Rh1DG222600

Belongs to the STXBP unc-18 SEC1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005583
Physical Location & Seq
Forward (+)
43275296 .. 43283653
8358 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG222600.1

Sequence Viewer

Length: 2037 bp
ATGTCGTTTTCAGACTCTGAATCCTCTTCCCATGGCGGAGTCTCGGAGTACAAGTGTTTCCGTCAGTTCAGCCGTGACCATTCTTTGGATGGTTTTGTAGGGTTATTGCACGAAATGCTTGGATCAACCAGAACGGAGAATTCCAAGTCTTGGAAGGTGCTCATCATGGATAAAGTGACAGTGAAAGTCATGTCCCATTCATGTAAAATGGCAGATATCACAGACCAAGAAATTTCATTGGTGGAAGACCTTTTTCGGAGGAGGGAGCCATTGCCGTCAATGGATGTTATTTATTTTATCCAGCCAACAAAAGAGAATATTGTCATGCTCTTGTCTGACATGTCTGGAAGGGAGCCTTTATACAGGAAAGCGTTCATATTTTTCAGTTCACCTATCCCAAAGGAACTTGTAAATCACATAAAGAGTGATACAAGTGTTTTACCACGTATAGGTGCATTGAGAGAGATGAATTTGGAATACTTTCCCATAGATAATCAGGCTTTTATCACTGATCAAGATAGGGCATTGGAGGAGCTTTTCGGTGATGTTGAGAATTCTAAGAGAGTCGAGAAGTGCTTGAACATAATGGCAACTCGAATTGCGACAGTTTTTGCTTCAATGAAGGAGCTGCCATATGTGCGATACCGTGCTTCCAAGGCACTGGATGAATCTGCAGAAGCAACACCTCGTGATTTAATTCCTACAAAGCTTGCTACTGCTGTTTGGGACATTCTTTCAAAATATAAATCTTCTATTCCCAACTTTCCACAGAAAGAAACATGTGACTTACTCATCTTGGATAGAACTGTTGATCAGATTGCTCCTGTCATACACGAATGGACATATGATGCTATGTGTCACGATTTATTGAATATGGATGGCAACAAATATGTGCATGAGGTTCCTAGCAAAACTGGTGGAGATCCTGAGAGAAAGGAGGTCCTTTTGGAAGATAATGATCCTGTCTGGCTCGAGATTCGCCATACACATATAGCAGATGCTAGTGAACGATTGAGCGACAAGTTCTCCACCTTTCAATCAAAGAATAAAGCTGCACAGCTCCAACAGAGTGCAAGAGATGGCAATGAATTATCTACACGGGACTTGCAGAAAATGGTTCAAGCTCTGCCACAATACACTGAACAAATGGAAAAGATTTCTCTCCATGTTGAGATTGCAGGAAAACTTAACAAAATCATCAGGGAAACAGGACTTCGAGACCTTGGGCAACTGGAGCAGGATCTTGTTTTTGGAGATGCAGGAGCCAAGGATGTCATCAATTATCTGAGGACCAATCAGGATACAAGTCCTGAAAATAAGTTGCGTTTGCTAATGATTTATGCCTCTGTATATCCTGAGAAGTTTGAAGGGGACAAAGCTAATAAGCTAATGCAGTTGGCAAGGTTATCACATGAGGACATGAAGGTTGTGGATAACATGCGATCGCTTAAGGGATCATCAGATTCCAAAAAGACGACTGGTAGCTTTTCACTCAAGTTCAATAATGCGAAGACTAAACAAGCAGCGAGAAAGGATCGTGAAGGTGAAGAGGAGGAAACGTGGCAACTATTCCGATTTTTTCCACTGATGGAGGAACTCATTGAAAATCTTAACAAAGGTCTATTACCAAAGGATGAGTATTCATGTATGAATGAACCTGGTCCAACTCCTGAGCCAGGAAGCCAGAGGCGTACTCAGAGTACATCTGAAAGGTCAACTCAGTCGACTAATCCCCATTCGATAAGATCAAGGAGAACTGCAAATTGGGGACGGGCTCGGCATTCTGATGATGGATATTCAAGTGATTCGATTTTGAGAGGTGAGTCTACGGATTTCAAGAAGTTGGGGAAACGTATTTTTGTATTTATGATTGGTGGGGCAACTCGATCGGAGCTACGAGTTTGTTACAAGATGACAACAAAATTGAAAAGGGAAGTAATCCTTGGAACTACCAGTATCCAAGAACCACCACAGTATATTACGAAACTGAAGCTGCTATCAGAAAAAGAACTGTCAGTTGATGGCCTTAAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04721 map04721
Pfam Domains
Protein Families
Sec1

Protein Analysis

678

Amino Acids

77.38

Weight (kDa)

6.45

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec1 PF00995 52 - 661 3.6e-132 Sec1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 85, 150
AccI GTMKAC 2 cut(s) 1724, 1826
AciI CCGC 1 cut(s) 36
AclWI GGATC 6 cut(s) 130, 917, 953, 1248, 1462, 1542
AcsI RAATTY 4 cut(s) 139, 231, 469, 553
AcuI CTGAAG 1 cut(s) 2009
AdeI CACNNNGTG 1 cut(s) 689
AfaI GTAC 3 cut(s) 50, 1693, 1702
AfiI CCNNNNNNNGG 4 cut(s) 85, 150, 449, 1676
AflII CTTAAG 1 cut(s) 1448
AflIII ACRYGT 2 cut(s) 339, 779
AjnI CCWGG 2 cut(s) 1657, 1675
AjuI GAANNNNNNNTTGG 4 cut(s) 1747, 1779, 1926, 1958
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 2 cut(s) 46, 1212
AlwI GGATC 6 cut(s) 130, 917, 953, 1248, 1462, 1542
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 971
AoxI GGCC 1 cut(s) 2023
ApeKI GCWGC 4 cut(s) 628, 1052, 1523, 1993
ApoI RAATTY 4 cut(s) 139, 231, 469, 553
AsiSI GCGATCGC 1 cut(s) 1445
Asp700I GAANNNNTTC 2 cut(s) 371, 480
AspS9I GGNCC 3 cut(s) 940, 1290, 1661
AsuHPI GGTGA 4 cut(s) 381, 554, 1556, 1832
AvaI CYCGRG 1 cut(s) 971
AvaII GGWCC 3 cut(s) 940, 1290, 1661
BaeI ACNNNNGTAYC 2 cut(s) 420, 453
BanII GRGCYC 1 cut(s) 1777
BauI CACGAG 1 cut(s) 687
BbsI GAAGAC 2 cut(s) 252, 1517
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 4 cut(s) 615, 1039, 1535, 1980
BccI CCATC 6 cut(s) 83, 872, 1073, 1582, 1784, 2015
BceAI ACGGC 2 cut(s) 57, 259
BcgI CGANNNNNNTGC 2 cut(s) 1869, 1903
BciT130I CCWGG 2 cut(s) 1659, 1677
BciVI GTATCC 2 cut(s) 1294, 1967
BclI TGATCA 2 cut(s) 511, 811
BcoDI GTCTC 2 cut(s) 46, 1212
BfaI CTAG 2 cut(s) 906, 1002
BfmI CTRYAG 1 cut(s) 672
BfrI CTTAAG 1 cut(s) 1448
BfuI GTATCC 2 cut(s) 1294, 1967
BisI GCNGC 4 cut(s) 629, 1053, 1524, 1994
BlsI GCNGC 4 cut(s) 630, 1054, 1525, 1995
Bme1390I CCNGG 2 cut(s) 1659, 1677
Bme18I GGWCC 3 cut(s) 940, 1290, 1661
BmeT110I CYCGRG 1 cut(s) 971
BmgT120I GGNCC 3 cut(s) 940, 1290, 1661
BmiI GGNNCC 4 cut(s) 267, 354, 903, 1264
BmrFI CCNGG 2 cut(s) 1659, 1677
BmsI GCATC 3 cut(s) 838, 988, 1246
BpiI GAAGAC 2 cut(s) 252, 1517
BplI GAGNNNNNCTC 2 cut(s) 1678, 1710
BpmI CTGGAG 1 cut(s) 1253
Bpu10I CCTNAGC 1 cut(s) 1671
BpuEI CTTGAG 1 cut(s) 1478
BsaAI YACGTR 1 cut(s) 446
BsaBI GATNNNNATC 1 cut(s) 957
BsaI GGTCTC 1 cut(s) 1212
BsaJI CCNNGG 5 cut(s) 31, 654, 1222, 1266, 1942
BsaXI ACNNNNNCTCC 6 cut(s) 38, 68, 1010, 1040, 1544, 1574
Bsc4I CCNNNNNNNGG 4 cut(s) 85, 150, 449, 1676
Bse1I ACTGG 5 cut(s) 666, 919, 1236, 1483, 1953
Bse3DI GCAATG 2 cut(s) 269, 1090
Bse8I GATNNNNATC 1 cut(s) 957
BseBI CCWGG 2 cut(s) 1659, 1677
BseDI CCNNGG 5 cut(s) 31, 654, 1222, 1266, 1942
BseGI GGATG 6 cut(s) 94, 289, 670, 883, 1276, 1639
BseJI GATNNNNATC 1 cut(s) 957
BseLI CCNNNNNNNGG 4 cut(s) 85, 150, 449, 1676
BseMI GCAATG 2 cut(s) 269, 1090
BseMII CTCAG 6 cut(s) 918, 1277, 1347, 1662, 1709, 1733
BseNI ACTGG 5 cut(s) 666, 919, 1236, 1483, 1953
BseRI GAGGAG 3 cut(s) 274, 545, 1565
BseXI GCAGC 4 cut(s) 615, 1039, 1535, 1980
BsgI GTGCAG 1 cut(s) 1038
Bsh1285I CGRYCG 2 cut(s) 1445, 1889
BshFI GGCC 1 cut(s) 2025
BsiEI CGRYCG 2 cut(s) 1445, 1889
BsiHKAI GWGCWC 1 cut(s) 162
BsiHKCI CYCGRG 1 cut(s) 971
BslFI GGGAC 5 cut(s) 178, 740, 1115, 1385, 1782
BslI CCNNNNNNNGG 4 cut(s) 85, 150, 449, 1676
BsmAI GTCTC 2 cut(s) 46, 1212
BsmFI GGGAC 5 cut(s) 178, 740, 1115, 1385, 1782
BsmI GAATGC 1 cut(s) 1780
BsnI GGCC 1 cut(s) 2025
Bso31I GGTCTC 1 cut(s) 1212
BsoBI CYCGRG 1 cut(s) 971
Bsp1286I GDGCHC 2 cut(s) 162, 1777
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 1 cut(s) 36
BspANI GGCC 1 cut(s) 2025
BspCNI CTCAG 6 cut(s) 919, 1278, 1348, 1663, 1708, 1732
BspLI GGNNCC 4 cut(s) 267, 354, 903, 1264
BspMAI CTGCAG 1 cut(s) 676
BspPI GGATC 6 cut(s) 130, 917, 953, 1248, 1462, 1542
BspTI CTTAAG 1 cut(s) 1448
BspTNI GGTCTC 1 cut(s) 1212
BsrDI GCAATG 2 cut(s) 269, 1090
BsrI ACTGG 5 cut(s) 666, 919, 1236, 1483, 1953
BssECI CCNNGG 5 cut(s) 31, 654, 1222, 1266, 1942
BssSI CACGAG 1 cut(s) 687
BssT1I CCWWGG 5 cut(s) 31, 654, 1222, 1266, 1942
Bst2BI CACGAG 1 cut(s) 687
Bst2UI CCWGG 2 cut(s) 1659, 1677
Bst4CI ACNGT 6 cut(s) 181, 607, 647, 808, 1974, 2013
Bst6I CTCTTC 2 cut(s) 31, 1542
BstAFI CTTAAG 1 cut(s) 1448
BstAPI GCANNNNNTGC 1 cut(s) 115
BstBAI YACGTR 1 cut(s) 446
BstC8I GCNNGC 1 cut(s) 711
BstDEI CTNAG 7 cut(s) 558, 927, 1286, 1356, 1671, 1695, 1719
BstDSI CCRYGG 1 cut(s) 31
BstENI CCTNNNNNAGG 1 cut(s) 1674
BstF5I GGATG 6 cut(s) 94, 289, 670, 883, 1276, 1639
BstMAI GTCTC 2 cut(s) 46, 1212
BstMCI CGRYCG 2 cut(s) 1445, 1889
BstMWI GCNNNNNNNGC 4 cut(s) 115, 637, 656, 1234
BstNI CCWGG 2 cut(s) 1659, 1677
BstNSI RCATGY 3 cut(s) 343, 783, 1441
BstSCI CCNGG 2 cut(s) 1657, 1675
BstSFI CTRYAG 1 cut(s) 672
BstV1I GCAGC 4 cut(s) 615, 1039, 1535, 1980
BstV2I GAAGAC 2 cut(s) 252, 1517
BstX2I RGATCY 2 cut(s) 922, 1240
BstXI CCANNNNNNTGG 1 cut(s) 661
BstYI RGATCY 2 cut(s) 922, 1240
BsuI GTATCC 2 cut(s) 1294, 1967
BsuRI GGCC 1 cut(s) 2025
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 6 cut(s) 94, 289, 670, 883, 1276, 1639
BtsIMutI CAGTG 5 cut(s) 186, 507, 659, 1137, 1583
Cac8I GCNNGC 1 cut(s) 711
CaiI CAGNNNCTG 1 cut(s) 17
Cfr13I GGNCC 3 cut(s) 940, 1290, 1661
CsiI ACCWGGT 1 cut(s) 1657
Csp6I GTAC 3 cut(s) 49, 1692, 1701
CspCI CAANNNNNGTGG 2 cut(s) 898, 933
CviQI GTAC 3 cut(s) 49, 1692, 1701
DdeI CTNAG 7 cut(s) 558, 927, 1286, 1356, 1671, 1695, 1719
DraIII CACNNNGTG 1 cut(s) 689
Eam1104I CTCTTC 2 cut(s) 31, 1542
EarI CTCTTC 2 cut(s) 31, 1542
EciI GGCGGA 1 cut(s) 51
Eco130I CCWWGG 5 cut(s) 31, 654, 1222, 1266, 1942
Eco24I GRGCYC 1 cut(s) 1777
Eco31I GGTCTC 1 cut(s) 1212
Eco32I GATATC 1 cut(s) 217
Eco47I GGWCC 3 cut(s) 940, 1290, 1661
Eco57I CTGAAG 1 cut(s) 2009
Eco88I CYCGRG 1 cut(s) 971
EcoNI CCTNNNNNAGG 1 cut(s) 1674
EcoO109I RGGNCCY 1 cut(s) 940
EcoRI GAATTC 2 cut(s) 139, 553
EcoRII CCWGG 2 cut(s) 1657, 1675
EcoRV GATATC 1 cut(s) 217
EcoT14I CCWWGG 5 cut(s) 31, 654, 1222, 1266, 1942
EcoT38I GRGCYC 1 cut(s) 1777
ErhI CCWWGG 5 cut(s) 31, 654, 1222, 1266, 1942
FalI AAGNNNNNCTT 4 cut(s) 340, 372, 1926, 1958
FaqI GGGAC 5 cut(s) 178, 740, 1115, 1385, 1782
FauNDI CATATG 2 cut(s) 634, 844
FbaI TGATCA 2 cut(s) 511, 811
FblI GTMKAC 2 cut(s) 1724, 1826
Fnu4HI GCNGC 4 cut(s) 629, 1053, 1524, 1994
FokI GGATG 6 cut(s) 101, 296, 677, 890, 1283, 1646
FriOI GRGCYC 1 cut(s) 1777
Fsp4HI GCNGC 4 cut(s) 629, 1053, 1524, 1994
FspBI CTAG 2 cut(s) 906, 1002
GluI GCNGC 4 cut(s) 629, 1053, 1524, 1994
GsuI CTGGAG 1 cut(s) 1253
HaeIII GGCC 1 cut(s) 2025
HincII GTYRAC 2 cut(s) 1716, 1725
HindII GTYRAC 2 cut(s) 1716, 1725
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 9 cut(s) 14, 20, 39, 564, 668, 976, 1463, 1805, 1823
HphI GGTGA 4 cut(s) 381, 554, 1556, 1832
Hpy166II GTNNAC 5 cut(s) 389, 1007, 1716, 1725, 1827
Hpy8I GTNNAC 5 cut(s) 389, 1007, 1716, 1725, 1827
HpyAV CCTTC 6 cut(s) 148, 342, 616, 1361, 1417, 1535
HpyCH4III ACNGT 6 cut(s) 181, 607, 647, 808, 1974, 2013
HpyCH4IV ACGT 3 cut(s) 445, 1559, 1852
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 637, 656, 1234
HpyF3I CTNAG 7 cut(s) 558, 927, 1286, 1356, 1671, 1695, 1719
HpySE526I ACGT 3 cut(s) 445, 1559, 1852
Ksp22I TGATCA 2 cut(s) 511, 811
LmnI GCTCC 9 cut(s) 265, 352, 532, 625, 826, 1065, 1234, 1262, 1891
Lsp1109I GCAGC 4 cut(s) 615, 1039, 1535, 1980
LweI GCATC 3 cut(s) 838, 988, 1246
MabI ACCWGGT 1 cut(s) 1657
MaeI CTAG 2 cut(s) 906, 1002
MaeII ACGT 3 cut(s) 445, 1559, 1852
MaeIII GTNAC 5 cut(s) 74, 175, 782, 857, 1904
MboII GAAGA 6 cut(s) 18, 257, 741, 962, 1522, 1559
MflI RGATCY 2 cut(s) 922, 1240
MhlI GDGCHC 2 cut(s) 162, 1777
MlyI GAGTC 4 cut(s) 8, 48, 573, 1832
MmeI TCCRAC 2 cut(s) 1087, 1688
MroXI GAANNNNTTC 2 cut(s) 371, 480
MseI TTAA 5 cut(s) 695, 1188, 1449, 1611, 2028
MslI CAYNNNNRTG 1 cut(s) 1785
MspCI CTTAAG 1 cut(s) 1448
MspR9I CCNGG 2 cut(s) 1659, 1677
Mva1269I GAATGC 1 cut(s) 1780
MvaI CCWGG 2 cut(s) 1659, 1677
MwoI GCNNNNNNNGC 4 cut(s) 115, 637, 656, 1234
NcoI CCATGG 1 cut(s) 31
NdeI CATATG 2 cut(s) 634, 844
NlaIV GGNNCC 4 cut(s) 267, 354, 903, 1264
NmeAIII GCCGAG 1 cut(s) 1756
NmuCI GTSAC 4 cut(s) 74, 175, 782, 857
NspI RCATGY 3 cut(s) 343, 783, 1441
PaeR7I CTCGAG 1 cut(s) 971
PciI ACATGT 2 cut(s) 339, 779
PctI GAATGC 1 cut(s) 1780
PdmI GAANNNNTTC 2 cut(s) 371, 480
PfeI GAWTC 5 cut(s) 20, 668, 976, 1463, 1805
PflMI CCANNNNNTGG 2 cut(s) 85, 150
PkrI GCNGC 4 cut(s) 630, 1054, 1525, 1995
Ple19I CGATCG 2 cut(s) 1445, 1889
PleI GAGTC 4 cut(s) 8, 47, 572, 1831
PpsI GAGTC 4 cut(s) 8, 47, 572, 1831
Ppu21I YACGTR 1 cut(s) 446
PpuMI RGGWCCY 1 cut(s) 940
PscI ACATGT 2 cut(s) 339, 779
Psp5II RGGWCCY 1 cut(s) 940
Psp6I CCWGG 2 cut(s) 1657, 1675
PspGI CCWGG 2 cut(s) 1657, 1675
PspN4I GGNNCC 4 cut(s) 267, 354, 903, 1264
PspPI GGNCC 3 cut(s) 940, 1290, 1661
PspPPI RGGWCCY 1 cut(s) 940
PstI CTGCAG 1 cut(s) 676
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 2 cut(s) 922, 1240
PvuI CGATCG 2 cut(s) 1445, 1889
RgaI GCGATCGC 1 cut(s) 1445
RsaI GTAC 3 cut(s) 50, 1693, 1702
RsaNI GTAC 3 cut(s) 49, 1692, 1701
RseI CAYNNNNRTG 1 cut(s) 1785
SalI GTCGAC 1 cut(s) 1723
SaqAI TTAA 5 cut(s) 695, 1188, 1449, 1611, 2028
SatI GCNGC 4 cut(s) 629, 1053, 1524, 1994
Sau96I GGNCC 3 cut(s) 940, 1290, 1661
SchI GAGTC 4 cut(s) 8, 48, 573, 1832
ScrFI CCNGG 2 cut(s) 1659, 1677
SduI GDGCHC 2 cut(s) 162, 1777
SexAI ACCWGGT 1 cut(s) 1657
SfaAI GCGATCGC 1 cut(s) 1445
SfaNI GCATC 3 cut(s) 838, 988, 1246
SfcI CTRYAG 1 cut(s) 672
Sfr274I CTCGAG 1 cut(s) 971
SgfI GCGATCGC 1 cut(s) 1445
SinI GGWCC 3 cut(s) 940, 1290, 1661
SlaI CTCGAG 1 cut(s) 971
SmiMI CAYNNNNRTG 1 cut(s) 1785
SmlI CTYRAG 3 cut(s) 971, 1448, 1493
SmoI CTYRAG 3 cut(s) 971, 1448, 1493
SsiI CCGC 1 cut(s) 36
SspI AATATT 1 cut(s) 319
SspMI CTAG 2 cut(s) 906, 1002
StyD4I CCNGG 2 cut(s) 1657, 1675
StyI CCWWGG 5 cut(s) 31, 654, 1222, 1266, 1942
TaaI ACNGT 6 cut(s) 181, 607, 647, 808, 1974, 2013
TaiI ACGT 3 cut(s) 448, 1562, 1855
TaqI TCGA 8 cut(s) 567, 595, 972, 1216, 1724, 1739, 1808, 1885
TatI WGTACW 2 cut(s) 48, 1700
TfiI GAWTC 5 cut(s) 20, 668, 976, 1463, 1805
Tru1I TTAA 5 cut(s) 695, 1188, 1449, 1611, 2028
Tru9I TTAA 5 cut(s) 695, 1188, 1449, 1611, 2028
TscAI CASTG 5 cut(s) 186, 514, 666, 1144, 1590
TseFI GTSAC 4 cut(s) 74, 175, 782, 857
TseI GCWGC 4 cut(s) 628, 1052, 1523, 1993
Tsp45I GTSAC 4 cut(s) 74, 175, 782, 857
TspGWI ACGGA 3 cut(s) 50, 149, 1844
TspRI CASTG 5 cut(s) 186, 514, 666, 1144, 1590
Van91I CCANNNNNTGG 2 cut(s) 85, 150
Vha464I CTTAAG 1 cut(s) 1448
VpaK11BI GGWCC 3 cut(s) 940, 1290, 1661
XagI CCTNNNNNAGG 1 cut(s) 1674
XapI RAATTY 4 cut(s) 139, 231, 469, 553
XceI RCATGY 3 cut(s) 343, 783, 1441
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
XhoI CTCGAG 1 cut(s) 971
XmiI GTMKAC 2 cut(s) 1724, 1826
XmnI GAANNNNTTC 2 cut(s) 371, 480
XspI CTAG 2 cut(s) 906, 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.