FvH4_7g18450

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016921
Physical Location & Seq
Reverse (-)
15418190 .. 15419941
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g18450.t1

Sequence Viewer

Length: 1752 bp
ATGAAGAGCCTTGTCCTGCAAGTCCTCAAAGGACGATGGCTGATTGTGTTTGCTTCATTTCTGATGATGGTCACAGCCGGTGCCAGCTACATGTTCGGCCTCTACTCCAACGACATCAAGTCTGTCCTCGGCTACGACCAAACCACCCTCAACCTCATCAGCTTCTCCAAAGACCTAGGTGCCAACATTGGCATCCTCTCCGGCCTCATCAACGAAGTCACACCACCCTGGGTGGTCTTATCAATCGGTGCAGTCCTCAACTTCTTCGGGCACTTCATGATTTGGCTAGCAGTAGCTCAAAAAATCCATAAACCCCAAGTTTGGCACATGTGCATGTACATGGGCATAGGAGCTAACTCACACACTTTCACCAACACCGGAGCTCTGGTCACCTGCGTGAAGAACTTCCCGGAGAGCCGTGGCATCGTGCTAGGGCTTTTGAAGAGCTATACTGGTATAAGTGCAGCTGTGGTGGCACAGCTGTACCATGCCGTCTACGGTGACGATACCAAGTATTTCACTTTGGTTGTGGCCTGGCTTCCGACAGCTATTTCCTTCGCCTTCATCGGAACTATTCGGATCATGAAGGTTAGTCGACGTGGCTCGAACGAGCTCAAGGCTTTTTATCACTTCCTCTATCTGTCACTAGGTCTAGCTGCATTTTTGTTGGTTATAATTATAGTGGAAAAAAGGGTCACGTTTAGTCCAAGTGAGTATGTTGGAAGTGCAGCTGTAGTCCTCTTTTTACTCTTTTTACCACTTACCGTGGTAATTATGGAAGAGTACAAGGTTTGGGAGAGCAAGAGATCAATAGTAACTAAAGATTCTAGCCATGCCAAACCGGAGATTTTGGGGCATCGAGATGAACCATTAGAACATTTTGTTGTCATTGAAAAAGAAGTTTCTTGGCGGAAAGACATATTTAACCCGCCGGAGATCGGAGAGGACTTCACCATACTTCAAGCCGTTTTCAGCGTCGAGATGGTGACTTTACTCTTTGCAACAATTTGTGGACTTGGAGGCACCATGACCATGATGGACAACTTGGGACAAATTGGTACATCTCTTGGGTACCCTCTGAAAAGCATAAGAAACTTTGTGTCCCTAACAAGCATATGGAACTATTCAGGTCATATTGCCGCTGGTATAGGCTCAGAAATATTCATCACAAAGTACAAATGGCCTCGCCCTCTAATTTTCACAGCAATTCTCTTGCTCTCGTGTGTTGGTCATCTTCTAATAGCGTTCAACATCCCATATGGTGTCTATGTTGCTTCAGTGGTCACTGGTTTTTGTTTTGGTGCTCATTGGCCTTTGATCTACACCATTATATCCGAGCTTTTCGGCCTCAAGTACTACTCGACTTTGTACAATATTGGTGGATTGGCTAGCCCTATTGGGTTGTATGTGCTCAATGTGAGGGTTACGGGGCATTTGTATGATGTGGAGGCTAAGAAGCAAATGGCAGCTTTGGGGATTCAGAGGAAGGTTGGAGAGGAGTTGAACTGTGTTGGGGGAGAGTGCTTCAAATGGTCTTTCATCATTATTACTGTGGTAACTTTTGTTGGAACACTAGTTTCTTTGGTTTTGGTGGTGAGGACAAGGAAGTTTTATAAGGGTGATATTTACAAGAAGTTTAGAGAAACGGTTAGTGTGGCTGATGAAACGAAATTAGAGGTTGCTAAAACTTCTGCTGGAGTGGAAACTACTGGTCTCAAACATGACCTCTCCAAGCCCAGAGGTCAATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

584

Amino Acids

64.72

Weight (kDa)

9.07

Isoelectric Point (pI)

26.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 12 - 258 1.3e-86 Nodulin-like
MFS_1 PF07690 245 - 465 7e-07 Major Facilitator Superfamily
NFD4_C PF23262 339 - 538 1.8e-15 NFD4, C-terminal domain
MFS_1 PF07690 393 - 536 6.6e-07 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016921)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18450
malus_domestica MD01G1101200.v1.1
prunus_persica Prupe.2G206400_v2.0.a1
pyrus_communis pycom01g12780
rosa_chinensis RchiOBHm_Chr1g0362251
rosa_laevigata RLG00000027663
rosa_multiflora Rmu_sc0001237.1_g000013 Rmu_ssc0000009.1_g000001
rosa_roxburghii Rroxscaffold_4G00294400
rosa_rugosa Rorug01G0296000
rosa_samantha Rh1AG305400 Rh1BG268400 Rh1CG286500 Rh1DG298500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 674, 1614
AarI CACCTGC 1 cut(s) 401
Acc36I ACCTGC 1 cut(s) 401
Acc65I GGTACC 1 cut(s) 1071
AccB1I GGYRCC 4 cut(s) 80, 179, 1022, 1071
AccI GTMKAC 2 cut(s) 495, 595
AciI CCGC 3 cut(s) 910, 929, 1140
AclWI GGATC 1 cut(s) 587
AcuI CTGAAG 1 cut(s) 1260
AfaI GTAC 8 cut(s) 338, 485, 785, 1060, 1073, 1175, 1355, 1370
AfiI CCNNNNNNNGG 2 cut(s) 321, 938
AflIII ACRYGT 2 cut(s) 90, 327
AgsI TTSAA 6 cut(s) 442, 893, 962, 1249, 1504, 1528
AhdI GACNNNNNGTC 1 cut(s) 118
AhlI ACTAGT 1 cut(s) 1573
AjiI CACGTC 1 cut(s) 599
AjnI CCWGG 2 cut(s) 227, 533
AleI CACNNNNGTG 1 cut(s) 395
Alw21I GWGCWC 4 cut(s) 385, 615, 1306, 1413
Alw26I GTCTC 1 cut(s) 1718
AlwI GGATC 1 cut(s) 587
AoxI GGCC 6 cut(s) 97, 202, 531, 1181, 1310, 1345
ApeKI GCWGC 4 cut(s) 464, 656, 728, 1466
Asp700I GAANNNNTTC 1 cut(s) 404
Asp718I GGTACC 1 cut(s) 1071
AspA2I CCTAGG 1 cut(s) 175
AsuC2I CCSGG 1 cut(s) 410
AsuHPI GGTGA 7 cut(s) 361, 382, 512, 943, 997, 1606, 1631
AsuNHI GCTAGC 2 cut(s) 286, 1388
AvrII CCTAGG 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 273
BaeI ACNNNNGTAYC 2 cut(s) 467, 500
BanI GGYRCC 4 cut(s) 80, 179, 1022, 1071
BanII GRGCYC 2 cut(s) 385, 615
BauI CACGAG 1 cut(s) 1219
Bbv12I GWGCWC 4 cut(s) 385, 615, 1306, 1413
BbvI GCAGC 4 cut(s) 476, 643, 740, 1478
BccI CCATC 4 cut(s) 30, 61, 976, 1030
BceAI ACGGC 3 cut(s) 402, 476, 950
BciT130I CCWGG 2 cut(s) 229, 535
BcnI CCSGG 1 cut(s) 410
BcoDI GTCTC 1 cut(s) 1718
BcuI ACTAGT 1 cut(s) 1573
BfaI CTAG 8 cut(s) 176, 287, 431, 647, 653, 828, 1389, 1574
BfmI CTRYAG 1 cut(s) 732
BfuAI ACCTGC 1 cut(s) 401
BisI GCNGC 5 cut(s) 465, 657, 729, 1140, 1467
BlnI CCTAGG 1 cut(s) 175
BlsI GCNGC 5 cut(s) 466, 658, 730, 1141, 1468
BmcAI AGTACT 1 cut(s) 1355
Bme1390I CCNGG 3 cut(s) 229, 410, 535
BmeRI GACNNNNNGTC 1 cut(s) 118
BmgBI CACGTC 1 cut(s) 599
BmiI GGNNCC 4 cut(s) 82, 181, 1024, 1073
BmrFI CCNGG 3 cut(s) 229, 410, 535
BmsI GCATC 3 cut(s) 201, 432, 865
BmtI GCTAGC 2 cut(s) 290, 1392
BpmI CTGGAG 1 cut(s) 1716
BpuEI CTTGAG 2 cut(s) 599, 1334
BpuMI CCSGG 1 cut(s) 410
BsaI GGTCTC 1 cut(s) 1718
BsaJI CCNNGG 6 cut(s) 127, 175, 227, 228, 418, 765
BsaWI WCCGGW 2 cut(s) 377, 841
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bsc4I CCNNNNNNNGG 2 cut(s) 321, 938
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 3 cut(s) 457, 1291, 1714
BseBI CCWGG 2 cut(s) 229, 535
BseDI CCNNGG 6 cut(s) 127, 175, 227, 228, 418, 765
BseGI GGATG 2 cut(s) 192, 1251
BseLI CCNNNNNNNGG 2 cut(s) 321, 938
BseMII CTCAG 1 cut(s) 1167
BseNI ACTGG 3 cut(s) 457, 1291, 1714
BseRI GAGGAG 1 cut(s) 1511
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 4 cut(s) 476, 643, 740, 1478
BsgI GTGCAG 3 cut(s) 270, 483, 747
BshFI GGCC 6 cut(s) 99, 204, 533, 1183, 1312, 1347
BshNI GGYRCC 4 cut(s) 80, 179, 1022, 1071
BsiHKAI GWGCWC 4 cut(s) 385, 615, 1306, 1413
BsiSI CCGG 6 cut(s) 78, 201, 378, 410, 842, 932
BslFI GGGAC 2 cut(s) 1062, 1087
BslI CCNNNNNNNGG 2 cut(s) 321, 938
BsmAI GTCTC 1 cut(s) 1718
BsmFI GGGAC 2 cut(s) 1062, 1087
BsnI GGCC 6 cut(s) 99, 204, 533, 1183, 1312, 1347
Bso31I GGTCTC 1 cut(s) 1718
Bsp1286I GDGCHC 5 cut(s) 273, 385, 615, 1306, 1413
Bsp1407I TGTACA 2 cut(s) 336, 1368
Bsp143I GATC 4 cut(s) 579, 806, 936, 1317
BspACI CCGC 3 cut(s) 910, 929, 1140
BspANI GGCC 6 cut(s) 99, 204, 533, 1183, 1312, 1347
BspCNI CTCAG 1 cut(s) 1166
BspHI TCATGA 2 cut(s) 276, 582
BspLI GGNNCC 4 cut(s) 82, 181, 1024, 1073
BspMI ACCTGC 1 cut(s) 401
BspOI GCTAGC 2 cut(s) 290, 1392
BspPI GGATC 1 cut(s) 587
BspQI GCTCTTC 1 cut(s) 437
BspT107I GGYRCC 4 cut(s) 80, 179, 1022, 1071
BspTNI GGTCTC 1 cut(s) 1718
BsrFI RCCGGY 1 cut(s) 77
BsrGI TGTACA 2 cut(s) 336, 1368
BsrI ACTGG 3 cut(s) 457, 1291, 1714
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 6 cut(s) 127, 175, 227, 228, 418, 765
BssMI GATC 4 cut(s) 579, 806, 936, 1317
BssSI CACGAG 1 cut(s) 1219
BssT1I CCWWGG 1 cut(s) 175
Bst2BI CACGAG 1 cut(s) 1219
Bst2UI CCWGG 2 cut(s) 229, 535
Bst4CI ACNGT 5 cut(s) 500, 766, 1508, 1552, 1648
Bst6I CTCTTC 2 cut(s) 437, 774
BstAUI TGTACA 2 cut(s) 336, 1368
BstC8I GCNNGC 3 cut(s) 85, 288, 1390
BstDEI CTNAG 2 cut(s) 1153, 1452
BstDSI CCRYGG 2 cut(s) 418, 765
BstEII GGTNACC 1 cut(s) 388
BstF5I GGATG 2 cut(s) 192, 1251
BstKTI GATC 4 cut(s) 582, 809, 939, 1320
BstMAI GTCTC 1 cut(s) 1718
BstMBI GATC 4 cut(s) 579, 806, 936, 1317
BstMWI GCNNNNNNNGC 1 cut(s) 473
BstNI CCWGG 2 cut(s) 229, 535
BstNSI RCATGY 3 cut(s) 94, 331, 337
BstPI GGTNACC 1 cut(s) 388
BstSCI CCNGG 3 cut(s) 227, 408, 533
BstSFI CTRYAG 1 cut(s) 732
BstSLI GKGCMC 1 cut(s) 273
BstV1I GCAGC 4 cut(s) 476, 643, 740, 1478
BsuRI GGCC 6 cut(s) 99, 204, 533, 1183, 1312, 1347
BtgI CCRYGG 2 cut(s) 418, 765
BtrI CACGTC 1 cut(s) 599
BtsCI GGATG 2 cut(s) 192, 1251
BtsIMutI CAGTG 2 cut(s) 1284, 1284
BveI ACCTGC 1 cut(s) 401
Cac8I GCNNGC 3 cut(s) 85, 288, 1390
CciI TCATGA 2 cut(s) 276, 582
Cfr10I RCCGGY 1 cut(s) 77
CseI GACGC 1 cut(s) 964
Csp6I GTAC 8 cut(s) 337, 484, 784, 1059, 1072, 1174, 1354, 1369
CspCI CAANNNNNGTGG 2 cut(s) 1360, 1395
CviQI GTAC 8 cut(s) 337, 484, 784, 1059, 1072, 1174, 1354, 1369
DdeI CTNAG 2 cut(s) 1153, 1452
DpnI GATC 4 cut(s) 581, 808, 938, 1319
DpnII GATC 4 cut(s) 579, 806, 936, 1317
DriI GACNNNNNGTC 1 cut(s) 118
Eam1104I CTCTTC 2 cut(s) 437, 774
Eam1105I GACNNNNNGTC 1 cut(s) 118
EarI CTCTTC 2 cut(s) 437, 774
EciI GGCGGA 1 cut(s) 925
Ecl136II GAGCTC 2 cut(s) 383, 613
Eco130I CCWWGG 1 cut(s) 175
Eco24I GRGCYC 2 cut(s) 385, 615
Eco31I GGTCTC 1 cut(s) 1718
Eco53kI GAGCTC 2 cut(s) 383, 613
Eco57I CTGAAG 1 cut(s) 1260
Eco91I GGTNACC 1 cut(s) 388
EcoICRI GAGCTC 2 cut(s) 383, 613
EcoO65I GGTNACC 1 cut(s) 388
EcoRII CCWGG 2 cut(s) 227, 533
EcoT14I CCWWGG 1 cut(s) 175
EcoT38I GRGCYC 2 cut(s) 385, 615
ErhI CCWWGG 1 cut(s) 175
FaqI GGGAC 2 cut(s) 1062, 1087
FauI CCCGC 1 cut(s) 936
FauNDI CATATG 2 cut(s) 1115, 1258
FblI GTMKAC 2 cut(s) 495, 595
Fnu4HI GCNGC 5 cut(s) 465, 657, 729, 1140, 1467
FokI GGATG 2 cut(s) 179, 1238
FriOI GRGCYC 2 cut(s) 385, 615
Fsp4HI GCNGC 5 cut(s) 465, 657, 729, 1140, 1467
FspBI CTAG 8 cut(s) 176, 287, 431, 647, 653, 828, 1389, 1574
GluI GCNGC 5 cut(s) 465, 657, 729, 1140, 1467
GsuI CTGGAG 1 cut(s) 1716
HaeIII GGCC 6 cut(s) 99, 204, 533, 1183, 1312, 1347
HapII CCGG 6 cut(s) 78, 201, 378, 410, 842, 932
HgaI GACGC 1 cut(s) 964
HincII GTYRAC 1 cut(s) 596
HindII GTYRAC 1 cut(s) 596
HinfI GANTC 2 cut(s) 824, 1477
HpaII CCGG 6 cut(s) 78, 201, 378, 410, 842, 932
HphI GGTGA 7 cut(s) 361, 382, 512, 943, 997, 1606, 1631
Hpy166II GTNNAC 3 cut(s) 496, 596, 1013
Hpy188I TCNGA 9 cut(s) 63, 543, 569, 579, 941, 1080, 1156, 1336, 1482
Hpy188III TCNNGA 4 cut(s) 277, 583, 860, 979
Hpy8I GTNNAC 3 cut(s) 496, 596, 1013
Hpy99I CGWCG 2 cut(s) 600, 980
HpyAV CCTTC 4 cut(s) 565, 571, 580, 1480
HpyCH4III ACNGT 5 cut(s) 500, 766, 1508, 1552, 1648
HpyCH4IV ACGT 2 cut(s) 598, 698
HpyCH4V TGCA 7 cut(s) 19, 251, 333, 464, 659, 728, 1001
HpyF10VI GCNNNNNNNGC 1 cut(s) 473
HpyF3I CTNAG 2 cut(s) 1153, 1452
HpySE526I ACGT 2 cut(s) 598, 698
KpnI GGTACC 1 cut(s) 1075
Kzo9I GATC 4 cut(s) 579, 806, 936, 1317
LguI GCTCTTC 1 cut(s) 437
LmnI GCTCC 2 cut(s) 350, 380
Lsp1109I GCAGC 4 cut(s) 476, 643, 740, 1478
LweI GCATC 3 cut(s) 201, 432, 865
MaeI CTAG 8 cut(s) 176, 287, 431, 647, 653, 828, 1389, 1574
MaeII ACGT 2 cut(s) 598, 698
MalI GATC 4 cut(s) 581, 808, 938, 1319
MboI GATC 4 cut(s) 579, 806, 936, 1317
MboII GAAGA 6 cut(s) 16, 256, 412, 454, 791, 1226
MhlI GDGCHC 5 cut(s) 273, 385, 615, 1306, 1413
MluCI AATT 7 cut(s) 675, 771, 1005, 1053, 1194, 1206, 1670
MmeI TCCRAC 5 cut(s) 132, 566, 700, 1471, 1546
MroXI GAANNNNTTC 1 cut(s) 404
MseI TTAA 1 cut(s) 924
MslI CAYNNNNRTG 6 cut(s) 332, 338, 395, 861, 1031, 1437
MspA1I CMGCKG 4 cut(s) 467, 481, 731, 1142
MspI CCGG 6 cut(s) 78, 201, 378, 410, 842, 932
MspR9I CCNGG 3 cut(s) 229, 410, 535
MvaI CCWGG 2 cut(s) 229, 535
MwoI GCNNNNNNNGC 1 cut(s) 473
NciI CCSGG 1 cut(s) 410
NdeI CATATG 2 cut(s) 1115, 1258
NdeII GATC 4 cut(s) 579, 806, 936, 1317
NheI GCTAGC 2 cut(s) 286, 1388
NlaIV GGNNCC 4 cut(s) 82, 181, 1024, 1073
NmeAIII GCCGAG 1 cut(s) 108
NmuCI GTSAC 8 cut(s) 70, 217, 388, 500, 642, 694, 985, 1282
NspI RCATGY 3 cut(s) 94, 331, 337
OliI CACNNNNGTG 1 cut(s) 395
PagI TCATGA 2 cut(s) 276, 582
PaqCI CACCTGC 1 cut(s) 401
PasI CCCWGGG 1 cut(s) 228
PciI ACATGT 2 cut(s) 90, 327
PciSI GCTCTTC 1 cut(s) 437
PdmI GAANNNNTTC 1 cut(s) 404
PfeI GAWTC 2 cut(s) 824, 1477
PfoI TCCNGGA 1 cut(s) 408
PkrI GCNGC 5 cut(s) 466, 658, 730, 1141, 1468
PscI ACATGT 2 cut(s) 90, 327
PsiI TTATAA 2 cut(s) 674, 1614
Psp124BI GAGCTC 2 cut(s) 385, 615
Psp6I CCWGG 2 cut(s) 227, 533
PspEI GGTNACC 1 cut(s) 388
PspGI CCWGG 2 cut(s) 227, 533
PspN4I GGNNCC 4 cut(s) 82, 181, 1024, 1073
PvuII CAGCTG 3 cut(s) 467, 481, 731
RsaI GTAC 8 cut(s) 338, 485, 785, 1060, 1073, 1175, 1355, 1370
RsaNI GTAC 8 cut(s) 337, 484, 784, 1059, 1072, 1174, 1354, 1369
RseI CAYNNNNRTG 6 cut(s) 332, 338, 395, 861, 1031, 1437
SacI GAGCTC 2 cut(s) 385, 615
SalI GTCGAC 1 cut(s) 594
SapI GCTCTTC 1 cut(s) 437
SaqAI TTAA 1 cut(s) 924
SatI GCNGC 5 cut(s) 465, 657, 729, 1140, 1467
Sau3AI GATC 4 cut(s) 579, 806, 936, 1317
ScaI AGTACT 1 cut(s) 1355
ScrFI CCNGG 3 cut(s) 229, 410, 535
SduI GDGCHC 5 cut(s) 273, 385, 615, 1306, 1413
SfaNI GCATC 3 cut(s) 201, 432, 865
SfcI CTRYAG 1 cut(s) 732
SmiMI CAYNNNNRTG 6 cut(s) 332, 338, 395, 861, 1031, 1437
SmlI CTYRAG 2 cut(s) 614, 1349
SmoI CTYRAG 2 cut(s) 614, 1349
SpeI ACTAGT 1 cut(s) 1573
Sse9I AATT 7 cut(s) 675, 771, 1005, 1053, 1194, 1206, 1670
SsiI CCGC 3 cut(s) 910, 929, 1140
SspI AATATT 2 cut(s) 1161, 1375
SspMI CTAG 8 cut(s) 176, 287, 431, 647, 653, 828, 1389, 1574
SstI GAGCTC 2 cut(s) 385, 615
StyD4I CCNGG 3 cut(s) 227, 408, 533
StyI CCWWGG 1 cut(s) 175
TaaI ACNGT 5 cut(s) 500, 766, 1508, 1552, 1648
TaiI ACGT 2 cut(s) 601, 701
TaqI TCGA 5 cut(s) 595, 605, 859, 978, 1361
TasI AATT 7 cut(s) 675, 771, 1005, 1053, 1194, 1206, 1670
TatI WGTACW 5 cut(s) 336, 783, 1173, 1353, 1368
TauI GCSGC 1 cut(s) 1142
TfiI GAWTC 2 cut(s) 824, 1477
Tru1I TTAA 1 cut(s) 924
Tru9I TTAA 1 cut(s) 924
TscAI CASTG 2 cut(s) 1284, 1291
TseFI GTSAC 8 cut(s) 70, 217, 388, 500, 642, 694, 985, 1282
TseI GCWGC 4 cut(s) 464, 656, 728, 1466
Tsp45I GTSAC 8 cut(s) 70, 217, 388, 500, 642, 694, 985, 1282
TspDTI ATGAA 9 cut(s) 17, 45, 265, 553, 599, 879, 1153, 1528, 1677
TspRI CASTG 2 cut(s) 1284, 1291
XceI RCATGY 3 cut(s) 94, 331, 337
XcmI CCANNNNNNNNNTGG 1 cut(s) 1033
XmaJI CCTAGG 1 cut(s) 175
XmiI GTMKAC 2 cut(s) 495, 595
XmnI GAANNNNTTC 1 cut(s) 404
XspI CTAG 8 cut(s) 176, 287, 431, 647, 653, 828, 1389, 1574
ZrmI AGTACT 1 cut(s) 1355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.