Rh1DG298500

Nodulin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0016921
Physical Location & Seq
Reverse (-)
52388363 .. 52390585
2223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG298500.1

Sequence Viewer

Length: 1605 bp
ATGGGAGCTGCTGGTATAGAAAATTGGGTGGACATGAAGAGCCTTGTCCTGCAAGTCCTGAAAGGACGATGGCTCATGGTATTTGCTTCATTTCTGATGATGGTCTCAGCCGGTGCCAGCTACATGTTCGGCCTCTACTCCAACGACATCAAGTCCGTCCTCGGCTACGACCAGAGCACACTCAACCTCATCAGCTTCTCCAAGGACTTAGGTGCCAACATCGGCGTCCTCTCCGGCCTCATCAACGAGGTCACACCCCCCTGGGTGGTCATATCCATCGGTGCAGTCCTCAATTTCTTCGGACACTTCATGATTTGGCTAGCAATAGCTAGAAAAATCCCTAAACCCCAAGTTTGGCACATGTGCATGTACATGGGTATAGGGGCTAATTCGCACACTTTTACCAACACCGGAGCTCTGGTCACCTGCGTGAAGAACTTCCCGGAGAGCCGTGGCATCGTGCTAGGGCTTTTGAAGAGCTATACTGGTATAAGTGCAGCTGTGGTGGCACAGCTGTACCATGCTGTCTATGGTGACGATACCAAGTATTTCACTTTAGTTGTGGCTTGGCTTCCGACGGCTATTTCTTTTGCTTTCATCGGAACTATTCGGATCATGAAGGTTAGTCGAAGTGGCTCAAACGAACTCAAGGCTTTTTATCATTTCCTTTATTTTTCACTTGGTCTAGCTGCATTTTTGTTGGTTATAATTATAGTGGAAAAAAACGTCAGCTTTAATCCAAGTGAGTATGTTGGAAGTGCAGCTATAGTCCTCTTTTTACTCTTTTTACCTCTTACAGTGGTAATTATGGAAGAGTATAAGGTTTGGAAGAGCAAGAGATCAATAGTAACCAAAGCCCCGACACATTCTAGCCGTGCCGAACCTGAGACTTTGGGGCACCAAGATGAACCGTTGGACCATTTTGTTGTTGTTGAAAAAGAAGTTTCTTGGCGGAAAGACGTATTTAACCCGCCGGATATCGGGGAGGACTTCACCATACTCCAAGCCGTTTTCAGCGTCGAGATGGTGACTTTACTCTTTGCAACAATTTGTGGCCTTGGAGGCACCATGACCATGATGGACAACTTGGGGCAAATTGGTACATCTTTTGGGTACCCTCTGAAAAGCATAAGAAACTTTGTGTCCCTAACAAGCATATGGAACTATTCGGGCCACATTGCTGCTGGTATAGGCTCAGAAATATTTATCACAAAGTACAAATGGCCTCGCCCTCTAATTTTCACAGCAATTCTTTTGCTCTCATGTGTTGGTCATCTTCTAATAGCATTCAACGTCCCATATGGTCTCTATGTTGCTTCAGTGGTTACTGGGTTTTGTTTTGGTGCTCATTGGCCTTTGATCTACACCATTATCTCCGAGCTTTTCGGCCTCAAGTACTACTCGACGTTGTACAATATTGGTGGATTGGCTAGCCCTATTGGGTTGTATCTGCTCAATGTGAGGGTTACTGGGTATTTATATGATGTGGAGGCTAAGAAGCAAATGGCAGCTTTGGGGCTTCAGAGGAAGGTTGGGGAAGAGTTGAACTGTGTTGGGGGGGAGTGTTTCAAATTGTCTTTCATCATCATTACTGTGGTTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 Nodulin-like

Protein Analysis

534

Amino Acids

58.93

Weight (kDa)

8.48

Isoelectric Point (pI)

26.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nodulin-like PF06813 23 - 269 2e-85 Nodulin-like
NFD4_C PF23262 354 - 530 9.7e-09 NFD4, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016921)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g18450
malus_domestica MD01G1101200.v1.1
prunus_persica Prupe.2G206400_v2.0.a1
pyrus_communis pycom01g12780
rosa_chinensis RchiOBHm_Chr1g0362251
rosa_laevigata RLG00000027663
rosa_multiflora Rmu_sc0001237.1_g000013 Rmu_ssc0000009.1_g000001
rosa_roxburghii Rroxscaffold_4G00294400
rosa_rugosa Rorug01G0296000
rosa_samantha Rh1AG305400 Rh1BG268400 Rh1CG286500 Rh1DG298500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 707
AarI CACCTGC 1 cut(s) 434
Acc36I ACCTGC 1 cut(s) 434
Acc65I GGTACC 1 cut(s) 1113
AccB1I GGYRCC 5 cut(s) 113, 212, 897, 1064, 1113
AciI CCGC 2 cut(s) 952, 971
AclWI GGATC 1 cut(s) 620
AcuI CTGAAG 2 cut(s) 1302, 1505
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 7 cut(s) 371, 518, 1102, 1115, 1217, 1397, 1412
AfiI CCNNNNNNNGG 3 cut(s) 265, 354, 980
AflIII ACRYGT 2 cut(s) 123, 360
AgsI TTSAA 5 cut(s) 475, 935, 1291, 1546, 1570
AhdI GACNNNNNGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 260
AleI CACNNNNGTG 1 cut(s) 428
Alw21I GWGCWC 3 cut(s) 179, 418, 1348
Alw26I GTCTC 3 cut(s) 109, 881, 1310
AlwI GGATC 1 cut(s) 620
AoxI GGCC 7 cut(s) 130, 235, 1054, 1171, 1223, 1352, 1387
ApeKI GCWGC 6 cut(s) 8, 497, 689, 761, 1181, 1508
Asp700I GAANNNNTTC 1 cut(s) 437
Asp718I GGTACC 1 cut(s) 1113
AspS9I GGNCC 2 cut(s) 916, 1171
AsuC2I CCSGG 1 cut(s) 443
AsuHPI GGTGA 4 cut(s) 415, 545, 985, 1039
AsuNHI GCTAGC 2 cut(s) 319, 1430
AvaII GGWCC 1 cut(s) 916
BaeGI GKGCMC 1 cut(s) 900
BaeI ACNNNNGTAYC 2 cut(s) 500, 533
BanI GGYRCC 5 cut(s) 113, 212, 897, 1064, 1113
BanII GRGCYC 1 cut(s) 418
Bbv12I GWGCWC 3 cut(s) 179, 418, 1348
BbvI GCAGC 5 cut(s) 509, 676, 773, 1168, 1520
BccI CCATC 5 cut(s) 63, 94, 284, 1018, 1072
BceAI ACGGC 4 cut(s) 435, 594, 858, 992
BciT130I CCWGG 1 cut(s) 262
BcnI CCSGG 1 cut(s) 443
BcoDI GTCTC 3 cut(s) 109, 881, 1310
BfaI CTAG 6 cut(s) 320, 330, 464, 686, 870, 1431
BfmI CTRYAG 1 cut(s) 765
BfuAI ACCTGC 1 cut(s) 434
BglI GCCNNNNNGGC 1 cut(s) 1062
BisI GCNGC 6 cut(s) 9, 498, 690, 762, 1182, 1509
BlsI GCNGC 6 cut(s) 10, 499, 691, 763, 1183, 1510
BmcAI AGTACT 1 cut(s) 1397
Bme1390I CCNGG 2 cut(s) 262, 443
Bme18I GGWCC 1 cut(s) 916
BmeRI GACNNNNNGTC 1 cut(s) 151
BmgT120I GGNCC 2 cut(s) 916, 1171
BmiI GGNNCC 5 cut(s) 115, 214, 899, 1066, 1115
BmrFI CCNGG 2 cut(s) 262, 443
BmrI ACTGGG 2 cut(s) 1338, 1479
BmsI GCATC 1 cut(s) 465
BmtI GCTAGC 2 cut(s) 323, 1434
BmuI ACTGGG 2 cut(s) 1338, 1479
BpuEI CTTGAG 2 cut(s) 632, 1376
BpuMI CCSGG 1 cut(s) 443
BsaHI GRCGYC 1 cut(s) 225
BsaI GGTCTC 2 cut(s) 109, 1310
BsaJI CCNNGG 6 cut(s) 160, 201, 260, 261, 451, 1057
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 405, 435
Bsc4I CCNNNNNNNGG 3 cut(s) 265, 354, 980
Bse118I RCCGGY 1 cut(s) 110
Bse1I ACTGG 3 cut(s) 490, 1333, 1474
Bse3DI GCAATG 1 cut(s) 1176
BseBI CCWGG 1 cut(s) 262
BseDI CCNNGG 6 cut(s) 160, 201, 260, 261, 451, 1057
BseLI CCNNNNNNNGG 3 cut(s) 265, 354, 980
BseMI GCAATG 1 cut(s) 1176
BseMII CTCAG 3 cut(s) 120, 876, 1209
BseNI ACTGG 3 cut(s) 490, 1333, 1474
BseSI GKGCMC 1 cut(s) 900
BseXI GCAGC 5 cut(s) 509, 676, 773, 1168, 1520
BsgI GTGCAG 3 cut(s) 303, 516, 780
BshFI GGCC 7 cut(s) 132, 237, 1056, 1173, 1225, 1354, 1389
BshNI GGYRCC 5 cut(s) 113, 212, 897, 1064, 1113
BsiHKAI GWGCWC 3 cut(s) 179, 418, 1348
BsiSI CCGG 5 cut(s) 111, 234, 411, 443, 974
BslFI GGGAC 2 cut(s) 1129, 1280
BslI CCNNNNNNNGG 3 cut(s) 265, 354, 980
BsmAI GTCTC 3 cut(s) 109, 881, 1310
BsmFI GGGAC 2 cut(s) 1129, 1280
BsmI GAATGC 1 cut(s) 1286
BsnI GGCC 7 cut(s) 132, 237, 1056, 1173, 1225, 1354, 1389
Bso31I GGTCTC 2 cut(s) 109, 1310
Bsp1286I GDGCHC 4 cut(s) 179, 418, 900, 1348
Bsp1407I TGTACA 2 cut(s) 369, 1410
Bsp143I GATC 3 cut(s) 612, 839, 1359
BspACI CCGC 2 cut(s) 952, 971
BspANI GGCC 7 cut(s) 132, 237, 1056, 1173, 1225, 1354, 1389
BspCNI CTCAG 3 cut(s) 119, 877, 1208
BspHI TCATGA 2 cut(s) 309, 615
BspLI GGNNCC 5 cut(s) 115, 214, 899, 1066, 1115
BspMI ACCTGC 1 cut(s) 434
BspOI GCTAGC 2 cut(s) 323, 1434
BspPI GGATC 1 cut(s) 620
BspQI GCTCTTC 3 cut(s) 32, 470, 824
BspT107I GGYRCC 5 cut(s) 113, 212, 897, 1064, 1113
BspTNI GGTCTC 2 cut(s) 109, 1310
BsrDI GCAATG 1 cut(s) 1176
BsrFI RCCGGY 1 cut(s) 110
BsrGI TGTACA 2 cut(s) 369, 1410
BsrI ACTGG 3 cut(s) 490, 1333, 1474
BssAI RCCGGY 1 cut(s) 110
BssECI CCNNGG 6 cut(s) 160, 201, 260, 261, 451, 1057
BssMI GATC 3 cut(s) 612, 839, 1359
BssNI GRCGYC 1 cut(s) 225
BssT1I CCWWGG 2 cut(s) 201, 1057
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 4 cut(s) 799, 912, 1550, 1594
Bst6I CTCTTC 5 cut(s) 32, 470, 807, 824, 1533
BstACI GRCGYC 1 cut(s) 225
BstAUI TGTACA 2 cut(s) 369, 1410
BstC8I GCNNGC 3 cut(s) 118, 321, 1432
BstDEI CTNAG 5 cut(s) 106, 208, 885, 1195, 1494
BstDSI CCRYGG 1 cut(s) 451
BstEII GGTNACC 1 cut(s) 421
BstKTI GATC 3 cut(s) 615, 842, 1362
BstMAI GTCTC 3 cut(s) 109, 881, 1310
BstMBI GATC 3 cut(s) 612, 839, 1359
BstMWI GCNNNNNNNGC 2 cut(s) 506, 1062
BstNI CCWGG 1 cut(s) 262
BstNSI RCATGY 3 cut(s) 127, 364, 370
BstPI GGTNACC 1 cut(s) 421
BstSCI CCNGG 2 cut(s) 260, 441
BstSFI CTRYAG 1 cut(s) 765
BstSLI GKGCMC 1 cut(s) 900
BstV1I GCAGC 5 cut(s) 509, 676, 773, 1168, 1520
BsuRI GGCC 7 cut(s) 132, 237, 1056, 1173, 1225, 1354, 1389
BtgI CCRYGG 1 cut(s) 451
BtsIMutI CAGTG 2 cut(s) 804, 1326
BveI ACCTGC 1 cut(s) 434
Cac8I GCNNGC 3 cut(s) 118, 321, 1432
CciI TCATGA 2 cut(s) 309, 615
Cfr10I RCCGGY 1 cut(s) 110
Cfr13I GGNCC 2 cut(s) 916, 1171
CseI GACGC 2 cut(s) 214, 1006
Csp6I GTAC 7 cut(s) 370, 517, 1101, 1114, 1216, 1396, 1411
CspCI CAANNNNNGTGG 2 cut(s) 1402, 1437
CviQI GTAC 7 cut(s) 370, 517, 1101, 1114, 1216, 1396, 1411
DdeI CTNAG 5 cut(s) 106, 208, 885, 1195, 1494
DpnI GATC 3 cut(s) 614, 841, 1361
DpnII GATC 3 cut(s) 612, 839, 1359
DriI GACNNNNNGTC 1 cut(s) 151
Eam1104I CTCTTC 5 cut(s) 32, 470, 807, 824, 1533
Eam1105I GACNNNNNGTC 1 cut(s) 151
EarI CTCTTC 5 cut(s) 32, 470, 807, 824, 1533
EciI GGCGGA 1 cut(s) 967
Ecl136II GAGCTC 1 cut(s) 416
Eco130I CCWWGG 2 cut(s) 201, 1057
Eco24I GRGCYC 1 cut(s) 418
Eco31I GGTCTC 2 cut(s) 109, 1310
Eco32I GATATC 1 cut(s) 979
Eco47I GGWCC 1 cut(s) 916
Eco53kI GAGCTC 1 cut(s) 416
Eco57I CTGAAG 2 cut(s) 1302, 1505
Eco91I GGTNACC 1 cut(s) 421
EcoICRI GAGCTC 1 cut(s) 416
EcoO65I GGTNACC 1 cut(s) 421
EcoRII CCWGG 1 cut(s) 260
EcoRV GATATC 1 cut(s) 979
EcoT14I CCWWGG 2 cut(s) 201, 1057
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 2 cut(s) 201, 1057
FaqI GGGAC 2 cut(s) 1129, 1280
FauI CCCGC 1 cut(s) 978
FauNDI CATATG 2 cut(s) 1157, 1300
Fnu4HI GCNGC 6 cut(s) 9, 498, 690, 762, 1182, 1509
FriOI GRGCYC 1 cut(s) 418
Fsp4HI GCNGC 6 cut(s) 9, 498, 690, 762, 1182, 1509
FspBI CTAG 6 cut(s) 320, 330, 464, 686, 870, 1431
GluI GCNGC 6 cut(s) 9, 498, 690, 762, 1182, 1509
HaeIII GGCC 7 cut(s) 132, 237, 1056, 1173, 1225, 1354, 1389
HapII CCGG 5 cut(s) 111, 234, 411, 443, 974
HgaI GACGC 2 cut(s) 214, 1006
Hin1I GRCGYC 1 cut(s) 225
HpaII CCGG 5 cut(s) 111, 234, 411, 443, 974
HphI GGTGA 4 cut(s) 415, 545, 985, 1039
Hpy166II GTNNAC 1 cut(s) 31
Hpy188I TCNGA 9 cut(s) 96, 302, 576, 602, 612, 1122, 1198, 1378, 1524
Hpy188III TCNNGA 4 cut(s) 58, 310, 616, 1021
Hpy8I GTNNAC 1 cut(s) 31
Hpy99I CGWCG 3 cut(s) 580, 1022, 1408
HpyAV CCTTC 2 cut(s) 613, 1522
HpyCH4III ACNGT 4 cut(s) 799, 912, 1550, 1594
HpyCH4IV ACGT 4 cut(s) 726, 960, 1293, 1406
HpyCH4V TGCA 7 cut(s) 52, 284, 366, 497, 692, 761, 1043
HpyF10VI GCNNNNNNNGC 2 cut(s) 506, 1062
HpyF3I CTNAG 5 cut(s) 106, 208, 885, 1195, 1494
HpySE526I ACGT 4 cut(s) 726, 960, 1293, 1406
Hsp92I GRCGYC 1 cut(s) 225
KpnI GGTACC 1 cut(s) 1117
Kzo9I GATC 3 cut(s) 612, 839, 1359
LguI GCTCTTC 3 cut(s) 32, 470, 824
LmnI GCTCC 2 cut(s) 5, 413
Lsp1109I GCAGC 5 cut(s) 509, 676, 773, 1168, 1520
LweI GCATC 1 cut(s) 465
MaeI CTAG 6 cut(s) 320, 330, 464, 686, 870, 1431
MaeII ACGT 4 cut(s) 726, 960, 1293, 1406
MaeIII GTNAC 7 cut(s) 250, 421, 533, 847, 1027, 1324, 1465
MalI GATC 3 cut(s) 614, 841, 1361
MboI GATC 3 cut(s) 612, 839, 1359
MboII GAAGA 8 cut(s) 49, 289, 445, 487, 824, 841, 1268, 1550
MhlI GDGCHC 4 cut(s) 179, 418, 900, 1348
MmeI TCCRAC 4 cut(s) 165, 599, 733, 894
MroXI GAANNNNTTC 1 cut(s) 437
MseI TTAA 2 cut(s) 735, 966
MslI CAYNNNNRTG 6 cut(s) 365, 371, 428, 903, 1073, 1592
MspA1I CMGCKG 2 cut(s) 500, 514
MspI CCGG 5 cut(s) 111, 234, 411, 443, 974
MspR9I CCNGG 2 cut(s) 262, 443
Mva1269I GAATGC 1 cut(s) 1286
MvaI CCWGG 1 cut(s) 262
MwoI GCNNNNNNNGC 2 cut(s) 506, 1062
NciI CCSGG 1 cut(s) 443
NdeI CATATG 2 cut(s) 1157, 1300
NdeII GATC 3 cut(s) 612, 839, 1359
NheI GCTAGC 2 cut(s) 319, 1430
NlaIV GGNNCC 5 cut(s) 115, 214, 899, 1066, 1115
NmeAIII GCCGAG 1 cut(s) 141
NmuCI GTSAC 4 cut(s) 250, 421, 533, 1027
NspI RCATGY 3 cut(s) 127, 364, 370
OliI CACNNNNGTG 1 cut(s) 428
PagI TCATGA 2 cut(s) 309, 615
PaqCI CACCTGC 1 cut(s) 434
PasI CCCWGGG 1 cut(s) 261
PciI ACATGT 2 cut(s) 123, 360
PciSI GCTCTTC 3 cut(s) 32, 470, 824
PctI GAATGC 1 cut(s) 1286
PdmI GAANNNNTTC 1 cut(s) 437
PfoI TCCNGGA 1 cut(s) 441
PkrI GCNGC 6 cut(s) 10, 499, 691, 763, 1183, 1510
PscI ACATGT 2 cut(s) 123, 360
PsiI TTATAA 1 cut(s) 707
Psp124BI GAGCTC 1 cut(s) 418
Psp6I CCWGG 1 cut(s) 260
PspEI GGTNACC 1 cut(s) 421
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 5 cut(s) 115, 214, 899, 1066, 1115
PspPI GGNCC 2 cut(s) 916, 1171
PvuII CAGCTG 2 cut(s) 500, 514
RsaI GTAC 7 cut(s) 371, 518, 1102, 1115, 1217, 1397, 1412
RsaNI GTAC 7 cut(s) 370, 517, 1101, 1114, 1216, 1396, 1411
RseI CAYNNNNRTG 6 cut(s) 365, 371, 428, 903, 1073, 1592
SacI GAGCTC 1 cut(s) 418
SapI GCTCTTC 3 cut(s) 32, 470, 824
SaqAI TTAA 2 cut(s) 735, 966
SatI GCNGC 6 cut(s) 9, 498, 690, 762, 1182, 1509
Sau3AI GATC 3 cut(s) 612, 839, 1359
Sau96I GGNCC 2 cut(s) 916, 1171
ScaI AGTACT 1 cut(s) 1397
ScrFI CCNGG 2 cut(s) 262, 443
SduI GDGCHC 4 cut(s) 179, 418, 900, 1348
SfaNI GCATC 1 cut(s) 465
SfcI CTRYAG 1 cut(s) 765
SinI GGWCC 1 cut(s) 916
SmiMI CAYNNNNRTG 6 cut(s) 365, 371, 428, 903, 1073, 1592
SmlI CTYRAG 2 cut(s) 647, 1391
SmoI CTYRAG 2 cut(s) 647, 1391
SsiI CCGC 2 cut(s) 952, 971
SspI AATATT 2 cut(s) 1203, 1417
SspMI CTAG 6 cut(s) 320, 330, 464, 686, 870, 1431
SstI GAGCTC 1 cut(s) 418
StyD4I CCNGG 2 cut(s) 260, 441
StyI CCWWGG 2 cut(s) 201, 1057
TaaI ACNGT 4 cut(s) 799, 912, 1550, 1594
TaiI ACGT 4 cut(s) 729, 963, 1296, 1409
TaqI TCGA 3 cut(s) 628, 1020, 1403
TatI WGTACW 4 cut(s) 369, 1215, 1395, 1410
Tru1I TTAA 2 cut(s) 735, 966
Tru9I TTAA 2 cut(s) 735, 966
TscAI CASTG 2 cut(s) 804, 1326
TseFI GTSAC 4 cut(s) 250, 421, 533, 1027
TseI GCWGC 6 cut(s) 8, 497, 689, 761, 1181, 1508
Tsp45I GTSAC 4 cut(s) 250, 421, 533, 1027
TspDTI ATGAA 7 cut(s) 50, 78, 298, 586, 632, 921, 1570
TspGWI ACGGA 1 cut(s) 145
TspRI CASTG 2 cut(s) 804, 1326
VpaK11BI GGWCC 1 cut(s) 916
XceI RCATGY 3 cut(s) 127, 364, 370
XcmI CCANNNNNNNNNTGG 3 cut(s) 527, 1075, 1181
XmnI GAANNNNTTC 1 cut(s) 437
XspI CTAG 6 cut(s) 320, 330, 464, 686, 870, 1431
ZrmI AGTACT 1 cut(s) 1397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.