MD01G1101200.v1.1

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0016921
Physical Location & Seq
Reverse (-)
21416861 .. 21418069
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1101200.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGGAGGTGCGCAGTTGTGTGGACATGAAGGGCCTTGTCCTACAAGTCCTCAAAGGAAGATGGCTCATGGTTTTTGCTTCATTCCTAATGATGGTCACTGCCGGAGCAAGCTACATGTTCGGCCTCTATTCCAACGACATCAAGTCCGTCCTTGGCTATGACCAAACGACACTCAACTTAATCAGCTTCTTCAAGGACTTAGGAGGCAACATTGGCATCTTTTCCGGCCTAATCAACGAGGTCACACCGCCTTGGGTGGCCTTATCAATCGGTGCAGTCCTAAACTTTTTCGGACACTTCATGATTTGGCTTGCTGTTACACAAAAAATCCCCAAACCAAAAGTTTGGCACATGTGTATGTACATCTCTATAGGGTCAAATTCACATACTTTCACCAACACTGGAGCTCTTGTCACCTGTGTTAAGAACTTCCCCGAAAGCCGGGGTGTTGTGTTAGGCCTTTTGAAAAGCTATACCGGTATAAGTGCAGCTGTGATTGCACAGCTCTACCATGCTGCCTATGGCGATGACACAAAGTCCTTCACTTTGTTGGTGGCTTGGCTTCCTACAGCAATCTCTTTCGCTTTCATTGGAACCATTCGGATCATGAAGGTTAGTCGCCGCCCGAATGAGCTCAAAGCTTTCTATAATTTCCTTTATATTTCACTTGCCCTAGCAGCATTTTTGCTGATTATAATTATTGTTGAGAAAAGTTTTAGGTTTAATCAAAGAGAGTATGTTGGAAGTGCAACTGTTGTGCTCTTTTTGCTCTTTTTACCACTTGGTGTGGTAATTATGGAAGAATATAAAGTCTGGAAGAGCAAGCGAGATTCAAACCAAGAAACCATTTCCTCTCCAACCCCACAGTTATCATTTACTGTTCCATACAAAAAAGTTTCACCCTGGAACAAGAATATTTTTACCCCACCAGAAATTGGTGAGGACTTCACTATTTTGCAAGCACTTTTCAGCATTGAAATGCTGACCTTACTGTTGGCAACAATCTGTGGCCTTGGAGGCACAATGACCATGATGGACAACTTGGGGCAGATTGGAACTTCTTTTGGATACCCCTTACGAAGCATCCGATACTTTGTATCCCTTACAAGCATATGGAATTATTTAGGGCAAATATCCGCTGGTATAGGCTCCGAAATCTTCATCACAAAGTACAAATGGCCTCGGCCTCTCATCTTTGTTAGAACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

403

Amino Acids

45.0

Weight (kDa)

9.44

Isoelectric Point (pI)

30.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 20 - 265 6.9e-89 Nodulin-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016921)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18450
malus_domestica MD01G1101200.v1.1
prunus_persica Prupe.2G206400_v2.0.a1
pyrus_communis pycom01g12780
rosa_chinensis RchiOBHm_Chr1g0362251
rosa_laevigata RLG00000027663
rosa_multiflora Rmu_sc0001237.1_g000013 Rmu_ssc0000009.1_g000001
rosa_roxburghii Rroxscaffold_4G00294400
rosa_rugosa Rorug01G0296000
rosa_samantha Rh1AG305400 Rh1BG268400 Rh1CG286500 Rh1DG298500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 695
Acc16I TGCGCA 1 cut(s) 11
AccB7I CCANNNNNTGG 1 cut(s) 935
AciI CCGC 3 cut(s) 248, 622, 1137
AclWI GGATC 1 cut(s) 611
AcsI RAATTY 1 cut(s) 379
AdeI CACNNNGTG 1 cut(s) 785
AfaI GTAC 2 cut(s) 362, 1172
AfiI CCNNNNNNNGG 3 cut(s) 91, 441, 935
AflIII ACRYGT 2 cut(s) 114, 351
AgeI ACCGGT 1 cut(s) 476
AgsI TTSAA 4 cut(s) 193, 466, 834, 977
AhdI GACNNNNNGTC 2 cut(s) 142, 535
AjnI CCWGG 1 cut(s) 902
AluBI AGCT 8 cut(s) 111, 186, 407, 471, 491, 505, 634, 641
AluI AGCT 8 cut(s) 111, 186, 407, 471, 491, 505, 634, 641
Alw21I GWGCWC 3 cut(s) 409, 636, 762
AlwI GGATC 1 cut(s) 611
AoxI GGCC 8 cut(s) 31, 121, 226, 258, 457, 1009, 1178, 1184
ApeKI GCWGC 3 cut(s) 488, 515, 677
ApoI RAATTY 1 cut(s) 379
AsiGI ACCGGT 1 cut(s) 476
AspLEI GCGC 1 cut(s) 12
AspS9I GGNCC 1 cut(s) 31
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 4 cut(s) 385, 406, 891, 950
BanII GRGCYC 2 cut(s) 409, 636
Bbv12I GWGCWC 3 cut(s) 409, 636, 762
BbvI GCAGC 3 cut(s) 500, 502, 689
BccI CCATC 3 cut(s) 54, 85, 1027
BciT130I CCWGG 1 cut(s) 904
BciVI GTATCC 2 cut(s) 1061, 1108
BcnI CCSGG 1 cut(s) 443
BfaI CTAG 2 cut(s) 674, 1207
BfmI CTRYAG 2 cut(s) 369, 567
BfuI GTATCC 2 cut(s) 1061, 1108
BglI GCCNNNNNGGC 1 cut(s) 1017
BisI GCNGC 4 cut(s) 489, 516, 622, 678
BlsI GCNGC 4 cut(s) 490, 517, 623, 679
Bme1390I CCNGG 2 cut(s) 443, 904
BmeRI GACNNNNNGTC 2 cut(s) 142, 535
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 2 cut(s) 595, 1150
BmrFI CCNGG 2 cut(s) 443, 904
BmsI GCATC 2 cut(s) 225, 1092
BpmI CTGGAG 1 cut(s) 423
BpuMI CCSGG 1 cut(s) 443
BsaJI CCNNGG 6 cut(s) 151, 251, 442, 902, 1012, 1181
BsaWI WCCGGW 1 cut(s) 476
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bsc4I CCNNNNNNNGG 3 cut(s) 91, 441, 935
Bse118I RCCGGY 1 cut(s) 476
Bse1I ACTGG 1 cut(s) 406
BseBI CCWGG 1 cut(s) 904
BseDI CCNNGG 6 cut(s) 151, 251, 442, 902, 1012, 1181
BseGI GGATG 1 cut(s) 1083
BseLI CCNNNNNNNGG 3 cut(s) 91, 441, 935
BseNI ACTGG 1 cut(s) 406
BseXI GCAGC 3 cut(s) 500, 502, 689
BsgI GTGCAG 2 cut(s) 294, 507
BshFI GGCC 8 cut(s) 33, 123, 228, 260, 459, 1011, 1180, 1186
BshTI ACCGGT 1 cut(s) 476
BsiHKAI GWGCWC 3 cut(s) 409, 636, 762
BsiSI CCGG 4 cut(s) 102, 225, 442, 477
BslI CCNNNNNNNGG 3 cut(s) 91, 441, 935
BsnI GGCC 8 cut(s) 33, 123, 228, 260, 459, 1011, 1180, 1186
Bsp1286I GDGCHC 3 cut(s) 409, 636, 762
Bsp1407I TGTACA 1 cut(s) 360
Bsp143I GATC 1 cut(s) 603
BspACI CCGC 3 cut(s) 248, 622, 1137
BspANI GGCC 8 cut(s) 33, 123, 228, 260, 459, 1011, 1180, 1186
BspHI TCATGA 2 cut(s) 300, 606
BspLI GGNNCC 2 cut(s) 595, 1150
BspPI GGATC 1 cut(s) 611
BspQI GCTCTTC 1 cut(s) 812
BsrFI RCCGGY 1 cut(s) 476
BsrGI TGTACA 1 cut(s) 360
BsrI ACTGG 1 cut(s) 406
BssAI RCCGGY 1 cut(s) 476
BssECI CCNNGG 6 cut(s) 151, 251, 442, 902, 1012, 1181
BssMI GATC 1 cut(s) 603
BssT1I CCWWGG 3 cut(s) 151, 251, 1012
Bst2UI CCWGG 1 cut(s) 904
Bst4CI ACNGT 4 cut(s) 754, 867, 880, 993
Bst6I CTCTTC 1 cut(s) 812
BstAUI TGTACA 1 cut(s) 360
BstC8I GCNNGC 4 cut(s) 109, 312, 824, 960
BstDEI CTNAG 1 cut(s) 199
BstF5I GGATG 1 cut(s) 1083
BstHHI GCGC 1 cut(s) 12
BstKTI GATC 1 cut(s) 606
BstMBI GATC 1 cut(s) 603
BstMWI GCNNNNNNNGC 5 cut(s) 213, 497, 677, 766, 1017
BstNI CCWGG 1 cut(s) 904
BstNSI RCATGY 2 cut(s) 118, 355
BstSCI CCNGG 2 cut(s) 441, 902
BstSFI CTRYAG 2 cut(s) 369, 567
BstV1I GCAGC 3 cut(s) 500, 502, 689
BstXI CCANNNNNNTGG 1 cut(s) 345
BsuI GTATCC 2 cut(s) 1061, 1108
BsuRI GGCC 8 cut(s) 33, 123, 228, 260, 459, 1011, 1180, 1186
BtgZI GCGATG 1 cut(s) 540
BtsCI GGATG 1 cut(s) 1083
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 2 cut(s) 96, 399
Cac8I GCNNGC 4 cut(s) 109, 312, 824, 960
CciI TCATGA 2 cut(s) 300, 606
CfoI GCGC 1 cut(s) 12
Cfr10I RCCGGY 1 cut(s) 476
Cfr13I GGNCC 1 cut(s) 31
Csp6I GTAC 2 cut(s) 361, 1171
CspAI ACCGGT 1 cut(s) 476
CviAII CATG 8 cut(s) 25, 67, 115, 301, 352, 512, 607, 1030
CviQI GTAC 2 cut(s) 361, 1171
DdeI CTNAG 1 cut(s) 199
DpnI GATC 1 cut(s) 605
DpnII GATC 1 cut(s) 603
DraIII CACNNNGTG 1 cut(s) 785
DriI GACNNNNNGTC 2 cut(s) 142, 535
Eam1104I CTCTTC 1 cut(s) 812
Eam1105I GACNNNNNGTC 2 cut(s) 142, 535
EarI CTCTTC 1 cut(s) 812
Ecl136II GAGCTC 2 cut(s) 407, 634
Eco130I CCWWGG 3 cut(s) 151, 251, 1012
Eco147I AGGCCT 1 cut(s) 459
Eco24I GRGCYC 2 cut(s) 409, 636
Eco53kI GAGCTC 2 cut(s) 407, 634
EcoICRI GAGCTC 2 cut(s) 407, 634
EcoO109I RGGNCCY 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 902
EcoT14I CCWWGG 3 cut(s) 151, 251, 1012
EcoT38I GRGCYC 2 cut(s) 409, 636
ErhI CCWWGG 3 cut(s) 151, 251, 1012
FaeI CATG 8 cut(s) 28, 70, 118, 304, 355, 515, 610, 1033
FatI CATG 8 cut(s) 24, 66, 114, 300, 351, 511, 606, 1029
FauNDI CATATG 1 cut(s) 1112
Fnu4HI GCNGC 4 cut(s) 489, 516, 622, 678
FokI GGATG 1 cut(s) 1070
FriOI GRGCYC 2 cut(s) 409, 636
Fsp4HI GCNGC 4 cut(s) 489, 516, 622, 678
FspBI CTAG 2 cut(s) 674, 1207
FspI TGCGCA 1 cut(s) 11
GlaI GCGC 1 cut(s) 11
GluI GCNGC 4 cut(s) 489, 516, 622, 678
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 8 cut(s) 33, 123, 228, 260, 459, 1011, 1180, 1186
HapII CCGG 4 cut(s) 102, 225, 442, 477
HhaI GCGC 1 cut(s) 12
Hin1II CATG 8 cut(s) 28, 70, 118, 304, 355, 515, 610, 1033
Hin6I GCGC 1 cut(s) 10
HinP1I GCGC 1 cut(s) 10
HindIII AAGCTT 1 cut(s) 639
HinfI GANTC 1 cut(s) 830
HpaII CCGG 4 cut(s) 102, 225, 442, 477
HphI GGTGA 4 cut(s) 385, 406, 891, 950
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 4 cut(s) 293, 603, 1088, 1153
Hpy188III TCNNGA 3 cut(s) 301, 607, 814
Hpy8I GTNNAC 1 cut(s) 22
HpyAV CCTTC 3 cut(s) 22, 550, 604
HpyCH4III ACNGT 4 cut(s) 754, 867, 880, 993
HpyCH4V TGCA 5 cut(s) 275, 488, 500, 749, 958
HpyF10VI GCNNNNNNNGC 5 cut(s) 213, 497, 677, 766, 1017
HpyF3I CTNAG 1 cut(s) 199
Hsp92II CATG 8 cut(s) 28, 70, 118, 304, 355, 515, 610, 1033
HspAI GCGC 1 cut(s) 10
Kzo9I GATC 1 cut(s) 603
LguI GCTCTTC 1 cut(s) 812
LmnI GCTCC 3 cut(s) 104, 404, 1154
Lsp1109I GCAGC 3 cut(s) 500, 502, 689
LweI GCATC 2 cut(s) 225, 1092
MaeI CTAG 2 cut(s) 674, 1207
MaeIII GTNAC 4 cut(s) 94, 241, 316, 412
MalI GATC 1 cut(s) 605
MboI GATC 1 cut(s) 603
MboII GAAGA 5 cut(s) 69, 181, 812, 829, 1150
MhlI GDGCHC 3 cut(s) 409, 636, 762
MluCI AATT 6 cut(s) 379, 649, 696, 792, 933, 1117
MmeI TCCRAC 3 cut(s) 156, 721, 881
MnlI CCTC 9 cut(s) 59, 134, 197, 232, 862, 934, 1010, 1191, 1197
MseI TTAA 3 cut(s) 179, 423, 723
MslI CAYNNNNRTG 2 cut(s) 356, 977
MspA1I CMGCKG 2 cut(s) 491, 1139
MspI CCGG 4 cut(s) 102, 225, 442, 477
MspR9I CCNGG 2 cut(s) 443, 904
MvaI CCWGG 1 cut(s) 904
MwoI GCNNNNNNNGC 5 cut(s) 213, 497, 677, 766, 1017
NciI CCSGG 1 cut(s) 443
NdeI CATATG 1 cut(s) 1112
NdeII GATC 1 cut(s) 603
NlaIII CATG 8 cut(s) 28, 70, 118, 304, 355, 515, 610, 1033
NlaIV GGNNCC 2 cut(s) 595, 1150
NmeAIII GCCGAG 1 cut(s) 1162
NmuCI GTSAC 3 cut(s) 94, 241, 412
NsbI TGCGCA 1 cut(s) 11
NspI RCATGY 2 cut(s) 118, 355
PagI TCATGA 2 cut(s) 300, 606
PceI AGGCCT 1 cut(s) 459
PciI ACATGT 2 cut(s) 114, 351
PciSI GCTCTTC 1 cut(s) 812
PcsI WCGNNNNNNNCGW 1 cut(s) 1084
PfeI GAWTC 1 cut(s) 830
PflMI CCANNNNNTGG 1 cut(s) 935
PinAI ACCGGT 1 cut(s) 476
PkrI GCNGC 4 cut(s) 490, 517, 623, 679
PscI ACATGT 2 cut(s) 114, 351
PsiI TTATAA 1 cut(s) 695
Psp124BI GAGCTC 2 cut(s) 409, 636
Psp6I CCWGG 1 cut(s) 902
PspGI CCWGG 1 cut(s) 902
PspN4I GGNNCC 2 cut(s) 595, 1150
PspPI GGNCC 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 2 cut(s) 362, 1172
RsaNI GTAC 2 cut(s) 361, 1171
RseI CAYNNNNRTG 2 cut(s) 356, 977
SacI GAGCTC 2 cut(s) 409, 636
SapI GCTCTTC 1 cut(s) 812
SaqAI TTAA 3 cut(s) 179, 423, 723
SatI GCNGC 4 cut(s) 489, 516, 622, 678
Sau3AI GATC 1 cut(s) 603
Sau96I GGNCC 1 cut(s) 31
ScrFI CCNGG 2 cut(s) 443, 904
SduI GDGCHC 3 cut(s) 409, 636, 762
SfaNI GCATC 2 cut(s) 225, 1092
SfcI CTRYAG 2 cut(s) 369, 567
SmiMI CAYNNNNRTG 2 cut(s) 356, 977
Sse9I AATT 6 cut(s) 379, 649, 696, 792, 933, 1117
SseBI AGGCCT 1 cut(s) 459
SsiI CCGC 3 cut(s) 248, 622, 1137
SspI AATATT 1 cut(s) 916
SspMI CTAG 2 cut(s) 674, 1207
SstI GAGCTC 2 cut(s) 409, 636
StuI AGGCCT 1 cut(s) 459
StyD4I CCNGG 2 cut(s) 441, 902
StyI CCWWGG 3 cut(s) 151, 251, 1012
TaaI ACNGT 4 cut(s) 754, 867, 880, 993
TasI AATT 6 cut(s) 379, 649, 696, 792, 933, 1117
TatI WGTACW 2 cut(s) 360, 1170
TauI GCSGC 1 cut(s) 624
TfiI GAWTC 1 cut(s) 830
Tru1I TTAA 3 cut(s) 179, 423, 723
Tru9I TTAA 3 cut(s) 179, 423, 723
TscAI CASTG 2 cut(s) 103, 406
TseFI GTSAC 3 cut(s) 94, 241, 412
TseI GCWGC 3 cut(s) 488, 515, 677
Tsp45I GTSAC 3 cut(s) 94, 241, 412
TspDTI ATGAA 6 cut(s) 41, 69, 289, 577, 623, 1150
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 2 cut(s) 103, 406
Van91I CCANNNNNTGG 1 cut(s) 935
XapI RAATTY 1 cut(s) 379
XceI RCATGY 2 cut(s) 118, 355
XcmI CCANNNNNNNNNTGG 1 cut(s) 518
XspI CTAG 2 cut(s) 674, 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.