FvH4_7g19000

DNL-type zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015054
Physical Location & Seq
Reverse (-)
15787258 .. 15789788
2531 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g19000.t1

Sequence Viewer

Length: 756 bp
ATGGCCGCTCGAATATTGCAGAAGCGAATCGCCTCACTCCTCTCCCCCAAGCAGCCTTCTCTCTCCCAAGTTTTAAAAGAACAATCTGTTCCTTCTGTGTCGTCTATCTTCAGCAGATTTCAGCTTGGGGAAAGAGATATTCAGTCTGAAACAAAGCCAGCTGATCTTACCAATGAGGATTTCAAGTTTTCTGATCTTGCAAATGAGGATCCTAAGATTGAAAATCACAAAGCTGATGGTTTGAAGCCTAGTTATGACTCAGATGATCAACTATCTGATTCAGATTTTAAGTTTGCTTCTCTTGCAGATGAGGATCCTAAGATTGAAAATCACGAAGCTGATGGTTTGAAGCCTAGTTGTGACTCAGATGATCAACAATCTGAGTCATCTCCTGGCAAACTCGTTGATATCAAAATGCACGCTGAATACAACTTGAAATTATCCCCGAGGCATGACCTTTGTTTGATCTTCACTTGCACAGTCTGTGACACAAGAACAATGAAGACAGCTTCTAAAGAAGCATACGAGAAAGGTGTGGTGGTGGCACGCTGTAGTGGGTGCAACAACCTCCACCTGATGGCTGATCATCTTGGAATGTTTGGAGAGAAGAGCACCGTTGAGGACTTTCTGGCTTCCCGTGGGGAGGAGGTGAAGAAAGGTCATTCTGATACACTGAACCTGACTCTTGAAGATCTGGCTGGAAAGGATTTAGCTGCAAAGATAGTCGACAACGAAGCTTCAACTTCAGCCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-DNL

Protein Analysis

252

Amino Acids

27.56

Weight (kDa)

4.88

Isoelectric Point (pI)

45.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-DNL PF05180 155 - 215 4.2e-25 DNL zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015054)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54826
fragaria_vesca FvH4_7g19000
malus_domestica MD01G1105600.v1.1 MD07G1171800.v1.1
pyrus_communis pycom01g13240 pycom07g16640
rosa_chinensis RchiOBHm_Chr1g0362931
rosa_laevigata RLG00000027611
rosa_multiflora Rmu_sc0001502.1_g000005
rosa_roxburghii Rroxscaffold_4G00293640
rosa_rugosa Rorug01G0302200
rosa_samantha Rh1AG311700 Rh1BG274800 Rh1CG292000 Rh1DG305500
rosa_wichuraiana Rw1G027620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 577
AccBSI CCGCTC 1 cut(s) 8
AccI GTMKAC 1 cut(s) 726
AciI CCGC 1 cut(s) 6
AclWI GGATC 4 cut(s) 203, 216, 308, 321
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 2 cut(s) 94, 729
AfiI CCNNNNNNNGG 2 cut(s) 577, 643
AgsI TTSAA 8 cut(s) 184, 221, 244, 326, 349, 436, 689, 741
AjnI CCWGG 1 cut(s) 391
AjuI GAANNNNNNNTTGG 2 cut(s) 164, 196
AluBI AGCT 7 cut(s) 124, 161, 233, 338, 509, 713, 737
AluI AGCT 7 cut(s) 124, 161, 233, 338, 509, 713, 737
Alw21I GWGCWC 1 cut(s) 614
AlwI GGATC 4 cut(s) 203, 216, 308, 321
Ama87I CYCGRG 1 cut(s) 445
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 2 cut(s) 52, 713
AsuHPI GGTGA 1 cut(s) 661
AvaI CYCGRG 1 cut(s) 445
BamHI GGATCC 2 cut(s) 208, 313
BbsI GAAGAC 1 cut(s) 509
Bbv12I GWGCWC 1 cut(s) 614
BbvI GCAGC 2 cut(s) 64, 700
BccI CCATC 3 cut(s) 230, 335, 571
BciT130I CCWGG 1 cut(s) 393
BclI TGATCA 3 cut(s) 265, 370, 583
BfaI CTAG 2 cut(s) 249, 354
BfmI CTRYAG 1 cut(s) 550
BglII AGATCT 1 cut(s) 691
BisI GCNGC 3 cut(s) 6, 53, 714
BlsI GCNGC 3 cut(s) 7, 54, 715
Bme1390I CCNGG 1 cut(s) 393
BmeT110I CYCGRG 1 cut(s) 445
BmiI GGNNCC 2 cut(s) 210, 315
BmrFI CCNGG 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 509
BsaBI GATNNNNATC 1 cut(s) 312
BsaJI CCNNGG 2 cut(s) 446, 637
Bsc4I CCNNNNNNNGG 2 cut(s) 577, 643
Bse1I ACTGG 1 cut(s) 750
Bse8I GATNNNNATC 1 cut(s) 312
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 2 cut(s) 446, 637
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 2 cut(s) 577, 643
BseMII CTCAG 3 cut(s) 273, 372, 378
BseNI ACTGG 1 cut(s) 750
BseRI GAGGAG 2 cut(s) 29, 659
BseXI GCAGC 2 cut(s) 64, 700
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 614
BsiHKCI CYCGRG 1 cut(s) 445
BslI CCNNNNNNNGG 2 cut(s) 577, 643
BsnI GGCC 1 cut(s) 5
BsoBI CYCGRG 1 cut(s) 445
Bsp1286I GDGCHC 1 cut(s) 614
Bsp143I GATC 9 cut(s) 163, 193, 208, 265, 313, 370, 465, 583, 691
BspACI CCGC 1 cut(s) 6
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 272, 373, 377
BspLI GGNNCC 2 cut(s) 210, 315
BspPI GGATC 4 cut(s) 203, 216, 308, 321
BspQI GCTCTTC 1 cut(s) 602
BsrBI CCGCTC 1 cut(s) 8
BsrI ACTGG 1 cut(s) 750
BssECI CCNNGG 2 cut(s) 446, 637
BssMI GATC 9 cut(s) 163, 193, 208, 265, 313, 370, 465, 583, 691
Bst2UI CCWGG 1 cut(s) 393
Bst4CI ACNGT 2 cut(s) 481, 616
Bst6I CTCTTC 1 cut(s) 602
BstC8I GCNNGC 3 cut(s) 159, 420, 547
BstDEI CTNAG 5 cut(s) 213, 259, 318, 364, 381
BstDSI CCRYGG 1 cut(s) 637
BstKTI GATC 9 cut(s) 166, 196, 211, 268, 316, 373, 468, 586, 694
BstMBI GATC 9 cut(s) 163, 193, 208, 265, 313, 370, 465, 583, 691
BstMWI GCNNNNNNNGC 1 cut(s) 302
BstNI CCWGG 1 cut(s) 393
BstSCI CCNGG 1 cut(s) 391
BstSFI CTRYAG 1 cut(s) 550
BstV1I GCAGC 2 cut(s) 64, 700
BstV2I GAAGAC 1 cut(s) 509
BstX2I RGATCY 3 cut(s) 208, 313, 691
BstYI RGATCY 3 cut(s) 208, 313, 691
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 637
BtsIMutI CAGTG 1 cut(s) 671
Cac8I GCNNGC 3 cut(s) 159, 420, 547
CviAII CATG 1 cut(s) 452
DdeI CTNAG 5 cut(s) 213, 259, 318, 364, 381
DpnI GATC 9 cut(s) 165, 195, 210, 267, 315, 372, 467, 585, 693
DpnII GATC 9 cut(s) 163, 193, 208, 265, 313, 370, 465, 583, 691
DraI TTTAAA 1 cut(s) 75
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 602
EarI CTCTTC 1 cut(s) 602
Eco32I GATATC 1 cut(s) 409
Eco57I CTGAAG 2 cut(s) 94, 729
Eco88I CYCGRG 1 cut(s) 445
EcoRII CCWGG 1 cut(s) 391
EcoRV GATATC 1 cut(s) 409
FaeI CATG 1 cut(s) 455
FaiI YATR 3 cut(s) 255, 453, 523
FatI CATG 1 cut(s) 451
FbaI TGATCA 3 cut(s) 265, 370, 583
FblI GTMKAC 1 cut(s) 726
Fnu4HI GCNGC 3 cut(s) 6, 53, 714
Fsp4HI GCNGC 3 cut(s) 6, 53, 714
FspBI CTAG 2 cut(s) 249, 354
GluI GCNGC 3 cut(s) 6, 53, 714
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 1 cut(s) 455
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 1 cut(s) 735
HinfI GANTC 6 cut(s) 27, 257, 278, 362, 383, 682
HphI GGTGA 1 cut(s) 661
Hpy166II GTNNAC 1 cut(s) 727
Hpy188I TCNGA 8 cut(s) 148, 193, 262, 277, 283, 367, 382, 667
Hpy188III TCNNGA 2 cut(s) 332, 686
Hpy8I GTNNAC 1 cut(s) 727
HpyAV CCTTC 2 cut(s) 66, 102
HpyCH4III ACNGT 2 cut(s) 481, 616
HpyCH4V TGCA 7 cut(s) 19, 200, 305, 418, 477, 561, 716
HpyF10VI GCNNNNNNNGC 1 cut(s) 302
HpyF3I CTNAG 5 cut(s) 213, 259, 318, 364, 381
Hsp92II CATG 1 cut(s) 455
Ksp22I TGATCA 3 cut(s) 265, 370, 583
Kzo9I GATC 9 cut(s) 163, 193, 208, 265, 313, 370, 465, 583, 691
LguI GCTCTTC 1 cut(s) 602
LpnPI CCDG 8 cut(s) 171, 378, 405, 587, 614, 680, 684, 692
Lsp1109I GCAGC 2 cut(s) 64, 700
MaeI CTAG 2 cut(s) 249, 354
MaeIII GTNAC 2 cut(s) 359, 485
MalI GATC 9 cut(s) 165, 195, 210, 267, 315, 372, 467, 585, 693
MbiI CCGCTC 1 cut(s) 8
MboI GATC 9 cut(s) 163, 193, 208, 265, 313, 370, 465, 583, 691
MboII GAAGA 6 cut(s) 100, 460, 514, 619, 664, 701
MflI RGATCY 3 cut(s) 208, 313, 691
MhlI GDGCHC 1 cut(s) 614
MluCI AATT 1 cut(s) 437
MlyI GAGTC 4 cut(s) 251, 356, 392, 676
MseI TTAA 2 cut(s) 74, 288
MspA1I CMGCKG 1 cut(s) 161
MspR9I CCNGG 1 cut(s) 393
MvaI CCWGG 1 cut(s) 393
MwoI GCNNNNNNNGC 1 cut(s) 302
NdeII GATC 9 cut(s) 163, 193, 208, 265, 313, 370, 465, 583, 691
NlaIII CATG 1 cut(s) 455
NlaIV GGNNCC 2 cut(s) 210, 315
NmuCI GTSAC 2 cut(s) 359, 485
PciSI GCTCTTC 1 cut(s) 602
PfeI GAWTC 2 cut(s) 27, 278
PflMI CCANNNNNTGG 1 cut(s) 577
PkrI GCNGC 3 cut(s) 7, 54, 715
PleI GAGTC 4 cut(s) 251, 356, 391, 676
PpsI GAGTC 4 cut(s) 251, 356, 391, 676
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 2 cut(s) 210, 315
PsuI RGATCY 3 cut(s) 208, 313, 691
PvuII CAGCTG 1 cut(s) 161
SalI GTCGAC 1 cut(s) 725
SapI GCTCTTC 1 cut(s) 602
SaqAI TTAA 2 cut(s) 74, 288
SatI GCNGC 3 cut(s) 6, 53, 714
Sau3AI GATC 9 cut(s) 163, 193, 208, 265, 313, 370, 465, 583, 691
SchI GAGTC 4 cut(s) 251, 356, 392, 676
ScrFI CCNGG 1 cut(s) 393
SduI GDGCHC 1 cut(s) 614
SfcI CTRYAG 1 cut(s) 550
Sse9I AATT 1 cut(s) 437
SsiI CCGC 1 cut(s) 6
SspI AATATT 1 cut(s) 15
SspMI CTAG 2 cut(s) 249, 354
StyD4I CCNGG 1 cut(s) 391
TaaI ACNGT 2 cut(s) 481, 616
TaqI TCGA 2 cut(s) 10, 726
TasI AATT 1 cut(s) 437
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 27, 278
Tru1I TTAA 2 cut(s) 74, 288
Tru9I TTAA 2 cut(s) 74, 288
TscAI CASTG 1 cut(s) 678
TseFI GTSAC 2 cut(s) 359, 485
TseI GCWGC 2 cut(s) 52, 713
Tsp45I GTSAC 2 cut(s) 359, 485
TspDTI ATGAA 1 cut(s) 515
TspRI CASTG 1 cut(s) 678
Van91I CCANNNNNTGG 1 cut(s) 577
XmiI GTMKAC 1 cut(s) 726
XspI CTAG 2 cut(s) 249, 354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.