MD02G1198300.v1.1

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015105
Physical Location & Seq
Forward (+)
19452996 .. 19453952
957 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1198300.v1.1.491

Sequence Viewer

Length: 957 bp
ATGTCACAATTCGACCCCTACACCCACCTCCACATAGTACACGACCCCGCCACCGACACCCTCACCCGCCCCGCCGCCGCCACTATACCCGCCAACCCGGACCCCGCCCCAGGCGACCCGGTCGTCTCTAAAGACGTGACACTCAACCCGGAAACCAAAACCTGGATCCGGATCTTCCGACCCGCCAAGCTTCCCTCCAACGACAACACCGTGGCCCGCCTCCCCCTCATCATCTACTTCCACCACGGCGCCTGGATATTCCTCTCCGCCGCCGACTCCACCGCGCACACTAACTGCTCTCAGATCACCTCCGAAATTCCCGCCATCATCGTCTCCGTCAACTACCGCCTGGCGCCCGAAACCCGGCTCCCGGGCCAGTACCATGACGCCATCGACGCAATCAACTGGGTCAGGGCCCAGGCCCAGGACCCGAAAGGCGAGACCTGGATCCGAGACTACGCCGACGTATCCAGGTGCTATCTCTACGGTTGCGGATGCGGGGGCAACATCGTCTTTTTCTCCGGATTAAAAGCGTACCAGCTCCAGCTAGAGCCGTTGAAGATCTCCGGGATTATTATGAACCAGCCGATGTTTGGTGGGGTCCAGCGGACTAAGTCGGAGCTGCGATTGGCTGTGGACCAACTGTTGCCTTTGCCTGCGCTCGACCTCATGTGGGACCTCGCGCTGCCGAAATCAACAGACAGGAACCATCGGTACTGTAATCCGATGGCGGATGGGGCCCACAAGGCGATGATCAAAGGGCTGGGGCGGTTTTTGGTGATTGGGTTCGGTGGGGACCCGATGGTCGACCGGCAGCAGGAGTTTGTGACGATGCTGGTGGGTTGTGGGGTCAGGGTTGATGCGCGGTTCGATGACGTGGGGTTCCATAATATTGACTTCGTTGACACCAGGCGGGCTGCTGCCGTTTTGAACATTGTCAAGGAGTTTATCATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

319

Amino Acids

35.29

Weight (kDa)

6.35

Isoelectric Point (pI)

29.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 69 - 184 1.2e-10 BD-FAE
Abhydrolase_3 PF07859 77 - 297 7e-42 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015105)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45610
fragaria_vesca FvH4_7g12520
malus_domestica MD02G1198300.v1.1
prunus_persica Prupe.2G149700_v2.0.a1
pyrus_communis pycom02g16190
rosa_chinensis RchiOBHm_Chr1g0351421
rosa_laevigata RLG00000028408
rosa_multiflora Rmu_sc0000664.1_g000007 Rmu_sc0005320.1_g000004
rosa_roxburghii Rroxscaffold_4G00303840
rosa_rugosa Rorug01G0218000
rosa_samantha Rh1AG232500 Rh1BG203300 Rh1CG217200 Rh1DG230300
rosa_wichuraiana Rw1G020250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 122, 803
AccB1I GGYRCC 2 cut(s) 248, 352
AccB7I CCANNNNNTGG 1 cut(s) 162
AccI GTMKAC 1 cut(s) 807
AccII CGCG 3 cut(s) 284, 683, 865
AccIII TCCGGA 2 cut(s) 168, 521
AclWI GGATC 5 cut(s) 160, 173, 179, 442, 455
AcsI RAATTY 1 cut(s) 315
AcyI GRCGYC 3 cut(s) 249, 353, 387
AfaI GTAC 4 cut(s) 39, 380, 536, 716
AfiI CCNNNNNNNGG 9 cut(s) 110, 162, 168, 363, 370, 424, 593, 673, 817
AgsI TTSAA 2 cut(s) 559, 931
AjiI CACGTC 2 cut(s) 136, 877
AjnI CCWGG 9 cut(s) 109, 161, 251, 348, 417, 423, 443, 470, 908
AluBI AGCT 4 cut(s) 190, 541, 547, 622
AluI AGCT 4 cut(s) 190, 541, 547, 622
Alw26I GTCTC 4 cut(s) 130, 337, 434, 447
AlwI GGATC 5 cut(s) 160, 173, 179, 442, 455
Ama87I CYCGRG 1 cut(s) 370
Aor13HI TCCGGA 2 cut(s) 168, 521
AoxI GGCC 5 cut(s) 213, 373, 414, 420, 738
ApaI GGGCCC 2 cut(s) 418, 742
ApeKI GCWGC 5 cut(s) 622, 685, 814, 917, 920
ApoI RAATTY 1 cut(s) 315
AspLEI GCGC 6 cut(s) 251, 286, 355, 661, 685, 865
AsuC2I CCSGG 7 cut(s) 98, 119, 149, 364, 371, 372, 568
AsuHPI GGTGA 3 cut(s) 55, 298, 790
AvaI CYCGRG 1 cut(s) 370
AvaII GGWCC 6 cut(s) 100, 427, 601, 637, 676, 796
BaeGI GKGCMC 2 cut(s) 418, 742
BamHI GGATCC 2 cut(s) 165, 447
BanI GGYRCC 2 cut(s) 248, 352
BanII GRGCYC 2 cut(s) 418, 742
BbvI GCAGC 5 cut(s) 609, 672, 826, 904, 907
BccI CCATC 6 cut(s) 332, 398, 717, 721, 728, 796
BceAI ACGGC 3 cut(s) 262, 538, 908
BciT130I CCWGG 9 cut(s) 111, 163, 253, 350, 419, 425, 445, 472, 910
BciVI GTATCC 1 cut(s) 478
BclI TGATCA 1 cut(s) 753
BcnI CCSGG 7 cut(s) 98, 119, 149, 364, 371, 372, 568
BcoDI GTCTC 4 cut(s) 130, 337, 434, 447
BfaI CTAG 1 cut(s) 548
BfoI RGCGCY 2 cut(s) 252, 356
BfuI GTATCC 1 cut(s) 478
BglI GCCNNNNNGGC 1 cut(s) 746
BglII AGATCT 1 cut(s) 561
BisI GCNGC 8 cut(s) 75, 78, 270, 623, 686, 815, 918, 921
BlsI GCNGC 8 cut(s) 76, 79, 271, 624, 687, 816, 919, 922
Bme18I GGWCC 6 cut(s) 100, 427, 601, 637, 676, 796
BmeT110I CYCGRG 1 cut(s) 370
BmgBI CACGTC 2 cut(s) 136, 877
BmrI ACTGGG 1 cut(s) 415
BmsI GCATC 3 cut(s) 485, 822, 850
BmuI ACTGGG 1 cut(s) 415
BpmI CTGGAG 1 cut(s) 527
BpuMI CCSGG 7 cut(s) 98, 119, 149, 364, 371, 372, 568
BsaBI GATNNNNATC 1 cut(s) 170
BsaHI GRCGYC 3 cut(s) 249, 353, 387
BsaI GGTCTC 1 cut(s) 434
BsaJI CCNNGG 6 cut(s) 109, 210, 244, 370, 417, 423
BsaWI WCCGGW 2 cut(s) 168, 521
Bsc4I CCNNNNNNNGG 9 cut(s) 110, 162, 168, 363, 370, 424, 593, 673, 817
Bse118I RCCGGY 1 cut(s) 810
Bse1I ACTGG 2 cut(s) 376, 410
Bse8I GATNNNNATC 1 cut(s) 170
BseAI TCCGGA 2 cut(s) 168, 521
BseBI CCWGG 9 cut(s) 111, 163, 253, 350, 419, 425, 445, 472, 910
BseDI CCNNGG 6 cut(s) 109, 210, 244, 370, 417, 423
BseGI GGATG 2 cut(s) 500, 739
BseJI GATNNNNATC 1 cut(s) 170
BseLI CCNNNNNNNGG 9 cut(s) 110, 162, 168, 363, 370, 424, 593, 673, 817
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 2 cut(s) 376, 410
BseSI GKGCMC 2 cut(s) 418, 742
BseXI GCAGC 5 cut(s) 609, 672, 826, 904, 907
BseYI CCCAGC 1 cut(s) 763
Bsh1236I CGCG 3 cut(s) 284, 683, 865
Bsh1285I CGRYCG 2 cut(s) 123, 811
BshFI GGCC 5 cut(s) 215, 375, 416, 422, 740
BshNI GGYRCC 2 cut(s) 248, 352
BsiEI CGRYCG 2 cut(s) 123, 811
BsiHKCI CYCGRG 1 cut(s) 370
BsiSI CCGG 9 cut(s) 98, 119, 149, 169, 364, 371, 522, 567, 811
BslFI GGGAC 2 cut(s) 689, 809
BslI CCNNNNNNNGG 9 cut(s) 110, 162, 168, 363, 370, 424, 593, 673, 817
BsmAI GTCTC 4 cut(s) 130, 337, 434, 447
BsmBI CGTCTC 2 cut(s) 130, 337
BsmFI GGGAC 2 cut(s) 689, 809
BsnI GGCC 5 cut(s) 215, 375, 416, 422, 740
Bso31I GGTCTC 1 cut(s) 434
BsoBI CYCGRG 1 cut(s) 370
Bsp120I GGGCCC 2 cut(s) 414, 738
Bsp1286I GDGCHC 2 cut(s) 418, 742
Bsp13I TCCGGA 2 cut(s) 168, 521
Bsp143I GATC 6 cut(s) 165, 171, 303, 447, 561, 753
BspANI GGCC 5 cut(s) 215, 375, 416, 422, 740
BspCNI CTCAG 1 cut(s) 313
BspEI TCCGGA 2 cut(s) 168, 521
BspFNI CGCG 3 cut(s) 284, 683, 865
BspPI GGATC 5 cut(s) 160, 173, 179, 442, 455
BspT107I GGYRCC 2 cut(s) 248, 352
BspTNI GGTCTC 1 cut(s) 434
BsrFI RCCGGY 1 cut(s) 810
BsrI ACTGG 2 cut(s) 376, 410
BssAI RCCGGY 1 cut(s) 810
BssECI CCNNGG 6 cut(s) 109, 210, 244, 370, 417, 423
BssMI GATC 6 cut(s) 165, 171, 303, 447, 561, 753
BssNI GRCGYC 3 cut(s) 249, 353, 387
Bst2UI CCWGG 9 cut(s) 111, 163, 253, 350, 419, 425, 445, 472, 910
Bst4CI ACNGT 4 cut(s) 211, 488, 645, 719
BstACI GRCGYC 3 cut(s) 249, 353, 387
BstC8I GCNNGC 3 cut(s) 217, 657, 915
BstDEI CTNAG 2 cut(s) 300, 612
BstDSI CCRYGG 2 cut(s) 210, 244
BstF5I GGATG 2 cut(s) 500, 739
BstFNI CGCG 3 cut(s) 284, 683, 865
BstH2I RGCGCY 2 cut(s) 252, 356
BstHHI GCGC 6 cut(s) 251, 286, 355, 661, 685, 865
BstKTI GATC 6 cut(s) 168, 174, 306, 450, 564, 756
BstMAI GTCTC 4 cut(s) 130, 337, 434, 447
BstMBI GATC 6 cut(s) 165, 171, 303, 447, 561, 753
BstMCI CGRYCG 2 cut(s) 123, 811
BstMWI GCNNNNNNNGC 3 cut(s) 395, 737, 746
BstNI CCWGG 9 cut(s) 111, 163, 253, 350, 419, 425, 445, 472, 910
BstSLI GKGCMC 2 cut(s) 418, 742
BstUI CGCG 3 cut(s) 284, 683, 865
BstV1I GCAGC 5 cut(s) 609, 672, 826, 904, 907
BstX2I RGATCY 4 cut(s) 165, 171, 447, 561
BstYI RGATCY 4 cut(s) 165, 171, 447, 561
BsuI GTATCC 1 cut(s) 478
BsuRI GGCC 5 cut(s) 215, 375, 416, 422, 740
BtgI CCRYGG 2 cut(s) 210, 244
BtgZI GCGATG 1 cut(s) 764
BtrI CACGTC 2 cut(s) 136, 877
BtsCI GGATG 2 cut(s) 500, 739
Cac8I GCNNGC 3 cut(s) 217, 657, 915
CfoI GCGC 6 cut(s) 251, 286, 355, 661, 685, 865
Cfr10I RCCGGY 1 cut(s) 810
Cfr9I CCCGGG 1 cut(s) 370
CseI GACGC 2 cut(s) 395, 404
Csp6I GTAC 4 cut(s) 38, 379, 535, 715
CviAII CATG 2 cut(s) 383, 670
CviQI GTAC 4 cut(s) 38, 379, 535, 715
DdeI CTNAG 2 cut(s) 300, 612
DinI GGCGCC 2 cut(s) 250, 354
DpnI GATC 6 cut(s) 167, 173, 305, 449, 563, 755
DpnII GATC 6 cut(s) 165, 171, 303, 447, 561, 753
DrdI GACNNNNNNGTC 2 cut(s) 122, 803
DseDI GACNNNNNNGTC 2 cut(s) 122, 803
EciI GGCGGA 2 cut(s) 256, 746
Eco24I GRGCYC 2 cut(s) 418, 742
Eco31I GGTCTC 1 cut(s) 434
Eco47I GGWCC 6 cut(s) 100, 427, 601, 637, 676, 796
Eco88I CYCGRG 1 cut(s) 370
EcoO109I RGGNCCY 5 cut(s) 414, 427, 676, 738, 796
EcoRII CCWGG 9 cut(s) 109, 161, 251, 348, 417, 423, 443, 470, 908
EcoT38I GRGCYC 2 cut(s) 418, 742
EgeI GGCGCC 2 cut(s) 250, 354
EheI GGCGCC 2 cut(s) 250, 354
Esp3I CGTCTC 2 cut(s) 130, 337
FaeI CATG 2 cut(s) 386, 673
FaiI YATR 6 cut(s) 35, 86, 384, 578, 671, 888
FaqI GGGAC 2 cut(s) 689, 809
FatI CATG 2 cut(s) 382, 669
FbaI TGATCA 1 cut(s) 753
FblI GTMKAC 1 cut(s) 807
Fnu4HI GCNGC 8 cut(s) 75, 78, 270, 623, 686, 815, 918, 921
FokI GGATG 2 cut(s) 507, 746
FriOI GRGCYC 2 cut(s) 418, 742
Fsp4HI GCNGC 8 cut(s) 75, 78, 270, 623, 686, 815, 918, 921
FspBI CTAG 1 cut(s) 548
GlaI GCGC 6 cut(s) 250, 285, 354, 660, 684, 864
GluI GCNGC 8 cut(s) 75, 78, 270, 623, 686, 815, 918, 921
GsaI CCCAGC 1 cut(s) 767
GsuI CTGGAG 1 cut(s) 527
HaeII RGCGCY 2 cut(s) 252, 356
HaeIII GGCC 5 cut(s) 215, 375, 416, 422, 740
HapII CCGG 9 cut(s) 98, 119, 149, 169, 364, 371, 522, 567, 811
HgaI GACGC 2 cut(s) 395, 404
HhaI GCGC 6 cut(s) 251, 286, 355, 661, 685, 865
Hin1I GRCGYC 3 cut(s) 249, 353, 387
Hin1II CATG 2 cut(s) 386, 673
Hin6I GCGC 6 cut(s) 249, 284, 353, 659, 683, 863
HinP1I GCGC 6 cut(s) 249, 284, 353, 659, 683, 863
HincII GTYRAC 3 cut(s) 340, 808, 904
HindII GTYRAC 3 cut(s) 340, 808, 904
HindIII AAGCTT 1 cut(s) 188
HinfI GANTC 1 cut(s) 275
HpaII CCGG 9 cut(s) 98, 119, 149, 169, 364, 371, 522, 567, 811
HphI GGTGA 3 cut(s) 55, 298, 790
Hpy166II GTNNAC 5 cut(s) 40, 340, 637, 808, 904
Hpy188I TCNGA 6 cut(s) 179, 303, 313, 452, 619, 726
Hpy188III TCNNGA 2 cut(s) 169, 522
Hpy8I GTNNAC 5 cut(s) 40, 340, 637, 808, 904
Hpy99I CGWCG 2 cut(s) 398, 467
HpyCH4III ACNGT 4 cut(s) 211, 488, 645, 719
HpyCH4IV ACGT 3 cut(s) 135, 465, 876
HpyF10VI GCNNNNNNNGC 3 cut(s) 395, 737, 746
HpyF3I CTNAG 2 cut(s) 300, 612
HpySE526I ACGT 3 cut(s) 135, 465, 876
Hsp92I GRCGYC 3 cut(s) 249, 353, 387
Hsp92II CATG 2 cut(s) 386, 673
HspAI GCGC 6 cut(s) 249, 284, 353, 659, 683, 863
KasI GGCGCC 2 cut(s) 248, 352
KflI GGGWCCC 1 cut(s) 796
Kpn2I TCCGGA 2 cut(s) 168, 521
Ksp22I TGATCA 1 cut(s) 753
Kzo9I GATC 6 cut(s) 165, 171, 303, 447, 561, 753
LmnI GCTCC 3 cut(s) 372, 546, 619
Lsp1109I GCAGC 5 cut(s) 609, 672, 826, 904, 907
LweI GCATC 3 cut(s) 485, 822, 850
MaeI CTAG 1 cut(s) 548
MaeII ACGT 3 cut(s) 135, 465, 876
MaeIII GTNAC 3 cut(s) 3, 136, 826
MalI GATC 6 cut(s) 167, 173, 305, 449, 563, 755
MboI GATC 6 cut(s) 165, 171, 303, 447, 561, 753
MboII GAAGA 2 cut(s) 166, 571
MflI RGATCY 4 cut(s) 165, 171, 447, 561
MhlI GDGCHC 2 cut(s) 418, 742
MluCI AATT 2 cut(s) 8, 315
Mly113I GGCGCC 2 cut(s) 249, 353
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 3 cut(s) 202, 222, 597
MnlI CCTC 9 cut(s) 38, 71, 205, 230, 236, 272, 319, 677, 689
MroI TCCGGA 2 cut(s) 168, 521
MseI TTAA 1 cut(s) 527
MspA1I CMGCKG 1 cut(s) 607
MspI CCGG 9 cut(s) 98, 119, 149, 169, 364, 371, 522, 567, 811
MvaI CCWGG 9 cut(s) 111, 163, 253, 350, 419, 425, 445, 472, 910
MvnI CGCG 3 cut(s) 284, 683, 865
MwoI GCNNNNNNNGC 3 cut(s) 395, 737, 746
NarI GGCGCC 2 cut(s) 249, 353
NciI CCSGG 7 cut(s) 98, 119, 149, 364, 371, 372, 568
NdeII GATC 6 cut(s) 165, 171, 303, 447, 561, 753
NlaIII CATG 2 cut(s) 386, 673
NmuCI GTSAC 3 cut(s) 3, 136, 826
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PflFI GACNNNGTC 2 cut(s) 119, 613
PflMI CCANNNNNTGG 1 cut(s) 162
PfoI TCCNGGA 1 cut(s) 566
PkrI GCNGC 8 cut(s) 76, 79, 271, 624, 687, 816, 919, 922
PleI GAGTC 1 cut(s) 269
PluTI GGCGCC 2 cut(s) 252, 356
PpsI GAGTC 1 cut(s) 269
PpuMI RGGWCCY 3 cut(s) 427, 676, 796
Psp5II RGGWCCY 3 cut(s) 427, 676, 796
Psp6I CCWGG 9 cut(s) 109, 161, 251, 348, 417, 423, 443, 470, 908
PspFI CCCAGC 1 cut(s) 763
PspGI CCWGG 9 cut(s) 109, 161, 251, 348, 417, 423, 443, 470, 908
PspOMI GGGCCC 2 cut(s) 414, 738
PspPPI RGGWCCY 3 cut(s) 427, 676, 796
PsrI GAACNNNNNNTAC 2 cut(s) 698, 730
PsuI RGATCY 4 cut(s) 165, 171, 447, 561
PsyI GACNNNGTC 2 cut(s) 119, 613
RsaI GTAC 4 cut(s) 39, 380, 536, 716
RsaNI GTAC 4 cut(s) 38, 379, 535, 715
SalI GTCGAC 1 cut(s) 806
SaqAI TTAA 1 cut(s) 527
SatI GCNGC 8 cut(s) 75, 78, 270, 623, 686, 815, 918, 921
Sau3AI GATC 6 cut(s) 165, 171, 303, 447, 561, 753
SchI GAGTC 1 cut(s) 269
SduI GDGCHC 2 cut(s) 418, 742
SfaNI GCATC 3 cut(s) 485, 822, 850
SfoI GGCGCC 2 cut(s) 250, 354
SinI GGWCC 6 cut(s) 100, 427, 601, 637, 676, 796
SmaI CCCGGG 1 cut(s) 372
Sse9I AATT 2 cut(s) 8, 315
SspDI GGCGCC 2 cut(s) 248, 352
SspI AATATT 1 cut(s) 892
SspMI CTAG 1 cut(s) 548
TaaI ACNGT 4 cut(s) 211, 488, 645, 719
TaiI ACGT 3 cut(s) 138, 468, 879
TaqI TCGA 5 cut(s) 12, 393, 663, 807, 870
TasI AATT 2 cut(s) 8, 315
TatI WGTACW 1 cut(s) 37
TauI GCSGC 3 cut(s) 77, 80, 272
Tru1I TTAA 1 cut(s) 527
Tru9I TTAA 1 cut(s) 527
TseFI GTSAC 3 cut(s) 3, 136, 826
TseI GCWGC 5 cut(s) 622, 685, 814, 917, 920
Tsp45I GTSAC 3 cut(s) 3, 136, 826
TspDTI ATGAA 1 cut(s) 593
TspGWI ACGGA 1 cut(s) 325
TspMI CCCGGG 1 cut(s) 370
Tth111I GACNNNGTC 2 cut(s) 119, 613
Van91I CCANNNNNTGG 1 cut(s) 162
VpaK11BI GGWCC 6 cut(s) 100, 427, 601, 637, 676, 796
XapI RAATTY 1 cut(s) 315
XcmI CCANNNNNNNNNTGG 1 cut(s) 590
XmaI CCCGGG 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 807
XspI CTAG 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.