Rh1CG294900

Endoplasmic reticulum metallopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0011223
Physical Location & Seq
Reverse (-)
56784121 .. 56790107
5987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG294900.1

Sequence Viewer

Length: 2733 bp
ATGCGACGGAAGCCCCAGACTGGTTCCTCTTCAGCTCCCGAACCACAACCAGAACAAGAACCAACTCAAAACCCAAGTGTCACCACCCCATGGGTTGTTCAGAGGCCACAGCGATCACCCTTTGTCTGGCTGACCTTGTTGGCAGCCATCACATACTCGTGTTGGGGCGTTTATCATTACCAGTTCGAGTCCCTCCCCGCCCCTCTCACTCCTGACCAGGCCGGAAAGCGAGGCTTCTCCGAGTTCTCTGCTCGGAAACACGTCAAGGTTCTGACCCAGTTGGGCCCTCACCCTGTTGCCTCTGAAGCCATCACTCTTGCTCAGCAGTATGTATTGGCAGAAGCAGAGGAGATTAAGAAAACGGCTCACTGGGAAGTTGATGTGGAGGTGGACTCGTTTGTTGCGAAAACCGGAGCTAATCAAATGAAGAGTGGATTATTTAAGGGCAAAACACTTGTGTATTCGGATTTGAATCACATTGTTGTAAGAATCTCGCCCAAGTATTCACCTATTTCTGTGGACAATGCTGTTCTCGTGTCTTCTCACATTGACACTGTCTTCTCAACAGGAGGGGCCGGAGATTGCAGTTCGTGTGTGGCTGTTATGTTGGAACTTGCTCGTGGAGTTTCGCAGTGGGCGCATGGATTTAAGCATGCTGTTATATTTTTGTTTAACACTGGGGAGGAGGAAGGTCTAGATGGTGCTCATAGCTTTATAACTCAGCATCCATGGAGCAGTACGATTCGTCTGGCTATTGATTTGGAGGCCATGGGTATTGGAGGGAAGTCTGGCATATTTCAGGCCGGCCCTCATCCTTGGGCTATTGAGAATTATGCTGCAGTAGCGAAATACCCATCTGGCCACATAATAGGACAGAATATTTTTTCCTCGGGAGCCATCAAATCTGCAACAGATTTCCAAGTATACAAAGAGTTAGCTGGCCTTTCAGGCCTTGACTTTGCTTACTCAGATAACAGTGCAGTATACCACACGAAGAATGACAAATTCGAGCTTCTTCAATTGGGATCTCTTCAACATCTTGGAGAAAATATGCTTGCTTTTCTGCTCCGCGTCGCTGCATCCTCTCATCTTCCAAAAGCCAGTACAATGGAAGAAGAGGATAAAACAGGCCTGAGTTCTGCCATATATTTTGACATTTTGGGGATGTACATGATTGTCTACCGTCAACGCTTCGCCAGAATGCTTCATAATTCAGTGATAGCGCAATCACTTCTTATATGGACTACATCATTGCTTATGGGTGGATATCCAGCTGCAGTATCATTGGTCTTGTCATGTTTAAGTGTCATTCTTATGTGGACATTTGCATTAAGTTTCTCTGTTATTGTGGCCTTTATTATACCCCTAATATCATCATCACCAGTGCCATATGTCGCAAACCCATGGCTTGTAGTTGGGTTATTCGCTGCACCTGCTCTGCTTGGAGCATTAACTGGTCAATATTTGGGTTATCTTGTTCTTCATACGTATTTGGCAAACGCTTACTCCAAGAAAAAACAGCTGTCACATGCTATCCGAGCCGATTTGGTTAAATTAGAGGCTGAAAGATGGCTCTACAAAGCTGGATCAATTCAATGGCTCATTCTCCTCTGTTTGGGAACCTATTACAGAATTGGATCGTCTTATCTGGCTCTTGCTTGGTTGGTTCCACCAGCATTCGCATATGGCTTTCTTGAAGCAACTCTAAGCCCAGTCCGATTACCTAAGCCACTGAAACTTGCAACTCTGCTGATAGGATTAGCTGTACCGATAGTACTTTCTGCTGGAGTATTCATTAGGGTGGCTGGCACAATAATCGGGACCATGGTTCGTTTTGATAGGAATCCTGGTGGCACTCCTGATTGGCTGGGAAATGTTATACTTGCTGTTTTTGTTTCTGCTGTCATGTGCCTGACTTTGGTGTATCTTCTTTCATACATTCATCTTTCAGGTGCCAAGAGATTGATTGTTCTCTCAACTTGCGCCTTGTTTTGCCTCTCGCTTGCTCTTGTATTATCTGGTACTGTACCAGCATTTACCAAAGACACATCAAGAGCTGTAAATGTTGTGCATGTTGTAGATACTACAAGAAGCATTGAAGATCCTAGATCCTATGTGTCACTGTTTTCTTCAACTCCTGGAAAATTGACAAAGGAGGTTGAGCTAATTAATGAAGGTTTCAGATGTGGTCGAGATCAGGTCTTTGATTTTGTAACTTTTACAGTGAAATACGGCTGTTGGACTGAGGATGACAGTGACAATGGGTGGAGTGAAGCAGACATTCCCATAATGCATGTTCAGAGTGATACTCAAGGTATTGAAAGAACAACACAAGTTTTAATTGATACAAAGGGTTCATTACGGTGGTCTCTTGCAATAAACATGGATGAAATACGGGATTTTGCATTCACAGATGCTGGAAACTCTGAGGAATTGGTTTCAGTTGGTGACAAGAGCGCTGTAGATGGTTGGCATGTTATTCAGTTTGCCGGGGGAAACAATTCACCAACGAAGTTTGGTCTGACTCTCTTCTGGACCAAAAACTCTACCCTTAAGGCGGAGGGGAAGAGAGACGAGCAAGCTCCCCTTCTCAAGTTAAGAACTGATATGGACATTGTAACACCGAAAGTCGAAAGAATTCTTTCAAAGCTTCCTACTTGGTGTTCACTGTTTGGCAAGTCCACGTCTCCCTACACCTTTGCATTCTTAAGTAGTCTTCCTGTTGATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M28

Protein Analysis

910

Amino Acids

99.68

Weight (kDa)

6.75

Isoelectric Point (pI)

35.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M28 PF04389 160 - 354 1.4e-44 Peptidase family M28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20660 AT5G20660 AT5G20660 AT5G20660
fragaria_vesca FvH4_7g19460 FvH4_7g19460 FvH4_7g19460 FvH4_7g19460
malus_domestica MD07G1174500.v1.1
prunus_persica Prupe.2G213300_v2.0.a1 Prupe.2G213300_v2.0.a1 Prupe.2G213300_v2.0.a1
pyrus_communis pycom07g16870
rosa_chinensis RchiOBHm_Chr1g0363331
rosa_laevigata RLG00000027573
rosa_roxburghii Rroxscaffold_4G00293270
rosa_rugosa Rorug01G0306400
rosa_samantha Rh1AG315200 Rh1CG294900
rosa_wichuraiana Rw1G027930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 716
AarI CACCTGC 1 cut(s) 1441
Acc36I ACCTGC 1 cut(s) 1441
AccB1I GGYRCC 1 cut(s) 1952
AccB7I CCANNNNNTGG 1 cut(s) 90
AccI GTMKAC 3 cut(s) 924, 984, 1179
AccII CGCG 1 cut(s) 1071
AciI CCGC 3 cut(s) 198, 1069, 2558
AclWI GGATC 5 cut(s) 1033, 1594, 1645, 2096, 2103
AcoI YGGCCR 1 cut(s) 859
AcsI RAATTY 2 cut(s) 1004, 2637
AcuI CTGAAG 2 cut(s) 15, 324
AfaI GTAC 7 cut(s) 739, 1105, 1169, 1767, 1776, 2023, 2028
AfeI AGCGCT 1 cut(s) 2458
AfiI CCNNNNNNNGG 6 cut(s) 20, 90, 126, 1615, 1918, 2557
AflII CTTAAG 2 cut(s) 2552, 2707
AflIII ACRYGT 1 cut(s) 259
AgsI TTSAA 9 cut(s) 472, 1019, 1034, 1595, 1697, 2099, 2133, 2321, 2646
AjiI CACGTC 2 cut(s) 262, 2685
AjnI CCWGG 3 cut(s) 216, 1846, 2137
Alw21I GWGCWC 1 cut(s) 706
Alw26I GTCTC 3 cut(s) 2373, 2565, 2691
AlwI GGATC 5 cut(s) 1033, 1594, 1645, 2096, 2103
AlwNI CAGNNNCTG 1 cut(s) 2417
Ama87I CYCGRG 1 cut(s) 889
Aor51HI AGCGCT 1 cut(s) 2458
ApaI GGGCCC 1 cut(s) 287
ApeKI GCWGC 5 cut(s) 143, 836, 1076, 1274, 1427
ApoI RAATTY 2 cut(s) 1004, 2637
AseI ATTAAT 1 cut(s) 2169
Asp700I GAANNNNTTC 3 cut(s) 2500, 2637, 2641
AspLEI GCGC 4 cut(s) 640, 1225, 1985, 2459
AspS9I GGNCC 6 cut(s) 283, 284, 573, 806, 1821, 2535
AsuC2I CCSGG 1 cut(s) 2491
AsuHPI GGTGA 7 cut(s) 73, 108, 281, 498, 1371, 2459, 2496
AvaI CYCGRG 1 cut(s) 889
AvaII GGWCC 2 cut(s) 1821, 2535
BaeGI GKGCMC 1 cut(s) 287
BalI TGGCCA 1 cut(s) 861
BanI GGYRCC 1 cut(s) 1952
BanII GRGCYC 1 cut(s) 287
BauI CACGAG 3 cut(s) 157, 533, 618
BbsI GAAGAC 3 cut(s) 531, 550, 2708
Bbv12I GWGCWC 1 cut(s) 706
BbvI GCAGC 5 cut(s) 155, 823, 1063, 1261, 1414
BccI CCATC 7 cut(s) 155, 317, 692, 862, 905, 1563, 2459
BceAI ACGGC 2 cut(s) 378, 2248
BcgI CGANNNNNNTGC 4 cut(s) 230, 264, 1798, 1832
BciT130I CCWGG 3 cut(s) 218, 1848, 2139
BcnI CCSGG 1 cut(s) 2491
BcoDI GTCTC 3 cut(s) 2373, 2565, 2691
BfaI CTAG 2 cut(s) 695, 2106
BfmI CTRYAG 3 cut(s) 837, 1275, 2460
BfoI RGCGCY 1 cut(s) 2460
BfrI CTTAAG 2 cut(s) 2552, 2707
BfuAI ACCTGC 1 cut(s) 1441
BglI GCCNNNNNGGC 1 cut(s) 948
BisI GCNGC 5 cut(s) 144, 837, 1077, 1275, 1428
BlpI GCTNAGC 1 cut(s) 321
BlsI GCNGC 5 cut(s) 145, 838, 1078, 1276, 1429
BmcAI AGTACT 1 cut(s) 1776
Bme1390I CCNGG 4 cut(s) 218, 1848, 2139, 2491
Bme18I GGWCC 2 cut(s) 1821, 2535
BmeT110I CYCGRG 1 cut(s) 889
BmgBI CACGTC 2 cut(s) 262, 2685
BmgT120I GGNCC 6 cut(s) 283, 284, 573, 806, 1821, 2535
BmiI GGNNCC 8 cut(s) 25, 285, 574, 895, 1621, 1668, 1822, 1954
BmrFI CCNGG 4 cut(s) 218, 1848, 2139, 2491
BmrI ACTGGG 4 cut(s) 271, 379, 687, 1706
BmsI GCATC 3 cut(s) 733, 1088, 2404
BmuI ACTGGG 4 cut(s) 271, 379, 687, 1706
BpiI GAAGAC 3 cut(s) 531, 550, 2708
BplI GAGNNNNNCTC 4 cut(s) 377, 409, 2293, 2325
BpmI CTGGAG 1 cut(s) 1806
Bpu10I CCTNAGC 1 cut(s) 1725
Bpu1102I GCTNAGC 1 cut(s) 321
BpuEI CTTGAG 2 cut(s) 2295, 2576
BpuMI CCSGG 1 cut(s) 2491
BsaAI YACGTR 1 cut(s) 1488
BsaBI GATNNNNATC 2 cut(s) 471, 1842
BsaI GGTCTC 1 cut(s) 2373
BsaJI CCNNGG 8 cut(s) 89, 728, 768, 815, 888, 1403, 1824, 2490
BsaWI WCCGGW 1 cut(s) 410
BsaXI ACNNNNNCTCC 2 cut(s) 1490, 1520
Bsc4I CCNNNNNNNGG 6 cut(s) 20, 90, 126, 1615, 1918, 2557
Bse118I RCCGGY 1 cut(s) 803
Bse1I ACTGG 9 cut(s) 25, 181, 277, 374, 682, 1101, 1382, 1459, 1712
Bse3DI GCAATG 1 cut(s) 1250
Bse8I GATNNNNATC 2 cut(s) 471, 1842
BseBI CCWGG 3 cut(s) 218, 1848, 2139
BseDI CCNNGG 8 cut(s) 89, 728, 768, 815, 888, 1403, 1824, 2490
BseGI GGATG 6 cut(s) 724, 811, 1079, 1170, 2254, 2392
BseJI GATNNNNATC 2 cut(s) 471, 1842
BseLI CCNNNNNNNGG 6 cut(s) 20, 90, 126, 1615, 1918, 2557
BseMI GCAATG 1 cut(s) 1250
BseMII CTCAG 6 cut(s) 335, 734, 981, 1124, 2235, 2418
BseNI ACTGG 9 cut(s) 25, 181, 277, 374, 682, 1101, 1382, 1459, 1712
BseRI GAGGAG 3 cut(s) 362, 698, 1598
BseSI GKGCMC 1 cut(s) 287
BseXI GCAGC 5 cut(s) 155, 823, 1063, 1261, 1414
BseYI CCCAGC 1 cut(s) 1867
BsgI GTGCAG 2 cut(s) 999, 1413
Bsh1236I CGCG 1 cut(s) 1071
BshNI GGYRCC 1 cut(s) 1952
BsiHKAI GWGCWC 1 cut(s) 706
BsiHKCI CYCGRG 1 cut(s) 889
BsiSI CCGG 5 cut(s) 222, 411, 576, 804, 2490
BslFI GGGAC 2 cut(s) 175, 1834
BslI CCNNNNNNNGG 6 cut(s) 20, 90, 126, 1615, 1918, 2557
BsmAI GTCTC 3 cut(s) 2373, 2565, 2691
BsmBI CGTCTC 2 cut(s) 2565, 2691
BsmFI GGGAC 2 cut(s) 175, 1834
BsmI GAATGC 4 cut(s) 1206, 1676, 2405, 2702
Bso31I GGTCTC 1 cut(s) 2373
BsoBI CYCGRG 1 cut(s) 889
Bsp120I GGGCCC 1 cut(s) 283
Bsp1286I GDGCHC 2 cut(s) 287, 706
Bsp1407I TGTACA 1 cut(s) 1167
Bsp143I GATC 7 cut(s) 113, 1025, 1586, 1637, 2101, 2108, 2194
Bsp1720I GCTNAGC 1 cut(s) 321
Bsp19I CCATGG 5 cut(s) 89, 728, 768, 1403, 1824
BspACI CCGC 3 cut(s) 198, 1069, 2558
BspCNI CTCAG 6 cut(s) 334, 733, 980, 1125, 2236, 2419
BspFNI CGCG 1 cut(s) 1071
BspLI GGNNCC 8 cut(s) 25, 285, 574, 895, 1621, 1668, 1822, 1954
BspMAI CTGCAG 2 cut(s) 841, 1279
BspMI ACCTGC 1 cut(s) 1441
BspPI GGATC 5 cut(s) 1033, 1594, 1645, 2096, 2103
BspT107I GGYRCC 1 cut(s) 1952
BspTI CTTAAG 2 cut(s) 2552, 2707
BspTNI GGTCTC 1 cut(s) 2373
BsrDI GCAATG 1 cut(s) 1250
BsrFI RCCGGY 1 cut(s) 803
BsrGI TGTACA 1 cut(s) 1167
BsrI ACTGG 9 cut(s) 25, 181, 277, 374, 682, 1101, 1382, 1459, 1712
BssAI RCCGGY 1 cut(s) 803
BssECI CCNNGG 8 cut(s) 89, 728, 768, 815, 888, 1403, 1824, 2490
BssMI GATC 7 cut(s) 113, 1025, 1586, 1637, 2101, 2108, 2194
BssNAI GTATAC 2 cut(s) 925, 985
BssSI CACGAG 3 cut(s) 157, 533, 618
BssT1I CCWWGG 6 cut(s) 89, 728, 768, 815, 1403, 1824
Bst1107I GTATAC 2 cut(s) 925, 985
Bst2BI CACGAG 3 cut(s) 157, 533, 618
Bst2UI CCWGG 3 cut(s) 218, 1848, 2139
Bst4CI ACNGT 9 cut(s) 556, 977, 1184, 2026, 2124, 2224, 2255, 2364, 2670
Bst6I CTCTTC 6 cut(s) 34, 422, 1035, 1110, 2534, 2561
BstAFI CTTAAG 2 cut(s) 2552, 2707
BstAUI TGTACA 1 cut(s) 1167
BstBAI YACGTR 1 cut(s) 1488
BstC8I GCNNGC 7 cut(s) 654, 805, 940, 1056, 1807, 2004, 2580
BstDEI CTNAG 8 cut(s) 321, 720, 967, 1133, 1706, 1725, 2244, 2427
BstDSI CCRYGG 5 cut(s) 89, 728, 768, 1403, 1824
BstF5I GGATG 6 cut(s) 724, 811, 1079, 1170, 2254, 2392
BstFNI CGCG 1 cut(s) 1071
BstH2I RGCGCY 1 cut(s) 2460
BstHHI GCGC 4 cut(s) 640, 1225, 1985, 2459
BstKTI GATC 7 cut(s) 116, 1028, 1589, 1640, 2104, 2111, 2197
BstMAI GTCTC 3 cut(s) 2373, 2565, 2691
BstMBI GATC 7 cut(s) 113, 1025, 1586, 1637, 2101, 2108, 2194
BstMWI GCNNNNNNNGC 7 cut(s) 10, 305, 637, 842, 948, 1433, 1538
BstNI CCWGG 3 cut(s) 218, 1848, 2139
BstNSI RCATGY 5 cut(s) 656, 1532, 2075, 2297, 2477
BstSCI CCNGG 4 cut(s) 216, 1846, 2137, 2489
BstSFI CTRYAG 3 cut(s) 837, 1275, 2460
BstSLI GKGCMC 1 cut(s) 287
BstSNI TACGTA 1 cut(s) 1488
BstUI CGCG 1 cut(s) 1071
BstV1I GCAGC 5 cut(s) 155, 823, 1063, 1261, 1414
BstV2I GAAGAC 3 cut(s) 531, 550, 2708
BstX2I RGATCY 3 cut(s) 1025, 2101, 2108
BstXI CCANNNNNNTGG 1 cut(s) 1108
BstYI RGATCY 3 cut(s) 1025, 2101, 2108
BstZ17I GTATAC 2 cut(s) 925, 985
BtgI CCRYGG 5 cut(s) 89, 728, 768, 1403, 1824
BtrI CACGTC 2 cut(s) 262, 2685
BtsCI GGATG 6 cut(s) 724, 811, 1079, 1170, 2254, 2392
BtsI GCAGTG 1 cut(s) 638
BveI ACCTGC 1 cut(s) 1441
Cac8I GCNNGC 7 cut(s) 654, 805, 940, 1056, 1807, 2004, 2580
CaiI CAGNNNCTG 1 cut(s) 2417
CfoI GCGC 4 cut(s) 640, 1225, 1985, 2459
Cfr10I RCCGGY 1 cut(s) 803
Cfr13I GGNCC 6 cut(s) 283, 284, 573, 806, 1821, 2535
CseI GACGC 1 cut(s) 1060
Csp6I GTAC 7 cut(s) 738, 1104, 1168, 1766, 1775, 2022, 2027
CviQI GTAC 7 cut(s) 738, 1104, 1168, 1766, 1775, 2022, 2027
DdeI CTNAG 8 cut(s) 321, 720, 967, 1133, 1706, 1725, 2244, 2427
DpnI GATC 7 cut(s) 115, 1027, 1588, 1639, 2103, 2110, 2196
DpnII GATC 7 cut(s) 113, 1025, 1586, 1637, 2101, 2108, 2194
EaeI YGGCCR 1 cut(s) 859
Eam1104I CTCTTC 6 cut(s) 34, 422, 1035, 1110, 2534, 2561
EarI CTCTTC 6 cut(s) 34, 422, 1035, 1110, 2534, 2561
EciI GGCGGA 1 cut(s) 2573
Eco105I TACGTA 1 cut(s) 1488
Eco130I CCWWGG 6 cut(s) 89, 728, 768, 815, 1403, 1824
Eco147I AGGCCT 2 cut(s) 951, 1131
Eco24I GRGCYC 1 cut(s) 287
Eco31I GGTCTC 1 cut(s) 2373
Eco32I GATATC 1 cut(s) 1268
Eco47I GGWCC 2 cut(s) 1821, 2535
Eco47III AGCGCT 1 cut(s) 2458
Eco57I CTGAAG 2 cut(s) 15, 324
Eco88I CYCGRG 1 cut(s) 889
EcoO109I RGGNCCY 1 cut(s) 284
EcoRI GAATTC 1 cut(s) 2637
EcoRII CCWGG 3 cut(s) 216, 1846, 2137
EcoRV GATATC 1 cut(s) 1268
EcoT14I CCWWGG 6 cut(s) 89, 728, 768, 815, 1403, 1824
EcoT22I ATGCAT 1 cut(s) 2295
EcoT38I GRGCYC 1 cut(s) 287
ErhI CCWWGG 6 cut(s) 89, 728, 768, 815, 1403, 1824
Esp3I CGTCTC 2 cut(s) 2565, 2691
FalI AAGNNNNNCTT 4 cut(s) 218, 250, 2571, 2603
FaqI GGGAC 2 cut(s) 175, 1834
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 2 cut(s) 1390, 1684
FblI GTMKAC 3 cut(s) 924, 984, 1179
Fnu4HI GCNGC 5 cut(s) 144, 837, 1077, 1275, 1428
FokI GGATG 6 cut(s) 711, 798, 1066, 1177, 2261, 2399
FriOI GRGCYC 1 cut(s) 287
FseI GGCCGGCC 1 cut(s) 807
Fsp4HI GCNGC 5 cut(s) 144, 837, 1077, 1275, 1428
FspBI CTAG 2 cut(s) 695, 2106
GlaI GCGC 4 cut(s) 639, 1224, 1984, 2458
GluI GCNGC 5 cut(s) 144, 837, 1077, 1275, 1428
GsaI CCCAGC 1 cut(s) 1871
GsuI CTGGAG 1 cut(s) 1806
HaeII RGCGCY 1 cut(s) 2460
HapII CCGG 5 cut(s) 222, 411, 576, 804, 2490
HgaI GACGC 1 cut(s) 1060
HhaI GCGC 4 cut(s) 640, 1225, 1985, 2459
Hin6I GCGC 4 cut(s) 638, 1223, 1983, 2457
HinP1I GCGC 4 cut(s) 638, 1223, 1983, 2457
HincII GTYRAC 1 cut(s) 1187
HindII GTYRAC 1 cut(s) 1187
HindIII AAGCTT 1 cut(s) 2648
HinfI GANTC 7 cut(s) 188, 392, 472, 489, 742, 1843, 2524
HpaII CCGG 5 cut(s) 222, 411, 576, 804, 2490
HphI GGTGA 7 cut(s) 73, 108, 281, 498, 1371, 2459, 2496
Hpy166II GTNNAC 9 cut(s) 391, 520, 925, 985, 1180, 1187, 1320, 2666, 2682
Hpy8I GTNNAC 9 cut(s) 391, 520, 925, 985, 1180, 1187, 1320, 2666, 2682
Hpy99I CGWCG 2 cut(s) 9, 1076
HpyAV CCTTC 3 cut(s) 683, 2168, 2597
HpyCH4III ACNGT 9 cut(s) 556, 977, 1184, 2026, 2124, 2224, 2255, 2364, 2670
HpyCH4IV ACGT 3 cut(s) 261, 1487, 2684
HpyF10VI GCNNNNNNNGC 7 cut(s) 10, 305, 637, 842, 948, 1433, 1538
HpyF3I CTNAG 8 cut(s) 321, 720, 967, 1133, 1706, 1725, 2244, 2427
HpySE526I ACGT 3 cut(s) 261, 1487, 2684
HspAI GCGC 4 cut(s) 638, 1223, 1983, 2457
KroI GCCGGC 1 cut(s) 803
KroNI GCCGGC 1 cut(s) 805
Kzo9I GATC 7 cut(s) 113, 1025, 1586, 1637, 2101, 2108, 2194
LmnI GCTCC 7 cut(s) 40, 413, 732, 893, 1071, 1445, 2587
Lsp1109I GCAGC 5 cut(s) 155, 823, 1063, 1261, 1414
LweI GCATC 3 cut(s) 733, 1088, 2404
MaeI CTAG 2 cut(s) 695, 2106
MaeII ACGT 3 cut(s) 261, 1487, 2684
MaeIII GTNAC 7 cut(s) 79, 1524, 2118, 2212, 2255, 2447, 2617
MalI GATC 7 cut(s) 115, 1027, 1588, 1639, 2103, 2110, 2196
MboI GATC 7 cut(s) 113, 1025, 1586, 1637, 2101, 2108, 2194
MfeI CAATTG 1 cut(s) 1019
MflI RGATCY 3 cut(s) 1025, 2101, 2108
MhlI GDGCHC 2 cut(s) 287, 706
MlsI TGGCCA 1 cut(s) 861
MluNI TGGCCA 1 cut(s) 861
MlyI GAGTC 3 cut(s) 197, 386, 2518
MmeI TCCRAC 2 cut(s) 588, 2219
Mox20I TGGCCA 1 cut(s) 861
Mph1103I ATGCAT 1 cut(s) 2295
MroNI GCCGGC 1 cut(s) 803
MroXI GAANNNNTTC 3 cut(s) 2500, 2637, 2641
MscI TGGCCA 1 cut(s) 861
MslI CAYNNNNRTG 4 cut(s) 157, 1313, 1799, 2362
Msp20I TGGCCA 1 cut(s) 861
MspA1I CMGCKG 2 cut(s) 1274, 1522
MspCI CTTAAG 2 cut(s) 2552, 2707
MspI CCGG 5 cut(s) 222, 411, 576, 804, 2490
MspR9I CCNGG 4 cut(s) 218, 1848, 2139, 2491
MunI CAATTG 1 cut(s) 1019
Mva1269I GAATGC 4 cut(s) 1206, 1676, 2405, 2702
MvaI CCWGG 3 cut(s) 218, 1848, 2139
MvnI CGCG 1 cut(s) 1071
MwoI GCNNNNNNNGC 7 cut(s) 10, 305, 637, 842, 948, 1433, 1538
NaeI GCCGGC 1 cut(s) 805
NciI CCSGG 1 cut(s) 2491
NcoI CCATGG 5 cut(s) 89, 728, 768, 1403, 1824
NdeI CATATG 2 cut(s) 1390, 1684
NdeII GATC 7 cut(s) 113, 1025, 1586, 1637, 2101, 2108, 2194
NgoMIV GCCGGC 1 cut(s) 803
NlaIV GGNNCC 8 cut(s) 25, 285, 574, 895, 1621, 1668, 1822, 1954
NmuCI GTSAC 5 cut(s) 79, 1524, 2118, 2255, 2447
NsiI ATGCAT 1 cut(s) 2295
NspI RCATGY 5 cut(s) 656, 1532, 2075, 2297, 2477
PaeI GCATGC 1 cut(s) 656
PaqCI CACCTGC 1 cut(s) 1441
PceI AGGCCT 2 cut(s) 951, 1131
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PctI GAATGC 4 cut(s) 1206, 1676, 2405, 2702
PdiI GCCGGC 1 cut(s) 805
PdmI GAANNNNTTC 3 cut(s) 2500, 2637, 2641
PfeI GAWTC 4 cut(s) 472, 489, 742, 1843
PflFI GACNNNGTC 1 cut(s) 554
PflMI CCANNNNNTGG 1 cut(s) 90
PfoI TCCNGGA 1 cut(s) 2137
PkrI GCNGC 5 cut(s) 145, 838, 1078, 1276, 1429
PleI GAGTC 3 cut(s) 196, 386, 2518
PpsI GAGTC 3 cut(s) 196, 386, 2518
Ppu21I YACGTR 1 cut(s) 1488
PshBI ATTAAT 1 cut(s) 2169
PsiI TTATAA 1 cut(s) 716
Psp6I CCWGG 3 cut(s) 216, 1846, 2137
PspFI CCCAGC 1 cut(s) 1867
PspGI CCWGG 3 cut(s) 216, 1846, 2137
PspN4I GGNNCC 8 cut(s) 25, 285, 574, 895, 1621, 1668, 1822, 1954
PspOMI GGGCCC 1 cut(s) 283
PspPI GGNCC 6 cut(s) 283, 284, 573, 806, 1821, 2535
PstI CTGCAG 2 cut(s) 841, 1279
PstNI CAGNNNCTG 1 cut(s) 2417
PsuI RGATCY 3 cut(s) 1025, 2101, 2108
PsyI GACNNNGTC 1 cut(s) 554
PvuII CAGCTG 2 cut(s) 1274, 1522
RigI GGCCGGCC 1 cut(s) 807
RsaI GTAC 7 cut(s) 739, 1105, 1169, 1767, 1776, 2023, 2028
RsaNI GTAC 7 cut(s) 738, 1104, 1168, 1766, 1775, 2022, 2027
RseI CAYNNNNRTG 4 cut(s) 157, 1313, 1799, 2362
SatI GCNGC 5 cut(s) 144, 837, 1077, 1275, 1428
Sau3AI GATC 7 cut(s) 113, 1025, 1586, 1637, 2101, 2108, 2194
Sau96I GGNCC 6 cut(s) 283, 284, 573, 806, 1821, 2535
ScaI AGTACT 1 cut(s) 1776
SchI GAGTC 3 cut(s) 197, 386, 2518
ScrFI CCNGG 4 cut(s) 218, 1848, 2139, 2491
SduI GDGCHC 2 cut(s) 287, 706
SfaNI GCATC 3 cut(s) 733, 1088, 2404
SfcI CTRYAG 3 cut(s) 837, 1275, 2460
SfiI GGCCNNNNNGGCC 1 cut(s) 948
SinI GGWCC 2 cut(s) 1821, 2535
SmiMI CAYNNNNRTG 4 cut(s) 157, 1313, 1799, 2362
SmlI CTYRAG 4 cut(s) 2310, 2552, 2591, 2707
SmoI CTYRAG 4 cut(s) 2310, 2552, 2591, 2707
SnaBI TACGTA 1 cut(s) 1488
SphI GCATGC 1 cut(s) 656
SseBI AGGCCT 2 cut(s) 951, 1131
SsiI CCGC 3 cut(s) 198, 1069, 2558
SspI AATATT 2 cut(s) 880, 1463
SspMI CTAG 2 cut(s) 695, 2106
StuI AGGCCT 2 cut(s) 951, 1131
StyD4I CCNGG 4 cut(s) 216, 1846, 2137, 2489
StyI CCWWGG 6 cut(s) 89, 728, 768, 815, 1403, 1824
TaaI ACNGT 9 cut(s) 556, 977, 1184, 2026, 2124, 2224, 2255, 2364, 2670
TaiI ACGT 3 cut(s) 264, 1490, 2687
TaqI TCGA 4 cut(s) 186, 1008, 2191, 2631
TatI WGTACW 3 cut(s) 1103, 1167, 1774
TfiI GAWTC 4 cut(s) 472, 489, 742, 1843
TseFI GTSAC 5 cut(s) 79, 1524, 2118, 2255, 2447
TseI GCWGC 5 cut(s) 143, 836, 1076, 1274, 1427
Tsp45I GTSAC 5 cut(s) 79, 1524, 2118, 2255, 2447
TspDTI ATGAA 9 cut(s) 440, 1196, 1472, 1783, 1923, 1931, 2187, 2346, 2403
TspGWI ACGGA 1 cut(s) 22
Tth111I GACNNNGTC 1 cut(s) 554
Van91I CCANNNNNTGG 1 cut(s) 90
Vha464I CTTAAG 2 cut(s) 2552, 2707
VpaK11BI GGWCC 2 cut(s) 1821, 2535
VspI ATTAAT 1 cut(s) 2169
XapI RAATTY 2 cut(s) 1004, 2637
XbaI TCTAGA 1 cut(s) 694
XceI RCATGY 5 cut(s) 656, 1532, 2075, 2297, 2477
XmiI GTMKAC 3 cut(s) 924, 984, 1179
XmnI GAANNNNTTC 3 cut(s) 2500, 2637, 2641
XspI CTAG 2 cut(s) 695, 2106
ZrmI AGTACT 1 cut(s) 1776
Zsp2I ATGCAT 1 cut(s) 2295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.