MD02G1295700.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016380
Physical Location & Seq
Forward (+)
35008770 .. 35009327
558 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1295700.v1.1.491

Sequence Viewer

Length: 558 bp
ATGGTTTGTTTTGCCACTTTTGCCTGCTACTCCATCATCTTATCAACCTCCTTCAGTACCATTCATGGAATATTCTCAGAGCAATCTCTTTTATCCCTATCTGCAAAACGAATGGAGAAAACTACCAGCCTTCACTTCTATTTCCATGACATCCACGACGGCGAGAACCCAACTGCCATGAAAATTGTTGAACCACCAAACGATTCAGTTGGTGGTTTCGGAACAACTTTCATGGCAGACAATCCATTGACAGAAGGGCCAGAGCCTAGTTCAAAGCTTGTGGGGAGAGCTCAGGGGATTTATGCTGTTGCTTCTCAGCATGACTCAGGGTTGCTTATGGTCTTGAACTTTGCTTTTCTAGACGGTATGTACAATGGTAGTACCGTAAGCATTCTAGGGAGGAATCCATTTATGGAAGCCATCCGAGAGATGCCTATTGTTGGAGGTACTGGGGTTTTCCAATATGCACGGGGCTATGCATTGGCGAAGACATTTTCGATGAGCATGAAAACTGGAGATGCTGTTGTTGAGTACAATGTGTCTGTGTTGCACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

186

Amino Acids

20.12

Weight (kDa)

5.6

Isoelectric Point (pI)

40.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 42 - 182 3e-56 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016380)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02300
malus_domestica MD02G1295700.v1.1 MD07G1030100.v1.1
pyrus_communis pycom02g24840
rosa_chinensis RchiOBHm_Chr1g0319651
rosa_laevigata RLG00000030468
rosa_multiflora Rmu_sc0002539.1_g000096
rosa_roxburghii Rroxscaffold_4G00328250
rosa_rugosa Rorug01G0029100
rosa_samantha Rh1AG040800 Rh1BG038000 Rh1CG041900 Rh1DG028000
rosa_wichuraiana Rw0G019980 Rw1G003390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 37
AfaI GTAC 5 cut(s) 58, 371, 382, 448, 533
AfiI CCNNNNNNNGG 1 cut(s) 440
AgsI TTSAA 3 cut(s) 191, 273, 346
AluBI AGCT 2 cut(s) 277, 290
AluI AGCT 2 cut(s) 277, 290
Alw21I GWGCWC 1 cut(s) 292
AoxI GGCC 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 257
BaeI ACNNNNGTAYC 2 cut(s) 438, 471
BanII GRGCYC 1 cut(s) 292
BbsI GAAGAC 1 cut(s) 494
Bbv12I GWGCWC 1 cut(s) 292
BccI CCATC 2 cut(s) 41, 428
BceAI ACGGC 1 cut(s) 175
BfaI CTAG 3 cut(s) 267, 359, 395
BmgT120I GGNCC 1 cut(s) 257
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 2 cut(s) 420, 508
BmuI ACTGGG 1 cut(s) 459
BpiI GAAGAC 1 cut(s) 494
BpmI CTGGAG 1 cut(s) 534
Bpu10I CCTNAGC 1 cut(s) 291
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 1 cut(s) 440
Bse1I ACTGG 2 cut(s) 454, 517
BseGI GGATG 2 cut(s) 150, 420
BseLI CCNNNNNNNGG 1 cut(s) 440
BseMII CTCAG 4 cut(s) 90, 305, 329, 339
BseNI ACTGG 2 cut(s) 454, 517
BshFI GGCC 1 cut(s) 259
BsiHKAI GWGCWC 1 cut(s) 292
BslI CCNNNNNNNGG 1 cut(s) 440
BsmI GAATGC 1 cut(s) 390
BsnI GGCC 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 292
Bsp1407I TGTACA 1 cut(s) 369
BspANI GGCC 1 cut(s) 259
BspCNI CTCAG 4 cut(s) 89, 304, 328, 338
BsrGI TGTACA 1 cut(s) 369
BsrI ACTGG 2 cut(s) 454, 517
Bst4CI ACNGT 2 cut(s) 365, 385
BstAUI TGTACA 1 cut(s) 369
BstC8I GCNNGC 1 cut(s) 25
BstDEI CTNAG 4 cut(s) 76, 291, 315, 325
BstF5I GGATG 2 cut(s) 150, 420
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstV2I GAAGAC 1 cut(s) 494
BsuRI GGCC 1 cut(s) 259
BtsCI GGATG 2 cut(s) 150, 420
Cac8I GCNNGC 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 257
Csp6I GTAC 5 cut(s) 57, 370, 381, 447, 532
CspCI CAANNNNNGTGG 2 cut(s) 261, 296
CviAII CATG 6 cut(s) 65, 146, 178, 232, 320, 505
CviJI RGCY 7 cut(s) 129, 259, 265, 277, 290, 419, 474
CviKI_1 RGCY 7 cut(s) 129, 259, 265, 277, 290, 419, 474
CviQI GTAC 5 cut(s) 57, 370, 381, 447, 532
DdeI CTNAG 4 cut(s) 76, 291, 315, 325
Ecl136II GAGCTC 1 cut(s) 290
Eco24I GRGCYC 1 cut(s) 292
Eco53kI GAGCTC 1 cut(s) 290
Eco57I CTGAAG 1 cut(s) 37
EcoICRI GAGCTC 1 cut(s) 290
EcoT22I ATGCAT 1 cut(s) 481
EcoT38I GRGCYC 1 cut(s) 292
FaeI CATG 6 cut(s) 68, 149, 181, 235, 323, 508
FatI CATG 6 cut(s) 64, 145, 177, 231, 319, 504
FokI GGATG 2 cut(s) 137, 407
FriOI GRGCYC 1 cut(s) 292
FspBI CTAG 3 cut(s) 267, 359, 395
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 1 cut(s) 259
Hin1II CATG 6 cut(s) 68, 149, 181, 235, 323, 508
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 3 cut(s) 203, 323, 403
Hpy188I TCNGA 3 cut(s) 79, 221, 425
Hpy188III TCNNGA 2 cut(s) 343, 359
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 3 cut(s) 61, 140, 248
HpyCH4III ACNGT 2 cut(s) 365, 385
HpyCH4V TGCA 4 cut(s) 104, 467, 479, 550
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 4 cut(s) 76, 291, 315, 325
Hsp92II CATG 6 cut(s) 68, 149, 181, 235, 323, 508
LpnPI CCDG 7 cut(s) 37, 139, 273, 278, 312, 435, 498
LweI GCATC 2 cut(s) 420, 508
MaeI CTAG 3 cut(s) 267, 359, 395
MboII GAAGA 1 cut(s) 499
MhlI GDGCHC 1 cut(s) 292
MluCI AATT 1 cut(s) 183
MlyI GAGTC 1 cut(s) 317
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 3 cut(s) 58, 393, 437
Mph1103I ATGCAT 1 cut(s) 481
Mva1269I GAATGC 1 cut(s) 390
MwoI GCNNNNNNNGC 1 cut(s) 20
NlaIII CATG 6 cut(s) 68, 149, 181, 235, 323, 508
NsiI ATGCAT 1 cut(s) 481
PctI GAATGC 1 cut(s) 390
PfeI GAWTC 2 cut(s) 203, 403
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
Psp124BI GAGCTC 1 cut(s) 292
PspPI GGNCC 1 cut(s) 257
RsaI GTAC 5 cut(s) 58, 371, 382, 448, 533
RsaNI GTAC 5 cut(s) 57, 370, 381, 447, 532
SacI GAGCTC 1 cut(s) 292
Sau96I GGNCC 1 cut(s) 257
SchI GAGTC 1 cut(s) 317
SduI GDGCHC 1 cut(s) 292
SetI ASST 4 cut(s) 50, 279, 292, 448
SfaNI GCATC 2 cut(s) 420, 508
Sse9I AATT 1 cut(s) 183
SspI AATATT 1 cut(s) 72
SspMI CTAG 3 cut(s) 267, 359, 395
SstI GAGCTC 1 cut(s) 292
TaaI ACNGT 2 cut(s) 365, 385
TaqI TCGA 1 cut(s) 497
TasI AATT 1 cut(s) 183
TatI WGTACW 2 cut(s) 369, 531
TfiI GAWTC 2 cut(s) 203, 403
TspDTI ATGAA 4 cut(s) 53, 194, 220, 521
XbaI TCTAGA 1 cut(s) 358
XspI CTAG 3 cut(s) 267, 359, 395
Zsp2I ATGCAT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.