Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0007610

Physical Location & Seq

Genomic Coordinates Forward (+)

14614484 .. 14622607

Gene Structure 8124 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g17100.t5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3156 bp

ATGGATGAAAAGCCATTGCCGGCATGGACATGGTCAGTTGATCAGTGTCTGAAAAAGCATCATGTAAAACTAGAGCAGGGTCTGAGCACTTTTGAGGTTGAGAAGCGGCGTGAAAGGTACGGTTGGAATGAGCTTACCAAAGAGAAAGGGAAGCCTTTATGGCGGCTTGTATTAGAGCAGTTTGATGACACACTTGTGAAGATACTTCTAGCTGCAGCCTTCATCTCGTTCGCTTTAGCTTTCATGCATGGAAGTGAGTCAGGGGAGTCAGGGTTTGAGGCCTATGTGGAACCATTTGTGATAGTCTTGATTCTGGTGCTTAATGCTATTGTTGGTGTTTGGCAAGAGAGCAATGCTGAAAAAGCACTTGAAGCTCTTAAACAGATGCAAAGTGAATCTGCAAAGGTCCTAAGGGACATGTATTTGGTGCCAGATTTGCCTGCAAGAGAACTTGTCCCTGGCGATATTGTGGAGTTGCGAGTTGGGGACAAAGTCCCTGCTGATATGAGAGTCGCAGTTTTGAAAACCTCAACTTTGCGAGTTGAGCAGAGCTCATTGACCGGAGAGGCAATGCCTGTTTTGAAGAGCACTGATCCTATATTCATTGATGACTGTGAGCTGCAAGCTAAAGAAAGTATGGTTTTTGCAGGCACAACAGTTGTAAATGGTAGCTGTCTTTGTATCGTTGTTAGCACTGGGATGAACACTGAGATTGGCAAAATACAAAAGCAAATACATGAGGCTTCTTTGGAAGAAGATGACACCCCTTTGAAGAAGAAGCTTGATGAGTTTGGGAGCAAGTTTACTACAGGAATTGGGTTCGCTTGCCTCATTGTTTGGGTTATGAACTACAAAAACTTCATTTCATGGGAGATGAAAGATGGATGGCCAGTAAATGTGCGGTTTTCTTTTGAGAGGTGCACATACTATTTTAAGATAGCTGTTGCACTTGCAGTAGCTGCAATCCCAGAAGGTCTTCCTGCTGTTATTACAACTTGCTTAGCTCTTGGGACAAGGAAGATGGCACAAAAAAATGCAATTGTGAGGAAGCTTCCGAGTGTAGAAACCTTAGGATGTACAAGTGTGATTTGCTCAGATAAAACTGGGACTTTGACAACAAATCAGATGTCTGTGACAGAATTCTTCACTTTGGGTGGGAAAACCACAGCTTCTCGAATGATTCATGTTCAAGGCACAACTTATGATCCCAAGGATGGGGGAATTGTTGATTGGACTTGCTTCAATATGGATGCAAATATGCAAGCCATGGCAGAAATATGTGCTGTATGCAATGATGCTGGGATTTATTTTGATGGTCAGCTCTTTCGAGCTACAGGTTTACCAACTGAGGCAGCTCTTAAAGTTTTGGTTGAAAAGATGGGAGTTCCAGACATTAAGGCAAGGAACAAAGTTCGTGACTCACAGCTTGCTGCAAGCTACTTAATTGACTCCACCTCAGTGAAATTAGGCTGTTGTGAGTGGTGGACAAAAAGATCGAAAAAGGTTGCTACACTAGAGTTTGACCGTGTCCGAAAATCAATGAGTGTTATTGCTCAGGGGCCAACTGGGCATAATAGACTTCTTGTTAAGGGTGCTGTTGAGAGTTTACTAGAACGAACTTCGCATGTACAACTTGCAGATGGATCCGTTGTTCCAATAGATGAACCTTGTAAACAATCATTGCTAATGAGACTCTTGGAGATGAGTTCAAAGGGTTTAAGGTGCTTGGGTTTTGCATATAAAGATAACTTAGGAGAGCTCTCAGATTATTCTTCTGGGAGTCATCCAGCTAACAAGATGTTGCAGGATCCAGCCTGCTACTCCTCCATCGAAAGTGACTTAGTTTTTGTAGGAATTGTTGGCTTAAGAGACCCTCCTCGTGATGAAGTTGGCAAAGCAATCGAAGACTGTAAAGAAGCTGGAATTAGAGTTATGGTTATAACTGGAGATAATAAGTCTACAGCTGAAGCTATCTGTCAAGAAATCAAATTGTTTTCGAAAATTGAGGAGCTGAAAGGGAGAAGCTTTACAGGAAAAGAATTCATGGCTCTTTCACCATCACAACAAACGGAAATTTTATCAAAACCAGGAGGGAAGGTTTTCTCTCGTGCCGAGCCTAGACATAAGCAAGAAATAGTAAGGATGCTTAAAGAGATGGGGGAGATTGTAGCAATGACTGGAGATGGAGTGAATGATGCACCGGCACTTAAACTTGCTGACATTGGAATCGCCATGGGCATTACTGGAACGGAGGTAGCAAAAGAAGCTTCAGATATGGTTTTGGCTGATGACAATTTCAGTACCATTGTTTCAGCTGTTGCAGAGGGCCGTGCCATTTACACTAATATGAAAGCTTTTATCAGGTACATGATATCTTCAAATGTTGGAGAGGTAATATCCATATTCTTGACCGCGGCGCTGGGAATACCCGAGTGTATGATACCTGTGCAGCTTCTATGGGTAAATTTAGTTACAGATGGACCTCCTGCAACAGCTCTTGGCTTTAACCCGGCTGACATTCACATTATGAAGAAACCACCACGCAAAAGCAATGATGCTCTTATGAATTCTTGGGTTCTTTTCCGCTACTTGGTAATTGGTTCTTATGTGGGAATTGCAACTGTTGGAGTCTTCATCTTGTGGTACACTCAGGCTTCATTCATGGGTATCAATCTTGTGAGTGATGGACATACACTAGTTGGATTATCTCAGCTTCGAAATTGGGGAGTGTGTTCGTCGTGGTCAAATTTTACAGCAGCTCCATTCATGGTTAATGGGGGGAGGATGATCACTTTCACTGATCCCTGTGACTATTTCTCGGTTGGTAAAGTGAAGGCAATGACTTTGTCACTCTCTGTCCTGGTGGCAATTGAGATGTTCAACTCTCTTAATGCTCTTTCCGAAGACATTAGCTTGCTAAAAATGCCTCCTTGGAAGAACCCTTGGCTTTTGGTTGCCATGTCAGCCTCATTCGGACTCCATTGCCTCATTCTCTACATTCCATTTTTAGCGGATGTGTTTGGTGTTGTCCCGTTGAATCTGAACGAATGGCTTCTCGTAGTTATGATTTCGGCACCAGTGATTCTTATCGATGAGGTTCTCAAGTTAGTAGGAAGGAGTAGACGATGGAGAGCAAAGAAGGAGAAGACAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0034220 GO:0042175 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1052

Amino Acids

115.61

Weight (kDa)

6.09

Isoelectric Point (pI)

37.03

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	9 - 76	7.8e-21	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	129 - 245	1.3e-25	P-type ATPase actuator domain
Cation_ATPase	PF13246	420 - 629	3e-40	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	617 - 740	4.3e-14	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	811 - 1037	2e-46	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007610)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00900 AT4G00900
fragaria_vesca	FvH4_7g17100 FvH4_7g17100 FvH4_7g17100 FvH4_7g17100
malus_domestica	MD07G1154700.v1.1
prunus_persica	Prupe.2G193300_v2.0.a1 Prupe.2G193300_v2.0.a1 Prupe.2G193300_v2.0.a1 Prupe.2G193300_v2.0.a1 Prupe.2G193300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0360511
rosa_laevigata	RLG00000027800
rosa_multiflora	Rmu_ssc0000337.1_g000006
rosa_roxburghii	Rroxscaffold_4G00296030
rosa_rugosa	Rorug01G0279000 Rorug01G0279100 Rorug01G0279200 Rorug01G0279300 Rorug01G0279400
rosa_samantha	Rh1AG291600 Rh1CG274300
rosa_wichuraiana	Rw1G025830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1940
AccB1I	GGYRCC	2 cut(s)	427, 3073
AccI	GTMKAC	2 cut(s)	1958, 3121
AccII	CGCG	1 cut(s)	2414
AciI	CCGC	7 cut(s)	106, 163, 901, 2412, 2414, 2584, 3011
AclWI	GGATC	7 cut(s)	587, 1199, 1638, 1651, 1802, 1815, 2795
AcoI	YGGCCR	1 cut(s)	887
AcsI	RAATTY	6 cut(s)	1139, 2039, 2073, 2464, 2566, 2746
AcuI	CTGAAG	2 cut(s)	1986, 2253
AfaI	GTAC	6 cut(s)	119, 1078, 1629, 2302, 2366, 2645
AfiI	CCNNNNNNNGG	3 cut(s)	1214, 1215, 2590
AflII	CTTAAG	1 cut(s)	1864
AflIII	ACRYGT	1 cut(s)	417
AhlI	ACTAGT	1 cut(s)	2695
AjnI	CCWGG	3 cut(s)	457, 2086, 2859
AjuI	GAANNNNNNNTTGG	2 cut(s)	1213, 1245
AleI	CACNNNNGTG	2 cut(s)	194, 1457
AloI	GAACNNNNNNTCC	2 cut(s)	1635, 1667
Alw21I	GWGCWC	5 cut(s)	89, 554, 590, 923, 1761
Alw26I	GTCTC	2 cut(s)	1684, 1863
Alw44I	GTGCAC	1 cut(s)	919
AlwI	GGATC	7 cut(s)	587, 1199, 1638, 1651, 1802, 1815, 2795
AlwNI	CAGNNNCTG	3 cut(s)	49, 82, 959
Ama87I	CYCGRG	1 cut(s)	2429
AoxI	GGCC	4 cut(s)	279, 887, 1559, 2326
ApaLI	GTGCAC	1 cut(s)	919
ApeKI	GCWGC	8 cut(s)	212, 215, 619, 959, 1352, 1430, 2449, 2756
ApoI	RAATTY	6 cut(s)	1139, 2039, 2073, 2464, 2566, 2746
ArsI	GACNNNNNNTTYG	4 cut(s)	1112, 1144, 1829, 1861
Asp700I	GAANNNNTTC	3 cut(s)	975, 2099, 3051
AspLEI	GCGC	1 cut(s)	2419
AspS9I	GGNCC	4 cut(s)	406, 1559, 2326, 2480
AsuC2I	CCSGG	1 cut(s)	2510
AsuHPI	GGTGA	1 cut(s)	2046
AsuII	TTCGAA	2 cut(s)	1997, 2716
AvaI	CYCGRG	1 cut(s)	2429
AvaII	GGWCC	2 cut(s)	406, 2480
AxyI	CCTNAGG	2 cut(s)	410, 1069
BaeGI	GKGCMC	1 cut(s)	923
BalI	TGGCCA	1 cut(s)	889
BamHI	GGATCC	2 cut(s)	1643, 1807
BanI	GGYRCC	2 cut(s)	427, 3073
BanII	GRGCYC	2 cut(s)	554, 1761
BauI	CACGAG	2 cut(s)	1878, 2106
BbsI	GAAGAC	5 cut(s)	968, 1911, 2623, 2910, 3152
Bbv12I	GWGCWC	5 cut(s)	89, 554, 590, 923, 1761
BbvI	GCAGC	8 cut(s)	199, 227, 606, 946, 1364, 1417, 2461, 2768
BceAI	ACGGC	1 cut(s)	2313
BcgI	CGANNNNNNTGC	2 cut(s)	1882, 1916
BciT130I	CCWGG	3 cut(s)	459, 2088, 2861
BclI	TGATCA	2 cut(s)	40, 2787
BcnI	CCSGG	1 cut(s)	2510
BcoDI	GTCTC	2 cut(s)	1684, 1863
BcuI	ACTAGT	1 cut(s)	2695
BfaI	CTAG	6 cut(s)	71, 209, 1514, 1610, 2118, 2696
BfmI	CTRYAG	4 cut(s)	213, 807, 1332, 1959
BfoI	RGCGCY	1 cut(s)	2420
BfrI	CTTAAG	1 cut(s)	1864
BglI	GCCNNNNNGGC	2 cut(s)	160, 1567
BlpI	GCTNAGC	1 cut(s)	1000
Bme1390I	CCNGG	4 cut(s)	459, 2088, 2510, 2861
Bme18I	GGWCC	2 cut(s)	406, 2480
BmeT110I	CYCGRG	1 cut(s)	2429
BmgT120I	GGNCC	4 cut(s)	406, 1559, 2326, 2480
BmiI	GGNNCC	6 cut(s)	291, 429, 1560, 1645, 1809, 3075
BmrFI	CCNGG	4 cut(s)	459, 2088, 2510, 2861
BmrI	ACTGGG	3 cut(s)	705, 1113, 1575
BmsI	GCATC	7 cut(s)	67, 375, 1240, 1285, 2133, 2185, 2545
BmuI	ACTGGG	3 cut(s)	705, 1113, 1575
BpiI	GAAGAC	5 cut(s)	968, 1911, 2623, 2910, 3152
BplI	GAGNNNNNCTC	2 cut(s)	536, 568
BpmI	CTGGAG	2 cut(s)	1965, 2199
Bpu10I	CCTNAGC	1 cut(s)	1554
Bpu1102I	GCTNAGC	1 cut(s)	1000
Bpu14I	TTCGAA	2 cut(s)	1997, 2716
BpuEI	CTTGAG	1 cut(s)	3086
BpuMI	CCSGG	1 cut(s)	2510
Bsa29I	ATCGAT	1 cut(s)	3090
BsaBI	GATNNNNATC	1 cut(s)	3086
BsaI	GGTCTC	1 cut(s)	1863
BsaJI	CCNNGG	7 cut(s)	457, 1209, 1266, 2232, 2412, 2930, 2942
BsaWI	WCCGGW	1 cut(s)	560
BsaXI	ACNNNNNCTCC	8 cut(s)	257, 287, 2082, 2112, 2172, 2202, 2743, 2773
Bsc4I	CCNNNNNNNGG	3 cut(s)	1214, 1215, 2590
Bse118I	RCCGGY	2 cut(s)	19, 2200
Bse1I	ACTGG	8 cut(s)	700, 890, 1108, 1570, 1948, 2182, 2248, 3077
Bse21I	CCTNAGG	2 cut(s)	410, 1069
Bse3DI	GCAATG	9 cut(s)	14, 358, 576, 1297, 1679, 2178, 2557, 2844, 2980
Bse8I	GATNNNNATC	1 cut(s)	3086
BseBI	CCWGG	3 cut(s)	459, 2088, 2861
BseCI	ATCGAT	1 cut(s)	3090
BseDI	CCNNGG	7 cut(s)	457, 1209, 1266, 2232, 2412, 2930, 2942
BseJI	GATNNNNATC	1 cut(s)	3086
BseLI	CCNNNNNNNGG	3 cut(s)	1214, 1215, 2590
BseMI	GCAATG	9 cut(s)	14, 358, 576, 1297, 1679, 2178, 2557, 2844, 2980
BseMII	CTCAG	9 cut(s)	74, 699, 1107, 1338, 1470, 1568, 1776, 2663, 2723
BseNI	ACTGG	8 cut(s)	700, 890, 1108, 1570, 1948, 2182, 2248, 3077
BseRI	GAGGAG	3 cut(s)	1813, 1866, 2021
BseSI	GKGCMC	1 cut(s)	923
BseXI	GCAGC	8 cut(s)	199, 227, 606, 946, 1364, 1417, 2461, 2768
BseYI	CCCAGC	2 cut(s)	1298, 2419
BsgI	GTGCAG	1 cut(s)	2468
Bsh1236I	CGCG	1 cut(s)	2414
BshFI	GGCC	4 cut(s)	281, 889, 1561, 2328
BshNI	GGYRCC	2 cut(s)	427, 3073
BshVI	ATCGAT	1 cut(s)	3090
BsiHKAI	GWGCWC	5 cut(s)	89, 554, 590, 923, 1761
BsiHKCI	CYCGRG	1 cut(s)	2429
BsiSI	CCGG	4 cut(s)	20, 561, 2201, 2510
BslFI	GGGAC	7 cut(s)	428, 440, 479, 500, 1024, 1120, 3014
BslI	CCNNNNNNNGG	3 cut(s)	1214, 1215, 2590
BsmAI	GTCTC	2 cut(s)	1684, 1863
BsmFI	GGGAC	7 cut(s)	428, 440, 479, 500, 1024, 1120, 3014
BsnI	GGCC	4 cut(s)	281, 889, 1561, 2328
Bso31I	GGTCTC	1 cut(s)	1863
BsoBI	CYCGRG	1 cut(s)	2429
Bsp119I	TTCGAA	2 cut(s)	1997, 2716
Bsp1286I	GDGCHC	5 cut(s)	89, 554, 590, 923, 1761
Bsp1407I	TGTACA	2 cut(s)	1076, 1627
Bsp143I	GATC	8 cut(s)	40, 592, 1204, 1493, 1643, 1807, 2787, 2800
Bsp1720I	GCTNAGC	1 cut(s)	1000
Bsp19I	CCATGG	2 cut(s)	1266, 2232
BspACI	CCGC	7 cut(s)	106, 163, 901, 2412, 2414, 2584, 3011
BspANI	GGCC	4 cut(s)	281, 889, 1561, 2328
BspCNI	CTCAG	9 cut(s)	75, 700, 1106, 1339, 1469, 1567, 1775, 2662, 2722
BspDI	ATCGAT	1 cut(s)	3090
BspFNI	CGCG	1 cut(s)	2414
BspLI	GGNNCC	6 cut(s)	291, 429, 1560, 1645, 1809, 3075
BspMAI	CTGCAG	1 cut(s)	217
BspPI	GGATC	7 cut(s)	587, 1199, 1638, 1651, 1802, 1815, 2795
BspQI	GCTCTTC	1 cut(s)	578
BspT104I	TTCGAA	2 cut(s)	1997, 2716
BspT107I	GGYRCC	2 cut(s)	427, 3073
BspTI	CTTAAG	1 cut(s)	1864
BspTNI	GGTCTC	1 cut(s)	1863
BsrDI	GCAATG	9 cut(s)	14, 358, 576, 1297, 1679, 2178, 2557, 2844, 2980
BsrFI	RCCGGY	2 cut(s)	19, 2200
BsrGI	TGTACA	2 cut(s)	1076, 1627
BsrI	ACTGG	8 cut(s)	700, 890, 1108, 1570, 1948, 2182, 2248, 3077
BssAI	RCCGGY	2 cut(s)	19, 2200
BssECI	CCNNGG	7 cut(s)	457, 1209, 1266, 2232, 2412, 2930, 2942
BssMI	GATC	8 cut(s)	40, 592, 1204, 1493, 1643, 1807, 2787, 2800
BssSI	CACGAG	2 cut(s)	1878, 2106
BssT1I	CCWWGG	5 cut(s)	1209, 1266, 2232, 2930, 2942
Bst2BI	CACGAG	2 cut(s)	1878, 2106
Bst2UI	CCWGG	3 cut(s)	459, 2088, 2861
Bst4CI	ACNGT	6 cut(s)	122, 614, 658, 1526, 1910, 2623
Bst6I	CTCTTC	1 cut(s)	578
BstAFI	CTTAAG	1 cut(s)	1864
BstAPI	GCANNNNNTGC	1 cut(s)	959
BstAUI	TGTACA	2 cut(s)	1076, 1627
BstBI	TTCGAA	2 cut(s)	1997, 2716
BstDSI	CCRYGG	3 cut(s)	1266, 2232, 2412
BstFNI	CGCG	1 cut(s)	2414
BstH2I	RGCGCY	1 cut(s)	2420
BstHHI	GCGC	1 cut(s)	2419
BstKTI	GATC	8 cut(s)	43, 595, 1207, 1496, 1646, 1810, 2790, 2803
BstMAI	GTCTC	2 cut(s)	1684, 1863
BstMBI	GATC	8 cut(s)	40, 592, 1204, 1493, 1643, 1807, 2787, 2800
BstNI	CCWGG	3 cut(s)	459, 2088, 2861
BstNSI	RCATGY	2 cut(s)	421, 1628
BstSCI	CCNGG	4 cut(s)	457, 2086, 2508, 2859
BstSFI	CTRYAG	4 cut(s)	213, 807, 1332, 1959
BstSLI	GKGCMC	1 cut(s)	923
BstUI	CGCG	1 cut(s)	2414
BstV1I	GCAGC	8 cut(s)	199, 227, 606, 946, 1364, 1417, 2461, 2768
BstV2I	GAAGAC	5 cut(s)	968, 1911, 2623, 2910, 3152
BstX2I	RGATCY	2 cut(s)	1643, 1807
BstYI	RGATCY	2 cut(s)	1643, 1807
Bsu15I	ATCGAT	1 cut(s)	3090
Bsu36I	CCTNAGG	2 cut(s)	410, 1069
BsuRI	GGCC	4 cut(s)	281, 889, 1561, 2328
BsuTUI	ATCGAT	1 cut(s)	3090
BtgI	CCRYGG	3 cut(s)	1266, 2232, 2412
BtsIMutI	CAGTG	7 cut(s)	50, 588, 693, 705, 1464, 2796, 3084
CaiI	CAGNNNCTG	3 cut(s)	49, 82, 959
CfoI	GCGC	1 cut(s)	2419
Cfr10I	RCCGGY	2 cut(s)	19, 2200
Cfr13I	GGNCC	4 cut(s)	406, 1559, 2326, 2480
Cfr42I	CCGCGG	1 cut(s)	2415
ClaI	ATCGAT	1 cut(s)	3090
Csp6I	GTAC	6 cut(s)	118, 1077, 1628, 2301, 2365, 2644
CviQI	GTAC	6 cut(s)	118, 1077, 1628, 2301, 2365, 2644
DpnI	GATC	8 cut(s)	42, 594, 1206, 1495, 1645, 1809, 2789, 2802
DpnII	GATC	8 cut(s)	40, 592, 1204, 1493, 1643, 1807, 2787, 2800
EaeI	YGGCCR	1 cut(s)	887
Eam1104I	CTCTTC	1 cut(s)	578
EarI	CTCTTC	1 cut(s)	578
Ecl136II	GAGCTC	2 cut(s)	552, 1759
Eco130I	CCWWGG	5 cut(s)	1209, 1266, 2232, 2930, 2942
Eco147I	AGGCCT	1 cut(s)	281
Eco24I	GRGCYC	2 cut(s)	554, 1761
Eco31I	GGTCTC	1 cut(s)	1863
Eco32I	GATATC	1 cut(s)	2373
Eco47I	GGWCC	2 cut(s)	406, 2480
Eco53kI	GAGCTC	2 cut(s)	552, 1759
Eco57I	CTGAAG	2 cut(s)	1986, 2253
Eco81I	CCTNAGG	2 cut(s)	410, 1069
Eco88I	CYCGRG	1 cut(s)	2429
EcoICRI	GAGCTC	2 cut(s)	552, 1759
EcoO109I	RGGNCCY	1 cut(s)	406
EcoRI	GAATTC	3 cut(s)	1139, 2039, 2566
EcoRII	CCWGG	3 cut(s)	457, 2086, 2859
EcoRV	GATATC	1 cut(s)	2373
EcoT14I	CCWWGG	5 cut(s)	1209, 1266, 2232, 2930, 2942
EcoT22I	ATGCAT	1 cut(s)	249
EcoT38I	GRGCYC	2 cut(s)	554, 1761
ErhI	CCWWGG	5 cut(s)	1209, 1266, 2232, 2930, 2942
FaqI	GGGAC	7 cut(s)	428, 440, 479, 500, 1024, 1120, 3014
FbaI	TGATCA	2 cut(s)	40, 2787
FblI	GTMKAC	2 cut(s)	1958, 3121
FriOI	GRGCYC	2 cut(s)	554, 1761
FspBI	CTAG	6 cut(s)	71, 209, 1514, 1610, 2118, 2696
GlaI	GCGC	1 cut(s)	2418
GsaI	CCCAGC	2 cut(s)	1302, 2423
GsuI	CTGGAG	2 cut(s)	1965, 2199
HaeII	RGCGCY	1 cut(s)	2420
HaeIII	GGCC	4 cut(s)	281, 889, 1561, 2328
HapII	CCGG	4 cut(s)	20, 561, 2201, 2510
HhaI	GCGC	1 cut(s)	2419
Hin6I	GCGC	1 cut(s)	2417
HinP1I	GCGC	1 cut(s)	2417
HindIII	AAGCTT	5 cut(s)	779, 1049, 2023, 2265, 2352
HpaII	CCGG	4 cut(s)	20, 561, 2201, 2510
HphI	GGTGA	1 cut(s)	2046
Hpy166II	GTNNAC	9 cut(s)	804, 921, 1340, 1485, 1607, 1673, 1959, 2646, 3122
Hpy188III	TCNNGA	7 cut(s)	307, 1173, 1388, 1415, 1880, 1979, 2407
Hpy8I	GTNNAC	9 cut(s)	804, 921, 1340, 1485, 1607, 1673, 1959, 2646, 3122
Hpy99I	CGWCG	1 cut(s)	2740
HpyAV	CCTTC	6 cut(s)	229, 965, 2089, 2827, 3108, 3133
HpyCH4III	ACNGT	6 cut(s)	122, 614, 658, 1526, 1910, 2623
HspAI	GCGC	1 cut(s)	2417
KroI	GCCGGC	1 cut(s)	19
KroNI	GCCGGC	1 cut(s)	21
Ksp22I	TGATCA	2 cut(s)	40, 2787
KspI	CCGCGG	1 cut(s)	2415
Kzo9I	GATC	8 cut(s)	40, 592, 1204, 1493, 1643, 1807, 2787, 2800
LguI	GCTCTTC	1 cut(s)	578
LmnI	GCTCC	3 cut(s)	795, 2008, 2764
Lsp1109I	GCAGC	8 cut(s)	199, 227, 606, 946, 1364, 1417, 2461, 2768
LweI	GCATC	7 cut(s)	67, 375, 1240, 1285, 2133, 2185, 2545
MaeI	CTAG	6 cut(s)	71, 209, 1514, 1610, 2118, 2696
MaeIII	GTNAC	6 cut(s)	1132, 1415, 1835, 2470, 2807, 2847
MalI	GATC	8 cut(s)	42, 594, 1206, 1495, 1645, 1809, 2789, 2802
MboI	GATC	8 cut(s)	40, 592, 1204, 1493, 1643, 1807, 2787, 2800
MfeI	CAATTG	2 cut(s)	1038, 2868
MflI	RGATCY	2 cut(s)	1643, 1807
MhlI	GDGCHC	5 cut(s)	89, 554, 590, 923, 1761
MlsI	TGGCCA	1 cut(s)	889
MluNI	TGGCCA	1 cut(s)	889
MlyI	GAGTC	9 cut(s)	266, 275, 519, 1412, 1442, 1686, 1789, 2637, 2970
MmeI	TCCRAC	4 cut(s)	104, 2365, 2605, 2680
Mox20I	TGGCCA	1 cut(s)	889
Mph1103I	ATGCAT	1 cut(s)	249
MroNI	GCCGGC	1 cut(s)	19
MroXI	GAANNNNTTC	3 cut(s)	975, 2099, 3051
MscI	TGGCCA	1 cut(s)	889
MslI	CAYNNNNRTG	6 cut(s)	28, 194, 252, 698, 1457, 2345
Msp20I	TGGCCA	1 cut(s)	889
MspA1I	CMGCKG	3 cut(s)	1964, 2315, 2414
MspCI	CTTAAG	1 cut(s)	1864
MspI	CCGG	4 cut(s)	20, 561, 2201, 2510
MspR9I	CCNGG	4 cut(s)	459, 2088, 2510, 2861
MunI	CAATTG	2 cut(s)	1038, 2868
MvaI	CCWGG	3 cut(s)	459, 2088, 2861
MvnI	CGCG	1 cut(s)	2414
NaeI	GCCGGC	1 cut(s)	21
NciI	CCSGG	1 cut(s)	2510
NcoI	CCATGG	2 cut(s)	1266, 2232
NdeII	GATC	8 cut(s)	40, 592, 1204, 1493, 1643, 1807, 2787, 2800
NgoMIV	GCCGGC	1 cut(s)	19
NlaIV	GGNNCC	6 cut(s)	291, 429, 1560, 1645, 1809, 3075
NmeAIII	GCCGAG	1 cut(s)	2137
NmuCI	GTSAC	5 cut(s)	1132, 1415, 1835, 2807, 2847
NsiI	ATGCAT	1 cut(s)	249
NspI	RCATGY	2 cut(s)	421, 1628
NspV	TTCGAA	2 cut(s)	1997, 2716
OliI	CACNNNNGTG	2 cut(s)	194, 1457
PceI	AGGCCT	1 cut(s)	281
PciI	ACATGT	1 cut(s)	417
PciSI	GCTCTTC	1 cut(s)	578
PdiI	GCCGGC	1 cut(s)	21
PdmI	GAANNNNTTC	3 cut(s)	975, 2099, 3051
PfeI	GAWTC	6 cut(s)	310, 395, 1180, 2226, 3037, 3082
PflFI	GACNNNGTC	4 cut(s)	31, 491, 1526, 2845
PleI	GAGTC	9 cut(s)	265, 274, 518, 1412, 1442, 1686, 1788, 2636, 2970
PpsI	GAGTC	9 cut(s)	265, 274, 518, 1412, 1442, 1686, 1788, 2636, 2970
PpuMI	RGGWCCY	1 cut(s)	406
PscI	ACATGT	1 cut(s)	417
PsiI	TTATAA	1 cut(s)	1940
Psp124BI	GAGCTC	2 cut(s)	554, 1761
Psp5II	RGGWCCY	1 cut(s)	406
Psp6I	CCWGG	3 cut(s)	457, 2086, 2859
PspFI	CCCAGC	2 cut(s)	1298, 2419
PspGI	CCWGG	3 cut(s)	457, 2086, 2859
PspN4I	GGNNCC	6 cut(s)	291, 429, 1560, 1645, 1809, 3075
PspPI	GGNCC	4 cut(s)	406, 1559, 2326, 2480
PspPPI	RGGWCCY	1 cut(s)	406
PstI	CTGCAG	1 cut(s)	217
PstNI	CAGNNNCTG	3 cut(s)	49, 82, 959
PsuI	RGATCY	2 cut(s)	1643, 1807
PsyI	GACNNNGTC	4 cut(s)	31, 491, 1526, 2845
PvuII	CAGCTG	2 cut(s)	1964, 2315
RsaI	GTAC	6 cut(s)	119, 1078, 1629, 2302, 2366, 2645
RsaNI	GTAC	6 cut(s)	118, 1077, 1628, 2301, 2365, 2644
RseI	CAYNNNNRTG	6 cut(s)	28, 194, 252, 698, 1457, 2345
SacI	GAGCTC	2 cut(s)	554, 1761
SacII	CCGCGG	1 cut(s)	2415
SapI	GCTCTTC	1 cut(s)	578
Sau3AI	GATC	8 cut(s)	40, 592, 1204, 1493, 1643, 1807, 2787, 2800
Sau96I	GGNCC	4 cut(s)	406, 1559, 2326, 2480
SchI	GAGTC	9 cut(s)	266, 275, 519, 1412, 1442, 1686, 1789, 2637, 2970
ScrFI	CCNGG	4 cut(s)	459, 2088, 2510, 2861
SduI	GDGCHC	5 cut(s)	89, 554, 590, 923, 1761
SfaNI	GCATC	7 cut(s)	67, 375, 1240, 1285, 2133, 2185, 2545
SfcI	CTRYAG	4 cut(s)	213, 807, 1332, 1959
Sfr303I	CCGCGG	1 cut(s)	2415
SfuI	TTCGAA	2 cut(s)	1997, 2716
SgrBI	CCGCGG	1 cut(s)	2415
SinI	GGWCC	2 cut(s)	406, 2480
SmiMI	CAYNNNNRTG	6 cut(s)	28, 194, 252, 698, 1457, 2345
SmlI	CTYRAG	2 cut(s)	1864, 3101
SmoI	CTYRAG	2 cut(s)	1864, 3101
SpeI	ACTAGT	1 cut(s)	2695
SseBI	AGGCCT	1 cut(s)	281
SsiI	CCGC	7 cut(s)	106, 163, 901, 2412, 2414, 2584, 3011
SspMI	CTAG	6 cut(s)	71, 209, 1514, 1610, 2118, 2696
SstI	GAGCTC	2 cut(s)	554, 1761
StuI	AGGCCT	1 cut(s)	281
StyD4I	CCNGG	4 cut(s)	457, 2086, 2508, 2859
StyI	CCWWGG	5 cut(s)	1209, 1266, 2232, 2930, 2942
TaaI	ACNGT	6 cut(s)	122, 614, 658, 1526, 1910, 2623
TaqI	TCGA	8 cut(s)	1174, 1327, 1496, 1830, 1902, 1997, 2716, 3090
TatI	WGTACW	2 cut(s)	1076, 1627
TauI	GCSGC	3 cut(s)	109, 166, 2417
TfiI	GAWTC	6 cut(s)	310, 395, 1180, 2226, 3037, 3082
TscAI	CASTG	7 cut(s)	50, 595, 700, 712, 1464, 2803, 3084
TseFI	GTSAC	5 cut(s)	1132, 1415, 1835, 2807, 2847
TseI	GCWGC	8 cut(s)	212, 215, 619, 959, 1352, 1430, 2449, 2756
Tsp45I	GTSAC	5 cut(s)	1132, 1415, 1835, 2807, 2847
TspGWI	ACGGA	3 cut(s)	1636, 2084, 2264
TspRI	CASTG	7 cut(s)	50, 595, 700, 712, 1464, 2803, 3084
Tth111I	GACNNNGTC	4 cut(s)	31, 491, 1526, 2845
Vha464I	CTTAAG	1 cut(s)	1864
VneI	GTGCAC	1 cut(s)	919
VpaK11BI	GGWCC	2 cut(s)	406, 2480
XapI	RAATTY	6 cut(s)	1139, 2039, 2073, 2464, 2566, 2746
XceI	RCATGY	2 cut(s)	421, 1628
XcmI	CCANNNNNNNNNTGG	1 cut(s)	21
XmiI	GTMKAC	2 cut(s)	1958, 3121
XmnI	GAANNNNTTC	3 cut(s)	975, 2099, 3051
XspI	CTAG	6 cut(s)	71, 209, 1514, 1610, 2118, 2696
Zsp2I	ATGCAT	1 cut(s)	249

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.