Rroxscaffold_4G00304830

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005583
Physical Location & Seq
Reverse (-)
25452677 .. 25459542
6866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00304830.1

Sequence Viewer

Length: 1671 bp
ATGGCGGAGTCTCGGAGTACAAGTGTTTCCGTCAGTTCAGCCGTGACCGGTTATTGCACGAAATGCTTGGATCAACCAGAACGGAGAATTCCAAGTCTTGGAAGTGGTGGAAGACCTTTTCGAAGGAGGGAGCCATTGCCGTCAATGGATGTTATCTATTTTATCCAGCCAACAAAAGAGAATATTGTCATGTTCTTGTCTGACATGTCTGGAAGGGAGCCTTTATACAGGAAAGCGTTCATATTTTTCAGTTCACCTATCCCAAAGGAACTTGTAAATCACATAAAGAGTGATACAAGTGTTTTACCACGTATAGGTGCATTGAGAGAGATGAATTTGGAATACTTTCCCATAGATAATCAGGCTTTTATCACTGATCAAGATAGGGCATTGGAGGAGCTTTTTGGTGATGTTGAGAATTCTAAGACAGTCGAGAAGTGCTTGGACATAATGGCAACTCGAATTGCAACGGTTTTTGCTTCAATGAAGGAGCTGCCATATGTGCGATATCGTGCTTCCAAGGCACTGGATGAATCTGCAGAAGCAACACCTCGTGATTTAATTCCTACAAAGCTTGCTACTGCTGTTTGGAACATTCTTTCAAAATATAAATCTTCTATTCCCAACTTTCCACAGAAAGAAACATGCGACTTACTCATTTTGGATAGAACTGTTGATCAGATTGCTCCTGTCATACACGAATGGACATATGATGCTATGTGTCACGATTTATTGTATATGGATGGCAACAAATATGTGCATGAGGTTCCTAGCAAAACTGGTGGAGATCCTGAGAGAAAGGAAGTCTTTTTGGAAGATAATGATCCTGTATGGCTTGAGATTCGCCATACACATATAGCAGATGCTAGTGAACGATTGAGTGACAAGTTCTCCACCTTTCAATCAAAGAATAAAGCTGCACAGCTCCAACAGAGTGCAAGAGATGGCAATGAATTATCTACACGGGACTTGCAGAAAATGGTTCAAGCTCTGCCACAGTACACTGAACAAATGGAAAAGATTTCTCTCCATGTTGAGATTGCAGGAAAACTTAACAAAGTTATCAGGGAAACGGGACTTCGAAACCTCGGGCAACTGGAGCAGGACCTTGTTTTTGGAGATGCAGGAGCCAAGGATGTCATCAATTTTCTGAGGACCAATCAGGATACAAGTCCTGAAAATAAGTTGCGTTTGCTAATGATTTATGCCTCTGTATATCCTGAGAAGTTTGAAGGGGACAAAGCTAGTAAGCTAATGCAGTTGGCAAGGTTATCACATGAGGACATGAAGGTTGTGAATAACATGCGATCGCTTAAGGGATCATCAGATTCCAAAAAGACAACTGGTAGCTTTTCACTCAAGTTCAATAATGCGAAGACTAAACAAGCAGCAAGAAAGGATCGTGAAGGTGAAGAGGAGGAAACGTGGCAACTATTCCGATTTTTTCCACTGATGGAGGAACTCATTGAAAATCTTAACAAAGGTCTATTACCAAAGGATGAGTACTCATGTATGAATGAACCTGGTCCAACTCCTGAGCCAGGAAGCCAGAGGCGTACTCAGAGTACATCTGAAAGGTCAACTCAGTCGACTAATCCCCATTCGATAAGATCAAGGAGAACTGCAAATTGGGGACGGGCTCGGCATTCTGATGATGGATATTCAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 map04721
Pfam Domains
Protein Families
Sec1

Protein Analysis

556

Amino Acids

63.35

Weight (kDa)

6.6

Isoelectric Point (pI)

53.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 41 - 534 6.2e-106 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 98
AccI GTMKAC 1 cut(s) 1589
AciI CCGC 1 cut(s) 5
AclWI GGATC 5 cut(s) 78, 782, 818, 1327, 1407
AcsI RAATTY 3 cut(s) 87, 334, 418
AdeI CACNNNGTG 1 cut(s) 554
AfaI GTAC 5 cut(s) 19, 1001, 1505, 1558, 1567
AfiI CCNNNNNNNGG 3 cut(s) 98, 314, 1541
AflII CTTAAG 1 cut(s) 1313
AflIII ACRYGT 1 cut(s) 204
AgeI ACCGGT 1 cut(s) 47
AgsI TTSAA 8 cut(s) 483, 603, 902, 986, 1232, 1366, 1469, 1665
AjnI CCWGG 2 cut(s) 1522, 1540
AjuI GAANNNNNNNTTGG 2 cut(s) 1612, 1644
AluBI AGCT 9 cut(s) 400, 493, 574, 917, 925, 989, 1244, 1252, 1350
AluI AGCT 9 cut(s) 400, 493, 574, 917, 925, 989, 1244, 1252, 1350
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 5 cut(s) 78, 782, 818, 1327, 1407
Ama87I CYCGRG 1 cut(s) 1088
ApeKI GCWGC 3 cut(s) 493, 917, 1388
ApoI RAATTY 3 cut(s) 87, 334, 418
ArsI GACNNNNNNTTYG 2 cut(s) 1097, 1129
AsiGI ACCGGT 1 cut(s) 47
AsiSI GCGATCGC 1 cut(s) 1310
Asp700I GAANNNNTTC 2 cut(s) 236, 345
AspS9I GGNCC 3 cut(s) 1105, 1155, 1526
AsuHPI GGTGA 3 cut(s) 246, 419, 1421
AsuII TTCGAA 2 cut(s) 121, 1081
AvaI CYCGRG 1 cut(s) 1088
AvaII GGWCC 3 cut(s) 1105, 1155, 1526
BaeI ACNNNNGTAYC 2 cut(s) 285, 318
BanII GRGCYC 1 cut(s) 1642
BauI CACGAG 1 cut(s) 552
BbsI GAAGAC 2 cut(s) 118, 1382
BbvI GCAGC 3 cut(s) 480, 904, 1400
BccI CCATC 4 cut(s) 737, 938, 1447, 1649
BceAI ACGGC 2 cut(s) 26, 124
BciT130I CCWGG 2 cut(s) 1524, 1542
BciVI GTATCC 1 cut(s) 1159
BclI TGATCA 2 cut(s) 376, 676
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 3 cut(s) 771, 867, 1245
BfmI CTRYAG 1 cut(s) 537
BfrI CTTAAG 1 cut(s) 1313
BfuI GTATCC 1 cut(s) 1159
BisI GCNGC 3 cut(s) 494, 918, 1389
BlsI GCNGC 3 cut(s) 495, 919, 1390
BmcAI AGTACT 1 cut(s) 1505
Bme1390I CCNGG 2 cut(s) 1524, 1542
Bme18I GGWCC 3 cut(s) 1105, 1155, 1526
BmeT110I CYCGRG 1 cut(s) 1088
BmgT120I GGNCC 3 cut(s) 1105, 1155, 1526
BmiI GGNNCC 4 cut(s) 132, 219, 768, 1129
BmrFI CCNGG 2 cut(s) 1524, 1542
BmsI GCATC 3 cut(s) 703, 853, 1111
BpiI GAAGAC 2 cut(s) 118, 1382
BplI GAGNNNNNCTC 2 cut(s) 1543, 1575
BpmI CTGGAG 1 cut(s) 1118
Bpu10I CCTNAGC 1 cut(s) 1536
Bpu14I TTCGAA 2 cut(s) 121, 1081
BpuEI CTTGAG 2 cut(s) 857, 1343
BsaAI YACGTR 1 cut(s) 311
BsaBI GATNNNNATC 1 cut(s) 822
BsaJI CCNNGG 3 cut(s) 519, 1087, 1131
BsaWI WCCGGW 1 cut(s) 47
BsaXI ACNNNNNCTCC 6 cut(s) 7, 37, 875, 905, 1409, 1439
Bsc4I CCNNNNNNNGG 3 cut(s) 98, 314, 1541
Bse118I RCCGGY 1 cut(s) 47
Bse1I ACTGG 4 cut(s) 531, 784, 1101, 1348
Bse3DI GCAATG 2 cut(s) 134, 955
Bse8I GATNNNNATC 1 cut(s) 822
BseBI CCWGG 2 cut(s) 1524, 1542
BseDI CCNNGG 3 cut(s) 519, 1087, 1131
BseGI GGATG 5 cut(s) 154, 535, 748, 1141, 1504
BseJI GATNNNNATC 1 cut(s) 822
BseLI CCNNNNNNNGG 3 cut(s) 98, 314, 1541
BseMI GCAATG 2 cut(s) 134, 955
BseMII CTCAG 6 cut(s) 783, 1142, 1212, 1527, 1574, 1598
BseNI ACTGG 4 cut(s) 531, 784, 1101, 1348
BseRI GAGGAG 2 cut(s) 410, 1430
BseXI GCAGC 3 cut(s) 480, 904, 1400
BsgI GTGCAG 1 cut(s) 903
Bsh1285I CGRYCG 1 cut(s) 1310
BshTI ACCGGT 1 cut(s) 47
BsiEI CGRYCG 1 cut(s) 1310
BsiHKCI CYCGRG 1 cut(s) 1088
BsiSI CCGG 1 cut(s) 48
BslFI GGGAC 4 cut(s) 980, 1089, 1250, 1647
BslI CCNNNNNNNGG 3 cut(s) 98, 314, 1541
BsmAI GTCTC 1 cut(s) 15
BsmFI GGGAC 4 cut(s) 980, 1089, 1250, 1647
BsmI GAATGC 1 cut(s) 1645
BsoBI CYCGRG 1 cut(s) 1088
Bsp119I TTCGAA 2 cut(s) 121, 1081
Bsp1286I GDGCHC 1 cut(s) 1642
Bsp143I GATC 9 cut(s) 70, 376, 676, 787, 823, 1307, 1319, 1399, 1610
BspACI CCGC 1 cut(s) 5
BspCNI CTCAG 6 cut(s) 784, 1143, 1213, 1528, 1573, 1597
BspLI GGNNCC 4 cut(s) 132, 219, 768, 1129
BspMAI CTGCAG 1 cut(s) 541
BspPI GGATC 5 cut(s) 78, 782, 818, 1327, 1407
BspT104I TTCGAA 2 cut(s) 121, 1081
BspTI CTTAAG 1 cut(s) 1313
BsrDI GCAATG 2 cut(s) 134, 955
BsrFI RCCGGY 1 cut(s) 47
BsrI ACTGG 4 cut(s) 531, 784, 1101, 1348
BssAI RCCGGY 1 cut(s) 47
BssECI CCNNGG 3 cut(s) 519, 1087, 1131
BssMI GATC 9 cut(s) 70, 376, 676, 787, 823, 1307, 1319, 1399, 1610
BssSI CACGAG 1 cut(s) 552
BssT1I CCWWGG 2 cut(s) 519, 1131
Bst2BI CACGAG 1 cut(s) 552
Bst2UI CCWGG 2 cut(s) 1524, 1542
Bst4CI ACNGT 4 cut(s) 430, 472, 673, 999
Bst6I CTCTTC 1 cut(s) 1407
BstAFI CTTAAG 1 cut(s) 1313
BstAPI GCANNNNNTGC 1 cut(s) 63
BstBAI YACGTR 1 cut(s) 311
BstBI TTCGAA 2 cut(s) 121, 1081
BstC8I GCNNGC 1 cut(s) 576
BstDEI CTNAG 7 cut(s) 423, 792, 1151, 1221, 1536, 1560, 1584
BstENI CCTNNNNNAGG 1 cut(s) 1539
BstF5I GGATG 5 cut(s) 154, 535, 748, 1141, 1504
BstKTI GATC 9 cut(s) 73, 379, 679, 790, 826, 1310, 1322, 1402, 1613
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 9 cut(s) 70, 376, 676, 787, 823, 1307, 1319, 1399, 1610
BstMCI CGRYCG 1 cut(s) 1310
BstMWI GCNNNNNNNGC 4 cut(s) 63, 502, 521, 1099
BstNI CCWGG 2 cut(s) 1524, 1542
BstNSI RCATGY 3 cut(s) 208, 648, 1306
BstSCI CCNGG 2 cut(s) 1522, 1540
BstSFI CTRYAG 1 cut(s) 537
BstV1I GCAGC 3 cut(s) 480, 904, 1400
BstV2I GAAGAC 2 cut(s) 118, 1382
BstX2I RGATCY 1 cut(s) 787
BstXI CCANNNNNNTGG 1 cut(s) 526
BstYI RGATCY 1 cut(s) 787
BsuI GTATCC 1 cut(s) 1159
BtsCI GGATG 5 cut(s) 154, 535, 748, 1141, 1504
BtsIMutI CAGTG 4 cut(s) 372, 524, 1002, 1448
Cac8I GCNNGC 1 cut(s) 576
Cfr10I RCCGGY 1 cut(s) 47
Cfr13I GGNCC 3 cut(s) 1105, 1155, 1526
CsiI ACCWGGT 1 cut(s) 1522
Csp6I GTAC 5 cut(s) 18, 1000, 1504, 1557, 1566
CspAI ACCGGT 1 cut(s) 47
CspCI CAANNNNNGTGG 2 cut(s) 763, 798
CviAII CATG 9 cut(s) 190, 205, 645, 761, 1031, 1277, 1285, 1303, 1509
CviQI GTAC 5 cut(s) 18, 1000, 1504, 1557, 1566
DdeI CTNAG 7 cut(s) 423, 792, 1151, 1221, 1536, 1560, 1584
DpnI GATC 9 cut(s) 72, 378, 678, 789, 825, 1309, 1321, 1401, 1612
DpnII GATC 9 cut(s) 70, 376, 676, 787, 823, 1307, 1319, 1399, 1610
DraIII CACNNNGTG 1 cut(s) 554
Eam1104I CTCTTC 1 cut(s) 1407
EarI CTCTTC 1 cut(s) 1407
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 519, 1131
Eco24I GRGCYC 1 cut(s) 1642
Eco32I GATATC 1 cut(s) 509
Eco47I GGWCC 3 cut(s) 1105, 1155, 1526
Eco88I CYCGRG 1 cut(s) 1088
EcoNI CCTNNNNNAGG 1 cut(s) 1539
EcoO109I RGGNCCY 1 cut(s) 1105
EcoRI GAATTC 2 cut(s) 87, 418
EcoRII CCWGG 2 cut(s) 1522, 1540
EcoRV GATATC 1 cut(s) 509
EcoT14I CCWWGG 2 cut(s) 519, 1131
EcoT38I GRGCYC 1 cut(s) 1642
ErhI CCWWGG 2 cut(s) 519, 1131
FaeI CATG 9 cut(s) 193, 208, 648, 764, 1034, 1280, 1288, 1306, 1512
FalI AAGNNNNNCTT 4 cut(s) 205, 237, 791, 823
FaqI GGGAC 4 cut(s) 980, 1089, 1250, 1647
FatI CATG 9 cut(s) 189, 204, 644, 760, 1030, 1276, 1284, 1302, 1508
FauNDI CATATG 2 cut(s) 499, 709
FbaI TGATCA 2 cut(s) 376, 676
FblI GTMKAC 1 cut(s) 1589
Fnu4HI GCNGC 3 cut(s) 494, 918, 1389
FokI GGATG 5 cut(s) 161, 542, 755, 1148, 1511
FriOI GRGCYC 1 cut(s) 1642
Fsp4HI GCNGC 3 cut(s) 494, 918, 1389
FspBI CTAG 3 cut(s) 771, 867, 1245
GluI GCNGC 3 cut(s) 494, 918, 1389
GsuI CTGGAG 1 cut(s) 1118
HapII CCGG 1 cut(s) 48
Hin1II CATG 9 cut(s) 193, 208, 648, 764, 1034, 1280, 1288, 1306, 1512
HincII GTYRAC 2 cut(s) 1581, 1590
HindII GTYRAC 2 cut(s) 1581, 1590
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 4 cut(s) 8, 533, 841, 1328
HpaII CCGG 1 cut(s) 48
HphI GGTGA 3 cut(s) 246, 419, 1421
Hpy166II GTNNAC 5 cut(s) 254, 872, 1002, 1581, 1590
Hpy188I TCNGA 9 cut(s) 15, 202, 681, 1152, 1327, 1439, 1563, 1573, 1651
Hpy8I GTNNAC 5 cut(s) 254, 872, 1002, 1581, 1590
HpyAV CCTTC 6 cut(s) 117, 207, 481, 1226, 1282, 1400
HpyCH4III ACNGT 4 cut(s) 430, 472, 673, 999
HpyCH4IV ACGT 2 cut(s) 310, 1424
HpyF10VI GCNNNNNNNGC 4 cut(s) 63, 502, 521, 1099
HpyF3I CTNAG 7 cut(s) 423, 792, 1151, 1221, 1536, 1560, 1584
HpySE526I ACGT 2 cut(s) 310, 1424
Hsp92II CATG 9 cut(s) 193, 208, 648, 764, 1034, 1280, 1288, 1306, 1512
Ksp22I TGATCA 2 cut(s) 376, 676
Kzo9I GATC 9 cut(s) 70, 376, 676, 787, 823, 1307, 1319, 1399, 1610
LmnI GCTCC 8 cut(s) 130, 217, 397, 490, 691, 930, 1099, 1127
Lsp1109I GCAGC 3 cut(s) 480, 904, 1400
LweI GCATC 3 cut(s) 703, 853, 1111
MabI ACCWGGT 1 cut(s) 1522
MaeI CTAG 3 cut(s) 771, 867, 1245
MaeII ACGT 2 cut(s) 310, 1424
MaeIII GTNAC 3 cut(s) 43, 722, 881
MalI GATC 9 cut(s) 72, 378, 678, 789, 825, 1309, 1321, 1401, 1612
MboI GATC 9 cut(s) 70, 376, 676, 787, 823, 1307, 1319, 1399, 1610
MboII GAAGA 5 cut(s) 123, 606, 827, 1387, 1424
MflI RGATCY 1 cut(s) 787
MhlI GDGCHC 1 cut(s) 1642
MluCI AATT 8 cut(s) 87, 334, 418, 462, 561, 953, 1144, 1627
MlyI GAGTC 1 cut(s) 17
MmeI TCCRAC 2 cut(s) 952, 1553
MroXI GAANNNNTTC 2 cut(s) 236, 345
MseI TTAA 4 cut(s) 560, 1053, 1314, 1476
MslI CAYNNNNRTG 1 cut(s) 1650
MspCI CTTAAG 1 cut(s) 1313
MspI CCGG 1 cut(s) 48
MspR9I CCNGG 2 cut(s) 1524, 1542
Mva1269I GAATGC 1 cut(s) 1645
MvaI CCWGG 2 cut(s) 1524, 1542
MwoI GCNNNNNNNGC 4 cut(s) 63, 502, 521, 1099
NdeI CATATG 2 cut(s) 499, 709
NdeII GATC 9 cut(s) 70, 376, 676, 787, 823, 1307, 1319, 1399, 1610
NlaIII CATG 9 cut(s) 193, 208, 648, 764, 1034, 1280, 1288, 1306, 1512
NlaIV GGNNCC 4 cut(s) 132, 219, 768, 1129
NmeAIII GCCGAG 1 cut(s) 1621
NmuCI GTSAC 3 cut(s) 43, 722, 881
NspI RCATGY 3 cut(s) 208, 648, 1306
NspV TTCGAA 2 cut(s) 121, 1081
PciI ACATGT 1 cut(s) 204
PctI GAATGC 1 cut(s) 1645
PdmI GAANNNNTTC 2 cut(s) 236, 345
PfeI GAWTC 3 cut(s) 533, 841, 1328
PflMI CCANNNNNTGG 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 47
PkrI GCNGC 3 cut(s) 495, 919, 1390
Ple19I CGATCG 1 cut(s) 1310
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
Ppu21I YACGTR 1 cut(s) 311
PpuMI RGGWCCY 1 cut(s) 1105
PscI ACATGT 1 cut(s) 204
Psp5II RGGWCCY 1 cut(s) 1105
Psp6I CCWGG 2 cut(s) 1522, 1540
PspGI CCWGG 2 cut(s) 1522, 1540
PspN4I GGNNCC 4 cut(s) 132, 219, 768, 1129
PspPI GGNCC 3 cut(s) 1105, 1155, 1526
PspPPI RGGWCCY 1 cut(s) 1105
PstI CTGCAG 1 cut(s) 541
PsuI RGATCY 1 cut(s) 787
PvuI CGATCG 1 cut(s) 1310
RgaI GCGATCGC 1 cut(s) 1310
RsaI GTAC 5 cut(s) 19, 1001, 1505, 1558, 1567
RsaNI GTAC 5 cut(s) 18, 1000, 1504, 1557, 1566
RseI CAYNNNNRTG 1 cut(s) 1650
SalI GTCGAC 1 cut(s) 1588
SaqAI TTAA 4 cut(s) 560, 1053, 1314, 1476
SatI GCNGC 3 cut(s) 494, 918, 1389
Sau3AI GATC 9 cut(s) 70, 376, 676, 787, 823, 1307, 1319, 1399, 1610
Sau96I GGNCC 3 cut(s) 1105, 1155, 1526
ScaI AGTACT 1 cut(s) 1505
SchI GAGTC 1 cut(s) 17
ScrFI CCNGG 2 cut(s) 1524, 1542
SduI GDGCHC 1 cut(s) 1642
SexAI ACCWGGT 1 cut(s) 1522
SfaAI GCGATCGC 1 cut(s) 1310
SfaNI GCATC 3 cut(s) 703, 853, 1111
SfcI CTRYAG 1 cut(s) 537
SfuI TTCGAA 2 cut(s) 121, 1081
SgfI GCGATCGC 1 cut(s) 1310
SinI GGWCC 3 cut(s) 1105, 1155, 1526
SmiMI CAYNNNNRTG 1 cut(s) 1650
SmlI CTYRAG 3 cut(s) 836, 1313, 1358
SmoI CTYRAG 3 cut(s) 836, 1313, 1358
Sse9I AATT 8 cut(s) 87, 334, 418, 462, 561, 953, 1144, 1627
SsiI CCGC 1 cut(s) 5
SspI AATATT 1 cut(s) 184
SspMI CTAG 3 cut(s) 771, 867, 1245
StyD4I CCNGG 2 cut(s) 1522, 1540
StyI CCWWGG 2 cut(s) 519, 1131
TaaI ACNGT 4 cut(s) 430, 472, 673, 999
TaiI ACGT 2 cut(s) 313, 1427
TaqI TCGA 6 cut(s) 121, 432, 460, 1081, 1589, 1604
TasI AATT 8 cut(s) 87, 334, 418, 462, 561, 953, 1144, 1627
TatI WGTACW 4 cut(s) 17, 999, 1503, 1565
TfiI GAWTC 3 cut(s) 533, 841, 1328
Tru1I TTAA 4 cut(s) 560, 1053, 1314, 1476
Tru9I TTAA 4 cut(s) 560, 1053, 1314, 1476
TscAI CASTG 4 cut(s) 379, 531, 1009, 1455
TseFI GTSAC 3 cut(s) 43, 722, 881
TseI GCWGC 3 cut(s) 493, 917, 1388
Tsp45I GTSAC 3 cut(s) 43, 722, 881
TspDTI ATGAA 8 cut(s) 229, 347, 500, 546, 966, 1301, 1529, 1533
TspGWI ACGGA 2 cut(s) 19, 97
TspRI CASTG 4 cut(s) 379, 531, 1009, 1455
Van91I CCANNNNNTGG 1 cut(s) 98
Vha464I CTTAAG 1 cut(s) 1313
VpaK11BI GGWCC 3 cut(s) 1105, 1155, 1526
XagI CCTNNNNNAGG 1 cut(s) 1539
XapI RAATTY 3 cut(s) 87, 334, 418
XceI RCATGY 3 cut(s) 208, 648, 1306
XmiI GTMKAC 1 cut(s) 1589
XmnI GAANNNNTTC 2 cut(s) 236, 345
XspI CTAG 3 cut(s) 771, 867, 1245
ZrmI AGTACT 1 cut(s) 1505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.