RchiOBHm_Chr1g0319651

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016380
Physical Location & Seq
Reverse (-)
7286503 .. 7287332
830 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54987

Sequence Viewer

Length: 555 bp
ATGGCTTTGTTTGTCCCGTTTGCCTGCTTCATCATTCTATCAACCTCCTTCACTACAATTCATGGACTATTCTCAGAGCAATCTCCTCTATCCCTATCTGCAATAAGAATGGAGAAACTTACCCGCCTCCACTTCTATTTCCATGACATCCAAGGCGGCCAAAATCCAACTGCCCTAAGAATTCTTGGGCCACCAAACAAGTCGGTTGGTAGCTTCGGATCTACTTTCATAACAGACGATCCACTGACCGAGAAGCCAGAGGCTAGCTCAAAGCTTGTAGGAAGAGCTCAGGGCATTTATGCTTTTGCTTCTCAACATGATTCAGGGTTGCTCATGGTCCTGAATTTTGCTTTCATAGAGGGTATGTACAATGGCAGTACCCTTAGCATTCTTGGGAGGAATCCTTTTATGGATACCATGCGAGAGATGCCTATAGTCGGAGGCAGTGGAGCTTTCCGGTATGCACGGGGCTATGCACTAGCAAGGACATTCTCCTTCAATCCAAAGACTGGAGATGCTGTTGTCCAGTACAATGTGTCAGTGTTGCACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

184

Amino Acids

20.19

Weight (kDa)

8.76

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 41 - 181 8.6e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016380)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02300
malus_domestica MD02G1295700.v1.1 MD07G1030100.v1.1
pyrus_communis pycom02g24840
rosa_chinensis RchiOBHm_Chr1g0319651
rosa_laevigata RLG00000030468
rosa_multiflora Rmu_sc0002539.1_g000096
rosa_roxburghii Rroxscaffold_4G00328250
rosa_rugosa Rorug01G0029100
rosa_samantha Rh1AG040800 Rh1BG038000 Rh1CG041900 Rh1DG028000
rosa_wichuraiana Rw0G019980 Rw1G003390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 509
AciI CCGC 2 cut(s) 124, 156
AclWI GGATC 2 cut(s) 226, 233
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 2 cut(s) 180, 343
AfaI GTAC 3 cut(s) 368, 379, 530
AfiI CCNNNNNNNGG 2 cut(s) 437, 509
AgsI TTSAA 1 cut(s) 499
AluBI AGCT 5 cut(s) 213, 267, 274, 287, 452
AluI AGCT 5 cut(s) 213, 267, 274, 287, 452
Alw21I GWGCWC 1 cut(s) 289
AlwI GGATC 2 cut(s) 226, 233
AoxI GGCC 2 cut(s) 157, 188
ApoI RAATTY 2 cut(s) 180, 343
AspS9I GGNCC 2 cut(s) 188, 337
AsuNHI GCTAGC 1 cut(s) 263
AvaII GGWCC 1 cut(s) 337
BanII GRGCYC 1 cut(s) 289
Bbv12I GWGCWC 1 cut(s) 289
BciVI GTATCC 1 cut(s) 406
BfaI CTAG 3 cut(s) 264, 479, 553
BfmI CTRYAG 1 cut(s) 432
BfuI GTATCC 1 cut(s) 406
BisI GCNGC 1 cut(s) 157
BlsI GCNGC 1 cut(s) 158
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 2 cut(s) 188, 337
BmsI GCATC 2 cut(s) 417, 505
BmtI GCTAGC 1 cut(s) 267
BplI GAGNNNNNCTC 2 cut(s) 251, 283
BpmI CTGGAG 1 cut(s) 531
Bpu10I CCTNAGC 2 cut(s) 288, 383
BsaJI CCNNGG 1 cut(s) 151
BsaWI WCCGGW 1 cut(s) 456
BsaXI ACNNNNNCTCC 2 cut(s) 504, 534
Bsc4I CCNNNNNNNGG 2 cut(s) 437, 509
Bse1I ACTGG 2 cut(s) 514, 526
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 1 cut(s) 147
BseLI CCNNNNNNNGG 2 cut(s) 437, 509
BseMII CTCAG 2 cut(s) 87, 302
BseNI ACTGG 2 cut(s) 514, 526
BseRI GAGGAG 1 cut(s) 75
BshFI GGCC 2 cut(s) 159, 190
BsiHKAI GWGCWC 1 cut(s) 289
BsiSI CCGG 1 cut(s) 457
BslI CCNNNNNNNGG 2 cut(s) 437, 509
BsmI GAATGC 1 cut(s) 387
BsnI GGCC 2 cut(s) 159, 190
Bsp1286I GDGCHC 1 cut(s) 289
Bsp1407I TGTACA 1 cut(s) 366
Bsp143I GATC 2 cut(s) 218, 238
BspACI CCGC 2 cut(s) 124, 156
BspANI GGCC 2 cut(s) 159, 190
BspCNI CTCAG 2 cut(s) 86, 301
BspOI GCTAGC 1 cut(s) 267
BspPI GGATC 2 cut(s) 226, 233
BspQI GCTCTTC 1 cut(s) 277
BsrGI TGTACA 1 cut(s) 366
BsrI ACTGG 2 cut(s) 514, 526
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 2 cut(s) 218, 238
BssT1I CCWWGG 1 cut(s) 151
Bst6I CTCTTC 1 cut(s) 277
BstAUI TGTACA 1 cut(s) 366
BstC8I GCNNGC 2 cut(s) 25, 265
BstDEI CTNAG 4 cut(s) 73, 176, 288, 383
BstF5I GGATG 1 cut(s) 147
BstKTI GATC 2 cut(s) 221, 241
BstMBI GATC 2 cut(s) 218, 238
BstMWI GCNNNNNNNGC 1 cut(s) 427
BstSFI CTRYAG 1 cut(s) 432
BstX2I RGATCY 1 cut(s) 218
BstYI RGATCY 1 cut(s) 218
BsuI GTATCC 1 cut(s) 406
BsuRI GGCC 2 cut(s) 159, 190
BtsCI GGATG 1 cut(s) 147
BtsI GCAGTG 1 cut(s) 451
BtsIMutI CAGTG 3 cut(s) 242, 451, 546
Cac8I GCNNGC 2 cut(s) 25, 265
Cfr13I GGNCC 2 cut(s) 188, 337
Csp6I GTAC 3 cut(s) 367, 378, 529
CviAII CATG 5 cut(s) 62, 143, 317, 334, 418
CviQI GTAC 3 cut(s) 367, 378, 529
DdeI CTNAG 4 cut(s) 73, 176, 288, 383
DpnI GATC 2 cut(s) 220, 240
DpnII GATC 2 cut(s) 218, 238
EaeI YGGCCR 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 277
EarI CTCTTC 1 cut(s) 277
Ecl136II GAGCTC 1 cut(s) 287
Eco130I CCWWGG 1 cut(s) 151
Eco24I GRGCYC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 337
Eco53kI GAGCTC 1 cut(s) 287
EcoICRI GAGCTC 1 cut(s) 287
EcoRI GAATTC 1 cut(s) 180
EcoT14I CCWWGG 1 cut(s) 151
EcoT38I GRGCYC 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 151
FaeI CATG 5 cut(s) 65, 146, 320, 337, 421
FatI CATG 5 cut(s) 61, 142, 316, 333, 417
FauI CCCGC 1 cut(s) 131
Fnu4HI GCNGC 1 cut(s) 157
FokI GGATG 1 cut(s) 134
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 1 cut(s) 157
FspBI CTAG 3 cut(s) 264, 479, 553
GluI GCNGC 1 cut(s) 157
GsuI CTGGAG 1 cut(s) 531
HaeIII GGCC 2 cut(s) 159, 190
HapII CCGG 1 cut(s) 457
Hin1II CATG 5 cut(s) 65, 146, 320, 337, 421
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 2 cut(s) 320, 400
HpaII CCGG 1 cut(s) 457
Hpy188I TCNGA 3 cut(s) 76, 218, 440
Hpy188III TCNNGA 1 cut(s) 340
HpyAV CCTTC 2 cut(s) 58, 505
HpyCH4V TGCA 4 cut(s) 101, 464, 476, 547
HpyF10VI GCNNNNNNNGC 1 cut(s) 427
HpyF3I CTNAG 4 cut(s) 73, 176, 288, 383
Hsp92II CATG 5 cut(s) 65, 146, 320, 337, 421
Kzo9I GATC 2 cut(s) 218, 238
LguI GCTCTTC 1 cut(s) 277
LmnI GCTCC 1 cut(s) 449
LpnPI CCDG 8 cut(s) 37, 270, 275, 309, 353, 470, 495, 539
LweI GCATC 2 cut(s) 417, 505
MaeI CTAG 3 cut(s) 264, 479, 553
MalI GATC 2 cut(s) 220, 240
MboI GATC 2 cut(s) 218, 238
MboII GAAGA 1 cut(s) 294
MflI RGATCY 1 cut(s) 218
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 3 cut(s) 57, 180, 343
MmeI TCCRAC 2 cut(s) 191, 418
MnlI CCTC 7 cut(s) 55, 96, 137, 253, 352, 390, 434
MspI CCGG 1 cut(s) 457
Mva1269I GAATGC 1 cut(s) 387
MwoI GCNNNNNNNGC 1 cut(s) 427
NdeII GATC 2 cut(s) 218, 238
NheI GCTAGC 1 cut(s) 263
NlaIII CATG 5 cut(s) 65, 146, 320, 337, 421
PciSI GCTCTTC 1 cut(s) 277
PctI GAATGC 1 cut(s) 387
PfeI GAWTC 2 cut(s) 320, 400
PflMI CCANNNNNTGG 1 cut(s) 509
PkrI GCNGC 1 cut(s) 158
Psp124BI GAGCTC 1 cut(s) 289
PspPI GGNCC 2 cut(s) 188, 337
PsuI RGATCY 1 cut(s) 218
RsaI GTAC 3 cut(s) 368, 379, 530
RsaNI GTAC 3 cut(s) 367, 378, 529
SacI GAGCTC 1 cut(s) 289
SapI GCTCTTC 1 cut(s) 277
SatI GCNGC 1 cut(s) 157
Sau3AI GATC 2 cut(s) 218, 238
Sau96I GGNCC 2 cut(s) 188, 337
SduI GDGCHC 1 cut(s) 289
SetI ASST 6 cut(s) 47, 215, 269, 276, 289, 454
SfaNI GCATC 2 cut(s) 417, 505
SfcI CTRYAG 1 cut(s) 432
SinI GGWCC 1 cut(s) 337
Sse9I AATT 3 cut(s) 57, 180, 343
SsiI CCGC 2 cut(s) 124, 156
SspMI CTAG 3 cut(s) 264, 479, 553
SstI GAGCTC 1 cut(s) 289
StyI CCWWGG 1 cut(s) 151
TaqII GACCGA 1 cut(s) 263
TasI AATT 3 cut(s) 57, 180, 343
TatI WGTACW 2 cut(s) 366, 528
TauI GCSGC 1 cut(s) 159
TfiI GAWTC 2 cut(s) 320, 400
TscAI CASTG 3 cut(s) 249, 451, 546
TspDTI ATGAA 4 cut(s) 19, 50, 217, 343
TspRI CASTG 3 cut(s) 249, 451, 546
Van91I CCANNNNNTGG 1 cut(s) 509
VpaK11BI GGWCC 1 cut(s) 337
XapI RAATTY 2 cut(s) 180, 343
XspI CTAG 3 cut(s) 264, 479, 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.