Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007610

Physical Location & Seq

Genomic Coordinates Forward (+)

22427641 .. 22432571

Gene Structure 4931 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1154700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3153 bp

ATGGAAGAAAAGCCGTTCCCGGCATGGTCATGGTCAATTGAGCAGTGCTTGAAGGAGTTACATGTGAAGCTAGATAAGGGTCTGAGCACTTATGAGGTTGAGAAGCGACGCGAAAGGCATGGCTGGAATGAGCTTGCCAAAGAGAAGGGGAAGCCGTTATGGCGGCTTGTACTCGAGCAGTTCGACGACACATTAGTGAAAATACTTCTGGTTGCAGCCTTCATATCTTTTGTTTTGGCTTTCATGGGTGGAGGTGAGTCAGGGGACTCCGGTTTTGAAGCCTATGTGGAACCGTTTGTGATAGTTTTGATTCTAGTCCTCAATGCCATTGTTGGAGTTTGGCAAGAGAGTAATGCTGAAAAAGCGCTCGAAGCACTTAAGCAGATGCAGAGTGAGTCCGGTAAGGTTTTGAGAGACGGGTATTTGGTGCCGGATTTACCTGCAAGGGAACTTGTTCCGGGTGACATTGTTGAGTTGCGCGTTGGAGACAAAGTTCCTGCTGACATGAGAGTGGTGGTTTTAAAGACTTCGACTTTGCGAGTTGAGCAGAGTTCATTGACTGGAGAGGCCATGCCGGTGTTGAAAAACACTGATCCTATATTCATGGATGACTGTGACTTGCAGGCTAAAGAGAATATGGTTTTTTCGGGCACAACAGTTGTCAACGGCAGCTGTCTTTGTGTTGTGATTAGCACTGGCATGAACACTGAGATTGGGAAAATCCAGAAGCAAATACATGAGGCTTCTTTGGAAGAAGATGACACCCCTTTGAAGAAGAAGCTGGACGAATTTGGAAGCCGGTTCACGACTGCAATTGGTTTTGTTTGCCTCATTGTTTGGGTTATGAACTACAAAAACTTTCTCTCATGGGATCTCGTTGATGGATGGCCAACGAATGTTAGGTTTTCGTTTGAGAGATGCACGTACTATTTCAAGATAGCTGTTGCACTCGCTGTGGCTGCAATCCCAGAAGGTCTTCCTGCTGTTATCACAACTTGCTTAGCTCTTGGGACAAGGAAAATGGCACAAAAGAATGCGATTGTGAGGAAGCTTACGAGCGTAGAAACCTTAGGATGCACAACTGTAATTTGTTCGGATAAAACGGGGACTTTGACAACAAACCAGATGTCTGTGACAGAGTTCTTCACTTTGGGTGGAAAAACCACTGCTTCTCGAACGATTCGTGTTGAAGGCACAACTTATGATCCCAAGGATGGCGGAATTGTTGATTGGAGTTGCTTTAACATGGATGTTAACATGCAGGCCATGGCAGAAATATGTGCTGTTTGCAATGATGCTGGCATTTATTTTGATGGCCAGCTCTTCAGATCTACGGGTTTGCCTACTGAGGCAGCTCTCAAGGTTTTGGTCGAAAAGATGGGAGTTCCAGACGCCAAAGCGAGGAACAAAATTCGTGACATGCAGCTCGCTGCAAGCTACTTAATCGACAGCACGGCAGCGAAATTAGGGTGTTGCGAGTGGTGGACAAAAAGATCGAAAAGGGTTGCCACGCTAGAATTTGATCGTGTCCGAAAGTCTATGAGTGTTATTGTCCGGGAGCCAACTGGGCGTAATCGACTACTTGTCAAGGGTGCCGTTGAGAGCTTACTGGAGCGCGCTTTCCATGTGCAACTTGCTGATGGATCCCTCGTTCCAATCGACGAAACTTGTAAACAGCTATTGCTCTCGAGACTCCAAGAGATGAGCTCAAAGGGATTGCGGTGCTTGGGATTTGCATACAAGGAGGAGTTGGGAGAGTTCTCAGATTATTCTTCCGCAAGTCATCCTGCTCACAAGAAGTTGCTTGATCCGGCCTGCTACTCCTCCATCGAAAGTGACTTAATTTTCGTAGGCATTGTTGGGTTAAGAGACCCTCCTCGCGATGAAGTTGGCAAAGCGATCGAAGATTGTAGAGAAGCTGGAATTAAAGTTATGGTGATAACTGGAGATAATAAGTCCACGGCTGAGGCAATCTGTCAAGAAATCAGATTGTTATCTAAAGACGAGGATATGAAAGGGAGAAGCTTTACAGGAAAAGAGTTCATGGTTCTTCCCCAGTCGCAACAAAAGGAAGTTTTATCGAAACCAGGAGGGAAGGTTTTCTCTCGTGCCGAGCCTAGGCATAAGCAAGAAATAGTAAGGATGCTGAAAGAGATGGGGGAGATTGTTGCAATGACCGGAGATGGAGTGAACGATGCACCAGCACTCAAACTCGCTGACATTGGAATCGCCATGGGAATTACTGGGACTGAGGTGGCGAAAGAAGCTTCGGATATGGTTTTGGCGGATGATAATTTCAGTACTATAGTTTCCGCTGTTGCAGAAGGCCGGGCCATTTACACTAATATGAAAGCTTTCATCAGGTACATGATATCTTCAAACGTCGGAGAAGTCATATCCATATTCTTGACCGCTGCGTTGGGAATACCAGAATGTATGATACCTGTGCAGCTTCTGTGGGTAAATCTGGTTACCGATGGACCGCCAGCAACAGCTCTCGGTTTTAACCCAGCTGACATTCATATTATGAAGAAACCACCTCGGAAAAGCAATGATCCTCTTATGAGTTCTTGGGTTCTCTTCCGCTACTTGGTAATTGGTTCCTACGTAGGCATCGCAACCGTTGGCATCTTCGTCTTGTGGTATACTCAGGCTTCCTTCATGGGCATCACTCTTGTGAGTGACGGACACACACTCGTTGAAGTGTCTCAACTTCGCAACTGGGGCGACTGCCCCTCATGGTCAAACTTCACAGCGGCTCCATTCACGGTTAGTGGGGGTCGGACAATCACTTTTTCCGACCCCTGTGACTATTTCTCTGTTGGGAAAGTGAAGGCAATGACTCTGTCCCTCTCAGTACTGGTGGCCATTGAGATGTTCAATTCCCTCAATGCTCTCTCTGAAGACATCAGCTTGGTGAAAATGCCACCTTGGAGGAACCCTTGGCTTCTGGTTGCCATGTCGGTCTCATTTGGGCTTCACTGCCTCATTCTCTACATCCCGTTTCTGGCAGACGTCTTCGGTGTTGTTCCGTTGAGCCTGAATGAGTGGCTCCTCGTAATTTTGATCTCCGCGCCGGTGATCCTTATCGACGAGGTTCTTAAATTAGTAGGAAGGAGGAGATGGAGAGCAAAGAAGGAGAAGACAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030176 GO:0031224 GO:0031227 GO:0031984 GO:0034220 GO:0042175 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0070588 GO:0070838 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0098827 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1051

Amino Acids

115.85

Weight (kDa)

5.79

Isoelectric Point (pI)

37.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	9 - 76	9.6e-21	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	129 - 245	2.1e-26	P-type ATPase actuator domain
Cation_ATPase	PF13246	420 - 629	3.5e-39	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	617 - 740	8.9e-13	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	811 - 1037	1e-47	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007610)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G00900 AT4G00900
fragaria_vesca	FvH4_7g17100 FvH4_7g17100 FvH4_7g17100 FvH4_7g17100
malus_domestica	MD07G1154700.v1.1
prunus_persica	Prupe.2G193300_v2.0.a1 Prupe.2G193300_v2.0.a1 Prupe.2G193300_v2.0.a1 Prupe.2G193300_v2.0.a1 Prupe.2G193300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0360511
rosa_laevigata	RLG00000027800
rosa_multiflora	Rmu_ssc0000337.1_g000006
rosa_roxburghii	Rroxscaffold_4G00296030
rosa_rugosa	Rorug01G0279000 Rorug01G0279100 Rorug01G0279200 Rorug01G0279300 Rorug01G0279400
rosa_samantha	Rh1AG291600 Rh1CG274300
rosa_wichuraiana	Rw1G025830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3018
Acc36I	ACCTGC	1 cut(s)	448
AccB1I	GGYRCC	2 cut(s)	427, 1592
AccI	GTMKAC	1 cut(s)	2645
AccII	CGCG	5 cut(s)	111, 480, 1617, 1881, 3074
AclWI	GGATC	8 cut(s)	587, 879, 1199, 1638, 1651, 1802, 2549, 3076
AcoI	YGGCCR	3 cut(s)	887, 1315, 2865
AcsI	RAATTY	3 cut(s)	788, 1410, 1517
AcuI	CTGAAG	2 cut(s)	1309, 2922
AcyI	GRCGYC	2 cut(s)	1392, 3015
AfaI	GTAC	5 cut(s)	171, 926, 2302, 2366, 2859
AfeI	AGCGCT	1 cut(s)	366
AfiI	CCNNNNNNNGG	5 cut(s)	1214, 1401, 2118, 2590, 3007
AflII	CTTAAG	1 cut(s)	377
AflIII	ACRYGT	1 cut(s)	61
AgsI	TTSAA	9 cut(s)	52, 278, 583, 772, 934, 1190, 2379, 2702, 2881
AhdI	GACNNNNNGTC	1 cut(s)	1583
AjnI	CCWGG	1 cut(s)	2086
AjuI	GAANNNNNNNTTGG	2 cut(s)	1213, 1245
AleI	CACNNNNGTG	2 cut(s)	194, 2675
AloI	GAACNNNNNNTCC	4 cut(s)	477, 509, 1635, 1667
Alw21I	GWGCWC	2 cut(s)	89, 1709
Alw26I	GTCTC	6 cut(s)	408, 480, 1684, 1863, 2712, 2971
AlwI	GGATC	8 cut(s)	587, 879, 1199, 1638, 1651, 1802, 2549, 3076
AlwNI	CAGNNNCTG	1 cut(s)	2455
Ama87I	CYCGRG	2 cut(s)	173, 1687
Aor51HI	AGCGCT	1 cut(s)	366
AoxI	GGCC	8 cut(s)	567, 887, 1263, 1315, 1812, 2326, 2331, 2865
ApeKI	GCWGC	9 cut(s)	215, 669, 959, 1352, 1423, 1430, 1457, 2414, 2449
ApoI	RAATTY	3 cut(s)	788, 1410, 1517
ArsI	GACNNNNNNTTYG	8 cut(s)	257, 289, 517, 549, 1112, 1144, 1829, 1861
Asp700I	GAANNNNTTC	4 cut(s)	453, 975, 2099, 2354
AspA2I	CCTAGG	1 cut(s)	2117
AspLEI	GCGC	5 cut(s)	367, 480, 1617, 1619, 3076
AspS9I	GGNCC	2 cut(s)	2331, 2480
AsuC2I	CCSGG	4 cut(s)	20, 459, 1556, 2330
AsuHPI	GGTGA	5 cut(s)	266, 473, 1948, 2929, 3091
AvaI	CYCGRG	2 cut(s)	173, 1687
AvaII	GGWCC	1 cut(s)	2480
AvrII	CCTAGG	1 cut(s)	2117
AxyI	CCTNAGG	1 cut(s)	1069
BaeGI	GKGCMC	1 cut(s)	653
BalI	TGGCCA	3 cut(s)	889, 1317, 2867
BamHI	GGATCC	1 cut(s)	1643
BanI	GGYRCC	2 cut(s)	427, 1592
BanII	GRGCYC	1 cut(s)	1709
BauI	CACGAG	1 cut(s)	2106
BbsI	GAAGAC	4 cut(s)	968, 2910, 3010, 3149
Bbv12I	GWGCWC	2 cut(s)	89, 1709
BbvCI	CCTCAGC	1 cut(s)	1965
BbvI	GCAGC	9 cut(s)	227, 681, 946, 1364, 1417, 1435, 1469, 2401, 2461
BceAI	ACGGC	5 cut(s)	139, 682, 1470, 1580, 1977
BcgI	CGANNNNNNTGC	2 cut(s)	1882, 1916
BciT130I	CCWGG	1 cut(s)	2088
BcnI	CCSGG	4 cut(s)	20, 459, 1556, 2330
BcoDI	GTCTC	6 cut(s)	408, 480, 1684, 1863, 2712, 2971
BfaI	CTAG	4 cut(s)	71, 314, 1514, 2118
BfmI	CTRYAG	1 cut(s)	2304
BfoI	RGCGCY	1 cut(s)	368
BfrI	CTTAAG	1 cut(s)	377
BfuAI	ACCTGC	1 cut(s)	448
BglI	GCCNNNNNGGC	2 cut(s)	160, 1567
BglII	AGATCT	1 cut(s)	1328
BlnI	CCTAGG	1 cut(s)	2117
BlpI	GCTNAGC	1 cut(s)	1000
BmcAI	AGTACT	2 cut(s)	2302, 2859
Bme1390I	CCNGG	5 cut(s)	20, 459, 1556, 2088, 2330
Bme18I	GGWCC	1 cut(s)	2480
BmeRI	GACNNNNNGTC	1 cut(s)	1583
BmeT110I	CYCGRG	2 cut(s)	173, 1687
BmgT120I	GGNCC	2 cut(s)	2331, 2480
BmiI	GGNNCC	9 cut(s)	291, 429, 1560, 1594, 1645, 2602, 2760, 2939, 3053
BmrFI	CCNGG	5 cut(s)	20, 459, 1556, 2088, 2330
BmrI	ACTGGG	4 cut(s)	1575, 2050, 2253, 2731
BmsI	GCATC	9 cut(s)	375, 908, 1064, 1285, 2133, 2185, 2622, 2637, 2676
BmuI	ACTGGG	4 cut(s)	1575, 2050, 2253, 2731
BpiI	GAAGAC	4 cut(s)	968, 2910, 3010, 3149
BplI	GAGNNNNNCTC	2 cut(s)	1691, 1723
BpmI	CTGGAG	3 cut(s)	582, 1631, 1965
Bpu10I	CCTNAGC	1 cut(s)	1965
Bpu1102I	GCTNAGC	1 cut(s)	1000
BpuEI	CTTGAG	1 cut(s)	1343
BpuMI	CCSGG	4 cut(s)	20, 459, 1556, 2330
BsaAI	YACGTR	2 cut(s)	924, 2608
BsaBI	GATNNNNATC	2 cut(s)	1993, 3086
BsaHI	GRCGYC	2 cut(s)	1392, 3015
BsaI	GGTCTC	2 cut(s)	1863, 2971
BsaJI	CCNNGG	8 cut(s)	1209, 1266, 1959, 2117, 2232, 2540, 2930, 2942
BsaWI	WCCGGW	3 cut(s)	269, 398, 2177
BsaXI	ACNNNNNCTCC	8 cut(s)	477, 507, 2082, 2112, 2172, 2202, 2743, 2773
Bsc4I	CCNNNNNNNGG	5 cut(s)	1214, 1401, 2118, 2590, 3007
Bse118I	RCCGGY	3 cut(s)	574, 798, 3076
Bse1I	ACTGG	9 cut(s)	565, 700, 1570, 1614, 1948, 2056, 2248, 2726, 2865
Bse21I	CCTNAGG	1 cut(s)	1069
Bse3DI	GCAATG	4 cut(s)	1297, 2178, 2557, 2844
Bse8I	GATNNNNATC	2 cut(s)	1993, 3086
BseBI	CCWGG	1 cut(s)	2088
BseDI	CCNNGG	8 cut(s)	1209, 1266, 1959, 2117, 2232, 2540, 2930, 2942
BseGI	GGATG	9 cut(s)	613, 890, 1079, 1219, 1255, 1783, 2148, 2293, 2997
BseJI	GATNNNNATC	2 cut(s)	1993, 3086
BseLI	CCNNNNNNNGG	5 cut(s)	1214, 1401, 2118, 2590, 3007
BseMI	GCAATG	4 cut(s)	1297, 2178, 2557, 2844
BseMII	CTCAG	8 cut(s)	74, 699, 1338, 1776, 1956, 2241, 2663, 2868
BseNI	ACTGG	9 cut(s)	565, 700, 1570, 1614, 1948, 2056, 2248, 2726, 2865
BsePI	GCGCGC	1 cut(s)	1615
BseRI	GAGGAG	5 cut(s)	1760, 1813, 1866, 3044, 3133
BseSI	GKGCMC	1 cut(s)	653
BseXI	GCAGC	9 cut(s)	227, 681, 946, 1364, 1417, 1435, 1469, 2401, 2461
BseYI	CCCAGC	1 cut(s)	2509
BsgI	GTGCAG	1 cut(s)	2468
Bsh1236I	CGCG	5 cut(s)	111, 480, 1617, 1881, 3074
Bsh1285I	CGRYCG	1 cut(s)	1902
BshFI	GGCC	8 cut(s)	569, 889, 1265, 1317, 1814, 2328, 2333, 2867
BshNI	GGYRCC	2 cut(s)	427, 1592
BsiEI	CGRYCG	1 cut(s)	1902
BsiHKAI	GWGCWC	2 cut(s)	89, 1709
BsiHKCI	CYCGRG	2 cut(s)	173, 1687
BslFI	GGGAC	5 cut(s)	278, 1024, 1120, 2260, 2833
BslI	CCNNNNNNNGG	5 cut(s)	1214, 1401, 2118, 2590, 3007
BsmAI	GTCTC	6 cut(s)	408, 480, 1684, 1863, 2712, 2971
BsmBI	CGTCTC	1 cut(s)	408
BsmFI	GGGAC	5 cut(s)	278, 1024, 1120, 2260, 2833
BsmI	GAATGC	1 cut(s)	1039
BsnI	GGCC	8 cut(s)	569, 889, 1265, 1317, 1814, 2328, 2333, 2867
Bso31I	GGTCTC	2 cut(s)	1863, 2971
BsoBI	CYCGRG	2 cut(s)	173, 1687
Bsp1286I	GDGCHC	3 cut(s)	89, 653, 1709
Bsp1720I	GCTNAGC	1 cut(s)	1000
Bsp19I	CCATGG	2 cut(s)	1266, 2232
Bsp68I	TCGCGA	1 cut(s)	1881
BspANI	GGCC	8 cut(s)	569, 889, 1265, 1317, 1814, 2328, 2333, 2867
BspCNI	CTCAG	8 cut(s)	75, 700, 1339, 1775, 1957, 2242, 2662, 2867
BspFNI	CGCG	5 cut(s)	111, 480, 1617, 1881, 3074
BspLI	GGNNCC	9 cut(s)	291, 429, 1560, 1594, 1645, 2602, 2760, 2939, 3053
BspMI	ACCTGC	1 cut(s)	448
BspPI	GGATC	8 cut(s)	587, 879, 1199, 1638, 1651, 1802, 2549, 3076
BspQI	GCTCTTC	1 cut(s)	1328
BspT107I	GGYRCC	2 cut(s)	427, 1592
BspTI	CTTAAG	1 cut(s)	377
BspTNI	GGTCTC	2 cut(s)	1863, 2971
BsrDI	GCAATG	4 cut(s)	1297, 2178, 2557, 2844
BsrFI	RCCGGY	3 cut(s)	574, 798, 3076
BsrI	ACTGG	9 cut(s)	565, 700, 1570, 1614, 1948, 2056, 2248, 2726, 2865
BssAI	RCCGGY	3 cut(s)	574, 798, 3076
BssECI	CCNNGG	8 cut(s)	1209, 1266, 1959, 2117, 2232, 2540, 2930, 2942
BssHII	GCGCGC	1 cut(s)	1615
BssNAI	GTATAC	1 cut(s)	2646
BssNI	GRCGYC	2 cut(s)	1392, 3015
BssSI	CACGAG	1 cut(s)	2106
BssT1I	CCWWGG	6 cut(s)	1209, 1266, 2117, 2232, 2930, 2942
Bst1107I	GTATAC	1 cut(s)	2646
Bst2BI	CACGAG	1 cut(s)	2106
Bst2UI	CCWGG	1 cut(s)	2088
Bst4CI	ACNGT	6 cut(s)	294, 614, 658, 1084, 2623, 2770
Bst6I	CTCTTC	2 cut(s)	1328, 2585
BstACI	GRCGYC	2 cut(s)	1392, 3015
BstAFI	CTTAAG	1 cut(s)	377
BstBAI	YACGTR	2 cut(s)	924, 2608
BstDSI	CCRYGG	3 cut(s)	1266, 1959, 2232
BstEII	GGTNACC	1 cut(s)	2470
BstF5I	GGATG	9 cut(s)	613, 890, 1079, 1219, 1255, 1783, 2148, 2293, 2997
BstFNI	CGCG	5 cut(s)	111, 480, 1617, 1881, 3074
BstH2I	RGCGCY	1 cut(s)	368
BstHHI	GCGC	5 cut(s)	367, 480, 1617, 1619, 3076
BstMAI	GTCTC	6 cut(s)	408, 480, 1684, 1863, 2712, 2971
BstMCI	CGRYCG	1 cut(s)	1902
BstMWI	GCNNNNNNNGC	8 cut(s)	160, 362, 371, 544, 959, 1567, 2264, 2724
BstNI	CCWGG	1 cut(s)	2088
BstNSI	RCATGY	3 cut(s)	65, 1261, 1423
BstPI	GGTNACC	1 cut(s)	2470
BstSCI	CCNGG	5 cut(s)	18, 457, 1554, 2086, 2328
BstSFI	CTRYAG	1 cut(s)	2304
BstSLI	GKGCMC	1 cut(s)	653
BstSNI	TACGTA	1 cut(s)	2608
BstUI	CGCG	5 cut(s)	111, 480, 1617, 1881, 3074
BstV1I	GCAGC	9 cut(s)	227, 681, 946, 1364, 1417, 1435, 1469, 2401, 2461
BstV2I	GAAGAC	4 cut(s)	968, 2910, 3010, 3149
BstX2I	RGATCY	3 cut(s)	871, 1328, 1643
BstYI	RGATCY	3 cut(s)	871, 1328, 1643
BstZ17I	GTATAC	1 cut(s)	2646
Bsu36I	CCTNAGG	1 cut(s)	1069
BsuRI	GGCC	8 cut(s)	569, 889, 1265, 1317, 1814, 2328, 2333, 2867
BtgI	CCRYGG	3 cut(s)	1266, 1959, 2232
BtgZI	GCGATG	2 cut(s)	1896, 2599
BtsCI	GGATG	9 cut(s)	613, 890, 1079, 1219, 1255, 1783, 2148, 2293, 2997
BtsI	GCAGTG	3 cut(s)	50, 1164, 2980
BtsIMutI	CAGTG	6 cut(s)	50, 588, 693, 705, 1164, 2980
BtuMI	TCGCGA	1 cut(s)	1881
BveI	ACCTGC	1 cut(s)	448
CaiI	CAGNNNCTG	1 cut(s)	2455
CfoI	GCGC	5 cut(s)	367, 480, 1617, 1619, 3076
Cfr10I	RCCGGY	3 cut(s)	574, 798, 3076
Cfr13I	GGNCC	2 cut(s)	2331, 2480
CseI	GACGC	2 cut(s)	117, 1400
Csp6I	GTAC	5 cut(s)	170, 925, 2301, 2365, 2858
CviQI	GTAC	5 cut(s)	170, 925, 2301, 2365, 2858
DraI	TTTAAA	1 cut(s)	522
DriI	GACNNNNNGTC	1 cut(s)	1583
EaeI	YGGCCR	3 cut(s)	887, 1315, 2865
Eam1104I	CTCTTC	2 cut(s)	1328, 2585
Eam1105I	GACNNNNNGTC	1 cut(s)	1583
EarI	CTCTTC	2 cut(s)	1328, 2585
EciI	GGCGGA	2 cut(s)	1233, 2300
Ecl136II	GAGCTC	1 cut(s)	1707
Eco105I	TACGTA	1 cut(s)	2608
Eco130I	CCWWGG	6 cut(s)	1209, 1266, 2117, 2232, 2930, 2942
Eco24I	GRGCYC	1 cut(s)	1709
Eco31I	GGTCTC	2 cut(s)	1863, 2971
Eco32I	GATATC	1 cut(s)	2373
Eco47I	GGWCC	1 cut(s)	2480
Eco47III	AGCGCT	1 cut(s)	366
Eco53kI	GAGCTC	1 cut(s)	1707
Eco57I	CTGAAG	2 cut(s)	1309, 2922
Eco81I	CCTNAGG	1 cut(s)	1069
Eco88I	CYCGRG	2 cut(s)	173, 1687
Eco91I	GGTNACC	1 cut(s)	2470
EcoICRI	GAGCTC	1 cut(s)	1707
EcoO65I	GGTNACC	1 cut(s)	2470
EcoRII	CCWGG	1 cut(s)	2086
EcoRV	GATATC	1 cut(s)	2373
EcoT14I	CCWWGG	6 cut(s)	1209, 1266, 2117, 2232, 2930, 2942
EcoT38I	GRGCYC	1 cut(s)	1709
ErhI	CCWWGG	6 cut(s)	1209, 1266, 2117, 2232, 2930, 2942
Esp3I	CGTCTC	1 cut(s)	408
FaqI	GGGAC	5 cut(s)	278, 1024, 1120, 2260, 2833
FblI	GTMKAC	1 cut(s)	2645
FokI	GGATG	9 cut(s)	620, 897, 1086, 1226, 1262, 1770, 2155, 2300, 2984
FriOI	GRGCYC	1 cut(s)	1709
FspBI	CTAG	4 cut(s)	71, 314, 1514, 2118
GlaI	GCGC	5 cut(s)	366, 479, 1616, 1618, 3075
GsaI	CCCAGC	1 cut(s)	2513
GsuI	CTGGAG	3 cut(s)	582, 1631, 1965
HaeII	RGCGCY	1 cut(s)	368
HaeIII	GGCC	8 cut(s)	569, 889, 1265, 1317, 1814, 2328, 2333, 2867
HgaI	GACGC	2 cut(s)	117, 1400
HhaI	GCGC	5 cut(s)	367, 480, 1617, 1619, 3076
Hin1I	GRCGYC	2 cut(s)	1392, 3015
Hin6I	GCGC	5 cut(s)	365, 478, 1615, 1617, 3074
HinP1I	GCGC	5 cut(s)	365, 478, 1615, 1617, 3074
HincII	GTYRAC	2 cut(s)	664, 1255
HindII	GTYRAC	2 cut(s)	664, 1255
HindIII	AAGCTT	4 cut(s)	1049, 2023, 2265, 2352
HinfI	GANTC	8 cut(s)	257, 266, 310, 395, 1180, 1692, 2226, 2842
HpaI	GTTAAC	1 cut(s)	1255
HphI	GGTGA	5 cut(s)	266, 473, 1948, 2929, 3091
Hpy166II	GTNNAC	8 cut(s)	664, 804, 1255, 1485, 1673, 1959, 2191, 2646
Hpy8I	GTNNAC	8 cut(s)	664, 804, 1255, 1485, 1673, 1959, 2191, 2646
Hpy99I	CGWCG	5 cut(s)	111, 188, 1664, 2387, 3095
HpyCH4III	ACNGT	6 cut(s)	294, 614, 658, 1084, 2623, 2770
HpyCH4IV	ACGT	4 cut(s)	923, 2382, 2607, 3015
HpyF10VI	GCNNNNNNNGC	8 cut(s)	160, 362, 371, 544, 959, 1567, 2264, 2724
HpySE526I	ACGT	4 cut(s)	923, 2382, 2607, 3015
Hsp92I	GRCGYC	2 cut(s)	1392, 3015
HspAI	GCGC	5 cut(s)	365, 478, 1615, 1617, 3074
KspAI	GTTAAC	1 cut(s)	1255
LguI	GCTCTTC	1 cut(s)	1328
LmnI	GCTCC	4 cut(s)	1558, 1612, 2764, 3057
Lsp1109I	GCAGC	9 cut(s)	227, 681, 946, 1364, 1417, 1435, 1469, 2401, 2461
LweI	GCATC	9 cut(s)	375, 908, 1064, 1285, 2133, 2185, 2622, 2637, 2676
MaeI	CTAG	4 cut(s)	71, 314, 1514, 2118
MaeII	ACGT	4 cut(s)	923, 2382, 2607, 3015
MaeIII	GTNAC	9 cut(s)	57, 461, 614, 1132, 1415, 1835, 2470, 2681, 2807
MfeI	CAATTG	2 cut(s)	36, 813
MflI	RGATCY	3 cut(s)	871, 1328, 1643
MhlI	GDGCHC	3 cut(s)	89, 653, 1709
MlsI	TGGCCA	3 cut(s)	889, 1317, 2867
MluNI	TGGCCA	3 cut(s)	889, 1317, 2867
MlyI	GAGTC	5 cut(s)	260, 266, 404, 1686, 2836
MmeI	TCCRAC	5 cut(s)	313, 463, 2365, 2762, 2823
Mox20I	TGGCCA	3 cut(s)	889, 1317, 2867
MroXI	GAANNNNTTC	4 cut(s)	453, 975, 2099, 2354
MscI	TGGCCA	3 cut(s)	889, 1317, 2867
MslI	CAYNNNNRTG	8 cut(s)	28, 194, 509, 575, 698, 2345, 2675, 2873
Msp20I	TGGCCA	3 cut(s)	889, 1317, 2867
MspA1I	CMGCKG	5 cut(s)	672, 2315, 2414, 2513, 2756
MspCI	CTTAAG	1 cut(s)	377
MspR9I	CCNGG	5 cut(s)	20, 459, 1556, 2088, 2330
MunI	CAATTG	2 cut(s)	36, 813
Mva1269I	GAATGC	1 cut(s)	1039
MvaI	CCWGG	1 cut(s)	2088
MvnI	CGCG	5 cut(s)	111, 480, 1617, 1881, 3074
MwoI	GCNNNNNNNGC	8 cut(s)	160, 362, 371, 544, 959, 1567, 2264, 2724
NciI	CCSGG	4 cut(s)	20, 459, 1556, 2330
NcoI	CCATGG	2 cut(s)	1266, 2232
NlaIV	GGNNCC	9 cut(s)	291, 429, 1560, 1594, 1645, 2602, 2760, 2939, 3053
NmeAIII	GCCGAG	1 cut(s)	2137
NmuCI	GTSAC	7 cut(s)	461, 614, 1132, 1415, 1835, 2681, 2807
NruI	TCGCGA	1 cut(s)	1881
NspI	RCATGY	3 cut(s)	65, 1261, 1423
OliI	CACNNNNGTG	2 cut(s)	194, 2675
PaeR7I	CTCGAG	2 cut(s)	173, 1687
PauI	GCGCGC	1 cut(s)	1615
PciI	ACATGT	1 cut(s)	61
PciSI	GCTCTTC	1 cut(s)	1328
PcsI	WCGNNNNNNNCGW	3 cut(s)	180, 1180, 1656
PctI	GAATGC	1 cut(s)	1039
PdmI	GAANNNNTTC	4 cut(s)	453, 975, 2099, 2354
PfeI	GAWTC	3 cut(s)	310, 1180, 2226
PflFI	GACNNNGTC	1 cut(s)	2845
PfoI	TCCNGGA	1 cut(s)	1554
Ple19I	CGATCG	1 cut(s)	1902
PleI	GAGTC	5 cut(s)	260, 265, 403, 1686, 2836
PpsI	GAGTC	5 cut(s)	260, 265, 403, 1686, 2836
Ppu21I	YACGTR	2 cut(s)	924, 2608
PscI	ACATGT	1 cut(s)	61
Psp124BI	GAGCTC	1 cut(s)	1709
Psp6I	CCWGG	1 cut(s)	2086
PspEI	GGTNACC	1 cut(s)	2470
PspFI	CCCAGC	1 cut(s)	2509
PspGI	CCWGG	1 cut(s)	2086
PspN4I	GGNNCC	9 cut(s)	291, 429, 1560, 1594, 1645, 2602, 2760, 2939, 3053
PspPI	GGNCC	2 cut(s)	2331, 2480
PspXI	VCTCGAGB	1 cut(s)	173
PstNI	CAGNNNCTG	1 cut(s)	2455
PsuI	RGATCY	3 cut(s)	871, 1328, 1643
PsyI	GACNNNGTC	1 cut(s)	2845
PteI	GCGCGC	1 cut(s)	1615
PvuI	CGATCG	1 cut(s)	1902
PvuII	CAGCTG	2 cut(s)	672, 2513
RruI	TCGCGA	1 cut(s)	1881
RsaI	GTAC	5 cut(s)	171, 926, 2302, 2366, 2859
RsaNI	GTAC	5 cut(s)	170, 925, 2301, 2365, 2858
RseI	CAYNNNNRTG	8 cut(s)	28, 194, 509, 575, 698, 2345, 2675, 2873
SacI	GAGCTC	1 cut(s)	1709
SapI	GCTCTTC	1 cut(s)	1328
Sau96I	GGNCC	2 cut(s)	2331, 2480
ScaI	AGTACT	2 cut(s)	2302, 2859
SchI	GAGTC	5 cut(s)	260, 266, 404, 1686, 2836
ScrFI	CCNGG	5 cut(s)	20, 459, 1556, 2088, 2330
SduI	GDGCHC	3 cut(s)	89, 653, 1709
SfaNI	GCATC	9 cut(s)	375, 908, 1064, 1285, 2133, 2185, 2622, 2637, 2676
SfcI	CTRYAG	1 cut(s)	2304
Sfr274I	CTCGAG	2 cut(s)	173, 1687
SgrAI	CRCCGGYG	1 cut(s)	3076
SinI	GGWCC	1 cut(s)	2480
SlaI	CTCGAG	2 cut(s)	173, 1687
SmiMI	CAYNNNNRTG	8 cut(s)	28, 194, 509, 575, 698, 2345, 2675, 2873
SmlI	CTYRAG	4 cut(s)	173, 377, 1358, 1687
SmoI	CTYRAG	4 cut(s)	173, 377, 1358, 1687
SnaBI	TACGTA	1 cut(s)	2608
SspMI	CTAG	4 cut(s)	71, 314, 1514, 2118
SstI	GAGCTC	1 cut(s)	1709
StyD4I	CCNGG	5 cut(s)	18, 457, 1554, 2086, 2328
StyI	CCWWGG	6 cut(s)	1209, 1266, 2117, 2232, 2930, 2942
TaaI	ACNGT	6 cut(s)	294, 614, 658, 1084, 2623, 2770
TaiI	ACGT	4 cut(s)	926, 2385, 2610, 3018
TaqII	GACCGA	1 cut(s)	2953
TatI	WGTACW	3 cut(s)	169, 2300, 2857
TauI	GCSGC	2 cut(s)	166, 2759
TfiI	GAWTC	3 cut(s)	310, 1180, 2226
TscAI	CASTG	6 cut(s)	50, 595, 700, 712, 1171, 2987
TseFI	GTSAC	7 cut(s)	461, 614, 1132, 1415, 1835, 2681, 2807
TseI	GCWGC	9 cut(s)	215, 669, 959, 1352, 1423, 1430, 1457, 2414, 2449
Tsp45I	GTSAC	7 cut(s)	461, 614, 1132, 1415, 1835, 2681, 2807
TspGWI	ACGGA	2 cut(s)	2700, 3021
TspRI	CASTG	6 cut(s)	50, 595, 700, 712, 1171, 2987
Tth111I	GACNNNGTC	1 cut(s)	2845
Vha464I	CTTAAG	1 cut(s)	377
VpaK11BI	GGWCC	1 cut(s)	2480
XapI	RAATTY	3 cut(s)	788, 1410, 1517
XceI	RCATGY	3 cut(s)	65, 1261, 1423
XhoI	CTCGAG	2 cut(s)	173, 1687
XmaJI	CCTAGG	1 cut(s)	2117
XmiI	GTMKAC	1 cut(s)	2645
XmnI	GAANNNNTTC	4 cut(s)	453, 975, 2099, 2354
XspI	CTAG	4 cut(s)	71, 314, 1514, 2118
ZraI	GACGTC	1 cut(s)	3016
ZrmI	AGTACT	2 cut(s)	2302, 2859

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.