FvH4_7g12520

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015105
Physical Location & Seq
Reverse (-)
11328300 .. 11329265
966 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12520.t1

Sequence Viewer

Length: 966 bp
ATGTCAAAATTTGATCCCTACACCCACCTCAACATTGTCCACAACCCGATCGACGACACCTTGACCCGCCTCGTCAGCCTCCCCACCACCGACGCCAACCCCAACTCGTCTCCCGGCGATCCTGTCGCCTCCAAAGACCTAACCCTCAACTCGGAAACCAAGACGTGGCTACGAATCTTCCGACCCTCCAAGCTCCCCTCTAACGACAACACCGTCGCCCGCCTTCCTATCATCATCTACTTCCACCACGGCGGCTGGCTCCTCCTCAGCGCCGCCGACACCTCCATCCACACGAACTGCTCCCTCATCACCAGCCAAGTTCCCGCCATTCTAGTCTCGGTAAATTACCGTCTCGCCCCCGAGGCCCGGCTTCCGGCGCAGTACGAGGACGCATTGGACGCTATCCACTGGGTTAAGAAGCAAGGCGTCAACCCCGAGGGCGAGCAATGGATCCGAGACTACGGCGACCTGTCTAGGTGCTACTTGTACGGCTGCGGCTGCGGGGGCAATATCGTCTTTTACTCGGCGCTCAAAGCCTACGAGACTATGAAGTTGGAACCCATGCAGATATCTGGGATCATAATGAACCAACCCATGTTCAGCGGGATTCAGAGGACCAAGTCGGAGCTCAAATTTGCTACGGATCAGCTCTTGCCTTTGCCTGTGCTCGACCTCATGTGGGACTTAGCCTTGCCGAAATCGACGGACCGGAACCACCGTTATTGTAGTCCTGCGCTCGACGCTGGCCGGCATAAAGAGCTGATCCAGCGGCTGGGGCGGTGTTTGGTGATCGGGTTCGGAGGGGATCCGATGGTGGACAGGCAACATGAGTTTGTGACGATGTTGGTGTCTCATGGGGTACGTGTAGACGCACAGTTTGATGATCTAGGGTTCCATAATATTGATTTTGTAGACGAGAAAAGGGCTGCTGCAGTTTTGGACATTGTTAAGGAGTTTATCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

322

Amino Acids

36.02

Weight (kDa)

5.99

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 70 - 142 5.7e-09 BD-FAE
Abhydrolase_3 PF07859 78 - 299 5.3e-43 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015105)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45610
fragaria_vesca FvH4_7g12520
malus_domestica MD02G1198300.v1.1
prunus_persica Prupe.2G149700_v2.0.a1
pyrus_communis pycom02g16190
rosa_chinensis RchiOBHm_Chr1g0351421
rosa_laevigata RLG00000028408
rosa_multiflora Rmu_sc0000664.1_g000007 Rmu_sc0005320.1_g000004
rosa_roxburghii Rroxscaffold_4G00303840
rosa_rugosa Rorug01G0218000
rosa_samantha Rh1AG232500 Rh1BG203300 Rh1CG217200 Rh1DG230300
rosa_wichuraiana Rw1G020250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AccB7I CCANNNNNTGG 2 cut(s) 165, 772
AccI GTMKAC 2 cut(s) 867, 912
AclWI GGATC 9 cut(s) 8, 113, 445, 458, 584, 651, 757, 800, 813
AcoI YGGCCR 1 cut(s) 745
AcsI RAATTY 2 cut(s) 8, 632
AcyI GRCGYC 2 cut(s) 93, 426
AfaI GTAC 3 cut(s) 383, 488, 861
AfiI CCNNNNNNNGG 6 cut(s) 151, 165, 366, 373, 679, 772
AflIII ACRYGT 1 cut(s) 862
AjiI CACGTC 1 cut(s) 165
AluBI AGCT 4 cut(s) 193, 628, 649, 760
AluI AGCT 4 cut(s) 193, 628, 649, 760
Alw21I GWGCWC 2 cut(s) 630, 669
Alw26I GTCTC 6 cut(s) 114, 340, 356, 450, 536, 855
AlwI GGATC 9 cut(s) 8, 113, 445, 458, 584, 651, 757, 800, 813
AlwNI CAGNNNCTG 1 cut(s) 772
Ama87I CYCGRG 2 cut(s) 359, 434
AoxI GGCC 2 cut(s) 363, 745
ApeKI GCWGC 4 cut(s) 492, 498, 926, 929
ApoI RAATTY 2 cut(s) 8, 632
ArsI GACNNNNNNTTYG 2 cut(s) 860, 892
AspLEI GCGC 4 cut(s) 272, 379, 529, 736
AspS9I GGNCC 3 cut(s) 364, 615, 706
AsuC2I CCSGG 2 cut(s) 114, 367
AsuHPI GGTGA 2 cut(s) 301, 799
AvaI CYCGRG 2 cut(s) 359, 434
AvaII GGWCC 2 cut(s) 615, 706
BamHI GGATCC 2 cut(s) 450, 805
BanII GRGCYC 1 cut(s) 630
Bbv12I GWGCWC 2 cut(s) 630, 669
BbvCI CCTCAGC 1 cut(s) 266
BbvI GCAGC 4 cut(s) 479, 485, 913, 916
BccI CCATC 2 cut(s) 293, 805
BceAI ACGGC 3 cut(s) 265, 478, 505
BcnI CCSGG 2 cut(s) 114, 367
BcoDI GTCTC 6 cut(s) 114, 340, 356, 450, 536, 855
BfaI CTAG 3 cut(s) 332, 474, 887
BfmI CTRYAG 1 cut(s) 930
BfoI RGCGCY 2 cut(s) 273, 530
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 8 cut(s) 253, 273, 493, 496, 499, 770, 927, 930
BlsI GCNGC 8 cut(s) 254, 274, 494, 497, 500, 771, 928, 931
Bme1390I CCNGG 2 cut(s) 114, 367
Bme18I GGWCC 2 cut(s) 615, 706
BmeT110I CYCGRG 2 cut(s) 359, 434
BmgBI CACGTC 1 cut(s) 165
BmgT120I GGNCC 3 cut(s) 364, 615, 706
BmiI GGNNCC 6 cut(s) 260, 452, 558, 713, 807, 893
BmrFI CCNGG 2 cut(s) 114, 367
BmrI ACTGGG 1 cut(s) 418
BmuI ACTGGG 1 cut(s) 418
Bpu10I CCTNAGC 1 cut(s) 266
BpuMI CCSGG 2 cut(s) 114, 367
BsaAI YACGTR 1 cut(s) 863
BsaHI GRCGYC 2 cut(s) 93, 426
BsaJI CCNNGG 3 cut(s) 247, 360, 435
BsaWI WCCGGW 1 cut(s) 708
Bsc4I CCNNNNNNNGG 6 cut(s) 151, 165, 366, 373, 679, 772
Bse118I RCCGGY 1 cut(s) 747
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 452
BseDI CCNNGG 3 cut(s) 247, 360, 435
BseGI GGATG 1 cut(s) 285
BseLI CCNNNNNNNGG 6 cut(s) 151, 165, 366, 373, 679, 772
BseMI GCAATG 1 cut(s) 452
BseMII CTCAG 1 cut(s) 280
BseNI ACTGG 1 cut(s) 413
BseRI GAGGAG 2 cut(s) 251, 254
BseXI GCAGC 4 cut(s) 479, 485, 913, 916
BseYI CCCAGC 1 cut(s) 772
Bsh1285I CGRYCG 1 cut(s) 51
BshFI GGCC 2 cut(s) 365, 747
BsiEI CGRYCG 1 cut(s) 51
BsiHKAI GWGCWC 2 cut(s) 630, 669
BsiHKCI CYCGRG 2 cut(s) 359, 434
BsiSI CCGG 5 cut(s) 114, 367, 374, 709, 748
BslFI GGGAC 1 cut(s) 695
BslI CCNNNNNNNGG 6 cut(s) 151, 165, 366, 373, 679, 772
BsmAI GTCTC 6 cut(s) 114, 340, 356, 450, 536, 855
BsmBI CGTCTC 2 cut(s) 114, 356
BsmFI GGGAC 1 cut(s) 695
BsnI GGCC 2 cut(s) 365, 747
BsoBI CYCGRG 2 cut(s) 359, 434
Bsp1286I GDGCHC 2 cut(s) 630, 669
BspANI GGCC 2 cut(s) 365, 747
BspCNI CTCAG 1 cut(s) 279
BspLI GGNNCC 6 cut(s) 260, 452, 558, 713, 807, 893
BspMAI CTGCAG 1 cut(s) 934
BspPI GGATC 9 cut(s) 8, 113, 445, 458, 584, 651, 757, 800, 813
BsrDI GCAATG 1 cut(s) 452
BsrFI RCCGGY 1 cut(s) 747
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 747
BssECI CCNNGG 3 cut(s) 247, 360, 435
BssNI GRCGYC 2 cut(s) 93, 426
Bst4CI ACNGT 4 cut(s) 214, 350, 719, 876
BstACI GRCGYC 2 cut(s) 93, 426
BstBAI YACGTR 1 cut(s) 863
BstC8I GCNNGC 5 cut(s) 220, 257, 443, 745, 749
BstDEI CTNAG 2 cut(s) 266, 685
BstDSI CCRYGG 1 cut(s) 247
BstF5I GGATG 1 cut(s) 285
BstH2I RGCGCY 2 cut(s) 273, 530
BstHHI GCGC 4 cut(s) 272, 379, 529, 736
BstMAI GTCTC 6 cut(s) 114, 340, 356, 450, 536, 855
BstMCI CGRYCG 1 cut(s) 51
BstSCI CCNGG 2 cut(s) 112, 365
BstSFI CTRYAG 1 cut(s) 930
BstV1I GCAGC 4 cut(s) 479, 485, 913, 916
BstX2I RGATCY 2 cut(s) 450, 805
BstYI RGATCY 2 cut(s) 450, 805
BsuRI GGCC 2 cut(s) 365, 747
BtgI CCRYGG 1 cut(s) 247
BtrI CACGTC 1 cut(s) 165
BtsCI GGATG 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 406
Cac8I GCNNGC 5 cut(s) 220, 257, 443, 745, 749
CaiI CAGNNNCTG 1 cut(s) 772
CfoI GCGC 4 cut(s) 272, 379, 529, 736
Cfr10I RCCGGY 1 cut(s) 747
Cfr13I GGNCC 3 cut(s) 364, 615, 706
CpoI CGGWCCG 1 cut(s) 706
CseI GACGC 6 cut(s) 101, 398, 407, 415, 749, 878
Csp6I GTAC 3 cut(s) 382, 487, 860
CspCI CAANNNNNGTGG 2 cut(s) 704, 739
CspI CGGWCCG 1 cut(s) 706
CviAII CATG 5 cut(s) 562, 595, 676, 827, 854
CviQI GTAC 3 cut(s) 382, 487, 860
DdeI CTNAG 2 cut(s) 266, 685
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
EaeI YGGCCR 1 cut(s) 745
Ecl136II GAGCTC 1 cut(s) 628
Eco24I GRGCYC 1 cut(s) 630
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 2 cut(s) 615, 706
Eco53kI GAGCTC 1 cut(s) 628
Eco88I CYCGRG 2 cut(s) 359, 434
EcoICRI GAGCTC 1 cut(s) 628
EcoRV GATATC 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 630
Esp3I CGTCTC 2 cut(s) 114, 356
FaeI CATG 5 cut(s) 565, 598, 679, 830, 857
FaiI YATR 9 cut(s) 548, 563, 581, 596, 677, 753, 828, 855, 897
FaqI GGGAC 1 cut(s) 695
FatI CATG 5 cut(s) 561, 594, 675, 826, 853
FauI CCCGC 5 cut(s) 74, 227, 331, 494, 596
FblI GTMKAC 2 cut(s) 867, 912
Fnu4HI GCNGC 8 cut(s) 253, 273, 493, 496, 499, 770, 927, 930
FokI GGATG 1 cut(s) 272
FriOI GRGCYC 1 cut(s) 630
Fsp4HI GCNGC 8 cut(s) 253, 273, 493, 496, 499, 770, 927, 930
FspBI CTAG 3 cut(s) 332, 474, 887
GlaI GCGC 4 cut(s) 271, 378, 528, 735
GluI GCNGC 8 cut(s) 253, 273, 493, 496, 499, 770, 927, 930
GsaI CCCAGC 1 cut(s) 776
HaeII RGCGCY 2 cut(s) 273, 530
HaeIII GGCC 2 cut(s) 365, 747
HapII CCGG 5 cut(s) 114, 367, 374, 709, 748
HgaI GACGC 6 cut(s) 101, 398, 407, 415, 749, 878
HhaI GCGC 4 cut(s) 272, 379, 529, 736
Hin1I GRCGYC 2 cut(s) 93, 426
Hin1II CATG 5 cut(s) 565, 598, 679, 830, 857
Hin6I GCGC 4 cut(s) 270, 377, 527, 734
HinP1I GCGC 4 cut(s) 270, 377, 527, 734
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HinfI GANTC 2 cut(s) 174, 607
HpaII CCGG 5 cut(s) 114, 367, 374, 709, 748
HphI GGTGA 2 cut(s) 301, 799
Hpy166II GTNNAC 5 cut(s) 40, 430, 817, 868, 913
Hpy188I TCNGA 7 cut(s) 154, 182, 455, 612, 625, 800, 810
Hpy8I GTNNAC 5 cut(s) 40, 430, 817, 868, 913
Hpy99I CGWCG 5 cut(s) 56, 95, 218, 706, 743
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 4 cut(s) 214, 350, 719, 876
HpyCH4IV ACGT 2 cut(s) 164, 862
HpyCH4V TGCA 2 cut(s) 565, 932
HpyF3I CTNAG 2 cut(s) 266, 685
HpySE526I ACGT 2 cut(s) 164, 862
Hsp92I GRCGYC 2 cut(s) 93, 426
Hsp92II CATG 5 cut(s) 565, 598, 679, 830, 857
HspAI GCGC 4 cut(s) 270, 377, 527, 734
KroI GCCGGC 1 cut(s) 747
KroNI GCCGGC 1 cut(s) 749
LmnI GCTCC 4 cut(s) 198, 264, 305, 625
Lsp1109I GCAGC 4 cut(s) 479, 485, 913, 916
MaeI CTAG 3 cut(s) 332, 474, 887
MaeII ACGT 2 cut(s) 164, 862
MaeIII GTNAC 1 cut(s) 835
MboII GAAGA 1 cut(s) 169
MflI RGATCY 2 cut(s) 450, 805
MhlI GDGCHC 2 cut(s) 630, 669
MluCI AATT 3 cut(s) 8, 343, 632
MmeI TCCRAC 3 cut(s) 205, 534, 603
MroNI GCCGGC 1 cut(s) 747
MseI TTAA 2 cut(s) 414, 948
MspA1I CMGCKG 2 cut(s) 603, 769
MspI CCGG 5 cut(s) 114, 367, 374, 709, 748
MspR9I CCNGG 2 cut(s) 114, 367
NaeI GCCGGC 1 cut(s) 749
NciI CCSGG 2 cut(s) 114, 367
NgoMIV GCCGGC 1 cut(s) 747
NlaIII CATG 5 cut(s) 565, 598, 679, 830, 857
NlaIV GGNNCC 6 cut(s) 260, 452, 558, 713, 807, 893
NmeAIII GCCGAG 1 cut(s) 503
NmuCI GTSAC 1 cut(s) 835
PcsI WCGNNNNNNNCGW 2 cut(s) 178, 210
PdiI GCCGGC 1 cut(s) 749
PfeI GAWTC 2 cut(s) 174, 607
PflFI GACNNNGTC 1 cut(s) 619
PflMI CCANNNNNTGG 2 cut(s) 165, 772
PkrI GCNGC 8 cut(s) 254, 274, 494, 497, 500, 771, 928, 931
Ple19I CGATCG 1 cut(s) 51
Ppu21I YACGTR 1 cut(s) 863
Psp124BI GAGCTC 1 cut(s) 630
PspFI CCCAGC 1 cut(s) 772
PspN4I GGNNCC 6 cut(s) 260, 452, 558, 713, 807, 893
PspPI GGNCC 3 cut(s) 364, 615, 706
PstI CTGCAG 1 cut(s) 934
PstNI CAGNNNCTG 1 cut(s) 772
PsuI RGATCY 2 cut(s) 450, 805
PsyI GACNNNGTC 1 cut(s) 619
PvuI CGATCG 1 cut(s) 51
RsaI GTAC 3 cut(s) 383, 488, 861
RsaNI GTAC 3 cut(s) 382, 487, 860
Rsr2I CGGWCCG 1 cut(s) 706
RsrII CGGWCCG 1 cut(s) 706
SacI GAGCTC 1 cut(s) 630
SaqAI TTAA 2 cut(s) 414, 948
SatI GCNGC 8 cut(s) 253, 273, 493, 496, 499, 770, 927, 930
Sau96I GGNCC 3 cut(s) 364, 615, 706
ScrFI CCNGG 2 cut(s) 114, 367
SduI GDGCHC 2 cut(s) 630, 669
SfcI CTRYAG 1 cut(s) 930
SinI GGWCC 2 cut(s) 615, 706
Sse9I AATT 3 cut(s) 8, 343, 632
SspI AATATT 1 cut(s) 901
SspMI CTAG 3 cut(s) 332, 474, 887
SstI GAGCTC 1 cut(s) 630
StyD4I CCNGG 2 cut(s) 112, 365
TaaI ACNGT 4 cut(s) 214, 350, 719, 876
TaiI ACGT 2 cut(s) 167, 865
TaqI TCGA 4 cut(s) 51, 669, 701, 738
TasI AATT 3 cut(s) 8, 343, 632
TauI GCSGC 4 cut(s) 255, 275, 498, 772
TfiI GAWTC 2 cut(s) 174, 607
Tru1I TTAA 2 cut(s) 414, 948
Tru9I TTAA 2 cut(s) 414, 948
TscAI CASTG 1 cut(s) 413
TseFI GTSAC 1 cut(s) 835
TseI GCWGC 4 cut(s) 492, 498, 926, 929
Tsp45I GTSAC 1 cut(s) 835
TspDTI ATGAA 2 cut(s) 563, 599
TspGWI ACGGA 2 cut(s) 656, 719
TspRI CASTG 1 cut(s) 413
Tth111I GACNNNGTC 1 cut(s) 619
Van91I CCANNNNNTGG 2 cut(s) 165, 772
VpaK11BI GGWCC 2 cut(s) 615, 706
XapI RAATTY 2 cut(s) 8, 632
XmiI GTMKAC 2 cut(s) 867, 912
XspI CTAG 3 cut(s) 332, 474, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.