FvH4_7g11810

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005583
Physical Location & Seq
Forward (+)
10809806 .. 10816711
6906 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g11810.t4

Sequence Viewer

Length: 1935 bp
ATGCTTGGATCAACCAGAACTGAGAATTCCAAGTCTTGGAAGGTACTCATCATGGATAAAGTAACGGTGAAAGTCATGTCTCATTCATGTAAAATGGCAGATATCACTGACCAAGAAATTTCCTTGGTGGAAGACCTTTTTCGGAGGAGGGAACCATTGCCGTCAATGGATGTTATATACTATATACAGCCATCAAAAGAGAATGTTGTCATGTTCTTGTCTGACATGTCTGGAAGGGAGCCTTTATACAGGAAAGCCTTTGTATTTTTCAGTTCACCTATCCCAAAGGAACTCATAAATCACATAAAGAAAGATACAAGTGTTTTACCGCGTATAGGTGCATTGAGAGAGATGAATCTAGAATACTTTCCCATAGATAATCAGGCTTTTGTCACTGATCAAGATAAGGCATTGGAGGAGCTCTTTGGTAATGTTGACAATTCTAAGAGAATCGAGAAGTGCTTGGACATAATGGCAACTCGAATTGCGACAGTTTTTGCTTCAATGAAGTTTACATTACCTCAGGAGCTGCCATCGGTACGATACCATGCTTCCAAGGCAGTGGATGAATCCGAAGCATCACCTCGTGATTTATTTCCTACAAAGCTTGCTGGTGCTGTTTGGAACATTATTTCAAAATATAAATCTTCAATTCCCAACTTTCCACAGAAAGAAACATGCGACTTACTCATCTTGGATAGATCTGTTGATCAGATTGCTCCTGTCATACATGAGTGGACGTATGATGCTATGTGCCACGATTTATTAGAAATGGATGGCAACAAATATATACATGAGGTTCCTAGCAAAACTGGTGGAGATCCTGAGAGAAAGGAGGTCCTTTTAGAAGACAATGATCCTGTCTGGTTGGAGATTCGCCATACACATATAGCAGATGCTAGTGAACGATTGAGCGACAAGTTTAGCACCTTTCAATCAAAGAATAAAGCTGCACAGCTCCAACAGAGTGCAAGAGATGGCAATGAATTATCTACACGGGATTTGCAGAAAATGGTTCAAGCTCTGCCACAGTACACTGAACAAATGGAAAAGATTTCTCTCCATGTCGAGATTGCAGGGAAAATTAACAGAATTATCCAGGAAAAGGGACTTCGAGACCTTGGGCAACTAGAGCAGGATCTTGTTTTTGGAGATGCCGGAGCCAAGGATGTCATCAATTATTTGAGGACCAATCAGGATGCAAGTTCTGAAAATAAGTTGCGTTTGTTAATGATTTATGCCTCTATATATCCTGAGAAGTTTGAAGGGGACAAAGCTAGTAAGCTAATGCAGTTGGCAAAGTTATCACAAGAGGACATGAAGGTTGTGAATAACATGAAAATGCTTAAGGGATCATCAGAATCCAAAAAGACGACTGGTAGCTTTTCACTCAAGTTCAATAATGCGAAGACTAAACAAGCAGCGAGGAAGGATCGTGAAGGTGAAGAGGAGGAAACATGGCAACTATTTCGATTTTTTCCACTTATGGAGGAGCTCATTGAAAATCTTAACAAAGGTCTATTACCAAAGAATGAGTATTCATGTATGAATGGACCTGGTCCAACTCCTGACTCAGTAAGCCAGAGGCGCACTCAGAGTACATCTGAAACTGCAACTCCATCAACTAATCCCCATTCGATAAGATCGAGGAGAACTGCAAACTGGGGACGAGCTCGGCATTCGGATGATGGATATTCAAGTGACTCGATTTTGAGAGGTGAGTCTACTGATTACAAGAAGTTGGGGCGACGTATTTTTGTATTTATGATTGGTGGGGCGACTCGATCAGAGCTACGAGTTTGTTACAAGATGACAACAAAATTGAAAAGGGAAGTAATCCTTGGAACGACCAGTATCCAAGAACCACCACAGTATCTTACGAAACTGAAGCTGCTATCAGAAAAAGAACTGTCAGTTGATGGCCTCAAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 map04721
Pfam Domains
Protein Families
Sec1

Protein Analysis

645

Amino Acids

73.4

Weight (kDa)

7.6

Isoelectric Point (pI)

51.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 46 - 654 1.3e-129 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 36
AccI GTMKAC 1 cut(s) 1724
AccII CGCG 1 cut(s) 331
AciI CCGC 1 cut(s) 329
AclWI GGATC 6 cut(s) 16, 815, 851, 1146, 1360, 1440
AcsI RAATTY 2 cut(s) 25, 117
AcuI CTGAAG 1 cut(s) 1907
AdeI CACNNNGTG 1 cut(s) 587
AfaI GTAC 4 cut(s) 45, 540, 1034, 1600
AfiI CCNNNNNNNGG 3 cut(s) 36, 335, 1105
AflII CTTAAG 1 cut(s) 1346
AflIII ACRYGT 1 cut(s) 225
AjnI CCWGG 2 cut(s) 1098, 1555
AjuI GAANNNNNNNTTGG 2 cut(s) 1824, 1856
Alw21I GWGCWC 3 cut(s) 423, 1497, 1675
Alw26I GTCTC 2 cut(s) 84, 1110
AlwI GGATC 6 cut(s) 16, 815, 851, 1146, 1360, 1440
AlwNI CAGNNNCTG 1 cut(s) 529
AoxI GGCC 1 cut(s) 1921
ApeKI GCWGC 4 cut(s) 529, 950, 1421, 1891
ApoI RAATTY 2 cut(s) 25, 117
Asp700I GAANNNNTTC 1 cut(s) 366
AspLEI GCGC 1 cut(s) 1590
AspS9I GGNCC 4 cut(s) 838, 1188, 1553, 1559
AsuHPI GGTGA 5 cut(s) 79, 267, 573, 1454, 1730
AvaII GGWCC 4 cut(s) 838, 1188, 1553, 1559
AxyI CCTNAGG 1 cut(s) 522
BaeI ACNNNNGTAYC 4 cut(s) 306, 339, 1856, 1889
BanII GRGCYC 3 cut(s) 423, 1497, 1675
BauI CACGAG 1 cut(s) 585
BbsI GAAGAC 3 cut(s) 138, 855, 1415
Bbv12I GWGCWC 3 cut(s) 423, 1497, 1675
BbvI GCAGC 4 cut(s) 516, 937, 1433, 1878
BccI CCATC 7 cut(s) 199, 541, 770, 971, 1627, 1682, 1913
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 2 cut(s) 1451, 1485
BciT130I CCWGG 2 cut(s) 1100, 1557
BciVI GTATCC 1 cut(s) 1865
BclI TGATCA 2 cut(s) 397, 709
BcoDI GTCTC 2 cut(s) 84, 1110
BfaI CTAG 5 cut(s) 359, 804, 900, 1130, 1278
BfrI CTTAAG 1 cut(s) 1346
BfuI GTATCC 1 cut(s) 1865
BglII AGATCT 1 cut(s) 701
BisI GCNGC 4 cut(s) 530, 951, 1422, 1892
BlsI GCNGC 4 cut(s) 531, 952, 1423, 1893
Bme1390I CCNGG 2 cut(s) 1100, 1557
Bme18I GGWCC 4 cut(s) 838, 1188, 1553, 1559
BmgT120I GGNCC 4 cut(s) 838, 1188, 1553, 1559
BmiI GGNNCC 4 cut(s) 153, 240, 801, 1162
BmrFI CCNGG 2 cut(s) 1100, 1557
BmrI ACTGGG 1 cut(s) 1672
BmsI GCATC 5 cut(s) 587, 736, 886, 1144, 1189
BmuI ACTGGG 1 cut(s) 1672
BpiI GAAGAC 3 cut(s) 138, 855, 1415
BplI GAGNNNNNCTC 2 cut(s) 1576, 1608
BpuEI CTTGAG 1 cut(s) 1376
BsaI GGTCTC 1 cut(s) 1110
BsaJI CCNNGG 5 cut(s) 123, 555, 1120, 1164, 1840
BsaXI ACNNNNNCTCC 2 cut(s) 1600, 1630
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 335, 1105
Bse1I ACTGG 4 cut(s) 817, 1381, 1667, 1851
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 2 cut(s) 155, 988
BseBI CCWGG 2 cut(s) 1100, 1557
BseDI CCNNGG 5 cut(s) 123, 555, 1120, 1164, 1840
BseGI GGATG 6 cut(s) 175, 571, 781, 1174, 1204, 1690
BseLI CCNNNNNNNGG 3 cut(s) 36, 335, 1105
BseMI GCAATG 2 cut(s) 155, 988
BseMII CTCAG 6 cut(s) 12, 536, 816, 1245, 1587, 1607
BseNI ACTGG 4 cut(s) 817, 1381, 1667, 1851
BseRI GAGGAG 5 cut(s) 160, 431, 1463, 1505, 1663
BseXI GCAGC 4 cut(s) 516, 937, 1433, 1878
BsgI GTGCAG 1 cut(s) 936
Bsh1236I CGCG 1 cut(s) 331
BshFI GGCC 1 cut(s) 1923
BsiHKAI GWGCWC 3 cut(s) 423, 1497, 1675
BsiSI CCGG 1 cut(s) 1158
BslFI GGGAC 3 cut(s) 1122, 1283, 1680
BslI CCNNNNNNNGG 3 cut(s) 36, 335, 1105
BsmAI GTCTC 2 cut(s) 84, 1110
BsmFI GGGAC 3 cut(s) 1122, 1283, 1680
BsmI GAATGC 1 cut(s) 1678
BsnI GGCC 1 cut(s) 1923
Bso31I GGTCTC 1 cut(s) 1110
Bsp1286I GDGCHC 3 cut(s) 423, 1497, 1675
BspACI CCGC 1 cut(s) 329
BspANI GGCC 1 cut(s) 1923
BspCNI CTCAG 6 cut(s) 13, 535, 817, 1246, 1586, 1606
BspFNI CGCG 1 cut(s) 331
BspLI GGNNCC 4 cut(s) 153, 240, 801, 1162
BspPI GGATC 6 cut(s) 16, 815, 851, 1146, 1360, 1440
BspTI CTTAAG 1 cut(s) 1346
BspTNI GGTCTC 1 cut(s) 1110
BsrDI GCAATG 2 cut(s) 155, 988
BsrI ACTGG 4 cut(s) 817, 1381, 1667, 1851
BssECI CCNNGG 5 cut(s) 123, 555, 1120, 1164, 1840
BssSI CACGAG 1 cut(s) 585
BssT1I CCWWGG 5 cut(s) 123, 555, 1120, 1164, 1840
Bst2BI CACGAG 1 cut(s) 585
Bst2UI CCWGG 2 cut(s) 1100, 1557
Bst4CI ACNGT 5 cut(s) 67, 493, 1032, 1872, 1911
Bst6I CTCTTC 1 cut(s) 1440
BstAFI CTTAAG 1 cut(s) 1346
BstC8I GCNNGC 1 cut(s) 609
BstDEI CTNAG 7 cut(s) 21, 444, 522, 825, 1254, 1573, 1593
BstF5I GGATG 6 cut(s) 175, 571, 781, 1174, 1204, 1690
BstFNI CGCG 1 cut(s) 331
BstHHI GCGC 1 cut(s) 1590
BstMAI GTCTC 2 cut(s) 84, 1110
BstMWI GCNNNNNNNGC 3 cut(s) 557, 1132, 1587
BstNI CCWGG 2 cut(s) 1100, 1557
BstNSI RCATGY 2 cut(s) 229, 681
BstSCI CCNGG 2 cut(s) 1098, 1555
BstUI CGCG 1 cut(s) 331
BstV1I GCAGC 4 cut(s) 516, 937, 1433, 1878
BstV2I GAAGAC 3 cut(s) 138, 855, 1415
BstX2I RGATCY 3 cut(s) 701, 820, 1138
BstXI CCANNNNNNTGG 1 cut(s) 562
BstYI RGATCY 3 cut(s) 701, 820, 1138
Bsu36I CCTNAGG 1 cut(s) 522
BsuI GTATCC 1 cut(s) 1865
BsuRI GGCC 1 cut(s) 1923
BtsCI GGATG 6 cut(s) 175, 571, 781, 1174, 1204, 1690
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 4 cut(s) 105, 393, 567, 1035
Cac8I GCNNGC 1 cut(s) 609
CaiI CAGNNNCTG 1 cut(s) 529
CfoI GCGC 1 cut(s) 1590
Cfr13I GGNCC 4 cut(s) 838, 1188, 1553, 1559
CsiI ACCWGGT 1 cut(s) 1555
Csp6I GTAC 4 cut(s) 44, 539, 1033, 1599
CspCI CAANNNNNGTGG 2 cut(s) 796, 831
CviQI GTAC 4 cut(s) 44, 539, 1033, 1599
DdeI CTNAG 7 cut(s) 21, 444, 522, 825, 1254, 1573, 1593
DraIII CACNNNGTG 1 cut(s) 587
Eam1104I CTCTTC 1 cut(s) 1440
EarI CTCTTC 1 cut(s) 1440
Ecl136II GAGCTC 3 cut(s) 421, 1495, 1673
Eco130I CCWWGG 5 cut(s) 123, 555, 1120, 1164, 1840
Eco24I GRGCYC 3 cut(s) 423, 1497, 1675
Eco31I GGTCTC 1 cut(s) 1110
Eco32I GATATC 1 cut(s) 103
Eco47I GGWCC 4 cut(s) 838, 1188, 1553, 1559
Eco53kI GAGCTC 3 cut(s) 421, 1495, 1673
Eco57I CTGAAG 1 cut(s) 1907
Eco81I CCTNAGG 1 cut(s) 522
EcoICRI GAGCTC 3 cut(s) 421, 1495, 1673
EcoO109I RGGNCCY 1 cut(s) 838
EcoRI GAATTC 1 cut(s) 25
EcoRII CCWGG 2 cut(s) 1098, 1555
EcoRV GATATC 1 cut(s) 103
EcoT14I CCWWGG 5 cut(s) 123, 555, 1120, 1164, 1840
EcoT38I GRGCYC 3 cut(s) 423, 1497, 1675
ErhI CCWWGG 5 cut(s) 123, 555, 1120, 1164, 1840
FalI AAGNNNNNCTT 4 cut(s) 226, 258, 1824, 1856
FaqI GGGAC 3 cut(s) 1122, 1283, 1680
FbaI TGATCA 2 cut(s) 397, 709
FblI GTMKAC 1 cut(s) 1724
Fnu4HI GCNGC 4 cut(s) 530, 951, 1422, 1892
FokI GGATG 6 cut(s) 182, 578, 788, 1181, 1211, 1697
FriOI GRGCYC 3 cut(s) 423, 1497, 1675
Fsp4HI GCNGC 4 cut(s) 530, 951, 1422, 1892
FspBI CTAG 5 cut(s) 359, 804, 900, 1130, 1278
GlaI GCGC 1 cut(s) 1589
GluI GCNGC 4 cut(s) 530, 951, 1422, 1892
HaeIII GGCC 1 cut(s) 1923
HapII CCGG 1 cut(s) 1158
HhaI GCGC 1 cut(s) 1590
Hin6I GCGC 1 cut(s) 1588
HinP1I GCGC 1 cut(s) 1588
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HindIII AAGCTT 1 cut(s) 605
HinfI GANTC 9 cut(s) 355, 450, 569, 874, 1361, 1571, 1703, 1721, 1780
HpaII CCGG 1 cut(s) 1158
HphI GGTGA 5 cut(s) 79, 267, 573, 1454, 1730
Hpy166II GTNNAC 7 cut(s) 275, 436, 513, 738, 905, 1035, 1725
Hpy8I GTNNAC 7 cut(s) 275, 436, 513, 738, 905, 1035, 1725
Hpy99I CGWCG 1 cut(s) 1752
HpyAV CCTTC 6 cut(s) 34, 228, 1259, 1315, 1423, 1433
HpyCH4III ACNGT 5 cut(s) 67, 493, 1032, 1872, 1911
HpyCH4IV ACGT 2 cut(s) 740, 1750
HpyCH4V TGCA 9 cut(s) 341, 953, 971, 1006, 1076, 1202, 1291, 1613, 1658
HpyF10VI GCNNNNNNNGC 3 cut(s) 557, 1132, 1587
HpyF3I CTNAG 7 cut(s) 21, 444, 522, 825, 1254, 1573, 1593
HpySE526I ACGT 2 cut(s) 740, 1750
HspAI GCGC 1 cut(s) 1588
Ksp22I TGATCA 2 cut(s) 397, 709
LmnI GCTCC 7 cut(s) 238, 418, 526, 724, 963, 1160, 1492
Lsp1109I GCAGC 4 cut(s) 516, 937, 1433, 1878
LweI GCATC 5 cut(s) 587, 736, 886, 1144, 1189
MabI ACCWGGT 1 cut(s) 1555
MaeI CTAG 5 cut(s) 359, 804, 900, 1130, 1278
MaeII ACGT 2 cut(s) 740, 1750
MaeIII GTNAC 4 cut(s) 61, 391, 1700, 1802
MboII GAAGA 5 cut(s) 143, 639, 860, 1420, 1457
MflI RGATCY 3 cut(s) 701, 820, 1138
MhlI GDGCHC 3 cut(s) 423, 1497, 1675
MlyI GAGTC 4 cut(s) 1565, 1697, 1730, 1774
MmeI TCCRAC 3 cut(s) 849, 985, 1586
MroXI GAANNNNTTC 1 cut(s) 366
MseI TTAA 4 cut(s) 1086, 1229, 1347, 1509
MslI CAYNNNNRTG 2 cut(s) 1340, 1683
MspCI CTTAAG 1 cut(s) 1346
MspI CCGG 1 cut(s) 1158
MspR9I CCNGG 2 cut(s) 1100, 1557
Mva1269I GAATGC 1 cut(s) 1678
MvaI CCWGG 2 cut(s) 1100, 1557
MvnI CGCG 1 cut(s) 331
MwoI GCNNNNNNNGC 3 cut(s) 557, 1132, 1587
NlaIV GGNNCC 4 cut(s) 153, 240, 801, 1162
NmeAIII GCCGAG 1 cut(s) 1654
NmuCI GTSAC 2 cut(s) 391, 1700
NspI RCATGY 2 cut(s) 229, 681
PciI ACATGT 1 cut(s) 225
PcsI WCGNNNNNNNCGW 1 cut(s) 1643
PctI GAATGC 1 cut(s) 1678
PdmI GAANNNNTTC 1 cut(s) 366
PfeI GAWTC 5 cut(s) 355, 450, 569, 874, 1361
PflFI GACNNNGTC 1 cut(s) 1557
PflMI CCANNNNNTGG 1 cut(s) 36
PfoI TCCNGGA 1 cut(s) 1098
PkrI GCNGC 4 cut(s) 531, 952, 1423, 1893
PleI GAGTC 4 cut(s) 1565, 1697, 1729, 1774
PpsI GAGTC 4 cut(s) 1565, 1697, 1729, 1774
PpuMI RGGWCCY 1 cut(s) 838
PscI ACATGT 1 cut(s) 225
Psp124BI GAGCTC 3 cut(s) 423, 1497, 1675
Psp5II RGGWCCY 1 cut(s) 838
Psp6I CCWGG 2 cut(s) 1098, 1555
PspGI CCWGG 2 cut(s) 1098, 1555
PspN4I GGNNCC 4 cut(s) 153, 240, 801, 1162
PspPI GGNCC 4 cut(s) 838, 1188, 1553, 1559
PspPPI RGGWCCY 1 cut(s) 838
PstNI CAGNNNCTG 1 cut(s) 529
PsuI RGATCY 3 cut(s) 701, 820, 1138
PsyI GACNNNGTC 1 cut(s) 1557
RsaI GTAC 4 cut(s) 45, 540, 1034, 1600
RsaNI GTAC 4 cut(s) 44, 539, 1033, 1599
RseI CAYNNNNRTG 2 cut(s) 1340, 1683
SacI GAGCTC 3 cut(s) 423, 1497, 1675
SaqAI TTAA 4 cut(s) 1086, 1229, 1347, 1509
SatI GCNGC 4 cut(s) 530, 951, 1422, 1892
Sau96I GGNCC 4 cut(s) 838, 1188, 1553, 1559
SchI GAGTC 4 cut(s) 1565, 1697, 1730, 1774
ScrFI CCNGG 2 cut(s) 1100, 1557
SduI GDGCHC 3 cut(s) 423, 1497, 1675
SexAI ACCWGGT 1 cut(s) 1555
SfaNI GCATC 5 cut(s) 587, 736, 886, 1144, 1189
SinI GGWCC 4 cut(s) 838, 1188, 1553, 1559
SmiMI CAYNNNNRTG 2 cut(s) 1340, 1683
SmlI CTYRAG 2 cut(s) 1346, 1391
SmoI CTYRAG 2 cut(s) 1346, 1391
SsiI CCGC 1 cut(s) 329
SspMI CTAG 5 cut(s) 359, 804, 900, 1130, 1278
SstI GAGCTC 3 cut(s) 423, 1497, 1675
StyD4I CCNGG 2 cut(s) 1098, 1555
StyI CCWWGG 5 cut(s) 123, 555, 1120, 1164, 1840
TaaI ACNGT 5 cut(s) 67, 493, 1032, 1872, 1911
TaiI ACGT 2 cut(s) 743, 1753
TaqI TCGA 9 cut(s) 453, 481, 1068, 1114, 1471, 1637, 1646, 1706, 1783
TatI WGTACW 2 cut(s) 1032, 1598
TfiI GAWTC 5 cut(s) 355, 450, 569, 874, 1361
Tru1I TTAA 4 cut(s) 1086, 1229, 1347, 1509
Tru9I TTAA 4 cut(s) 1086, 1229, 1347, 1509
TscAI CASTG 4 cut(s) 112, 400, 567, 1042
TseFI GTSAC 2 cut(s) 391, 1700
TseI GCWGC 4 cut(s) 529, 950, 1421, 1891
Tsp45I GTSAC 2 cut(s) 391, 1700
TspDTI ATGAA 9 cut(s) 75, 368, 521, 582, 999, 1334, 1352, 1530, 1562
TspRI CASTG 4 cut(s) 112, 400, 567, 1042
Tth111I GACNNNGTC 1 cut(s) 1557
Van91I CCANNNNNTGG 1 cut(s) 36
Vha464I CTTAAG 1 cut(s) 1346
VpaK11BI GGWCC 4 cut(s) 838, 1188, 1553, 1559
XapI RAATTY 2 cut(s) 25, 117
XbaI TCTAGA 1 cut(s) 358
XceI RCATGY 2 cut(s) 229, 681
XmiI GTMKAC 1 cut(s) 1724
XmnI GAANNNNTTC 1 cut(s) 366
XspI CTAG 5 cut(s) 359, 804, 900, 1130, 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.