGCG 1 cut(s) 126
BspOI GCTAGC 1 cut(s) 237
BspPI GGATC 1 cut(s) 203
BspQI GCTCTTC 1 cut(s) 247
BsrGI TGTACA 1 cut(s) 336
BsrI ACTGG 2 cut(s) 484, 496
BssECI CCNNGG 1 cut(s) 124
BssMI GATC 1 cut(s) 208
Bst6I CTCTTC 1 cut(s) 247
BstAUI TGTACA 1 cut(s) 336
BstC8I GCNNGC 2 cut(s) 25, 235
BstDEI CTNAG 3 cut(s) 73, 146, 353
BstDSI CCRYGG 1 cut(s) 124
BstFNI CGCG 1 cut(s) 126
BstKTI GATC 1 cut(s) 211
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 1 cut(s) 397
BstSFI CTRYAG 1 cut(s) 402
BstUI CGCG 1 cut(s) 126
BsuI GTATCC 1 cut(s) 376
BsuRI GGCC 2 cut(s) 129, 160
BtgI CCRYGG 1 cut(s) 124
BtsI GCAGTG 1 cut(s) 421
BtsIMutI CAGTG 3 cut(s) 212, 421, 516
Cac8I GCNNGC 2 cut(s) 25, 235
Cfr13I GGNCC 2 cut(s) 158, 307
Cfr42I CCGCGG 1 cut(s) 127
Csp6I GTAC 3 cut(s) 337, 348, 499
CviAII CATG 4 cut(s) 62, 287, 304, 388
CviQI GTAC 3 cut(s) 337, 348, 499
DdeI CTNAG 3 cut(s) 73, 146, 353
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 307
EcoRI GAATTC 1 cut(s) 150
FaeI CATG 4 cut(s) 65, 290, 307, 391
FatI CATG 4 cut(s) 61, 286, 303, 387
FauI CCCGC 1 cut(s) 131
Fnu4HI GCNGC 1 cut(s) 127
Fsp4HI GCNGC 1 cut(s) 127
FspBI CTAG 3 cut(s) 234, 449, 523
GluI GCNGC 1 cut(s) 127
GsuI CTGGAG 1 cut(s) 501
HaeIII GGCC 2 cut(s) 129, 160
HapII CCGG 1 cut(s) 427
Hin1II CATG 4 cut(s) 65, 290, 307, 391
HindIII AAGCTT 1 cut(s) 242
HinfI GANTC 2 cut(s) 290, 370
HpaII CCGG 1 cut(s) 427
Hpy188I TCNGA 3 cut(s) 76, 188, 410
Hpy188III TCNNGA 1 cut(s) 310
HpyAV CCTTC 2 cut(s) 58, 475
HpyCH4V TGCA 4 cut(s) 101, 434, 446, 517
HpyF10VI GCNNNNNNNGC 1 cut(s) 397
HpyF3I CTNAG 3 cut(s) 73, 146, 353
Hsp92II CATG 4 cut(s) 65, 290, 307, 391
KspI CCGCGG 1 cut(s) 127
Kzo9I GATC 1 cut(s) 208
LguI GCTCTTC 1 cut(s) 247
LmnI GCTCC 1 cut(s) 419
LpnPI CCDG 8 cut(s) 37, 240, 245, 279, 323, 440, 465, 509
LweI GCATC 2 cut(s) 387, 475
MaeI CTAG 3 cut(s) 234, 449, 523
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 1 cut(s) 264
MhlI GDGCHC 1 cut(s) 259
MluCI AATT 3 cut(s) 57, 150, 313
MmeI TCCRAC 2 cut(s) 161, 388
MnlI CCTC 6 cut(s) 55, 96, 223, 322, 360, 404
MspA1I CMGCKG 1 cut(s) 126
MspI CCGG 1 cut(s) 427
Mva1269I GAATGC 1 cut(s) 357
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 1 cut(s) 397
NdeII GATC 1 cut(s) 208
NheI GCTAGC 1 cut(s) 233
NlaIII CATG 4 cut(s) 65, 290, 307, 391
PciSI GCTCTTC 1 cut(s) 247
PctI GAATGC 1 cut(s) 357
PfeI GAWTC 2 cut(s) 290, 370
PflMI CCANNNNNTGG 1 cut(s) 479
PkrI GCNGC 1 cut(s) 128
PspPI GGNCC 2 cut(s) 158, 307
RsaI GTAC 3 cut(s) 338, 349, 500
RsaNI GTAC 3 cut(s) 337, 348, 499
SacII CCGCGG 1 cut(s) 127
SapI GCTCTTC 1 cut(s) 247
SatI GCNGC 1 cut(s) 127
Sau3AI GATC 1 cut(s) 208
Sau96I GGNCC 2 cut(s) 158, 307
SduI GDGCHC 1 cut(s) 259
SetI ASST 5 cut(s) 47, 185, 239, 246, 424
SfaNI GCATC 2 cut(s) 387, 475
SfcI CTRYAG 1 cut(s) 402
Sfr303I CCGCGG 1 cut(s) 127
SgrBI CCGCGG 1 cut(s) 127
SinI GGWCC 1 cut(s) 307
Sse9I AATT 3 cut(s) 57, 150, 313
SsiI CCGC 2 cut(s) 124, 126
SspMI CTAG 3 cut(s) 234, 449, 523
TaqII GACCGA 1 cut(s) 233
TasI AATT 3 cut(s) 57, 150, 313
TatI WGTACW 2 cut(s) 336, 498
TauI GCSGC 1 cut(s) 129
TfiI GAWTC 2 cut(s) 290, 370
TscAI CASTG 3 cut(s) 219, 421, 516
TspDTI ATGAA 4 cut(s) 19, 50, 187, 313
TspRI CASTG 3 cut(s) 219, 421, 516
Van91I CCANNNNNTGG 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 307
XapI RAATTY 2 cut(s) 150, 313
XspI CTAG 3 cut(s) 234, 449, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.