Rh1AG226100

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005583
Physical Location & Seq
Forward (+)
43041925 .. 43050869
8945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG226100.1

Sequence Viewer

Length: 1785 bp
ATGTCGTTTTCAGACTCTGAATCATCTTCCCATGGCGGAGTCTCGGAGTACAAGTGTTTCCGTCAGTTCAGCCGTGACCGGTTATTGCACGAAATGCTTGGATCAACCAGAACGGAGAATTCCAAGTCTTGGAAGGTGCTCATCATGGATAAAGTGACAGTGAAAGTCATGTCCCATTCATGTAAAATGGCAGATATCACAGACCAAGAAATTTCATTGGTGGAAGACCTTTTTCGAAGGAGGGAGCCATTGCCGTCAATGGATGTTATTTATTTTATCCAGCCAACAAAAGAGAATATTGTCATGTTCTTGTCTGACATGTCTGGAAGGGAGCCTTTATACAGGAAAGCGTTCATATTTTTCAGTTCACCTATCCCAAAGGAACTTGTAAATCACATAAAGAGTGATACAAGTGTTTTACCACGTATAGGTGCATTGAGAGAGATGAATTTGGAATACTTTCCCATAGATAATCAGGCTTTTATCACTGATCAAGATAGGGCATTGGAGGAGCTTTTTGGTGATGTTGAGAATTCTAAGAGAGTCGAGAAGTGCTTGAACATAATGGCAACTCGAATTGCGACAGTTTTTGCTTCAATGAAGGAGCTGCCATATGTGCGATACCGTGCTTCCAAGGCACTGGATGAATCTGCAGAAGCAACACCTCGTGATTTAATTCCTACAAAGCTTGCTACTGCTGTTTGGGACATTCTTTCAAAATATAAATCTTCTATTCCCAACTTTCCACAGAAAGAAACATGCGACTTACTCATCTTGGATAGAACTGTTGATCAGATTGCTCCTGTCATACACGAATGGACATATGATGCTATGTGTCACGATTTATTGAATATGGATGGCAACAAATATGTGCATGAGGTTCCTAGCAAAACTGGTGGAGATCCTGAGAGAAAGGAGGTCCTTTTGGAAGATAATGATCATGTCTGGCTCGAGATTCGCCATACACATATAGCAGATGCTAGTGAACGATTGAGCGACAAGTTCTCCACCTTTCAATCAAAGAATAAAGCTGCACAGCTCCAACAGAGTGCAAGAGATGGCAATGAATTATCTACACGGGACTTGCAGAAAATGGTTCAAGCTCTGCCACAATACACTGAACAAATGGAAAAGATTTCACTCCATGTTGAGATTGCAGGAAAACTTAACAAAATTATCAGGGAAACGGGACTTCGAGACCTTGGGCAACTGGAGCAGGATCTTGTGTTTGGAGATGCAGGAGCCAAGGATGTCATCAATTATCTGAGGACCAATCAGGATACAAGTCCTGAAAATAAGTTGCGTTTGCTAATGATTTATGCCTCTGTATATCCTGAGAAGTTTGAAGGGGACAAAGCTAATAAGCTAATGCAGTTGGCAAGGTTATCACATGAGGACATGAAGGTTGTGGATAACATGCGATCACTTAAGGGATCATCAGATTCCAAAAAGACGACTGGTAGCTTTTCACTCAAGTTCAATAATGCGAAGACTAAACAAGCAGCGAGAAAGGATCGTGAAGGTGAAGAGGAGGAAACGTGGCAACTATTCCGATTTTTTCCACTGATGGAGGAACTCATTGAAAATCTTAACAAAGGTCTATTACCAAAGGATGAGTATTCATGTATGAATGAACCTGGTCCAACTCCTGAGCCAGGAAGCCAGAGGCGTACTCAGAGTACATCTGAAAGGTCAACTCAGTCGACTAATCCCCATTCGATAAGATCAAGGAGAACTGCAAATTGGGGACGGGCTCGGCATTCTGATGATGGATATTCAAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 map04721
Pfam Domains
Protein Families
Sec1

Protein Analysis

594

Amino Acids

68.14

Weight (kDa)

6.13

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 45 - 572 6e-116 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 129
AccI GTMKAC 1 cut(s) 1703
AciI CCGC 1 cut(s) 36
AclWI GGATC 5 cut(s) 109, 896, 1227, 1441, 1521
AcsI RAATTY 4 cut(s) 118, 210, 448, 532
AdeI CACNNNGTG 1 cut(s) 668
AfaI GTAC 3 cut(s) 50, 1672, 1681
AfiI CCNNNNNNNGG 3 cut(s) 129, 428, 1655
AflII CTTAAG 1 cut(s) 1427
AflIII ACRYGT 1 cut(s) 318
AgeI ACCGGT 1 cut(s) 78
AjnI CCWGG 2 cut(s) 1636, 1654
AjuI GAANNNNNNNTTGG 2 cut(s) 1726, 1758
AluBI AGCT 9 cut(s) 514, 607, 688, 1031, 1039, 1103, 1358, 1366, 1464
AluI AGCT 9 cut(s) 514, 607, 688, 1031, 1039, 1103, 1358, 1366, 1464
Alw21I GWGCWC 1 cut(s) 141
Alw26I GTCTC 2 cut(s) 46, 1191
AlwI GGATC 5 cut(s) 109, 896, 1227, 1441, 1521
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 950
ApeKI GCWGC 3 cut(s) 607, 1031, 1502
ApoI RAATTY 4 cut(s) 118, 210, 448, 532
AsiGI ACCGGT 1 cut(s) 78
Asp700I GAANNNNTTC 2 cut(s) 350, 459
AspS9I GGNCC 3 cut(s) 919, 1269, 1640
AsuHPI GGTGA 3 cut(s) 360, 533, 1535
AsuII TTCGAA 1 cut(s) 235
AvaI CYCGRG 1 cut(s) 950
AvaII GGWCC 3 cut(s) 919, 1269, 1640
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BanII GRGCYC 1 cut(s) 1756
BauI CACGAG 1 cut(s) 666
BbsI GAAGAC 2 cut(s) 231, 1496
Bbv12I GWGCWC 1 cut(s) 141
BbvI GCAGC 3 cut(s) 594, 1018, 1514
BccI CCATC 4 cut(s) 851, 1052, 1561, 1763
BceAI ACGGC 2 cut(s) 57, 238
BciT130I CCWGG 2 cut(s) 1638, 1656
BciVI GTATCC 1 cut(s) 1273
BclI TGATCA 3 cut(s) 490, 790, 937
BcoDI GTCTC 2 cut(s) 46, 1191
BfaI CTAG 2 cut(s) 885, 981
BfmI CTRYAG 1 cut(s) 651
BfrI CTTAAG 1 cut(s) 1427
BfuI GTATCC 1 cut(s) 1273
BisI GCNGC 3 cut(s) 608, 1032, 1503
BlsI GCNGC 3 cut(s) 609, 1033, 1504
Bme1390I CCNGG 2 cut(s) 1638, 1656
Bme18I GGWCC 3 cut(s) 919, 1269, 1640
BmeT110I CYCGRG 1 cut(s) 950
BmgT120I GGNCC 3 cut(s) 919, 1269, 1640
BmiI GGNNCC 4 cut(s) 246, 333, 882, 1243
BmrFI CCNGG 2 cut(s) 1638, 1656
BmsI GCATC 3 cut(s) 817, 967, 1225
BpiI GAAGAC 2 cut(s) 231, 1496
BplI GAGNNNNNCTC 2 cut(s) 1657, 1689
BpmI CTGGAG 1 cut(s) 1232
Bpu10I CCTNAGC 1 cut(s) 1650
Bpu14I TTCGAA 1 cut(s) 235
BpuEI CTTGAG 1 cut(s) 1457
BsaAI YACGTR 1 cut(s) 425
BsaBI GATNNNNATC 1 cut(s) 936
BsaI GGTCTC 1 cut(s) 1191
BsaJI CCNNGG 4 cut(s) 31, 633, 1201, 1245
BsaWI WCCGGW 1 cut(s) 78
BsaXI ACNNNNNCTCC 6 cut(s) 38, 68, 989, 1019, 1523, 1553
Bsc4I CCNNNNNNNGG 3 cut(s) 129, 428, 1655
Bse118I RCCGGY 1 cut(s) 78
Bse1I ACTGG 4 cut(s) 645, 898, 1215, 1462
Bse3DI GCAATG 2 cut(s) 248, 1069
Bse8I GATNNNNATC 1 cut(s) 936
BseBI CCWGG 2 cut(s) 1638, 1656
BseDI CCNNGG 4 cut(s) 31, 633, 1201, 1245
BseGI GGATG 5 cut(s) 268, 649, 862, 1255, 1618
BseJI GATNNNNATC 1 cut(s) 936
BseLI CCNNNNNNNGG 3 cut(s) 129, 428, 1655
BseMI GCAATG 2 cut(s) 248, 1069
BseMII CTCAG 6 cut(s) 897, 1256, 1326, 1641, 1688, 1712
BseNI ACTGG 4 cut(s) 645, 898, 1215, 1462
BseRI GAGGAG 2 cut(s) 524, 1544
BseXI GCAGC 3 cut(s) 594, 1018, 1514
BsgI GTGCAG 1 cut(s) 1017
BshTI ACCGGT 1 cut(s) 78
BsiHKAI GWGCWC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 950
BsiSI CCGG 1 cut(s) 79
BslFI GGGAC 6 cut(s) 157, 719, 1094, 1203, 1364, 1761
BslI CCNNNNNNNGG 3 cut(s) 129, 428, 1655
BsmAI GTCTC 2 cut(s) 46, 1191
BsmFI GGGAC 6 cut(s) 157, 719, 1094, 1203, 1364, 1761
BsmI GAATGC 1 cut(s) 1759
Bso31I GGTCTC 1 cut(s) 1191
BsoBI CYCGRG 1 cut(s) 950
Bsp119I TTCGAA 1 cut(s) 235
Bsp1286I GDGCHC 2 cut(s) 141, 1756
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 1 cut(s) 36
BspCNI CTCAG 6 cut(s) 898, 1257, 1327, 1642, 1687, 1711
BspLI GGNNCC 4 cut(s) 246, 333, 882, 1243
BspMAI CTGCAG 1 cut(s) 655
BspPI GGATC 5 cut(s) 109, 896, 1227, 1441, 1521
BspT104I TTCGAA 1 cut(s) 235
BspTI CTTAAG 1 cut(s) 1427
BspTNI GGTCTC 1 cut(s) 1191
BsrDI GCAATG 2 cut(s) 248, 1069
BsrFI RCCGGY 1 cut(s) 78
BsrI ACTGG 4 cut(s) 645, 898, 1215, 1462
BssAI RCCGGY 1 cut(s) 78
BssECI CCNNGG 4 cut(s) 31, 633, 1201, 1245
BssSI CACGAG 1 cut(s) 666
BssT1I CCWWGG 4 cut(s) 31, 633, 1201, 1245
Bst2BI CACGAG 1 cut(s) 666
Bst2UI CCWGG 2 cut(s) 1638, 1656
Bst4CI ACNGT 4 cut(s) 160, 586, 626, 787
Bst6I CTCTTC 1 cut(s) 1521
BstAFI CTTAAG 1 cut(s) 1427
BstAPI GCANNNNNTGC 1 cut(s) 94
BstBAI YACGTR 1 cut(s) 425
BstBI TTCGAA 1 cut(s) 235
BstC8I GCNNGC 1 cut(s) 690
BstDEI CTNAG 7 cut(s) 537, 906, 1265, 1335, 1650, 1674, 1698
BstDSI CCRYGG 1 cut(s) 31
BstENI CCTNNNNNAGG 1 cut(s) 1653
BstF5I GGATG 5 cut(s) 268, 649, 862, 1255, 1618
BstMAI GTCTC 2 cut(s) 46, 1191
BstMWI GCNNNNNNNGC 4 cut(s) 94, 616, 635, 1213
BstNI CCWGG 2 cut(s) 1638, 1656
BstNSI RCATGY 3 cut(s) 322, 762, 1420
BstSCI CCNGG 2 cut(s) 1636, 1654
BstSFI CTRYAG 1 cut(s) 651
BstV1I GCAGC 3 cut(s) 594, 1018, 1514
BstV2I GAAGAC 2 cut(s) 231, 1496
BstX2I RGATCY 2 cut(s) 901, 1219
BstXI CCANNNNNNTGG 1 cut(s) 640
BstYI RGATCY 2 cut(s) 901, 1219
BsuI GTATCC 1 cut(s) 1273
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 5 cut(s) 268, 649, 862, 1255, 1618
BtsIMutI CAGTG 5 cut(s) 165, 486, 638, 1116, 1562
Cac8I GCNNGC 1 cut(s) 690
CaiI CAGNNNCTG 1 cut(s) 17
Cfr10I RCCGGY 1 cut(s) 78
Cfr13I GGNCC 3 cut(s) 919, 1269, 1640
CsiI ACCWGGT 1 cut(s) 1636
Csp6I GTAC 3 cut(s) 49, 1671, 1680
CspAI ACCGGT 1 cut(s) 78
CspCI CAANNNNNGTGG 2 cut(s) 877, 912
CviQI GTAC 3 cut(s) 49, 1671, 1680
DdeI CTNAG 7 cut(s) 537, 906, 1265, 1335, 1650, 1674, 1698
DraIII CACNNNGTG 1 cut(s) 668
Eam1104I CTCTTC 1 cut(s) 1521
EarI CTCTTC 1 cut(s) 1521
EciI GGCGGA 1 cut(s) 51
Eco130I CCWWGG 4 cut(s) 31, 633, 1201, 1245
Eco24I GRGCYC 1 cut(s) 1756
Eco31I GGTCTC 1 cut(s) 1191
Eco32I GATATC 1 cut(s) 196
Eco47I GGWCC 3 cut(s) 919, 1269, 1640
Eco88I CYCGRG 1 cut(s) 950
EcoNI CCTNNNNNAGG 1 cut(s) 1653
EcoO109I RGGNCCY 1 cut(s) 919
EcoRI GAATTC 2 cut(s) 118, 532
EcoRII CCWGG 2 cut(s) 1636, 1654
EcoRV GATATC 1 cut(s) 196
EcoT14I CCWWGG 4 cut(s) 31, 633, 1201, 1245
EcoT38I GRGCYC 1 cut(s) 1756
ErhI CCWWGG 4 cut(s) 31, 633, 1201, 1245
FalI AAGNNNNNCTT 2 cut(s) 319, 351
FaqI GGGAC 6 cut(s) 157, 719, 1094, 1203, 1364, 1761
FauNDI CATATG 2 cut(s) 613, 823
FbaI TGATCA 3 cut(s) 490, 790, 937
FblI GTMKAC 1 cut(s) 1703
Fnu4HI GCNGC 3 cut(s) 608, 1032, 1503
FokI GGATG 5 cut(s) 275, 656, 869, 1262, 1625
FriOI GRGCYC 1 cut(s) 1756
Fsp4HI GCNGC 3 cut(s) 608, 1032, 1503
FspBI CTAG 2 cut(s) 885, 981
GluI GCNGC 3 cut(s) 608, 1032, 1503
GsuI CTGGAG 1 cut(s) 1232
HapII CCGG 1 cut(s) 79
HincII GTYRAC 2 cut(s) 1695, 1704
HindII GTYRAC 2 cut(s) 1695, 1704
HindIII AAGCTT 1 cut(s) 686
HinfI GANTC 7 cut(s) 14, 20, 39, 543, 647, 955, 1442
HpaII CCGG 1 cut(s) 79
HphI GGTGA 3 cut(s) 360, 533, 1535
Hpy166II GTNNAC 4 cut(s) 368, 986, 1695, 1704
Hpy8I GTNNAC 4 cut(s) 368, 986, 1695, 1704
HpyAV CCTTC 7 cut(s) 127, 231, 321, 595, 1340, 1396, 1514
HpyCH4III ACNGT 4 cut(s) 160, 586, 626, 787
HpyCH4IV ACGT 2 cut(s) 424, 1538
HpyF10VI GCNNNNNNNGC 4 cut(s) 94, 616, 635, 1213
HpyF3I CTNAG 7 cut(s) 537, 906, 1265, 1335, 1650, 1674, 1698
HpySE526I ACGT 2 cut(s) 424, 1538
Ksp22I TGATCA 3 cut(s) 490, 790, 937
LmnI GCTCC 8 cut(s) 244, 331, 511, 604, 805, 1044, 1213, 1241
Lsp1109I GCAGC 3 cut(s) 594, 1018, 1514
LweI GCATC 3 cut(s) 817, 967, 1225
MabI ACCWGGT 1 cut(s) 1636
MaeI CTAG 2 cut(s) 885, 981
MaeII ACGT 2 cut(s) 424, 1538
MaeIII GTNAC 3 cut(s) 74, 154, 836
MboII GAAGA 6 cut(s) 18, 236, 720, 941, 1501, 1538
MflI RGATCY 2 cut(s) 901, 1219
MhlI GDGCHC 2 cut(s) 141, 1756
MlyI GAGTC 3 cut(s) 8, 48, 552
MmeI TCCRAC 2 cut(s) 1066, 1667
MroXI GAANNNNTTC 2 cut(s) 350, 459
MseI TTAA 4 cut(s) 674, 1167, 1428, 1590
MslI CAYNNNNRTG 1 cut(s) 1764
MspCI CTTAAG 1 cut(s) 1427
MspI CCGG 1 cut(s) 79
MspR9I CCNGG 2 cut(s) 1638, 1656
Mva1269I GAATGC 1 cut(s) 1759
MvaI CCWGG 2 cut(s) 1638, 1656
MwoI GCNNNNNNNGC 4 cut(s) 94, 616, 635, 1213
NcoI CCATGG 1 cut(s) 31
NdeI CATATG 2 cut(s) 613, 823
NlaIV GGNNCC 4 cut(s) 246, 333, 882, 1243
NmeAIII GCCGAG 1 cut(s) 1735
NmuCI GTSAC 3 cut(s) 74, 154, 836
NspI RCATGY 3 cut(s) 322, 762, 1420
NspV TTCGAA 1 cut(s) 235
PaeR7I CTCGAG 1 cut(s) 950
PciI ACATGT 1 cut(s) 318
PctI GAATGC 1 cut(s) 1759
PdmI GAANNNNTTC 2 cut(s) 350, 459
PfeI GAWTC 4 cut(s) 20, 647, 955, 1442
PflMI CCANNNNNTGG 1 cut(s) 129
PinAI ACCGGT 1 cut(s) 78
PkrI GCNGC 3 cut(s) 609, 1033, 1504
PleI GAGTC 3 cut(s) 8, 47, 551
PpsI GAGTC 3 cut(s) 8, 47, 551
Ppu21I YACGTR 1 cut(s) 425
PpuMI RGGWCCY 1 cut(s) 919
PscI ACATGT 1 cut(s) 318
Psp5II RGGWCCY 1 cut(s) 919
Psp6I CCWGG 2 cut(s) 1636, 1654
PspGI CCWGG 2 cut(s) 1636, 1654
PspN4I GGNNCC 4 cut(s) 246, 333, 882, 1243
PspPI GGNCC 3 cut(s) 919, 1269, 1640
PspPPI RGGWCCY 1 cut(s) 919
PstI CTGCAG 1 cut(s) 655
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 2 cut(s) 901, 1219
RsaI GTAC 3 cut(s) 50, 1672, 1681
RsaNI GTAC 3 cut(s) 49, 1671, 1680
RseI CAYNNNNRTG 1 cut(s) 1764
SalI GTCGAC 1 cut(s) 1702
SaqAI TTAA 4 cut(s) 674, 1167, 1428, 1590
SatI GCNGC 3 cut(s) 608, 1032, 1503
Sau96I GGNCC 3 cut(s) 919, 1269, 1640
SchI GAGTC 3 cut(s) 8, 48, 552
ScrFI CCNGG 2 cut(s) 1638, 1656
SduI GDGCHC 2 cut(s) 141, 1756
SexAI ACCWGGT 1 cut(s) 1636
SfaNI GCATC 3 cut(s) 817, 967, 1225
SfcI CTRYAG 1 cut(s) 651
Sfr274I CTCGAG 1 cut(s) 950
SfuI TTCGAA 1 cut(s) 235
SinI GGWCC 3 cut(s) 919, 1269, 1640
SlaI CTCGAG 1 cut(s) 950
SmiMI CAYNNNNRTG 1 cut(s) 1764
SmlI CTYRAG 3 cut(s) 950, 1427, 1472
SmoI CTYRAG 3 cut(s) 950, 1427, 1472
SsiI CCGC 1 cut(s) 36
SspI AATATT 1 cut(s) 298
SspMI CTAG 2 cut(s) 885, 981
StyD4I CCNGG 2 cut(s) 1636, 1654
StyI CCWWGG 4 cut(s) 31, 633, 1201, 1245
TaaI ACNGT 4 cut(s) 160, 586, 626, 787
TaiI ACGT 2 cut(s) 427, 1541
TaqI TCGA 7 cut(s) 235, 546, 574, 951, 1195, 1703, 1718
TatI WGTACW 2 cut(s) 48, 1679
TfiI GAWTC 4 cut(s) 20, 647, 955, 1442
Tru1I TTAA 4 cut(s) 674, 1167, 1428, 1590
Tru9I TTAA 4 cut(s) 674, 1167, 1428, 1590
TscAI CASTG 5 cut(s) 165, 493, 645, 1123, 1569
TseFI GTSAC 3 cut(s) 74, 154, 836
TseI GCWGC 3 cut(s) 607, 1031, 1502
Tsp45I GTSAC 3 cut(s) 74, 154, 836
TspGWI ACGGA 2 cut(s) 50, 128
TspRI CASTG 5 cut(s) 165, 493, 645, 1123, 1569
Van91I CCANNNNNTGG 1 cut(s) 129
Vha464I CTTAAG 1 cut(s) 1427
VpaK11BI GGWCC 3 cut(s) 919, 1269, 1640
XagI CCTNNNNNAGG 1 cut(s) 1653
XapI RAATTY 4 cut(s) 118, 210, 448, 532
XceI RCATGY 3 cut(s) 322, 762, 1420
XhoI CTCGAG 1 cut(s) 950
XmiI GTMKAC 1 cut(s) 1703
XmnI GAANNNNTTC 2 cut(s) 350, 459
XspI CTAG 2 cut(s) 885, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.