MD07G1174500.v1.1

Endoplasmic reticulum metallopeptidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011223
Physical Location & Seq
Reverse (-)
25221085 .. 25227098
6014 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1174500.v1.1.491

Sequence Viewer

Length: 2730 bp
ATGCGACGGAGGCCCCAGCAGAGTCCGGCACCCGCCACCGCCAATCCATCACCCACCGGAGCAGAACCAGTGTCCACTTCATGGGTCGGACAGCAGAGGCCTCAGCGGTCACCCTTCGTTTGGCTCACCTTATTCCTGGCCATCGGATACTGTTCTTATGGAGTTTACCAATACCAGTTTGAGAGCTTGCCCGAGCCGCTCACGGCGGACCAGGCCGGAAAGCGCGGCTTCTCTGAGTTTTCAGCTCTCAAACACGTCGAGGCCTTGACCCAATTGGGTCCTCACTCTGTCGGCTCCGATGCTCTCAACCTAGCTCTCCAGTATGTTTTGGCAGAAGCTGAGAAGATCAAGAAAACTGCTCACTGGGAGGTTGTTGTTGAAGTGGACTCGTTCCATGTCGAATCGGGAGCAAATCGAATGGTTGGTGGCCTGTTCAAGGGAAGAACACTTGTATATGCTGATCTAAATCATATTGTTGTAAGAGTTTTGCCAAAGTATGCGCCTGAATCAGTAGATAATGCGATTCTGGTCTCTTCTCACATCGACACCGTCTTCTCAACGGGAGGGGCTGGAGATTGCAGTTCATGTGTAGCCGTCATGTTGGAACTTGCTCGTGGGGTTTCCCAGTGGGCTCATGGATTTAAGCATGCTGTTATATTTTTGTTTAATACTGGGGAGGAGGAGGGTCTAAATGGTGCTCATAGCTTTATAACTCAGCATCCGTGGAGTACAACTATTCGTCTGGCTGTTGATTTGGAGGCTATGGGTATTGGAGGGAAGTCTGGCATATTTCAGGCTGATGCTTGGTCTATTGAGAATTTTGCATCGGTAGCAAAATACCCATCTGGCCAAATTATAGGACAGGATATATTTTCTTCTGGAGCCATAAAATCTGCAACAGATTTCCAAGTATATAAGGAGGTTGCTGGCCTTCCAGGGCTCGACTTCGCTTACACAGATAACACTGCAGTCTACCATACAAAGAATGACAAAATCGAGCTTCATACATTAGGATCTCTTCAGCATCTTGGAGAAAATATGCTTGCTTTTCTACTAAAGATTGCTGCATCCTCTCATCTTCCAAAAGCCAGTGCAGTGGTAGAAGAGAATAAGGGCGAGACTGCTGTCATATATTTTGACATATTGGGGTCATATATGATCGTGTATCGTCAACGCTTTGCAAATATGCTTCATAATTCAGTGATAGCGCAGTCACTTCTGATATGGACTACATCATTGCTTATGGGTGGTTATCCGGCTGCAATTTCATTGGCCTTGTCATGTTTGAGTATCATATTAATGTGGATATTCGCAATAAGTTTCGCTCTTCTTCCTGCCTTCATTATATCACTATTATCTTCTTCACCAGTGCCATATGTTGCAAACCCATGGCTAGTAATTGGGTTATTTGGCGCACCTGCTCTTATTGGGGCATTAAGTGGTCAATATCTGGGTTATCTTGGTCTGCATACATATTTGTCAAAAGCATACGCCAAGAAAAAACAACTCTCAGCTGATATACAAGCTGGTTTGGTTAAATTAGAGGCTGAAAGATGGCTCTACAAATCTGGATCTCTTCAGTGGCTCATTCTCCTCATTTTGGGAACCTATTACAAAATTGGATCAACTTATTTGGCTCTTGCTTGGTTGGTCCCACCAGCATTCGCATATGGCTTTCTTGAAGCGACTCTAACCCCAGCTCGATTCCCCAAGCCACTCAGACTTGCAACCCTGCTGATAGGATTGGCTGTACCAATAGTAATTTCTTCCGGCGGATTTATTCGGCTGGCTGGAATGGTAATTGGGACTGTGGTTCGACTTGACAGGAACCCCGGTGGCACCCCTGAGTGGCTAGCGAATGTTATAGTTGCTGTTTTTGTTGCTGTTGTTGTGTGCCTGACTTTGGTGTATCTCCTTTCATATATTCATCTTTCAGGTGCCAAGAGATCGATTGTCCTTTCAACTTGCATGCTGTTTGGCCTCTCACTTGCTGTTGTATTGTTGGGTATCGTTCCACCATTTACCGCAGACACGTCCAGAGCTGTAAACGTTGTGCATGTTGTAGATACGACAGGAAGCGTTGATGGAAACCAAGATCCTAGATCGTATGTGTCTTTGTTTTCTTTCACTCCTGGAAAGTTGACAGAGGAGGCTGAGCAAATTAATGAAGGTTTCAGATGTGGTAGAGACAAAGTTGTTGATCTTGTAACCTTTTCAGCCAAATACAGTTGTTGGACTTATGATGACGGTGACAGTGGGTGGGGCAAATCAGATATACCCACAATGAACGTTGGCAGCGATACTCATGGTAATGAAAGAACAACACGAGTACTACTTGATACAAAAGGTTCAACACGCTGGACGCTTGCTATCAACGCTGATGACATAGAAGATTTCACATTCAAAGGAAGCTCGGAGGAATTGGCTTCTTTGGGTGACATGAGTAGCGTAGATGGATGGCACATTATCCAGTATTCCGGAGGACAAAATGCACCAACAAGGTTCGATCTAGCACTCTTCTGGTCACAAAACTCTACCCGGCTGGCTCACAATGTTGAAGGGAAGAGAGACGAGCACGCTCCCCTTCTAAAGTTAAGAACTGATGTGGACATAGTGACTCCTAAAGTCGAAAGAGTTCTTTCGAAGCTTCCTCCTTGGTGTTCGCTGTTTGGCAAGTCGACGTCCCCCCACACCCTTGCATTTTTGAGCAATCTTCCTGTTAATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M28

Protein Analysis

910

Amino Acids

98.78

Weight (kDa)

6.38

Isoelectric Point (pI)

34.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M28 PF04389 163 - 350 1.8e-44 Peptidase family M28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011223)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G20660 AT5G20660 AT5G20660 AT5G20660
fragaria_vesca FvH4_7g19460 FvH4_7g19460 FvH4_7g19460 FvH4_7g19460
malus_domestica MD07G1174500.v1.1
prunus_persica Prupe.2G213300_v2.0.a1 Prupe.2G213300_v2.0.a1 Prupe.2G213300_v2.0.a1
pyrus_communis pycom07g16870
rosa_chinensis RchiOBHm_Chr1g0363331
rosa_laevigata RLG00000027573
rosa_roxburghii Rroxscaffold_4G00293270
rosa_rugosa Rorug01G0306400
rosa_samantha Rh1AG315200 Rh1CG294900
rosa_wichuraiana Rw1G027930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 710
AarI CACCTGC 1 cut(s) 1426
AatII GACGTC 1 cut(s) 2684
Acc36I ACCTGC 1 cut(s) 1426
AccB1I GGYRCC 3 cut(s) 28, 1838, 1937
AccB7I CCANNNNNTGG 1 cut(s) 81
AccBSI CCGCTC 1 cut(s) 199
AccI GTMKAC 2 cut(s) 972, 2678
AccII CGCG 1 cut(s) 225
AccIII TCCGGA 1 cut(s) 2477
AciI CCGC 8 cut(s) 33, 39, 106, 197, 206, 225, 1773, 2025
AclI AACGTT 2 cut(s) 2049, 2289
AclWI GGATC 4 cut(s) 1021, 1579, 1630, 2090
AcoI YGGCCR 2 cut(s) 138, 847
AcsI RAATTY 1 cut(s) 817
AcuI CTGAAG 2 cut(s) 1004, 1562
AcyI GRCGYC 1 cut(s) 2681
AfaI GTAC 3 cut(s) 730, 1752, 2331
AfiI CCNNNNNNNGG 6 cut(s) 81, 120, 436, 1600, 1848, 1903
AflIII ACRYGT 2 cut(s) 253, 2031
AgsI TTSAA 7 cut(s) 380, 436, 1682, 1962, 2352, 2404, 2558
AjiI CACGTC 2 cut(s) 256, 2034
AjnI CCWGG 4 cut(s) 135, 210, 934, 2131
AjuI GAANNNNNNNTTGG 2 cut(s) 1747, 1779
Alw21I GWGCWC 2 cut(s) 700, 2577
Alw26I GTCTC 4 cut(s) 535, 1112, 2181, 2562
AlwI GGATC 4 cut(s) 1021, 1579, 1630, 2090
AlwNI CAGNNNCTG 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 191
Aor13HI TCCGGA 1 cut(s) 2477
ApeKI GCWGC 3 cut(s) 1064, 1259, 2295
ApoI RAATTY 1 cut(s) 817
AseI ATTAAT 2 cut(s) 1298, 2163
Asp700I GAANNNNTTC 1 cut(s) 2634
AspLEI GCGC 4 cut(s) 225, 502, 1210, 1415
AspS9I GGNCC 4 cut(s) 12, 208, 278, 1651
AsuC2I CCSGG 2 cut(s) 1833, 2539
AsuHPI GGTGA 6 cut(s) 42, 102, 118, 1356, 2261, 2447
AsuII TTCGAA 1 cut(s) 2642
AsuNHI GCTAGC 1 cut(s) 1852
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 3 cut(s) 208, 278, 1651
BalI TGGCCA 2 cut(s) 140, 849
BanI GGYRCC 3 cut(s) 28, 1838, 1937
BanII GRGCYC 2 cut(s) 634, 942
BauI CACGAG 2 cut(s) 612, 2325
BbsI GAAGAC 1 cut(s) 544
Bbv12I GWGCWC 2 cut(s) 700, 2577
BbvCI CCTCAGC 1 cut(s) 102
BbvI GCAGC 3 cut(s) 1051, 1246, 2307
BccI CCATC 7 cut(s) 55, 149, 850, 1548, 2078, 2447, 2451
BceAI ACGGC 2 cut(s) 219, 578
BciT130I CCWGG 4 cut(s) 137, 212, 936, 2133
BciVI GTATCC 1 cut(s) 140
BcnI CCSGG 2 cut(s) 1833, 2539
BcoDI GTCTC 4 cut(s) 535, 1112, 2181, 2562
BfaI CTAG 5 cut(s) 311, 1394, 1853, 2100, 2510
BfmI CTRYAG 1 cut(s) 966
BfuAI ACCTGC 1 cut(s) 1426
BfuI GTATCC 1 cut(s) 140
BisI GCNGC 5 cut(s) 197, 226, 1065, 1260, 2296
BlpI GCTNAGC 1 cut(s) 2154
BlsI GCNGC 5 cut(s) 198, 227, 1066, 1261, 2297
BmcAI AGTACT 1 cut(s) 2331
Bme1390I CCNGG 6 cut(s) 137, 212, 936, 1833, 2133, 2539
Bme18I GGWCC 3 cut(s) 208, 278, 1651
BmeT110I CYCGRG 1 cut(s) 191
BmgBI CACGTC 2 cut(s) 256, 2034
BmgT120I GGNCC 4 cut(s) 12, 208, 278, 1651
BmrFI CCNGG 6 cut(s) 137, 212, 936, 1833, 2133, 2539
BmrI ACTGGG 3 cut(s) 373, 619, 681
BmsI GCATC 6 cut(s) 289, 727, 790, 833, 1033, 1076
BmtI GCTAGC 1 cut(s) 1856
BmuI ACTGGG 3 cut(s) 373, 619, 681
BoxI GACNNNNGTC 1 cut(s) 1124
BpiI GAAGAC 1 cut(s) 544
BpmI CTGGAG 3 cut(s) 302, 591, 900
Bpu10I CCTNAGC 1 cut(s) 102
Bpu1102I GCTNAGC 1 cut(s) 2154
Bpu14I TTCGAA 1 cut(s) 2642
BpuMI CCSGG 2 cut(s) 1833, 2539
Bsa29I ATCGAT 1 cut(s) 1949
BsaBI GATNNNNATC 1 cut(s) 465
BsaHI GRCGYC 1 cut(s) 2681
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 5 cut(s) 722, 935, 1388, 1831, 2654
BsaWI WCCGGW 2 cut(s) 56, 2477
Bsc4I CCNNNNNNNGG 6 cut(s) 81, 120, 436, 1600, 1848, 1903
Bse1I ACTGG 9 cut(s) 68, 175, 319, 368, 625, 676, 1089, 1367, 2470
Bse3DI GCAATG 1 cut(s) 1235
Bse8I GATNNNNATC 1 cut(s) 465
BseAI TCCGGA 1 cut(s) 2477
BseBI CCWGG 4 cut(s) 137, 212, 936, 2133
BseCI ATCGAT 1 cut(s) 1949
BseDI CCNNGG 5 cut(s) 722, 935, 1388, 1831, 2654
BseGI GGATG 3 cut(s) 718, 1067, 2462
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 6 cut(s) 81, 120, 436, 1600, 1848, 1903
BseMI GCAATG 1 cut(s) 1235
BseMII CTCAG 8 cut(s) 116, 225, 330, 728, 1524, 1732, 1836, 2145
BseNI ACTGG 9 cut(s) 68, 175, 319, 368, 625, 676, 1089, 1367, 2470
BseRI GAGGAG 4 cut(s) 692, 695, 1583, 2162
BseXI GCAGC 3 cut(s) 1051, 1246, 2307
BseYI CCCAGC 2 cut(s) 15, 1696
BsgI GTGCAG 1 cut(s) 1113
Bsh1236I CGCG 1 cut(s) 225
BshNI GGYRCC 3 cut(s) 28, 1838, 1937
BshVI ATCGAT 1 cut(s) 1949
BsiHKAI GWGCWC 2 cut(s) 700, 2577
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 8 cut(s) 26, 57, 216, 1256, 1770, 1833, 2478, 2539
BslFI GGGAC 3 cut(s) 1637, 1819, 2668
BslI CCNNNNNNNGG 6 cut(s) 81, 120, 436, 1600, 1848, 1903
BsmAI GTCTC 4 cut(s) 535, 1112, 2181, 2562
BsmBI CGTCTC 1 cut(s) 2562
BsmFI GGGAC 3 cut(s) 1637, 1819, 2668
BsmI GAATGC 1 cut(s) 1661
Bso31I GGTCTC 1 cut(s) 535
BsoBI CYCGRG 1 cut(s) 191
Bsp119I TTCGAA 1 cut(s) 2642
Bsp1286I GDGCHC 4 cut(s) 634, 700, 942, 2577
Bsp13I TCCGGA 1 cut(s) 2477
Bsp1720I GCTNAGC 1 cut(s) 2154
Bsp19I CCATGG 1 cut(s) 1388
BspACI CCGC 8 cut(s) 33, 39, 106, 197, 206, 225, 1773, 2025
BspCNI CTCAG 8 cut(s) 115, 226, 331, 727, 1523, 1731, 1837, 2146
BspDI ATCGAT 1 cut(s) 1949
BspEI TCCGGA 1 cut(s) 2477
BspFNI CGCG 1 cut(s) 225
BspMAI CTGCAG 1 cut(s) 970
BspMI ACCTGC 1 cut(s) 1426
BspOI GCTAGC 1 cut(s) 1856
BspPI GGATC 4 cut(s) 1021, 1579, 1630, 2090
BspQI GCTCTTC 1 cut(s) 1332
BspT104I TTCGAA 1 cut(s) 2642
BspT107I GGYRCC 3 cut(s) 28, 1838, 1937
BspTNI GGTCTC 1 cut(s) 535
BsrBI CCGCTC 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 1235
BsrI ACTGG 9 cut(s) 68, 175, 319, 368, 625, 676, 1089, 1367, 2470
BssECI CCNNGG 5 cut(s) 722, 935, 1388, 1831, 2654
BssNI GRCGYC 1 cut(s) 2681
BssSI CACGAG 2 cut(s) 612, 2325
BssT1I CCWWGG 2 cut(s) 1388, 2654
Bst2BI CACGAG 2 cut(s) 612, 2325
Bst2UI CCWGG 4 cut(s) 137, 212, 936, 2133
Bst4CI ACNGT 6 cut(s) 152, 550, 1810, 2228, 2249, 2255
Bst6I CTCTTC 7 cut(s) 538, 1023, 1098, 1332, 1581, 2522, 2558
BstACI GRCGYC 1 cut(s) 2681
BstBI TTCGAA 1 cut(s) 2642
BstDEI CTNAG 8 cut(s) 102, 234, 339, 714, 1510, 1718, 1845, 2154
BstDSI CCRYGG 2 cut(s) 722, 1388
BstEII GGTNACC 1 cut(s) 108
BstENI CCTNNNNNAGG 1 cut(s) 434
BstF5I GGATG 3 cut(s) 718, 1067, 2462
BstFNI CGCG 1 cut(s) 225
BstHHI GCGC 4 cut(s) 225, 502, 1210, 1415
BstMAI GTCTC 4 cut(s) 535, 1112, 2181, 2562
BstMWI GCNNNNNNNGC 5 cut(s) 10, 196, 212, 830, 2375
BstNI CCWGG 4 cut(s) 137, 212, 936, 2133
BstNSI RCATGY 3 cut(s) 650, 1972, 2060
BstPAI GACNNNNGTC 1 cut(s) 1124
BstPI GGTNACC 1 cut(s) 108
BstSCI CCNGG 6 cut(s) 135, 210, 934, 1831, 2131, 2537
BstSFI CTRYAG 1 cut(s) 966
BstUI CGCG 1 cut(s) 225
BstV1I GCAGC 3 cut(s) 1051, 1246, 2307
BstV2I GAAGAC 1 cut(s) 544
BstX2I RGATCY 3 cut(s) 1013, 1571, 2095
BstXI CCANNNNNNTGG 1 cut(s) 1096
BstYI RGATCY 3 cut(s) 1013, 1571, 2095
Bsu15I ATCGAT 1 cut(s) 1949
BsuI GTATCC 1 cut(s) 140
BsuTUI ATCGAT 1 cut(s) 1949
BtgI CCRYGG 2 cut(s) 722, 1388
BtrI CACGTC 2 cut(s) 256, 2034
BtsCI GGATG 3 cut(s) 718, 1067, 2462
BtsI GCAGTG 2 cut(s) 963, 1101
BveI ACCTGC 1 cut(s) 1426
CaiI CAGNNNCTG 1 cut(s) 338
CfoI GCGC 4 cut(s) 225, 502, 1210, 1415
Cfr13I GGNCC 4 cut(s) 12, 208, 278, 1651
ClaI ATCGAT 1 cut(s) 1949
CseI GACGC 1 cut(s) 2371
Csp6I GTAC 3 cut(s) 729, 1751, 2330
CviQI GTAC 3 cut(s) 729, 1751, 2330
DdeI CTNAG 8 cut(s) 102, 234, 339, 714, 1510, 1718, 1845, 2154
EaeI YGGCCR 2 cut(s) 138, 847
Eam1104I CTCTTC 7 cut(s) 538, 1023, 1098, 1332, 1581, 2522, 2558
EarI CTCTTC 7 cut(s) 538, 1023, 1098, 1332, 1581, 2522, 2558
EciI GGCGGA 2 cut(s) 221, 1788
Eco130I CCWWGG 2 cut(s) 1388, 2654
Eco147I AGGCCT 2 cut(s) 100, 263
Eco24I GRGCYC 2 cut(s) 634, 942
Eco31I GGTCTC 1 cut(s) 535
Eco47I GGWCC 3 cut(s) 208, 278, 1651
Eco57I CTGAAG 2 cut(s) 1004, 1562
Eco88I CYCGRG 1 cut(s) 191
Eco91I GGTNACC 1 cut(s) 108
EcoNI CCTNNNNNAGG 1 cut(s) 434
EcoO109I RGGNCCY 2 cut(s) 12, 278
EcoO65I GGTNACC 1 cut(s) 108
EcoRII CCWGG 4 cut(s) 135, 210, 934, 2131
EcoT14I CCWWGG 2 cut(s) 1388, 2654
EcoT38I GRGCYC 2 cut(s) 634, 942
ErhI CCWWGG 2 cut(s) 1388, 2654
Esp3I CGTCTC 1 cut(s) 2562
FalI AAGNNNNNCTT 2 cut(s) 212, 244
FaqI GGGAC 3 cut(s) 1637, 1819, 2668
FauI CCCGC 1 cut(s) 40
FauNDI CATATG 2 cut(s) 1375, 1669
FblI GTMKAC 2 cut(s) 972, 2678
Fnu4HI GCNGC 5 cut(s) 197, 226, 1065, 1260, 2296
FokI GGATG 3 cut(s) 705, 1054, 2469
FriOI GRGCYC 2 cut(s) 634, 942
Fsp4HI GCNGC 5 cut(s) 197, 226, 1065, 1260, 2296
FspBI CTAG 5 cut(s) 311, 1394, 1853, 2100, 2510
GlaI GCGC 4 cut(s) 224, 501, 1209, 1414
GluI GCNGC 5 cut(s) 197, 226, 1065, 1260, 2296
GsaI CCCAGC 2 cut(s) 19, 1700
GsuI CTGGAG 3 cut(s) 302, 591, 900
HapII CCGG 8 cut(s) 26, 57, 216, 1256, 1770, 1833, 2478, 2539
HgaI GACGC 1 cut(s) 2371
HhaI GCGC 4 cut(s) 225, 502, 1210, 1415
Hin1I GRCGYC 1 cut(s) 2681
Hin6I GCGC 4 cut(s) 223, 500, 1208, 1413
HinP1I GCGC 4 cut(s) 223, 500, 1208, 1413
HincII GTYRAC 3 cut(s) 1172, 2142, 2679
HindII GTYRAC 3 cut(s) 1172, 2142, 2679
HindIII AAGCTT 1 cut(s) 2645
HinfI GANTC 8 cut(s) 22, 386, 401, 506, 523, 1687, 1704, 2617
HpaII CCGG 8 cut(s) 26, 57, 216, 1256, 1770, 1833, 2478, 2539
HphI GGTGA 6 cut(s) 42, 102, 118, 1356, 2261, 2447
Hpy166II GTNNAC 9 cut(s) 75, 166, 385, 973, 1172, 2047, 2142, 2608, 2679
Hpy188I TCNGA 9 cut(s) 89, 146, 235, 298, 1221, 1721, 2177, 2272, 2416
Hpy188III TCNNGA 7 cut(s) 349, 405, 879, 1569, 1679, 2037, 2478
Hpy8I GTNNAC 9 cut(s) 75, 166, 385, 973, 1172, 2047, 2142, 2608, 2679
Hpy99I CGWCG 3 cut(s) 9, 260, 2683
HpyAV CCTTC 6 cut(s) 124, 941, 1348, 2162, 2552, 2594
HpyCH4III ACNGT 6 cut(s) 152, 550, 1810, 2228, 2249, 2255
HpyCH4IV ACGT 5 cut(s) 255, 2033, 2049, 2289, 2681
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 196, 212, 830, 2375
HpyF3I CTNAG 8 cut(s) 102, 234, 339, 714, 1510, 1718, 1845, 2154
HpySE526I ACGT 5 cut(s) 255, 2033, 2049, 2289, 2681
Hsp92I GRCGYC 1 cut(s) 2681
HspAI GCGC 4 cut(s) 223, 500, 1208, 1413
Kpn2I TCCGGA 1 cut(s) 2477
LguI GCTCTTC 1 cut(s) 1332
LmnI GCTCC 5 cut(s) 59, 299, 407, 881, 2584
Lsp1109I GCAGC 3 cut(s) 1051, 1246, 2307
LweI GCATC 6 cut(s) 289, 727, 790, 833, 1033, 1076
MaeI CTAG 5 cut(s) 311, 1394, 1853, 2100, 2510
MaeII ACGT 5 cut(s) 255, 2033, 2049, 2289, 2681
MaeIII GTNAC 7 cut(s) 108, 1212, 2206, 2249, 2435, 2523, 2614
MbiI CCGCTC 1 cut(s) 199
MfeI CAATTG 1 cut(s) 272
MflI RGATCY 3 cut(s) 1013, 1571, 2095
MhlI GDGCHC 4 cut(s) 634, 700, 942, 2577
MlsI TGGCCA 2 cut(s) 140, 849
MluNI TGGCCA 2 cut(s) 140, 849
MlyI GAGTC 4 cut(s) 31, 380, 1681, 2611
MmeI TCCRAC 3 cut(s) 67, 582, 2213
Mox20I TGGCCA 2 cut(s) 140, 849
MroI TCCGGA 1 cut(s) 2477
MroXI GAANNNNTTC 1 cut(s) 2634
MscI TGGCCA 2 cut(s) 140, 849
MseI TTAA 8 cut(s) 642, 666, 1298, 1436, 1536, 2163, 2594, 2721
MslI CAYNNNNRTG 2 cut(s) 1298, 2310
Msp20I TGGCCA 2 cut(s) 140, 849
MspA1I CMGCKG 2 cut(s) 106, 1514
MspI CCGG 8 cut(s) 26, 57, 216, 1256, 1770, 1833, 2478, 2539
MspR9I CCNGG 6 cut(s) 137, 212, 936, 1833, 2133, 2539
MunI CAATTG 1 cut(s) 272
Mva1269I GAATGC 1 cut(s) 1661
MvaI CCWGG 4 cut(s) 137, 212, 936, 2133
MvnI CGCG 1 cut(s) 225
MwoI GCNNNNNNNGC 5 cut(s) 10, 196, 212, 830, 2375
NciI CCSGG 2 cut(s) 1833, 2539
NcoI CCATGG 1 cut(s) 1388
NdeI CATATG 2 cut(s) 1375, 1669
NheI GCTAGC 1 cut(s) 1852
NmuCI GTSAC 6 cut(s) 108, 1212, 2249, 2435, 2523, 2614
NspI RCATGY 3 cut(s) 650, 1972, 2060
NspV TTCGAA 1 cut(s) 2642
PaeI GCATGC 2 cut(s) 650, 1972
PaqCI CACCTGC 1 cut(s) 1426
PceI AGGCCT 2 cut(s) 100, 263
PciSI GCTCTTC 1 cut(s) 1332
PcsI WCGNNNNNNNCGW 1 cut(s) 2295
PctI GAATGC 1 cut(s) 1661
PdmI GAANNNNTTC 1 cut(s) 2634
PfeI GAWTC 4 cut(s) 401, 506, 523, 1704
PflFI GACNNNGTC 1 cut(s) 548
PflMI CCANNNNNTGG 1 cut(s) 81
PfoI TCCNGGA 1 cut(s) 2131
PkrI GCNGC 5 cut(s) 198, 227, 1066, 1261, 2297
PleI GAGTC 4 cut(s) 30, 380, 1681, 2611
PpsI GAGTC 4 cut(s) 30, 380, 1681, 2611
PpuMI RGGWCCY 1 cut(s) 278
PshAI GACNNNNGTC 1 cut(s) 1124
PshBI ATTAAT 2 cut(s) 1298, 2163
PsiI TTATAA 1 cut(s) 710
Psp1406I AACGTT 2 cut(s) 2049, 2289
Psp5II RGGWCCY 1 cut(s) 278
Psp6I CCWGG 4 cut(s) 135, 210, 934, 2131
PspEI GGTNACC 1 cut(s) 108
PspFI CCCAGC 2 cut(s) 15, 1696
PspGI CCWGG 4 cut(s) 135, 210, 934, 2131
PspPI GGNCC 4 cut(s) 12, 208, 278, 1651
PspPPI RGGWCCY 1 cut(s) 278
PstI CTGCAG 1 cut(s) 970
PstNI CAGNNNCTG 1 cut(s) 338
PsuI RGATCY 3 cut(s) 1013, 1571, 2095
PsyI GACNNNGTC 1 cut(s) 548
PvuII CAGCTG 1 cut(s) 1514
RsaI GTAC 3 cut(s) 730, 1752, 2331
RsaNI GTAC 3 cut(s) 729, 1751, 2330
RseI CAYNNNNRTG 2 cut(s) 1298, 2310
SalI GTCGAC 1 cut(s) 2677
SapI GCTCTTC 1 cut(s) 1332
SaqAI TTAA 8 cut(s) 642, 666, 1298, 1436, 1536, 2163, 2594, 2721
SatI GCNGC 5 cut(s) 197, 226, 1065, 1260, 2296
Sau96I GGNCC 4 cut(s) 12, 208, 278, 1651
ScaI AGTACT 1 cut(s) 2331
SchI GAGTC 4 cut(s) 31, 380, 1681, 2611
ScrFI CCNGG 6 cut(s) 137, 212, 936, 1833, 2133, 2539
SduI GDGCHC 4 cut(s) 634, 700, 942, 2577
SfaNI GCATC 6 cut(s) 289, 727, 790, 833, 1033, 1076
SfcI CTRYAG 1 cut(s) 966
SfuI TTCGAA 1 cut(s) 2642
SinI GGWCC 3 cut(s) 208, 278, 1651
SmiMI CAYNNNNRTG 2 cut(s) 1298, 2310
SphI GCATGC 2 cut(s) 650, 1972
SseBI AGGCCT 2 cut(s) 100, 263
SsiI CCGC 8 cut(s) 33, 39, 106, 197, 206, 225, 1773, 2025
SspMI CTAG 5 cut(s) 311, 1394, 1853, 2100, 2510
StuI AGGCCT 2 cut(s) 100, 263
StyD4I CCNGG 6 cut(s) 135, 210, 934, 1831, 2131, 2537
StyI CCWWGG 2 cut(s) 1388, 2654
TaaI ACNGT 6 cut(s) 152, 550, 1810, 2228, 2249, 2255
TaiI ACGT 5 cut(s) 258, 2036, 2052, 2292, 2684
TatI WGTACW 2 cut(s) 728, 2329
TauI GCSGC 2 cut(s) 199, 228
TfiI GAWTC 4 cut(s) 401, 506, 523, 1704
Tru1I TTAA 8 cut(s) 642, 666, 1298, 1436, 1536, 2163, 2594, 2721
Tru9I TTAA 8 cut(s) 642, 666, 1298, 1436, 1536, 2163, 2594, 2721
TseFI GTSAC 6 cut(s) 108, 1212, 2249, 2435, 2523, 2614
TseI GCWGC 3 cut(s) 1064, 1259, 2295
Tsp45I GTSAC 6 cut(s) 108, 1212, 2249, 2435, 2523, 2614
TspGWI ACGGA 2 cut(s) 22, 711
Tth111I GACNNNGTC 1 cut(s) 548
Van91I CCANNNNNTGG 1 cut(s) 81
VpaK11BI GGWCC 3 cut(s) 208, 278, 1651
VspI ATTAAT 2 cut(s) 1298, 2163
XagI CCTNNNNNAGG 1 cut(s) 434
XapI RAATTY 1 cut(s) 817
XceI RCATGY 3 cut(s) 650, 1972, 2060
XcmI CCANNNNNNNNNTGG 1 cut(s) 632
XmiI GTMKAC 2 cut(s) 972, 2678
XmnI GAANNNNTTC 1 cut(s) 2634
XspI CTAG 5 cut(s) 311, 1394, 1853, 2100, 2510
ZraI GACGTC 1 cut(s) 2682
ZrmI AGTACT 1 cut(s) 2331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.