MD07G1121500.v1.1

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005583
Physical Location & Seq
Reverse (-)
15536544 .. 15542477
5934 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1121500.v1.1.491

Sequence Viewer

Length: 2010 bp
ATGTCGTTTTCTGATTCTGATTCGTCTTCTCATGGCGGAGCTACGGAGTACAAAATTTTCCGACAAGTCAGCCGTGACCGGTTATTGCACGAAATGCTTGGATCTACCAGAACAGAGAATTCTAAGTCTTGGAAGGTGCTTATCATGGATAAAGTGACAGTGAAAGTTATGTCCCATTCATGTAAAATGGCAGATATCACAGACCAAGAAATTTCGCTGGTGGAAGACCTTTTTCGGAGGAGAGAACCATTGCCGTCCATGGATGTTGTTTATTTCATCCAGCCAACAAAAGAGAATATAGTCATGTTCCTGTCTGACATGTCTGGAAGGGAGCCGTTATACAGGAAAGCATTCGTATTTTTCAGTTCACCTATCCCAAAGGAACTTGTAAATCACATAAAGAGCGATACAAGTGTTTTACCCCGCATAGGTGCACTGAGAGAGATGAATTTTGAGTACTTTCCTATAGATAGTCAGGCTTTTGTCACTGATCAACAAAGGGCGTTGGAGGAGCTCTTCGGTAATACTGAGAATGCTCCCAAATTCAATGCTTCCATAAACATAATGGCAACTCGAATTGCTACGGTTTTTGCTTCTTTGAAGGAGCTCCCATATGTGCGTTATCATGCTGCCCAGTCAGATGACTCAACAGAAGCATCACCTCGTGACTTAATTCCTACAAAGCTTGCTGCTGCTGTTTGGGACATAATTTCAAATTATAAATCTTCTATTCCCAACTTTCCGCAGAAAGAGACATGCGAACTGCTCATCTTGGATAGATCTGTTGATCAGATTGCTCCGGTCATACATGAATGGACATACGACGCTATGTGTCACGATTTATTGGATATGGACGGGAACAAATATATGCATGAGGTTCCTAGCAAAACTGGTGGCGATCCTGAGAGAAAGGAGGTGCTTTTGGAAGACAACGATCCAGTCTGGCTTGAGCTTCGTCATACACACATAGCAGATGCTAGCGAACGGTTGAGCGACAAGTTTACCACCTTTCAATCAAAGAATAAAGCTGCACAGCTCCAACAGAGTGCAAGAGAGGGCAATGAGTTATCTACGCGGGACTTGCAGAAGATGGTTCAAGCTTTGCCACAATACACTGAACAAGTTGAAAAGATTTCTCTCCACGTTGAGATTGCAGGGAAAATTAACAAAATTATCAGGGAAACAGGACTTCGAGACCTCGGGCAACTGGAGCAGGATCTTGTTTTTGGAGATGCAGGAGCCAAGGATGTCATCAATTATCTGAGGACAAATCAGGATACAACGCCTGAAAATAAGTTGCGTCTTTTAATGATTTATGCATCTGTATATCCTGAGAAGTTCGAGGGAGACAAAGCTAATAAGCTAATGCAGTTGGCAAAATTATCACGGGAGGACATGAAGGTTGTGAATAATATGCGGTTGCTTGGGGGTTCATCAGAGTCCAAGAAGACATCTAGTAGCTTCTCACTGAAGTTCAATGCTGCGAAGACGAAGGGAGCAGCGAGGAAGGATCGTACTGATGAGGTGGAAACATGGCAACTATTTCGATTCTATCCCATGATAGAGGAGATCATTGAAAATCTTAGTAAAGGTGAATTACCAAAGAGCGAGTATTCATGCTTGAATGAACCTACTCCGGCTCCCCAAGGGGGCAGTGTGAGAGGCCACAGTTCATCAGCGCAGACAAGCCAATCTACCGGTGCTCCTCATTCAATGAGATCAAGGCGGACTGCTAACTGGGGACGGGCTCGGCATTCTGATGATGGATATTCAAGTGACTCAGTTTTGAGAGGTTCATCTACTGATTTCAAGAAGATGGGGCAACGTATCTTTGTATTTATGATTGGTGGGGCAACTCGATCTGAGTTACGAGTTTGTCACAAGCTAACGACGAAACTGAGAAGGGAAGTAATCCTGGGTACTACTGGTCTCATTGAACCCCCACAGTATATTACGAAACTGAAGCTGCTATCAGAAAAAGAGCTAGCAATGGACGGCCTTGGAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 map04721
Pfam Domains
Protein Families
Sec1

Protein Analysis

670

Amino Acids

75.63

Weight (kDa)

6.61

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 45 - 652 2.8e-133 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 720
AccII CGCG 1 cut(s) 1075
AciI CCGC 6 cut(s) 36, 424, 743, 1075, 1417, 1726
AclWI GGATC 5 cut(s) 109, 893, 929, 1224, 1518
AcsI RAATTY 5 cut(s) 54, 118, 210, 448, 542
AcuI CTGAAG 2 cut(s) 1490, 1982
AdeI CACNNNGTG 1 cut(s) 665
AfaI GTAC 4 cut(s) 50, 458, 1516, 1921
AfiI CCNNNNNNNGG 2 cut(s) 428, 1649
AflIII ACRYGT 1 cut(s) 318
AgeI ACCGGT 2 cut(s) 78, 1697
AjnI CCWGG 1 cut(s) 1914
Alw21I GWGCWC 4 cut(s) 436, 516, 609, 1705
Alw26I GTCTC 4 cut(s) 746, 1188, 1341, 1934
Alw44I GTGCAC 1 cut(s) 432
AlwI GGATC 5 cut(s) 109, 893, 929, 1224, 1518
Ama87I CYCGRG 1 cut(s) 1199
AoxI GGCC 2 cut(s) 1663, 1996
ApaLI GTGCAC 1 cut(s) 432
ApeKI GCWGC 7 cut(s) 629, 689, 692, 1028, 1481, 1499, 1966
ApoI RAATTY 5 cut(s) 54, 118, 210, 448, 542
AsiGI ACCGGT 2 cut(s) 78, 1697
Asp700I GAANNNNTTC 1 cut(s) 350
AspLEI GCGC 1 cut(s) 1681
AsuHPI GGTGA 3 cut(s) 360, 651, 1604
AsuNHI GCTAGC 2 cut(s) 977, 1984
AvaI CYCGRG 1 cut(s) 1199
BaeGI GKGCMC 1 cut(s) 436
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BanII GRGCYC 3 cut(s) 516, 609, 1750
BauI CACGAG 1 cut(s) 663
BbsI GAAGAC 5 cut(s) 18, 231, 933, 1454, 1493
Bbv12I GWGCWC 4 cut(s) 436, 516, 609, 1705
BbvI GCAGC 7 cut(s) 616, 676, 679, 1015, 1468, 1511, 1953
BccI CCATC 3 cut(s) 1084, 1757, 1810
BceAI ACGGC 3 cut(s) 57, 238, 319
BcgI CGANNNNNNTGC 2 cut(s) 1526, 1560
BciT130I CCWGG 1 cut(s) 1916
BciVI GTATCC 1 cut(s) 1270
BclI TGATCA 2 cut(s) 490, 787
BcoDI GTCTC 4 cut(s) 746, 1188, 1341, 1934
BfaI CTAG 4 cut(s) 882, 978, 1455, 1985
BfmI CTRYAG 1 cut(s) 465
BfuI GTATCC 1 cut(s) 1270
BglII AGATCT 1 cut(s) 779
BisI GCNGC 7 cut(s) 630, 690, 693, 1029, 1482, 1500, 1967
BlsI GCNGC 7 cut(s) 631, 691, 694, 1030, 1483, 1501, 1968
BmcAI AGTACT 1 cut(s) 458
Bme1390I CCNGG 1 cut(s) 1916
BmeT110I CYCGRG 1 cut(s) 1199
BmiI GGNNCC 4 cut(s) 333, 879, 1240, 1641
BmrFI CCNGG 1 cut(s) 1916
BmrI ACTGGG 2 cut(s) 628, 1747
BmsI GCATC 4 cut(s) 665, 964, 1222, 1328
BmtI GCTAGC 2 cut(s) 981, 1988
BmuI ACTGGG 2 cut(s) 628, 1747
BpiI GAAGAC 5 cut(s) 18, 231, 933, 1454, 1493
BpmI CTGGAG 1 cut(s) 1229
BpuEI CTTGAG 1 cut(s) 968
BsaI GGTCTC 2 cut(s) 1188, 1934
BsaJI CCNNGG 6 cut(s) 258, 1198, 1242, 1645, 1915, 1999
BsaWI WCCGGW 3 cut(s) 78, 799, 1697
BsaXI ACNNNNNCTCC 4 cut(s) 1624, 1654, 1687, 1717
Bsc4I CCNNNNNNNGG 2 cut(s) 428, 1649
Bse118I RCCGGY 2 cut(s) 78, 1697
Bse1I ACTGG 6 cut(s) 634, 895, 938, 1212, 1742, 1930
Bse3DI GCAATG 3 cut(s) 248, 1066, 1995
BseBI CCWGG 1 cut(s) 1916
BseDI CCNNGG 6 cut(s) 258, 1198, 1242, 1645, 1915, 1999
BseGI GGATG 3 cut(s) 268, 276, 1252
BseLI CCNNNNNNNGG 2 cut(s) 428, 1649
BseMI GCAATG 3 cut(s) 248, 1066, 1995
BseMII CTCAG 8 cut(s) 428, 519, 894, 1253, 1323, 1794, 1854, 1889
BseNI ACTGG 6 cut(s) 634, 895, 938, 1212, 1742, 1930
BseRI GAGGAG 4 cut(s) 253, 524, 1580, 1695
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 7 cut(s) 616, 676, 679, 1015, 1468, 1511, 1953
BsgI GTGCAG 1 cut(s) 1014
Bsh1236I CGCG 1 cut(s) 1075
BshFI GGCC 2 cut(s) 1665, 1998
BshTI ACCGGT 2 cut(s) 78, 1697
BsiHKAI GWGCWC 4 cut(s) 436, 516, 609, 1705
BsiHKCI CYCGRG 1 cut(s) 1199
BsiSI CCGG 4 cut(s) 79, 800, 1637, 1698
BslFI GGGAC 4 cut(s) 157, 716, 1091, 1755
BslI CCNNNNNNNGG 2 cut(s) 428, 1649
BsmAI GTCTC 4 cut(s) 746, 1188, 1341, 1934
BsmFI GGGAC 4 cut(s) 157, 716, 1091, 1755
BsmI GAATGC 3 cut(s) 350, 538, 1753
BsnI GGCC 2 cut(s) 1665, 1998
Bso31I GGTCTC 2 cut(s) 1188, 1934
BsoBI CYCGRG 1 cut(s) 1199
Bsp1286I GDGCHC 5 cut(s) 436, 516, 609, 1705, 1750
Bsp19I CCATGG 1 cut(s) 258
BspACI CCGC 6 cut(s) 36, 424, 743, 1075, 1417, 1726
BspANI GGCC 2 cut(s) 1665, 1998
BspCNI CTCAG 8 cut(s) 429, 520, 895, 1254, 1324, 1793, 1855, 1890
BspFNI CGCG 1 cut(s) 1075
BspLI GGNNCC 4 cut(s) 333, 879, 1240, 1641
BspOI GCTAGC 2 cut(s) 981, 1988
BspPI GGATC 5 cut(s) 109, 893, 929, 1224, 1518
BspQI GCTCTTC 1 cut(s) 521
BspTNI GGTCTC 2 cut(s) 1188, 1934
BsrDI GCAATG 3 cut(s) 248, 1066, 1995
BsrFI RCCGGY 2 cut(s) 78, 1697
BsrI ACTGG 6 cut(s) 634, 895, 938, 1212, 1742, 1930
BssAI RCCGGY 2 cut(s) 78, 1697
BssECI CCNNGG 6 cut(s) 258, 1198, 1242, 1645, 1915, 1999
BssSI CACGAG 1 cut(s) 663
BssT1I CCWWGG 4 cut(s) 258, 1242, 1645, 1999
Bst2BI CACGAG 1 cut(s) 663
Bst2UI CCWGG 1 cut(s) 1916
Bst4CI ACNGT 5 cut(s) 160, 586, 987, 1670, 1947
Bst6I CTCTTC 1 cut(s) 521
BstAPI GCANNNNNTGC 1 cut(s) 94
BstC8I GCNNGC 3 cut(s) 687, 979, 1986
BstDSI CCRYGG 1 cut(s) 258
BstF5I GGATG 3 cut(s) 268, 276, 1252
BstFNI CGCG 1 cut(s) 1075
BstHHI GCGC 1 cut(s) 1681
BstMAI GTCTC 4 cut(s) 746, 1188, 1341, 1934
BstMWI GCNNNNNNNGC 3 cut(s) 94, 1081, 1210
BstNI CCWGG 1 cut(s) 1916
BstNSI RCATGY 2 cut(s) 322, 759
BstSCI CCNGG 1 cut(s) 1914
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 436
BstUI CGCG 1 cut(s) 1075
BstV1I GCAGC 7 cut(s) 616, 676, 679, 1015, 1468, 1511, 1953
BstV2I GAAGAC 5 cut(s) 18, 231, 933, 1454, 1493
BstX2I RGATCY 3 cut(s) 101, 779, 1216
BstYI RGATCY 3 cut(s) 101, 779, 1216
BsuI GTATCC 1 cut(s) 1270
BsuRI GGCC 2 cut(s) 1665, 1998
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 3 cut(s) 268, 276, 1252
BtsI GCAGTG 1 cut(s) 1660
BtsIMutI CAGTG 6 cut(s) 165, 434, 486, 1113, 1466, 1660
Cac8I GCNNGC 3 cut(s) 687, 979, 1986
CfoI GCGC 1 cut(s) 1681
Cfr10I RCCGGY 2 cut(s) 78, 1697
CseI GACGC 2 cut(s) 833, 1289
Csp6I GTAC 4 cut(s) 49, 457, 1515, 1920
CspAI ACCGGT 2 cut(s) 78, 1697
CspCI CAANNNNNGTGG 2 cut(s) 874, 909
CviQI GTAC 4 cut(s) 49, 457, 1515, 1920
DraIII CACNNNGTG 1 cut(s) 665
Eam1104I CTCTTC 1 cut(s) 521
EarI CTCTTC 1 cut(s) 521
EciI GGCGGA 2 cut(s) 51, 1741
Ecl136II GAGCTC 2 cut(s) 514, 607
Eco130I CCWWGG 4 cut(s) 258, 1242, 1645, 1999
Eco24I GRGCYC 3 cut(s) 516, 609, 1750
Eco31I GGTCTC 2 cut(s) 1188, 1934
Eco32I GATATC 1 cut(s) 196
Eco53kI GAGCTC 2 cut(s) 514, 607
Eco57I CTGAAG 2 cut(s) 1490, 1982
Eco88I CYCGRG 1 cut(s) 1199
EcoICRI GAGCTC 2 cut(s) 514, 607
EcoRI GAATTC 1 cut(s) 118
EcoRII CCWGG 1 cut(s) 1914
EcoRV GATATC 1 cut(s) 196
EcoT14I CCWWGG 4 cut(s) 258, 1242, 1645, 1999
EcoT22I ATGCAT 2 cut(s) 873, 1321
EcoT38I GRGCYC 3 cut(s) 516, 609, 1750
ErhI CCWWGG 4 cut(s) 258, 1242, 1645, 1999
FaqI GGGAC 4 cut(s) 157, 716, 1091, 1755
FauI CCCGC 2 cut(s) 431, 1068
FauNDI CATATG 1 cut(s) 613
FbaI TGATCA 2 cut(s) 490, 787
Fnu4HI GCNGC 7 cut(s) 630, 690, 693, 1029, 1482, 1500, 1967
FokI GGATG 3 cut(s) 263, 275, 1259
FriOI GRGCYC 3 cut(s) 516, 609, 1750
Fsp4HI GCNGC 7 cut(s) 630, 690, 693, 1029, 1482, 1500, 1967
FspBI CTAG 4 cut(s) 882, 978, 1455, 1985
GlaI GCGC 1 cut(s) 1680
GluI GCNGC 7 cut(s) 630, 690, 693, 1029, 1482, 1500, 1967
GsuI CTGGAG 1 cut(s) 1229
HaeIII GGCC 2 cut(s) 1665, 1998
HapII CCGG 4 cut(s) 79, 800, 1637, 1698
HgaI GACGC 2 cut(s) 833, 1289
HhaI GCGC 1 cut(s) 1681
Hin6I GCGC 1 cut(s) 1679
HinP1I GCGC 1 cut(s) 1679
HindIII AAGCTT 2 cut(s) 683, 1098
HinfI GANTC 7 cut(s) 14, 20, 644, 1439, 1548, 1778, 2004
HpaII CCGG 4 cut(s) 79, 800, 1637, 1698
HphI GGTGA 3 cut(s) 360, 651, 1604
Hpy166II GTNNAC 3 cut(s) 368, 434, 1002
Hpy188III TCNNGA 8 cut(s) 324, 665, 836, 902, 1193, 1274, 1331, 1810
Hpy8I GTNNAC 3 cut(s) 368, 434, 1002
Hpy99I CGWCG 2 cut(s) 827, 1894
HpyAV CCTTC 7 cut(s) 127, 321, 595, 1393, 1486, 1501, 1896
HpyCH4III ACNGT 5 cut(s) 160, 586, 987, 1670, 1947
HpyCH4IV ACGT 2 cut(s) 1143, 1825
HpyF10VI GCNNNNNNNGC 3 cut(s) 94, 1081, 1210
HpySE526I ACGT 2 cut(s) 1143, 1825
HspAI GCGC 1 cut(s) 1679
Ksp22I TGATCA 2 cut(s) 490, 787
LguI GCTCTTC 1 cut(s) 521
Lsp1109I GCAGC 7 cut(s) 616, 676, 679, 1015, 1468, 1511, 1953
LweI GCATC 4 cut(s) 665, 964, 1222, 1328
MaeI CTAG 4 cut(s) 882, 978, 1455, 1985
MaeII ACGT 2 cut(s) 1143, 1825
MaeIII GTNAC 8 cut(s) 74, 154, 484, 665, 833, 1775, 1866, 1877
MboII GAAGA 9 cut(s) 18, 236, 508, 717, 938, 1099, 1459, 1498, 1825
MflI RGATCY 3 cut(s) 101, 779, 1216
MhlI GDGCHC 5 cut(s) 436, 516, 609, 1705, 1750
MlyI GAGTC 3 cut(s) 638, 1448, 1772
MmeI TCCRAC 3 cut(s) 85, 486, 1063
Mph1103I ATGCAT 2 cut(s) 873, 1321
MroXI GAANNNNTTC 1 cut(s) 350
MseI TTAA 3 cut(s) 671, 1164, 1307
MslI CAYNNNNRTG 1 cut(s) 1758
MspI CCGG 4 cut(s) 79, 800, 1637, 1698
MspR9I CCNGG 1 cut(s) 1916
Mva1269I GAATGC 3 cut(s) 350, 538, 1753
MvaI CCWGG 1 cut(s) 1916
MvnI CGCG 1 cut(s) 1075
MwoI GCNNNNNNNGC 3 cut(s) 94, 1081, 1210
NcoI CCATGG 1 cut(s) 258
NdeI CATATG 1 cut(s) 613
NheI GCTAGC 2 cut(s) 977, 1984
NlaIV GGNNCC 4 cut(s) 333, 879, 1240, 1641
NmeAIII GCCGAG 1 cut(s) 1729
NmuCI GTSAC 7 cut(s) 74, 154, 484, 665, 833, 1775, 1877
NsiI ATGCAT 2 cut(s) 873, 1321
NspI RCATGY 2 cut(s) 322, 759
PciI ACATGT 1 cut(s) 318
PciSI GCTCTTC 1 cut(s) 521
PctI GAATGC 3 cut(s) 350, 538, 1753
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 4 cut(s) 14, 20, 1548, 2004
PinAI ACCGGT 2 cut(s) 78, 1697
PkrI GCNGC 7 cut(s) 631, 691, 694, 1030, 1483, 1501, 1968
PleI GAGTC 3 cut(s) 638, 1447, 1772
PpsI GAGTC 3 cut(s) 638, 1447, 1772
PscI ACATGT 1 cut(s) 318
PsiI TTATAA 1 cut(s) 720
Psp124BI GAGCTC 2 cut(s) 516, 609
Psp6I CCWGG 1 cut(s) 1914
PspGI CCWGG 1 cut(s) 1914
PspN4I GGNNCC 4 cut(s) 333, 879, 1240, 1641
PsuI RGATCY 3 cut(s) 101, 779, 1216
RsaI GTAC 4 cut(s) 50, 458, 1516, 1921
RsaNI GTAC 4 cut(s) 49, 457, 1515, 1920
RseI CAYNNNNRTG 1 cut(s) 1758
SacI GAGCTC 2 cut(s) 516, 609
SapI GCTCTTC 1 cut(s) 521
SaqAI TTAA 3 cut(s) 671, 1164, 1307
SatI GCNGC 7 cut(s) 630, 690, 693, 1029, 1482, 1500, 1967
ScaI AGTACT 1 cut(s) 458
SchI GAGTC 3 cut(s) 638, 1448, 1772
ScrFI CCNGG 1 cut(s) 1916
SduI GDGCHC 5 cut(s) 436, 516, 609, 1705, 1750
SfaNI GCATC 4 cut(s) 665, 964, 1222, 1328
SfcI CTRYAG 1 cut(s) 465
SmiMI CAYNNNNRTG 1 cut(s) 1758
SmlI CTYRAG 1 cut(s) 947
SmoI CTYRAG 1 cut(s) 947
SsiI CCGC 6 cut(s) 36, 424, 743, 1075, 1417, 1726
SspMI CTAG 4 cut(s) 882, 978, 1455, 1985
SstI GAGCTC 2 cut(s) 516, 609
StyD4I CCNGG 1 cut(s) 1914
StyI CCWWGG 4 cut(s) 258, 1242, 1645, 1999
TaaI ACNGT 5 cut(s) 160, 586, 987, 1670, 1947
TaiI ACGT 2 cut(s) 1146, 1828
TaqI TCGA 5 cut(s) 574, 1192, 1341, 1546, 1858
TatI WGTACW 2 cut(s) 48, 456
TfiI GAWTC 4 cut(s) 14, 20, 1548, 2004
Tru1I TTAA 3 cut(s) 671, 1164, 1307
Tru9I TTAA 3 cut(s) 671, 1164, 1307
TscAI CASTG 6 cut(s) 165, 441, 493, 1120, 1473, 1660
TseFI GTSAC 7 cut(s) 74, 154, 484, 665, 833, 1775, 1877
TseI GCWGC 7 cut(s) 629, 689, 692, 1028, 1481, 1499, 1966
Tsp45I GTSAC 7 cut(s) 74, 154, 484, 665, 833, 1775, 1877
TspGWI ACGGA 1 cut(s) 59
TspRI CASTG 6 cut(s) 165, 441, 493, 1120, 1473, 1660
VneI GTGCAC 1 cut(s) 432
XapI RAATTY 5 cut(s) 54, 118, 210, 448, 542
XceI RCATGY 2 cut(s) 322, 759
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 4 cut(s) 882, 978, 1455, 1985
ZrmI AGTACT 1 cut(s) 458
Zsp2I ATGCAT 2 cut(s) 873, 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.