RchiOBHm_Chr1g0351421

alpha/beta hydrolase fold

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0015105
Physical Location & Seq
Reverse (-)
44678680 .. 44679649
970 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57719

Sequence Viewer

Length: 969 bp
ATGTCAAAATTTGATCCTTACACCCACCTCAACATTGTCCACAACCCGGTCGACGACACCTTGACCCGCCTCATCAGCCTCCCCACCACCGACGCCAACCCGAACCCGTCTCCCGGCGACCCGGTCGCCTCCAAAGACCTAACCCTCAACTCGGAAACCAAGACGTGGGTGCGAATCTTCCGACCCTCTAAACTCCCCTCCAACGACAACACCGTTGCCCGCCTCCCCATCATCATCTACTTCCACCACGGCGGCTGGCTCCTCCTCAGCGCCGCCGACTCCTCCGTCCACTCCAACTGCTCCTTCATCACCAGCCAAGTTCCCGCCATTCTCGTCTCCGTAAATTACCGTCTCGCCCCCGAGGCCCGGCTCCCGGCCCAATACGACGATGCCATGGAAGCCATCCACTGGGTCAAGAAGCAGGGCCTCGACCCCGGGGGCGAGCAGTGGATCCGAGACTACGGTGACCTTTCTAGGTGCTACTTGTACGGCTGCGGCTGCGGGGGCAATATCGTCTTTTTCTCGGCTCTCAAAGCCTATGAAATGAAGCTGGATTTGGAGCCCATGCAGATATCTGGGATCATTATGAACCAACCCATGTTCGGCGGGATCCAGAGGACCAAGTCGGAGCTGAAATTCGCTACGGACCAGCTCTTGCCTTTGCCTGTGCTCGACCTCATGTGGGACCTAGCACTGCCGAAATCAACGGACCGGAACCATAGGTATTGTAGTCCGGCGCTCGACGCTGGCCGGTATAAAGAGATGATCCAACGGCTGGGGCGGTGTTTAGTGATCGGATTCGGAGGAGATCCGATGGTGGACAGGCAACAGGAGTTTGTGACGATGTTGGTATCTCAGGGGATACGTGTAGACGCGCGGTTTGATAATCTAGGGTTCCATAATATTGATTTTGTAGACCAGAGAAGGGCTGCTGAGGTTTTGGACATTGTTAAGGAGTTTATCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

322

Amino Acids

36.2

Weight (kDa)

5.9

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 70 - 186 2e-09 BD-FAE
Abhydrolase_3 PF07859 78 - 301 1.1e-40 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015105)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G45610
fragaria_vesca FvH4_7g12520
malus_domestica MD02G1198300.v1.1
prunus_persica Prupe.2G149700_v2.0.a1
pyrus_communis pycom02g16190
rosa_chinensis RchiOBHm_Chr1g0351421
rosa_laevigata RLG00000028408
rosa_multiflora Rmu_sc0000664.1_g000007 Rmu_sc0005320.1_g000004
rosa_roxburghii Rroxscaffold_4G00303840
rosa_rugosa Rorug01G0218000
rosa_samantha Rh1AG232500 Rh1BG203300 Rh1CG217200 Rh1DG230300
rosa_wichuraiana Rw1G020250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AccB7I CCANNNNNTGG 3 cut(s) 165, 408, 775
AccI GTMKAC 3 cut(s) 51, 870, 915
AccII CGCG 2 cut(s) 875, 877
AclWI GGATC 8 cut(s) 8, 445, 458, 587, 604, 617, 760, 803
AcoI YGGCCR 1 cut(s) 748
AcsI RAATTY 2 cut(s) 8, 635
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 1 cut(s) 488
AflIII ACRYGT 1 cut(s) 865
AjiI CACGTC 1 cut(s) 165
AjuI GAANNNNNNNTTGG 4 cut(s) 287, 319, 539, 571
AluBI AGCT 3 cut(s) 550, 631, 652
AluI AGCT 3 cut(s) 550, 631, 652
Alw21I GWGCWC 1 cut(s) 672
Alw26I GTCTC 4 cut(s) 114, 340, 356, 450
AlwI GGATC 8 cut(s) 8, 445, 458, 587, 604, 617, 760, 803
Ama87I CYCGRG 2 cut(s) 359, 434
AoxI GGCC 4 cut(s) 363, 375, 424, 748
ApeKI GCWGC 3 cut(s) 492, 498, 929
ApoI RAATTY 2 cut(s) 8, 635
ArsI GACNNNNNNTTYG 2 cut(s) 863, 895
AspLEI GCGC 3 cut(s) 272, 739, 877
AspS9I GGNCC 7 cut(s) 364, 376, 424, 618, 646, 685, 709
AsuC2I CCSGG 7 cut(s) 47, 114, 122, 367, 374, 435, 436
AsuHPI GGTGA 2 cut(s) 301, 476
AvaI CYCGRG 2 cut(s) 359, 434
AvaII GGWCC 4 cut(s) 618, 646, 685, 709
BamHI GGATCC 2 cut(s) 450, 609
BanII GRGCYC 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 672
BbvCI CCTCAGC 2 cut(s) 266, 933
BbvI GCAGC 3 cut(s) 479, 485, 916
BccI CCATC 3 cut(s) 236, 410, 808
BceAI ACGGC 3 cut(s) 265, 505, 788
BciVI GTATCC 1 cut(s) 855
BcnI CCSGG 7 cut(s) 47, 114, 122, 367, 374, 435, 436
BcoDI GTCTC 4 cut(s) 114, 340, 356, 450
BfaI CTAG 3 cut(s) 474, 689, 890
BfoI RGCGCY 2 cut(s) 273, 740
BfuI GTATCC 1 cut(s) 855
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 6 cut(s) 253, 273, 493, 496, 499, 930
BlsI GCNGC 6 cut(s) 254, 274, 494, 497, 500, 931
Bme1390I CCNGG 7 cut(s) 47, 114, 122, 367, 374, 435, 436
Bme18I GGWCC 4 cut(s) 618, 646, 685, 709
BmeT110I CYCGRG 2 cut(s) 359, 434
BmgBI CACGTC 1 cut(s) 165
BmgT120I GGNCC 7 cut(s) 364, 376, 424, 618, 646, 685, 709
BmiI GGNNCC 8 cut(s) 260, 371, 452, 561, 611, 686, 716, 896
BmrFI CCNGG 7 cut(s) 47, 114, 122, 367, 374, 435, 436
BmrI ACTGGG 1 cut(s) 418
BmsI GCATC 1 cut(s) 379
BmuI ACTGGG 1 cut(s) 418
Bpu10I CCTNAGC 2 cut(s) 266, 933
BpuMI CCSGG 7 cut(s) 47, 114, 122, 367, 374, 435, 436
BsaAI YACGTR 1 cut(s) 866
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 6 cut(s) 247, 360, 393, 433, 434, 435
BsaWI WCCGGW 1 cut(s) 711
Bse118I RCCGGY 1 cut(s) 750
Bse1I ACTGG 1 cut(s) 413
BseDI CCNNGG 6 cut(s) 247, 360, 393, 433, 434, 435
BseGI GGATG 1 cut(s) 402
BseMII CTCAG 3 cut(s) 280, 869, 924
BseNI ACTGG 1 cut(s) 413
BseRI GAGGAG 4 cut(s) 251, 254, 271, 819
BseXI GCAGC 3 cut(s) 479, 485, 916
BseYI CCCAGC 1 cut(s) 775
Bsh1236I CGCG 2 cut(s) 875, 877
Bsh1285I CGRYCG 2 cut(s) 51, 126
BshFI GGCC 4 cut(s) 365, 377, 426, 750
BsiEI CGRYCG 2 cut(s) 51, 126
BsiHKAI GWGCWC 1 cut(s) 672
BsiHKCI CYCGRG 2 cut(s) 359, 434
BsiSI CCGG 9 cut(s) 47, 114, 122, 367, 374, 435, 712, 734, 751
BslFI GGGAC 1 cut(s) 698
BsmAI GTCTC 4 cut(s) 114, 340, 356, 450
BsmBI CGTCTC 3 cut(s) 114, 340, 356
BsmFI GGGAC 1 cut(s) 698
BsnI GGCC 4 cut(s) 365, 377, 426, 750
BsoBI CYCGRG 2 cut(s) 359, 434
Bsp1286I GDGCHC 2 cut(s) 564, 672
Bsp143I GATC 7 cut(s) 13, 450, 579, 609, 765, 792, 808
Bsp19I CCATGG 1 cut(s) 393
BspANI GGCC 4 cut(s) 365, 377, 426, 750
BspCNI CTCAG 3 cut(s) 279, 868, 925
BspFNI CGCG 2 cut(s) 875, 877
BspLI GGNNCC 8 cut(s) 260, 371, 452, 561, 611, 686, 716, 896
BspPI GGATC 8 cut(s) 8, 445, 458, 587, 604, 617, 760, 803
BsrFI RCCGGY 1 cut(s) 750
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 1 cut(s) 750
BssECI CCNNGG 6 cut(s) 247, 360, 393, 433, 434, 435
BssMI GATC 7 cut(s) 13, 450, 579, 609, 765, 792, 808
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 393
Bst4CI ACNGT 3 cut(s) 214, 350, 464
BstACI GRCGYC 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 866
BstC8I GCNNGC 4 cut(s) 220, 257, 443, 748
BstDEI CTNAG 3 cut(s) 266, 855, 933
BstDSI CCRYGG 2 cut(s) 247, 393
BstEII GGTNACC 1 cut(s) 464
BstF5I GGATG 1 cut(s) 402
BstFNI CGCG 2 cut(s) 875, 877
BstH2I RGCGCY 2 cut(s) 273, 740
BstHHI GCGC 3 cut(s) 272, 739, 877
BstKTI GATC 7 cut(s) 16, 453, 582, 612, 768, 795, 811
BstMAI GTCTC 4 cut(s) 114, 340, 356, 450
BstMBI GATC 7 cut(s) 13, 450, 579, 609, 765, 792, 808
BstMCI CGRYCG 2 cut(s) 51, 126
BstMWI GCNNNNNNNGC 7 cut(s) 75, 362, 398, 498, 504, 533, 743
BstPI GGTNACC 1 cut(s) 464
BstSCI CCNGG 7 cut(s) 45, 112, 120, 365, 372, 433, 434
BstUI CGCG 2 cut(s) 875, 877
BstV1I GCAGC 3 cut(s) 479, 485, 916
BstX2I RGATCY 3 cut(s) 450, 609, 808
BstYI RGATCY 3 cut(s) 450, 609, 808
BsuI GTATCC 1 cut(s) 855
BsuRI GGCC 4 cut(s) 365, 377, 426, 750
BtgI CCRYGG 2 cut(s) 247, 393
BtrI CACGTC 1 cut(s) 165
BtsCI GGATG 1 cut(s) 402
BtsI GCAGTG 2 cut(s) 452, 692
BtsIMutI CAGTG 3 cut(s) 406, 452, 692
Cac8I GCNNGC 4 cut(s) 220, 257, 443, 748
CfoI GCGC 3 cut(s) 272, 739, 877
Cfr10I RCCGGY 1 cut(s) 750
Cfr13I GGNCC 7 cut(s) 364, 376, 424, 618, 646, 685, 709
Cfr9I CCCGGG 1 cut(s) 434
CpoI CGGWCCG 1 cut(s) 709
CseI GACGC 3 cut(s) 101, 752, 881
Csp6I GTAC 1 cut(s) 487
CspI CGGWCCG 1 cut(s) 709
CviAII CATG 4 cut(s) 394, 565, 598, 679
CviQI GTAC 1 cut(s) 487
DdeI CTNAG 3 cut(s) 266, 855, 933
DpnI GATC 7 cut(s) 15, 452, 581, 611, 767, 794, 810
DpnII GATC 7 cut(s) 13, 450, 579, 609, 765, 792, 808
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
EaeI YGGCCR 1 cut(s) 748
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 564
Eco32I GATATC 1 cut(s) 573
Eco47I GGWCC 4 cut(s) 618, 646, 685, 709
Eco88I CYCGRG 2 cut(s) 359, 434
Eco91I GGTNACC 1 cut(s) 464
EcoO109I RGGNCCY 2 cut(s) 424, 685
EcoO65I GGTNACC 1 cut(s) 464
EcoRV GATATC 1 cut(s) 573
EcoT14I CCWWGG 1 cut(s) 393
EcoT38I GRGCYC 1 cut(s) 564
ErhI CCWWGG 1 cut(s) 393
Esp3I CGTCTC 3 cut(s) 114, 340, 356
FaeI CATG 4 cut(s) 397, 568, 601, 682
FaiI YATR 9 cut(s) 395, 540, 566, 587, 599, 680, 720, 756, 900
FaqI GGGAC 1 cut(s) 698
FatI CATG 4 cut(s) 393, 564, 597, 678
FauI CCCGC 5 cut(s) 74, 227, 331, 494, 599
FblI GTMKAC 3 cut(s) 51, 870, 915
Fnu4HI GCNGC 6 cut(s) 253, 273, 493, 496, 499, 930
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 564
Fsp4HI GCNGC 6 cut(s) 253, 273, 493, 496, 499, 930
FspBI CTAG 3 cut(s) 474, 689, 890
GlaI GCGC 3 cut(s) 271, 738, 876
GluI GCNGC 6 cut(s) 253, 273, 493, 496, 499, 930
GsaI CCCAGC 1 cut(s) 779
HaeII RGCGCY 2 cut(s) 273, 740
HaeIII GGCC 4 cut(s) 365, 377, 426, 750
HapII CCGG 9 cut(s) 47, 114, 122, 367, 374, 435, 712, 734, 751
HgaI GACGC 3 cut(s) 101, 752, 881
HhaI GCGC 3 cut(s) 272, 739, 877
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 4 cut(s) 397, 568, 601, 682
Hin6I GCGC 3 cut(s) 270, 737, 875
HinP1I GCGC 3 cut(s) 270, 737, 875
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HinfI GANTC 3 cut(s) 174, 278, 798
HpaII CCGG 9 cut(s) 47, 114, 122, 367, 374, 435, 712, 734, 751
HphI GGTGA 2 cut(s) 301, 476
Hpy166II GTNNAC 6 cut(s) 40, 52, 289, 820, 871, 916
Hpy188I TCNGA 7 cut(s) 154, 182, 455, 628, 797, 803, 813
Hpy188III TCNNGA 2 cut(s) 415, 613
Hpy8I GTNNAC 6 cut(s) 40, 52, 289, 820, 871, 916
Hpy99I CGWCG 4 cut(s) 56, 95, 389, 746
HpyAV CCTTC 2 cut(s) 313, 918
HpyCH4III ACNGT 3 cut(s) 214, 350, 464
HpyCH4IV ACGT 2 cut(s) 164, 865
HpyCH4V TGCA 1 cut(s) 568
HpyF10VI GCNNNNNNNGC 7 cut(s) 75, 362, 398, 498, 504, 533, 743
HpyF3I CTNAG 3 cut(s) 266, 855, 933
HpySE526I ACGT 2 cut(s) 164, 865
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 4 cut(s) 397, 568, 601, 682
HspAI GCGC 3 cut(s) 270, 737, 875
Kzo9I GATC 7 cut(s) 13, 450, 579, 609, 765, 792, 808
LmnI GCTCC 5 cut(s) 264, 305, 375, 559, 628
Lsp1109I GCAGC 3 cut(s) 479, 485, 916
LweI GCATC 1 cut(s) 379
MaeI CTAG 3 cut(s) 474, 689, 890
MaeII ACGT 2 cut(s) 164, 865
MaeIII GTNAC 2 cut(s) 464, 838
MalI GATC 7 cut(s) 15, 452, 581, 611, 767, 794, 810
MboI GATC 7 cut(s) 13, 450, 579, 609, 765, 792, 808
MboII GAAGA 1 cut(s) 169
MflI RGATCY 3 cut(s) 450, 609, 808
MhlI GDGCHC 2 cut(s) 564, 672
MluCI AATT 3 cut(s) 8, 343, 635
MlyI GAGTC 1 cut(s) 272
MmeI TCCRAC 5 cut(s) 205, 225, 318, 606, 793
MseI TTAA 1 cut(s) 951
MspI CCGG 9 cut(s) 47, 114, 122, 367, 374, 435, 712, 734, 751
MspR9I CCNGG 7 cut(s) 47, 114, 122, 367, 374, 435, 436
MvnI CGCG 2 cut(s) 875, 877
MwoI GCNNNNNNNGC 7 cut(s) 75, 362, 398, 498, 504, 533, 743
NciI CCSGG 7 cut(s) 47, 114, 122, 367, 374, 435, 436
NcoI CCATGG 1 cut(s) 393
NdeII GATC 7 cut(s) 13, 450, 579, 609, 765, 792, 808
NlaIII CATG 4 cut(s) 397, 568, 601, 682
NlaIV GGNNCC 8 cut(s) 260, 371, 452, 561, 611, 686, 716, 896
NmeAIII GCCGAG 1 cut(s) 503
NmuCI GTSAC 2 cut(s) 464, 838
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PfeI GAWTC 2 cut(s) 174, 798
PflFI GACNNNGTC 2 cut(s) 122, 622
PflMI CCANNNNNTGG 3 cut(s) 165, 408, 775
PkrI GCNGC 6 cut(s) 254, 274, 494, 497, 500, 931
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
Ppu21I YACGTR 1 cut(s) 866
PpuMI RGGWCCY 1 cut(s) 685
Psp5II RGGWCCY 1 cut(s) 685
PspEI GGTNACC 1 cut(s) 464
PspFI CCCAGC 1 cut(s) 775
PspN4I GGNNCC 8 cut(s) 260, 371, 452, 561, 611, 686, 716, 896
PspPI GGNCC 7 cut(s) 364, 376, 424, 618, 646, 685, 709
PspPPI RGGWCCY 1 cut(s) 685
PsuI RGATCY 3 cut(s) 450, 609, 808
PsyI GACNNNGTC 2 cut(s) 122, 622
RsaI GTAC 1 cut(s) 488
RsaNI GTAC 1 cut(s) 487
Rsr2I CGGWCCG 1 cut(s) 709
RsrII CGGWCCG 1 cut(s) 709
SalI GTCGAC 1 cut(s) 50
SaqAI TTAA 1 cut(s) 951
SatI GCNGC 6 cut(s) 253, 273, 493, 496, 499, 930
Sau3AI GATC 7 cut(s) 13, 450, 579, 609, 765, 792, 808
Sau96I GGNCC 7 cut(s) 364, 376, 424, 618, 646, 685, 709
SchI GAGTC 1 cut(s) 272
ScrFI CCNGG 7 cut(s) 47, 114, 122, 367, 374, 435, 436
SduI GDGCHC 2 cut(s) 564, 672
SfaNI GCATC 1 cut(s) 379
SinI GGWCC 4 cut(s) 618, 646, 685, 709
SmaI CCCGGG 1 cut(s) 436
Sse9I AATT 3 cut(s) 8, 343, 635
SspI AATATT 1 cut(s) 904
SspMI CTAG 3 cut(s) 474, 689, 890
StyD4I CCNGG 7 cut(s) 45, 112, 120, 365, 372, 433, 434
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 3 cut(s) 214, 350, 464
TaiI ACGT 2 cut(s) 167, 868
TaqI TCGA 4 cut(s) 51, 429, 672, 741
TasI AATT 3 cut(s) 8, 343, 635
TauI GCSGC 3 cut(s) 255, 275, 498
TfiI GAWTC 2 cut(s) 174, 798
Tru1I TTAA 1 cut(s) 951
Tru9I TTAA 1 cut(s) 951
TscAI CASTG 3 cut(s) 413, 452, 699
TseFI GTSAC 2 cut(s) 464, 838
TseI GCWGC 3 cut(s) 492, 498, 929
Tsp45I GTSAC 2 cut(s) 464, 838
TspDTI ATGAA 4 cut(s) 295, 555, 560, 602
TspGWI ACGGA 4 cut(s) 274, 328, 659, 722
TspMI CCCGGG 1 cut(s) 434
TspRI CASTG 3 cut(s) 413, 452, 699
Tth111I GACNNNGTC 2 cut(s) 122, 622
Van91I CCANNNNNTGG 3 cut(s) 165, 408, 775
VpaK11BI GGWCC 4 cut(s) 618, 646, 685, 709
XapI RAATTY 2 cut(s) 8, 635
XmaI CCCGGG 1 cut(s) 434
XmiI GTMKAC 3 cut(s) 51, 870, 915
XspI CTAG 3 cut(s) 474, 689, 890
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.