FvH4_7g07350

At5g48480

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014792
Physical Location & Seq
Forward (+)
7297831 .. 7301961
4131 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g07350.t1

Sequence Viewer

Length: 867 bp
ATGGCAGAAGAATTGAAAATTTTGGAGGCCATACCACAGCTCATGGTGGCTTCAGATAAAGCCGTTGAAGCTGCTGATTTTTATGGACTCTTATTCAGAGCAGAGATTATCCGACACAATGAGTTCCCAGTGCAGAATCGAACTTCTGATAGCTACTACAAGGTTGCATGTGTGGAGCTCAGAATAGGCTCCAGCTCCCTTGTGGTGAGAGATATGGATACTATTAACAGCCGAGTTGTCAGAAATGTTGCATATGTTTCCACTTGCCTCCGCGTTCAATGCTTGAACGTTGACCTTTTGATGGAAAAAGCTGTCAATATGGGCTGCACAGTTGTTAAGGAGATATTGGAGGATCCTGTTTGTCCTGGTGTGTTGGTGAGCATCATAAGGGATCCGTTCGGTCACATATGGGAACTTAGTGACTCATCATGTGGTGTCTCAGAAGATGGTTTCATGCATAATGAGTGCATGAATGTGTTGCTCCAAGGTGGGAGCAACACTGAGAGAAAGTATAACCAAGAGCTGCTTGAATCCTATCAATGCACACCAGTAGTTGGGTCCTGCAAGACAATTAAAAATGCCACAGATGTTATCCCCACAGTCAGAATTGGAGGTTCATTTGACCCCATTCTATTTGACTGCAAATATATGGCAGTCTTAACATGGCTACAAGAGGAGCTGAGTTACGAAGGAACACTTGTTGGGCTTGCTGATCCATCTACTTCATTCAAAGACAGGGAGAGATGTCTCGATAAAGGAGCCGATTTAGTTGTGGAAAAGCCCCTGAATTTGAAAAAGATTACTCATATTCTGTTTCAGTCTCTGAAGAAGAAGAAGAAGATAAAGGAGAAGATGAAGTTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3-dmu-9_3-mt Glyoxalase

Protein Analysis

289

Amino Acids

32.33

Weight (kDa)

5.72

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g48480-like_N PF22656 13 - 72 1.5e-06 Glyoxalase At5g48480-like, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 554
AccII CGCG 1 cut(s) 273
AciI CCGC 1 cut(s) 271
AclI AACGTT 1 cut(s) 288
AclWI GGATC 5 cut(s) 347, 360, 386, 399, 707
AcsI RAATTY 2 cut(s) 18, 787
AcuI CTGAAG 2 cut(s) 36, 845
AfiI CCNNNNNNNGG 2 cut(s) 301, 554
AgsI TTSAA 7 cut(s) 16, 68, 278, 286, 530, 730, 793
AjnI CCWGG 1 cut(s) 364
AluBI AGCT 8 cut(s) 40, 71, 153, 178, 195, 311, 523, 679
AluI AGCT 8 cut(s) 40, 71, 153, 178, 195, 311, 523, 679
Alw21I GWGCWC 1 cut(s) 180
Alw26I GTCTC 3 cut(s) 442, 752, 825
AlwI GGATC 5 cut(s) 347, 360, 386, 399, 707
AlwNI CAGNNNCTG 1 cut(s) 823
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 3 cut(s) 71, 324, 523
ApoI RAATTY 2 cut(s) 18, 787
AspS9I GGNCC 1 cut(s) 558
AsuHPI GGTGA 2 cut(s) 217, 388
AvaII GGWCC 1 cut(s) 558
BamHI GGATCC 2 cut(s) 352, 391
BanII GRGCYC 1 cut(s) 180
Bbv12I GWGCWC 1 cut(s) 180
BbvI GCAGC 3 cut(s) 58, 311, 510
BccI CCATC 3 cut(s) 295, 440, 724
BceAI ACGGC 1 cut(s) 47
BciT130I CCWGG 1 cut(s) 366
BciVI GTATCC 1 cut(s) 211
BcoDI GTCTC 3 cut(s) 442, 752, 825
BfuI GTATCC 1 cut(s) 211
BisI GCNGC 3 cut(s) 72, 325, 524
BlsI GCNGC 3 cut(s) 73, 326, 525
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 1 cut(s) 558
BmgT120I GGNCC 1 cut(s) 558
BmiI GGNNCC 5 cut(s) 190, 354, 393, 559, 760
BmrFI CCNGG 1 cut(s) 366
BmrI ACTGGG 1 cut(s) 122
BmsI GCATC 1 cut(s) 390
BmuI ACTGGG 1 cut(s) 122
BpmI CTGGAG 1 cut(s) 175
BsaJI CCNNGG 1 cut(s) 484
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 554
Bse1I ACTGG 2 cut(s) 128, 548
BseBI CCWGG 1 cut(s) 366
BseDI CCNNGG 1 cut(s) 484
BseLI CCNNNNNNNGG 2 cut(s) 301, 554
BseMII CTCAG 4 cut(s) 193, 453, 492, 671
BseNI ACTGG 2 cut(s) 128, 548
BseRI GAGGAG 1 cut(s) 689
BseXI GCAGC 3 cut(s) 58, 311, 510
BsgI GTGCAG 2 cut(s) 152, 310
Bsh1236I CGCG 1 cut(s) 273
BshFI GGCC 1 cut(s) 29
BsiHKAI GWGCWC 1 cut(s) 180
BslI CCNNNNNNNGG 2 cut(s) 301, 554
BsmAI GTCTC 3 cut(s) 442, 752, 825
BsnI GGCC 1 cut(s) 29
Bsp1286I GDGCHC 1 cut(s) 180
Bsp143I GATC 3 cut(s) 352, 391, 712
BspACI CCGC 1 cut(s) 271
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 4 cut(s) 192, 452, 493, 672
BspFNI CGCG 1 cut(s) 273
BspLI GGNNCC 5 cut(s) 190, 354, 393, 559, 760
BspPI GGATC 5 cut(s) 347, 360, 386, 399, 707
BsrI ACTGG 2 cut(s) 128, 548
BssECI CCNNGG 1 cut(s) 484
BssMI GATC 3 cut(s) 352, 391, 712
BssT1I CCWWGG 1 cut(s) 484
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 3 cut(s) 331, 601, 864
BstC8I GCNNGC 1 cut(s) 708
BstDEI CTNAG 5 cut(s) 179, 416, 439, 501, 680
BstFNI CGCG 1 cut(s) 273
BstKTI GATC 3 cut(s) 355, 394, 715
BstMAI GTCTC 3 cut(s) 442, 752, 825
BstMBI GATC 3 cut(s) 352, 391, 712
BstMWI GCNNNNNNNGC 2 cut(s) 68, 279
BstNI CCWGG 1 cut(s) 366
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 1 cut(s) 364
BstUI CGCG 1 cut(s) 273
BstV1I GCAGC 3 cut(s) 58, 311, 510
BstX2I RGATCY 2 cut(s) 352, 391
BstYI RGATCY 2 cut(s) 352, 391
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 1 cut(s) 29
BtsIMutI CAGTG 2 cut(s) 135, 498
Cac8I GCNNGC 1 cut(s) 708
CaiI CAGNNNCTG 1 cut(s) 823
Cfr13I GGNCC 1 cut(s) 558
CviAII CATG 6 cut(s) 43, 168, 429, 454, 469, 663
DdeI CTNAG 5 cut(s) 179, 416, 439, 501, 680
DpnI GATC 3 cut(s) 354, 393, 714
DpnII GATC 3 cut(s) 352, 391, 712
Ecl136II GAGCTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 484
Eco24I GRGCYC 1 cut(s) 180
Eco47I GGWCC 1 cut(s) 558
Eco53kI GAGCTC 1 cut(s) 178
Eco57I CTGAAG 2 cut(s) 36, 845
EcoICRI GAGCTC 1 cut(s) 178
EcoO109I RGGNCCY 1 cut(s) 558
EcoRII CCWGG 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 484
EcoT22I ATGCAT 1 cut(s) 459
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 1 cut(s) 484
FaeI CATG 6 cut(s) 46, 171, 432, 457, 472, 666
FalI AAGNNNNNCTT 4 cut(s) 510, 542, 681, 713
FatI CATG 6 cut(s) 42, 167, 428, 453, 468, 662
FauNDI CATATG 2 cut(s) 253, 407
Fnu4HI GCNGC 3 cut(s) 72, 325, 524
FriOI GRGCYC 1 cut(s) 180
Fsp4HI GCNGC 3 cut(s) 72, 325, 524
GluI GCNGC 3 cut(s) 72, 325, 524
GsuI CTGGAG 1 cut(s) 175
HaeIII GGCC 1 cut(s) 29
Hin1II CATG 6 cut(s) 46, 171, 432, 457, 472, 666
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 4 cut(s) 87, 136, 422, 530
HphI GGTGA 2 cut(s) 217, 388
Hpy166II GTNNAC 1 cut(s) 292
Hpy188I TCNGA 9 cut(s) 55, 98, 113, 148, 182, 242, 442, 605, 825
Hpy188III TCNNGA 1 cut(s) 749
Hpy8I GTNNAC 1 cut(s) 292
HpyAV CCTTC 1 cut(s) 683
HpyCH4III ACNGT 3 cut(s) 331, 601, 864
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 9 cut(s) 133, 167, 251, 327, 457, 468, 543, 564, 642
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 279
HpyF3I CTNAG 5 cut(s) 179, 416, 439, 501, 680
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 6 cut(s) 46, 171, 432, 457, 472, 666
Kzo9I GATC 3 cut(s) 352, 391, 712
LmnI GCTCC 7 cut(s) 175, 194, 200, 486, 492, 676, 758
LpnPI CCDG 9 cut(s) 141, 205, 351, 369, 378, 561, 574, 721, 797
Lsp1109I GCAGC 3 cut(s) 58, 311, 510
LweI GCATC 1 cut(s) 390
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 4 cut(s) 401, 419, 683, 858
MalI GATC 3 cut(s) 354, 393, 714
MboI GATC 3 cut(s) 352, 391, 712
MboII GAAGA 8 cut(s) 20, 455, 838, 841, 844, 847, 850, 862
MflI RGATCY 2 cut(s) 352, 391
MhlI GDGCHC 1 cut(s) 180
MluCI AATT 5 cut(s) 11, 18, 570, 606, 787
MlyI GAGTC 2 cut(s) 81, 416
MmeI TCCRAC 1 cut(s) 136
MnlI CCTC 5 cut(s) 19, 278, 343, 605, 667
Mph1103I ATGCAT 1 cut(s) 459
MseI TTAA 4 cut(s) 225, 336, 573, 659
MslI CAYNNNNRTG 1 cut(s) 473
MspR9I CCNGG 1 cut(s) 366
MvaI CCWGG 1 cut(s) 366
MvnI CGCG 1 cut(s) 273
MwoI GCNNNNNNNGC 2 cut(s) 68, 279
NdeI CATATG 2 cut(s) 253, 407
NdeII GATC 3 cut(s) 352, 391, 712
NlaIII CATG 6 cut(s) 46, 171, 432, 457, 472, 666
NlaIV GGNNCC 5 cut(s) 190, 354, 393, 559, 760
NmeAIII GCCGAG 1 cut(s) 257
NmuCI GTSAC 2 cut(s) 401, 419
NsiI ATGCAT 1 cut(s) 459
NspI RCATGY 1 cut(s) 171
PfeI GAWTC 2 cut(s) 136, 530
PflMI CCANNNNNTGG 1 cut(s) 554
PkrI GCNGC 3 cut(s) 73, 326, 525
PleI GAGTC 2 cut(s) 81, 416
PpsI GAGTC 2 cut(s) 81, 416
PpuMI RGGWCCY 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 180
Psp1406I AACGTT 1 cut(s) 288
Psp5II RGGWCCY 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 364
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 5 cut(s) 190, 354, 393, 559, 760
PspPI GGNCC 1 cut(s) 558
PspPPI RGGWCCY 1 cut(s) 558
PstNI CAGNNNCTG 1 cut(s) 823
PsuI RGATCY 2 cut(s) 352, 391
RseI CAYNNNNRTG 1 cut(s) 473
SacI GAGCTC 1 cut(s) 180
SaqAI TTAA 4 cut(s) 225, 336, 573, 659
SatI GCNGC 3 cut(s) 72, 325, 524
Sau3AI GATC 3 cut(s) 352, 391, 712
Sau96I GGNCC 1 cut(s) 558
SchI GAGTC 2 cut(s) 81, 416
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 1 cut(s) 180
SfaNI GCATC 1 cut(s) 390
SinI GGWCC 1 cut(s) 558
SmiMI CAYNNNNRTG 1 cut(s) 473
Sse9I AATT 5 cut(s) 11, 18, 570, 606, 787
SsiI CCGC 1 cut(s) 271
SstI GAGCTC 1 cut(s) 180
StyD4I CCNGG 1 cut(s) 364
StyI CCWWGG 1 cut(s) 484
TaaI ACNGT 3 cut(s) 331, 601, 864
TaiI ACGT 1 cut(s) 291
TaqI TCGA 2 cut(s) 139, 750
TaqII GACCGA 1 cut(s) 389
TasI AATT 5 cut(s) 11, 18, 570, 606, 787
TfiI GAWTC 2 cut(s) 136, 530
Tru1I TTAA 4 cut(s) 225, 336, 573, 659
Tru9I TTAA 4 cut(s) 225, 336, 573, 659
TscAI CASTG 2 cut(s) 135, 505
TseFI GTSAC 2 cut(s) 401, 419
TseI GCWGC 3 cut(s) 71, 324, 523
Tsp45I GTSAC 2 cut(s) 401, 419
TspDTI ATGAA 4 cut(s) 442, 485, 606, 714
TspGWI ACGGA 1 cut(s) 384
TspRI CASTG 2 cut(s) 135, 505
Van91I CCANNNNNTGG 1 cut(s) 554
VpaK11BI GGWCC 1 cut(s) 558
XapI RAATTY 2 cut(s) 18, 787
XceI RCATGY 1 cut(s) 171
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.