FvH4_7g19460

Endoplasmic reticulum metallopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0011223
Physical Location & Seq
Reverse (-)
16037031 .. 16043146
6116 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g19460.t2

Sequence Viewer

Length: 2310 bp
ATGGTGAGTGGATTGTTTAAGGGGAAGACACTTGTGTATTCGGATTTGAGTCACATTGTGGTGAGAGTCTCGCCCAAGTATGCAGCTGATTCTGTGGACAATGCTGTTCTGGTCTCTTCTCACATTGACACTGTCTTCTCAACAGGAGGGGCCGGAGATTGCAGCTCGTGTGTGGCTGTGATGTTGGAACTTGCTCGCGGAGTTTCGCAGTGGGCGCATGGATTTAAGCATGCTGTTATATTTTTGTTTAATACCGGGGAGGAGGAGGGCCTAAGTGGTGCTCATAGCTTTATAACTCAGCATCCATGGAGGAAGACAATTCGTCTGGCTATTGATTTGGAGGCCATGGGTATCGGAGGGAAGTCTGGCATATTCCAGGCTGGTCCTCTTCCTTGGGCTATTGAGAATTATGCAGCAGCAGCAAAATACCCATCTGGCCACATTATAGGACAGGATATATTTTCCTCTGGAGCCATCAAATCTGCAACAGACTTCCAAGTTTATAAAGAGTTAGCTGGCCTTTCGGGGCTTGACTTTGCTTACACAGAAAACGGTGCAGTATACCACACAAAGAATGACAAGTTTGAGCTTCTTCAATTGGGATCTCTTCAACATCTTGGAGAAAATATGCTTGCTTTTCTTCTTCGGGTTGCTGCATCCTCTTATCTTCCAAAGGCCAATGTAATGCAAGAAGAGGATAAAACAGGCCTGAGTGCTGCCATATATTTTGACATTTTGGGGTGGTACATGATTGTCTACCGACAACGCTTCGCCAGAATGCTTTATAATTCAGTGATAGCGCAATCACTTCTTATATGGACTACATCATTGCTTATGGGTGGTTATCCAGCTGCAGTTTCACTGGTCTTGTCATGTTTAAGTGTTATTCTTATGTGGACATTTGCATTAAGCTTCTCAGTTATTGTGGCCTTCATCATCCCCCTAATATCATCGTCACCAGTGCCATATATCGCAAACCCATGGCTGGTAGTTGGGTTATTCGCTGCACCTGCTCTGCTTGGAGCATTAACTGGTCAATATTTGGGTTATCTTGTTCTTCATAAGTATTTGGCAAACACTTACTCCAAGAAGAAACAGCTGTCACCTGCTATCAGAACCGATTTGGTTAAATTAGAGGCTGAGAGATGGCTCTATAAAGCTGGATCGATTCAATGGCTCATTCTCCTCAGTTTGGGAACCTATTACAGAATTGGATCATCTTATCTGGCTCTTGCTTGGTTGGTTCCGCCAGCATTCGCATATGGCTTTCTTGAAGCAACTCTAAGCCCAGCCCGATCACCTAAGCCTCTGAAACTTGCAACCCTGCTGATAGGATTAGCTATACCAGTAATACTTTCTGCTGGCGTATTCATTCGGTTGGCTGGCACAATAATTGGGGGCATGGTTCGTTTTGATAGGAATCCTGGTGGCACTCCTGATTGGCTGGGAAATGTTATACTTGCTGTTTTTGTAGCTACTGTCATGTGCCTGACTTTGGTGTATCTTCTTTCATACATTCATCTTTCAGGTGCCAAGAGATTGATTATCCTCTCAACTTGCGCCATGTTTGGCCTCTCGCTTGCTCTTGTATTATCTGGTACTGTACCAGCATTTACCAATGACACATCAAGAGCTGTAAACGTTGTGCATGTTGTAGATACTACAAGAAGCATTGAAGATCCTCGATCCTATGTGTCTCTGTTTTCTTTAACTCCGGGAAAATTGACAAAGGAGGTTGAGCAAATTAAGGAAGGTTTCAGATGTGGTCGAGATCAGGTCTTTGATTTTGTAACGTTTACAGTCAAATATGGATGTTGGACCGAGGATGACAGTGACAGTGGGTGGAGTGAAGCAGACATTCCTGTAATGCATGTCCAGAGTGATACTCAAGGAATTGAAAGAACAACAGAAGTTATAATCGACACAAAGGGTTCAATACGGTGGGCTCTTGCCATCAACACCAATGAAATACGGGATTATGCATTCACAGATGCTGGAAACTCTGAGGAGTTGGTTTCAGTTGGTGACAAGAGCAATGCAGATGGTTGGCACGTTATTCAGTTTGCTGGGGGAAACAATTCACCGAGGACGTTTGGTCTGACTCTCTTCTGGACTAAAAGCTCTACCCTTAAGGCAGACGGGAAGAGGGACGGGCAAGCTCCCCTTCTCAAGTTAAGAACTGATATGGACATTGTAACACCGAAAGTCGAAAGAGTTCTTTCAAAGCTTCCTACTTGGTGTTCACTGTTTGGCAAGTCGACGTCTCCCTACACCTTAGCATTCTTAAGTAGTCTTCCTGTTGATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M28

Protein Analysis

770

Amino Acids

83.85

Weight (kDa)

7.6

Isoelectric Point (pI)

33.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M28 PF04389 166 - 351 2.9e-45 Peptidase family M28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20660 AT5G20660 AT5G20660 AT5G20660
fragaria_vesca FvH4_7g19460 FvH4_7g19460 FvH4_7g19460 FvH4_7g19460
malus_domestica MD07G1174500.v1.1
prunus_persica Prupe.2G213300_v2.0.a1 Prupe.2G213300_v2.0.a1 Prupe.2G213300_v2.0.a1
pyrus_communis pycom07g16870
rosa_chinensis RchiOBHm_Chr1g0363331
rosa_laevigata RLG00000027573
rosa_roxburghii Rroxscaffold_4G00293270
rosa_rugosa Rorug01G0306400
rosa_samantha Rh1AG315200 Rh1CG294900
rosa_wichuraiana Rw1G027930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 293, 504, 786, 1916
AarI CACCTGC 2 cut(s) 1018, 1114
AatII GACGTC 1 cut(s) 2264
Acc36I ACCTGC 2 cut(s) 1018, 1114
AccB1I GGYRCC 1 cut(s) 1529
AccI GTMKAC 3 cut(s) 561, 756, 2258
AccII CGCG 1 cut(s) 198
AciI CCGC 2 cut(s) 198, 1247
AclI AACGTT 2 cut(s) 1641, 1793
AclWI GGATC 5 cut(s) 610, 1171, 1222, 1673, 1680
AcoI YGGCCR 1 cut(s) 436
AcyI GRCGYC 1 cut(s) 2261
AdeI CACNNNGTG 1 cut(s) 58
AfaI GTAC 3 cut(s) 746, 1600, 1605
AfiI CCNNNNNNNGG 3 cut(s) 985, 1192, 1495
AflII CTTAAG 2 cut(s) 2129, 2284
AgsI TTSAA 8 cut(s) 596, 611, 1172, 1274, 1676, 1898, 1935, 2223
AhdI GACNNNNNGTC 2 cut(s) 321, 2094
AjnI CCWGG 2 cut(s) 375, 1423
Alw21I GWGCWC 1 cut(s) 283
Alw26I GTCTC 4 cut(s) 73, 118, 1701, 2268
AlwI GGATC 5 cut(s) 610, 1171, 1222, 1673, 1680
AlwNI CAGNNNCTG 1 cut(s) 1994
AoxI GGCC 9 cut(s) 150, 268, 342, 436, 517, 675, 706, 927, 1570
ApeKI GCWGC 9 cut(s) 83, 162, 413, 416, 419, 653, 716, 851, 1004
Asp700I GAANNNNTTC 2 cut(s) 2077, 2214
AspLEI GCGC 3 cut(s) 217, 802, 1562
AspS9I GGNCC 4 cut(s) 150, 268, 383, 1818
AsuC2I CCSGG 2 cut(s) 256, 1716
AsuHPI GGTGA 7 cut(s) 16, 73, 948, 1095, 1290, 2036, 2073
AvaII GGWCC 2 cut(s) 383, 1818
BalI TGGCCA 1 cut(s) 438
BanI GGYRCC 1 cut(s) 1529
BanII GRGCYC 1 cut(s) 1948
BauI CACGAG 1 cut(s) 166
BbsI GAAGAC 4 cut(s) 32, 127, 320, 2285
Bbv12I GWGCWC 1 cut(s) 283
BbvI GCAGC 9 cut(s) 95, 174, 425, 428, 431, 640, 703, 838, 991
BccI CCATC 5 cut(s) 439, 482, 1140, 1961, 2036
BciT130I CCWGG 2 cut(s) 377, 1425
BcnI CCSGG 2 cut(s) 256, 1716
BcoDI GTCTC 4 cut(s) 73, 118, 1701, 2268
BfmI CTRYAG 1 cut(s) 852
BfrI CTTAAG 2 cut(s) 2129, 2284
BfuAI ACCTGC 2 cut(s) 1018, 1114
BisI GCNGC 9 cut(s) 84, 163, 414, 417, 420, 654, 717, 852, 1005
BlsI GCNGC 9 cut(s) 85, 164, 415, 418, 421, 655, 718, 853, 1006
Bme1390I CCNGG 4 cut(s) 256, 377, 1425, 1716
Bme18I GGWCC 2 cut(s) 383, 1818
BmeRI GACNNNNNGTC 2 cut(s) 321, 2094
BmgT120I GGNCC 4 cut(s) 150, 268, 383, 1818
BmiI GGNNCC 5 cut(s) 151, 472, 1198, 1245, 1531
BmrFI CCNGG 4 cut(s) 256, 377, 1425, 1716
BmsI GCATC 3 cut(s) 310, 665, 1981
BpiI GAAGAC 4 cut(s) 32, 127, 320, 2285
BplI GAGNNNNNCTC 2 cut(s) 1870, 1902
BpmI CTGGAG 1 cut(s) 489
Bpu10I CCTNAGC 2 cut(s) 1302, 2275
BpuEI CTTGAG 2 cut(s) 1872, 2153
BpuMI CCSGG 2 cut(s) 256, 1716
Bsa29I ATCGAT 1 cut(s) 1166
BsaBI GATNNNNATC 1 cut(s) 1419
BsaHI GRCGYC 1 cut(s) 2261
BsaI GGTCTC 1 cut(s) 118
BsaJI CCNNGG 7 cut(s) 255, 305, 345, 392, 980, 1821, 2084
BsaXI ACNNNNNCTCC 2 cut(s) 1067, 1097
Bsc4I CCNNNNNNNGG 3 cut(s) 985, 1192, 1495
Bse1I ACTGG 4 cut(s) 867, 959, 1036, 1346
Bse3DI GCAATG 2 cut(s) 827, 2041
Bse8I GATNNNNATC 1 cut(s) 1419
BseBI CCWGG 2 cut(s) 377, 1425
BseCI ATCGAT 1 cut(s) 1166
BseDI CCNNGG 7 cut(s) 255, 305, 345, 392, 980, 1821, 2084
BseGI GGATG 5 cut(s) 301, 656, 936, 1817, 1831
BseJI GATNNNNATC 1 cut(s) 1419
BseLI CCNNNNNNNGG 3 cut(s) 985, 1192, 1495
BseMI GCAATG 2 cut(s) 827, 2041
BseMII CTCAG 6 cut(s) 311, 701, 930, 1131, 1201, 1995
BseNI ACTGG 4 cut(s) 867, 959, 1036, 1346
BseRI GAGGAG 4 cut(s) 275, 278, 1175, 2021
BseXI GCAGC 9 cut(s) 95, 174, 425, 428, 431, 640, 703, 838, 991
BseYI CCCAGC 3 cut(s) 1288, 1444, 2066
BsgI GTGCAG 2 cut(s) 576, 990
Bsh1236I CGCG 1 cut(s) 198
BshFI GGCC 9 cut(s) 152, 270, 344, 438, 519, 677, 708, 929, 1572
BshNI GGYRCC 1 cut(s) 1529
BshVI ATCGAT 1 cut(s) 1166
BsiHKAI GWGCWC 1 cut(s) 283
BsiSI CCGG 3 cut(s) 153, 255, 1715
BslFI GGGAC 1 cut(s) 2162
BslI CCNNNNNNNGG 3 cut(s) 985, 1192, 1495
BsmAI GTCTC 4 cut(s) 73, 118, 1701, 2268
BsmBI CGTCTC 1 cut(s) 2268
BsmFI GGGAC 1 cut(s) 2162
BsmI GAATGC 4 cut(s) 783, 1253, 1982, 2279
BsnI GGCC 9 cut(s) 152, 270, 344, 438, 519, 677, 708, 929, 1572
Bso31I GGTCTC 1 cut(s) 118
Bsp1286I GDGCHC 2 cut(s) 283, 1948
Bsp143I GATC 7 cut(s) 602, 1163, 1214, 1295, 1678, 1685, 1771
Bsp19I CCATGG 3 cut(s) 305, 345, 980
BspACI CCGC 2 cut(s) 198, 1247
BspANI GGCC 9 cut(s) 152, 270, 344, 438, 519, 677, 708, 929, 1572
BspCNI CTCAG 6 cut(s) 310, 702, 929, 1132, 1200, 1996
BspDI ATCGAT 1 cut(s) 1166
BspFNI CGCG 1 cut(s) 198
BspLI GGNNCC 5 cut(s) 151, 472, 1198, 1245, 1531
BspMAI CTGCAG 1 cut(s) 856
BspMI ACCTGC 2 cut(s) 1018, 1114
BspPI GGATC 5 cut(s) 610, 1171, 1222, 1673, 1680
BspT107I GGYRCC 1 cut(s) 1529
BspTI CTTAAG 2 cut(s) 2129, 2284
BspTNI GGTCTC 1 cut(s) 118
BsrDI GCAATG 2 cut(s) 827, 2041
BsrI ACTGG 4 cut(s) 867, 959, 1036, 1346
BssECI CCNNGG 7 cut(s) 255, 305, 345, 392, 980, 1821, 2084
BssMI GATC 7 cut(s) 602, 1163, 1214, 1295, 1678, 1685, 1771
BssNAI GTATAC 1 cut(s) 562
BssNI GRCGYC 1 cut(s) 2261
BssSI CACGAG 1 cut(s) 166
BssT1I CCWWGG 4 cut(s) 305, 345, 392, 980
Bst1107I GTATAC 1 cut(s) 562
Bst2BI CACGAG 1 cut(s) 166
Bst2UI CCWGG 2 cut(s) 377, 1425
Bst4CI ACNGT 9 cut(s) 133, 554, 1480, 1603, 1801, 1832, 1838, 1941, 2247
Bst6I CTCTTC 6 cut(s) 121, 393, 612, 687, 2111, 2138
BstACI GRCGYC 1 cut(s) 2261
BstAFI CTTAAG 2 cut(s) 2129, 2284
BstC8I GCNNGC 9 cut(s) 196, 231, 517, 633, 1251, 1363, 1384, 1581, 2157
BstDSI CCRYGG 3 cut(s) 305, 345, 980
BstF5I GGATG 5 cut(s) 301, 656, 936, 1817, 1831
BstFNI CGCG 1 cut(s) 198
BstHHI GCGC 3 cut(s) 217, 802, 1562
BstKTI GATC 7 cut(s) 605, 1166, 1217, 1298, 1681, 1688, 1774
BstMAI GTCTC 4 cut(s) 73, 118, 1701, 2268
BstMBI GATC 7 cut(s) 602, 1163, 1214, 1295, 1678, 1685, 1771
BstMWI GCNNNNNNNGC 3 cut(s) 214, 419, 1010
BstNI CCWGG 2 cut(s) 377, 1425
BstNSI RCATGY 3 cut(s) 233, 1652, 1874
BstSCI CCNGG 4 cut(s) 254, 375, 1423, 1714
BstSFI CTRYAG 1 cut(s) 852
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 9 cut(s) 95, 174, 425, 428, 431, 640, 703, 838, 991
BstV2I GAAGAC 4 cut(s) 32, 127, 320, 2285
BstX2I RGATCY 2 cut(s) 602, 1678
BstYI RGATCY 2 cut(s) 602, 1678
BstZ17I GTATAC 1 cut(s) 562
Bsu15I ATCGAT 1 cut(s) 1166
BsuRI GGCC 9 cut(s) 152, 270, 344, 438, 519, 677, 708, 929, 1572
BsuTUI ATCGAT 1 cut(s) 1166
BtgI CCRYGG 3 cut(s) 305, 345, 980
BtsCI GGATG 5 cut(s) 301, 656, 936, 1817, 1831
BtsI GCAGTG 1 cut(s) 215
BtsIMutI CAGTG 8 cut(s) 129, 215, 798, 860, 966, 1837, 1843, 2243
BveI ACCTGC 2 cut(s) 1018, 1114
Cac8I GCNNGC 9 cut(s) 196, 231, 517, 633, 1251, 1363, 1384, 1581, 2157
CaiI CAGNNNCTG 1 cut(s) 1994
CfoI GCGC 3 cut(s) 217, 802, 1562
Cfr13I GGNCC 4 cut(s) 150, 268, 383, 1818
ClaI ATCGAT 1 cut(s) 1166
Csp6I GTAC 3 cut(s) 745, 1599, 1604
CviQI GTAC 3 cut(s) 745, 1599, 1604
DpnI GATC 7 cut(s) 604, 1165, 1216, 1297, 1680, 1687, 1773
DpnII GATC 7 cut(s) 602, 1163, 1214, 1295, 1678, 1685, 1771
DraIII CACNNNGTG 1 cut(s) 58
DriI GACNNNNNGTC 2 cut(s) 321, 2094
EaeI YGGCCR 1 cut(s) 436
Eam1104I CTCTTC 6 cut(s) 121, 393, 612, 687, 2111, 2138
Eam1105I GACNNNNNGTC 2 cut(s) 321, 2094
EarI CTCTTC 6 cut(s) 121, 393, 612, 687, 2111, 2138
EciI GGCGGA 1 cut(s) 1236
Eco130I CCWWGG 4 cut(s) 305, 345, 392, 980
Eco147I AGGCCT 1 cut(s) 708
Eco24I GRGCYC 1 cut(s) 1948
Eco31I GGTCTC 1 cut(s) 118
Eco47I GGWCC 2 cut(s) 383, 1818
EcoO109I RGGNCCY 1 cut(s) 268
EcoRII CCWGG 2 cut(s) 375, 1423
EcoT14I CCWWGG 4 cut(s) 305, 345, 392, 980
EcoT22I ATGCAT 2 cut(s) 1872, 1984
EcoT38I GRGCYC 1 cut(s) 1948
ErhI CCWWGG 4 cut(s) 305, 345, 392, 980
Esp3I CGTCTC 1 cut(s) 2268
FalI AAGNNNNNCTT 2 cut(s) 2148, 2180
FaqI GGGAC 1 cut(s) 2162
FauNDI CATATG 1 cut(s) 1261
FblI GTMKAC 3 cut(s) 561, 756, 2258
Fnu4HI GCNGC 9 cut(s) 84, 163, 414, 417, 420, 654, 717, 852, 1005
FokI GGATG 5 cut(s) 288, 643, 923, 1824, 1838
FriOI GRGCYC 1 cut(s) 1948
Fsp4HI GCNGC 9 cut(s) 84, 163, 414, 417, 420, 654, 717, 852, 1005
GlaI GCGC 3 cut(s) 216, 801, 1561
GluI GCNGC 9 cut(s) 84, 163, 414, 417, 420, 654, 717, 852, 1005
GsaI CCCAGC 3 cut(s) 1292, 1448, 2070
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 9 cut(s) 152, 270, 344, 438, 519, 677, 708, 929, 1572
HapII CCGG 3 cut(s) 153, 255, 1715
HhaI GCGC 3 cut(s) 217, 802, 1562
Hin1I GRCGYC 1 cut(s) 2261
Hin6I GCGC 3 cut(s) 215, 800, 1560
HinP1I GCGC 3 cut(s) 215, 800, 1560
HincII GTYRAC 1 cut(s) 2259
HindII GTYRAC 1 cut(s) 2259
HindIII AAGCTT 2 cut(s) 910, 2225
HinfI GANTC 6 cut(s) 49, 66, 89, 1168, 1420, 2101
HpaII CCGG 3 cut(s) 153, 255, 1715
HphI GGTGA 7 cut(s) 16, 73, 948, 1095, 1290, 2036, 2073
Hpy166II GTNNAC 8 cut(s) 97, 562, 757, 897, 1639, 1797, 2243, 2259
Hpy188I TCNGA 7 cut(s) 43, 356, 1115, 1311, 1760, 2005, 2100
Hpy188III TCNNGA 7 cut(s) 468, 1271, 1436, 1629, 1769, 1876, 2110
Hpy8I GTNNAC 8 cut(s) 97, 562, 757, 897, 1639, 1797, 2243, 2259
Hpy99I CGWCG 1 cut(s) 2263
HpyAV CCTTC 3 cut(s) 940, 1745, 2174
HpyCH4III ACNGT 9 cut(s) 133, 554, 1480, 1603, 1801, 1832, 1838, 1941, 2247
HpyCH4IV ACGT 5 cut(s) 1641, 1793, 2052, 2090, 2261
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 419, 1010
HpySE526I ACGT 5 cut(s) 1641, 1793, 2052, 2090, 2261
Hsp92I GRCGYC 1 cut(s) 2261
HspAI GCGC 3 cut(s) 215, 800, 1560
Kzo9I GATC 7 cut(s) 602, 1163, 1214, 1295, 1678, 1685, 1771
LmnI GCTCC 3 cut(s) 470, 1022, 2164
Lsp1109I GCAGC 9 cut(s) 95, 174, 425, 428, 431, 640, 703, 838, 991
LweI GCATC 3 cut(s) 310, 665, 1981
MaeII ACGT 5 cut(s) 1641, 1793, 2052, 2090, 2261
MaeIII GTNAC 7 cut(s) 50, 954, 1101, 1789, 1832, 2024, 2194
MalI GATC 7 cut(s) 604, 1165, 1216, 1297, 1680, 1687, 1773
MboI GATC 7 cut(s) 602, 1163, 1214, 1295, 1678, 1685, 1771
MfeI CAATTG 1 cut(s) 596
MflI RGATCY 2 cut(s) 602, 1678
MhlI GDGCHC 2 cut(s) 283, 1948
MlsI TGGCCA 1 cut(s) 438
MluNI TGGCCA 1 cut(s) 438
MlyI GAGTC 3 cut(s) 58, 75, 2095
MmeI TCCRAC 2 cut(s) 165, 1796
Mox20I TGGCCA 1 cut(s) 438
Mph1103I ATGCAT 2 cut(s) 1872, 1984
MroXI GAANNNNTTC 2 cut(s) 2077, 2214
MscI TGGCCA 1 cut(s) 438
MslI CAYNNNNRTG 1 cut(s) 59
Msp20I TGGCCA 1 cut(s) 438
MspA1I CMGCKG 3 cut(s) 86, 851, 1099
MspCI CTTAAG 2 cut(s) 2129, 2284
MspI CCGG 3 cut(s) 153, 255, 1715
MspR9I CCNGG 4 cut(s) 256, 377, 1425, 1716
MunI CAATTG 1 cut(s) 596
Mva1269I GAATGC 4 cut(s) 783, 1253, 1982, 2279
MvaI CCWGG 2 cut(s) 377, 1425
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 3 cut(s) 214, 419, 1010
NciI CCSGG 2 cut(s) 256, 1716
NcoI CCATGG 3 cut(s) 305, 345, 980
NdeI CATATG 1 cut(s) 1261
NdeII GATC 7 cut(s) 602, 1163, 1214, 1295, 1678, 1685, 1771
NlaIV GGNNCC 5 cut(s) 151, 472, 1198, 1245, 1531
NmuCI GTSAC 5 cut(s) 50, 954, 1101, 1832, 2024
NsiI ATGCAT 2 cut(s) 1872, 1984
NspI RCATGY 3 cut(s) 233, 1652, 1874
PaeI GCATGC 1 cut(s) 233
PaqCI CACCTGC 2 cut(s) 1018, 1114
PceI AGGCCT 1 cut(s) 708
PctI GAATGC 4 cut(s) 783, 1253, 1982, 2279
PdmI GAANNNNTTC 2 cut(s) 2077, 2214
PfeI GAWTC 3 cut(s) 89, 1168, 1420
PflFI GACNNNGTC 1 cut(s) 131
PfoI TCCNGGA 1 cut(s) 1714
PkrI GCNGC 9 cut(s) 85, 164, 415, 418, 421, 655, 718, 853, 1006
PleI GAGTC 3 cut(s) 57, 74, 2095
PpsI GAGTC 3 cut(s) 57, 74, 2095
PsiI TTATAA 4 cut(s) 293, 504, 786, 1916
Psp1406I AACGTT 2 cut(s) 1641, 1793
Psp6I CCWGG 2 cut(s) 375, 1423
PspFI CCCAGC 3 cut(s) 1288, 1444, 2066
PspGI CCWGG 2 cut(s) 375, 1423
PspN4I GGNNCC 5 cut(s) 151, 472, 1198, 1245, 1531
PspPI GGNCC 4 cut(s) 150, 268, 383, 1818
PstI CTGCAG 1 cut(s) 856
PstNI CAGNNNCTG 1 cut(s) 1994
PsuI RGATCY 2 cut(s) 602, 1678
PsyI GACNNNGTC 1 cut(s) 131
PvuII CAGCTG 3 cut(s) 86, 851, 1099
RsaI GTAC 3 cut(s) 746, 1600, 1605
RsaNI GTAC 3 cut(s) 745, 1599, 1604
RseI CAYNNNNRTG 1 cut(s) 59
SalI GTCGAC 1 cut(s) 2257
SatI GCNGC 9 cut(s) 84, 163, 414, 417, 420, 654, 717, 852, 1005
Sau3AI GATC 7 cut(s) 602, 1163, 1214, 1295, 1678, 1685, 1771
Sau96I GGNCC 4 cut(s) 150, 268, 383, 1818
SchI GAGTC 3 cut(s) 58, 75, 2095
ScrFI CCNGG 4 cut(s) 256, 377, 1425, 1716
SduI GDGCHC 2 cut(s) 283, 1948
SfaNI GCATC 3 cut(s) 310, 665, 1981
SfcI CTRYAG 1 cut(s) 852
SinI GGWCC 2 cut(s) 383, 1818
SmiMI CAYNNNNRTG 1 cut(s) 59
SmlI CTYRAG 4 cut(s) 1887, 2129, 2168, 2284
SmoI CTYRAG 4 cut(s) 1887, 2129, 2168, 2284
SphI GCATGC 1 cut(s) 233
SseBI AGGCCT 1 cut(s) 708
SsiI CCGC 2 cut(s) 198, 1247
SspI AATATT 1 cut(s) 1040
StuI AGGCCT 1 cut(s) 708
StyD4I CCNGG 4 cut(s) 254, 375, 1423, 1714
StyI CCWWGG 4 cut(s) 305, 345, 392, 980
TaaI ACNGT 9 cut(s) 133, 554, 1480, 1603, 1801, 1832, 1838, 1941, 2247
TaiI ACGT 5 cut(s) 1644, 1796, 2055, 2093, 2264
TaqI TCGA 6 cut(s) 1166, 1684, 1768, 1920, 2208, 2258
TaqII GACCGA 1 cut(s) 1835
TfiI GAWTC 3 cut(s) 89, 1168, 1420
TscAI CASTG 8 cut(s) 136, 215, 798, 867, 966, 1837, 1843, 2250
TseFI GTSAC 5 cut(s) 50, 954, 1101, 1832, 2024
TseI GCWGC 9 cut(s) 83, 162, 413, 416, 419, 653, 716, 851, 1004
Tsp45I GTSAC 5 cut(s) 50, 954, 1101, 1832, 2024
TspDTI ATGAA 6 cut(s) 922, 1049, 1360, 1500, 1508, 1980
TspRI CASTG 8 cut(s) 136, 215, 798, 867, 966, 1837, 1843, 2250
Tth111I GACNNNGTC 1 cut(s) 131
Vha464I CTTAAG 2 cut(s) 2129, 2284
VpaK11BI GGWCC 2 cut(s) 383, 1818
XceI RCATGY 3 cut(s) 233, 1652, 1874
XmiI GTMKAC 3 cut(s) 561, 756, 2258
XmnI GAANNNNTTC 2 cut(s) 2077, 2214
ZraI GACGTC 1 cut(s) 2262
Zsp2I ATGCAT 2 cut(s) 1872, 1984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.