Rroxscaffold_4G00328250

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016380
Physical Location & Seq
Forward (+)
61150632 .. 61151078
447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328250.1

Sequence Viewer

Length: 447 bp
ATGGAGAAACTTACCCGCCTCCACTTCTATTTCCATGACATCCAAGGCGGCCAAAATCCAACTGCCCTAAGAATTCTTGGGCCACCAAACAAGTCGGTTGGTAGCTTCGGAACTACTTTCATAACAGACGATCCACTGACCGAAAAGCCAGAGGCTAGCTCAAAGCTTGTAGGAAGAGCTCAGGGCATTTATGCTTTTGCTTCTCAACATGATTCAGGGTTGCTCATGGTCCTGAATTTTGCTTTCATAGAGGGTATGTACAATGGCAGTACCCTTAGCATTCTTGGGAGGAATCCTTTTATGGATACCATGCGAGAGATGCCTATAGTCGGAGGCAGTGGAGCTTTCCGGTATGCACGGGGCTATGCACTAGCAAGGACATTCTCCTTCAATCCAAAGACTGGAGATGCTGTTGTCCAGTACAATGTGTCAGTGTTGCACTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

148

Amino Acids

16.28

Weight (kDa)

8.96

Isoelectric Point (pI)

32.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 5 - 145 2.9e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016380)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02300
malus_domestica MD02G1295700.v1.1 MD07G1030100.v1.1
pyrus_communis pycom02g24840
rosa_chinensis RchiOBHm_Chr1g0319651
rosa_laevigata RLG00000030468
rosa_multiflora Rmu_sc0002539.1_g000096
rosa_roxburghii Rroxscaffold_4G00328250
rosa_rugosa Rorug01G0029100
rosa_samantha Rh1AG040800 Rh1BG038000 Rh1CG041900 Rh1DG028000
rosa_wichuraiana Rw0G019980 Rw1G003390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 401
AciI CCGC 2 cut(s) 16, 48
AclWI GGATC 1 cut(s) 125
AcoI YGGCCR 1 cut(s) 49
AcsI RAATTY 2 cut(s) 72, 235
AfaI GTAC 3 cut(s) 260, 271, 422
AfiI CCNNNNNNNGG 2 cut(s) 329, 401
AgsI TTSAA 1 cut(s) 391
AluBI AGCT 5 cut(s) 105, 159, 166, 179, 344
AluI AGCT 5 cut(s) 105, 159, 166, 179, 344
Alw21I GWGCWC 1 cut(s) 181
AlwI GGATC 1 cut(s) 125
AoxI GGCC 2 cut(s) 49, 80
ApoI RAATTY 2 cut(s) 72, 235
AspS9I GGNCC 2 cut(s) 80, 229
AsuNHI GCTAGC 1 cut(s) 155
AvaII GGWCC 1 cut(s) 229
BanII GRGCYC 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 181
BciVI GTATCC 1 cut(s) 298
BfaI CTAG 3 cut(s) 156, 371, 445
BfmI CTRYAG 1 cut(s) 324
BfuI GTATCC 1 cut(s) 298
BisI GCNGC 1 cut(s) 49
BlsI GCNGC 1 cut(s) 50
Bme18I GGWCC 1 cut(s) 229
BmgT120I GGNCC 2 cut(s) 80, 229
BmsI GCATC 2 cut(s) 309, 397
BmtI GCTAGC 1 cut(s) 159
BplI GAGNNNNNCTC 2 cut(s) 143, 175
BpmI CTGGAG 1 cut(s) 423
Bpu10I CCTNAGC 2 cut(s) 180, 275
BsaJI CCNNGG 1 cut(s) 43
BsaWI WCCGGW 1 cut(s) 348
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 401
Bse1I ACTGG 2 cut(s) 406, 418
BseDI CCNNGG 1 cut(s) 43
BseGI GGATG 1 cut(s) 39
BseLI CCNNNNNNNGG 2 cut(s) 329, 401
BseMII CTCAG 1 cut(s) 194
BseNI ACTGG 2 cut(s) 406, 418
BshFI GGCC 2 cut(s) 51, 82
BsiHKAI GWGCWC 1 cut(s) 181
BsiSI CCGG 1 cut(s) 349
BslI CCNNNNNNNGG 2 cut(s) 329, 401
BsmI GAATGC 1 cut(s) 279
BsnI GGCC 2 cut(s) 51, 82
Bsp1286I GDGCHC 1 cut(s) 181
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 1 cut(s) 130
BspACI CCGC 2 cut(s) 16, 48
BspANI GGCC 2 cut(s) 51, 82
BspCNI CTCAG 1 cut(s) 193
BspOI GCTAGC 1 cut(s) 159
BspPI GGATC 1 cut(s) 125
BspQI GCTCTTC 1 cut(s) 169
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 2 cut(s) 406, 418
BssECI CCNNGG 1 cut(s) 43
BssMI GATC 1 cut(s) 130
BssT1I CCWWGG 1 cut(s) 43
Bst6I CTCTTC 1 cut(s) 169
BstAUI TGTACA 1 cut(s) 258
BstC8I GCNNGC 1 cut(s) 157
BstDEI CTNAG 3 cut(s) 68, 180, 275
BstF5I GGATG 1 cut(s) 39
BstKTI GATC 1 cut(s) 133
BstMBI GATC 1 cut(s) 130
BstMWI GCNNNNNNNGC 1 cut(s) 319
BstSFI CTRYAG 1 cut(s) 324
BsuI GTATCC 1 cut(s) 298
BsuRI GGCC 2 cut(s) 51, 82
BtsCI GGATG 1 cut(s) 39
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 3 cut(s) 134, 343, 438
Cac8I GCNNGC 1 cut(s) 157
Cfr13I GGNCC 2 cut(s) 80, 229
Csp6I GTAC 3 cut(s) 259, 270, 421
CviAII CATG 4 cut(s) 35, 209, 226, 310
CviQI GTAC 3 cut(s) 259, 270, 421
DdeI CTNAG 3 cut(s) 68, 180, 275
DpnI GATC 1 cut(s) 132
DpnII GATC 1 cut(s) 130
EaeI YGGCCR 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 169
EarI CTCTTC 1 cut(s) 169
Ecl136II GAGCTC 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 43
Eco24I GRGCYC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 179
EcoICRI GAGCTC 1 cut(s) 179
EcoRI GAATTC 1 cut(s) 72
EcoT14I CCWWGG 1 cut(s) 43
EcoT38I GRGCYC 1 cut(s) 181
ErhI CCWWGG 1 cut(s) 43
FaeI CATG 4 cut(s) 38, 212, 229, 313
FatI CATG 4 cut(s) 34, 208, 225, 309
FauI CCCGC 1 cut(s) 23
Fnu4HI GCNGC 1 cut(s) 49
FokI GGATG 1 cut(s) 26
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 1 cut(s) 49
FspBI CTAG 3 cut(s) 156, 371, 445
GluI GCNGC 1 cut(s) 49
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 2 cut(s) 51, 82
HapII CCGG 1 cut(s) 349
Hin1II CATG 4 cut(s) 38, 212, 229, 313
HindIII AAGCTT 1 cut(s) 164
HinfI GANTC 2 cut(s) 212, 292
HpaII CCGG 1 cut(s) 349
Hpy188I TCNGA 2 cut(s) 110, 332
Hpy188III TCNNGA 1 cut(s) 232
HpyAV CCTTC 1 cut(s) 397
HpyCH4V TGCA 3 cut(s) 356, 368, 439
HpyF10VI GCNNNNNNNGC 1 cut(s) 319
HpyF3I CTNAG 3 cut(s) 68, 180, 275
Hsp92II CATG 4 cut(s) 38, 212, 229, 313
Kzo9I GATC 1 cut(s) 130
LguI GCTCTTC 1 cut(s) 169
LmnI GCTCC 1 cut(s) 341
LpnPI CCDG 7 cut(s) 162, 167, 201, 245, 362, 387, 431
LweI GCATC 2 cut(s) 309, 397
MaeI CTAG 3 cut(s) 156, 371, 445
MalI GATC 1 cut(s) 132
MboI GATC 1 cut(s) 130
MboII GAAGA 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 181
MluCI AATT 2 cut(s) 72, 235
MmeI TCCRAC 2 cut(s) 83, 310
MnlI CCTC 5 cut(s) 29, 145, 244, 282, 326
MspI CCGG 1 cut(s) 349
Mva1269I GAATGC 1 cut(s) 279
MwoI GCNNNNNNNGC 1 cut(s) 319
NdeII GATC 1 cut(s) 130
NheI GCTAGC 1 cut(s) 155
NlaIII CATG 4 cut(s) 38, 212, 229, 313
PciSI GCTCTTC 1 cut(s) 169
PctI GAATGC 1 cut(s) 279
PfeI GAWTC 2 cut(s) 212, 292
PflMI CCANNNNNTGG 1 cut(s) 401
PkrI GCNGC 1 cut(s) 50
Psp124BI GAGCTC 1 cut(s) 181
PspPI GGNCC 2 cut(s) 80, 229
RsaI GTAC 3 cut(s) 260, 271, 422
RsaNI GTAC 3 cut(s) 259, 270, 421
SacI GAGCTC 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 169
SatI GCNGC 1 cut(s) 49
Sau3AI GATC 1 cut(s) 130
Sau96I GGNCC 2 cut(s) 80, 229
SduI GDGCHC 1 cut(s) 181
SetI ASST 5 cut(s) 107, 161, 168, 181, 346
SfaNI GCATC 2 cut(s) 309, 397
SfcI CTRYAG 1 cut(s) 324
SinI GGWCC 1 cut(s) 229
Sse9I AATT 2 cut(s) 72, 235
SsiI CCGC 2 cut(s) 16, 48
SspMI CTAG 3 cut(s) 156, 371, 445
SstI GAGCTC 1 cut(s) 181
StyI CCWWGG 1 cut(s) 43
TaqII GACCGA 1 cut(s) 155
TasI AATT 2 cut(s) 72, 235
TatI WGTACW 2 cut(s) 258, 420
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 2 cut(s) 212, 292
TscAI CASTG 3 cut(s) 141, 343, 438
TspDTI ATGAA 2 cut(s) 109, 235
TspRI CASTG 3 cut(s) 141, 343, 438
Van91I CCANNNNNTGG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 229
XapI RAATTY 2 cut(s) 72, 235
XspI CTAG 3 cut(s) 156, 371, 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.